DRAMP_ID	Sequence	Hiden_Sequence	Original_Sequence	Sequence_Length	Name	Uniprot_Entry	Family	Source	Activity	Protein_Existence	Secondary_Structure	Structure_Description	PDB_ID	Comments	Target_Organism	Hemolytic_Activity	Linear/Cyclic	N_terminal_Modification	C_terminal_Modification	Special_Amino_Acid_and_Stapling_Position	Stereochemistry	Cytotoxicity	Pubmed_ID	Reference	Author	Title	Specific_Type	Nucleotide_Sequence	Full_Sequence	lfcMLE	padj	Workflow
DRAMP21468	KFFⓀKLKⓀAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	20	peptide 2 (derived from OH-CM6)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Random coils in PBS.	①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 8 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 2 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 16 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 16 μg/mL)	[Ref.32216308] It has 1.9% hemolysis against human red blood cells at peptide concentration of 320 μg/mL	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 4 and 8) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (4) and Ⓚ (8) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	No cytotoxicity information found in the reference	32216308	J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.	Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang	Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides	Stapled AMP					
DRAMP21469	KFFⓀKLKKAVⓀKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	20	peptide 3 (derived from OH-CM6)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Random coils in PBS.	①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 8 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 8 μg/mL), Listeria monocytogenes (MIC99.9= 8 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 16 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL)	[Ref.32216308] It has 1.1% hemolysis against red blood cells at peptide concentration of 320 μg/mL	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 4 and 11) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (4) and Ⓚ (11) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	No cytotoxicity information found in the reference	32216308	J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.	Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang	Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides	Stapled AMP					
DRAMP21470	KFFKⓀLKKAVKⓀGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	20	peptide 4 (derived from OH-CM6)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Random coils in PBS.	①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 8 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 8 μg/mL), Listeria monocytogenes (MIC99.9= 4 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL)	[Ref.32216308] It has 1.3% hemolysis against red blood cells at peptide concentration of 320 μg/mL	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 5 and 12) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (5) and Ⓚ (12) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	No cytotoxicity information found in the reference	32216308	J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.	Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang	Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides	Stapled AMP					
DRAMP21471	KFFKKLⓀKAVⓀKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	20	peptide 5 (derived from OH-CM6)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Random coils in PBS.	①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 8 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 8 μg/mL), Listeria monocytogenes (MIC99.9= 2 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 4 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 16 μg/mL)	[Ref.32216308] It has 5.4% hemolysis against red blood cells at peptide concentration of 320 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 7 and 11) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (7) and Ⓚ (11) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	No cytotoxicity information found in the reference	32216308	J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.	Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang	Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides	Stapled AMP					
DRAMP21472	KFFKKLKⓀAVKⓀGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	20	peptide 6 (derived from OH-CM6)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Random coils in PBS.	①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 2 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL)	[Ref.32216308] It has 2.9% hemolysis against red blood cells at peptide concentration of 320 μg/mL	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 8 and 12) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (8) and Ⓚ (12) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	No cytotoxicity information found in the reference	32216308	J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.	Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang	Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides	Stapled AMP					
DRAMP21473	KFFKKLKⓀAVKKGFⓀKFAKV	KFFKKLKKAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	20	peptide 7 (derived from OH-CM6)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Random coils in PBS.	①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 16 μg/mL), Listeria monocytogenes (MIC99.9= 8 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 16 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL)	[Ref.32216308] It has 2.6% hemolysis against red blood cells at peptide concentration of 320 μg/mL	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 8 and 15) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (8) and Ⓚ (15) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	No cytotoxicity information found in the reference	32216308	J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.	Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang	Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides	Stapled AMP					
DRAMP21474	KFFKKLKKAVⓀKGFⓀKFAKV	KFFKKLKKAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	20	peptide 8 (derived from OH-CM6)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Random coils in PBS.	①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 2 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL)	[Ref.32216308] It has 2.7% hemolysis against red blood cells at peptide concentration of 320 μg/mL	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 11 and 15) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (11) and Ⓚ (15) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	No cytotoxicity information found in the reference	32216308	J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.	Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang	Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides	Stapled AMP					
DRAMP21475	KFFKKLKKAVKⓀGFKKFAⓀV	KFFKKLKKAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	20	peptide 9 (derived from OH-CM6)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Random coils in PBS.	①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 8 μg/mL), Listeria monocytogenes (MIC99.9= 4 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 16 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 16 μg/mL)	[Ref.32216308] It has 5.4% hemolysis against red blood cells at peptide concentration of 320 μg/mL	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 12 and 19) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (12) and Ⓚ (19) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	No cytotoxicity information found in the reference	32216308	J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.	Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang	Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides	Stapled AMP					
DRAMP21476	KFFKKLKKAVKⓀGFKⓀFAKV	KFFKKLKKAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	20	peptide 10 (derived from OH-CM6)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①41% α-helical content in 30 mM SDS. ②Random coils in PBS	①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS.  ②Stapled peptides 10 and 12 had a high content of α-helix of about 41 and 45%, respectively, respectively, which could partly explain their strong antibacterial activity and proteolytic resistance.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 2 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL)	[Ref.32216308] It has 2.8% hemolysis against red blood cells at peptide concentration of 320 μg/mL	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 12 and 16) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (12) and Ⓚ (16) are cross-linked by a (E)-but-2-enyl space employing the N-alkylation reactionr.	L	[Ref.32216308] The cell survial of HEK 293T cell line induced by peptide 10 is 101.8%, 100.7%, 98.9%, 89.1% and 83.9%.	32216308	J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.	Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang	Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides	Stapled AMP					
DRAMP21477	KFFKKLKKAVKKGFⓀKFAⓀV	KFFKKLKKAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	20	peptide 11 (derived from OH-CM6)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Random coils in PBS.	①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 8 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 2 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 4 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 16 μg/mL)	[Ref.32216308] It has 0.5% hemolysis against red blood cells at peptide concentration of 320 μg/mL	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 15 and 19) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (15) and Ⓚ (19) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	No cytotoxicity information found in the reference	32216308	J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.	Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang	Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides	Stapled AMP					
DRAMP21478	KFFKKLKKAVKⓀGFKⓀFAKV	KFFKKLKKAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	20	peptide 12 (derived from OH-CM6)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①45% α-helical content in 30 mM SDS. ②Random coils in PBS.	①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS.  ②Stapled peptides 10 and 12 had a high content of α-helix of about 41 and 45%, respectively, respectively, which could partly explain their strong antibacterial activity and proteolytic resistance.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 2 μg/mL), Listeria monocytogenes (MIC99.9= 4 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 4 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 8 μg/mL)	[Ref.32216308] It has 8.7% hemolysis against red blood cells at peptide concentration of 320 μg/mL	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 12 and 16) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (12) and Ⓚ (16) are cross-linked by a 1,2-bismethylenebenzene spacer employing the N-alkylation.	L	[Ref.32216308] The cell survial of HEK 293T cell line induced by peptide 12 is 102.9%, 101.8%, 101.5%, 66.4% and 62.8%.	32216308	J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.	Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang	Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides	Stapled AMP					
DRAMP21479	KFFKKLKKAVKⓀGFKⓀFAKV	KFFKKLKKAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	20	peptide 13 (derived from OH-CM6)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Random coils in PBS.	①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 4 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL)	[Ref.32216308] It has 6.1% hemolysis against red blood cells at peptide concentration of 320 μg/mL	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 12 and 16) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (12) and Ⓚ (16) are cross-linked by a 1,3-bismethylenebenzene spacer employing the N-alkylation.	L	No cytotoxicity information found in the reference	32216308	J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.	Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang	Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides	Stapled AMP					
DRAMP21480	KFFKKLKKAVKⓀGFKⓀFAKV	KFFKKLKKAVKKGFKKFAKV	KFFKKLKKAVKKGFKKFAKV	20	peptide 14 (derived from OH-CM6)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Random coils in PBS.	①All the peptides were random coils in PBS but displayed varied levels of α-helicity in the presence of 30 mM SDS. ②Other stapled peptides had an α-helix content ranging from 16 to 38%, but their antibacterial activity and proteolytic stability were quite similar.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.32216308] Gram-positive bacteria: Staphylococcus aureus (MIC99.9= 4 μg/mL), methicillin-resistant Staphylococcus aureus (MIC99.9= 4 μg/mL), Listeria monocytogenes (MIC99.9= 4 μg/mL);##Gram-negative bacteria: E.coli (MIC99.9= 8 μg/mL), Pseudomonas aeruginosa (MIC99.9= 8 μg/mL), clinically isolated drug-resistant E.coli (MIC99.9= 32 μg/mL)	[Ref.32216308] It has 6.8% hemolysis against red blood cells at peptide concentration of 320 μg/mL	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 12 and 16) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (12) and Ⓚ (16) are cross-linked by a 1,4-bismethylenebenzene spacer employing the N-alkylation.	L	No cytotoxicity information found in the reference	32216308	J Med Chem. 2020 Apr 23;63(8):4081-4089. doi: 10.1021/acs.jmedchem.9b02025. Epub 2020 Apr 8.	Hong Li, Yuchen Hu, Qi Pu, Tong He, Qianyu Zhang, Wen Wu, Xuefeng Xia and Jinqiang Zhang	Novel Stapling by Lysine Tethering Provides Stable and Low Hemolytic Cationic Antimicrobial Peptides	Stapled AMP					
DRAMP21482	KKKKKKAAFⓍAWAⓍFAA	KKKKKKAAFXAWAXFAA	KKKKKKAAFAAWAAFAA	17	S-6K-F17	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		①Random coils with only a small amount of helical structure in aqueous buffer. ②α-helix in SDS detergent micelles.	①Similarly, the stapled peptide, S-6K-F17 is predominantly random coil in aqueous buffer with only a small amount of helical structure despite the presence of the staple - a feature likely due to the large stretch of non-helical Lys residues that flank the stapled portion of the sequence. ②As expected, in detergent micelles S-6K-F17 adopts a helical structure, paralleling the unstapled peptide.	Not found	Function: Antibacterial activity against Gram-negative bacteria. No experiments about antibacterial activity against Gram-positive bacteria are recorded.	[Ref.29275987] Gram-negative bacteria: E. coli (MIC= 1.0 μM)	[Ref.29275987] MHC = 3.8 μM against human red blood cells. Note: Minimum hemolytic concentration (MHC) is the minimum peptide concentration at which red blood cells undergo > 2% hemolysis.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 10 and 14) in sequence indicates 2-(4'-pentenyl) alanine. ②Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	29275987	Bioorg Med Chem. 2018 Mar 15;26(6):1189-1196. doi: 10.1016/j.bmc.2017.10.020. Epub 2017 Oct 21.	Tracy A Stone, Gregory B Cole, Huong Q Nguyen, Simon Sharpe, Charles M Deber	Influence of hydrocarbon-stapling on membrane interactions of synthetic antimicrobial peptides	Stapled AMP					
DRAMP21483	KKKKKKAGFⓍAWAⓍFGA	KKKKKKAGFXAWAXFGA	KKKKKKAAFAAWAAFAA	17	S-6K-F17-2G	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		Lack of significant structure	Substitutions with polar, known 'helix-breaker' Gly residues led to losses in helical character until finally the peptide containing 3 Gly and 1 Asn (S-6K-F17-3GN) shows a severe loss in helical structure	Not found	Function: Antibacterial activity against Gram-negative bacteria. No experiments about antibacterial activity against Gram-positive bacteria are recorded.	[Ref.29275987] Gram-negative bacteria: E. coli (MIC= 1.6 μM)	[Ref.29275987] MHC = 15 μM against human red blood cells. Note: Minimum hemolytic concentration (MHC) is the minimum peptide concentration at which red blood cells undergo > 2% hemolysis.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 10 and 14) in sequence indicates 2-(4'-pentenyl) alanine. ②Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	29275987	Bioorg Med Chem. 2018 Mar 15;26(6):1189-1196. doi: 10.1016/j.bmc.2017.10.020. Epub 2017 Oct 21.	Tracy A Stone, Gregory B Cole, Huong Q Nguyen, Simon Sharpe, Charles M Deber	Influence of hydrocarbon-stapling on membrane interactions of synthetic antimicrobial peptides	Stapled AMP					
DRAMP21484	KKKKKKAGFⓍAWGⓍFGA	KKKKKKAGFXAWGXFGA	KKKKKKAAFAAWAAFAA	17	S-6K-F17-3G	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		Lack of significant structure	Substitutions with polar, known 'helix-breaker' Gly residues led to losses in helical character until finally the peptide containing 3 Gly and 1 Asn (S-6K-F17-3GN) shows a severe loss in helical structure	Not found	Function: Antibacterial activity against Gram-negative bacteria. No experiments about antibacterial activity against Gram-positive bacteria are recorded.	[Ref.29275987] Gram-negative bacteria: E. coli (MIC= 4.2 μM)	[Ref.29275987] MHC = 128 μM against human red blood cells. Note: Minimum hemolytic concentration (MHC) is the minimum peptide concentration at which red blood cells undergo > 2% hemolysis.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 10 and 14) in sequence indicates 2-(4'-pentenyl) alanine. ②Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	29275987	Bioorg Med Chem. 2018 Mar 15;26(6):1189-1196. doi: 10.1016/j.bmc.2017.10.020. Epub 2017 Oct 21.	Tracy A Stone, Gregory B Cole, Huong Q Nguyen, Simon Sharpe, Charles M Deber	Influence of hydrocarbon-stapling on membrane interactions of synthetic antimicrobial peptides	Stapled AMP					
DRAMP21485	KKKKKKNGFⓍAWGⓍFGA	KKKKKKNGFXAWGXFGA	KKKKKKAAFAAWAAFAA	17	S-6K-F17-3GN	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		Lack of significant structure	Substitutions with polar, known 'helix-breaker' Gly residues led to losses in helical character until finally the peptide containing 3 Gly and 1 Asn (S-6K-F17-3GN) shows a severe loss in helical structure	Not found	Function: Antibacterial activity against Gram-negative bacteria. No experiments about antibacterial activity against Gram-positive bacteria are recorded.	[Ref.29275987] Gram-negative bacteria: E. coli (MIC= 4.2 μM)	[Ref.29275987] MHC = 587 μM against human red blood cells. Note: Minimum hemolytic concentration (MHC) is the minimum peptide concentration at which red blood cells undergo > 2% hemolysis.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 10 and 14) in sequence indicates 2-(4'-pentenyl) alanine. ②Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	29275987	Bioorg Med Chem. 2018 Mar 15;26(6):1189-1196. doi: 10.1016/j.bmc.2017.10.020. Epub 2017 Oct 21.	Tracy A Stone, Gregory B Cole, Huong Q Nguyen, Simon Sharpe, Charles M Deber	Influence of hydrocarbon-stapling on membrane interactions of synthetic antimicrobial peptides	Stapled AMP					
DRAMP21486	VNWKKⓍLGKⓍIKVVK	VNWKKXLGKXIKVVK	VNWKKILGKIIKVVK	15	LL-IIIs-1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal		①20% α-helical content in water. ②55% α-helical content in 50% TFE. ③55% α-helical content in 8mM SDS.	It seems that the staple in the central part of the LL-IIIs-1 analog that crosslink the bend of the α-helix on its concave site around the Gly8 residue resulted in slight helix destabilization.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria and Antifungal activity against Candida albicans.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 0.7 μM), Bacillus subtilis (MIC = 0.8 μM), Staphylococcus aureus (MIC = 12.5 μM);##Gram-negative bacteria: E.coli (MIC = 4.4 μM), Pseudomonas aeruginosa (MIC = 78.7 μM);##Fungi: Candida albicans (MIC = 100 μM).	[Ref.22526241] LC50 = 31.3 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 6 and 10) indicates 2-(4'-pentenyl) alanine in the S configuration. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 18.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21487	VNWKKILGKⓍIKVⓍK	VNWKKILGKXIKVXK	VNWKKILGKIIKVVK	15	LL-IIIs-2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal		①31% α-helical content in water. ②51% α-helical content in 50% TFE. ③58% α-helical content in 8mM SDS.	For the LL-IIIs-2 analog, the helical content increment is slightly higher (8 %).	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria and Antifungal activity against Candida albicans.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 0.9 μM), Bacillus subtilis (MIC = 1.2 μM), Staphylococcus aureus (MIC = 20.3 μM);##Gram-negative bacteria: E.coli (MIC = 8.8 μM), Pseudomonas aeruginosa (MIC = 86.7 μM);##Fungi: Candida albicans (MIC = 100 μM).	[Ref.22526241] LC50 = 69 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 10 and 14) indicates 2-(4'-pentenyl) alanine in the S configuration. ②Ⓧ (10) and Ⓧ (14)are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 19.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21488	VⓍWKKⓍLGKIIKVVK	VXWKKXLGKIIKVVK	VNWKKILGKIIKVVK	15	LL-IIIs-3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal		①27% α-helical content in water. ②52% α-helical content in 50% TFE. ③62% α-helical content in 8mM SDS.	The CD spectra of the singly stapled peptides of the i, i + 4 type acquired in water show a slight increase (by 5 %) of helical content in the case of MEP-Ns-1, MEP-Ns-2 and LL-IIIs-3 compared to their unstapled precursors.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria and Antifungal activity against Candida albicans.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1.4 μM), Bacillus subtilis (MIC = 1.1 μM), Staphylococcus aureus (MIC = 21.7 μM);##Gram-negative bacteria: E.coli (MIC = 1.2 μM), Pseudomonas aeruginosa (MIC = 76.3 μM);##Fungi: Candida albicans (MIC = 93.3 μM).	[Ref.22526241] LC50 = 30 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) indicates 2-(4'-pentenyl) alanine in the S configuration. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 20.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21489	VNⓍKKIⓍGKⓍIKVⓍK	VNXKKIXGKXIKVXK	VNWKKILGKIIKVVK	15	LL-IIIs-4	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①45% α-helical content in water. ②48% α-helical content in 50% TFE. ③63% α-helical content in 8mM SDS.	On the other hand, the difference in α-helical content between doubly stapled analogs (LL-IIIs-4, MEP-Ns-3, MEP-Ns-5, and MEP-Ns-6) and their unstapled precursors is apparently higher, on average by 15 %	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 2 μM), Bacillus subtilis (MIC = 1.1 μM), Staphylococcus aureus (MIC = 80 μM);##Gram-negative bacteria: E.coli (MIC = 20 μM), Pseudomonas aeruginosa (MIC > 100 μM);##Fungi: Candida albicans (MIC > 100 μM).	[Ref.22526241] LC50 > 100 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 3, 7, 10 and 14) indicates 2-(4'-pentenyl) alanine in the S configuration. ②Ⓧ (3) and Ⓧ (7), Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 21.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21490	VNⓏKKILGKⓍIKVVK	VNZKKILGKXIKVVK	VNWKKILGKIIKVVK	15	LL-IIIs-5 cis	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①22% α-helical content in water. ②54% α-helical content in 50% TFE. ③69% α-helical content in 8mM SDS.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 0.9 μM), Bacillus subtilis (MIC = 0.9 μM), Staphylococcus aureus (MIC = 45 μM);##Gram-negative bacteria: E.coli (MIC = 3.5 μM), Pseudomonas aeruginosa (MIC = 80 μM);##Fungi: Candida albicans (MIC > 100 μM).	[Ref.22526241] LC50 = 100 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The Ⓩ (position: 3) indicates 2-(7'-octenyl) alanine in the R configuration. ②The Ⓧ (position: 10) is 2-(4'-pentenyl) alanine in the S configuration. ③Ⓩ (3) and Ⓧ (10) are cross-linked by hydrocarbon stapling.	L, cis	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 22.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21491	VNⓏKKILGKⓍIKVVK	VNZKKILGKXIKVVK	VNWKKILGKIIKVVK	15	LL-IIIs-5 trans	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①26% α-helical content in water. ②54% α-helical content in 50% TFE. ③62% α-helical content in 8mM SDS.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1.1 μM), Bacillus subtilis (MIC = 1.3 μM), Staphylococcus aureus (MIC = 65 μM);##Gram-negative bacteria: E.coli (MIC = 5.5 μM), Pseudomonas aeruginosa (MIC = 80 μM);##Fungi: Candida albicans (MIC > 100 μM).	[Ref.22526241] LC50 > 100 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The Ⓩ (position: 3) indicates 2-(7'-octenyl) alanine in the R configuration. ②The Ⓧ (position: 10) is 2-(4'-pentenyl) alanine in the S configuration. ③Ⓩ (3) and Ⓧ (10) are cross-linked by hydrocarbon stapling.	L, trans	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 23.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21492	VNⓍKKIⓍPKⓍIKVⓍK	VNXKKIXPKXIKVXK	VNWKKILGKIIKVVK	15	LL-IIIs-6a	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①36% α-helical content in water. ②42% α-helical content in 50% TFE. ③36% α-helical content in 8mM SDS.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1 μM), Bacillus subtilis (MIC = 1.1 μM), Staphylococcus aureus (MIC = 93 μM);##Gram-negative bacteria: E.coli (MIC = 7.8 μM), Pseudomonas aeruginosa (MIC = 93 μM);##Fungi: Candida albicans (MIC > 100 μM).	[Ref.22526241] LC50 > 100 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 3, 7, 10 and 14) indicates 2-(4'-pentenyl) alanine in the S configuration. ②Ⓧ (3) and Ⓧ (7), Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling respectively.	L, cis(around the S₅-Pro8 peptide bond)	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 24.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21493	VNⓍKKIⓍPKⓍIKVⓍK	VNXKKIXPKXIKVXK	VNWKKILGKIIKVVK	15	LL-IIIs-6b	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①33% α-helical content in water. ②38% α-helical content in 50% TFE. ③35% α-helical content in 8mM SDS.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 0.9 μM), Bacillus subtilis (MIC = 0.8 μM), Staphylococcus aureus (MIC = 50 μM);##Gram-negative bacteria: E.coli (MIC = 7.8 μM), Pseudomonas aeruginosa (MIC = 63 μM);##Fungi: Candida albicans (MIC > 100 μM).	[Ref.22526241] LC50 = 82μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 3, 7, 10 and 14) indicates 2-(4'-pentenyl) alanine in the S configuration. ②Ⓧ (3) and Ⓧ (7), Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling respectively.	L, trans(around the S₅-Pro8 peptide bond)	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 25.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21495	GFLSILKKVLPKⓍJAHⓍK	GFLSILKKVLPKXJAHXK	GFLSILKKVLPKVMAHMK	18	MEP-Ns-1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-, Antifungal		①19% α-helical content in water.②66% α-helical content in 50% TFE. ③75% α-helical content in 8mM SDS.	The CD spectra of the singly stapled peptides of the i, i + 4 type acquired in water show a slight increase (by 5 %) of helical content in the case of MEP-Ns-1, MEP-Ns-2 and LL-IIIs-3 compared to their unstapled precursors.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria and Antifungal activity against Candida albicans.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 0.8 μM), Bacillus subtilis (MIC = 1.1 μM), Staphylococcus aureus (MIC = 10.8 μM);##Gram-negative bacteria: E.coli (MIC = 2.5 μM), Pseudomonas aeruginosa (MIC = 77 μM);##Fungi: Candida albicans (MIC = 30 μM).	[Ref.22526241] LC50 = 18.1 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The J (position: 14) in sequence indicates norleucine. ②The Ⓧ (position: 13 and 17) indicates 2-(4'-pentenyl) alanine in the S configuration. ③Ⓧ (13) and Ⓧ (17) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 28.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21496	GFLSⓍLKKⓍLPKVJAHJK	GFLSXLKKXLPKVJAHJK	GFLSILKKVLPKVMAHMK	18	MEP-Ns-2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①16% α-helical content in water.②61% α-helical content in 50% TFE. ③62% α-helical content in 8mM SDS.	The CD spectra of the singly stapled peptides of the i, i + 4 type acquired in water show a slight increase (by 5 %) of helical content in the case of MEP-Ns-1, MEP-Ns-2 and LL-IIIs-3 compared to their unstapled precursors.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1.5 μM), Bacillus subtilis (MIC = 1.2 μM), Staphylococcus aureus (MIC = 37 μM);##Gram-negative bacteria: E.coli (MIC = 7.8 μM), Pseudomonas aeruginosa (MIC ≥ 100 μM);##Fungi: Candida albicans (MIC ≥ 100 μM).	[Ref.22526241] LC50 = 14.7 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The J (position: 14 and 17) in sequence indicates norleucine. ②The Ⓧ (position: 5 and 9) indicates 2-(4'-pentenyl) alanine in the S configuration. ③Ⓧ (5) and Ⓧ (9) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 29.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21497	GFLSⓍLKKⓍLPKⓍJAHⓍK	GFLSXLKKXLPKXJAHXK	GFLSILKKVLPKVMAHMK	18	MEP-Ns-3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①34% α-helical content in water.②56% α-helical content in 50% TFE. ③48% α-helical content in 8mM SDS.	On the other hand, the difference in α-helical content between doubly stapled analogs (LL-IIIs-4, MEP-Ns-3, MEP-Ns-5, and MEP-Ns-6) and their unstapled precursors is apparently higher, on average by 15 %	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC =  1.3 μM), Bacillus subtilis (MIC = 1.2 μM), Staphylococcus aureus (MIC ≥ 100 μM);##Gram-negative bacteria: E.coli (MIC = 46.7 μM), Pseudomonas aeruginosa (MIC > 100 μM);##Fungi: Candida albicans (MIC > 100 μM).	[Ref.22526241] LC50 = 13.9 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The J (position: 14) in sequence indicates norleucine. ②Ⓧ (position: 5, 9, 13 and 17) are 2-(4'-pentenyl) alanine in the S configuration. ③Ⓧ (5) and Ⓧ (9), Ⓧ (13) and Ⓧ (17) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 30.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21498	GFⓏSILKKVⓍPKVJAHJK	GFZSILKKVXPKVJAHJK	GFLSILKKVLPKVMAHMK	18	MEP-Ns-4 cis	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Unknown	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1.5 μM), Bacillus subtilis (MIC = 1.3 μM), Staphylococcus aureus (MIC = 37 μM);##Gram-negative bacteria: E.coli (MIC = 8.1 μM), Pseudomonas aeruginosa (MIC ≥ 100 μM);##Fungi: Candida albicans (MIC ≥ 100 μM).	[Ref.22526241] LC50 = 11 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The J (position: 14 and 17) in sequence indicates norleucine. ②Ⓧ (position: 10) is 2-(4'-pentenyl) alanine in the S configuration. ③Ⓩ (position: 3) is 2-(7'-pctenyl) lalnine in the R configuration. ④Ⓧ (10) and Ⓩ (3) are cross-linked by hydrocarbon stapling.	L, cis	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 31.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21499	GFⓏSILKKVⓍPKVJAHJK	GFZSILKKVXPKVJAHJK	GFLSILKKVLPKVMAHMK	18	MEP-Ns-4 trans	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Unknown	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 2 μM), Bacillus subtilis (MIC = 1.7 μM), Staphylococcus aureus (MIC = 63 μM);##Gram-negative bacteria: E.coli (MIC = 16.3 μM), Pseudomonas aeruginosa (MIC ≥ 100 μM);##Fungi: Candida albicans (MIC ≥ 100 μM).	[Ref.22526241] LC50 = 20 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The J (position: 14 and 17) in sequence indicates norleucine. ②Ⓧ (position: 10) is 2-(4'-pentenyl) alanine in the S configuration. ③Ⓩ (position: 3) is 2-(7'-pctenyl) lalnine in the R configuration. ④Ⓧ (10) and Ⓩ (3) are cross-linked by hydrocarbon stapling.	L, trans	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 32.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21500	GFLSⓍLKKⓍLGKⓍJAHⓍK	GFLSXLKKXLGKXJAHXK	GFLSILKKVLPKVMAHMK	18	MEP-Ns-5	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①52% α-helical content in water.②67% α-helical content in 50% TFE. ③59% α-helical content in 8mM SDS.	On the other hand, the difference in α-helical content between doubly stapled analogs (LL-IIIs-4, MEP-Ns-3, MEP-Ns-5, and MEP-Ns-6) and their unstapled precursors is apparently higher, on average by 15 %	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1.5 μM), Bacillus subtilis (MIC = 1.2 μM), Staphylococcus aureus (MIC = 57 μM);##Gram-negative bacteria: E.coli (MIC = 5.6 μM), Pseudomonas aeruginosa (MIC > 100 μM);##Fungi: Candida albicans (MIC > 100 μM).	[Ref.22526241] LC50 = 29 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The J (position: 14) in sequence indicates norleucine. ②Ⓧ (position: 5, 9, 13 and 17) indicates 2-(4'-pentenyl) alanine in the S configuration. ③Ⓧ (5) and Ⓧ (9), Ⓧ (13) and Ⓧ (17) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 33.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21501	GFLSⓍLKKⓍLAKⓍJAHⓍK	GFLSXLKKXLAKXJAHXK	GFLSILKKVLPKVMAHMK	18	MEP-Ns-6	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①56% α-helical content in water.②64% α-helical content in 50% TFE. ③56% α-helical content in 8mM SDS.	On the other hand, the difference in α-helical content between doubly stapled analogs (LL-IIIs-4, MEP-Ns-3, MEP-Ns-5, and MEP-Ns-6) and their unstapled precursors is apparently higher, on average by 15 %	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 100 μM.	[Ref.22526241] Gram-positive bacteria: Micrococcus luteus (MIC = 1.5 μM), Bacillus subtilis (MIC = 1.2 μM), Staphylococcus aureus (MIC = 43 μM);##Gram-negative bacteria: E.coli (MIC = 5.6 μM), Pseudomonas aeruginosa (MIC > 100 μM);##Fungi: Candida albicans (MIC > 100 μM).	[Ref.22526241] LC50 = 39.5 μM. Note: LC50 is the concentration of a peptide able to lyse 50% of human erthrocytes in the assay.	Cyclic (Stapled)	Free	Amidation	①The J (position: 14) in sequence indicates norleucine. ②Ⓧ (position: 5, 9, 13 and 17) indicates 2-(4'-pentenyl) alanine in the S configuration. ③Ⓧ (5) and Ⓧ (9), Ⓧ (13) and Ⓧ (17) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	22526241	Amino Acids. 2012 Nov;43(5):2047-58. doi: 10.1007/s00726-012-1283-1. Epub 2012 Apr 34.	Hubert Chapuis, Jiřina Slaninová, Lucie Bednárová, Lenka Monincová, Miloš Buděšínský, Václav Čeřovský	Effect of hydrocarbon stapling on the properties of α-helical antimicrobial peptides isolated from the venom of hymenoptera	Stapled AMP					
DRAMP21502	IDWKKLLⓀAAKⒼIL	IDWKKLLKAAKGIL	IDWKKLLDAAKQIL	14	C-MP1-1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①Slight α-helical structure in aqueous solution. ②Increased α-helical conformation in 30 mM SDS and 50% TFE compared with MPI	①By CD, we observed that C-MPI-2 and MPI did not display any structural preferences in aqueous solution, whereas C-MPI-1 adopoted a slight α-helical structure. ②C-MPI-1 also had higher α-helicity than MPI in membrane mimicking environments, including 30 mM sodium dodecyl sulfate (SDS) and 50% trifluoroethyl alcohol (TFE).	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.28833783] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 64 μM), Bacillus subtilis ATCC 23857 (MIC = 8 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 64 μM), Pseudomonas aeruginosa ATCC 27853 (No antimicrobial activity)	[Ref.28833783] It has 0%, 0%, 2.1%, 9.5%, 22.7%, 25.4% and 34.7% against human red blood cells at peptide concentrations of 0, 5, 10, 25, 50, 75 and 150 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓚ (position: 8) is lysine with the methyltrityl side chain. ②The Ⓖ (position: 12) is propargylglycine. ③Ⓚ (8) and Ⓖ (12) are cross-linked by hydrocarbon stapling by 1,3-diploar azide-alkyne cyclization.	L	No cytotoxicity information found in the reference	28833783	J Pept Sci. 2017 Nov;23(11):824-832. doi: 10.1002/psc.3031. Epub 2017 Aug 23.	Beijun Liu, Wei Zhang, Sanhu Gou, Haifeng Huang, Jia Yao, Zhibin Yang, Hui Liu, Chao Zhong, Beiyin Liu, Jingman Ni, Rui Wang	Intramolecular cyclization of the antimicrobial peptide Polybia-MPI with triazole stapling: influence on stability and bioactivity	Stapled AMP					
DRAMP21503	IⓀWKKLLⒼAAKQIL	IKWKKLLGAAKQIL	IDWKKLLDAAKQIL	14	C-MP1-2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No structural preference in aqueous solution.	By CD, we observed that C-MPI-2 and MPI did not display any structural preferences in aqueous solution, whereas C-MPI-1 adopoted a slight α-helical structure.	Not found	Function: Antibacterial activity against Gram-positive bacteria. Antibacterial activity against Gram-negative bacteria is not so evident.	[Ref.28833783] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 256 μM), Bacillus subtilis ATCC 23857 (MIC = 128 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 128 μM), Pseudomonas aeruginosa ATCC 27853 (No antimicrobial activity)	[Ref.28833783] No hemolytic information found.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓚ (position: 2) is lysine with the methyltrityl side chain. ②The Ⓖ (position: 8) is propargylglycine. ③Ⓚ (2) and Ⓖ (8) are cross-linked by hydrocarbon stapling by 1,3-diploar azide-alkyne cyclization.	L	No cytotoxicity information found in the reference	28833783	J Pept Sci. 2017 Nov;23(11):824-832. doi: 10.1002/psc.3031. Epub 2017 Aug 23.	Beijun Liu, Wei Zhang, Sanhu Gou, Haifeng Huang, Jia Yao, Zhibin Yang, Hui Liu, Chao Zhong, Beiyin Liu, Jingman Ni, Rui Wang	Intramolecular cyclization of the antimicrobial peptide Polybia-MPI with triazole stapling: influence on stability and bioactivity	Stapled AMP					
DRAMP21504	ⓍIGKⓍLHSAKKFGKAFVGEIJNS	XIGKXLHSAKKFGKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)0	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		15% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 59.46 μg/mL), Bacillus cereus ATCC 14579 (MIC = 10.49 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 25.00 μg/mL)	[Ref.31427820] It has 5.6% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 1 and 5) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (1) and Ⓧ (5) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21505	GⓍGKFⓍHSAKKFGKAFVGEIJNS	GXGKFXHSAKKFGKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		12% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 100.00 μg/mL), Bacillus cereus ATCC 14579 (MIC = 14.87 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.72 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 12.50 μg/mL)	[Ref.31427820] It has 4.6% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21506	GIⓍKFLⓍSAKKFGKAFVGEIJNS	GIXKFLXSAKKFGKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		21% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 12.50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 7.43 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 10.51 μg/mL)	[Ref.31427820] It has 18.3% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 3 and 7) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (3) and Ⓧ (7) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21507	GIGⓍFLHⓍAKKFGKAFVGEIJNS	GIGXFLHXAKKFGKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		18% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 8.84 μg/mL), Bacillus cereus ATCC 14579 (MIC = 10.51 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 7.43 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 25.00 μg/mL)	[Ref.31427820] It has 42.1% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 4 and 8) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (4) and Ⓧ (8) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21508	GIGKⓍLHSⓍKKFGKAFVGEIJNS	GIGKXLHSXKKFGKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)4	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		24% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 25.00 μg/mL), Bacillus cereus ATCC 14579 (MIC = 6.25 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 4.42 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 10.51 μg/mL)	[Ref.31427820] It has 18.3% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 5 and 9) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (5) and Ⓧ (9) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21509	GIGKFⓍHSAⓍKFGKAFVGEIJNS	GIGKFXHSAXKFGKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)5	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		14% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 25.00 μg/mL), Bacillus cereus ATCC 14579 (MIC = 17.68 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 14.87 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 50.00 μg/mL)	[Ref.31427820] It has 13.2% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 6 and 10) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21510	GIGKFLⓍSAKⓍFGKAFVGEIJNS	GIGKFLXSAKXFGKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)6	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		18% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 25.00 μg/mL), Bacillus cereus ATCC 14579 (MIC = 12.50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 7.43 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 29.73 μg/mL)	[Ref.31427820] It has 7.6% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 7 and 11) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (7) and Ⓧ (11) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21511	GIGKFLHⓍAKKⓍGKAFVGEIJNS	GIGKFLHXAKKXGKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)7	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		26% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 6.25 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 6.25 μg/mL)	[Ref.31427820] It has 62.4% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 8 and 12) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (8) and Ⓧ (12) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21512	GIGKFLHSⓍKKFⓍKAFVGEIJNS	GIGKFLHSXKKFXKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)8	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		49% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 7.44 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.26 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.72 μg/mL)	[Ref.31427820] It has 74.6% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 9 and 13) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21513	GIGKFLHSAⓍKFGⓍAFVGEIJNS	GIGKFLHSAXKFGXAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)9	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		28% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 17.68 μg/mL), Bacillus cereus ATCC 14579 (MIC = 12.50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 4.42 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 59.46 μg/mL)	[Ref.31427820] It has 60.0% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 10 and 14) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (10) and Ⓧ (14) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21514	GIGKFLHSAKⓍFGKⓍFVGEIJNS	GIGKFLHSAKXFGKXFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)10	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		15% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 8.84 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.42 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 4.42 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 14.87 μg/mL)	[Ref.31427820] It has 23.9% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 11 and 15) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (11) and Ⓧ (15) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21515	GIGKFLHSAKKⓍGKAⓍVGEIJNS	GIGKFLHSAKKXGKAXVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)11	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		16% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 12.50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.26 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 8.84 μg/mL)	[Ref.31427820] It has 17.8% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 12 and 16) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (12) and Ⓧ (16) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21516	GIGKFLHSAKKFⓍKAFⓍGEIJNS	GIGKFLHSAKKFXKAFXGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)12	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		51% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 7.43 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 4.42 μg/mL)	[Ref.31427820] It has 76.6% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 13 and 17) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (13) and Ⓧ (17) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21517	GIGKFLHSAKKFGⓍAFVⓍEIJNS	GIGKFLHSAKKFGXAFVXEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)13	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		23% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 5.26 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 7.43 μg/mL)	[Ref.31427820] It has 60.0% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 14 and 18) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (14) and Ⓧ (18) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21518	GIGKFLHSAKKFGKⓍFVGⓍIJNS	GIGKFLHSAKKFGKXFVGXIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)14	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		19% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 2.63 μg/mL), Bacillus cereus ATCC 14579 (MIC = 2.63 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.86 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.13 μg/mL)	[Ref.31427820] It has 92.4% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 15 and 19) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (15) and Ⓧ (19) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21519	GIGKFLHSAKKFGKAⓍVGEⓍJNS	GIGKFLHSAKKFGKAXVGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)15	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		15% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 10.51 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.26 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 7.43 μg/mL)	[Ref.31427820] It has 11.7% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21520	GIGKFLHSAKKFGKAFⓍGEIⓍNS	GIGKFLHSAKKFGKAFXGEIXNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)16	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		11% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 42.04 μg/mL), Bacillus cereus ATCC 14579 (MIC = 7.44 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.72 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 8.84 μg/mL)	[Ref.31427820] It has 8.1% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 17 and 21) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②Ⓧ (17) and Ⓧ (21) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21521	GIGKFLHSAKKFGKAFVⓍEIJⓍS	GIGKFLHSAKKFGKAFVXEIJXS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)17	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		30% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 2.63 μg/mL), Bacillus cereus ATCC 14579 (MIC = 1.86 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.56 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 5.26 μg/mL)	[Ref.31427820] It has 56.3% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 18 and 22) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (18) and Ⓧ (22) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21522	GIGKFLHSAKKFGKAFVGⓍIJNⓍ	GIGKFLHSAKKFGKAFVGXIJNX	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(I+4)18	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		19% α-helical content in 25-50 μM potassium phosphate solution.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 1.56 μg/mL), Bacillus cereus ATCC 14579 (MIC = 2.21 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.56 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 2.21 μg/mL)	[Ref.31427820] It has 101.0% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 19 and 23) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (19) and Ⓧ (23) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21523	KIGKFLHSAKKFGKAⓍVGEⓍJNS	KIGKFLHSAKKFGKAXVGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(G1K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 21.02 μg/mL), Bacillus cereus ATCC 14579 (MIC = 10.49 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 5.41 μg/mL)	[Ref.31427820] It has 4.4% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21524	GKGKFLHSAKKFGKAⓍVGEⓍJNS	GKGKFLHSAKKFGKAXVGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(I2K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 35.36 μg/mL), Bacillus cereus ATCC 14579 (MIC = 25.00 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 4.75 μg/mL)	[Ref.31427820] It has 2.2% hemolysis against human red blood cells at 25 μg/mL..	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21525	GIKKFLHSAKKFGKAⓍVGEⓍJNS	GIKKFLHSAKKFGKAXVGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(G3K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 10.49 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.21 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.34 μg/mL)	[Ref.31427820] It has 3.7% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21526	GIGKKLHSAKKFGKAⓍVGEⓍJNS	GIGKKLHSAKKFGKAXVGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(F5K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 50 μg/mL.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC > 50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.69 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 17.68 μg/mL)	[Ref.31427820] It has 1.5% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21527	GIGKFKHSAKKFGKAⓍVGEⓍJNS	GIGKFKHSAKKFGKAXVGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(L6K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 50 μg/mL.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC > 50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 17.68 μg/mL)	[Ref.31427820] It has 1.7% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21528	GIGKFLKSAKKFGKAⓍVGEⓍJNS	GIGKFLKSAKKFGKAXVGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(H7K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 6.20 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.21 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 2.69 μg/mL)	[Ref.31427820] It has 3.1% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21529	GIGKFLHKAKKFGKAⓍVGEⓍJNS	GIGKFLHKAKKFGKAXVGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(S8K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 6.20 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.38 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 2.65 μg/mL)	[Ref.31427820] It has 3.3% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21530	GIGKFLHSKKKFGKAⓍVGEⓍJNS	GIGKFLHSKKKFGKAXVGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(A9K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 50 μg/mL.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC > 50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 4.38 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 15.81 μg/mL)	[Ref.31427820] It has 1.7% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21531	GIGKFLHSAKKKGKAⓍVGEⓍJNS	GIGKFLHSAKKKGKAXVGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(F12K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 50 μg/mL.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC > 50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 14.03 μg/mL)	[Ref.31427820] It has 1.9% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21532	GIGKFLHSAKKFKKAⓍVGEⓍJNS	GIGKFLHSAKKFKKAXVGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(G13K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 50 μg/mL.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC > 50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.10 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 12.82 μg/mL)	[Ref.31427820] It has 1.8% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21533	GIGKFLHSAKKFGKKⓍVGEⓍJNS	GIGKFLHSAKKFGKKXVGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(A15K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 31.50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 9.89 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.10 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 4.97 μg/mL)	[Ref.31427820] It has 6.5% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21534	GIGKFLHSAKKFGKAⓍKGEⓍJNS	GIGKFLHSAKKFGKAXKGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(V17K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC > 50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.91 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 19.84 μg/mL)	[Ref.31427820] It has 2.1% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21535	GIGKFLHSAKKFGKAⓍVKEⓍJNS	GIGKFLHSAKKFGKAXVKEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(G18K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 8.80 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.38 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.23 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.57 μg/mL)	[Ref.31427820] It has 8.3% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21536	GIGKFLHSAKKFGKAⓍVGKⓍJNS	GIGKFLHSAKKFGKAXVGKXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(E19K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 25.00 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.21 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 2.23 μg/mL)	[Ref.31427820] It has 6.0% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21537	GIGKFLHSAKKFGKAⓍVGEⓍKNS	GIGKFLHSAKKFGKAXVGEXKNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(B21K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC > 50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 29.73 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.23 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 8.97 μg/mL)	[Ref.31427820] It has 3.2% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21538	GIGKFLHSAKKFGKAⓍVGEⓍJKS	GIGKFLHSAKKFGKAXVGEXJKS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(N22K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 21.02 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.38 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.60 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.57 μg/mL)	[Ref.31427820] It has 11.9% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21539	GIGKFLHSAKKFGKAⓍVGEⓍJNK	GIGKFLHSAKKFGKAXVGEXJNK	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)15(S23K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 42.04 μg/mL), Bacillus cereus ATCC 14579 (MIC = 7.39 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.23 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.57 μg/mL)	[Ref.31427820] It has 4.9% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The J (position: 21) in sequence is norlercine. ③Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21540	GⓍGSFⓍKKKAHVGKHⓍGKAⓍLTHYL	GXGSFXKKKAHVGKHXGKAXLTHYL	GWGSFFKKAAHVGKHVGKAALTHYL	25	Pleu(i+4)1,15(A9K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 3.13 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.13 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.56 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 1.56 μg/mL)	[Ref.31427820] It has 17.1% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2, 6, 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21541	GⓍRKRⓍRKFRNKIKEKKKKIGQKⓍQGLⓍPKLA	GXRKRXRKFRNKIKEKKKKIGQKXQGLXPKLA	GLRKRLRKFRNKIKEKLKKIGQKIQGFVPKLAPRTDY	32	CAP(i+4)1,23(L17K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 12.5 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.13 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.56 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 1.56 μg/mL)	[Ref.31427820] It has 4.0% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2, 6, 24 and 28) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (24) and X (28) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21542	GⓍGKFⓍHSKKKFGKAⓍVGEⓍBNS	GXGKFXHSKKKFGKAXVGEXBNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+4)1,15(A9K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 6.25 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.56 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.12 μg/mL)	[Ref.31427820] It has 1.9% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2, 6, 16 and 20) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The B (position: 21) in sequence is norlercine. ③Ⓧ (2) and Ⓧ (6), Ⓧ (16) and Ⓧ (20) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21543	GⓍFSKⓍKGKKIKNLⓍISGⓍKG	GXFSKXKGKKIKNLXISGXKG	GIFSKLAGKKIKNLLISGLK	21	Esc(i+4)1,14(A7K)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 12.5 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.56 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 1.56 μg/mL)	[Ref.31427820] It has 2.5% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2, 6, 15 and 19) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (15) and Ⓧ (19) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21544	GⓏGKFLHSⓍKKFGKAFVGEIJNS	GZGKFLHSXKKFGKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 10.51 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.42 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.63 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.72 μg/mL)	[Ref.31427820] It has 49.7% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 9) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 2) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (9) and Ⓩ (2) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21545	GIⓏKFLHSAⓍKFGKAFVGEIJNS	GIZKFLHSAXKFGKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 12.50 μg/mL), Bacillus cereus ATCC 14579 (MIC = 8.84 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 4.42 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 21.02 μg/mL)	[Ref.31427820] It has 66.1% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 10) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 3) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (10) and Ⓩ (3) are cross-linked by hydrocarbon stapling. 	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21546	GIGⓏFLHSAKⓍFGKAFVGEIJNS	GIGZFLHSAKXFGKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 14.87 μg/mL), Bacillus cereus ATCC 14579 (MIC = 12.50 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 10.51 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 21.02 μg/mL)	[Ref.31427820] It has 52.6% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 11) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 4) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (11) and Ⓩ (4) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21547	GIGKⓏLHSAKKⓍGKAFVGEIJNS	GIGKZLHSAKKXGKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)4	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 21.02 μg/mL), Bacillus cereus ATCC 14579 (MIC = 5.26 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.21 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 6.25 μg/mL)	[Ref.31427820] It has 42.5% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 12) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 5) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (12) and Ⓩ (5) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21548	GIGKFⓏHSAKKFⓍKAFVGEIJNS	GIGKFZHSAKKFXKAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)5	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 8.84 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.13 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.13 μg/mL)	[Ref.31427820] It has 82.9% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 13) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 6) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (13) and Ⓩ (6) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21549	GIGKFLⓏSAKKFGⓍAFVGEIJNS	GIGKFLZSAKKFGXAFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)6	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 6.25 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.42 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 14.87 μg/mL)	[Ref.31427820] It has 45.3% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 14) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 7) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (14) and Ⓩ (7) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21550	GIGKFLHⓏAKKFGKⓍFVGEIJNS	GIGKFLHZAKKFGKXFVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)7	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 3.72 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 5.26 μg/mL)	[Ref.31427820] It has 95.3% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 15) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 8) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (15) and Ⓩ (8) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21551	GIGKFLHSⓏKKFGKAⓍVGEIJNS	GIGKFLHSZKKFGKAXVGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)8	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 6.25 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.21 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.72 μg/mL)	[Ref.31427820] It has 85.2% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 16) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 9) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (16) and Ⓩ (9) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21552	GIGKFLHSAⓏKFGKAFⓍGEIJNS	GIGKFLHSAZKFGKAFXGEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)9	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 3.72 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.42 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.72 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 5.26 μg/mL)	[Ref.31427820] It has 94.6% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 17) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 10) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (17) and Ⓩ (10) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21553	GIGKFLHSAKⓏFGKAFVⓍEIJNS	GIGKFLHSAKZFGKAFVXEIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)10	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 4.42 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.13 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 7.43 μg/mL)	[Ref.31427820] It has 43.8% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 18) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 11) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (18) and Ⓩ (11) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21554	GIGKFLHSAKKⓏGKAFVGⓍIJNS	GIGKFLHSAKKZGKAFVGXIJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)11	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 2.63 μg/mL), Bacillus cereus ATCC 14579 (MIC = 2.63 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.21 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 2.21 μg/mL)	[Ref.31427820] It has 84.7% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 19) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 12) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (19) and Ⓩ (12) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21555	GIGKFLHSAKKFⓏKAFVGEⓍJNS	GIGKFLHSAKKFZKAFVGEXJNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)12	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 7.44 μg/mL), Bacillus cereus ATCC 14579 (MIC = 3.72 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.21 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.13 μg/mL)	[Ref.31427820] It has 75.4% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 20) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 13) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (20) and Ⓩ (13) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21556	GIGKFLHSAKKFGⓏAFVGEIⓍNS	GIGKFLHSAKKFGZAFVGEIXNS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)13	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 7.44 μg/mL), Bacillus cereus ATCC 14579 (MIC = 4.42 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.21 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 6.25 μg/mL)	[Ref.31427820] It has 63.5% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 21) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 14) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③Ⓧ (21) and Ⓩ (14) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21557	GIGKFLHSAKKFGKⓏFVGEIJⓍS	GIGKFLHSAKKFGKZFVGEIJXS	GIGKFLHSAKKFGKAFVGEIMNS	23	Mag(i+7)14	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in potassium phosphate buffer.	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.31427820] Gram-positive bacteria: Staphyolococcus aureus ATCC 25923 (MIC = 2.63 μg/mL), Bacillus cereus ATCC 14579 (MIC = 1.56 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 1.86 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 5.26 μg/mL)	[Ref.31427820] It has 68.1% hemolysis against human red blood cells at 25 μg/mL.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 22) in sequence is S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓩ (position: 15) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ③The J (position: 21) in sequence is norlercine. ④Ⓧ (22) and Ⓩ (15) are cross-linked by hydrocarbon stapling.	L	No cytotoxicity information found in the reference	31427820	Nat Biotechnol. 2019 Oct;37(10):1186-1197. doi: 10.1038/s41587-019-0222-z. Epub 2019 Aug 19.	Rida Mourtada, Henry D Herce, Daniel J Yin, Jamie A Moroco, Thomas E Wales, John R Engen, Loren D Walensky	Design of stapled antimicrobial peptides that are stable, nontoxic and kill antibiotic-resistant bacteria in mice	Stapled AMP					
DRAMP21558	KⓍWKJⓍK	KXWKJXK	/	7	KKK	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate solution (pH 6.5).	①[Ref.30361948] All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices. ②[Ref.28547390] Peptide NLE and LYS maintained a compatible helicity to LEU.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphyolococcus aureus ATCC 6538p (MIC = 6.3 μM),  Staphylococcus epidermidis ATCC 12228 (MIC= 25 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysenteriae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 50 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 18.8 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM).##[Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 6.3 μg/mL), Staphylococcus aureus (MIC = 6.3 μg/mL), Staphylococcus epidermidis (MIC = 12.5 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 12.5 μg/mL), Shigella dysenteriae (MIC = 12.5 μg/mL), Salmonella typhimurium (MIC = 50 μg/mL), Klebsiella pneumoniae (MIC = 18.8 μg/mL), Pseudomonas aeruginosa (MIC = 12.5 μg/mL).	[Ref.30361948] It has 1.5% hemolysis against human red blood cells at 25 μM and 3.0% hemolysis at 50 μM.##[Ref.28547390] 3.3% hemolysis against human red blood cells at 25 μM and 7.9% hemolysis t 50 μM.	Cyclic (Stapled)	Free	Amidation	①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30361948##28547390	Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25.##Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim. ##Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim	Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides.##Mono-substitution effects on antimicrobial activity of stapled heptapeptides.	Stapled AMP					
DRAMP21559	RⓍWKJⓍK	RXWKJXK	/	7	RKK	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate solution (pH 6.5).	All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 6.3 μM), Staphyolococcus aureus ATCC 6538p (MIC = 6.3 μM),  Staphylococcus epidermidis ATCC 12228 (MIC= 12.5 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysenteriae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 25 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 12.5 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM)	[Ref.30361948] It has 1.5% hemolysis against human red blood cells at 25 μM and 4.3% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30361948	Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides	Stapled AMP					
DRAMP21560	KⓍWRJⓍK	KXWRJXK	/	7	KRK	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate solution (pH 6.5).	All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphyolococcus aureus ATCC 6538p (MIC = 6.3 μM),  Staphylococcus epidermidis ATCC 12228 (MIC= 25 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysenteriae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 75 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 37.5 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM)	[Ref.30361948] It has <1% hemolysis against human red blood cells at 25 μM and 2.0% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30361948	Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides	Stapled AMP					
DRAMP21561	KⓍWKJⓍR	KXWKJXR	/	7	KKR	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate solution (pH 6.5).	All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 9.4 μM), Staphyolococcus aureus ATCC 6538p (MIC = 6.3 μM),  Staphylococcus epidermidis ATCC 12228 (MIC = 25 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysenteriae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 50 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 12.5 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM)	[Ref.30361948] It has 1.4% hemolysis against human red blood cells at 25 μM and % hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30361948	Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides	Stapled AMP					
DRAMP21562	RⓍWRJⓍK	RXWRJXK	/	7	RRK	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate solution (pH 6.5).	All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphyolococcus aureus ATCC 6538p (MIC = 6.3 μM),  Staphylococcus epidermidis ATCC 12228 (MIC = 25 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysenteriae ATCC 9752 (MIC = 37.5 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 50 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 37.5 μM)	[Ref.30361948] It has <1% hemolysis against human red blood cells at 25 μM and <1% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30361948	Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides	Stapled AMP					
DRAMP21563	RⓍWKJⓍR	RXWKJXR	/	7	RKR	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate solution (pH 6.5).	All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 6.3 μM), Staphyolococcus aureus ATCC 6538p (MIC = 3.2 μM),  Staphylococcus epidermidis ATCC 12228 (MIC = 12.5 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 6.3 μM), Shigella dysenteriae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 25 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 18.8 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM)	[Ref.30361948] It has 8.2% hemolysis against human red blood cells at 25 μM and 17.0% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30361948	Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides	Stapled AMP					
DRAMP21564	KⓍWRJⓍR	KXWRJXR	/	7	KRR	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate solution (pH 6.5).	All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 6.3 μM), Staphyolococcus aureus ATCC 6538p (MIC = 3.2 μM),  Staphylococcus epidermidis ATCC 12228 (MIC = 12.5 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysenteriae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 50 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 18.8 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 37.5 μM)	[Ref.30361948] It has 1.8% hemolysis against human red blood cells at 25 μM and 5.6% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30361948	Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides	Stapled AMP					
DRAMP21565	RⓍWRJⓍR	RXWRJXR	/	7	RRR	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate solution (pH 6.5).	All analogs displayed similar CD spectra to that of KKK, having two minima near 208 and 222 nm and a maximum near 190nm, which are characteristic of α-helices.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.30361948] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphyolococcus aureus ATCC 6538p (MIC = 6.3 μM),  Staphylococcus epidermidis ATCC 12228 (MIC = 25 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysenteriae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumoniae ATCC 10031 (MIC = 50 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 100 μM)	[Ref.30361948] It has <1% hemolysis against human red blood cells at 25 μM and 3.2% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The J (position: 5) in sequence is norleucine. ②The Ⓧ (position: 2 and 6) indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30361948	Arch Pharm Res. 2018 Nov;41(11):1092-1097. doi: 10.1007/s12272-018-1084-5. Epub 2018 Oct 25.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Effects of lysine-to-arginine substitution on antimicrobial activity of cationic stapled heptapeptides	Stapled AMP					
DRAMP21566	IDWKKⓍLDAⓍKQIL	IDWKKXLDAXKQIL	IDWKKLLDAAKQIL	14	MP1S	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+		96.1% α-helical content in a 25 mM potassium phosphate buffer solution at 20℃	①Helical contents of all stapled analogues were increased by more than a three-fold compared to their unmodified counterpart, MP1. ②MP1S appears to be the most helical among this panel of peptides, showing 96% helicity, which is 3.7-fold higher than that of MP1.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.29075946] Gram-positive bacteria: Bacillus subtilis (MIC = 1.6 μg/mL), Staphylococcus aureus (MIC = 2.4 μg/mL), Staphylococcus epidermidis (MIC > 100 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC > 100 μg/mL), Shigella dysenteriae (MIC > 100 μg/mL), Salmonella typhimurium (MIC > 100 μg/mL), Klebsiella pneumoniae (MIC > 100 μg/mL), Pseudomonas aeruginosa (MIC > 100 μg/mL)	[Ref.29075946] It has 6.7% hemolysis against human red blood cells at 12.5 μM and 12.3% hemolysis at 25 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 6 and 10) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	29075946	Arch Pharm Res. 2017 Dec;40(12):1414-1419. doi: 10.1007/s12272-017-0963-5. Epub 2017 Oct 26.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim 	Antimicrobial activity and stability of stapled helices of polybia-MP1	Stapled AMP					
DRAMP21567	IDWKKⓍLNAⓍKQIL	IDWKKXLNAXKQIL	IDWKKLLDAAKQIL	14	MP1S-D8N	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		89.4% α-helical content in a 25 mM potassium phosphate buffer solution at 20℃	①Helical contents of all stapled analogues were increased by more than a three-fold compared to their unmodified counterpart, MP1. ②MP1S-D8N and MP1S-Q12K showed slightly lower helical contents (89.4% and 81.8, respectively).	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.29075946] Gram-positive bacteria: Bacillus subtilis (MIC = 0.8 μg/mL), Staphylococcus aureus (MIC = 0.8 μg/mL), Staphylococcus epidermidis (MIC = 37.5 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 50 μg/mL), Shigella dysenteriae (MIC = 100 μg/mL), Salmonella typhimurium (MIC > 100 μg/mL), Klebsiella pneumoniae (MIC = 37.5 μg/mL), Pseudomonas aeruginosa (MIC ≥ 100 μg/mL)	[Ref.29075946] It has 16.3% hemolysis against human red blood cells at 12.5 μM and 37.8% hemolysis at 25 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 6 and 10) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	29075946	Arch Pharm Res. 2017 Dec;40(12):1414-1419. doi: 10.1007/s12272-017-0963-5. Epub 2017 Oct 26.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim 	Antimicrobial activity and stability of stapled helices of polybia-MP1	Stapled AMP					
DRAMP21568	IDWKKⓍLDAⓍKKIL	IDWKKXLDAXKKIL	IDWKKLLDAAKQIL	14	MP1S-Q12K	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+		81.8% α-helical content in a 25 mM potassium phosphate buffer solution at 20℃	①Helical contents of all stapled analogues were increased by more than a three-fold compared to their unmodified counterpart, MP1. ②MP1S-D8N and MP1S-Q12K showed slightly lower helical contents (89.4% and 81.8, respectively).	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.29075946] Gram-positive bacteria: Bacillus subtilis (MIC = 0.8 μg/mL), Staphylococcus aureus (MIC = 1.2 μg/mL), Staphylococcus epidermidis (MIC = 50 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC >100  μg/mL), Shigella dysenteriae (MIC > 100 μg/mL), Salmonella typhimurium (MIC > 100 μg/mL), Klebsiella pneumoniae (MIC > 100 μg/mL), Pseudomonas aeruginosa (MIC > 100 μg/mL)	[Ref.29075946] It has 9.6% hemolysis against human red blood cells at 12.5 μM and 13.9% hemolysis at 25 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 6 and 10) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	29075946	Arch Pharm Res. 2017 Dec;40(12):1414-1419. doi: 10.1007/s12272-017-0963-5. Epub 2017 Oct 26.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim 	Antimicrobial activity and stability of stapled helices of polybia-MP1	Stapled AMP					
DRAMP21569	KⓍWKAⓍK	KXWKAXK	/	7	ALA	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 25 μg/mL), Staphylococcus aureus (MIC = 25 μg/mL), Staphylococcus epidermidis (MIC = 100 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 25 μg/mL), Shigella dysenteriae (MIC = 50 μg/mL), Salmonella typhimurium (MIC = 100 μg/mL), Klebsiella pneumoniae (MIC = 25 μg/mL), Pseudomonas aeruginosa (MIC = 25 μg/mL)	[Ref.28547390] It has <1% hemolysis against human red blood cells at 25 μM and <1% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	28547390	Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25.	Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim	Mono-substitution effects on antimicrobial activity of stapled heptapeptides	Stapled AMP					
DRAMP21570	KⓍWKLⓍK	KXWKLXK	/	7	LEU	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 25 μg/mL), Staphylococcus aureus (MIC = 12.5 μg/mL), Staphylococcus epidermidis (MIC = 50 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 12.5 μg/mL), Shigella dysenteriae (MIC = 25 μg/mL), Salmonella typhimurium (MIC = 50 μg/mL), Klebsiella pneumoniae (MIC = 18.8 μg/mL), Pseudomonas aeruginosa (MIC = 18.8 μg/mL)	[Ref.28547390] It has <1% hemolysis against human red blood cells at 25 μM and <1% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	28547390	Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25.	Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim	Mono-substitution effects on antimicrobial activity of stapled heptapeptides	Stapled AMP					
DRAMP21571	KⓍWKVⓍK	KXWKVXK	/	7	VAL	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃	Compared to LEU, the previously reported stapled heptapeptide containing leucine in position 5, peptides VAL and ILE, carrying valine and isoleucine, respectively, appeared slightly more helical as determined by the CD signal intensity at 222 nm.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 18.8 μg/mL), Staphylococcus aureus (MIC = 12.5 μg/mL), Staphylococcus epidermidis (MIC = 50 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 18.8 μg/mL), Shigella dysenteriae (MIC = 25 μg/mL), Salmonella typhimurium (MIC = 100 μg/mL), Klebsiella pneumoniae (MIC = 18.8 μg/mL), Pseudomonas aeruginosa (MIC = 12.5 μg/mL)	[Ref.28547390] It has 1.3% hemolysis against human red blood cells at 25 μM and 2.5% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	28547390	Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25.	Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim	Mono-substitution effects on antimicrobial activity of stapled heptapeptides	Stapled AMP					
DRAMP21572	KⓍWKIⓍK	KXWKIXK	/	7	ILE	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃	Compared to LEU, the previously reported stapled heptapeptide containing leucine in position 5, peptides VAL and ILE, carrying valine and isoleucine, respectively, appeared slightly more helical as determined by the CD signal intensity at 222 nm.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 25 μg/mL), Staphylococcus aureus (MIC = 25 μg/mL), Staphylococcus epidermidis (MIC = 50 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 25 μg/mL), Shigella dysenteriae (MIC = 25 μg/mL), Salmonella typhimurium (MIC = 100 μg/mL), Klebsiella pneumoniae (MIC = 37.5 μg/mL), Pseudomonas aeruginosa (MIC = 12.5 μg/mL)	[Ref.28547390] It has 1.5% hemolysis against human red blood cells at 25 μM and 3.0% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	28547390	Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25.	Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim	Mono-substitution effects on antimicrobial activity of stapled heptapeptides	Stapled AMP					
DRAMP21573	KⓍWKFⓍK	KXWKFXK	/	7	PHE	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃	On the other hand, peptide PHE, TRP, and GLU, bearing phenylalanine, tryptophan, and glutamate, respectively, showed a slightly decreased helicity.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 25 μg/mL), Staphylococcus aureus (MIC = 25 μg/mL), Staphylococcus epidermidis (MIC = 50 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 25 μg/mL), Shigella dysenteriae (MIC = 50 μg/mL), Salmonella typhimurium (MIC = 100 μg/mL), Klebsiella pneumoniae (MIC = 25 μg/mL), Pseudomonas aeruginosa (MIC = 12.5 μg/mL)	[Ref.28547390] It has 1.9% hemolysis against human red blood cells at 25 μM and 4.4% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	28547390	Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25.	Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim	Mono-substitution effects on antimicrobial activity of stapled heptapeptides	Stapled AMP					
DRAMP21574	KⓍWKWⓍK	KXWKWXK	/	7	TRP	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃	On the other hand, peptide PHE, TRP, and GLU, bearing phenylalanine, tryptophan, and glutamate, respectively, showed a slightly decreased helicity.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 12.5 μg/mL), Staphylococcus aureus (MIC = 12.5 μg/mL), Staphylococcus epidermidis (MIC = 25 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 12.5 μg/mL), Shigella dysenteriae (MIC = 25 μg/mL), Salmonella typhimurium (MIC = 50 μg/mL), Klebsiella pneumoniae (MIC = 12.5 μg/mL), Pseudomonas aeruginosa (MIC = 12.5 μg/mL)	[Ref.28547390] It has 3.2% hemolysis against human red blood cells at 25 μM and 5.9% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	28547390	Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25.	Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim	Mono-substitution effects on antimicrobial activity of stapled heptapeptides	Stapled AMP					
DRAMP21575	KⓍWKEⓍK	KXWKEXK	/	7	GLU	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃	On the other hand, peptide PHE, TRP, and GLU, bearing phenylalanine, tryptophan, and glutamate, respectively, showed a slightly decreased helicity.	Not found	Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 100 μg/mL.	[Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC > 100 μg/mL), Staphylococcus aureus (MIC > 100 μg/mL), Staphylococcus epidermidis (MIC > 100 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 37.5 μg/mL), Shigella dysenteriae (MIC > 100 μg/mL), Salmonella typhimurium (MIC > 100 μg/mL), Klebsiella pneumoniae (MIC = 50 μg/mL), Pseudomonas aeruginosa (MIC = 25 μg/mL)	[Ref.28547390] It has <1% hemolysis against human red blood cells at 25 μM and <1% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	28547390	Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25.	Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim	Mono-substitution effects on antimicrobial activity of stapled heptapeptides	Stapled AMP					
DRAMP21576	KⓍWKKⓍK	KXWKKXK	/	7	LYS	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃	Peptide NLE and LYS maintained a compatible helicity to LEU.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.28547390] Gram-positive bacteria: Bacillus subtilis (MIC = 100 μg/mL), Staphylococcus aureus (MIC = 50 μg/mL), Staphylococcus epidermidis (MIC > 100 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 50 μg/mL), Shigella dysenteriae (MIC = 100 μg/mL), Salmonella typhimurium (MIC > 100 μg/mL), Klebsiella pneumoniae (MIC = 75 μg/mL), Pseudomonas aeruginosa (MIC = 6.3 μg/mL)	[Ref.28547390] It has <1% hemolysis against human red blood cells at 25 μM and <1% hemolysis at 50 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	28547390	Arch Pharm Res. 2017 Jun;40(6):713-719. doi: 10.1007/s12272-017-0922-1. Epub 2017 May 25.	Huy X Luong, Do-Hee Kim, Ngoan T Mai, Bong-Jin Lee, Young-Woo Kim	Mono-substitution effects on antimicrobial activity of stapled heptapeptides	Stapled AMP					
DRAMP21578	WWVⓍARAⓍRR	WWVXARAXRR	WWVXARAXRR	10	Val-HSLP	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		0.0% α-helix and 34.3% β-strand in 20 mM potassium phosphate buffer; 0.8% α-helix and 36.9% β-strand in 20 mM potassium phosphate buffer made 30% in TFE.	Only slghtly higher β-strand characteristics were observed for the hydrocarbon-stapled peptides in 30% TFE. Overall, the peptides showed some β-strand secondary structural characteristics and little α-helical content.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. Antifungal activity against Candida albicans is not noteable under 20 μM.	[Ref.28073163] Gram-positive bacteria: Bacillus megaterium ATCC 14581 (IC50 = 0.69 μM, MIC = 5.00 μM), Staphylococcus aureus ATCC 6538 (IC50 = 0.63 μM, MIC = 5.00 μM), Enterococcus faecalis ATCC 29212 (IC50 = 0.65 μM, MIC = 5.00 μM);##Gram-negative bacteria: Escherichia coli ATCC 700926 (IC50 = 14.8 μM, MIC > 20 μM);## Fungi: Candida albicans 002 ATCC 64385 (IC50 > 20 μM, MIC > 20 μM), C. albicans 004 ATCC MYA-2876 (IC50 > 20 μM, MIC > 20 μM).	[Ref.28073163] HC50 = 14.5 μM against human red blood cells. Note:  HC50 is the half-maximal hemolytic concentration.	Cyclic (Stapled)	Acylation (Valerylamide)	Amidation	①The Ⓧ (position: 4 and 8) in sequence indicates (S)-2-(4'-pentenyl)-alanine. ②Ⓧ (4) and Ⓧ (8) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	28073163	Biopolymers. 2017 May;108(3). doi: 10.1002/bip.23006.	Zachary B Jenner, Christopher M Crittenden, Martín Gonzalez, Jennifer S Brodbelt, Kerry A Bruns	Hydrocarbon-stapled lipopeptides exhibit selective antimicrobial activity	Stapled AMP					
DRAMP21580	WWVⓍAFAⓍRRR	WWVXAFAXRRR	WWVXAFAXRRR	11	Cap-HSLP	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+		0.3% α-helix and 31.9% β-strand in 20 mM potassium phosphate buffer; 0.7% α-helix and 40.0% β-strand in 20 mM potassium phosphate buffer made 30% in TFE.	Only slghtly higher β-strand characteristics were observed for the hydrocarbon-stapled peptides in 30% TFE. Overall, the peptides showed some β-strand secondary structural characteristics and little α-helical content.	Not found	Function: Antibacterial activity against Gram-positive bacteria. Antibacterial activity against Gram-negative bacteria and antifungal activity against Candida albicans are not noteable under 20 μM.	[Ref.28073163] Gram-positive bacteria: Bacillus megaterium ATCC 14581 (IC50 = 1.63 μM, MIC = 5.00 μM), Staphylococcus aureus ATCC 6538 (IC50 = 0.64 μM, MIC = 5.00 μM), Enterococcus faecalis ATCC 29212 (IC50 = 0.63 μM, MIC = 1.25 μM);##Gram-negative bacteria: Escherichia coli ATCC 700926 (IC50 = 169 μM, MIC > 20 μM);## Fungi: Candida albicans 002 ATCC 64385 (IC50 > 20 μM, MIC > 20 μM), C. albicans 004 ATCC MYA-2876 (IC50 > 20 μM, MIC > 20 μM).	[Ref.28073163] HC50 = 4.49 μM against human red blood cells. Note:  HC50 is the half-maximal hemolytic concentration.	Cyclic (Stapled)	Acylation (Caproylamide)	Amidation	①The Ⓧ (position: 4 and 8) in sequence indicates (S)-2-(4'-pentenyl)-alanine. ②Ⓧ (4) and Ⓧ (8) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	28073163	Biopolymers. 2017 May;108(3). doi: 10.1002/bip.23006.	Zachary B Jenner, Christopher M Crittenden, Martín Gonzalez, Jennifer S Brodbelt, Kerry A Bruns	Hydrocarbon-stapled lipopeptides exhibit selective antimicrobial activity	Stapled AMP					
DRAMP21582	KⓍWKLⓍK	KXWKLXK	/	7	S3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		Stable α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. 	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μg/mL), Staphylcocccus aureus ATCC 6538p (MIC = 25 μg/mL), Staphylcococcus epidermis ATCC 12228 (MIC = 100 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μg/mL), Shigella dysentariae ATCC 9752 (MIC = 50 μg/mL), Salmonella typhimurium ATCC 14028 (MIC = 100 μg/mL), Klebsiella pneumonia ATCC 10031 (MIC = 50 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 75 μg/mL)	It has <1% hemolysis against human red blood cells at 6.3 μM and <1% hemolysis at 12.5 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2016 Aug;37(8)1199-1203. doi: 10.1002/bkcs.10839.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Antimicrobial and Hemolytic Activity of Stapled Heptapeptide Dimers	Stapled AMP					
DRAMP21583	KⓍWKLⓍKGKⓍWKLⓍK	KXWKLXKGKXWKLXK	/	15	3GL3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃	3GL3 apperaed to be the most helical in this series as indicated by the distinct double minima near at 208 and 222 nm with similar intensities.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. 	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 2.3 μg/mL), Staphylcocccus aureus ATCC 6538p (MIC = 1.6 μg/mL), Staphylcococcus epidermis ATCC 12228 (MIC = 3.1 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.1 μg/mL), Shigella dysentariae ATCC 9752 (MIC = 37.5 μg/mL), Salmonella typhimurium ATCC 14028 (MIC = 50 μg/mL), Klebsiella pneumonia ATCC 10031 (MIC = 6.3 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 9.4 μg/mL)	It has 19.1% hemolysis against human red blood cells at 6.3 μM and 31.8% hemolysis at 12.5 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2, 6, 10 and 14) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (10) and Ⓧ (14) are cross-linked respectively by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2016 Aug;37(8)1199-1203. doi: 10.1002/bkcs.10839.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Antimicrobial and Hemolytic Activity of Stapled Heptapeptide Dimers	Stapled AMP					
DRAMP21584	KⓍWKLⓍKAKⓍWKLⓍK	KXWKLXKAKXWKLXK	/	15	3BA3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. 	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 2.3 μg/mL), Staphylcocccus aureus ATCC 6538p (MIC = 1.6 μg/mL), Staphylcococcus epidermis ATCC 12228 (MIC = 3.1 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.1 μg/mL), Shigella dysentariae ATCC 9752 (MIC = 6.3 μg/mL), Salmonella typhimurium ATCC 14028 (MIC = 12.5 μg/mL), Klebsiella pneumonia ATCC 10031 (MIC = 3.1 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 3.1 μg/mL)	It has 14.2% hemolysis against human red blood cells at 6.3 μM and 24.9% hemolysis at 12.5 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The A (position: 8) in sequence is β-Ala. ②The Ⓧ (position: 2, 6, 10 and 14) in sequence indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6), Ⓧ (10) and Ⓧ (14) are cross-linked respectively by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2016 Aug;37(8)1199-1203. doi: 10.1002/bkcs.10839.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Antimicrobial and Hemolytic Activity of Stapled Heptapeptide Dimers	Stapled AMP					
DRAMP21585	KⓍWKLⓍKBKⓍWKLⓍK	KXWKLXKBKXWKLXK	/	15	3GA3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. 	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 4.0 μg/mL), Staphylcocccus aureus ATCC 6538p (MIC = 3.1 μg/mL), Staphylcococcus epidermis ATCC 12228 (MIC = 12.5 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 4.7 μg/mL), Shigella dysentariae ATCC 9752 (MIC = 18.8 μg/mL), Salmonella typhimurium ATCC 14028 (MIC = 37.5 μg/mL), Klebsiella pneumonia ATCC 10031 (MIC = 9.4 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 9.4 μg/mL)	It has 12.9% hemolysis against human red blood cells at 6.3 μM and 24.4% hemolysis at 12.5 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The B (position: 8) in sequence is γ-aminobutyric acid (GABA). ②The Ⓧ (position: 2, 6, 10 and 14) in sequence indicates (S)-α-methyl, α-pentenylglycine. ③Ⓧ (2) and Ⓧ (6), Ⓧ (10) and Ⓧ (14) are cross-linked respectively by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2016 Aug;37(8)1199-1203. doi: 10.1002/bkcs.10839.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Antimicrobial and Hemolytic Activity of Stapled Heptapeptide Dimers	Stapled AMP					
DRAMP21586	KⓍWKLⓍKPKⓍWKLⓍK	KXWKLXKPKXWKLXK	/	15	3PR3-X	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃	"Whereas all other
dimeric analogs were obtained as a single exclusive product,
the proline-containing sequence yielded two products
(3PR3-X and 3PR3-Y) in similar amounts. These might be
conformational isomers induced by the cis–trans configuration
of the proline linker. 3PR3-X, which showed the weakest hemolytic activity, displayed a CD spectrum similar to that of the monomeric S3 and the lowest helical content in this series."	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria. 	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 3.1 μg/mL), Staphylcocccus aureus ATCC 6538p (MIC = 4.7 μg/mL), Staphylcococcus epidermis ATCC 12228 (MIC = 12.5 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.1 μg/mL), Shigella dysentariae ATCC 9752 (MIC = 6.3 μg/mL), Salmonella typhimurium ATCC 14028 (MIC = 12.5 μg/mL), Klebsiella pneumonia ATCC 10031 (MIC = 4.7 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 9.4 μg/mL)	It has 5.7% hemolysis against human red blood cells at 6.3 μM and 9.7% hemolysis at 12.5 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2, 6, 10 and 14) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (10) and Ⓧ (14) are cross-linked respectively by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. 	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2016 Aug;37(8)1199-1203. doi: 10.1002/bkcs.10839.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Antimicrobial and Hemolytic Activity of Stapled Heptapeptide Dimers	Stapled AMP					
DRAMP21587	KⓍWKLⓍKPKⓍWKLⓍK	KXWKLXKPKXWKLXK	/	15	3PR3-Y	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution at 20 ℃	"Whereas all other
dimeric analogs were obtained as a single exclusive product,
the proline-containing sequence yielded two products
(3PR3-X and 3PR3-Y) in similar amounts. These might be
conformational isomers induced by the cis–trans configuration
of the proline linker."	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 1.2 μg/mL), Staphylcocccus aureus ATCC 6538p (MIC = 1.2 μg/mL), Staphylcococcus epidermis ATCC 12228 (MIC = 4.7 μg/mL);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 2.3 μg/mL), Shigella dysentariae ATCC 9752 (MIC = 4.7 μg/mL), Salmonella typhimurium ATCC 14028 (MIC = 12.5 μg/mL), Klebsiella pneumonia ATCC 10031 (MIC = 3.1 μg/mL), Pseudomonas aeruginosa ATCC 27853 (MIC = 6.3 μg/mL)	It has 16.2% hemolysis against human red blood cells at 6.3 μM and 31.9% hemolysis at 12.5 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2, 6, 10 and 14) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (10) and Ⓧ (14) are cross-linked respectively by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple. ③The P (position: 8) in sequence is D-proline.	Mixed (D-Pro8)	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2016 Aug;37(8)1199-1203. doi: 10.1002/bkcs.10839.	Huy X Luong, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Antimicrobial and Hemolytic Activity of Stapled Heptapeptide Dimers	Stapled AMP					
DRAMP21588	KⓍWKAⓍK	KXWKAXK	/	7	Ac-S1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution (pH 6.5)	On the contraty, conformations of sequences S1 and S3 appear to be not greatly affected by the presence of the N-acetyl cap.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μM), Staphylococcus aureus ATCC 6538p (MIC = 25 μM), Staphylcocccus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 37.5 μM), Shigella dysentariae ATCC 9752 (MIC = 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 50 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 50 μM)	It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0% and <1.0% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483.	Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim	N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities	Stapled AMP					
DRAMP21589	KⓍWKAⓍK	KXWKAXK	/	7	H-S1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution (pH 6.5)	On the contraty, conformations of sequences S1 and S3 appear to be not greatly affected by the presence of the N-acetyl cap.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μM), Staphylococcus aureus ATCC 6538p (MIC = 37.5 μM), Staphylcocccus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 100 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 25 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 25 μM)	It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0% and <1.0% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483.	Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim	N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities	Stapled AMP					
DRAMP21590	KⓍAKWⓍK	KXAKWXK	/	7	Ac-S2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution (pH 6.5)	Conformational analysis using far ultraviolet CD spectrometry indicates that the removal of the N-acetyl cap from Ac-S2 and Ac-S4 causes a significant loss of their helical contents.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μM), Staphylococcus aureus ATCC 6538p (MIC = 37.5 μM), Staphylcocccus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 37.5 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 50 μM)	It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0% and <1.0% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483.	Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim	N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities	Stapled AMP					
DRAMP21591	KⓍAKWⓍK	KXAKWXK	/	7	H-S2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix (but less helical than Ac-S2) in a 25 mM potassium phosphate buffer solution (pH 6.5)	Conformational analysis using far ultraviolet CD spectrometry indicates that the removal of the N-acetyl cap from Ac-S2 and Ac-S4 causes a significant loss of their helical contents.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 37.5 μM), Staphylococcus aureus ATCC 6538p (MIC = 37.5 μM), Staphylcocccus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 50 μM), Shigella dysentariae ATCC 9752 (MIC > 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 50 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 37.5 μM)	It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0% and <1.0% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483.	Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim	N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities	Stapled AMP					
DRAMP21592	KⓍWKLⓍK	KXWKLXK	/	7	Ac-S3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution (pH 6.5)	On the contraty, conformations of sequences S1 and S3 appear to be not greatly affected by the presence of the N-acetyl cap.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 18.8 μM), Staphylococcus aureus ATCC 6538p (MIC = 25 μM), Staphylcocccus epidermis ATCC 12228 (MIC = 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 75 μM), Klebsiella pneumonia ATCC 10031 (MIC = 37.5 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 50 μM)	It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, 1.59% and 5.53% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483.	Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim	N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities	Stapled AMP					
DRAMP21593	KⓍWKLⓍK	KXWKLXK	/	7	H-S3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution (pH 6.5)	①On the contraty, conformations of sequences S1 and S3 appear to be not greatly affected by the presence of the N-acetyl cap. ②It should be also noted that H-S3 displays a CD spectrum that is typically observed from α-helical peptides even without the N-acetyl cal.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphylococcus aureus ATCC 6538p (MIC = 12.5 μM), Staphylcocccus epidermis ATCC 12228 (MIC = 25 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysentariae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 50 μM), Klebsiella pneumonia ATCC 10031 (MIC = 25 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 25 μM)	It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0% and 2.05% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483.	Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim	N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities	Stapled AMP					
DRAMP21594	KⓍLKWⓍK	KXLKWXK	/	7	Ac-S4	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in a 25 mM potassium phosphate buffer solution (pH 6.5)	Conformational analysis using far ultraviolet CD spectrometry indicates that the removal of the N-acetyl cap from Ac-S2 and Ac-S4 causes a significant loss of their helical contents.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphylococcus aureus ATCC 6538p (MIC = 25 μM), Staphylcocccus epidermis ATCC 12228 (MIC = 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 37.5 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 50 μM)	It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, 1.29%, 2.84% and 5.74% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483.	Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim	N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities	Stapled AMP					
DRAMP21595	KⓍLKWⓍK	KXLKWXK	/	7	H-S4	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix (but less helix content Ac-S4) in a 25 mM potassium phosphate buffer solution (pH 6.5)	Conformational analysis using far ultraviolet CD spectrometry indicates that the removal of the N-acetyl cap from Ac-S2 and Ac-S4 causes a significant loss of their helical contents.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μM), Staphylococcus aureus ATCC 6538p (MIC = 50 μM), Staphylcocccus epidermis ATCC 12228 (MIC = 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 75 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 37.5 μM), Pseudomonas aeruginose ATCC 27853 (MIC = 25 μM)	It has <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0%, <1.0% and <1.0% hemolysis against human red blood cells at 0.8,1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pententylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC. 2015 Oct;36(10)2511-2515. doi: 10.1002/bkcs.10483.	Thuy T.T. Dinh, Do-Hee Kim, Thang Q. Nguyen, Bong-Jin Lee, Young-Woo Kim	N-Capping Effects of Stapled Heptapeptides on Antimicrobial and Hemolytic Activities	Stapled AMP					
DRAMP21597	KⓍAKAⓍKKAAKAAWK	KXAKAXKKAAKAAWK	KAAKAAKKAAKAAWK	15	Ac-SS-14W	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 25 mM potassium phosphate buffer solution (pH6.5)	On the other hand, singly-stapled analog Ac-SS-14W exhibited a typical CD spectrum for α-helix, characterized by two minima near 208 and 222 nm and a maximum near 190 nm. 	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μM), Staphylococcus aureus ATCC 6538p (MIC = 9.4 μM), Staphylococcus epidermis ATCC 12228 (MIC = 75 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.1 μM), Shigella dysentariae ATCC 9752 (MIC = 18.8 μM), Salmonella typhimurium ATCC 14028 (MIC > 200 μM), Klebsiella pneumonia ATCC 10031 (MIC = 18.8 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 100 μM).	[Ref.26235946] It has <1% hemolysis against human red blood cells at 12.5 μM and <1% hemolysis at 25 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	26235946	Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19.	Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim	Antimicrobial activity of doubly-stapled alanine/lysine-based peptides	Stapled AMP					
DRAMP21598	KⓍAKAⓍKKⓍAKAⓍWK	KXAKAXKKXAKAXWK	KAAKAAKKAAKAAWK	15	Ac-DS-14W	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 25 mM potassium phosphate buffer solution (pH6.5)	Doubly-stapled Ac-Ds-14W showed the most enhaced helical contents among this series of peptides. 	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 1.6 μM), Staphylococcus aureus ATCC 6538p (MIC = 4.8 μM), Staphylococcus epidermis ATCC 12228 (MIC = 3.1 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 200 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 100 μM).	[Ref.26235946] It has 22.1% hemolysis against human red blood cells at 12.5 μM and 38.8% hemolysis at 25 μM. 	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 ,6, 9 and 13) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	26235946	Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19.	Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim	Antimicrobial activity of doubly-stapled alanine/lysine-based peptides	Stapled AMP					
DRAMP21599	KⓍAKAⓍKKⓍAKWⓍAK	KXAKAXKKXAKWXAK	KAAKAAKKAAKAAWK	15	Ac-DS-12W	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 25 mM potassium phosphate buffer solution (pH 6.5)	In addition, the positional modification of tryptophan caused significant changes in the conformation: in the CD analysis, the most active Ac-DS-5W exhibited markedly enhanced helical content whereas the equipotent Ac-DS-12W showed decreased helicity compared to Ac-DS-14W	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 1.6 μM), Staphylococcus aureus ATCC 6538p (MIC = 3.1 μM), Staphylococcus epidermis ATCC 12228 (MIC = 4.8 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 50 μM), Shigella dysentariae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 200 μM), Klebsiella pneumonia ATCC 10031 (MIC = 100 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 100 μM).	[Ref.26235946] It has 25.3% hemolysis against human red blood cells at 12.5 μM and 42.5% hemolysis at 25 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 ,6, 9 and 13) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	26235946	Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19.	Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim	Antimicrobial activity of doubly-stapled alanine/lysine-based peptides	Stapled AMP					
DRAMP21600	KⓍAKWⓍKKⓍAKAⓍAK	KXAKWXKKXAKAXAK	KAAKAAKKAAKAAWK	15	Ac-DS-3W	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 25 mM potassium phosphate buffer solution (pH 6.5)	No other descriptive information about the structure found in the literature	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 3.1 μM), Staphylococcus aureus ATCC 6538p (MIC = 4.8 μM), Staphylococcus epidermis ATCC 12228 (MIC = 4.8 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC = 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 100 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 200 μM).	[Ref.26235946] It has 28.9% hemolysis against human red blood cells at 12.5 μM and 42.5% hemolysis at 25 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 ,6, 9 and 13) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	26235946	Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19.	Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim	Antimicrobial activity of doubly-stapled alanine/lysine-based peptides	Stapled AMP					
DRAMP21601	KⓍWKAⓍKKⓍAKAⓍAK	KXWKAXKKXAKAXAK	KAAKAAKKAAKAAWK	15	Ac-DS-5W	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 25 mM potassium phosphate buffer solution (pH 6.5)	In addition, the positional modification of tryptophan caused significant changes in the conformation: in the CD analysis, the most active Ac-DS-5W exhibited markedly enhanced helical content whereas the equipotent Ac-DS-12W showed decreased helicity compared to Ac-DS-14W	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 1.6 μM), Staphylococcus aureus ATCC 6538p (MIC = 1.6 μM), Staphylococcus epidermis ATCC 12228 (MIC = 1.6 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 18.8 μM), Shigella dysentariae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 37.5 μM), Klebsiella pneumonia ATCC 10031 (MIC = 37.5 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM).	[Ref.26235946] It has 22.0% hemolysis against human red blood cells at 12.5 μM and 38.5% hemolysis at 25 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 ,6, 9 and 13) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	26235946	Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19.	Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim	Antimicrobial activity of doubly-stapled alanine/lysine-based peptides	Stapled AMP					
DRAMP21602	KⓍAKWⓍKKⓍAKAⓍAK	KXAKWXKKXAKAXAK	KAAKAAKKAAKAAWK	15	Su-DS-5W	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 25 mM potassium phosphate buffer solution (pH 6.5)	In the CD experiments, these new analogs showed similar helicity to Ac-DS-5W although Su-DS-5W displayed a slight increase in helicity whereas H-DS-5W exhibited a slight decrease.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 1.6 μM), Staphylococcus aureus ATCC 6538p (MIC = 1.6 μM), Staphylococcus epidermis ATCC 12228 (MIC = 1.6 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysentariae ATCC 9752 (MIC = 50 μM), Salmonella typhimurium ATCC 14028 (MIC > 200 μM), Klebsiella pneumonia ATCC 10031 (MIC = 25 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 50 μM).	[Ref.26235946] It has 19.5% hemolysis against human red blood cells at 12.5 μM and 32.6% hemolysis at 25 μM.	Cyclic (Stapled)	Succinylation	Amidation	①The Ⓧ (position: 2 ,6, 9 and 13) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	26235946	Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19.	Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim	Antimicrobial activity of doubly-stapled alanine/lysine-based peptides	Stapled AMP					
DRAMP21603	KⓍAKWⓍKKⓍAKAⓍAK	KXAKWXKKXAKAXAK	KAAKAAKKAAKAAWK	15	H-DS-5W	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 25 mM potassium phosphate buffer solution (pH 6.5)	In the CD experiments, these new analogs showed similar helicity to Ac-DS-5W although Su-DS-5W displayed a slight increase in helicity whereas H-DS-5W exhibited a slight decrease.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.26235946] Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 1.6 μM), Staphylococcus aureus ATCC 6538p (MIC = 1.6 μM), Staphylococcus epidermis ATCC 12228 (MIC = 1.6 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 3.1 μM), Shigella dysentariae ATCC 9752 (MIC = 12.5 μM), Salmonella typhimurium ATCC 14028 (MIC = 6.3 μM), Klebsiella pneumonia ATCC 10031 (MIC = 12.5 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 6.3 μM).	[Ref.26235946] It has 13.5% hemolysis against human red blood cells at 12.5 μM and 25.5% hemolysis at 25 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 ,6, 9 and 13) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (2) and Ⓧ (6), Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	26235946	Bioorg Med Chem Lett. 2015 Sep 15;25(18):4016-9. doi: 10.1016/j.bmcl.2015.06.053. Epub 2015 Jun 19.	Thuy T T Dinh, Do-Hee Kim, Huy X Luong, Bong-Jin Lee, Young-Woo Kim	Antimicrobial activity of doubly-stapled alanine/lysine-based peptides	Stapled AMP					
DRAMP21605	KⓍWKAⓍK	KXWKAXK	KXWKAXK	7	S1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 25 mM potassium phosphate buffer solution (pH 6.5)	As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 25 μM), Staphylococcus aureus ATCC 6538p (MIC = 25 μM), Staphylococcus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 50 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 50 μM).	It has 0.53%, 0.53%, 0.67%, 0.66%, 0.83%, 1.36%, 1.17% and 1.21% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC	Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim	De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides	Stapled AMP					
DRAMP21607	KⓍAKWⓍK	KXAKWXK	KXAKWXK	7	S2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 25 mM potassium phosphate buffer solution (pH 6.5)	As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 50 μM), Staphylococcus aureus ATCC 6538p (MIC = 100 μM), Staphylococcus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 50 μM), Shigella dysentariae ATCC 9752 (MIC > 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 100 μM), Pseudomonas aeruginosa ATCC 27853 (MIC > 100 μM).	It has 0.91%, 1.01%, 0.66%, 0.99%, 0.63%, 0.82%, 1.75% and 1.24% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC	Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim	De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides	Stapled AMP					
DRAMP21609	KⓍWKLⓍK	KXWKLXK	KXWKLXK	7	S3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 25 mM potassium phosphate buffer solution (pH 6.5)	As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphylococcus aureus ATCC 6538p (MIC = 25 μM), Staphylococcus epidermis ATCC 12228 (MIC = 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 12.5 μM), Shigella dysentariae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 50 μM), Klebsiella pneumonia ATCC 10031 (MIC = 25 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM).	It has 0.87%, 0.65%, 1.02%, 0.94%, 2.13%, 2.52%, 3.81% and 7.75% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC	Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim	De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides	Stapled AMP					
DRAMP21611	KⓍLKWⓍK	KXLKWXK	KXLKWXK	7	S4	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 25 mM potassium phosphate buffer solution (pH 6.5)	As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 12.5 μM), Staphylococcus aureus ATCC 6538p (MIC = 12.5 μM), Staphylococcus epidermis ATCC 12228 (MIC = 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 25 μM), Shigella dysentariae ATCC 9752 (MIC = 25 μM), Salmonella typhimurium ATCC 14028 (MIC = 50 μM), Klebsiella pneumonia ATCC 10031 (MIC = 25 μM), Pseudomonas aeruginosa ATCC 27853 (MIC = 25 μM).	It has 0.71%, 0.90%, 0.71%, 0.68%, 1.08%, 1.87%, 2.46% and 4.50% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC	Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim	De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides	Stapled AMP					
DRAMP21613	KⓍWAKⓍA	KXWAKXA	KXWAKXA	7	S5	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 25 mM potassium phosphate buffer solution (pH 6.5)	As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC = 50 μM), Staphylococcus aureus ATCC 6538p (MIC = 50 μM), Staphylococcus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 50 μM), Shigella dysentariae ATCC 9752 (MIC > 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC = 100 μM), Pseudomonas aeruginosa ATCC 27853 (MIC > 100 μM).	It has 0.65%, 0.60%, 0.59%, 0.76%, 0.83%, 0.76%, 0.88% and 1.04% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC	Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim	De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides	Stapled AMP					
DRAMP21615	KⓍAWKⓍA	KXAWKXA	KXAWKXA	7	S6	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		α-helix in 25 mM potassium phosphate buffer solution (pH 6.5)	As expected, in the far ultraviolet circular dichroism (CD) experiment, the stapled heptapeptides displayed enhanced helical contents compared to their corresponding unstapled counterparts.	Not found	Function: Antibacterial activity against Gram-negative bacteria. Antibacterial activity against Gram-positive bacteria is not noteable under 100 μM.	Gram-positive bacteria: Bacillus subtilis ATCC 6633 (MIC > 100 μM), Staphylococcus aureus ATCC 6538p (MIC > 100 μM), Staphylococcus epidermis ATCC 12228 (MIC > 100 μM);##Gram-negative bacteria: Escherichia coli ATCC 25922 (MIC = 100 μM), Shigella dysentariae ATCC 9752 (MIC > 100 μM), Salmonella typhimurium ATCC 14028 (MIC > 100 μM), Klebsiella pneumonia ATCC 10031 (MIC > 100 μM), Pseudomonas aeruginosa ATCC 27853 (MIC > 100 μM).	It has 0.65%, 0.66%, 0.75%, 0.87%, 0.64%, 0.70%, 0.61% and 0.84% hemolysis against human red blood cells at 0.8, 1.6, 3.1, 6.3, 12.5, 25.0, 50.0 and 100.0 μM.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates (S)-α-methyl, α-pentenylglycine. Note: the Experimental section presenst that X is (S)-α-methyl, α-pentenylglycine, while the Results section presents that X is pentenylalanine. We incline to the former representation according to previous papers published by the research group which the author belonged. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found.	PubMed ID is not available	B KOREAN CHEM SOC	Thuy T.T. Dinh, Do-Hee Kim, Song-Jin Lee, Young-Woo Kim	De Novo Design and Their Antimicrobial Activity of Stapled Amphipathic Helices of Heptapeptides	Stapled AMP					
DRAMP21617	qqrkrkiwsⓚlapⓓgttlvklvagig	qqrkrkiwsklapdgttlvklvagig	qqrkrkiwsilaplgttlvklvagig	26	sDRIM	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①Disordered (or unstructured) conformation in aqueous solutions [pure water (H₂O), phosphate buffer (PB, 10 mM), and phosphate buffer with high salt (NaF, 100 mM)]. ②50% average α-helix content in various membranes environments [57% in POPC; 67% in POPC/P	In the cases of sDRIM and sKFGF, stapling did not induce any conformational change, as these analogues remained just as unstructured.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.28921993] Gram-positive bacteria: Staphylococcus aureus (MIC = 128 μg/mL), Enterococcus faecalis (MIC = 64 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 32 μg/mL), Pseudomonas aeruginosa (MIC = 64 μg/mL)	[Ref.28921993] It has 13.4%, 18.6%, 25.4%, 33.4%, 42.0%, 46.9%, 42.2% and 47.1% hemolysis against human red blood cells at 5, 7.5, 10, 15, 20, 25, 30 and 40 μg/ml.	Cyclic (Stapled)	Free	Amidation	ⓚ (10) and ⓓ (14) are corss-linked by lactam stapling through the polar amide bond of a lactam bridge.	D	[Ref.28921993] The toxicity of sDRIM toward HEK293 and HeLa cells is much less than nonaarginine (R9) by use of flow cytometry and the peptide doesn't show any cytotoxicity at 2 μM.	28921993	J Med Chem. 2017 Oct 12;60(19):8071-8082. doi: 10.1021/acs.jmedchem.7b00813. Epub 2017 Sep 26.	Marco J Klein, Samuel Schmidt, Parvesh Wadhwani, Jochen Bürck, Johannes Reichert, Sergii Afonin, Marina Berditsch, Tim Schober, Roland Brock, Manfred Kansy, Anne S Ulrich	Lactam-Stapled Cell-Penetrating Peptides: Cell Uptake and Membrane Binding Properties.	Stapled AMP					
DRAMP21619	PLIⓀLRLⒹRGQF	PLIKLRLDRGQF	PLILLRLLRGQF	12	sWWSP	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①α-helix in aqueous solutions [pure water (H₂O), phosphate buffer (PB, 10 mM), and phosphate buffer with high salt (NaF, 100 mM)]. ②43% average α-helix content in various membranes environments [38% in POPC; 58% in POPC/POPG(3:1); 47% in POPC:lysoPC(9:1);	On the other hand, sWWSP and sMAP-1 assumed an α-helical conformation in aqueous solution, in contrast to their unstructured linear countparts.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.28921993] Gram-positive bacteria: Staphylococcus aureus (MIC > 256 μg/mL), Enterococcus faecalis (MIC > 256 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC > 256 μg/mL), Pseudomonas aeruginosa (MIC > 256 μg/mL)	[Ref.28921993] It has 4.0%, 4.8%, 4.6%, 4.2% and 7.0% hemolysis against human red blood cells at 15, 20, 25, 30 and 40 μg/ml.	Cyclic (Stapled)	Free	Amidation	Ⓚ (4) and Ⓓ (8) are cross-linked by lactam stapling through the polar amide bond of a lactam bridge.	L	[Ref.28921993] The toxicity of sWWSP toward HEK293 and HeLa cells is much less than nonaarginine (R9) by use of flow cytometry and the peptide doesn't show any cytotoxicity at 2 μM.	28921993	J Med Chem. 2017 Oct 12;60(19):8071-8082. doi: 10.1021/acs.jmedchem.7b00813. Epub 2017 Sep 26.	Marco J Klein, Samuel Schmidt, Parvesh Wadhwani, Jochen Bürck, Johannes Reichert, Sergii Afonin, Marina Berditsch, Tim Schober, Roland Brock, Manfred Kansy, Anne S Ulrich	Lactam-Stapled Cell-Penetrating Peptides: Cell Uptake and Membrane Binding Properties.	Stapled AMP					
DRAMP21621	AAⓀLLPⒹLLAAP	AAKLLPDLLAAP	AAVLLPVLLAAP	12	sKFGF	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①Disordered (or unstructured) conformation in aqueous solutions [pure water (H₂O), phosphate buffer (PB, 10 mM), and phosphate buffer with high salt (NaF, 100 mM)]. ②Around 22% average α-helix content in various membranes environments [13% in POPC; 32% in	In the cases of sDRIM and sKFGF, stapling did not induce any conformational change, as these analogues remained just as unstructured.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.28921993] Gram-positive bacteria: Staphylococcus aureus (MIC > 256 μg/mL), Enterococcus faecalis (MIC > 256 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC > 256 μg/mL), Pseudomonas aeruginosa (MIC > 256 μg/mL)	[Ref.28921993] It has 0.4%, 0%, 0.1%, 0.2%, 0.1%, 0.7%, 1.8% and 5.6% hemolysis against human red blood cells at 5, 7.5, 10, 15, 20, 25, 30 and 40 μg/ml.	Cyclic (Stapled)	Free	Amidation	Ⓚ (3) and Ⓓ (7) are cross-linked by lactam stapling through the polar amide bond of a lactam bridge.	L	[Ref.28921993] The toxicity of sKFGF toward HEK293 and HeLa cells is much less than nonaarginine (R9) by use of flow cytometry and the peptide doesn't show any cytotoxicity at 2 μM.	28921993	J Med Chem. 2017 Oct 12;60(19):8071-8082. doi: 10.1021/acs.jmedchem.7b00813. Epub 2017 Sep 26.	Marco J Klein, Samuel Schmidt, Parvesh Wadhwani, Jochen Bürck, Johannes Reichert, Sergii Afonin, Marina Berditsch, Tim Schober, Roland Brock, Manfred Kansy, Anne S Ulrich	Lactam-Stapled Cell-Penetrating Peptides: Cell Uptake and Membrane Binding Properties.	Stapled AMP					
DRAMP21623	KLALKALKⓀLKAⒹLKLA	KLALKALKKLKADLKLA	KLALKALKALKAALKLA	17	sMAP-1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		①α-helix in aqueous solutions [pure water (H₂O), phosphate buffer (PB, 10 mM), and phosphate buffer with high salt (NaF, 100 mM)]. ②46% average α-helix content in various membranes environments [37% in POPC; 59% in POPC/POPG(3:1); 45% in POPC:lysoPC(9:1);	On the other hand, sWWSP and sMAP-1 assumed an α-helical conformation in aqueous solution, in contrast to their unstructured linear countparts.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.28921993] Gram-positive bacteria: Staphylococcus aureus (MIC = 64 μg/mL), Enterococcus faecalis (MIC > 256 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 256 μg/mL), Pseudomonas aeruginosa (MIC > 256 μg/mL)	[Ref.28921993] It has 2.5%, 2.9%, 2.4%, 4.1%, 2.9%, 6.3%, 4.9% and 8.2% hemolysis against human red blood cells at 5, 7.5, 10, 15, 20, 25, 30 and 40 μg/ml.	Cyclic (Stapled)	Free	Amidation	Ⓚ (9) and Ⓓ (13) are cross-linked by lactam stapling through the polar amide bond of a lactam bridge.	L	[Ref.28921993] The toxicity of sMAP-1 toward HEK293 and HeLa cells is much less than nonaarginine (R9) by use of flow cytometry and the peptide doesn't show any cytotoxicity at 2 μM.	28921993	J Med Chem. 2017 Oct 12;60(19):8071-8082. doi: 10.1021/acs.jmedchem.7b00813. Epub 2017 Sep 26.	Marco J Klein, Samuel Schmidt, Parvesh Wadhwani, Jochen Bürck, Johannes Reichert, Sergii Afonin, Marina Berditsch, Tim Schober, Roland Brock, Manfred Kansy, Anne S Ulrich	Lactam-Stapled Cell-Penetrating Peptides: Cell Uptake and Membrane Binding Properties.	Stapled AMP					
DRAMP21624	ⓍLAAⒿRHⓍ	XLAAJRHX	ELAAIRHR	8	V30-SP-8	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+		26.1% α-helix content in 50 μM aqueous solution [a mixture of water and acetonitrile (9:1, v/v)].	①The helical propensities of two stapled peptides, V30-SP-8 and V30-SP-9, and their linear counterparts were analyzed via circular dichroism (CD) spectrometry. ②Surprisingly, in contrast to our expectation that the stitched peptide, V30-SP-8, would be more helical than the monostapled peptide, V30-SP-9, the monostapling strategy was more effective in inducing helicity (helicity: 26.1% for V30-SP-8 and 33.8% for V30-SP-9)	Not found	Function: Antibacterial activity against Gram-positive bacteria. The peptide shows a similar potency to vancomycin (MIC50 = 20 μM against M.smegmatis)	[Ref.32840352] Gram-positive bacteria: Mycobacterium smegmatis (The antibacterial effects of V30-SP-8 are 36.9%, 42.7%, 50.9% and 55.4% at 6.25, 12.5, 25 and 50 μM, and MIC50 is between 12.5 μM and 25.0 μM)	[Ref.32840352] No hemolytic activity information found.	Cyclic (Stapled)	Free	Free	①The Ⓧ (position: 1 and 8) in sequence indicates S5 stapling amino acid. Note: S5 is (S)-pentenyl alanine. ②The Ⓙ (position: 5) in sequence is B5 stapling amino acid. Note: B5 is α-dipentenyl alanine. ③Ⓧ (1) and Ⓙ (5), Ⓙ (5) and Ⓧ (8) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple respectively.	L	No cytotoxicity information found in the reference	32840352	ACS Chem Biol. 2020 Sep 18;15(9):2493-2498. doi: 10.1021/acschembio.0c00492. Epub 2020 Sep 9.	Sung-Min Kang, Heejo Moon, Sang-Woo Han, Do-Hee Kim, Byeong Moon Kim, Bong-Jin Lee	Structure-Based De Novo Design of Mycobacterium Tuberculosis VapC-Activating Stapled Peptides	Stapled AMP					
DRAMP21625	ⓏLAAIRHⓍ	ZLAAIRHX	ELAAIRHR	8	V30-SP-9	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+		33.8% α-helix content in 50 μM aqueous solution [a mixture of water and acetonitrile (9:1, v/v)].	①The helical propensities of two stapled peptides, V30-SP-8 and V30-SP-9, and their linear counterparts were analyzed via circular dichroism (CD) spectrometry. ②Surprisingly, in contrast to our expectation that the stitched peptide, V30-SP-8, would be more helical than the monostapled peptide, V30-SP-9, the monostapling strategy was more effective in inducing helicity (helicity: 26.1% for V30-SP-8 and 33.8% for V30-SP-9)	Not found	Function: Antibacterial activity against Gram-positive bacteria. 	[Ref.32840352] Gram-positive bacteria: Mycobacterium smegmatis (The antibacterial effects of V30-SP-8 are 23.3%, 23.3%, 34.2% and 38.7% at 6.25, 12.5, 25 and 50 μM, and MIC50 is above 25 μM)	[Ref.32840352] No hemolytic activity information found.	Cyclic (Stapled)	Free	Free	①The Ⓩ (position: 1) in sequence is R8 stapling amino acid. Note: R8 is (R)-octenyl alanine. ②The Ⓧ (position: 8) in sequence is (S)-pentenyl alanine. ③Ⓩ (1) and Ⓧ (8) are cross-linked by hydrocarbon stapling through a undec-4-enyl staple.	L	No cytotoxicity information found in the reference	32840352	ACS Chem Biol. 2020 Sep 18;15(9):2493-2498. doi: 10.1021/acschembio.0c00492. Epub 2020 Sep 9.	Sung-Min Kang, Heejo Moon, Sang-Woo Han, Do-Hee Kim, Byeong Moon Kim, Bong-Jin Lee	Structure-Based De Novo Design of Mycobacterium Tuberculosis VapC-Activating Stapled Peptides	Stapled AMP					
DRAMP21628	TLKQFⓍKGVⓍKWLVK	TLKQFXKGVXKWLVK	TLKQFAKGVGKWLVK	15	E2EM15W-S1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+		47% α-helical content in a 25 mM potassium phosphate buffer solution at 20 ℃.	①On the other hand, all three stapled analogs of E2EM15W showed substantial increases in helical contents, which again demonstrated the highly effective helix-stabilization through the all-hydrocarbon stapling technology. ②E2EM15W-S1, the most potent analog in the antimicrobial assay, showed the highest degree of helicity (47%) in the aqueous solution, supporting a close correlation between the helicity and the antimicrobial activity of the peptides in this series.	Not found	Function: Antibcaterial activity against Gram-positive bacteria. Antibacterial activity against Gram-negative bacteria is not noteable under 200 μg/mL.	[Ref.24211019] Gram-positive bacteria: Bacillus subtilis (MIC = 3.13 μg/mL), Staphylococcus aureus (MIC = 3.13 μg/mL), Staphylococcus epidermis (MIC > 200 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC > 200 μg/mL), Shigella dysentariae (MIC > 200 μg/mL), Salmonella typhimurium (MIC > 200 μg/mL), Klebsiella pneumonia (MIC > 200 μg/mL), Proteus mirabilis (MIC > 200 μg/mL), Pseudomonas aeuginose (MIC > 200 μg/mL).	[Ref.24211019] No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 6 and 10) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling through an oct-4-enyl staple.	L	No cytotoxicity information found in the reference	24211019	Bioorg Med Chem Lett. 2013 Dec 15;23(24):6717-20. doi: 10.1016/j.bmcl.2013.10.031. Epub 2013 Oct 26.	Thanh Kim Pham, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Truncated and constrained helical analogs of antimicrobial esculentin-2EM	Stapled AMP					
DRAMP21629	TLKQFⓍKGWⓍKDLVK	TLKQFXKGWXKDLVK	TLKQFAKGVGKWLVK	15	E2EM15W-S2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		27% α-helical content in a 25 mM potassium phosphate buffer solution at 20 ℃.	①On the other hand, all three stapled analogs of E2EM15W showed substantial increases in helical contents, which again demonstrated the highly effective helix-stabilization through the all-hydrocarbon stapling technology. ②Although they bear the oce-4-enyl staple at the same positions as in E2EM15W-S1, the other stapled derivatives E2EM15W-S2 and E2EM15W-S3 exhibited markedly smaller helical contents: 27% and 37%, repectively.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.24211019] Gram-positive bacteria: Bacillus subtilis (MIC = 6.25 μg/mL), Staphylococcus aureus (MIC = 6.25 μg/mL), Staphylococcus epidermis (MIC > 200 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 100 μg/mL), Shigella dysentariae (MIC = 50 μg/mL), Salmonella typhimurium (MIC > 200 μg/mL), Klebsiella pneumonia (MIC = 50 μg/mL), Proteus mirabilis (MIC > 200 μg/mL), Pseudomonas aeuginose (MIC = 200 μg/mL).	[Ref.24211019] No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 6 and 10) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling through an oct-4-enyl staple.	L	No cytotoxicity information found in the reference	24211019	Bioorg Med Chem Lett. 2013 Dec 15;23(24):6717-20. doi: 10.1016/j.bmcl.2013.10.031. Epub 2013 Oct 26.	Thanh Kim Pham, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Truncated and constrained helical analogs of antimicrobial esculentin-2EM	Stapled AMP					
DRAMP21630	TLKQWⓍKGVⓍKDLVK	TLKQWXKGVXKDLVK	TLKQFAKGVGKWLVK	15	E2EM15W-S3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		37% α-helical content in a 25 mM potassium phosphate buffer solution at 20 ℃.	①On the other hand, all three stapled analogs of E2EM15W showed substantial increases in helical contents, which again demonstrated the highly effective helix-stabilization through the all-hydrocarbon stapling technology. ②Although they bear the oce-4-enyl staple at the same positions as in E2EM15W-S1, the other stapled derivatives E2EM15W-S2 and E2EM15W-S3 exhibited markedly smaller helical contents: 27% and 37%, repectively.	Not found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.24211019] Gram-positive bacteria: Bacillus subtilis (MIC = 6.25 μg/mL), Staphylococcus aureus (MIC = 6.25 μg/mL), Staphylococcus epidermis (MIC = 100 μg/mL);##Gram-negative bacteria: Escherichia coli (MIC = 100 μg/mL), Shigella dysentariae (MIC = 50 μg/mL), Salmonella typhimurium (MIC > 200 μg/mL), Klebsiella pneumonia (MIC = 50 μg/mL), Proteus mirabilis (MIC > 200 μg/mL), Pseudomonas aeuginose (MIC > 200 μg/mL).	[Ref.24211019] No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 6 and 10) in sequence indicates (S)-α-methyl, α-pentenylglycine. ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling through an oct-4-enyl staple.	L	No cytotoxicity information found in the reference	24211019	Bioorg Med Chem Lett. 2013 Dec 15;23(24):6717-20. doi: 10.1016/j.bmcl.2013.10.031. Epub 2013 Oct 26.	Thanh Kim Pham, Do-Hee Kim, Bong-Jin Lee, Young-Woo Kim	Truncated and constrained helical analogs of antimicrobial esculentin-2EM	Stapled AMP					
DRAMP21651	TLDPPYFLDPVSPNPMCHRP			20	SLAY-screened peptide P1			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTTGACCCTCCCTATTTTCTCGATCCTGTCAGCCCCAATCCCATGTGCCACCGTCCCTAA	TLDPPYFLDPVSPNPMCHRP*	-11.199	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21652	LQSPDLQHFQYLLLLSGSRGL			21	SLAY-screened peptide P2			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCAGAGCCCTGACTTACAGCATTTTCAATACTTATTACTGTTATCAGGGTCCCGGGGTCTA	LQSPDLQHFQYLLLLSGSRGL	-11.079	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21653	QRRH			4	SLAY-screened peptide P3			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCGCCGGCATTAGCTGCACACGAGTTCGCTGTCCCCCAGTCTGGGCAGTTTTTGGGCTTAA	QRRH*LHTSSLSPSLGSFWA*	-10.843	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21654	AGAAFNSCAR			10	SLAY-screened peptide P4			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGGGGCGGCTTTCAATTCCTGTGCCAGGTAGGATGACAACTTCTACATCTATTATGCGTAA	AGAAFNSCAR*DDNFYIYYA*	-10.83	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21655	SSHLPHHGCNRRFVDGPAPPQ			21	SLAY-screened peptide P5			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTCGCACCTTCCCCATCACGGTTGTAACCGCCGTTTTGTGGACGGCCCCGCCCCCCCCCAA	SSHLPHHGCNRRFVDGPAPPQ	-10.803	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21656	NATMLCLSDNFCNENFTHQA			20	SLAY-screened peptide P6			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGCTACTATGCTCTGCCTTTCCGATAATTTTTGCAACGAGAATTTTACGCATCAGGCCTAA	NATMLCLSDNFCNENFTHQA*	-10.8	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21657	GDS			3	SLAY-screened peptide P7			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGATAGCTAGTGTTTCAAGTATACTTATCCCTGGAATAATGACTACCACGTTAGCGCGTAA	GDS*CFKYTYPWNNDYHVSA*	-10.604	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21658	IHPLSFR			7	SLAY-screened peptide P8			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCATCCCCTCTCCTTTCGTTAGTATCGCAACGTTCGGACCATTAATTCGTCCGCTGTGTAA	IHPLSFR*YRNVRTINSSAV*	-10.57	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21659	WYVFSLAVAPVNNTNRDGSP			20	SLAY-screened peptide P9			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTACGTCTTCAGTCTCGCCGTCGCGCCTGTTAACAATACGAATCGCGATGGGTCCCCTTAA	WYVFSLAVAPVNNTNRDGSP*	-10.462	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21660	PSNAVMPINARYKSGYSPAS			20	SLAY-screened peptide P10			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGCAATGCTGTTATGCCCATTAATGCCCGCTACAAGAGCGGTTATTCGCCTGCCTCTTAA	PSNAVMPINARYKSGYSPAS*	-10.364	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21661	NNLYHTHGNCYKDTNINFEN			20	SLAY-screened peptide P11			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATAATCTCTACCACACTCATGGGAATTGCTACAAGGACACCAATATTAATTTTGAGAACTAA	NNLYHTHGNCYKDTNINFEN*	-10.274	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21662	HLLPVISI			8	SLAY-screened peptide P12			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCTGCTCCCCGTCATCTCTATCTAGCATCAGGTCGTCTCCGTGGGTCATGACGCGCTGTAA	HLLPVISI*HQVVSVGHDAL*	-10.157	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21663	LTASRAPGSLPTVWLPIVLLN			21	SLAY-screened peptide P13			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACTGCGTCTCGAGCACCGGGGTCATTACCAACTGTTTGGCTCCCTATAGTACTACTTAAC	LTASRAPGSLPTVWLPIVLLN	-10.141	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21664	IG			2	SLAY-screened peptide P14			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGGTTAGGGTGACCTTTACATCACTGAGACTTAGGATTATAATAGTAGTCTTTTTGATTAA	IG*GDLYITET*DYNSSLFD*	-10.056	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21665	APMGYSSVASSMSTSSYFID			20	SLAY-screened peptide P15			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCCATGGGTTATTCTTCTGTCGCGTCTAGCATGTCTACTTCTTCTTACTTTATTGACTAA	APMGYSSVASSMSTSSYFID*	-10.041	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21666	RPRLSGIMTYYVSTWISYIC			20	SLAY-screened peptide P16			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCCCGTCTTAGCGGGATCATGACCTATTACGTTTCCACTTGGATCAGCTACATTTGTTAA	RPRLSGIMTYYVSTWISYIC*	-10.03	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21667	LSGERRHTVGVQTMHSDHME			20	SLAY-screened peptide P17			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTCTGGTGAGAGGAGGCACACTGTCGGTGTCCAGACCATGCATTCTGATCATATGGAGTAA	LSGERRHTVGVQTMHSDHME*	-9.883	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21668	QSKPDATQPYVHYCKRRLLR			20	SLAY-screened peptide P18			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTCCAAGCCTGACGCTACTCAGCCTTATGTCCATTACTGCAAGCGTCGTCTCCTGCGTTAA	QSKPDATQPYVHYCKRRLLR*	-9.861	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21669	PCATALIPSPRQDSRTL			17	SLAY-screened peptide P19			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGCGCTACGGCCCTCATTCCTTCGCCTCGGCAGGATTCCCGGACCCTGTAGATTAAGTAA	PCATALIPSPRQDSRTL*IK*	-9.719	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21670	GCLNFSVPVDRPVSPAKTAW			20	SLAY-screened peptide P20			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTGTCTGAACTTTTCTGTCCCCGTGGACCGGCCTGTGTCGCCTGCCAAGACGGCCTGGTAA	GCLNFSVPVDRPVSPAKTAW*	-9.619	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21671	RVVILMLS			8	SLAY-screened peptide P21			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGTCGTCATCTTGATGTTGTCCTAGAATGCTTGTCATCAGTTGCATCTGACGATTTCTTAA	RVVILMLS*NACHQLHLTIS*	-9.558	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21672	FRCPPFKFSCLALAFTDYNN			20	SLAY-screened peptide P22			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCGGTGCCCGCCGTTTAAGTTCTCCTGTCTTGCCCTTGCTTTTACGGATTATAACAATTAA	FRCPPFKFSCLALAFTDYNN*	-9.504	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21673	PVRCVTPTSPCAPNPHYHDQ			20	SLAY-screened peptide P23			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGTGCGTTGTGTCACGCCTACTTCTCCGTGTGCTCCTAATCCTCACTACCATGATCAGTAA	PVRCVTPTSPCAPNPHYHDQ*	-9.45	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21674	YAFFINNDCFYYCSLGPCASN			21	SLAY-screened peptide P24			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGCCTTTTTTATCAATAATGATTGTTTTTATTATTGTTCTTTAGGCCCATGTGCATCTAAC	YAFFINNDCFYYCSLGPCASN	-9.396	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21675	PLAPIVQTYS			10	SLAY-screened peptide P25			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTTGCCCCGATTGTTCAGACGTACTCTTAGAGCACTAATCTGTATCGCGGTTATTGCTAA	PLAPIVQTYS*STNLYRGYC*	-9.352	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21676	SWHWLSSNHIATVSVETYSH			20	SLAY-screened peptide P26			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTGGCACTGGCTTTCTTCTAATCATATCGCGACCGTGAGCGTTGAGACCTACTCCCACTAA	SWHWLSSNHIATVSVETYSH*	-9.321	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21677	TLSVFFCIHPPPCSVSTSPY			20	SLAY-screened peptide P27			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTCTCCGTCTTTTTCTGCATCCACCCCCCCCCCTGTAGTGTCTCTACGTCTCCGTATTAA	TLSVFFCIHPPPCSVSTSPY*	-9.161	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21678	YLLARLALQTFFSHGFYTFP			20	SLAY-screened peptide P28			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCTGCTCGCTCGGCTCGCCCTGCAGACGTTTTTCAGTCACGGTTTCTATACTTTTCCTTAA	YLLARLALQTFFSHGFYTFP*	-9.158	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21679	LTLLICPDDTFYKAK			15	SLAY-screened peptide P29			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACCTTGCTTATTTGCCCCGATGATACTTTTTATAAGGCGAAGTAGGCCTCTCCTTTTTAA	LTLLICPDDTFYKAK*ASPF*	-9.154	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21680	NLFPSHSATFRH			12	SLAY-screened peptide P30			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTTGTTCCCGAGCCATTCTGCCACCTTTCGGCATTAGGATTATATTCTGCGTTTTCGTTAA	NLFPSHSATFRH*DYILRFR*	-9.146	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21681	TSDYILVQFYFS			12	SLAY-screened peptide P31			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCTGATTACATTTTGGTTCAGTTTTATTTTTCTTAGACGCTCACCCACCTCAACTGTTAA	TSDYILVQFYFS*TLTHLNC*	-9.104	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21682	PCISQSNDVCPSRESLPLCI			20	SLAY-screened peptide P32			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGTATCAGCCAGTCGAACGACGTCTGCCCGTCGCGTGAGTCTTTGCCTCTGTGTATTTAA	PCISQSNDVCPSRESLPLCI*	-9.092	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21683	GHAHPCTNFIYDINLNPPPPP			21	SLAY-screened peptide P33			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCACGCCCACCCTTGCACGAACTTTATCTACGACATTAACCTGAACCCCCCCCCCCCCCCC	GHAHPCTNFIYDINLNPPPPP	-8.993	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21684	YASHCPCRTICYHVSP			16	SLAY-screened peptide P34			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGCCTCCCACTGCCCCTGTCGCACCATTTGTTATCACGTTTCGCCGTAGTACTCCAGGTAA	YASHCPCRTICYHVSP*YSR*	-8.968	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21685	SKCSITTRRQYAHPRSAV			18	SLAY-screened peptide P35			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCAAGTGCAGTATCACTACCAGGAGGCAGTACGCGCACCCTCGTAGTGCGGTTTAGAATTAA	SKCSITTRRQYAHPRSAV*N*	-8.946	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21686	TREATPCARIRSDSFGTT			18	SLAY-screened peptide P36			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCGTGAGGCGACTCCCTGCGCGCGTATTCGCTCCGACTCTTTTGGGACGACCTAGCCCTAA	TREATPCARIRSDSFGTT*P*	-8.937	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21687	TPMDRSLCHNHTL			13	SLAY-screened peptide P37			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCTATGGATCGTAGCCTCTGTCATAACCATACGCTTTAGATGGCGCAGGATAGTCATTAA	TPMDRSLCHNHTL*MAQDSH*	-8.916	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21688	SVRSSAPLMRVIGNCPSNHH			20	SLAY-screened peptide P38			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGTGCGTTCTAGCGCGCCTCTTATGCGTGTGATCGGCAATTGCCCGAGTAATCACCACTAA	SVRSSAPLMRVIGNCPSNHH*	-8.863	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21689	LLYEVDPAT			9	SLAY-screened peptide P39			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTTTACGAGGTCGACCCTGCCACTTAGTTCCATTCCCCCCCCCCCGCCCCCCCCCCCCCT	LLYEVDPAT*FHSPPPAPPPP	-8.836	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21690	SGSSPRNTQTP			11	SLAY-screened peptide P40			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGGGTCGTCGCCGCGGAATACGCAGACGCCCTAGGATTATTGCACTATGGTTCACACGTAA	SGSSPRNTQTP*DYCTMVHT*	-8.805	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21691	ANPAYKFKTCILCL			14	SLAY-screened peptide P41			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAATCCTGCTTATAAGTTTAAAACATGTATACTATGTCTGTGAGGGGGTTCGACAACTAAC	ANPAYKFKTCILCL*GGSTTN	-8.804	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21692	HTSNEDKTVYPVHSECIFDY			20	SLAY-screened peptide P42			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACCAGTAATGAGGATAAGACCGTGTATCCGGTTCACTCTGAGTGTATTTTTGACTATTAA	HTSNEDKTVYPVHSECIFDY*	-8.804	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21693	LYAEVGRLLIDLGAT			15	SLAY-screened peptide P43			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTACGCGGAGGTGGGGCGTCTCCTGATCGACCTTGGGGCCACCTAACTGAGTAAGTCGACC	LYAEVGRLLIDLGAT*LSKST	-8.765	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21694	RLDLASPFDIGIEGLSPANL			20	SLAY-screened peptide P44			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTCGATCTCGCTTCGCCTTTTGATATTGGTATTGAGGGTCTCTCCCCGGCTAACCTTTAA	RLDLASPFDIGIEGLSPANL*	-8.762	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21695	FYVPLRSSQPQPPISCRHTP			20	SLAY-screened peptide P45			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTACGTTCCCCTCCGGAGCTCCCAGCCTCAGCCCCCCATTTCCTGCCGCCACACCCCCTAA	FYVPLRSSQPQPPISCRHTP*	-8.749	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21696	LLVSSPSMRPIAVTPSGPAPN			21	SLAY-screened peptide P46			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTCGTCAGTTCTCCCTCCATGCGCCCGATAGCAGTAACCCCCTCGGGTCCTGCCCCTAAC	LLVSSPSMRPIAVTPSGPAPN	-8.745	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21697	THHMTGGVSWRGAPSLPYVN			20	SLAY-screened peptide P47			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCACCACATGACCGGCGGTGTCAGTTGGAGGGGGGCCCCCAGCCTCCCTTATGTCAATTAA	THHMTGGVSWRGAPSLPYVN*	-8.737	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21698	THHMTGGVSWRGAPSLPYVN			20	SLAY-screened peptide P48			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCATCACATGACCGGCGGTGTCAGTTGGAGGGGGGCCCCCAGCCTCCCTTATGTCAATTAA	THHMTGGVSWRGAPSLPYVN*	-8.737	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21699	AYRRLPLHARSPTVRVNLET			20	SLAY-screened peptide P49			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTACCGCCGTTTGCCCCTTCATGCTCGTTCTCCCACTGTTCGCGTTAATCTGGAGACGTAA	AYRRLPLHARSPTVRVNLET*	-8.731	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21700	SPDFLRCSHTSRFVAYLLLS			20	SLAY-screened peptide P50			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCCGGATTTCCTGCGGTGCAGTCATACGTCTCGCTTTGTCGCCTATTTGTTGCTCTCGTAA	SPDFLRCSHTSRFVAYLLLS*	-8.723	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21701	EYPCILTQTAVNNSNSDTVY			20	SLAY-screened peptide P51			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGTACCCCTGCATTCTGACCCAGACTGCTGTTAACAACTCGAATTCCGATACGGTGTATTAA	EYPCILTQTAVNNSNSDTVY*	-8.716	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21702	RPSIAPRFSPIGSDNMLISF			20	SLAY-screened peptide P52			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCGTCTATTGCCCCTCGTTTTAGTCCTATCGGTAGTGACAATATGCTCATTTCTTTTTAA	RPSIAPRFSPIGSDNMLISF*	-8.714	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21703	CTGYHKLNARDTVNSDISSS			20	SLAY-screened peptide P53			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTACCGGCTACCATAAGCTTAATGCCAGGGACACTGTTAATTCCGATATTTCGTCCAGTTAA	CTGYHKLNARDTVNSDISSS*	-8.684	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21704	IRVSNQSGLYGCPITLDWRL			20	SLAY-screened peptide P54			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCGTGTTTCTAATCAGTCCGGCCTTTACGGTTGTCCTATCACTCTCGATTGGCGCCTGTAA	IRVSNQSGLYGCPITLDWRL*	-8.667	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21705	DYRCGTRRFTIWAHLLGI			18	SLAY-screened peptide P55			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTACCGTTGTGGTACTCGTCGGTTTACGATTTGGGCTCATCTCTTGGGCATTTAGGTTTAA	DYRCGTRRFTIWAHLLGI*V*	-8.653	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21706	TGADGAHSCLITHYTENYGN			20	SLAY-screened peptide P56			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGGGGCGGACGGCGCTCACAGCTGCTTGATTACGCACTATACTGAGAATTATGGCAATTAA	TGADGAHSCLITHYTENYGN*	-8.641	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21707	IVLGAIHHYSSPSALSRVLQ			20	SLAY-screened peptide P57			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGTCCTTGGCGCGATTCATCATTATTCTTCTCCTTCTGCTCTGTCTCGCGTCCTCCAGTAA	IVLGAIHHYSSPSALSRVLQ*	-8.636	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21708	ANLLIWLGLYLSHQNRRVDD			20	SLAY-screened peptide P58			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTAACCTCCTGATTTGGCTCGGGTTGTATCTTTCCCACCAGAATAGGCGGGTCGACGATTAA	ANLLIWLGLYLSHQNRRVDD*	-8.62	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21709	LDPSYIFLDSSPMLRAESIN			20	SLAY-screened peptide P59			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGGATCCTTCTTATATCTTTCTCGACTCGTCGCCGATGCTTCGGGCGGAGAGCATTAACTAA	LDPSYIFLDSSPMLRAESIN*	-8.59	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21710	GNHLACLGVRLIRGFNLHHL			20	SLAY-screened peptide P60			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCAATCATCTGGCTTGCTTGGGTGTTCGCCTTATTCGTGGCTTTAACCTGCATCATTTGTAA	GNHLACLGVRLIRGFNLHHL*	-8.552	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21711	HGVHHLNDHLSFLTLNLSLH			20	SLAY-screened peptide P61			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGGCGTTCATCACCTTAACGATCACTTGTCGTTTCTGACCCTTAATCTTTCCCTTCATTAA	HGVHHLNDHLSFLTLNLSLH*	-8.52	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21712	IRSCLRTVRLLVTTHYYHRE			20	SLAY-screened peptide P62			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCGTTCTTGTCTCCGTACGGTTCGTCTCCTTGTCACTACGCATTATTATCATCGCGAGTAA	IRSCLRTVRLLVTTHYYHRE*	-8.497	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21713	YDLDRGCAYNLLVYAERYYQ			20	SLAY-screened peptide P63			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGACTTGGATCGGGGTTGTGCTTATAATCTCCTTGTCTATGCGGAGCGTTACTATCAGTAA	YDLDRGCAYNLLVYAERYYQ*	-8.477	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21714	RNLHLTASPVRVPRHRPINS			20	SLAY-screened peptide P64			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAACCTTCATCTCACGGCGTCCCCGGTGCGTGTCCCGAGGCATCGTCCGATCAATAGTTAA	RNLHLTASPVRVPRHRPINS*	-8.421	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21715	RSSFHRIIYFIENHHIKNAI			20	SLAY-screened peptide P65			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTCTAGCTTTCATCGCATTATTTACTTCATTGAGAATCATCATATCAAGAACGCGATCTAA	RSSFHRIIYFIENHHIKNAI*	-8.419	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21716	QLTMNNPRMPSSA			13	SLAY-screened peptide P66			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCTTACTATGAACAACCCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACGCCATTTAA	QLTMNNPRMPSSA*KKKNAI*	-8.411	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21717	RCPHISASYVVLPGVIHSTT			20	SLAY-screened peptide P67			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTGCCCCCACATTAGTGCCAGCTATGTTGTTCTTCCCGGTGTTATCCATTCGACGACCTAA	RCPHISASYVVLPGVIHSTT*	-8.4	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21718	RRVRHRILSDIRVAHYRRWP			20	SLAY-screened peptide P68			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCGCGTCCGCCATCGTATCCTTAGTGACATCCGCGTGGCGCATTATAGGAGGTGGCCGTAA	RRVRHRILSDIRVAHYRRWP*	-8.381	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21719	TRTSSQTVAGNPRYNNSERS			20	SLAY-screened peptide P69			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCGCACTTCGTCTCAGACCGTTGCTGGTAATCCCAGGTACAATAATTCTGAGCGGTCCTAA	TRTSSQTVAGNPRYNNSERS*	-8.38	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21720	ALAYFHRVCA			10	SLAY-screened peptide P70			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCTGGCGTATTTCCATCGGGTTTGCGCTTAGGATCAGAGTGTCGTGTGCGTCACTTGGTAA	ALAYFHRVCA*DQSVVCVTW*	-8.365	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21721	SHNPHIRGPIQRSRKRPRRT			20	SLAY-screened peptide P71			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCATAACCCTCATATTCGGGGCCCCATCCAGAGGTCTCGTAAGCGTCCTAGGAGGACCTAA	SHNPHIRGPIQRSRKRPRRT*	-8.345	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21722	RSPCAPYAPPPLTFFRTVSA			20	SLAY-screened peptide P72			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAGTCCTTGCGCGCCGTACGCCCCCCCCCCTCTTACTTTCTTCCGTACCGTCAGTGCTTAA	RSPCAPYAPPPLTFFRTVSA*	-8.329	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21723	CTPAPPGIPCCSAYTFYYNR			20	SLAY-screened peptide P73			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTACCCCGGCGCCCCCTGGGATTCCTTGTTGTTCGGCTTACACTTTTTATTATAATCGCTAA	CTPAPPGIPCCSAYTFYYNR*	-8.325	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21724	STVQYHWNNSPFDSHARRTI			20	SLAY-screened peptide P74			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTACCGTCCAGTATCATTGGAATAATAGTCCTTTTGACAGTCATGCTCGCCGGACGATCTAA	STVQYHWNNSPFDSHARRTI*	-8.161	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21725	LTFHCHHNNDCNFNYLSSTL			20	SLAY-screened peptide P75			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACCTTTCATTGCCATCATAATAATGATTGTAATTTCAATTATCTGAGCAGTACTCTGTAA	LTFHCHHNNDCNFNYLSSTL*	-8.127	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21726	PKHSYSNVLA			10	SLAY-screened peptide P76			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAAGCATAGTTACAGTAACGTTTTGGCTTAGTATGACAACCTGGGTTACACTAGTAATTAA	PKHSYSNVLA*YDNLGYTSN*	-8.121	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21727	SSNYRQSECYDTSSFTYVLI			20	SLAY-screened peptide P77			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTCTAATTATCGCCAGTCGGAGTGTTACGATACCTCTTCCTTTACGTACGTCCTTATTTAA	SSNYRQSECYDTSSFTYVLI*	-8.093	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21728	IGDVMATVATLINASSLYFP			20	SLAY-screened peptide P78			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGGCGACGTTATGGCTACTGTTGCTACTCTTATTAATGCTTCTAGCCTTTACTTTCCTTAA	IGDVMATVATLINASSLYFP*	-8.09	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21729	NVILRNSGLHASICSPPPPPP			21	SLAY-screened peptide P79			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGTCATCCTGCGCAACAGCGGGCTCCACGCTAGCATCTGTTCCCCCCCCCCCCCCCCCCCC	NVILRNSGLHASICSPPPPPP	-8.039	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21730	VASVFNCRNCLSYSNPNDTP			20	SLAY-screened peptide P80			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGCGTCTGTGTTCAATTGCCGTAATTGTCTTTCTTATTCGAATCCTAATGACACTCCTTAA	VASVFNCRNCLSYSNPNDTP*	-6.795	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21731	TASHSSSQYPKT			12	SLAY-screened peptide P81			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGCGAGTCATAGTTCGTCTCAGTATCCTAAGACGTAGGTCTAGACTCTGACTATTTCTTAA	TASHSSSQYPKT*V*TLTIS*	-6.774	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21732	LWNWDCFCFLRYHFGKRTTN			20	SLAY-screened peptide P82			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTGGAACTGGGATTGTTTCTGTTTCCTTCGTTATCACTTTGGGAAGCGTACCACTAATTAA	LWNWDCFCFLRYHFGKRTTN*	-6.704	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21733	PLLHIFNSTAMYIY			14	SLAY-screened peptide P83			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTCCTGCATATTTTTAATTCTACCGCTATGTATATTTATTAGATCAACGCGCACAATTAA	PLLHIFNSTAMYIY*INAHN*	-6.629	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21734	PYGASTANIDFLDVFIYNTT			20	SLAY-screened peptide P84			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTACGGGGCGAGCACTGCGAATATTGATTTTCTGGATGTGTTTATCTACAATACGACGTAA	PYGASTANIDFLDVFIYNTT*	-6.558	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21735	SS			2	SLAY-screened peptide P85			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTCTTAGACTTTTGTTTCTATCTGCTCTACGATGTACTCCGACTTCTGCACTTATGCCTAA	SS*TFVSICSTMYSDFCTYA*	-6.536	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21736	TK			2	SLAY-screened peptide P86			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAAGTAGCATAATAAGGCGGTCAATTATAAGCGTTCTGTGTCTATTGAGACTGATTTTTAA	TK*HNKAVNYKRSVSIETDF*	-6.471	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21737	STLCIQSRPSNTSCIHLAKN			20	SLAY-screened peptide P87			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGACGCTGTGTATTCAGTCTCGTCCTTCGAATACCTCCTGTATCCACCTTGCGAAGAACTAA	STLCIQSRPSNTSCIHLAKN*	-6.43	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21738	TIRLHVSIRIYLWRRRMVSA			20	SLAY-screened peptide P88			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTATCCGGTTGCATGTCAGCATCCGCATTTACCTTTGGAGGCGCCGCATGGTGTCTGCGTAA	TIRLHVSIRIYLWRRRMVSA*	-6.219	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21739	TQLYHTWH			8	SLAY-screened peptide P89			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCAGCTGTATCATACGTGGCACTAGACGAATAATGAGACTATTCCTAACTATAATGCGTAA	TQLYHTWH*TNNETIPNYNA*	-6.152	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21740	LPLKASQH			8	SLAY-screened peptide P90			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCGCTTAAGGCTAGCCAGCACTAGAACGTGTGTCGTACTCAGACTGGTAATAATGCTTAA	LPLKASQH*NVCRTQTGNNA*	-5.974	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21741	CALIIIFFYVRVCVRVSLTC			20	SLAY-screened peptide P91			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCTTTGATCATTATTTTTTTCTACGTTCGGGTTTGCGTGCGTGTGAGTCTGACGTGCTAA	CALIIIFFYVRVCVRVSLTC*	-5.958	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21742	YGRSHATPNSDVSSMSPITA			20	SLAY-screened peptide P92			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGGTAGGTCTCATGCTACTCCTAATAGTGACGTGTCTAGCATGAGTCCGATCACTGCCTAA	YGRSHATPNSDVSSMSPITA*	-5.944	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21743	RLAHFPNHAVCDPHIINKPL			20	SLAY-screened peptide P93			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCTCGCTCACTTTCCGAATCATGCTGTCTGCGATCCTCATATTATCAATAAGCCGCTTTAA	RLAHFPNHAVCDPHIINKPL*	-5.777	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21744	RLGHDSNPWHIFRYNNNIPI			20	SLAY-screened peptide P94			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTTGGGTCATGATAGCAACCCTTGGCATATTTTCCGTTATAATAACAATATCCCCATTTAA	RLGHDSNPWHIFRYNNNIPI*	-5.759	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21745	RYHTHYC			7	SLAY-screened peptide P95			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTACCACACGCACTACTGCTAGGCTACGAATAATTATTTTAATGACGATTATTTTGCCTAA	RYHTHYC*ATNNYFNDDYFA*	-5.733	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21746	LQLSPRYVSRSYDCPTPLTT			20	SLAY-screened peptide P96			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCAGCTTAGCCCTCGTTATGTTTCGCGCAGTTATGATTGCCCTACTCCTCTCACTACTTAA	LQLSPRYVSRSYDCPTPLTT*	-5.69	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21747	RRCPPSSFAGHDPHRPIY			18	SLAY-screened peptide P97			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCGGTGCCCTCCTTCTTCGTTTGCTGGTCATGACCCTCATAGGCCTATTTATTAGATCTAA	RRCPPSSFAGHDPHRPIY*I*	-5.652	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21748	SSWAGHTRCGRCHPRYCYVT			20	SLAY-screened peptide P98			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTCTTGGGCTGGCCATACTCGCTGTGGCCGTTGCCATCCTAGGTACTGTTATGTCACTTAA	SSWAGHTRCGRCHPRYCYVT*	-5.625	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21749	LYCNHHTTLRCPKITVQNTR			20	SLAY-screened peptide P99			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTACTGTAATCATCACACTACGCTGCGTTGTCCTAAGATTACGGTCCAGAATACCAGGTAA	LYCNHHTTLRCPKITVQNTR*	-5.55	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21750	GHCSQIRFTACPIHALCNGT			20	SLAY-screened peptide P100			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCATTGTTCTCAGATTCGTTTTACGGCTTGCCCTATCCATGCGCTGTGCAACGGTACTTAA	GHCSQIRFTACPIHALCNGT*	-5.494	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21751	LYMFNSTMSNVAYEFI			16	SLAY-screened peptide P101			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTATATGTTCAATAGTACTATGTCTAATGTCGCTTATGAGTTCATCTAGCCGAAGCCTTAA	LYMFNSTMSNVAYEFI*PKP*	-5.475	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21752	YVTASNLYFVNCFTMFVMAK			20	SLAY-screened peptide P102			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGTTACTGCGTCCAATCTGTATTTTGTCAATTGCTTTACTATGTTCGTTATGGCTAAGTAA	YVTASNLYFVNCFTMFVMAK*	-5.442	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21753	RRDCNIESHYLRTPRS			16	SLAY-screened peptide P103			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGTGATTGTAATATTGAGTCTCACTATCTGCGGACGCCTCGTTCGTAACTGAGTAAGTCG	RRDCNIESHYLRTPRS*LSKS	-5.421	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21754	GSLTSIDRCELDHVGYIHYK			20	SLAY-screened peptide P104			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCAGTCTTACTAGTATTGATCGGTGCGAGCTGGACCATGTTGGTTATATCCATTACAAGTAA	GSLTSIDRCELDHVGYIHYK*	-5.395	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21755	FVTQYSPFLGYFAPTRCSVP			20	SLAY-screened peptide P105			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGTCACTCAGTACAGCCCTTTTCTTGGCTATTTTGCTCCTACGCGTTGTTCCGTTCCGTAA	FVTQYSPFLGYFAPTRCSVP*	-5.357	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21756	IVFSGHDLQTDYLNNRIHLV			20	SLAY-screened peptide P106			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGTGTTTTCGGGGCACGATCTGCAGACTGACTATCTTAATAACAGGATCCACCTGGTGTAA	IVFSGHDLQTDYLNNRIHLV*	-5.343	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21757	PSFPSIYIRLSRIRHRHRRG			20	SLAY-screened peptide P107			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCCTTTCCGTCTATCTACATTCGTCTCTCCCGCATCCGTCACCGGCATCGTCGTGGCTAA	PSFPSIYIRLSRIRHRHRRG*	-5.325	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21758	ADSQHAPP			8	SLAY-screened peptide P108			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGACTCGCAGCACGCCCCTCCTTAGAATCACTATAAGTTTTATGATATTAGCGAGCCCTAA	ADSQHAPP*NHYKFYDISEP*	-5.287	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21759	YPYDRLSNVFDSLHYYCIQT			20	SLAY-screened peptide P109			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCCTTATGATCGCTTGTCTAACGTGTTCGATAGCCTTCATTATTATTGTATTCAGACCTAA	YPYDRLSNVFDSLHYYCIQT*	-5.205	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21760	PQLFTNHTPDSSYGIILAL			19	SLAY-screened peptide P110			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCAGCTGTTTACCAATCACACTCCTGATTCTAGCTATGGCATTATTCTTGCTTTGTAGTAA	PQLFTNHTPDSSYGIILAL**	-5.112	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21761	ERAPSYHTRSSSDSSNSGET			20	SLAY-screened peptide P111			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCGTGCTCCTAGTTATCATACTCGGAGCTCGAGTGACTCGAGCAATAGCGGTGAGACCTAA	ERAPSYHTRSSSDSSNSGET*	-5.096	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21762	TLVHNDSLSAQEPPPLSQ			18	SLAY-screened peptide P112			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTTGTGCATAATGATAGTTTGTCTGCTCAGGAGCCGCCGCCTCTGTCTCAGTAGGCTTAA	TLVHNDSLSAQEPPPLSQ*A*	-5.093	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21763	SHNCIHYP			8	SLAY-screened peptide P113			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCACAATTGTATTCACTATCCTTAGATGGATTTGTAGAACATGGCTCTGAAGAATGGGTAA	SHNCIHYP*MDL*NMALKNG*	-5.025	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21764	CPVQQSTYDKCSQPYRDTQH			20	SLAY-screened peptide P114			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCGGTTCAGCAGAGCACTTACGATAAGTGTTCTCAGCCTTACCGTGATACTCAGCATTAA	CPVQQSTYDKCSQPYRDTQH*	-4.993	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21765	RPYPPNFRRTPTQLPHLLVS			20	SLAY-screened peptide P115			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCCTATCCGCCGAACTTCAGGCGTACTCCCACCCAGCTTCCGCATCTTCTGGTCTCTTAA	RPYPPNFRRTPTQLPHLLVS*	-4.902	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21766	KRELT			5	SLAY-screened peptide P116			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCGCGAGTTGACTTAGATGCAGGCCCCTAAGCCTTTTATTTTTTTTGCGAATCACTGCTAA	KRELT*MQAPKPFIFFANHC*	-4.755	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21767	FDNTRMFCTIDIYNTDLHMH			20	SLAY-screened peptide P117			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGATAACACCCGTATGTTCTGTACCATTGATATCTATAACACTGATTTGCATATGCATTAA	FDNTRMFCTIDIYNTDLHMH*	-4.737	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21768	DDPIVFVSRTNVLPHY			16	SLAY-screened peptide P118			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGACCCTATTGTTTTCGTCTCTCGTACGAATGTGTTGCCGCACTATTAGCATGCGGCGTAA	DDPIVFVSRTNVLPHY*HAA*	-4.637	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21769	TPNVYHNGDGRVPLHCSLSL			20	SLAY-screened peptide P119			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCCGAACGTTTATCACAATGGCGACGGTCGTGTGCCTCTTCATTGCAGTCTGTCGCTCTAA	TPNVYHNGDGRVPLHCSLSL*	-4.628	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21770	RFRGHHNVNSWFVIFSHHHD			20	SLAY-screened peptide P120			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTTTCGGGGTCACCATAATGTTAATTCTTGGTTTGTCATTTTTTCTCATCACCATGATTAA	RFRGHHNVNSWFVIFSHHHD*	-4.61	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21771	PT			2	SLAY-screened peptide P121			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACCTAGCTGGTTTGGAGTGAGTATTTGCGTCTCTATCACGTTATTCTTTTTGCTCTTTAA	PT*LVWSEYLRLYHVILFAL*	-4.604	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21772	RTEVLPYRNTQSGIPNYEFS			20	SLAY-screened peptide P122			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGACTGAGGTTCTCCCTTACCGTAATACGCAGTCTGGTATTCCGAATTATGAGTTTAGTTAA	RTEVLPYRNTQSGIPNYEFS*	-4.593	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21773	ADMLLHRSNSNEHDHCAILL			20	SLAY-screened peptide P123			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGACATGCTGTTGCATCGGTCGAACAGTAACGAGCATGATCATTGTGCGATTTTGCTCTAA	ADMLLHRSNSNEHDHCAILL*	-4.587	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21774	VPLAVASEPGPTLNGPPRAT			20	SLAY-screened peptide P124			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCCCCTCGCCGTCGCTAGCGAGCCTGGCCCCACTTTGAACGGCCCTCCCCGGGCCACTTAA	VPLAVASEPGPTLNGPPRAT*	-4.526	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21775	TFAITDMFSETNSITRFN			18	SLAY-screened peptide P125			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTTTGCTATTACTGACATGTTCTCGGAGACCAATAGTATTACTCGTTTTAACTAGCTCTAA	TFAITDMFSETNSITRFN*L*	-4.493	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21776	LLPPGDLYQNRHIFPECNHN			20	SLAY-screened peptide P126			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCTGCCTCCTGGCGATCTCTATCAGAATCGCCATATCTTCCCGGAGTGCAACCATAATTAA	LLPPGDLYQNRHIFPECNHN*	-4.489	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21777	PHGHSFHVYISLLFY			15	SLAY-screened peptide P127			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCACGGGCATAGCTTTCACGTCTATATTTCTCTTCTTTTTTACTAGGGGCTGGTGAATTAA	PHGHSFHVYISLLFY*GLVN*	-4.481	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21778	CRTTSNHPLEIRRYCMYHGR			20	SLAY-screened peptide P128			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGTACTACTAGTAATCATCCTCTGGAGATTCGGAGGTATTGCATGTACCACGGGAGGTAA	CRTTSNHPLEIRRYCMYHGR*	-4.468	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21779	THKFHHRGRGYHSPNACLAG			20	SLAY-screened peptide P129			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCATAAGTTCCACCATCGGGGTCGCGGTTATCATTCTCCTAATGCTTGTCTTGCCGGCTAA	THKFHHRGRGYHSPNACLAG*	-4.452	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21780	NDLYIGLYELMVNPARDHPN			20	SLAY-screened peptide P130			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGACCTGTATATTGGGCTGTACGAGCTTATGGTTAATCCTGCTAGGGATCATCCTAATTAA	NDLYIGLYELMVNPARDHPN*	-4.447	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21781	ANLLLTLFMLTLRVGLAILSN			21	SLAY-screened peptide P131			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAACTTGCTGCTAACCCTATTCATGCTAACCTTACGTGTCGGGCTTGCTATACTATCTAAC	ANLLLTLFMLTLRVGLAILSN	-4.402	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21782	NPFLGSGSIGLFHRSMCCIL			20	SLAY-screened peptide P132			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCCGTTTCTTGGTAGCGGCTCTATCGGCCTGTTTCACAGGTCGATGTGCTGTATTTTGTAA	NPFLGSGSIGLFHRSMCCIL*	-4.385	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21783	RLHFGRGARVHVHYGMGAVH			20	SLAY-screened peptide P133			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCTTCATTTCGGCCGTGGTGCGCGCGTGCATGTCCATTATGGGATGGGTGCGGTCCACTAA	RLHFGRGARVHVHYGMGAVH*	-4.384	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21784	RLASNHNPHHLHTSHQE			17	SLAY-screened peptide P134			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTTGGCCTCTAATCACAATCCGCATCATCTGCATACTAGTCATCAGGAGTAACTGAGTAAG	RLASNHNPHHLHTSHQE*LSK	-4.38	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21785	LVDGSWYSRPYVHSAGPPRV			20	SLAY-screened peptide P135			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGTCGATGGTAGTTGGTATTCTCGGCCCTACGTTCATAGCGCCGGTCCGCCCCGGGTTTAA	LVDGSWYSRPYVHSAGPPRV*	-4.376	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21786	LAGACPLHNSPNNGF			15	SLAY-screened peptide P136			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGCTGGTGCCTGTCCTCTGCATAACTCTCCTAATAATGGTTTCTAGGTTTAGATCGTCTAA	LAGACPLHNSPNNGF*V*IV*	-4.374	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21787	SPISNTA			7	SLAY-screened peptide P137			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCCTATTAGTAATACTGCCTAGACGCGTTTTCCTCATCGTGACTGTAAGTGTGCGAATTAA	SPISNTA*TRFPHRDCKCAN*	-4.371	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21788	SPIHAHCCTTNYHDIIVDFV			20	SLAY-screened peptide P138			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCCGATCCATGCTCACTGTTGCACGACTAACTACCACGATATTATTGTTGATTTTGTTTAA	SPIHAHCCTTNYHDIIVDFV*	-4.366	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21789	RWALEPHSIWFHLKKMHLT			19	SLAY-screened peptide P139			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTGGGCGCTGGAGCCCCACTCTATCTGGTTTCACCTGAAGAAGATGCACCTCACTTAGTAA	RWALEPHSIWFHLKKMHLT**	-4.364	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21790	TVPRSERCRYCQLTDYLFSC			20	SLAY-screened peptide P140			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGTGCCTCGCTCTGAGCGCTGCCGGTACTGTCAGCTGACTGATTATTTGTTTTCGTGTTAA	TVPRSERCRYCQLTDYLFSC*	-4.358	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21791	PLG			3	SLAY-screened peptide P141			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTGGGTAGTATCTTGACAACCATTCTTATCGTTTCTATTGGTGCAAGACGACCCACTAA	PLG*YLDNHSYRFYWCKTTH*	-4.341	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21792	WSGVTHPNLLAILGIVCCLL			20	SLAY-screened peptide P142			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTCTGGTGTCACTCACCCTAATCTTCTCGCTATCCTTGGTATTGTTTGTTGCCTGCTTTAA	WSGVTHPNLLAILGIVCCLL*	-4.332	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21793	PH			2	SLAY-screened peptide P143			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCATTAGACCATTACGTATAATGATAACAAGAGCCTTATTCCGGCTACTTTGAATTCGTAA	PH*TITYNDNKSLIPATLNS*	-4.319	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21794	LA			2	SLAY-screened peptide P144			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGCTTAGCCGATTCACCTCTTCAATACTAATCACCCTAATATTGACTATTTTTATCTCTAA	LA*PIHLFNTNHPNIDYFYL*	-4.318	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21795	AIEVHAAWMLVPC			13	SLAY-screened peptide P145			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGATTGAGGTGCATGCGGCGTGGATGCTCGTTCCGTGCTAGACGGCGAATACGGCCAACTAA	AIEVHAAWMLVPC*TANTAN*	-4.285	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21796	QLDNHHLLHLNLRYGCRAYL			20	SLAY-screened peptide P146			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCTGGACAATCACCATCTGCTTCATCTTAACCTGCGTTATGGTTGCCGTGCCTATTTGTAA	QLDNHHLLHLNLRYGCRAYL*	-4.245	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21797	HWYYVHFRDHSSLYTLLPDL			20	SLAY-screened peptide P147			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGGTACTATGTTCATTTCCGTGACCATTCGAGTCTCTATACCCTGCTTCCTGACCTTTAA	HWYYVHFRDHSSLYTLLPDL*	-4.236	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21798	RAAFNRLTRFCAYVYSWQ			18	SLAY-screened peptide P148			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGCTGCTTTTAATCGCCTTACTCGTTTTTGCGCCTATGTTTATTCTTGGCAGTAGTAGTAA	RAAFNRLTRFCAYVYSWQ***	-4.223	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21799	SRFHPVVNAARPNAHEGYSA			20	SLAY-screened peptide P149			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCGGTTCCATCCTGTGGTTAACGCTGCTCGTCCTAATGCGCACGAGGGGTATAGCGCTTAA	SRFHPVVNAARPNAHEGYSA*	-4.211	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21800	LLTTPYSQLSNAVYLPCS			18	SLAY-screened peptide P150			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTCACTACTCCTTATTCCCAGTTGTCGAATGCGGTTTATTTGCCCTGCAGCTAGTTTTAA	LLTTPYSQLSNAVYLPCS*F*	-4.185	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21801	TPLRRHYSIRWLYVRIRRRN			20	SLAY-screened peptide P151			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCCCTTCGCCGGCATTACAGCATTCGTTGGCTGTACGTGCGTATTAGGCGTAGGAATTAA	TPLRRHYSIRWLYVRIRRRN*	-4.174	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21802	ATRRTATNLLGERTDAHTYR			20	SLAY-screened peptide P152			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTACTCGCCGGACTGCGACTAATCTTCTTGGTGAGCGTACTGATGCCCATACTTATAGGTAA	ATRRTATNLLGERTDAHTYR*	-4.169	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21803	AFTDDAVRIPGRRCTTFNCS			20	SLAY-screened peptide P153			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTTTACTGACGATGCTGTTCGGATTCCGGGTAGGCGTTGTACTACGTTCAACTGCAGCTAA	AFTDDAVRIPGRRCTTFNCS*	-4.164	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21804	TFPPVHLSSDAILGDLHHAG			20	SLAY-screened peptide P154			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTTTCCGCCGGTGCATCTGTCTTCTGACGCGATTTTGGGCGATCTTCACCATGCTGGTTAA	TFPPVHLSSDAILGDLHHAG*	-4.162	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21805	AMQIPNSLCAISS			13	SLAY-screened peptide P155			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTATGCAGATTCCCAACTCTCTTTGCGCTATTAGTTCTTAGAATAACCCTTATGGTCTTTAA	AMQIPNSLCAISS*NNPYGL*	-4.146	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21806	NVSLDNHGMLPGMLKSFYC			19	SLAY-screened peptide P156			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGTCTCCCTCGACAATCACGGTATGCTTCCTGGTATGCTTAAGAGCTTTTATTGCTAGTAA	NVSLDNHGMLPGMLKSFYC**	-4.126	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21807	AGGYRHYMYGPHDWRFHRFY			20	SLAY-screened peptide P157			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGGCGGCTATCGCCACTATATGTATGGTCCTCATGATTGGCGCTTTCATCGGTTTTATTAA	AGGYRHYMYGPHDWRFHRFY*	-4.125	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21808	MYNSASDETTSSHSNTGNYN			20	SLAY-screened peptide P158			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTATAATAGCGCCTCTGATGAGACTACCTCCTCTCATAGTAATACTGGTAATTATAATTAA	MYNSASDETTSSHSNTGNYN*	-4.12	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21809	CPFTVSDTSASYRSTRSFYS			20	SLAY-screened peptide P159			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCTTTCACTGTGTCTGATACCTCTGCGTCTTATCGTTCTACTCGTTCGTTTTATTCTTAA	CPFTVSDTSASYRSTRSFYS*	-4.113	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21810	LATSTLDYHSHLYSGPNSYG			20	SLAY-screened peptide P160			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGCGACGTCCACGCTGGACTATCACAGTCACTTGTACAGCGGGCCTAACAGCTACGGTTAA	LATSTLDYHSHLYSGPNSYG*	-4.093	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21811	THDLAHNNNYFRVGSYLRLY			20	SLAY-screened peptide P161			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCACGACTTGGCGCACAACAACAATTATTTTCGCGTCGGTAGTTATCTTCGTCTTTATTAA	THDLAHNNNYFRVGSYLRLY*	-4.084	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21812	MALWNPLLCKANHDLYLDAN			20	SLAY-screened peptide P162			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGCCTTGTGGAACCCTCTGCTGTGCAAGGCTAACCATGATCTGTATTTGGATGCTAACTAA	MALWNPLLCKANHDLYLDAN*	-4.062	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21813	KGVPVHIMPGAFFPSLVAGR			20	SLAY-screened peptide P163			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGGGTGTTCCTGTTCACATCATGCCTGGTGCTTTTTTCCCTTCTCTGGTTGCGGGCCGGTAA	KGVPVHIMPGAFFPSLVAGR*	-4.061	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21814	RLVSAEQHHNNSSYLAFMNE			20	SLAY-screened peptide P164			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCTTGTGTCTGCCGAGCAGCATCACAATAATAGTAGTTATCTGGCCTTTATGAATGAGTAA	RLVSAEQHHNNSSYLAFMNE*	-4.057	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21815	LVCLCDCFQPDRTGSSVSED			20	SLAY-screened peptide P165			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGTCTGCTTGTGTGATTGTTTCCAGCCCGATCGCACTGGTTCTAGTGTCTCTGAGGATTAA	LVCLCDCFQPDRTGSSVSED*	-4.035	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21816	TSNSPKALGNTASMSPMCHI			20	SLAY-screened peptide P166			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTCTAATTCTCCTAAGGCTCTGGGGAATACGGCCTCGATGAGCCCGATGTGCCATATTTAA	TSNSPKALGNTASMSPMCHI*	-4.022	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21817	NCAFERPNHPSPYYDFEYTI			20	SLAY-screened peptide P167			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTGCGCGTTTGAGCGTCCTAACCACCCTTCTCCGTATTATGACTTTGAGTATACTATTTAA	NCAFERPNHPSPYYDFEYTI*	-4.017	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21818	EPNHHSAVTGNRNNSATDND			20	SLAY-screened peptide P168			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCCCAATCATCACTCTGCTGTTACGGGTAATCGTAACAATAGCGCGACTGATAATGATTAA	EPNHHSAVTGNRNNSATDND*	-3.984	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21819	PDSPIVVVAQHKRPCDTLPF			20	SLAY-screened peptide P169			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGATAGTCCGATTGTGGTTGTTGCTCAGCATAAGCGTCCCTGTGATACGCTTCCTTTCTAA	PDSPIVVVAQHKRPCDTLPF*	-3.959	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21820	GHSPSLHCTMVIVIDGDNVT			20	SLAY-screened peptide P170			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCATTCTCCTTCTTTGCATTGTACGATGGTGATCGTTATTGATGGCGACAATGTCACTTAA	GHSPSLHCTMVIVIDGDNVT*	-3.949	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21821	PTRTEQWSTSNDSERTCLIL			20	SLAY-screened peptide P171			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACCCGGACGGAGCAGTGGAGTACTTCCAACGATAGTGAGCGGACGTGCCTCATTTTGTAA	PTRTEQWSTSNDSERTCLIL*	-3.936	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21822	CGHCHTCTIPYCGNLIVAIHY			21	SLAY-screened peptide P172			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGGCCATTGCCACACCTGCACCATTCCTTATTGCGGTAATCTTATTGTTGCTATCCACTAC	CGHCHTCTIPYCGNLIVAIHY	-3.921	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21823	PADTFFLLP			9	SLAY-screened peptide P173			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCTGATACTTTCTTTCTGCTTCCGTAGCCTCGCATTGATTCCACCCACCGTCAGGCGTAA	PADTFFLLP*PRIDSTHRQA*	-3.919	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21824	TVTSETLYFRLTCYTSRP			18	SLAY-screened peptide P174			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGTGACGAGTGAGACCCTTTACTTCAGGCTGACTTGCTATACGTCGCGTCCTTAGCCTTAA	TVTSETLYFRLTCYTSRP*P*	-3.91	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21825	PRSTDVARRPGLSTV			15	SLAY-screened peptide P175			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGGAGTACTGATGTGGCGCGGCGCCCTGGCCTCAGTACTGTTTAGACGCTTCAGACGTAA	PRSTDVARRPGLSTV*TLQT*	-3.901	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21826	TSPATYRHTNWRGAPPLPNT			20	SLAY-screened peptide P176			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTCTCCGGCTACTTACAGGCATACCAACTGGCGTGGTGCGCCGCCGCTCCCGAACACCTAA	TSPATYRHTNWRGAPPLPNT*	-3.884	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21827	SHLDNCTSVYNAANYTLMIG			20	SLAY-screened peptide P177			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCATCTCGATAATTGTACTAGTGTTTATAATGCCGCTAACTATACTTTGATGATTGGCTAA	SHLDNCTSVYNAANYTLMIG*	-3.882	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21828	ISSLR			5	SLAY-screened peptide P178			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTCGAGCTTGCGTTAGACTGAGATTCCTCCTGCGTGTGGCCACACTATTTCGTCGATGTAA	ISSLR*TEIPPACGHTISSM*	-3.88	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21829	LMYNATYDH			9	SLAY-screened peptide P179			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATGTACAATGCTACTTATGATCACTAGCCTAATACCAACCTTACCAATGCCATGAATTAA	LMYNATYDH*PNTNLTNAMN*	-3.873	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21830	TLHAGLYSVIIMFYGRWVSN			20	SLAY-screened peptide P180			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTGCATGCCGGCCTGTATTCTGTTATTATTATGTTTTACGGTCGTTGGGTTTCTAATTAA	TLHAGLYSVIIMFYGRWVSN*	-3.868	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21831	PAEWSTVVGNFTYHFNYNLL			20	SLAY-screened peptide P181			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCGGAGTGGTCGACTGTTGTGGGTAATTTTACGTACCATTTCAATTATAATCTCTTGTAA	PAEWSTVVGNFTYHFNYNLL*	-3.866	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21832	TLYIITYWDPDYKNIVSLTI			20	SLAY-screened peptide P182			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTTGTACATTATTACGTATTGGGATCCTGATTACAAGAATATTGTTTCGCTTACGATTTAA	TLYIITYWDPDYKNIVSLTI*	-3.866	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21833	HPITIPNLLLAYRMPVLMLF			20	SLAY-screened peptide P183			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCCATTACTATTCCTAATCTTCTTTTGGCTTACCGCATGCCTGTTCTCATGCTGTTTTAA	HPITIPNLLLAYRMPVLMLF*	-3.852	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21834	GDCTHKYADLPNAISNLFLR			20	SLAY-screened peptide P184			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTGATTGCACTCACAAGTATGCTGATCTCCCTAATGCTATCAGCAACCTTTTTTTGCGCTAA	GDCTHKYADLPNAISNLFLR*	-3.849	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21835	GHVAMDPNINAVFHTTADTS			20	SLAY-screened peptide P185			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCACGTGGCCATGGATCCCAACATCAATGCTGTCTTTCACACTACTGCTGATACTTCTTAA	GHVAMDPNINAVFHTTADTS*	-3.846	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21836	IKTPKLDPN			9	SLAY-screened peptide P186			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAAGACGCCTAAGCTTGATCCTAATTAGTGCATTTGCAAGATTCCTGTGCTTTACCGTTAA	IKTPKLDPN*CICKIPVLYR*	-3.846	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21837	HNFPCVYLARRRSSTRRGRVT			21	SLAY-screened peptide P187			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAACTTTCCGTGTGTCTACCTTGCCAGGCGCCGTTCATCAACTCGCCGAGGTCGAGTAACT	HNFPCVYLARRRSSTRRGRVT	-3.838	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21838	PSHGLDSSRRTHCNYIRTCE			20	SLAY-screened peptide P188			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGCCATGGTCTTGATTCTTCGCGTCGCACGCATTGCAACTATATTCGCACTTGTGAGTAA	PSHGLDSSRRTHCNYIRTCE*	-3.836	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21839	SVRGRCCSGEYARSRAVGTP			20	SLAY-screened peptide P189			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGTTCGTGGTCGGTGTTGTAGCGGTGAGTATGCGCGGTCTCGGGCTGTGGGGACCCCCTAA	SVRGRCCSGEYARSRAVGTP*	-3.831	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21840	PLQSPALATVMRIDHPTPTV			20	SLAY-screened peptide P190			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTGCAGAGTCCCGCTTTGGCGACGGTTATGCGTATCGACCACCCGACTCCGACCGTTTAA	PLQSPALATVMRIDHPTPTV*	-3.824	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21841	NICDTYILRDNRPFLT			16	SLAY-screened peptide P191			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATATTTGTGATACTTACATTCTGCGCGACAATCGTCCTTTCTTGACGTAGATCAGCATTAAC	NICDTYILRDNRPFLT*ISIN	-3.808	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21842	WPPPNYQRHDALKEEETSNL			20	SLAY-screened peptide P192			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCCCCCGCCGAATTACCAGCGCCACGATGCTCTCAAGGAGGAGGAGACGTCCAATTTGTAA	WPPPNYQRHDALKEEETSNL*	-3.803	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21843	VLLQY			5	SLAY-screened peptide P193			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCTTTTGCAGTACTAGCCTAAAATTAGGGCTGGAGCAGCTACGGTTACAACACTACTTAAC	VLLQY*PKIRAGAATVTTLLN	-3.795	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21844	PPGPANHAHHICIWPSEPAH			20	SLAY-screened peptide P194			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCGGGCCCTGCTAATCACGCTCACCATATTTGCATTTGGCCCTCGGAGCCCGCCCATTAA	PPGPANHAHHICIWPSEPAH*	-3.762	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21845	HVIASGCAVLLNYFRVMLPS			20	SLAY-screened peptide P195			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGTTATCGCCAGCGGTTGCGCTGTTTTGCTTAACTATTTTAGGGTTATGCTTCCTTCCTAA	HVIASGCAVLLNYFRVMLPS*	-3.755	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21846	DHHRFIAPDISLARYFILYT			20	SLAY-screened peptide P196			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCATCACCGTTTCATTGCCCCTGATATTTCTCTTGCTAGGTACTTTATTCTGTATACGTAA	DHHRFIAPDISLARYFILYT*	-3.753	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21847	PSSSQVPGDHFHFSNYVTFLY			21	SLAY-screened peptide P197			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGTTCCTCCCAGGTTCCCGGTGATCACTTTCACTTTAGCAATTATGTTACCTTCTTGTAC	PSSSQVPGDHFHFSNYVTFLY	-3.753	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21848	LFNLMSILNPDFSYYTNASN			20	SLAY-screened peptide P198			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTTTAACCTTATGTCTATTCTTAATCCTGACTTTAGCTACTATACTAACGCTTCTAATTAA	LFNLMSILNPDFSYYTNASN*	-3.748	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21849	STYF			4	SLAY-screened peptide P199			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTACTTACTTTTAGCTCTGTCACTATCTTCGTAACATTTCTCGGCAGAAGGGTGAGGCCTAA	STYF*LCHYLRNISRQKGEA*	-3.733	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21850	SNSCLSSPCNIHYSVIPDRN			20	SLAY-screened peptide P200			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGAATTCTTGCCTTTCTAGTCCTTGTAACATTCATTATAGTGTCATCCCTGATAGGAACTAA	SNSCLSSPCNIHYSVIPDRN*	-3.722	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21851	MQARPHWFSHDL			12	SLAY-screened peptide P201			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCAGGCTCGCCCCCACTGGTTCTCGCATGACCTGTAGTAGTGTAACTTGGATTATTATTAA	MQARPHWFSHDL**CNLDYY*	-3.697	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21852	NAPIISVCYCSTQILCLGDI			20	SLAY-screened peptide P202			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGCGCCTATTATCAGTGTCTGTTATTGCAGTACTCAGATTCTCTGCCTTGGTGATATTTAA	NAPIISVCYCSTQILCLGDI*	-3.692	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21853	NRPNM			5	SLAY-screened peptide P203			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCGTCCGAACATGTAGAAGTGCGGCACTTTGCCGCCTCGTTTTTAGGTTGCTATTGTCTAA	NRPNM*KCGTLPPRF*VAIV*	-3.684	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21854	PMHYLGSTTLKKNHLYHDSIN			21	SLAY-screened peptide P204			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATGCATTACCTCGGTTCTACTACTCTTAAGAAGAATCATCTGTACCATGACTCGATTAAC	PMHYLGSTTLKKNHLYHDSIN	-3.683	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21855	ASDSPFQECDHLFYISNYIL			20	SLAY-screened peptide P205			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGAGTGATTCTCCTTTCCAGGAGTGTGACCATCTCTTTTACATTTCTAATTATATCCTGTAA	ASDSPFQECDHLFYISNYIL*	-3.682	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21856	RPFSKHSYNTDNTDYYHSNC			20	SLAY-screened peptide P206			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCCTTTTCTAAGCACTCCTACAACACGGATAATACTGATTATTATCATTCTAACTGCTAA	RPFSKHSYNTDNTDYYHSNC*	-3.675	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21857	LNMLLYSTFRFTCSGNDHYH			20	SLAY-screened peptide P207			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAACATGCTTCTTTACTCGACTTTTAGGTTTACGTGTTCGGGTAACGATCACTATCACTAA	LNMLLYSTFRFTCSGNDHYH*	-3.673	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21858	LLVSYCNGDIKHCHPNNSFS			20	SLAY-screened peptide P208			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTCGTGTCTTATTGCAATGGTGACATTAAGCATTGCCACCCTAATAATTCGTTTTCTTAA	LLVSYCNGDIKHCHPNNSFS*	-3.656	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21859	PLCYTLHSHNYNAAYYSSLS			20	SLAY-screened peptide P209			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTTTGTTACACGCTGCACTCCCATAATTATAATGCTGCTTATTATTCGAGTCTGTCGTAA	PLCYTLHSHNYNAAYYSSLS*	-3.656	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21860	PKTPASYCTIIVMVDNTVSL			20	SLAY-screened peptide P210			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAAGACGCCCGCGTCTTACTGTACGATTATCGTTATGGTTGACAATACTGTTTCTCTGTAA	PKTPASYCTIIVMVDNTVSL*	-3.653	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21861	LFHSEFSDTQRSIHNISDYL			20	SLAY-screened peptide P211			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTTCATTCTGAGTTTAGTGATACTCAGAGGTCCATTCACAACATCAGCGATTACTTGTAA	LFHSEFSDTQRSIHNISDYL*	-3.648	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21862	WTSRFALLTKHFAIFVTILTN			21	SLAY-screened peptide P212			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGACTTCCCGCTTTGCGCTCCTTACTAAGCACTTCGCTATCTTTGTAACCATCCTAACTAAC	WTSRFALLTKHFAIFVTILTN	-3.633	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21863	HHRNLQLYTFLSLLWTHYAA			20	SLAY-screened peptide P213			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCACCGTAATCTGCAGTTGTATACGTTCCTGTCTTTGCTGTGGACTCATTACGCCGCGTAA	HHRNLQLYTFLSLLWTHYAA*	-3.633	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21864	PYARSLGTGGNYIVNIIPRY			20	SLAY-screened peptide P214			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTATGCCCGGAGCCTTGGCACTGGGGGTAATTATATTGTTAACATTATTCCTCGTTATTAA	PYARSLGTGGNYIVNIIPRY*	-3.631	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21865	SSNRHLGGSCQSPESDNYSIY			21	SLAY-screened peptide P215			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTCTAACCGTCACTTGGGTGGTAGCTGTCAGAGTCCCGAGAGCGATAACTATAGTATTTAC	SSNRHLGGSCQSPESDNYSIY	-3.628	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21866	SDD			3	SLAY-screened peptide P216			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGACGATTAGTATACGTGTATGCCGATTCCCACGTATTTCCGTGTCAATCTCTTCCCCTAA	SDD*YTCMPIPTYFRVNLFP*	-3.623	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21867	PPCCVTPPSILSFAVATCAT			20	SLAY-screened peptide P217			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCTTGCTGCGTTACCCCGCCTAGCATCCTTAGTTTTGCTGTGGCTACGTGCGCGACGTAA	PPCCVTPPSILSFAVATCAT*	-3.612	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21868	ILVVSL			6	SLAY-screened peptide P218			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCTCGTCGTCTCGTTGTAGCATCCTGGCGCCTATCCCTCCATGCTGTGGTCTACCACTTAA	ILVVSL*HPGAYPSMLWSTT*	-3.596	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21869	PNLHSGNRPLYNLFAYAAHG			20	SLAY-screened peptide P219			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAATCTTCACTCTGGTAATAGGCCGCTCTATAATCTGTTTGCCTACGCTGCCCATGGTTAA	PNLHSGNRPLYNLFAYAAHG*	-3.591	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21870	HYFYLDVLAILPLHFKSIPC			20	SLAY-screened peptide P220			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTACTTCTATCTGGACGTGCTTGCTATTCTCCCGCTTCATTTTAAGAGTATTCCTTGTTAA	HYFYLDVLAILPLHFKSIPC*	-3.587	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21871	RDIITIRHCAYRHTPNTRIC			20	SLAY-screened peptide P221			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGATATTATCACGATCAGGCACTGTGCGTATCGCCATACGCCTAACACTCGCATTTGCTAA	RDIITIRHCAYRHTPNTRIC*	-3.579	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21872	RHIAYHCNMLFSDFLDRFLE			20	SLAY-screened peptide P222			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCATATTGCTTATCATTGCAACATGCTGTTTTCTGACTTTCTCGATCGTTTTCTCGAGTAA	RHIAYHCNMLFSDFLDRFLE*	-3.576	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21873	YSGTYTGFSNYCIVDCTI			18	SLAY-screened peptide P223			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAGTGGCACGTATACTGGTTTCTCTAATTATTGCATTGTGGATTGTACCATCTAGTATTAA	YSGTYTGFSNYCIVDCTI*Y*	-3.574	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21874	PPYNLHTDN			9	SLAY-screened peptide P224			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCTTATAACCTTCACACTGATAATTAGTCTTTTCGGGACGAGTATCTTAAGTCTAGGTAA	PPYNLHTDN*SFRDEYLKSR*	-3.573	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21875	AWNYNYGKPPLGINLQYLRT			20	SLAY-screened peptide P225			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTGGAATTACAATTACGGCAAGCCTCCTCTGGGTATCAACCTGCAGTATCTCCGGACCTAA	AWNYNYGKPPLGINLQYLRT*	-3.572	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21876	RPFHTTPNFSRCLYPRDSFL			20	SLAY-screened peptide P226			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCTTTTCATACTACGCCTAACTTTAGCCGTTGTCTGTATCCGCGTGATTCTTTTCTCTAA	RPFHTTPNFSRCLYPRDSFL*	-3.571	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21877	PFGKHRSGLFPRHNSKTAQL			20	SLAY-screened peptide P227			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTTGGGAAGCATCGTTCTGGTCTTTTTCCTAGGCATAACAGCAAGACCGCGCAGCTGTAA	PFGKHRSGLFPRHNSKTAQL*	-3.569	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21878	PGGCPSLRMHDLDDTMHVLQ			20	SLAY-screened peptide P228			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGTGGCTGTCCTAGTCTTCGCATGCACGATCTCGATGATACTATGCACGTTCTTCAGTAA	PGGCPSLRMHDLDDTMHVLQ*	-3.565	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21879	RGSKDCAYPASSNLDSIILN			20	SLAY-screened peptide P229			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGGTTCGAAGGACTGTGCTTACCCTGCTTCTTCTAATTTGGATTCCATTATTCTGAACTAA	RGSKDCAYPASSNLDSIILN*	-3.556	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21880	PILCDLGVAYAIPPFCDD			18	SLAY-screened peptide P230			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATCCTTTGTGACCTGGGTGTTGCTTATGCGATTCCGCCTTTCTGTGATGATTAGACCTAA	PILCDLGVAYAIPPFCDD*T*	-3.553	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21881	RRARGVYTWYSNLPSAQRVP			20	SLAY-screened peptide P231			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCGCGCTCGGGGCGTGTATACCTGGTATTCTAACCTTCCGTCGGCCCAGCGGGTTCCCTAA	RRARGVYTWYSNLPSAQRVP*	-3.543	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21882	RTLTFMVRIGAKMLFFEIRY			20	SLAY-screened peptide P232			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACCCTCACCTTTATGGTTCGCATTGGGGCCAAGATGCTCTTTTTTGAGATTAGGTATTAA	RTLTFMVRIGAKMLFFEIRY*	-3.537	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21883	RMGSSYTSGIDLWLVLHHNN			20	SLAY-screened peptide P233			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGATGGGGTCTTCCTACACTTCTGGTATTGACCTGTGGCTGGTGCTGCATCATAATAATTAA	RMGSSYTSGIDLWLVLHHNN*	-3.535	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21884	PYWLGTLDRVNYLGPTGYAF			20	SLAY-screened peptide P234			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTATTGGCTGGGTACTCTTGATCGCGTCAATTACCTTGGCCCCACGGGGTATGCCTTCTAA	PYWLGTLDRVNYLGPTGYAF*	-3.53	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21885	PGPYSKSLLSIRCADPN			17	SLAY-screened peptide P235			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGGCCCTTACTCTAAGTCTCTGCTTTCTATTCGGTGTGCTGACCCTAACTAGAACGCTTAA	PGPYSKSLLSIRCADPN*NA*	-3.523	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21886	YRNTTTIR			8	SLAY-screened peptide P236			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCGTAACACTACGACTATTCGTTAGGATCTCCATAGCAACGGCGACTCTAGTAGCCCTTAA	YRNTTTIR*DLHSNGDSSSP*	-3.521	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21887	SQTYCIWLRVRIRIAIIIRLN			21	SLAY-screened peptide P237			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCAGACTTACTGCATTTGGTTACGCGTACGAATCCGCATAGCAATAATTATACGACTTAAC	SQTYCIWLRVRIRIAIIIRLN	-3.517	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21888	TNFHMLNYYASPGCSYKEPL			20	SLAY-screened peptide P238			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAATTTCCATATGCTTAATTATTACGCCAGCCCGGGCTGCTCTTATAAGGAGCCCCTCTAA	TNFHMLNYYASPGCSYKEPL*	-3.514	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21889	RSNNVFTLPQNLHSANKLCP			20	SLAY-screened peptide P239			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTCGAATAATGTTTTTACGCTTCCTCAGAATCTTCATTCGGCTAATAAGCTCTGCCCTTAA	RSNNVFTLPQNLHSANKLCP*	-3.511	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21890	VGTSSLNGDKVPNLPRRVIR			20	SLAY-screened peptide P240			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGGGACGTCTAGCCTTAATGGCGACAAGGTTCCTAATTTGCCTAGGAGGGTCATTCGTTAA	VGTSSLNGDKVPNLPRRVIR*	-3.506	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21891	VSVILRPNGLNLSVRLSYAC			20	SLAY-screened peptide P241			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTCTGTTATTCTTAGGCCTAACGGTCTTAACCTTTCTGTTCGTCTGAGCTACGCTTGCTAA	VSVILRPNGLNLSVRLSYAC*	-3.504	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21892	PPGGYHCDLYFLILRH			16	SLAY-screened peptide P242			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCGGGCGGGTATCATTGTGATTTGTATTTTCTTATTCTTCGTCACTAGCAGAAGTAGTAA	PPGGYHCDLYFLILRH*QK**	-3.497	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21893	RLALTFIHRLYHPNHLNFHS			20	SLAY-screened peptide P243			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTGGCGCTCACGTTCATCCACAGGCTTTATCATCCTAATCACCTTAACTTTCACTCTTAA	RLALTFIHRLYHPNHLNFHS*	-3.489	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21894	IRYGPPCSHNLREHLPKTLE			20	SLAY-screened peptide P244			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAGGTATGGCCCGCCTTGTAGCCACAATCTTCGGGAGCATCTTCCGAAGACCCTCGAGTAA	IRYGPPCSHNLREHLPKTLE*	-3.478	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21895	GNLNFPIEWKARRMVEVKSQ			20	SLAY-screened peptide P245			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGAATCTTAATTTTCCTATTGAGTGGAAGGCGCGTCGTATGGTTGAGGTCAAGTCCCAGTAA	GNLNFPIEWKARRMVEVKSQ*	-3.477	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21896	DIHAITRVPDTQLIHFVCIS			20	SLAY-screened peptide P246			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATATTCATGCGATCACTCGTGTCCCTGATACGCAGCTTATCCATTTTGTCTGCATTTCTTAA	DIHAITRVPDTQLIHFVCIS*	-3.471	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21897	QSTSNLHMSYTVNGTNVLGR			20	SLAY-screened peptide P247			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTCCACTTCGAATCTCCATATGTCTTACACTGTCAACGGGACTAATGTCCTGGGGCGTTAA	QSTSNLHMSYTVNGTNVLGR*	-3.469	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21898	NSVDPIDSDIDMTYNALHSDY			21	SLAY-screened peptide P248			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTCGGTTGATCCTATTGATTCTGACATTGATATGACTTATAATGCCTTGCATAGTGATTAC	NSVDPIDSDIDMTYNALHSDY	-3.465	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21899	PPVRKRITVSYHILFNKNND			20	SLAY-screened peptide P249			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCCGTCCGTAAGCGTATCACCGTTAGTTATCATATTTTGTTTAATAAGAATAACGACTAA	PPVRKRITVSYHILFNKNND*	-3.454	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21900	PSDLVPTLSPNNRGPPEYSP			20	SLAY-screened peptide P250			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCGGACCTGGTTCCCACTCTTTCTCCGAACAACCGCGGCCCGCCGGAGTACTCTCCCTAA	PSDLVPTLSPNNRGPPEYSP*	-3.453	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21901	TCMHNNWLPLATLSDRRHLF			20	SLAY-screened peptide P251			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTGTATGCATAATAATTGGCTTCCGCTTGCGACCCTGAGCGACAGGAGGCATTTGTTTTAA	TCMHNNWLPLATLSDRRHLF*	-3.452	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21902	TGRGRHPHGTRTVRIATPNN			20	SLAY-screened peptide P252			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGGCCGGGGTAGGCACCCTCACGGCACTCGTACGGTTCGTATTGCTACGCCGAACAATTAA	TGRGRHPHGTRTVRIATPNN*	-3.45	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21903	STPGLCTTASPPFVP			15	SLAY-screened peptide P253			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGACCCCCGGTCTTTGCACCACCGCGAGTCCTCCTTTCGTGCCGTAGACGATCTACTACTAA	STPGLCTTASPPFVP*TIYY*	-3.449	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21904	CPPLLGYSARDRLSIYGSIV			20	SLAY-screened peptide P254			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCTCCTCTCCTGGGGTACTCCGCTAGGGACCGTCTCAGTATTTATGGTTCGATTGTGTAA	CPPLLGYSARDRLSIYGSIV*	-3.447	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21905	SSYNAHMM			8	SLAY-screened peptide P255			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCAGTTACAATGCTCATATGATGTAGGGTTGGCATAGCACCATCAATACTTTTAAGTGTTAA	SSYNAHMM*GWHSTINTFKC*	-3.447	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21906	ANSLCFIRGPPSFISKLHNI			20	SLAY-screened peptide P256			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAACTCGCTCTGTTTTATCCGGGGTCCGCCGTCCTTTATCAGCAAGCTTCATAATATTTAA	ANSLCFIRGPPSFISKLHNI*	-3.446	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21907	KCCSPDTCPTVPEIHMPLSS			20	SLAY-screened peptide P257			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGTGCTGTAGTCCTGACACTTGCCCTACGGTTCCTGAGATTCATATGCCTCTCTCGAGTTAA	KCCSPDTCPTVPEIHMPLSS*	-3.444	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21908	HYHRFATGATRSSYHTHAFI			20	SLAY-screened peptide P258			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTACCATCGCTTCGCCACGGGTGCTACGCGCAGCTCTTACCATACTCATGCGTTTATTTAA	HYHRFATGATRSSYHTHAFI*	-3.444	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21909	TSVDPNICICILFGHLSGYY			20	SLAY-screened peptide P259			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTCCGTGGATCCTAACATTTGTATCTGTATCCTTTTTGGTCATCTCAGTGGTTACTATTAA	TSVDPNICICILFGHLSGYY*	-3.443	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21910	CPTSALPSSGLLTVPTYASS			20	SLAY-screened peptide P260			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCCACCTCGGCGCTCCCGTCCTCGGGGCTTCTCACTGTGCCTACTTACGCGTCGAGTTAA	CPTSALPSSGLLTVPTYASS*	-3.44	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21911	TYTQ			4	SLAY-screened peptide P261			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTACACGCAGTAGAAGCATTGGCAGGACCCGCACGCGGCTACGACTTCGTCCGAGAATTAA	TYTQ*KHWQDPHAATTSSEN*	-3.423	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21912	DTLFHPKLHPHSAPTCTM			18	SLAY-screened peptide P262			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATACCTTGTTTCACCCCAAGCTGCATCCCCATTCCGCCCCTACTTGCACCATGTAGTTCTAA	DTLFHPKLHPHSAPTCTM*F*	-3.421	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21913	YPIRHSLPYAPYMFRTVACP			20	SLAY-screened peptide P263			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCCTATTAGGCATTCTCTTCCTTACGCTCCGTATATGTTCCGCACTGTCGCTTGCCCGTAA	YPIRHSLPYAPYMFRTVACP*	-3.421	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21914	PC			2	SLAY-screened peptide P264			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGCTAGGGTGACGGGTCGAGTATTAACAGGTATAGCGCCCCGGCGTAGGCGTTCCACTAA	PC*GDGSSINRYSAPA*AFH*	-3.42	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21915	ATCEFWRECT			10	SLAY-screened peptide P265			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTACTTGTGAGTTCTGGAGGGAGTGCACCTAGCGGGCTTACGTTTACTCTGGTATTCTTTAA	ATCEFWRECT*RAYVYSGIL*	-3.416	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21916	MLCPHYSGHSRYTVRTFCKN			20	SLAY-screened peptide P266			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCTTTGTCCGCACTACTCTGGCCACAGTAGGTATACGGTTCGTACTTTTTGTAAGAACTAA	MLCPHYSGHSRYTVRTFCKN*	-3.415	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21917	LIILCYTTRSSIDTKYVPS			19	SLAY-screened peptide P267			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATTATCCTTTGTTACACTACTCGTTCTAGTATCGACACGAAGTATGTTCCGTCCTAGTAA	LIILCYTTRSSIDTKYVPS**	-3.412	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21918	GVVCYLATDSPGTYPGSLSL			20	SLAY-screened peptide P268			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGTCGTGTGCTATCTTGCGACTGATTCGCCGGGCACTTATCCTGGGTCTCTCAGTTTGTAA	GVVCYLATDSPGTYPGSLSL*	-3.408	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21919	ELWPYFPSSYDLLCMPVDTY			20	SLAY-screened peptide P269			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCTCTGGCCCTACTTTCCGTCCTCCTACGATCTCCTCTGCATGCCTGTGGACACCTACTAA	ELWPYFPSSYDLLCMPVDTY*	-3.402	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21920	AGRNFPNCLCGLDAMTSSDI			20	SLAY-screened peptide P270			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGGGAGGAATTTCCCTAACTGCCTCTGTGGGCTGGATGCTATGACGAGTTCTGACATCTAA	AGRNFPNCLCGLDAMTSSDI*	-3.399	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21921	IPPQCPGILLPAYAFSVDSI			20	SLAY-screened peptide P271			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCCGCCTCAGTGCCCTGGTATCCTGCTTCCGGCGTATGCTTTTTCCGTTGACAGTATTTAA	IPPQCPGILLPAYAFSVDSI*	-3.391	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21922	RVHAVPPPGSHFPFLTRAGCN			21	SLAY-screened peptide P272			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGTTCATGCGGTTCCGCCGCCGGGCTCTCACTTTCCCTTCCTCACGCGGGCAGGATGTAAC	RVHAVPPPGSHFPFLTRAGCN	-3.387	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21923	HAGMDSADFIEYSASNKAHL			20	SLAY-screened peptide P273			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGCTGGCATGGATTCTGCGGATTTTATCGAGTATTCGGCGAGTAACAAGGCTCATCTTTAA	HAGMDSADFIEYSASNKAHL*	-3.375	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21924	SSFNWCPHRVFFCLSTKEVP			20	SLAY-screened peptide P274			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTCTTTCAATTGGTGTCCGCATCGGGTTTTTTTCTGTCTTTCGACCAAGGAGGTCCCTTAA	SSFNWCPHRVFFCLSTKEVP*	-3.371	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21925	DICDNNIETNFQWTTDV			17	SLAY-screened peptide P275			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATATTTGTGATAATAATATTGAGACCAACTTTCAGTGGACCACTGACGTTTAGCTCCCTTAA	DICDNNIETNFQWTTDV*LP*	-3.368	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21926	QSTTLHTTCGYMSNENDEKG			20	SLAY-screened peptide P276			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGAGTACTACCTTGCATACTACTTGTGGTTATATGTCTAACGAGAATGACGAGAAGGGTTAA	QSTTLHTTCGYMSNENDEKG*	-3.363	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21927	FHILIARFRRARRFTIAVVIN			21	SLAY-screened peptide P277			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCACATCCTCATAGCCAGGTTCCGTCGAGCCCGTCGCTTCACGATTGCCGTCGTGATTAAC	FHILIARFRRARRFTIAVVIN	-3.355	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21928	SYYRIHQRIIVSSINAFTNY			20	SLAY-screened peptide P278			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTATTATCGTATCCACCAGCGGATCATTGTTTCGTCTATCAATGCTTTTACTAATTATTAA	SYYRIHQRIIVSSINAFTNY*	-3.342	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21929	PSPDLRAPSNHYNVYGTSH			19	SLAY-screened peptide P279			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCGCCCGACCTCAGGGCTCCTTCTAATCATTATAATGTTTACGGTACCTCGCACTAGTAA	PSPDLRAPSNHYNVYGTSH**	-3.339	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21930	ANCTHAIYNNFCHHDHAYRT			20	SLAY-screened peptide P280			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAACTGTACCCATGCGATTTATAATAATTTCTGTCATCACGATCACGCCTACCGTACGTAA	ANCTHAIYNNFCHHDHAYRT*	-3.336	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21931	FLYHNFAMGWFIPGRPMYRA			20	SLAY-screened peptide P281			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCTCTACCACAACTTTGCTATGGGTTGGTTCATCCCCGGGCGCCCGATGTATAGGGCTTAA	FLYHNFAMGWFIPGRPMYRA*	-3.308	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21932	LMRTVGADSLSALFPDMGKP			20	SLAY-screened peptide P282			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCATGCGTACGGTCGGTGCTGATTCGCTGTCTGCTCTTTTTCCGGACATGGGCAAGCCCTAA	LMRTVGADSLSALFPDMGKP*	-3.299	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21933	EDPAPYTYIPHRYSSSISTH			20	SLAY-screened peptide P283			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGACCCCGCCCCTTACACTTATATTCCTCATAGGTATTCGAGTTCTATCTCGACCCATTAA	EDPAPYTYIPHRYSSSISTH*	-3.299	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21934	GI			2	SLAY-screened peptide P284			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTATTTAGAACCACGCTTTCTGGGTGCACAGTTATGTTCCGGGGAGGTAGACGGACACGTAA	GI*NHAFWVHSYVPGR*TDT*	-3.297	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21935	HSLSLSIRDSHINYECNNDS			20	SLAY-screened peptide P285			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAGTTTGTCGCTTTCCATTAGGGATTCTCATATCAATTATGAGTGCAATAACGATTCGTAA	HSLSLSIRDSHINYECNNDS*	-3.297	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21936	QNLITNFVGGNERHILPIAF			20	SLAY-screened peptide P286			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGAATCTCATTACTAATTTCGTTGGGGGTAACGAGCGGCATATCTTGCCCATTGCCTTTTAA	QNLITNFVGGNERHILPIAF*	-3.296	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21937	HANFSAPLTFLLTIRRRARG			20	SLAY-screened peptide P287			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCCAACTTCAGTGCCCCTCTCACTTTTTTGCTCACGATTCGCCGGCGGGCTAGGGGCTAA	HANFSAPLTFLLTIRRRARG*	-3.294	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21938	TITPAIPLPRIRSPPSCTFVT			21	SLAY-screened peptide P288			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCATTACTCCAGCGATCCCCTTGCCTCGAATTAGATCGCCACCAAGCTGTACATTCGTAACT	TITPAIPLPRIRSPPSCTFVT	-3.293	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21939	PNRQHLNFHYFCLMLHPPMP			20	SLAY-screened peptide P289			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAACCGTCAGCATCTTAATTTTCATTATTTCTGCCTTATGCTCCATCCGCCCATGCCGTAA	PNRQHLNFHYFCLMLHPPMP*	-3.293	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21940	QGNHTLNPSFSANNSFCAIT			20	SLAY-screened peptide P290			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGGCAATCATACTCTTAATCCTAGCTTTTCCGCCAATAATAGCTTTTGCGCTATTACCTAA	QGNHTLNPSFSANNSFCAIT*	-3.29	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21941	LHSTSIHAMSSHSRTINGKH			20	SLAY-screened peptide P291			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCATAGCACTTCTATCCATGCTATGTCTAGCCATAGTCGTACTATCAACGGCAAGCACTAA	LHSTSIHAMSSHSRTINGKH*	-3.282	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21942	IFDHDTHCSCNFHFIANGSW			20	SLAY-screened peptide P292			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTTTCGATCATGATACTCACTGTAGTTGCAATTTTCACTTTATCGCGAACGGCTCTTGGTAA	IFDHDTHCSCNFHFIANGSW*	-3.281	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21943	RKCVISVARRNRRANIKILCN			21	SLAY-screened peptide P293			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAAGTGCGTTATCTCCGTGGCGCGCCGTAATCGTAGGGCCAACATCAAGATCTTATGTAAC	RKCVISVARRNRRANIKILCN	-3.271	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21944	EPTILPDSDNSWIYTLDFTK			20	SLAY-screened peptide P294			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCCCACTATTCTCCCCGACTCCGATAATTCTTGGATTTATACTCTTGATTTTACTAAGTAA	EPTILPDSDNSWIYTLDFTK*	-3.266	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21945	HNSFDSLLFYRKMDECVVGA			20	SLAY-screened peptide P295			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAACTCTTTTGATTCGCTGCTCTTTTACCGTAAGATGGACGAGTGTGTTGTTGGGGCCTAA	HNSFDSLLFYRKMDECVVGA*	-3.262	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21946	LSMQ			4	SLAY-screened peptide P296			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCGATGCAGTAGTTCCCGGATCTTAGTCCGCGGTTGCGTTCGCATAGTGATACGTAACTG	LSMQ*FPDLSPRLRSHSDT*L	-3.254	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21947	IEAPSTVPPYPFTQSCYESW			20	SLAY-screened peptide P297			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGAGGCGCCCTCTACGGTTCCTCCTTATCCGTTTACTCAGAGCTGTTACGAGAGCTGGTAA	IEAPSTVPPYPFTQSCYESW*	-3.25	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21948	ISANYRSVFDSQHRVNDLLA			20	SLAY-screened peptide P298			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAGCGCGAACTATCGCAGTGTCTTTGATAGCCAGCATCGTGTCAATGATCTTCTCGCCTAA	ISANYRSVFDSQHRVNDLLA*	-3.246	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21949	LAMPFIKYPLNSRDGVCTHP			20	SLAY-screened peptide P299			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGCTATGCCGTTTATTAAGTACCCGTTGAACAGCCGTGACGGCGTTTGCACGCACCCCTAA	LAMPFIKYPLNSRDGVCTHP*	-3.246	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21950	ILLRNTGFITRVFQTCVEPV			20	SLAY-screened peptide P300			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCTCCTTCGCAATACCGGTTTTATTACTCGGGTGTTCCAGACTTGCGTTGAGCCCGTTTAA	ILLRNTGFITRVFQTCVEPV*	-3.246	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21951	FTMYVVLLHIRQNL			14	SLAY-screened peptide P301			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTACCATGTACGTGGTCCTTCTCCATATTCGCCAGAATCTCTAGGCCCCGGACGCCTGTTAA	FTMYVVLLHIRQNL*APDAC*	-3.244	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21952	SLDYNHRIDLSVLPYCLGPT			20	SLAY-screened peptide P302			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCTCGATTATAATCACCGTATCGACCTTTCTGTTCTTCCGTACTGCCTCGGTCCGACCTAA	SLDYNHRIDLSVLPYCLGPT*	-3.243	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21953	PRPRWPPPTTHTIVTPQDTL			20	SLAY-screened peptide P303			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGGCCGAGGTGGCCCCCTCCCACTACTCACACTATTGTTACTCCCCAGGACACGTTGTAA	PRPRWPPPTTHTIVTPQDTL*	-3.24	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21954	RLHATTYMHMHRDLMNFAFL			20	SLAY-screened peptide P304			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCTGCATGCTACCACGTATATGCATATGCATCGTGACTTGATGAACTTCGCGTTCCTGTAA	RLHATTYMHMHRDLMNFAFL*	-3.239	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21955	APYRRCSKNRLVLAS			15	SLAY-screened peptide P305			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCCCTATCGGCGCTGCTCTAAGAACCGCTTGGTTCTGGCTTCCTAGCAGACGACGTAGTAA	APYRRCSKNRLVLAS*QTT**	-3.23	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21956	PRRAYFNFNGGSYDTVTISF			20	SLAY-screened peptide P306			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGGAGGGCGTACTTCAACTTCAACGGCGGTAGTTACGATACGGTCACTATTAGTTTCTAA	PRRAYFNFNGGSYDTVTISF*	-3.218	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21957	LGEAYECSTFNFGST			15	SLAY-screened peptide P307			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGGCGAGGCTTATGAGTGCAGCACTTTCAATTTTGGCTCGACTTAGCACACTGTCGCTAAC	LGEAYECSTFNFGST*HTVAN	-3.209	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21958	VITPDRSGHFTFDHYYYWAS			20	SLAY-screened peptide P308			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCATCACGCCGGACCGTTCCGGCCACTTTACGTTCGATCACTATTATTACTGGGCCAGTTAA	VITPDRSGHFTFDHYYYWAS*	-3.206	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21959	IGHLYHSYVSSCSRSGVGMS			20	SLAY-screened peptide P309			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGGTCATCTTTACCATAGCTACGTGTCTAGCTGCTCTAGGTCCGGTGTGGGTATGTCTTAA	IGHLYHSYVSSCSRSGVGMS*	-3.204	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21960	NAWYTVHYTHNFVIS			15	SLAY-screened peptide P310			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGCGTGGTATACTGTTCACTACACTCATAATTTTGTCATCAGCTAGGACCATACGCAGTAA	NAWYTVHYTHNFVIS*DHTQ*	-3.202	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21961	DFLALSHYTCCSSNHIPPCH			20	SLAY-screened peptide P311			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTTTTTGGCGCTCAGTCACTATACGTGTTGCTCTTCTAATCATATCCCTCCTTGTCACTAA	DFLALSHYTCCSSNHIPPCH*	-3.202	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21962	YSTMFHDHPGMGGFDRPPQL			20	SLAY-screened peptide P312			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTCTACCATGTTCCATGATCACCCCGGTATGGGTGGTTTTGATCGTCCGCCCCAGCTGTAA	YSTMFHDHPGMGGFDRPPQL*	-3.201	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21963	MQPHRRNYNTYSLFTDPSDT			20	SLAY-screened peptide P313			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCAGCCTCACCGCCGCAATTATAATACGTATAGTCTTTTTACTGACCCTAGCGATACCTAA	MQPHRRNYNTYSLFTDPSDT*	-3.198	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21964	HTVLPLYRTVTSKCSHTMGV			20	SLAY-screened peptide P314			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACTGTCCTTCCTCTGTATCGGACCGTCACTTCTAAGTGCTCTCACACTATGGGTGTCTAA	HTVLPLYRTVTSKCSHTMGV*	-3.194	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21965	PYNVYHSFKHYHIYDDNWVP			20	SLAY-screened peptide P315			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTACAACGTCTATCACAGTTTTAAGCATTACCATATCTATGACGACAATTGGGTGCCTTAA	PYNVYHSFKHYHIYDDNWVP*	-3.194	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21966	CLHCLCYSGSDCDNIYSFIS			20	SLAY-screened peptide P316			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTTGCATTGTTTGTGCTATTCTGGGAGTGACTGCGACAATATTTACTCTTTCATTTCCTAA	CLHCLCYSGSDCDNIYSFIS*	-3.192	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21967	LHAIFLHCCKIHAQCVTLYT			20	SLAY-screened peptide P317			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCACGCCATTTTCCTGCATTGTTGCAAGATCCACGCTCAGTGCGTCACTTTGTATACTTAA	LHAIFLHCCKIHAQCVTLYT*	-3.19	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21968	PALHYVNFERYMPSDNRRL			19	SLAY-screened peptide P318			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCTTTGCATTACGTTAACTTCGAGCGGTATATGCCTTCGGACAATCGCCGGCTGTAGTAA	PALHYVNFERYMPSDNRRL**	-3.183	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21969	NLSLPDYNICMHREHPTILL			20	SLAY-screened peptide P319			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCTGTCGCTTCCCGATTATAACATCTGCATGCACCGCGAGCACCCTACCATTCTCCTGTAA	NLSLPDYNICMHREHPTILL*	-3.181	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21970	MRFNPTHIYSVPLMTLAPLIN			21	SLAY-screened peptide P320			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCGCTTTAACCCTACTCATATTTATAGCGTACCGTTGATGACACTGGCACCTCTAATTAAC	MRFNPTHIYSVPLMTLAPLIN	-3.179	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21971	DISHRVRSSDLFLHRPCISY			20	SLAY-screened peptide P321			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACATCAGTCACCGTGTGCGTTCTTCCGATTTGTTCCTTCATCGTCCTTGCATTTCTTATTAA	DISHRVRSSDLFLHRPCISY*	-3.172	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21972	SLHYGPWHDIFNTPMSHYLW			20	SLAY-screened peptide P322			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCTGCACTACGGTCCCTGGCATGATATTTTCAATACGCCCATGTCTCACTATCTTTGGTAA	SLHYGPWHDIFNTPMSHYLW*	-3.169	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21973	PCLYDSNCYCFNYCHRPNGE			20	SLAY-screened peptide P323			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGTCTCTATGATTCTAATTGCTATTGTTTCAATTACTGTCACAGGCCTAATGGCGAGTAA	PCLYDSNCYCFNYCHRPNGE*	-3.166	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21974	IATPCNLLDDVFDYTLATDS			20	SLAY-screened peptide P324			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGCGACGCCCTGTAATTTGTTGGATGATGTTTTTGATTATACGCTGGCGACTGACTCTTAA	IATPCNLLDDVFDYTLATDS*	-3.165	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21975	TSYLRYTPHTTLTIFIFVCPN			21	SLAY-screened peptide P325			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAGCTACCTGCGTTATACTCCGCACACCACTCTCACTATTTTCATTTTTGTGTGTCCTAAC	TSYLRYTPHTTLTIFIFVCPN	-3.155	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21976	CAASYIQDPASYACFNLKSA			20	SLAY-screened peptide P326			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCTGCTAGTTACATTCAGGATCCGGCTTCGTACGCCTGCTTTAACCTTAAGAGCGCTTAA	CAASYIQDPASYACFNLKSA*	-3.154	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21977	NSLA			4	SLAY-screened peptide P327			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATAGCTTGGCTTAGGAGCCTACGTTCTACGATGGTATTTATTATATTCCTAAAACTAGTAAC	NSLA*EPTFYDGIYYIPKTSN	-3.152	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21978	NYTRTHIQILAVPVITF			17	SLAY-screened peptide P328			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTATACGCGTACTCACATTCAGATCCTGGCTGTTCCGGTGATTACTTTTTAGTATTATTAA	NYTRTHIQILAVPVITF*YY*	-3.152	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21979	LPETEALPYRCNIWITLNKE			20	SLAY-screened peptide P329			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCTGAGACCGAGGCTTTGCCTTATCGCTGTAATATCTGGATTACTCTTAATAAGGAGTAA	LPETEALPYRCNIWITLNKE*	-3.148	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21980	LNTSSRINCFYIDPPDHLFS			20	SLAY-screened peptide P330			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAATACTAGTAGCCGCATTAATTGTTTTTACATTGACCCCCCTGACCATCTGTTTTCTTAA	LNTSSRINCFYIDPPDHLFS*	-3.147	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21981	RTNLLVMFSFLACMSIPMRI			20	SLAY-screened peptide P331			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACTAATTTGCTGGTTATGTTCTCCTTTTTGGCCTGTATGTCTATCCCCATGCGCATTTAA	RTNLLVMFSFLACMSIPMRI*	-3.144	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21982	EHAS			4	SLAY-screened peptide P332			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCACGCGAGCTAGACGTTCGATAATTCTCTTATCTATCCTCATCGTTGCATTTTTGATTAA	EHAS*TFDNSLIYPHRCIFD*	-3.144	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21983	YFPWKGIDY			9	SLAY-screened peptide P333			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTTCCCTTGGAAGGGTATTGATTATTAGACTGACGTCGACATGGCGTATTAGATTGTTTAA	YFPWKGIDY*TDVDMAY*IV*	-3.135	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21984	CNQSPFIYIACWGNGVIVHL			20	SLAY-screened peptide P334			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAACCAGTCTCCGTTCATTTACATCGCTTGTTGGGGTAATGGTGTTATTGTTCATCTTTAA	CNQSPFIYIACWGNGVIVHL*	-3.134	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21985	WIPPPQASDTTDGVASSKYD			20	SLAY-screened peptide P335			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGATTCCGCCTCCGCAGGCTAGTGATACTACTGATGGCGTTGCTAGTTCTAAGTACGATTAA	WIPPPQASDTTDGVASSKYD*	-3.133	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21986	HLDLHLNKSLHITLWYV			17	SLAY-screened peptide P336			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTTGGATTTGCACCTCAATAAGAGTCTCCATATTACTCTCTGGTACGTCTAGGGCTTAACT	HLDLHLNKSLHITLWYV*GLT	-3.133	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21987	VHTLQTYKDAALDTLYRVLFN			21	SLAY-screened peptide P337			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCATACGCTGCAGACGTACAAGGACGCCGCTCTTGATACTCTTTACAGGGTCCTGTTTAAC	VHTLQTYKDAALDTLYRVLFN	-3.132	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21988	AQAPDSRYDNTFIGHIDVMK			20	SLAY-screened peptide P338			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCAGGCGCCTGATAGTCGCTATGATAATACTTTTATCGGTCACATTGACGTTATGAAGTAA	AQAPDSRYDNTFIGHIDVMK*	-3.127	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21989	RKSYSLHICANDYNDKNLGPN			21	SLAY-screened peptide P339			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGAAGAGCTACTCTCTTCACATTTGCGCGAATGATTATAACGATAAGAATCTTGGACCTAAC	RKSYSLHICANDYNDKNLGPN	-3.124	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21990	NKCRPISKADVL			12	SLAY-screened peptide P340			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATAAGTGCCGCCCCATCTCGAAGGCTGACGTGTTGTAGATGGTTGACTCTAACAGCACTTAA	NKCRPISKADVL*MVDSNST*	-3.124	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21991	AHPRPVSAP			9	SLAY-screened peptide P341			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCATCCCCGTCCGGTGTCCGCTCCTTAGCACCATCACCCTTAGAACACGACCGTGCACTAA	AHPRPVSAP*HHHP*NTTVH*	-3.12	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21992	SSDYSDPLSWARSTCDNRNP			20	SLAY-screened peptide P342			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTCGGATTACAGTGATCCTCTTTCTTGGGCGCGGTCTACTTGCGACAATAGGAACCCTTAA	SSDYSDPLSWARSTCDNRNP*	-3.117	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21993	AFTNMLITAFCNPIYAMTVDL			21	SLAY-screened peptide P343			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTTCACGAACATGCTTATTACGGCTTTTTGCAACCCTATCTATGCTATGACCGTCGACCTG	AFTNMLITAFCNPIYAMTVDL	-3.114	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21994	VCDYHYNIHCLRRR			14	SLAY-screened peptide P344			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTGTGATTACCATTATAACATCCATTGTCTTCGCCGTCGTTAGTAGCCTCATAATAATTAA	VCDYHYNIHCLRRR**PHNN*	-3.106	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21995	IIDSGTQPGAFYLVMFRIVQ			20	SLAY-screened peptide P345			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTATTGATAGTGGTACTCAGCCTGGTGCTTTTTACCTGGTTATGTTCCGTATTGTTCAGTAA	IIDSGTQPGAFYLVMFRIVQ*	-3.102	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21996	GTSSPRKPIHNYRKENITND			20	SLAY-screened peptide P346			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTACTAGCTCTCCTCGTAAGCCCATTCATAACTACAGGAAGGAGAATATCACGAACGACTAA	GTSSPRKPIHNYRKENITND*	-3.101	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21997	VPFLPGIWVLPRPVRIASFAN			21	SLAY-screened peptide P347			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCCATTTTTGCCCGGTATATGGGTACTACCAAGACCTGTGCGCATCGCGTCGTTTGCTAAC	VPFLPGIWVLPRPVRIASFAN	-3.101	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21998	SGEDR			5	SLAY-screened peptide P348			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGGGGAGGACCGGTAGCCTCTCTCTGTCCCTATTGTTCTTGACCCTGATCAGGCTTCGTAA	SGEDR*PLSVPIVLDPDQAS*	-3.1	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP21999	MPRVHPTVDRNALYLIPVIN			20	SLAY-screened peptide P349			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCCTCGTGTCCACCCTACGGTGGATCGTAACGCTCTTTATCTCATTCCCGTTATTAACTAA	MPRVHPTVDRNALYLIPVIN*	-3.091	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22000	APRIDDIR			8	SLAY-screened peptide P350			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCCGCGCATTGATGACATTAGGTAGGGTCGCTCTAACAATATTGGGGCGCTGTTTTCGTAA	APRIDDIR*GRSNNIGALFS*	-3.09	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22001	AQSDWNTSVGSFHYSCAILY			20	SLAY-screened peptide P351			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCAGAGTGATTGGAACACTTCGGTCGGTAGTTTCCATTATAGTTGTGCTATCTTGTACTAA	AQSDWNTSVGSFHYSCAILY*	-3.086	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22002	LPFRWGGSVRYPMRRCTTLV			20	SLAY-screened peptide P352			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCTTTTCGTTGGGGTGGGTCTGTGCGTTACCCTATGCGCCGTTGTACTACTCTCGTCTAA	LPFRWGGSVRYPMRRCTTLV*	-3.08	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22003	RAHSSHRYNHVVYSISSYIY			20	SLAY-screened peptide P353			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCTCATAGCTCGCATCGTTATAACCATGTTGTCTACTCTATTTCTAGTTATATTTATTAA	RAHSSHRYNHVVYSISSYIY*	-3.072	4.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22004	AHRSGNFFPIYPSSLPMAFY			20	SLAY-screened peptide P354			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCATCGTTCGGGGAATTTTTTTCCCATTTACCCTAGTTCTCTTCCTATGGCTTTTTACTAA	AHRSGNFFPIYPSSLPMAFY*	-3.071	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22005	IVGDLLNKGFNSGDSFT			17	SLAY-screened peptide P355			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGTTGGCGACCTTCTTAATAAGGGGTTTAATTCGGGCGATAGCTTCACTTAGGCTAGTTAC	IVGDLLNKGFNSGDSFT*ASY	-3.066	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22006	PVNEDIQ			7	SLAY-screened peptide P356			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGTCAACGAGGACATCCAGTAGACCCTTACGACAACCATTACCACATGAGTCTTAGCTAAC	PVNEDIQ*TLTTTITT*VLAN	-3.065	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22007	RENRPSHWFVTQLCYLLCRH			20	SLAY-screened peptide P357			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGAGAATAGGCCCAGCCATTGGTTTGTCACTCAGCTTTGCTATTTGCTTTGCCGCCACTAA	RENRPSHWFVTQLCYLLCRH*	-3.065	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22008	PATTNGSR			8	SLAY-screened peptide P358			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCGACTACTAATGGCTCTCGGTAGACGTGCGCTGACATTTGCAATTCCCTGGATTCGTAA	PATTNGSR*TCADICNSLDS*	-3.065	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22009	STLLKSYHIFAYSMLPFWYH			20	SLAY-screened peptide P359			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTACTTTGCTTAAGTCCTATCATATCTTTGCGTACAGTATGTTGCCTTTTTGGTACCATTAA	STLLKSYHIFAYSMLPFWYH*	-3.064	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22010	GSKGSCTLYFNLIGFWTPTD			20	SLAY-screened peptide P360			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTCGAAGGGCTCTTGCACGCTGTACTTTAATCTTATCGGCTTTTGGACTCCGACGGACTAA	GSKGSCTLYFNLIGFWTPTD*	-3.063	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22011	RCHLHCYTTLNDPPHHRVS			19	SLAY-screened peptide P361			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTGTCACCTTCACTGTTACACTACCTTGAACGACCCTCCCCACCATCGTGTCAGCTAGTAA	RCHLHCYTTLNDPPHHRVS**	-3.061	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22012	LPEISHTRR			9	SLAY-screened peptide P362			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCCGAGATCAGCCACACTCGTCGTTAGATCCCGAAGGAGCGCTGTCACCGTTACAATTAA	LPEISHTRR*IPKERCHRYN*	-3.059	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22013	HTIVLLTLVTLRRRLYSFLK			20	SLAY-screened peptide P363			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACCATCGTCTTGCTCACCCTCGTCACGCTCCGGCGCCGGCTCTATAGCTTCCTCAAGTAA	HTIVLLTLVTLRRRLYSFLK*	-3.058	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22014	AHLSNSIDPFHAGSVHFTPD			20	SLAY-screened peptide P364			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCATCTTAGCAACAGTATTGATCCTTTCCATGCTGGCAGCGTCCACTTCACCCCTGACTAA	AHLSNSIDPFHAGSVHFTPD*	-3.056	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22015	LKWYCHFNSTQNLRAQTNIG			20	SLAY-screened peptide P365			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAAGTGGTACTGTCATTTTAATAGTACCCAGAATCTGCGCGCCCAGACGAACATCGGCTAA	LKWYCHFNSTQNLRAQTNIG*	-3.055	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22016	CGGLLAWTGPLSECIQFWLL			20	SLAY-screened peptide P366			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGGTGGCTTGCTGGCGTGGACCGGCCCGCTGAGCGAGTGCATTCAGTTCTGGCTTCTTTAA	CGGLLAWTGPLSECIQFWLL*	-3.052	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22017	PP			2	SLAY-screened peptide P367			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCGTAGCCGATCACGACGCATACGTAGTCGCCGAGTAATCTGCTGGCTGTGATGATGTAA	PP*PITTHT*SPSNLLAVMM*	-3.052	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22018	LRGLLSFSSYQMVMDGDTITN			21	SLAY-screened peptide P368			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCGCGGCCTTCTTAGCTTCTCTTCCTATCAGATGGTTATGGACGGTGATACTATTACTAAC	LRGLLSFSSYQMVMDGDTITN	-3.052	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22019	TTLFNVSLHMVNTSGSTGTN			20	SLAY-screened peptide P369			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACCCTGTTTAACGTTTCCTTGCATATGGTTAATACTAGCGGTTCTACTGGTACTAACTAA	TTLFNVSLHMVNTSGSTGTN*	-3.05	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22020	AGNWLMAGLSLAPARPSPNG			20	SLAY-screened peptide P370			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGGCAACTGGCTTATGGCCGGTCTCAGCCTTGCTCCTGCGCGTCCTAGCCCTAATGGCTAA	AGNWLMAGLSLAPARPSPNG*	-3.048	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22021	LSLHCDIGFNANNTLSTDYI			20	SLAY-screened peptide P371			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCCTTGCATTGTGATATTGGTTTTAACGCCAATAATACGCTGTCCACCGATTACATTTAA	LSLHCDIGFNANNTLSTDYI*	-3.046	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22022	FLVAVRINFNLNIRFYIDLS			20	SLAY-screened peptide P372			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCTTGTCGCCGTGCGCATTAATTTTAACCTCAATATCCGCTTTTATATCGACCTCTCTTAA	FLVAVRINFNLNIRFYIDLS*	-3.042	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22023	FIPHHNHSLAYETIVSGRDP			20	SLAY-screened peptide P373			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCATTCCTCATCATAATCATAGCCTCGCTTACGAGACGATTGTTAGTGGGCGTGACCCGTAA	FIPHHNHSLAYETIVSGRDP*	-3.038	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22024	HHRSRKMYNWNHNEANRHYQ			20	SLAY-screened peptide P374			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCATAGGAGTCGCAAGATGTATAACTGGAACCATAACGAGGCTAACCGTCACTATCAGTAA	HHRSRKMYNWNHNEANRHYQ*	-3.036	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22025	IICLELANDDLCCKCHSSDD			20	SLAY-screened peptide P375			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTATTTGCCTGGAGCTGGCTAATGATGATCTCTGTTGCAAGTGTCATTCTTCCGACGACTAA	IICLELANDDLCCKCHSSDD*	-3.035	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22026	LAESALLRGNNSCNLTFIRN			20	SLAY-screened peptide P376			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGCCGAGAGCGCTCTGTTGCGTGGCAATAATAGTTGTAATTTGACTTTTATCAGGAACTAA	LAESALLRGNNSCNLTFIRN*	-3.035	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22027	YSHVCKTNTSHCYTFHYNGF			20	SLAY-screened peptide P377			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTCTCACGTTTGTAAGACTAATACTTCCCATTGTTATACTTTTCATTACAATGGCTTCTAA	YSHVCKTNTSHCYTFHYNGF*	-3.033	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22028	SQP			3	SLAY-screened peptide P378			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCAGCCTTAGCTGATCCCCATTACTAACTAGCATTCTAGTTCGTTCATGCCCGCCGACTAA	SQP*LIPITN*HSSSFMPAD*	-3.022	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22029	KHRF			4	SLAY-screened peptide P379			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCATCGCTTTTAGCATGCTTATGCGCCGTGGTACGCTTTTAACTTTGTCTGTCGCTATTAA	KHRF*HAYAPWYAFNFVCRY*	-3.018	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22030	LCALTQASTLSNNHTTHLAT			20	SLAY-screened peptide P380			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTGCGCCCTCACTCAGGCCTCTACTCTTAGTAACAACCATACTACGCATTTGGCTACGTAA	LCALTQASTLSNNHTTHLAT*	-3.012	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22031	WFAPCKSAAIHAF			13	SLAY-screened peptide P381			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTTTGCCCCCTGCAAGAGTGCCGCCATCCATGCTTTTTAGCTTCTCAACCATGAGGCTTAA	WFAPCKSAAIHAF*LLNHEA*	-3.01	0.000101	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22032	PRTLLTTALRILYTKGLLGD			20	SLAY-screened peptide P382			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGCACTTTGCTTACTACTGCGCTTCGCATTCTTTATACTAAGGGGCTTCTTGGCGACTAA	PRTLLTTALRILYTKGLLGD*	-3.008	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22033	VFSSAYRADAKGTSSFNSTQ			20	SLAY-screened peptide P383			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTTCTCGTCGGCGTATCGTGCTGACGCTAAGGGCACGTCGAGTTTCAACTCGACGCAGTAA	VFSSAYRADAKGTSSFNSTQ*	-3.008	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22034	PNISTGPSFILPLLLGCIAFN			21	SLAY-screened peptide P384			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAACATTTCTACCGGTCCAAGTTTTATACTACCCCTTCTCCTGGGTTGCATCGCATTTAAC	PNISTGPSFILPLLLGCIAFN	-3.008	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22035	YSTSSCSCDYQSSSYR			16	SLAY-screened peptide P385			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAGTACTTCGAGTTGTTCTTGTGATTACCAATCGTCATCTTATCGGTAACTGAGTAAGTCG	YSTSSCSCDYQSSSYR*LSKS	-3.006	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22036	HAALGCQHYPNMRTELDHTK			20	SLAY-screened peptide P386			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCGGCCCTCGGTTGTCAGCATTATCCGAACATGCGGACTGAGCTCGATCACACTAAGTAA	HAALGCQHYPNMRTELDHTK*	-3.005	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22037	ASSVSSFVLYSARSFNNSSH			20	SLAY-screened peptide P387			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTAGTTCTGTGTCCAGTTTTGTGCTCTATTCTGCTCGTTCTTTCAATAATAGTTCTCATTAA	ASSVSSFVLYSARSFNNSSH*	-3.004	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22038	VALDTTFSHRSPP			13	SLAY-screened peptide P388			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGCGCTTGACACTACTTTTAGTCATCGGAGTCCCCCTTAGACCTTTTATATTAAGAATTAA	VALDTTFSHRSPP*TFYIKN*	-3.002	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22039	LVYTDLYGFFSDLRPRNQDD			20	SLAY-screened peptide P389			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGGTGTACACTGACCTTTACGGTTTTTTTAGCGACCTTCGCCCTAGGAACCAGGACGATTAA	LVYTDLYGFFSDLRPRNQDD*	-3.001	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22040	KNVNHSVIVNPNFDPNTVTR			20	SLAY-screened peptide P390			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGAATGTTAATCATAGTGTCATCGTCAACCCGAATTTCGATCCGAATACTGTTACGAGGTAA	KNVNHSVIVNPNFDPNTVTR*	-2.997	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22041	STTLYLPGLNRIRTNDFMIT			20	SLAY-screened peptide P391			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGACCACTCTGTATCTGCCTGGTCTTAACCGGATCAGGACGAACGATTTTATGATCACCTAA	STTLYLPGLNRIRTNDFMIT*	-2.995	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22042	STLINVFDR			9	SLAY-screened peptide P392			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTACCCTCATTAACGTTTTCGACAGGTAGGTGTATAAGTCGTGGCCGATTTACTGGTCCTAA	STLINVFDR*VYKSWPIYWS*	-2.993	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22043	LVTDAMWHGLHVSRCHSHYY			20	SLAY-screened peptide P393			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGTTACTGATGCCATGTGGCATGGGCTTCATGTTTCGCGTTGTCATAGTCACTACTACTAA	LVTDAMWHGLHVSRCHSHYY*	-2.988	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22044	GPIHDVLRMIRSSGTTHFYS			20	SLAY-screened peptide P394			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCCTATCCATGATGTGCTTAGGATGATTCGTAGCTCCGGGACGACCCACTTTTATAGCTAA	GPIHDVLRMIRSSGTTHFYS*	-2.986	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22045	LPTNSIRRDGLSADHHRYIRN			21	SLAY-screened peptide P395			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCCACTAATAGTATTCGTCGTGACGGCCTCAGCGCTGATCATCATCGCTACATCCGTAAC	LPTNSIRRDGLSADHHRYIRN	-2.985	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22046	LMRSVFLNMCIPSDYMDPSVP			21	SLAY-screened peptide P396			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCATGCGTAGTGTCTTTCTTAATATGTGCATCCCTTCTGACTATATGGACCCCAGTGTGCCC	LMRSVFLNMCIPSDYMDPSVP	-2.98	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22047	NKNPIRLSFYPHNYYLYSSV			20	SLAY-screened peptide P397			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATAAGAATCCCATCAGGCTTAGTTTCTATCCTCATAATTATTATTTGTATTCCAGTGTTTAA	NKNPIRLSFYPHNYYLYSSV*	-2.977	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22048	TSLCFVIILNLKSDMAG			17	SLAY-screened peptide P398			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTCTCTGTGCTTCGTCATTATTCTTAATCTCAAGAGTGATATGGCGGGCTAGTGTTCGTAA	TSLCFVIILNLKSDMAG*CS*	-2.975	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22049	HNNPPAPNGPHSTFIADNAS			20	SLAY-screened peptide P399			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAACAATCCGCCGGCCCCTAACGGGCCTCATAGTACGTTTATCGCTGATAATGCTAGTTAA	HNNPPAPNGPHSTFIADNAS*	-2.97	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22050	WTYFHSNPHEYQLNLLIANM			20	SLAY-screened peptide P400			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGACCTACTTTCATAGTAACCCGCATGAGTATCAGCTTAACCTTCTTATTGCCAATATGTAA	WTYFHSNPHEYQLNLLIANM*	-2.966	6.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22051	LIFTLQNRLQPVAMHKPCYS			20	SLAY-screened peptide P401			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATCTTTACCTTGCAGAACCGCCTCCAGCCGGTGGCCATGCATAAGCCCTGTTACTCCTAA	LIFTLQNRLQPVAMHKPCYS*	-2.962	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22052	PDHNNHT			7	SLAY-screened peptide P402			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGATCACAATAATCATACGTAGTTCATGTTTAGTACCACTACGTTGTACCATGAGCCGTAA	PDHNNHT*FMFSTTTLYHEP*	-2.96	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22053	YHNHSHD			7	SLAY-screened peptide P403			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCATAATCACTCTCATGATTAGGATGCCGCCCTGTACCTTGGTTCCATGTAGATGATCTAA	YHNHSHD*DAALYLGSM*MI*	-2.958	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22054	ALTQHRLGLRNIPQNLYIMV			20	SLAY-screened peptide P404			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCTCACTCAGCATCGTCTCGGGCTTAGGAATATCCCTCAGAATCTCTATATTATGGTCTAA	ALTQHRLGLRNIPQNLYIMV*	-2.955	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22055	ASFTHPPIMAPPIYASSEVE			20	SLAY-screened peptide P405			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTCTTTTACTCATCCTCCTATCATGGCCCCGCCTATCTACGCTTCTAGTGAGGTTGAGTAA	ASFTHPPIMAPPIYASSEVE*	-2.955	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22056	HTLQQLRCPHCSLSNNSMVY			20	SLAY-screened peptide P406			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACTCTTCAGCAGCTTCGTTGTCCGCATTGCAGCCTTAGCAATAATTCTATGGTGTACTAA	HTLQQLRCPHCSLSNNSMVY*	-2.951	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22057	TISRGNSPPSANTALLMNYI			20	SLAY-screened peptide P407			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGATTTCTCGCGGTAATTCGCCTCCTTCTGCTAATACTGCGCTTCTCATGAACTACATTTAA	TISRGNSPPSANTALLMNYI*	-2.948	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22058	TSPMQSLRLLTSISLKNRVM			20	SLAY-screened peptide P408			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCTCCTATGCAGTCCCTGCGTCTCCTCACGAGCATCTCGTTGAAGAACAGGGTTATGTAA	TSPMQSLRLLTSISLKNRVM*	-2.945	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22059	IAVGPVGRIRFPRLTFRFTL			20	SLAY-screened peptide P409			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGCCGTGGGCCCTGTGGGCCGCATCCGTTTTCCTAGGCTCACTTTTCGCTTTACCCTGTAA	IAVGPVGRIRFPRLTFRFTL*	-2.944	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22060	SGRQTGNHNCYMSLQLLTIC			20	SLAY-screened peptide P410			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGGCCGTCAGACGGGGAACCATAATTGTTATATGTCTCTTCAGCTCCTGACCATCTGCTAA	SGRQTGNHNCYMSLQLLTIC*	-2.939	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22061	QSAAGMPSVD			10	SLAY-screened peptide P411			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTCCGCCGCTGGTATGCCTAGCGTTGATTAGGTGTTGTATCCTCATGTGCGTTCCGTTTAA	QSAAGMPSVD*VLYPHVRSV*	-2.937	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22062	PRIFCILLPRPCSGHVFYAS			20	SLAY-screened peptide P412			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGGATTTTCTGTATTTTGCTTCCTCGGCCCTGTTCTGGCCACGTGTTCTATGCTAGTTAA	PRIFCILLPRPCSGHVFYAS*	-2.927	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22063	VSFFTVPLWHCLPSDLLALN			20	SLAY-screened peptide P413			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTCTTTTTTTACCGTGCCGCTGTGGCATTGTCTGCCGAGTGATCTTTTGGCGCTGAATTAA	VSFFTVPLWHCLPSDLLALN*	-2.919	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22064	ALRTMY			6	SLAY-screened peptide P414			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCTTCGCACTATGTATTAGTTTTACAATTATATCTTACCTGTAAGAACAATAGGAATTAAC	ALRTMY*FYNYILPVRTIGIN	-2.911	4.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22065	RTLPFCAPGIVLTLI			15	SLAY-screened peptide P415			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGAACGTTACCATTTTGCGCACCGGGAATAGTTCTTACACTTATATGACCATCTTGTTCTAAC	RTLPFCAPGIVLTLI*PSCSN	-2.911	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22066	CVVDPLY			7	SLAY-screened peptide P416			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGTCGTGGACCCTCTCTATTAGTATTGGGCCATCCTCTCCTTTTGCCGTAGGTACCCTTAA	CVVDPLY*YWAILSFCRRYP*	-2.903	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22067	RPIIPYSAHSYLCVTTYNPT			20	SLAY-screened peptide P417			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCCATTATTCCTTATAGCGCTCACTCTTATCTGTGTGTCACCACCTACAATCCTACGTAA	RPIIPYSAHSYLCVTTYNPT*	-2.902	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22068	LRVPIVPISS			10	SLAY-screened peptide P418			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCGTGTGCCCATTGTTCCTATTTCGAGTTAGTGTCAGAACGTCTTCAATGGCGACTCTTAA	LRVPIVPISS*CQNVFNGDS*	-2.901	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22069	YRHTAN			6	SLAY-screened peptide P419			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAGGCACACTGCTAACTAGCTCCGTGAGTATCTTGGGTAGGCGACGCTGGAGAGTGCTTAA	YRHTAN*LREYLG*ATLESA*	-2.899	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22070	RAN			3	SLAY-screened peptide P420			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGCGAATTAGCACCTCCCTCATGAGACTCATGATACGGTTGAGTCGTCCATGAATAGCTAA	RAN*HLPHETHDTVESSMNS*	-2.892	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22071	MLDYFLGHSYLSLVDEDPNR			20	SLAY-screened peptide P421			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTTGGACTACTTTCTCGGTCACAGTTATCTCAGCCTCGTTGATGAGGATCCTAACAGGTAA	MLDYFLGHSYLSLVDEDPNR*	-2.888	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22072	APLFFGLCIVCTTDGRRKSF			20	SLAY-screened peptide P422			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCCTTGTTTTTTGGCCTTTGTATCGTCTGTACTACGGATGGTCGCCGGAAGAGCTTTTAA	APLFFGLCIVCTTDGRRKSF*	-2.885	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22073	TCVDITATICAVTWIVIDFA			20	SLAY-screened peptide P423			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTGTGTTGATATTACTGCGACTATTTGTGCTGTTACGTGGATTGTGATTGATTTTGCCTAA	TCVDITATICAVTWIVIDFA*	-2.884	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22074	NLL			3	SLAY-screened peptide P424			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCTGCTGTAGCTTTATTAGAATTCGGTTCAGAGCCTTGTTACGGGTTGCCATTGGTTTTAA	NLL*LY*NSVQSLVTGCHWF*	-2.884	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22075	DWTYTYVSRPIASLADLHAI			20	SLAY-screened peptide P425			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTGGACCTACACTTATGTGTCCCGGCCTATTGCTTCTCTTGCTGACCTCCATGCGATCTAA	DWTYTYVSRPIASLADLHAI*	-2.879	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22076	MAGSVAYTSSFSNPCTVNHY			20	SLAY-screened peptide P426			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGCCGGTTCCGTTGCCTATACTTCGTCGTTCTCTAACCCTTGTACTGTCAATCATTATTAA	MAGSVAYTSSFSNPCTVNHY*	-2.876	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22077	LIFIVLSHSTPHARGPPGRA			20	SLAY-screened peptide P427			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGATTTTTATCGTGCTTTCCCATAGTACGCCGCACGCGAGGGGCCCCCCGGGGCGTGCCTAA	LIFIVLSHSTPHARGPPGRA*	-2.876	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22078	LLFAFPVPGNVPEVLAENTP			20	SLAY-screened peptide P428			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTTTTTGCTTTCCCTGTCCCTGGTAATGTCCCTGAGGTTCTTGCTGAGAACACTCCCTAA	LLFAFPVPGNVPEVLAENTP*	-2.867	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22079	DIVSLSRRIPFERTFDPK			18	SLAY-screened peptide P429			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATATTGTGTCCTTGTCGCGTAGGATCCCCTTTGAGCGCACGTTTGACCCCAAGTAGAAGTAA	DIVSLSRRIPFERTFDPK*K*	-2.866	4.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22080	NFHDETIKLLSPNLYALAIS			20	SLAY-screened peptide P430			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTTCCACGATGAGACTATTAAGTTGCTTAGTCCTAATCTCTACGCGCTGGCTATTAGTTAA	NFHDETIKLLSPNLYALAIS*	-2.864	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22081	PAVGNYSYVFINSLTAGFLV			20	SLAY-screened peptide P431			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGCCGTGGGCAACTATTCTTACGTGTTCATTAACAGCCTTACTGCGGGTTTTCTGGTGTAA	PAVGNYSYVFINSLTAGFLV*	-2.862	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22082	SRITANNSHIITRETKLCYW			20	SLAY-screened peptide P432			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCGGATTACCGCTAATAATAGCCATATTATTACCCGTGAGACCAAGTTGTGCTACTGGTAA	SRITANNSHIITRETKLCYW*	-2.862	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22083	HGHASDYIDPHGAQC			15	SLAY-screened peptide P433			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGGGCATGCGTCCGATTACATTGACCCGCATGGGGCCCAGTGTTAGACCAGGTGCCACTAA	HGHASDYIDPHGAQC*TRCH*	-2.856	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22084	CNSYPVYDHHSHTAYDQFQ			19	SLAY-screened peptide P434			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAATAGTTACCCTGTGTATGATCATCACAGTCACACGGCTTATGATCAGTTTCAGTAGTAA	CNSYPVYDHHSHTAYDQFQ**	-2.855	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22085	YGGYSIRFSHYYIYMSSPHL			20	SLAY-screened peptide P435			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGGCGGGTATTCTATCAGGTTCTCCCATTATTATATTTACATGTCCAGTCCGCATTTGTAA	YGGYSIRFSHYYIYMSSPHL*	-2.854	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22086	CID			3	SLAY-screened peptide P436			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCATTGATTAGTCTAGCGCTCTGCTGCGGCCGAGTATGTAGATGCAGGTTTCTCCCGTTTAA	CID*SSALLRPSM*MQVSPV*	-2.852	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22087	PSAAVGLIPFLMARANYYLT			20	SLAY-screened peptide P437			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGTGCGGCCGTCGGCCTGATCCCCTTTCTTATGGCTAGGGCGAACTACTATCTGACGTAA	PSAAVGLIPFLMARANYYLT*	-2.851	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22088	TLETRFYLFYTLDTMMSKHN			20	SLAY-screened peptide P438			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTGGAGACTCGCTTTTACCTTTTTTATACTCTTGACACGATGATGTCGAAGCACAACTAA	TLETRFYLFYTLDTMMSKHN*	-2.851	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22089	CRNCVYHHYNISPNASPASD			20	SLAY-screened peptide P439			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAGGAACTGCGTTTATCACCATTACAATATTAGCCCTAATGCCTCGCCTGCTTCCGATTAA	CRNCVYHHYNISPNASPASD*	-2.849	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22090	DHPSTCHHGVGPCLFLNYNI			20	SLAY-screened peptide P440			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCACCCTAGTACCTGTCATCATGGCGTTGGCCCGTGCCTCTTTCTCAATTACAATATCTAA	DHPSTCHHGVGPCLFLNYNI*	-2.846	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22091	IDHCIVGVRNSLARVLANGF			20	SLAY-screened peptide P441			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGATCATTGTATTGTGGGCGTTCGCAATAGCTTGGCGAGGGTTCTCGCGAACGGGTTTTAA	IDHCIVGVRNSLARVLANGF*	-2.842	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22092	SYNGPSDSPHTHSRHCSFQR			20	SLAY-screened peptide P442			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTATAATGGTCCTTCCGATTCTCCCCATACTCATTCTCGGCATTGTTCGTTCCAGCGCTAA	SYNGPSDSPHTHSRHCSFQR*	-2.84	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22093	YIGVPGLASRYVLSVLLHGV			20	SLAY-screened peptide P443			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATATCGGTGTTCCGGGTCTGGCCTCCCGTTACGTTCTTTCTGTCTTGCTTCACGGTGTCTAA	YIGVPGLASRYVLSVLLHGV*	-2.839	2.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22094	VEHPISLRFFFGVRSVCVIN			20	SLAY-screened peptide P444			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGAGCACCCTATTAGCCTGCGCTTTTTTTTCGGTGTGCGCAGCGTTTGCGTTATTAATTAA	VEHPISLRFFFGVRSVCVIN*	-2.839	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22095	WLPPHRDPRL			10	SLAY-screened peptide P445			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCTGCCCCCGCATCGCGACCCCAGGCTTTAGTCCTCCCTGCCTGCTCAGCCGGACGTCTAA	WLPPHRDPRL*SSLPAQPDV*	-2.839	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22096	EILLLIRIGILILWIMISLGN			21	SLAY-screened peptide P446			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGATACTTCTTCTCATACGCATAGGCATACTAATTTTGTGGATAATGATCTCACTGGGTAAC	EILLLIRIGILILWIMISLGN	-2.836	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22097	YFTHPHIYAVSPTVTQFFIA			20	SLAY-screened peptide P447			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTTCACTCATCCTCATATTTATGCTGTGTCTCCCACTGTCACCCAGTTTTTTATTGCCTAA	YFTHPHIYAVSPTVTQFFIA*	-2.835	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22098	FTQAREAPCTPDMSSDH			17	SLAY-screened peptide P448			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTACCCAGGCGCGCGAGGCGCCCTGCACCCCCGACATGTCTAGTGATCATTAGTTTCGCTAA	FTQAREAPCTPDMSSDH*FR*	-2.834	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22099	CPTVLDYHSRDSTTTFSLEP			20	SLAY-screened peptide P449			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCGACTGTGCTTGACTACCATTCGCGCGATTCTACTACTACGTTTTCGCTCGAGCCCTAA	CPTVLDYHSRDSTTTFSLEP*	-2.832	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22100	FTPTCCVTRLHTSAQLRVRH			20	SLAY-screened peptide P450			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTACCCCGACTTGCTGCGTCACTCGCCTGCATACTAGCGCTCAGCTTCGCGTTAGGCATTAA	FTPTCCVTRLHTSAQLRVRH*	-2.831	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22101	RPNWNIRSCIQCEFQIQ			17	SLAY-screened peptide P451			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCGAATTGGAATATCAGGAGTTGCATCCAGTGCGAGTTTCAGATTCAGTAGTATATGTAA	RPNWNIRSCIQCEFQIQ*YM*	-2.831	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22102	TAPRVAAPHTLHCNRWWLTP			20	SLAY-screened peptide P452			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGCGCCCCGGGTCGCCGCCCCGCATACGCTTCACTGTAATCGTTGGTGGCTCACCCCTTAA	TAPRVAAPHTLHCNRWWLTP*	-2.829	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22103	DLYHSYHDCHHNTA			14	SLAY-screened peptide P453			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCTCTATCATAGTTATCACGATTGCCATCATAATACTGCTTAGTTTATGAACACCTATTAA	DLYHSYHDCHHNTA*FMNTY*	-2.828	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22104	TPRDVDADLGPVATPRTIFM			20	SLAY-screened peptide P454			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCCCCGCGATGTGGATGCTGATCTGGGTCCTGTCGCCACTCCCCGTACCATCTTTATGTAA	TPRDVDADLGPVATPRTIFM*	-2.826	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22105	LSSNDRPAKYKDSDCGHSYL			20	SLAY-screened peptide P455			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAGCAGCAACGATCGCCCCGCCAAGTACAAGGATAGTGACTGCGGTCATTCCTATTTGTAA	LSSNDRPAKYKDSDCGHSYL*	-2.826	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22106	TNGHDRKKDTFSCPFISNRH			20	SLAY-screened peptide P456			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAACGGGCACGACCGTAAGAAGGATACGTTTTCGTGTCCTTTTATCAGTAACCGTCATTAA	TNGHDRKKDTFSCPFISNRH*	-2.825	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22107	NSPFFQNNRYIHAAFDSDLT			20	SLAY-screened peptide P457			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACAGCCCGTTTTTTCAGAATAACCGCTACATTCACGCGGCTTTCGATAGCGACCTCACTTAA	NSPFFQNNRYIHAAFDSDLT*	-2.824	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22108	SSFRETYYYIPALYFVWGTR			20	SLAY-screened peptide P458			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTCTTTCCGGGAGACTTACTATTATATTCCCGCTCTGTACTTTGTTTGGGGTACGCGCTAA	SSFRETYYYIPALYFVWGTR*	-2.823	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22109	LYVSIILLVGRITFCMTILSN			21	SLAY-screened peptide P459			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTACGTTTCTATCATCTTGCTTGTCGGTCGAATAACCTTCTGCATGACCATACTGTCTAAC	LYVSIILLVGRITFCMTILSN	-2.819	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22110	FHQKVSGILNRDSINHFDSA			20	SLAY-screened peptide P460			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCATCAGAAGGTGTCCGGGATCCTTAACCGTGATTCTATTAATCATTTCGATTCTGCTTAA	FHQKVSGILNRDSINHFDSA*	-2.818	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22111	VLSNARSGTFATHGYLLVRY			20	SLAY-screened peptide P461			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCTTAGCAATGCTAGGTCTGGGACTTTTGCTACGCATGGGTACCTTCTTGTGCGCTATTAA	VLSNARSGTFATHGYLLVRY*	-2.818	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22112	AMSHLLHRQYPHIRSNDPDA			20	SLAY-screened peptide P462			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCATGTCGCATCTCCTTCATCGCCAGTATCCTCATATCCGGAGTAATGATCCGGATGCTTAA	AMSHLLHRQYPHIRSNDPDA*	-2.814	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22113	YGYCCESPGFQPFGRANGSE			20	SLAY-screened peptide P463			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGGTTACTGTTGCGAGTCCCCGGGGTTCCAGCCTTTCGGCCGCGCTAACGGCTCTGAGTAA	YGYCCESPGFQPFGRANGSE*	-2.812	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22114	DFVNRLRRFLCNRMHPNAAH			20	SLAY-screened peptide P464			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTTCGTCAATCGGCTGCGTCGGTTTTTGTGCAACCGGATGCACCCCAATGCCGCCCATTAA	DFVNRLRRFLCNRMHPNAAH*	-2.811	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22115	YLHRPLFSCDLMYVV			15	SLAY-screened peptide P465			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCTTCACCGGCCGCTGTTCTCGTGCGATCTTATGTATGTTGTTTAGCCTTCTCTGCACTAA	YLHRPLFSCDLMYVV*PSLH*	-2.798	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22116	YMHCSHPCPDLYR			13	SLAY-screened peptide P466			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATATGCATTGTTCTCATCCTTGTCCGGATCTTTACAGGTGACCCTCAATAATATTTGGTAAC	YMHCSHPCPDLYR*PSIIFGN	-2.798	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22117	TLFTSNQCPYYHHSSTCYRS			20	SLAY-screened peptide P467			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCTTTTTACCTCGAATCAGTGTCCGTATTACCATCACAGTAGTACCTGCTACAGGTCCTAA	TLFTSNQCPYYHHSSTCYRS*	-2.797	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22118	CPNISTNCRDTDIKKELSTRN			21	SLAY-screened peptide P468			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCTAATATTAGTACTAATTGTCGCGATACTGACATTAAGAAGGAACTTTCGACACGTAAC	CPNISTNCRDTDIKKELSTRN	-2.796	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22119	TQNYLSDT			8	SLAY-screened peptide P469			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCAGAATTATTTGTCCGATACGTAGAACCGCCCTATCGCGTTCGCTCGCGGTAATCTGTAA	TQNYLSDT*NRPIAFARGNL*	-2.795	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22120	ARHVFRTNIVLLDIDYSNMS			20	SLAY-screened peptide P470			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCGCCATGTTTTTCGGACTAATATTGTTCTTCTTGATATTGACTATAGCAATATGTCCTAA	ARHVFRTNIVLLDIDYSNMS*	-2.792	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22121	VVHLVGFTNNRHRDDL			16	SLAY-screened peptide P471			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGTCCACCTGGTTGGGTTTACTAATAACCGTCATCGGGATGATCTCTAGCACCGCTATTAA	VVHLVGFTNNRHRDDL*HRY*	-2.791	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22122	RDFSWGTPRYWNHMYYNNIL			20	SLAY-screened peptide P472			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGATTTTAGCTGGGGCACTCCGAGGTACTGGAATCATATGTACTATAATAATATCCTTTAA	RDFSWGTPRYWNHMYYNNIL*	-2.791	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22123	GNRVPATVCPIAISIPLMVD			20	SLAY-screened peptide P473			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTAACCGCGTTCCTGCGACGGTCTGCCCTATTGCTATTTCGATCCCCCTTATGGTCGACTAA	GNRVPATVCPIAISIPLMVD*	-2.788	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22124	CPSPKCAIVYQTIGPALPRA			20	SLAY-screened peptide P474			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCTAGTCCTAAGTGTGCTATTGTTTACCAGACTATCGGCCCTGCGCTCCCTCGGGCCTAA	CPSPKCAIVYQTIGPALPRA*	-2.785	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22125	KR			2	SLAY-screened peptide P475			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCGCTAGACTCGTACTGGTCGTTTCATCATCGATCACACTAAGCAGAAGGATAGGTACTAA	KR*TRTGRFIIDHTKQKDRY*	-2.785	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22126	ITNIVTMQGAHSGFHRDTRT			20	SLAY-screened peptide P476			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCACTAATATTGTTACTATGCAGGGTGCTCACAGTGGGTTCCACCGCGACACGAGGACGTAA	ITNIVTMQGAHSGFHRDTRT*	-2.783	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22127	GTTSNCDIYANIYTTDLYCG			20	SLAY-screened peptide P477			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCACTACTAGCAATTGTGACATCTACGCGAACATTTACACTACTGACCTTTACTGCGGTTAA	GTTSNCDIYANIYTTDLYCG*	-2.78	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22128	YQSPSHGYGFPLMNPCYILA			20	SLAY-screened peptide P478			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCAGTCTCCTTCGCATGGTTATGGCTTCCCTTTGATGAACCCTTGCTATATTCTGGCCTAA	YQSPSHGYGFPLMNPCYILA*	-2.775	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22129	STILSTTI			8	SLAY-screened peptide P479			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTACCATCTTGAGTACTACTATCTAGGCCTCTGCGATTGATTGGACGACGCTCTATCTTTAA	STILSTTI*ASAIDWTTLYL*	-2.77	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22130	DFLRCLTDLNKDITTLQSLD			20	SLAY-screened peptide P480			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTTCCTCCGTTGCCTCACTGATCTTAACAAGGATATTACTACGCTTCAGAGTCTCGACTAA	DFLRCLTDLNKDITTLQSLD*	-2.769	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22131	CSYLGFGKFFYL			12	SLAY-screened peptide P481			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTCTTATCTCGGCTTTGGCAAGTTTTTCTATCTCTAGAAGCCTTACCTCTTGCGTGAGTAA	CSYLGFGKFFYL*KPYLLRE*	-2.763	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22132	ASIHSSGKRPTFTAHRMLVE			20	SLAY-screened peptide P482			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTCCATTCACAGTTCGGGGAAGCGTCCGACTTTCACCGCTCACAGGATGTTGGTGGAGTAA	ASIHSSGKRPTFTAHRMLVE*	-2.76	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22133	RQPDWAVLGSVQCPSPNRPF			20	SLAY-screened peptide P483			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCAGCCGGATTGGGCGGTCCTCGGCTCTGTCCAGTGTCCGTCTCCTAATCGTCCTTTTTAA	RQPDWAVLGSVQCPSPNRPF*	-2.757	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22134	IPTPF			5	SLAY-screened peptide P484			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATACCGACTCCATTTTGAAGAATCTGTGTGACTTCTACAAGCGGACTCTCACCCACTATTAAC	IPTPF*RICVTSTSGLSPTIN	-2.754	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22135	LEPDFPWCGYNCGNRRRHHS			20	SLAY-screened peptide P485			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGAGCCGGACTTCCCCTGGTGTGGCTACAACTGTGGTAACCGTAGGCGGCATCATTCTTAA	LEPDFPWCGYNCGNRRRHHS*	-2.753	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22136	TAPITLIRGPPSGHGYSACH			20	SLAY-screened peptide P486			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGCCCCTATCACTCTCATCCGCGGCCCGCCGAGCGGGCATGGTTACAGTGCGTGTCATTAA	TAPITLIRGPPSGHGYSACH*	-2.752	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22137	SPIIKLYNEDVAHYDDLNI			19	SLAY-screened peptide P487			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCCGATTATTAAGCTCTACAATGAGGATGTTGCTCATTATGACGACCTTAACATCTAGTAA	SPIIKLYNEDVAHYDDLNI**	-2.751	3.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22138	PPLFSGSGCNNHADYRSTSS			20	SLAY-screened peptide P488			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTCTTTTTAGCGGTTCTGGTTGCAATAACCATGCTGACTATCGGTCCACTAGTTCCTAA	PPLFSGSGCNNHADYRSTSS*	-2.749	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22139	TDSMDFRSFDDAVGDIVYSA			20	SLAY-screened peptide P489			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGATAGTATGGACTTCCGTAGCTTTGACGACGCCGTCGGGGATATCGTTTACTCTGCGTAA	TDSMDFRSFDDAVGDIVYSA*	-2.748	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22140	PATATGRCIGPVPYSTSDNL			20	SLAY-screened peptide P490			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCGACTGCCACGGGGCGCTGTATCGGCCCCGTGCCTTATTCTACCTCCGACAATCTTTAA	PATATGRCIGPVPYSTSDNL*	-2.747	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22141	DIPNLRVYYYDRHFTLIYMK			20	SLAY-screened peptide P491			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATATCCCTAACTTGCGCGTCTACTATTACGACCGTCACTTTACGCTTATTTATATGAAGTAA	DIPNLRVYYYDRHFTLIYMK*	-2.745	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22142	RNNNNSHAIHCSNTRDLGAC			20	SLAY-screened peptide P492			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAATAACAATAACAGCCATGCTATCCATTGTAGCAATACGAGGGATTTGGGTGCCTGTTAA	RNNNNSHAIHCSNTRDLGAC*	-2.744	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22143	GLVIRTGGTLTFSSIIPTTK			20	SLAY-screened peptide P493			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCTGGTCATTCGTACTGGCGGTACGCTCACTTTTAGTAGTATCATCCCTACGACCAAGTAA	GLVIRTGGTLTFSSIIPTTK*	-2.744	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22144	HPSTWTMFTIDKSTLSFWTT			20	SLAY-screened peptide P494			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCTAGTACTTGGACTATGTTTACTATTGACAAGAGCACGCTCTCCTTCTGGACCACTTAA	HPSTWTMFTIDKSTLSFWTT*	-2.741	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22145	TRSTLLCCRSILML			14	SLAY-screened peptide P495			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCGATCAACATTGCTTTGCTGCCGTTCTATATTAATGCTTTGAGAAGCGGTTTCATTAACT	TRSTLLCCRSILML*EAVSLT	-2.74	6.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22146	HCSTRLITLATPPTSQFFNS			20	SLAY-screened peptide P496			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTGTAGTACGCGTCTCATCACTCTTGCTACCCCTCCTACGTCTCAGTTTTTTAATAGTTAA	HCSTRLITLATPPTSQFFNS*	-2.734	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22147	LRVSEESGSSCRIAGILRGMS			21	SLAY-screened peptide P497			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCGGGTATCGGAGGAGAGCGGTTCTTCCTGTCGTATTGCGGGTATACTTCGCGGGATGTCT	LRVSEESGSSCRIAGILRGMS	-2.732	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22148	FSLYIRTYTNYKTNIILYII			20	SLAY-screened peptide P498			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTCGCTGTATATCCGCACTTATACTAACTATAAGACTAATATTATTCTCTATATCATTTAA	FSLYIRTYTNYKTNIILYII*	-2.732	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22149	VPMGCSPAPWYNHFGKRSYM			20	SLAY-screened peptide P499			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCCTATGGGCTGTAGCCCTGCTCCTTGGTACAATCATTTCGGTAAGCGCAGCTATATGTAA	VPMGCSPAPWYNHFGKRSYM*	-2.731	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22150	RPCFTSVFNSPFFFHNTQQF			20	SLAY-screened peptide P500			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCTTGCTTCACTTCGGTCTTTAACAGCCCTTTTTTTTTTCATAACACGCAGCAGTTCTAA	RPCFTSVFNSPFFFHNTQQF*	-2.731	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22151	CPSARCYFCRSVNITDCNTH			20	SLAY-screened peptide P501			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCTAGTGCTCGTTGTTACTTCTGTCGTAGTGTTAATATTACGGATTGTAATACTCACTAA	CPSARCYFCRSVNITDCNTH*	-2.73	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22152	HPHPITFDFRRPHTLVPPPS			20	SLAY-screened peptide P502			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCCCACCCTATCACTTTTGATTTTCGTCGCCCCCATACTTTGGTCCCTCCGCCTTCGTAA	HPHPITFDFRRPHTLVPPPS*	-2.729	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22153	TPVYTYNLISTWPARETVYL			20	SLAY-screened peptide P503			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCCCGTTTATACGTACAATCTCATTTCCACCTGGCCGGCGCGCGAGACTGTCTACTTGTAA	TPVYTYNLISTWPARETVYL*	-2.727	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22154	QVTHGLPMAII			11	SLAY-screened peptide P504			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGTCACTCATGGGTTGCCTATGGCCATTATTTAGTTGCATCCTGCCGACGGTGCTGTCTAA	QVTHGLPMAII*LHPADGAV*	-2.727	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22155	PLVLDITWEYALARHNNNLL			20	SLAY-screened peptide P505			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTTGGTCCTGGACATTACTTGGGAGTACGCTTTGGCCCGTCACAACAATAATTTGCTTTAA	PLVLDITWEYALARHNNNLL*	-2.72	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22156	LRRGDSHFSLVNFYNNTAYY			20	SLAY-screened peptide P506			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCGCAGGGGCGACAGCCATTTCAGCCTTGTCAATTTCTATAATAATACGGCTTACTATTAA	LRRGDSHFSLVNFYNNTAYY*	-2.715	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22157	ILCRHVFPRPCDTTYSSDRD			20	SLAY-screened peptide P507			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTTTGTGCCGCCACGTTTTTCCTCGGCCCTGCGACACCACTTATAGTTCTGATAGGGATTAA	ILCRHVFPRPCDTTYSSDRD*	-2.715	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22158	RIC			3	SLAY-screened peptide P508			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGATTTGTTAGCTGCCCGTTAACTATAACGAGCGGACCGATGTCCCGAAGGTCGCTTCCTAA	RIC*LPVNYNERTDVPKVAS*	-2.712	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22159	CRHLPGVELVKISL			14	SLAY-screened peptide P509			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGCCACCTTCCTGGCGTGGAGCTTGTTAAGATTTCTTTGTAGTTCAGTACTTATGCGTAA	CRHLPGVELVKISL*FSTYA*	-2.712	5.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22160	HRLGTLRSALLLFCIYVLVR			20	SLAY-screened peptide P510			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCGCTTGGGCACTCTGCGTTCTGCGCTTCTTCTTTTCTGCATCTATGTCCTTGTTCGTTAA	HRLGTLRSALLLFCIYVLVR*	-2.711	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22161	PG			2	SLAY-screened peptide P511			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGGGTAGTACAGCTAATCCCCTGTGCCTATGTGAGTTTCACGAAGCGTTTTCATGCCTAAC	PG*YS*SPVPM*VSRSVFMPN	-2.711	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22162	FNPSLNWTNFVRPVTMVSQT			20	SLAY-screened peptide P512			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTAACCCGTCGCTTAACTGGACTAATTTTGTGCGGCCTGTTACCATGGTTAGCCAGACCTAA	FNPSLNWTNFVRPVTMVSQT*	-2.71	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22163	RPMRLKAYQPHHPPHYRWIE			20	SLAY-screened peptide P513			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCCATGCGCCTCAAGGCTTATCAGCCTCATCATCCTCCGCATTATCGTTGGATTGAGTAA	RPMRLKAYQPHHPPHYRWIE*	-2.708	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22164	YHDCAPPQLSLGDLYTLIAS			20	SLAY-screened peptide P514			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCACGACTGTGCTCCTCCCCAGTTGAGTCTTGGTGATCTTTATACGCTTATCGCGTCCTAA	YHDCAPPQLSLGDLYTLIAS*	-2.706	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22165	NPSLRIYYDNSSCRYKPWLN			20	SLAY-screened peptide P515			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCCTTCTCTTCGTATCTATTACGACAATAGTAGTTGTCGCTATAAGCCCTGGCTCAACTAA	NPSLRIYYDNSSCRYKPWLN*	-2.703	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22166	VFFWSLVLVRRLSKRQP			17	SLAY-screened peptide P516			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTTTTTTTGGTCGCTGGTCCTTGTTAGGAGGCTTTCGAAGCGTCAGCCCTAGACTCCCTAA	VFFWSLVLVRRLSKRQP*TP*	-2.702	0.03098	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22167	GASHLRAYPYIRNVTSFTLY			20	SLAY-screened peptide P517			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGGCGTCTCATCTCCGTGCTTATCCTTATATTCGGAATGTCACGTCTTTTACTCTTTATTAA	GASHLRAYPYIRNVTSFTLY*	-2.699	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22168	STLLTRNLYDYRWQCCAWSI			20	SLAY-screened peptide P518			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCACTCTGCTTACTAGGAATTTGTATGACTATCGTTGGCAGTGTTGCGCTTGGTCTATTTAA	STLLTRNLYDYRWQCCAWSI*	-2.697	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22169	TGLSNDHGDYYSQSKCGEVGY			21	SLAY-screened peptide P519			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGGTTTGTCCAATGACCATGGGGACTACTACTCTCAGTCTAAGTGTGGGGAGGTGGGCTAC	TGLSNDHGDYYSQSKCGEVGY	-2.697	3.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22170	NDKSNSYPSFDLFCDSVALP			20	SLAY-screened peptide P520			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGATAAGAGCAATTCCTACCCTAGTTTTGACTTGTTTTGCGATTCCGTGGCGCTCCCCTAA	NDKSNSYPSFDLFCDSVALP*	-2.694	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22171	DCLYLYALPASLHCYLIRHA			20	SLAY-screened peptide P521			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTGCCTGTACCTTTACGCGCTCCCTGCTTCGTTGCACTGTTACCTTATTCGTCACGCCTAA	DCLYLYALPASLHCYLIRHA*	-2.694	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22172	YRFNLGFLYVNDQLCTRTDR			20	SLAY-screened peptide P522			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCGCTTTAATCTCGGCTTCCTTTATGTCAACGATCAGCTTTGTACTCGTACTGACCGCTAA	YRFNLGFLYVNDQLCTRTDR*	-2.694	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22173	ILRYHRCTDHKQRRHGRRPI			20	SLAY-screened peptide P523			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCTTCGGTATCATCGTTGTACTGATCATAAGCAGCGGCGTCATGGGAGGCGTCCCATTTAA	ILRYHRCTDHKQRRHGRRPI*	-2.693	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22174	TLNATVNSCSVNCL			14	SLAY-screened peptide P524			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTCAACGCCACTGTTAATAGTTGTAGTGTCAATTGTCTCTAGGCCGCTCTGCGTACGTAA	TLNATVNSCSVNCL*AALRT*	-2.693	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22175	AAGRSITYSWAVLRLCPHWF			20	SLAY-screened peptide P525			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGCCGGTCGCAGCATCACCTACAGTTGGGCTGTGCTCAGGCTTTGCCCCCACTGGTTCTAA	AAGRSITYSWAVLRLCPHWF*	-2.692	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22176	LTSLFLKDSPYNSSNAPELT			20	SLAY-screened peptide P526			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACTAGCCTGTTCCTCAAGGACAGCCCTTATAATAGTTCTAATGCGCCTGAGTTGACCTAA	LTSLFLKDSPYNSSNAPELT*	-2.69	3.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22177	YTT			3	SLAY-screened peptide P527			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATACTACTTAGATGCGTACCACGTCGATTTACAATAATTGCTTTACCGCTCTTGTGCCCTAA	YTT*MRTTSIYNNCFTALVP*	-2.689	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22178	PPGVLAHVHLGLASSART			18	SLAY-screened peptide P528			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCCGGGGTGCTGGCCCATGTGCATCTGGGCCTCGCCTCGTCCGCTAGGACCTAGCAGTAA	PPGVLAHVHLGLASSART*Q*	-2.689	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22179	SSKQFQ			6	SLAY-screened peptide P529			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTCTAAGCAGTTCCAGTAGGACTCGTGCCCGAGCGGCCCCCAGCACACCTTGACGGCTTAA	SSKQFQ*DSCPSGPQHTLTA*	-2.689	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22180	TLG			3	SLAY-screened peptide P530			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTGGGGTAGGAGAGGTGGGCGACGGGCCAGCTCGCTCCCAACTCCAGGGACAACGTGTAA	TLG*ERWATGQLAPNSRDNV*	-2.686	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22181	SHRVLAPTAQLKFTLCYPGA			20	SLAY-screened peptide P531			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCATCGCGTCTTGGCGCCGACCGCCCAGCTCAAGTTCACTCTGTGTTACCCTGGTGCCTAA	SHRVLAPTAQLKFTLCYPGA*	-2.684	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22182	NTPGRPKPTHYMCSLLPAIA			20	SLAY-screened peptide P532			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACACTCCTGGCCGGCCGAAGCCTACCCATTATATGTGTAGTTTGCTTCCGGCTATCGCTTAA	NTPGRPKPTHYMCSLLPAIA*	-2.681	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22183	NRMVVTINKTTTHHV			15	SLAY-screened peptide P533			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCGTATGGTTGTGACGATCAACAAGACGACTACTCATCACGTCTAGGACGGTCTTTATTAA	NRMVVTINKTTTHHV*DGLY*	-2.68	2.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22184	LSAAPRVRVATDYNGSLPNP			20	SLAY-screened peptide P534			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTCCGCTGCTCCGAGGGTCCGTGTGGCGACTGATTATAACGGTAGCCTTCCCAACCCCTAA	LSAAPRVRVATDYNGSLPNP*	-2.68	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22185	GRYSHQMRPTSYSANSLI			18	SLAY-screened peptide P535			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCGCTATTCCCACCAGATGCGGCCTACTTCTTATTCGGCTAATTCGCTCATCTAGGGCTAA	GRYSHQMRPTSYSANSLI*G*	-2.679	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22186	TVSKDDALSLDPSSILKEMP			20	SLAY-screened peptide P536			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGTGTCTAAGGACGATGCTCTTTCGCTGGATCCTAGTAGCATTCTTAAGGAGATGCCCTAA	TVSKDDALSLDPSSILKEMP*	-2.679	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22187	PIHDYVRLSFISSCCTCAFN			20	SLAY-screened peptide P537			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATCCACGACTATGTGCGTCTTAGTTTCATCAGTAGTTGTTGCACTTGTGCGTTTAATTAA	PIHDYVRLSFISSCCTCAFN*	-2.676	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22188	LRPTSYSG			8	SLAY-screened peptide P538			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAGGCCCACTAGCTACAGCGGCTAGTTTTACAATTATTATACGCTCTCGAAGTATCTGTAA	LRPTSYSG*FYNYYTLSKYL*	-2.676	0.000314	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22189	TRLNRPTYTHTSRDTDFDFL			20	SLAY-screened peptide P539			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCGGCTTAACCGTCCGACGTACACGCACACTTCGCGCGACACTGACTTTGATTTCCTTTAA	TRLNRPTYTHTSRDTDFDFL*	-2.674	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22190	QCHPLWLWHARDPPSPCRKQ			20	SLAY-screened peptide P540			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTGCCATCCTTTGTGGCTTTGGCACGCGCGGGACCCGCCGTCTCCGTGCAGGAAGCAGTAA	QCHPLWLWHARDPPSPCRKQ*	-2.666	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22191	TD			2	SLAY-screened peptide P541			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGATTAGAACTACGGTTCGGCGTATGGCTATTCTCCGAGTTTTATGAATTGTGAGTCGTAA	TD*NYGSAYGYSPSFMNCES*	-2.666	0.000104	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22192	ARSPVTKAIHSRANYNVFPN			20	SLAY-screened peptide P542			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCGTAGCCCTGTTACCAAGGCCATTCATTCTCGTGCTAATTATAATGTGTTCCCGAACTAA	ARSPVTKAIHSRANYNVFPN*	-2.659	1.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22193	APYTH			5	SLAY-screened peptide P543			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCCTTATACTCATTAGAATACGGATAACGACTGGAATCCGAGTATCAAGCAGAGTCCGTAA	APYTH*NTDNDWNPSIKQSP*	-2.656	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22194	PTDSTVHVGDIGLFYENTSF			20	SLAY-screened peptide P544			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACTGACTCTACTGTCCACGTCGGTGACATTGGTTTGTTTTATGAGAATACTAGCTTCTAA	PTDSTVHVGDIGLFYENTSF*	-2.654	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22195	RTHFTSGFGRHCNIVCTFHF			20	SLAY-screened peptide P545			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGACGCATTTCACGTCGGGCTTTGGCAGGCACTGCAATATCGTCTGCACCTTCCACTTCTAA	RTHFTSGFGRHCNIVCTFHF*	-2.653	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22196	TTYCLRSPNAPSPNIH			16	SLAY-screened peptide P546			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGACGTATTGCCTCCGGTCTCCGAATGCTCCTTCGCCGAATATTCATTAGCATCGCCTTTAA	TTYCLRSPNAPSPNIH*HRL*	-2.653	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22197	LSRNFIIRKGLNVRPQILLC			20	SLAY-screened peptide P547			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTCTCGCAATTTCATTATCCGCAAGGGCCTTAACGTGCGTCCTCAGATCCTTCTCTGCTAA	LSRNFIIRKGLNVRPQILLC*	-2.652	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22198	PPTEQPSIPTARASPTPQDS			20	SLAY-screened peptide P548			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCCACTGAGCAGCCTTCTATCCCCACCGCCCGTGCTTCCCCGACGCCGCAGGATAGCTAA	PPTEQPSIPTARASPTPQDS*	-2.651	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22199	LHNADHRAADHRTISRHK			18	SLAY-screened peptide P549			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCACAACGCCGATCACCGCGCGGCTGATCATCGCACTATTAGTCGTCACAAGTAATGAGTA	LHNADHRAADHRTISRHK**V	-2.647	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22200	FKTCFYRDPTIACSHHCDTD			20	SLAY-screened peptide P550			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAAGACTTGTTTTTATCGTGACCCTACGATTGCGTGTTCTCACCATTGTGACACCGACTAA	FKTCFYRDPTIACSHHCDTD*	-2.646	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22201	VLLNRYFKEIGILSRFGTSL			20	SLAY-screened peptide P551			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCTCCTCAACCGCTATTTCAAGGAGATCGGTATCCTTAGTCGTTTCGGTACGAGTCTGTAA	VLLNRYFKEIGILSRFGTSL*	-2.642	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22202	ATLCSNTIGVHD			12	SLAY-screened peptide P552			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACTCTCTGCTCTAACACGATCGGGGTTCATGATTAGAATATGTACTAGCATATCTATTAA	ATLCSNTIGVHD*NMY*HIY*	-2.642	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22203	DTTLPSSNGVESPNRNIAIS			20	SLAY-screened peptide P553			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATACTACCCTCCCTTCGTCTAACGGGGTTGAGAGTCCTAATCGCAATATTGCTATTAGCTAA	DTTLPSSNGVESPNRNIAIS*	-2.641	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22204	ESDHSPSHLVLHSTHYLTHF			20	SLAY-screened peptide P554			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGAGTGATCATTCGCCTTCTCATCTTGTTCTTCATTCTACGCACTACCTGACGCATTTCTAA	ESDHSPSHLVLHSTHYLTHF*	-2.64	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22205	RSCHAQHCYWYYTLLRASIP			20	SLAY-screened peptide P555			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTCCTGCCATGCTCAGCATTGTTATTGGTATTATACTTTGCTGCGTGCCTCCATTCCTTAA	RSCHAQHCYWYYTLLRASIP*	-2.638	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22206	WGGHSMLCLLLAPRGAFAAV			20	SLAY-screened peptide P556			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGGGTGGCCATAGTATGCTCTGTCTTCTTCTCGCCCCTCGCGGTGCGTTTGCTGCTGTCTAA	WGGHSMLCLLLAPRGAFAAV*	-2.635	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22207	LIHHSHVLTDSCFFHRNGIE			20	SLAY-screened peptide P557			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCATCCATCACTCTCATGTCCTTACTGATAGCTGCTTTTTCCATCGCAATGGTATTGAGTAA	LIHHSHVLTDSCFFHRNGIE*	-2.635	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22208	CLHTPYPQNLCLNRVCNNLS			20	SLAY-screened peptide P558			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCTGCATACCCCTTATCCCCAGAATCTTTGCCTCAATCGTGTTTGTAATAATCTTAGCTAA	CLHTPYPQNLCLNRVCNNLS*	-2.632	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22209	VHIPLHSP			8	SLAY-screened peptide P559			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCACATTCCCCTCCACAGCCCCTAGAATTGCATTTACTACTACAGTGGAAGTTTTTTTAAC	VHIPLHSP*NCIYYYSGSFFN	-2.628	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22210	CEFLDALGHCHSLSGFPGNV			20	SLAY-screened peptide P560			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGAGTTCCTGGACGCTCTGGGCCATTGTCACTCGCTCTCGGGCTTTCCCGGTAATGTGTAA	CEFLDALGHCHSLSGFPGNV*	-2.628	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22211	YDHFPNFNDNYCWPPITCYL			20	SLAY-screened peptide P561			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGATCATTTTCCCAATTTCAATGACAACTATTGCTGGCCTCCTATTACTTGCTATCTGTAA	YDHFPNFNDNYCWPPITCYL*	-2.627	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22212	SASFT			5	SLAY-screened peptide P562			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGCTAGTTTTACGTAGATGCTGTCGGTTTGTGTTGAGGCGCGTACGCCCGCTATTAGGTAA	SASFT*MLSVCVEARTPAIR*	-2.627	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22213	LPSSMPHILFHCWVGLNRSN			20	SLAY-screened peptide P563			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCCTCGAGCATGCCTCACATCCTTTTTCATTGTTGGGTTGGCCTTAATAGGAGTAACTAA	LPSSMPHILFHCWVGLNRSN*	-2.625	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22214	ARWSFLFLLSTAHCPLPNRN			20	SLAY-screened peptide P564			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCGGTGGTCGTTCTTGTTTCTGTTGTCCACTGCGCACTGTCCCTTGCCGAATCGGAATTAA	ARWSFLFLLSTAHCPLPNRN*	-2.624	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22215	LPPY			4	SLAY-screened peptide P565			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCCCCTTATTAGGTTCTTTACCATTTCCATGCTCCTCGGGAGATTGAGAAGATGATCTAA	LPPY*VLYHFHAPREIEKMI*	-2.623	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22216	LYVRAAYPNLSWSVPVLRVP			20	SLAY-screened peptide P566			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTACGTCCGCGCTGCCTATCCGAATTTGTCCTGGTCTGTGCCTGTTCTTCGCGTTCCGTAA	LYVRAAYPNLSWSVPVLRVP*	-2.617	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22217	PCTRSTLSPPNFVVELVSNW			20	SLAY-screened peptide P567			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGTACTCGCTCGACCCTTAGTCCGCCCAATTTTGTGGTTGAGCTTGTTAGTAATTGGTAA	PCTRSTLSPPNFVVELVSNW*	-2.617	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22218	PDGFLRRSIPVNPSQTHFAH			20	SLAY-screened peptide P568			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGACGGTTTTCTTAGGCGTAGTATCCCTGTGAACCCGTCCCAGACGCATTTCGCTCATTAA	PDGFLRRSIPVNPSQTHFAH*	-2.617	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22219	PKRRRHFSNQLLRAVGKFDD			20	SLAY-screened peptide P569			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAAGCGCCGTCGTCATTTTAGCAATCAGCTTCTTCGCGCGGTGGGCAAGTTCGATGACTAA	PKRRRHFSNQLLRAVGKFDD*	-2.616	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22220	DNNNHLK			7	SLAY-screened peptide P570			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACAATAACAATCATCTTAAGTAGCTGTACTTGAACAAGGGTACGTTCTCCGCCGGCATCTAC	DNNNHLK*LYLNKGTFSAGIY	-2.616	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22221	LAVVASSCYTIHSHTNPPVT			20	SLAY-screened peptide P571			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGGCCGTTGTGGCTAGCAGCTGCTATACGATCCATTCCCATACTAATCCCCCTGTTACTTAA	LAVVASSCYTIHSHTNPPVT*	-2.612	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22222	LHFSRLRRL			9	SLAY-screened peptide P572			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCACTTCTCTAGATTAAGGCGCTTATGACCATGTCCAATACCTACTGCGGGTTGTCCTAAC	LHFSRLRRL*PCPIPTAGCPN	-2.605	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22223	VTSEPCASAPRGPHPLADSS			20	SLAY-screened peptide P573			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGACCAGCGAGCCGTGCGCCTCTGCGCCGCGGGGCCCGCACCCTCTCGCGGACTCCAGCTAA	VTSEPCASAPRGPHPLADSS*	-2.603	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22224	TARNPGPLLSHALCFLANTV			20	SLAY-screened peptide P574			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGCGCGGAACCCCGGTCCGCTGCTTTCTCACGCTTTGTGCTTTCTGGCCAATACCGTCTAA	TARNPGPLLSHALCFLANTV*	-2.603	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22225	QLRRENTETILSSIRILPLAN			21	SLAY-screened peptide P575			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCTCCGGCGTGAGAATACTGAGACCATCTTGTCCTCTATACGAATATTGCCCCTTGCTAAC	QLRRENTETILSSIRILPLAN	-2.602	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22226	VNFNPCPHSNISRPT			15	SLAY-screened peptide P576			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTAATTTTAATCCCTGTCCTCATTCGAATATTTCGCGCCCTACTTAGTGCTGCATTCCCTAA	VNFNPCPHSNISRPT*CCIP*	-2.602	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22227	SLSVPHLRHMLLATTTLALR			20	SLAY-screened peptide P577			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTTGTCTGTGCCTCACCTCCGGCATATGCTGCTCGCTACCACCACCCTCGCTCTGCGCTAA	SLSVPHLRHMLLATTTLALR*	-2.601	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22228	FYGVLIYLRYLFFSFAIFVF			20	SLAY-screened peptide P578			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTATGGTGTGCTTATCTACCTGCGCTACCTCTTCTTTTCCTTCGCGATCTTCGTTTTTTAA	FYGVLIYLRYLFFSFAIFVF*	-2.598	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22229	TGPLRHFVNYYNIAHDQTTT			20	SLAY-screened peptide P579			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGGCCCGCTCCGGCACTTCGTTAATTACTACAATATCGCTCATGATCAGACTACCACTTAA	TGPLRHFVNYYNIAHDQTTT*	-2.597	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22230	RRTRTPRGDAKNSAYSLGAP			20	SLAY-screened peptide P580			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAGGACTCGGACCCCGCGGGGTGATGCCAAGAATTCTGCTTATTCTCTGGGTGCTCCCTAA	RRTRTPRGDAKNSAYSLGAP*	-2.595	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22231	DLRQDLSGLRIFTISTADLCN			21	SLAY-screened peptide P581			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCTTCGGCAGGACCTCTCCGGTCTTCGCATTTTTACTATTAGCACTGCGGACTTATGTAAC	DLRQDLSGLRIFTISTADLCN	-2.594	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22232	LHHMADNNQVGDTLAEMLVS			20	SLAY-screened peptide P582			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCATCACATGGCCGACAACAATCAGGTGGGCGACACTCTTGCTGAGATGCTCGTGAGTTAA	LHHMADNNQVGDTLAEMLVS*	-2.594	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22233	HNHCHTLYNTPTILRPCACT			20	SLAY-screened peptide P583			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAATCACTGTCACACTCTGTATAATACTCCCACCATCTTGAGGCCGTGTGCCTGTACTTAA	HNHCHTLYNTPTILRPCACT*	-2.593	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22234	TFLSPLAGPLCNSSYFLASV			20	SLAY-screened peptide P584			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTTTCTGTCCCCTCTCGCCGGGCCCCTCTGCAACTCGTCCTATTTCCTGGCTTCTGTCTAA	TFLSPLAGPLCNSSYFLASV*	-2.593	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22235	SSIRTSVMDLVNYSTNFRNA			20	SLAY-screened peptide P585			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGAGTATCCGGACCAGTGTTATGGACCTTGTTAATTACTCGACTAACTTTCGTAATGCTTAA	SSIRTSVMDLVNYSTNFRNA*	-2.592	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22236	LFPAQPAGCHFISPLLPVPAN			21	SLAY-screened peptide P586			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTTCCTGCTCAGCCTGCTGGCTGCCATTTCATCAGTCCATTATTACCAGTCCCCGCTAAC	LFPAQPAGCHFISPLLPVPAN	-2.592	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22237	AVARWLRDLNAVDIADFSRS			20	SLAY-screened peptide P587			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGTGGCTCGTTGGCTTCGTGACCTCAACGCTGTTGATATTGCTGACTTCTCTAGGAGCTAA	AVARWLRDLNAVDIADFSRS*	-2.591	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22238	CLHTTLYTWILGSTFCGFLC			20	SLAY-screened peptide P588			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTGCACACTACCCTCTATACTTGGATTCTGGGTTCGACTTTCTGCGGTTTCCTTTGCTAA	CLHTTLYTWILGSTFCGFLC*	-2.591	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22239	HLHRRCRQSFFYPRLAPNRM			20	SLAY-screened peptide P589			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTTGCATCGGAGGTGCCGGCAGTCTTTTTTTTATCCTCGTTTGGCTCCCAATCGGATGTAA	HLHRRCRQSFFYPRLAPNRM*	-2.588	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22240	PTYLALATQHPNDNLGHDRR			20	SLAY-screened peptide P590			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACCTATTTGGCGTTGGCCACTCAGCACCCTAACGACAACCTGGGTCACGATCGTAGGTAA	PTYLALATQHPNDNLGHDRR*	-2.588	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22241	SVLFRVTTHTHHNKT			15	SLAY-screened peptide P591			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGTCCTTTTTCGTGTCACTACGCATACGCACCATAACAAGACCTAGGGGTAGCCGACCTAA	SVLFRVTTHTHHNKT*G*PT*	-2.587	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22242	LDSTHHYNKYMYASPL			16	SLAY-screened peptide P592			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGATAGTACGCACCATTATAATAAGTATATGTATGCTTCTCCCTTGTAGTCTAACAATTAA	LDSTHHYNKYMYASPL*SNN*	-2.586	7.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22243	TPLNYSVRNPHFTIDVPYTS			20	SLAY-screened peptide P593			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCGCTTAACTACTCGGTCCGCAATCCTCATTTTACTATTGACGTTCCCTATACTTCTTAA	TPLNYSVRNPHFTIDVPYTS*	-2.585	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22244	IIFCAMRPTSHIEPVTTSGN			20	SLAY-screened peptide P594			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTATTTTTTGCGCTATGCGGCCTACTTCTCATATCGAGCCCGTGACTACCTCGGGCAACTAA	IIFCAMRPTSHIEPVTTSGN*	-2.582	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22245	ASPPVMSSKTPSCVSNITNY			20	SLAY-screened peptide P595			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTCGCCCCCCGTGATGTCCAGCAAGACTCCTTCTTGTGTCTCTAACATCACTAACTATTAA	ASPPVMSSKTPSCVSNITNY*	-2.581	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22246	RIVNMSRPPYRIKFSVHSCD			20	SLAY-screened peptide P596			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTATCGTCAATATGTCCCGGCCTCCTTACCGTATTAAGTTTTCTGTGCACTCGTGTGATTAA	RIVNMSRPPYRIKFSVHSCD*	-2.581	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22247	INLVCI			6	SLAY-screened peptide P597			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAACCTTGTGTGCATTTAGCTTCTCACCGCTGCGCACATTTATTATGTTCGGACGTTTTAA	INLVCI*LLTAAHIYYVRTF*	-2.579	8.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22248	PSRSMHHYPSRAVLMPLLRVN			21	SLAY-screened peptide P598			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGTCGGTCTATGCATCACTATCCGTCTCGCGCCGTGTTGATGCCGCTGCTGCGAGTTAAC	PSRSMHHYPSRAVLMPLLRVN	-2.578	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22249	HRKFTSNHLNYCITNKARLLN			21	SLAY-screened peptide P599			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCGGAAGTTCACGTCTAATCATCTTAACTATTGCATTACGAATAAAGCTCGCTTGCTTAAC	HRKFTSNHLNYCITNKARLLN	-2.577	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22250	RTSIHLFT			8	SLAY-screened peptide P600			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTACTTCGATCCATCTGTTTACCTAGATGTTTAGCAGTGGTTTGGCTTCACTGTGTATTAAC	RTSIHLFT*MFSSGLASLCIN	-2.576	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22251	SAPYTLVPLSYLNCNLPTDL			20	SLAY-screened peptide P601			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCCCCTTACACCCTGGTCCCGCTTTCGTATCTTAATTGCAATCTGCCTACTGACTTGTAA	SAPYTLVPLSYLNCNLPTDL*	-2.575	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22252	TPVYPLSDL			9	SLAY-screened peptide P602			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCTGTTTACCCTTTGTCTGATCTTTAGCCCTTCATGCGCCCCTCGCGCATGAGGTGTTAA	TPVYPLSDL*PFMRPSRMRC*	-2.571	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22253	LILCVLGCPCFVL			13	SLAY-screened peptide P603			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGATCCTTTGTGTCCTGGGCTGTCCTTGTTTTGTCCTTTAGTCTACATTTTGAGGCCGTAAC	LILCVLGCPCFVL*STF*GRN	-2.57	0.000586	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22254	PNVCDALPPSRACTLSAPSR			20	SLAY-screened peptide P604			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAATGTCTGTGATGCCCTTCCTCCTTCGCGCGCGTGTACCCTGAGCGCCCCCTCTCGGTAA	PNVCDALPPSRACTLSAPSR*	-2.57	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22255	PPSVLIDGYFRPSDCSSERTN			21	SLAY-screened peptide P605			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCTTCTGTCCTTATTGACGGCTACTTCAGGCCTTCTGACTGTAGTTCCGAGAGGACTAAC	PPSVLIDGYFRPSDCSSERTN	-2.57	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22256	PH			2	SLAY-screened peptide P606			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCACTAGCGTTGGCGGCTTCTCTTCCACTATTAGGCCCCCCTTTTGTGCATCCGTTGCTAA	PH*RWRLLFHY*APLLCIRC*	-2.569	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22257	LSYHLNYPVNCSDHLHGLPC			20	SLAY-screened peptide P607			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCCTATCATCTCAATTATCCCGTTAATTGTAGTGACCACCTGCACGGTTTGCCCTGTTAA	LSYHLNYPVNCSDHLHGLPC*	-2.567	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22258	PNGTLIFCPLHSPRFLA			17	SLAY-screened peptide P608			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAATGGGACTCTTATCTTCTGTCCTCTGCATTCTCCTCGCTTTCTCGCTTAGGTGTGTTAA	PNGTLIFCPLHSPRFLA*VC*	-2.563	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22259	LAAP			4	SLAY-screened peptide P609			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGCTGCCCCTTAGTTTTCTTCTCTTGGTTCCGTGCTTATCCCGACGATCAGCTACAGCTAA	LAAP*FSSLGSVLIPTISYS*	-2.563	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22260	NLSLNSQGMARHRTDRATLS			20	SLAY-screened peptide P610			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCTGTCGCTCAATTCGCAGGGCATGGCGAGGCACCGCACGGATCGTGCTACTCTGTCGTAA	NLSLNSQGMARHRTDRATLS*	-2.563	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22261	PRSYNLGTVPPRSDPYNILN			20	SLAY-screened peptide P611			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGTTCCTACAATCTGGGTACGGTGCCTCCGCGTTCTGATCCCTACAATATTCTTAATTAA	PRSYNLGTVPPRSDPYNILN*	-2.56	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22262	LRLRSLCLFCTVFSNNDSHA			20	SLAY-screened peptide P612			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCGCCTTCGTTCTCTTTGTTTGTTTTGCACGGTTTTCTCCAATAACGATTCCCACGCGTAA	LRLRSLCLFCTVFSNNDSHA*	-2.559	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22263	NFHSRSPTNLAKTNKNPVME			20	SLAY-screened peptide P613			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTTTCATAGTCGTTCGCCGACTAATCTTGCTAAGACTAATAAGAATCCTGTCATGGAGTAA	NFHSRSPTNLAKTNKNPVME*	-2.557	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22264	SATNIRSHYLNFMVSILCMT			20	SLAY-screened peptide P614			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGCGACGAATATTAGGAGTCACTATCTCAACTTTATGGTTAGTATTCTGTGCATGACTTAA	SATNIRSHYLNFMVSILCMT*	-2.556	4.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22265	PAQVASFILRVFRHIREHMH			20	SLAY-screened peptide P615			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGCTCAGGTCGCCAGTTTCATCTTGCGCGTCTTTCGGCATATCCGGGAGCATATGCACTAA	PAQVASFILRVFRHIREHMH*	-2.553	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22266	SLTFKFNRLYFTLRAWGTFG			20	SLAY-screened peptide P616			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCTTACCTTCAAGTTCAATCGGCTGTATTTCACTCTGAGGGCCTGGGGCACCTTTGGCTAA	SLTFKFNRLYFTLRAWGTFG*	-2.553	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22267	PVQPNHCTRHASD			13	SLAY-screened peptide P617			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGTTCAGCCTAATCATTGCACTCGTCACGCTTCGGATTAGGCCACCCTTACCTATTACTAA	PVQPNHCTRHASD*ATLTYY*	-2.548	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22268	TRVPSFLRLFILRFRSRRILN			21	SLAY-screened peptide P618			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCGTGTTCCAAGTTTCCTTCGCTTGTTTATTCTACGATTCAGATCACGACGGATACTTAAC	TRVPSFLRLFILRFRSRRILN	-2.548	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22269	IFHLSNYSSIVPRWTRCYCV			20	SLAY-screened peptide P619			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTTCCATCTTTCTAATTATAGTTCTATTGTCCCCCGGTGGACCCGCTGCTATTGTGTTTAA	IFHLSNYSSIVPRWTRCYCV*	-2.547	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22270	DSRPRTQVRTNARGPPRCCR			20	SLAY-screened peptide P620			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATAGTAGGCCGAGGACCCAGGTCCGTACGAATGCTCGCGGGCCGCCTCGGTGTTGTCGTTAA	DSRPRTQVRTNARGPPRCCR*	-2.547	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22271	VCDPINTYPMPLFDMYFFFL			20	SLAY-screened peptide P621			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTGTGACCCCATTAATACCTATCCTATGCCGTTGTTTGACATGTATTTTTTCTTTCTGTAA	VCDPINTYPMPLFDMYFFFL*	-2.545	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22272	LHQRTRHHSP			10	SLAY-screened peptide P622			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCATCAGCGTACCCGGCATCACTCTCCTTAGTGCTAGCCTTTCCCTGTCCTCCACGTCTAA	LHQRTRHHSP*C*PFPVLHV*	-2.545	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22273	LFYTNYRAHEDYHNYFNTQQ			20	SLAY-screened peptide P623			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTTCTACACCAATTATCGCGCTCACGAGGACTATCACAATTATTTTAATACGCAGCAGTAA	LFYTNYRAHEDYHNYFNTQQ*	-2.542	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22274	NRRLALYPVCVCGVAS			16	SLAY-screened peptide P624			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCGTCGCCTGGCTTTGTATCCTGTTTGCGTGTGCGGTGTTGCCAGTTAGGTGAACCACTAA	NRRLALYPVCVCGVAS*VNH*	-2.541	2.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22275	RSFDDMLMPITLAFFSAVCP			20	SLAY-screened peptide P625			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAGCTTCGACGATATGCTCATGCCTATCACCCTGGCCTTTTTCTCCGCCGTGTGTCCGTAA	RSFDDMLMPITLAFFSAVCP*	-2.541	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22276	YKH			3	SLAY-screened peptide P626			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAAGCATTAGAGTAACTTTACTGACAACCCTCACCTTTCCAATTCTCAGCGCGGTCCCTAA	YKH*SNFTDNPHLSNSQRGP*	-2.537	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22277	LLRNNDLSRELINTNNQDLH			20	SLAY-screened peptide P627			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTTCGTAATAATGACCTGTCCCGGGAGCTTATTAATACCAACAATCAGGACCTGCATTAA	LLRNNDLSRELINTNNQDLH*	-2.537	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22278	PPTWFESALFFIFTILFRLVN			21	SLAY-screened peptide P628			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCACCTGGTTCGAAAGCGCCTTATTTTTCATATTTACTATACTGTTTAGATTAGTTAAC	PPTWFESALFFIFTILFRLVN	-2.535	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22279	EWVSNPTLRMLTSLDCPRTL			20	SLAY-screened peptide P629			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGTGGGTGTCTAACCCGACGCTGCGTATGCTCACTAGCTTGGATTGTCCTAGGACTCTTTAA	EWVSNPTLRMLTSLDCPRTL*	-2.534	0.000104	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22280	HNTSCRPPMDPITLDCRHKT			20	SLAY-screened peptide P630			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAACACTTCTTGTAGGCCTCCCATGGATCCCATCACGCTGGATTGCCGTCATAAGACGTAA	HNTSCRPPMDPITLDCRHKT*	-2.534	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22281	YLVHLRVAMYLKHHASHQVR			20	SLAY-screened peptide P631			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTTGGTCCATCTGCGCGTTGCGATGTATCTTAAGCACCACGCGTCTCACCAGGTGCGCTAA	YLVHLRVAMYLKHHASHQVR*	-2.531	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22282	PSRNTRSTMARTQTIRYTSR			20	SLAY-screened peptide P632			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGCAGGAATACGCGTAGCACCATGGCTCGCACTCAGACTATCCGTTATACTTCTCGGTAA	PSRNTRSTMARTQTIRYTSR*	-2.531	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22283	HRTPCRFFGVYVGVYISVTC			20	SLAY-screened peptide P633			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCGGACCCCCTGTCGCTTCTTTGGTGTCTATGTTGGTGTCTATATTTCTGTTACGTGCTAA	HRTPCRFFGVYVGVYISVTC*	-2.529	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22284	RVTCMVSTNIHSAYNPAFII			20	SLAY-screened peptide P634			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGTCACGTGCATGGTCTCTACGAACATTCACTCGGCTTACAATCCGGCTTTTATCATCTAA	RVTCMVSTNIHSAYNPAFII*	-2.528	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22285	IARTYLNHSRSPPPAVP			17	SLAY-screened peptide P635			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGCTCGCACTTATCTCAATCATTCGCGGTCTCCGCCCCCGGCTGTGCCCTAACTGAGTAAG	IARTYLNHSRSPPPAVP*LSK	-2.527	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22286	WGAYRITSSRCIGKANMYID			20	SLAY-screened peptide P636			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGGGTGCTTATCGCATTACTAGCAGTCGGTGCATTGGTAAGGCGAATATGTACATTGATTAA	WGAYRITSSRCIGKANMYID*	-2.525	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22287	PNFRQSSIPENTLHCVVVLY			20	SLAY-screened peptide P637			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAATTTTCGCCAGTCTTCCATCCCGGAGAATACGCTGCATTGCGTTGTTGTTCTGTACTAA	PNFRQSSIPENTLHCVVVLY*	-2.524	3.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22288	DHDNFLEQVYYPRNRYASNS			20	SLAY-screened peptide P638			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCATGACAATTTCCTCGAGCAGGTTTATTACCCGCGTAATCGGTATGCTAGTAATTCTTAA	DHDNFLEQVYYPRNRYASNS*	-2.524	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22289	LARDGNYFGVRNTNLFSAHT			20	SLAY-screened peptide P639			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGCCCGTGACGGGAACTACTTTGGCGTGCGCAATACTAATCTTTTCAGCGCGCATACGTAA	LARDGNYFGVRNTNLFSAHT*	-2.524	0.000237	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22290	FGTSWSISYKRNFNVYRYKS			20	SLAY-screened peptide P640			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGGCACCAGCTGGAGTATTAGTTATAAGCGCAACTTTAACGTTTACAGGTATAAGAGTTAA	FGTSWSISYKRNFNVYRYKS*	-2.52	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22291	LTYNTPIYYHVHIKSGRYDM			20	SLAY-screened peptide P641			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACCTATAATACGCCTATCTATTATCATGTGCACATTAAGTCTGGGCGGTACGATATGTAA	LTYNTPIYYHVHIKSGRYDM*	-2.519	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22292	EPCRSAAAWPNI			12	SLAY-screened peptide P642			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCCTTGTAGGTCTGCTGCTGCTTGGCCTAACATTTAGGATTGCTCGGGTCACACGACTTAA	EPCRSAAAWPNI*DCSGHTT*	-2.519	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22293	PRSAPTPAVYTSPALASAST			20	SLAY-screened peptide P643			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGCTCCGCCCCCACGCCTGCCGTTTACACCTCGCCTGCGCTGGCGTCCGCTAGCACGTAA	PRSAPTPAVYTSPALASAST*	-2.519	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22294	NLCFSSLDSFITAAL			15	SLAY-screened peptide P644			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCTCTGCTTCTCTTCGCTTGACAGTTTCATTACGGCTGCGCTTTAGATCTATGATTACTAA	NLCFSSLDSFITAAL*IYDY*	-2.518	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22295	PNRVHDPCSMYTVYRKFHHS			20	SLAY-screened peptide P645			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAATCGTGTTCATGATCCGTGTAGCATGTACACGGTGTATCGCAAGTTCCATCACTCTTAA	PNRVHDPCSMYTVYRKFHHS*	-2.516	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22296	NIFSSCTILSRCGCNLIVEN			20	SLAY-screened peptide P646			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACATTTTCTCTTCTTGCACGATTCTTAGCCGTTGCGGTTGTAACCTCATTGTCGAGAATTAA	NIFSSCTILSRCGCNLIVEN*	-2.516	0.000344	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22297	CNFALPKLSTILHRLRSSLFN			21	SLAY-screened peptide P647			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAACTTCGCGCTCCCCAAGCTTTCCACTATCTTGCACCGATTGCGATCTTCACTCTTTAAC	CNFALPKLSTILHRLRSSLFN	-2.516	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22298	AHRAMGSLQGFFYTFYFLIP			20	SLAY-screened peptide P648			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCACCGTGCCATGGGTTCGCTCCAGGGTTTCTTTTACACTTTTTACTTTCTTATCCCCTAA	AHRAMGSLQGFFYTFYFLIP*	-2.515	0.000288	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22299	ISRRSTHNSDDYYRAPNISL			20	SLAY-screened peptide P649			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAGCCGTCGTAGCACTCATAATTCCGATGACTATTATAGGGCTCCTAACATCAGTCTGTAA	ISRRSTHNSDDYYRAPNISL*	-2.513	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22300	PLKVPNASNNLVRFTSPA			18	SLAY-screened peptide P650			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTAAGGTCCCTAATGCTTCGAATAACCTTGTTAGGTTTACTTCCCCGGCTTAGACGTAA	PLKVPNASNNLVRFTSPA*T*	-2.513	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22301	KPTGATHPLYSCRHTPHVNA			20	SLAY-screened peptide P651			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCCGACTGGTGCTACCCATCCTCTCTATTCTTGCAGGCATACTCCTCATGTGAATGCCTAA	KPTGATHPLYSCRHTPHVNA*	-2.513	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22302	YPSWQANAN			9	SLAY-screened peptide P652			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCCTAGCTGGCAGGCCAACGCGAATTAGAGTGATATTATTCTTGATTAGAATCATGATTAA	YPSWQANAN*SDIILD*NHD*	-2.512	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22303	RDRSLDLFCLSVHPQWDGHT			20	SLAY-screened peptide P653			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGACCGCAGTCTGGATTTGTTTTGTCTTTCGGTGCACCCCCAGTGGGACGGGCATACCTAA	RDRSLDLFCLSVHPQWDGHT*	-2.512	0.000172	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22304	SKYRVTPLAFFALYHHVTFS			20	SLAY-screened peptide P654			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCAAGTACCGCGTTACGCCTCTGGCTTTTTTCGCCCTTTACCACCACGTCACCTTCTCCTAA	SKYRVTPLAFFALYHHVTFS*	-2.512	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22305	ASISNSVAINYPHAHFPLLAN			21	SLAY-screened peptide P655			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAGTATCAGTAACTCGGTGGCTATTAATTATCCTCATGCTCACTTTCCCTTGCTGGCTAAC	ASISNSVAINYPHAHFPLLAN	-2.51	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22306	RLSLGLYNANSYTIWDVKYM			20	SLAY-screened peptide P656			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTCTCCCTCGGTCTTTATAATGCTAATAGTTACACCATTTGGGATGTCAAGTATATGTAA	RLSLGLYNANSYTIWDVKYM*	-2.508	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22307	YKTARLTDATAFLSPCSYHT			20	SLAY-screened peptide P657			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAAGACGGCCCGTCTCACTGATGCTACTGCGTTTTTGTCTCCTTGTTCTTATCATACCTAA	YKTARLTDATAFLSPCSYHT*	-2.506	4.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22308	RQHPQHACDPD			11	SLAY-screened peptide P658			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCAGCATCCCCAGCATGCCTGTGACCCCGATTAGAATCCTAGTCTGTATTGTAACGTTTAA	RQHPQHACDPD*NPSLYCNV*	-2.505	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22309	TLIFFACQIFVLPGSAHFRVS			21	SLAY-screened peptide P659			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTGATTTTTTTTGCTTGTCAGATTTTTGTCTTGCCCGGTAGTGCTCACTTTAGAGTAAGT	TLIFFACQIFVLPGSAHFRVS	-2.504	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22310	YSPNSQEGTCATNTHHILIL			20	SLAY-screened peptide P660			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTCCCCTAACTCTCAGGAGGGTACGTGTGCTACTAACACGCATCATATCCTGATCCTTTAA	YSPNSQEGTCATNTHHILIL*	-2.502	0.000177	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22311	LLHTLSEHPFFDINVCDSAS			20	SLAY-screened peptide P661			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCTCCATACCCTCTCGGAGCATCCCTTCTTTGACATTAACGTTTGTGATAGTGCTTCTTAA	LLHTLSEHPFFDINVCDSAS*	-2.501	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22312	LPTTVYLSVCPSTGGILVPH			20	SLAY-screened peptide P662			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCTACGACTGTGTACTTGTCTGTTTGCCCTTCTACTGGCGGCATTCTTGTCCCTCACTAA	LPTTVYLSVCPSTGGILVPH*	-2.501	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22313	MIHFWVLPGRLLFIG			15	SLAY-screened peptide P663			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGATTCACTTTTGGGTCCTACCGGGCCGTTTGCTCTTCATAGGTTGAATAAGCACCTGTAAC	MIHFWVLPGRLLFIG*ISTCN	-2.5	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22314	HLTSYDR			7	SLAY-screened peptide P664			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCTCACGTCGTACGATCGCTAGACTGATTGTGACAGCTTTAACGACAATTTTGACTCCTAA	HLTSYDR*TDCDSFNDNFDS*	-2.5	0.001751	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22315	SDRRVMLSFSFSDRPGVDLQA			21	SLAY-screened peptide P665			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGACCGCCGCGTTATGCTCTCTTTCTCGTTTTCTGACCGCCCTGGTGTCGACCTGCAGGCA	SDRRVMLSFSFSDRPGVDLQA	-2.499	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22316	PCHHKIRRKCTLVHRPPNAL			20	SLAY-screened peptide P666			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGCCATCACAAGATTCGCCGCAAGTGTACGCTTGTCCATAGGCCGCCCAACGCTCTCTAA	PCHHKIRRKCTLVHRPPNAL*	-2.498	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22317	HRSLHMYRNFCFNFDCE			17	SLAY-screened peptide P667			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCGTAGCCTGCACATGTATCGTAATTTTTGCTTTAATTTTGACTGTGAGTAGTACCAGTAA	HRSLHMYRNFCFNFDCE*YQ*	-2.497	0.000281	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22318	ILFPARILRLLKNFYYLKHN			20	SLAY-screened peptide P668			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCTTTTTCCGGCGCGCATCTTGCGCTTGCTTAAGAATTTTTATTACCTCAAGCATAACTAA	ILFPARILRLLKNFYYLKHN*	-2.497	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22319	TATAHKKRNNPLLTVAMGVV			20	SLAY-screened peptide P669			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGCTACGGCTCATAAGAAGCGGAACAATCCCCTTTTGACGGTGGCTATGGGCGTGGTCTAA	TATAHKKRNNPLLTVAMGVV*	-2.495	0.000297	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22320	LLLHRLLYGNHCMLTHDTSC			20	SLAY-screened peptide P670			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTTGCTGCATCGCCTCTTGTATGGGAATCATTGCATGCTTACGCACGACACTAGTTGCTAA	LLLHRLLYGNHCMLTHDTSC*	-2.495	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22321	AVPACPCLTVPDRDVPSNTV			20	SLAY-screened peptide P671			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGTGCCGGCTTGCCCTTGCCTTACTGTTCCGGACCGCGATGTTCCGAGTAATACTGTGTAA	AVPACPCLTVPDRDVPSNTV*	-2.494	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22322	PHASRLHGAYDQRFSCYNPSN			21	SLAY-screened peptide P672			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCACGCCTCGCGCCTGCATGGCGCTTATGATCAGCGCTTCTCTTGTTATAATCCTTCTAAC	PHASRLHGAYDQRFSCYNPSN	-2.493	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22323	PLAQNTDILCINYFVISTPM			20	SLAY-screened peptide P673			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTTGCCCAGAACACTGATATCCTTTGCATCAATTACTTCGTCATCAGTACGCCTATGTAA	PLAQNTDILCINYFVISTPM*	-2.489	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22324	IGERPAKTLTAGHDGGYTLAN			21	SLAY-screened peptide P674			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGGCGAGCGGCCGGCGAAGACGCTTACCGCGGGCCATGACGGGGGCTATACGCTTGCTAAC	IGERPAKTLTAGHDGGYTLAN	-2.489	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22325	SVVSAPRDRYRAPSNPRSYG			20	SLAY-screened peptide P675			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGTCGTCTCTGCGCCTCGCGATCGTTATCGGGCTCCGTCTAATCCCAGGTCCTACGGTTAA	SVVSAPRDRYRAPSNPRSYG*	-2.488	0.000102	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22326	ECSATYAVPGDQYPNYFILL			20	SLAY-screened peptide P676			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGTGCTCGGCTACTTATGCCGTCCCTGGTGACCAGTACCCTAACTACTTTATTCTCCTTTAA	ECSATYAVPGDQYPNYFILL*	-2.487	2.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22327	FPYRICYNRLSFNSHLHDAT			20	SLAY-screened peptide P677			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCCTTATCGGATCTGCTACAATCGCCTTTCTTTTAACTCGCATCTTCACGATGCGACCTAA	FPYRICYNRLSFNSHLHDAT*	-2.487	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22328	PLNPDSNPEHASLCHSEVFY			20	SLAY-screened peptide P678			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTCAACCCTGACTCTAACCCTGAGCATGCTTCGCTTTGCCACAGTGAGGTCTTCTACTAA	PLNPDSNPEHASLCHSEVFY*	-2.486	2.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22329	SRIECTPLSNVGLDPGCALN			20	SLAY-screened peptide P679			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCGTATCGAGTGCACCCCTCTGTCTAATGTTGGGCTTGATCCTGGTTGCGCTTTGAATTAA	SRIECTPLSNVGLDPGCALN*	-2.486	0.001213	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22330	CVLEINVNHYWHHREALFNI			20	SLAY-screened peptide P680			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGTTCTCGAAATTAATGTCAATCACTACTGGCATCACCGTGAGGCGCTCTTCAATATTTAA	CVLEINVNHYWHHREALFNI*	-2.485	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22331	IPRSMCPADSNVQDKGHSGP			20	SLAY-screened peptide P681			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCCGCGTAGCATGTGCCCTGCTGACTCCAATGTCCAGGACAAGGGTCACAGCGGCCCCTAA	IPRSMCPADSNVQDKGHSGP*	-2.485	0.000198	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22332	ANTMAKIHCNKVLGAIPHVL			20	SLAY-screened peptide P682			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAATACCATGGCCAAGATTCATTGTAATAAGGTGCTTGGGGCTATTCCGCATGTTCTGTAA	ANTMAKIHCNKVLGAIPHVL*	-2.483	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22333	HPFHIYCRSDSSNRRLACGN			20	SLAY-screened peptide P683			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCGTTTCACATTTATTGTCGCAGTGACAGCAGCAATCGCCGACTCGCTTGTGGTAACTGA	HPFHIYCRSDSSNRRLACGN*	-2.479	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22334	PYQSKLYHNLHRSNLCVHGD			20	SLAY-screened peptide P684			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTATCAGTCCAAGCTGTACCATAACCTTCATCGTTCGAATCTGTGTGTGCATGGGGACTAA	PYQSKLYHNLHRSNLCVHGD*	-2.479	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22335	SSPARGDLDFCRTFNNIQIT			20	SLAY-screened peptide P685			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTCCCCCGCCCGTGGCGATCTGGACTTTTGCCGTACGTTTAATAATATCCAGATCACCTAA	SSPARGDLDFCRTFNNIQIT*	-2.478	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22336	GSACTSQFPHFTLINGHGTN			20	SLAY-screened peptide P686			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTCTGCTTGCACGAGCCAGTTCCCGCACTTTACTCTCATCAACGGCCATGGTACTAATTAA	GSACTSQFPHFTLINGHGTN*	-2.478	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22337	TMHYYKSHTLYHSNTGPTHY			20	SLAY-screened peptide P687			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCATGCATTATTATAAGAGCCACACTTTGTACCACAGCAATACCGGCCCCACTCACTACTAA	TMHYYKSHTLYHSNTGPTHY*	-2.477	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22338	DRNIPIRFVCGHNHGPLIFN			20	SLAY-screened peptide P688			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCGTAATATCCCCATCCGGTTCGTTTGTGGTCACAACCACGGGCCTCTTATTTTTAATTAA	DRNIPIRFVCGHNHGPLIFN*	-2.477	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22339	LRWAPSYSRRDFRLKFGDIR			20	SLAY-screened peptide P689			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCGGTGGGCCCCTAGTTATTCTCGTAGGGATTTCCGCCTGAAGTTCGGGGACATTCGTTAA	LRWAPSYSRRDFRLKFGDIR*	-2.476	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22340	LPRYVNTIPDISCTIPRRSVN			21	SLAY-screened peptide P690			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCGCGTTACGTTAACACTATCCCTGACATATCCTGTACCATTCCACGACGGTCAGTTAAC	LPRYVNTIPDISCTIPRRSVN	-2.476	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22341	HETPYHHRALAPVPASLLFPE			21	SLAY-screened peptide P691			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGAGACGCCTTATCACCACAGGGCCCTAGCACCAGTGCCTGCGTCACTACTTTTCCCCGAG	HETPYHHRALAPVPASLLFPE	-2.472	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22342	HRNTLRLHVGLKACVTLFNN			20	SLAY-screened peptide P692			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAGGAACACGCTCCGTCTGCACGTGGGGCTTAAGGCGTGTGTTACGCTTTTTAATAATTAA	HRNTLRLHVGLKACVTLFNN*	-2.472	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22343	TRSYKSRHMGGYISISIITFN			21	SLAY-screened peptide P693			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCGCAGTTATAAGTCTCGGCACATGGGCGGTTATATTTCTATTTCGATCATAACATTTAAC	TRSYKSRHMGGYISISIITFN	-2.469	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22344	FTHPSYRRSHCVRLASLGMN			20	SLAY-screened peptide P694			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCACTCACCCGTCTTATCGCCGCAGTCATTGTGTTCGGTTGGCTAGTTTGGGTATGAACTAA	FTHPSYRRSHCVRLASLGMN*	-2.469	0.000144	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22345	TLHGIFTFFVAGSLGVLS			18	SLAY-screened peptide P695			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTTGCACGGTATTTTTACGTTCTTTGTGGCCGGCTCTCTGGGAGTTTTGAGTTGAACTAAC	TLHGIFTFFVAGSLGVLS*TN	-2.469	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22346	LRLTAHIHLGTYPIVDVTSY			20	SLAY-screened peptide P696			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCGGCTCACCGCCCACATTCATCTTGGTACCTACCCCATTGTTGACGTTACCAGCTACTAA	LRLTAHIHLGTYPIVDVTSY*	-2.467	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22347	PVFALRSAVKSAAST			15	SLAY-screened peptide P697			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGTGTTTGCCCTGCGTAGCGCTGTTAAAAGCGCTGCGAGCACTTGAACTTTAGGACGTAAC	PVFALRSAVKSAAST*TLGRN	-2.466	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22348	HRIEFFGHSCTNLCDYYHGS			20	SLAY-screened peptide P698			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCGCATCGAGTTTTTCGGTCATTCTTGTACTAACCTGTGCGACTATTATCACGGGAGCTAA	HRIEFFGHSCTNLCDYYHGS*	-2.466	0.000107	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22349	FVDHIACPHSSPFYSIIFRI			20	SLAY-screened peptide P699			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGTTGACCATATTGCTTGCCCCCACTCGTCCCCTTTTTATAGTATTATTTTTAGGATCTAA	FVDHIACPHSSPFYSIIFRI*	-2.465	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22350	PGVSTVFCVNAHSSYFRFCR			20	SLAY-screened peptide P700			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGTGTCTCGACTGTCTTCTGTGTGAACGCTCATAGTTCCTATTTTCGTTTTTGTCGGTAA	PGVSTVFCVNAHSSYFRFCR*	-2.464	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22351	PWSVGALRAYWGHGGPRPDE			20	SLAY-screened peptide P701			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGGTCTGTCGGCGCTTTGCGTGCTTATTGGGGCCATGGTGGGCCGCGTCCTGACGAGTAA	PWSVGALRAYWGHGGPRPDE*	-2.463	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22352	LHFILDASRVCHHRKGN			17	SLAY-screened peptide P702			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCATTTCATTCTTGACGCTTCTCGGGTCTGTCACCATAGGAAGGGCAATTAGCAGACTTAA	LHFILDASRVCHHRKGN*QT*	-2.462	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22353	SSTNHHKCTRLKSNNVIMAG			20	SLAY-screened peptide P703			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCAGTACTAATCACCATAAGTGTACTCGCCTTAAGAGCAATAATGTTATCATGGCGGGTTAA	SSTNHHKCTRLKSNNVIMAG*	-2.46	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22354	RYLGQSNNSCCAASGLPINT			20	SLAY-screened peptide P704			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTATTTGGGTCAGTCCAATAATTCGTGCTGTGCTGCGTCGGGTCTCCCTATCAACACTTAA	RYLGQSNNSCCAASGLPINT*	-2.459	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22355	WRIVLCPKLHDLLYNNMHCN			20	SLAY-screened peptide P705			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCGTATTGTCCTGTGTCCCAAGCTGCACGACCTCCTTTACAATAATATGCATTGCAATTAA	WRIVLCPKLHDLLYNNMHCN*	-2.458	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22356	SCAAPSCTSYPRDKITPYSW			20	SLAY-screened peptide P706			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTGTGCCGCTCCGTCTTGTACCTCTTATCCGAGGGACAAGATCACTCCTTATTCGTGGTAA	SCAAPSCTSYPRDKITPYSW*	-2.456	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22357	VYSLGSNPDNYN			12	SLAY-screened peptide P707			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTACTCCTTGGGGTCGAACCCTGACAATTATAATTAGACTTGTATCCGCATTCTTACTTAA	VYSLGSNPDNYN*TCIRILT*	-2.456	2.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22358	YTPALCPGLSSNRVNRSSAQ			20	SLAY-screened peptide P708			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACTCCTGCGCTGTGTCCTGGGCTCTCTAGTAATAGGGTTAATCGCTCTTCTGCCCAGTAA	YTPALCPGLSSNRVNRSSAQ*	-2.456	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22359	GAEGLSVLTVNIFTKYCRHG			20	SLAY-screened peptide P709			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGCCGAGGGTTTGTCCGTTCTTACTGTTAATATCTTTACCAAGTATTGTAGGCATGGGTAA	GAEGLSVLTVNIFTKYCRHG*	-2.455	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22360	PSNPNHLVNSSDVVHCYYPR			20	SLAY-screened peptide P710			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTCCAACCCCAACCATCTTGTCAATTCGTCGGATGTTGTGCACTGCTACTATCCGCGCTAA	PSNPNHLVNSSDVVHCYYPR*	-2.455	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22361	AGWTLHTVMRAHTPTDCAYN			20	SLAY-screened peptide P711			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGGTTGGACTCTTCATACTGTTATGCGTGCCCATACCCCGACTGATTGCGCTTATAACTAA	AGWTLHTVMRAHTPTDCAYN*	-2.451	3.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22362	AACCRSQNVSLNLLFTFNRY			20	SLAY-screened peptide P712			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGCGTGTTGTAGGTCTCAGAATGTCTCTCTTAATCTTCTCTTTACGTTTAACCGGTACTAA	AACCRSQNVSLNLLFTFNRY*	-2.45	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22363	PLTDWWTSL			9	SLAY-screened peptide P713			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTGACTGATTGGTGGACTTCTCTCTAGTCGGCTACGGGCAATTATGTGCACGCGCTTTAA	PLTDWWTSL*SATGNYVHAL*	-2.45	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22364	GFGAAPWDPVSSY			13	SLAY-screened peptide P714			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTTCGGCGCTGCGCCGTGGGATCCCGTTAGTTCGTATTAGAACCATAATACTGCTGATTAA	GFGAAPWDPVSSY*NHNTAD*	-2.448	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22365	ILWLRFRGTIIIWKFRFRLVN			21	SLAY-screened peptide P715			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCTGTGGCTGCGTTTTCGGGGGACTATTATCATTTGGAAATTTAGGTTCCGTCTAGTTAAC	ILWLRFRGTIIIWKFRFRLVN	-2.442	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22366	SHAIHMAHSHFYCVSHENTS			20	SLAY-screened peptide P716			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCATGCCATCCATATGGCTCATTCGCATTTTTACTGTGTTAGCCATGAGAATACTAGTTAA	SHAIHMAHSHFYCVSHENTS*	-2.442	6.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22367	GPWSRHIYYSLIFYSIIVAA			20	SLAY-screened peptide P717			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCCTTGGAGTAGGCACATCTATTATAGCCTCATTTTCTATAGCATTATTGTTGCCGCCTAA	GPWSRHIYYSLIFYSIIVAA*	-2.441	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22368	QNQAC			5	SLAY-screened peptide P718			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGAACCAGGCTTGCTAGCATTTTGTCAACCCTATTGCTGTCCGTCTGATTCATAACTCCTAA	QNQAC*HFVNPIAVRLIHNS*	-2.44	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22369	MYLS			4	SLAY-screened peptide P719			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTACCTGTCCTAGTCTCTCCACAAGGTTGATATTAGTTCTCCTATTTCCCTGCTTTCGTAA	MYLS*SLHKVDISSPISLLS*	-2.439	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22370	VSMQRPNGKFNITV			14	SLAY-screened peptide P720			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTCTATGCAGAGGCCTAACGGTAAGTTCAACATCACTGTTTAGGATCCGGTTTAGTGTTAA	VSMQRPNGKFNITV*DPV*C*	-2.439	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22371	RAHCMPYYLFSTLTPEVILL			20	SLAY-screened peptide P721			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCTCATTGCATGCCTTATTATTTGTTTAGTACTCTCACCCCTGAGGTCATTCTGCTTTAA	RAHCMPYYLFSTLTPEVILL*	-2.439	0.000386	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22372	GYHTHSRAPHELDYRIISAI			20	SLAY-screened peptide P722			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTACCATACTCATTCGCGGGCGCCCCATGAGCTTGACTATCGGATTATCAGCGCGATTTAA	GYHTHSRAPHELDYRIISAI*	-2.438	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22373	LFFAPK			6	SLAY-screened peptide P723			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTTTTTCGCGCCGAAGTAGAACCTGAACGCTTTGCACGGTAAGTATCCTCTCATGGATTAA	LFFAPK*NLNALHGKYPLMD*	-2.437	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22374	SF			2	SLAY-screened peptide P724			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTTCTAGGTGCGGCCCTTGGTTATCCCGCTTTTTGCTCCGTCGCCCATGGCGTACTCTTAA	SF*VRPLVIPLFAPSPMAYS*	-2.436	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22375	LSGLCFAIIGCWNTY			15	SLAY-screened peptide P725			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCTGGGCTTTGTTTTGCGATTATCGGTTGTTGGAATACTTACTAGCTTTCCTGTGTTTAA	LSGLCFAIIGCWNTY*LSCV*	-2.435	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22376	TVSSTSSGSYVDGSNFVYLF			20	SLAY-screened peptide P726			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGTTTCTTCTACCTCGAGTGGCTCGTATGTCGATGGCAGTAATTTTGTCTATCTGTTTTAA	TVSSTSSGSYVDGSNFVYLF*	-2.435	0.000669	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22377	TPYHCVHRPTRWQRCCRDPP			20	SLAY-screened peptide P727			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCGTATCACTGTGTTCACCGGCCCACGAGGTGGCAGCGTTGCTGTCGTGACCCTCCCTAA	TPYHCVHRPTRWQRCCRDPP*	-2.435	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22378	PQHPCPLPPIYYMPAMLTPL			20	SLAY-screened peptide P728			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCAGCACCCTTGTCCGTTGCCGCCCATTTATTACATGCCTGCGATGCTTACTCCGCTGTAA	PQHPCPLPPIYYMPAMLTPL*	-2.433	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22379	VAEHPHLRHDGHCGVFYLTA			20	SLAY-screened peptide P729			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGCCGAGCATCCCCACCTGCGCCACGATGGTCACTGCGGCGTCTTTTATCTCACCGCCTAA	VAEHPHLRHDGHCGVFYLTA*	-2.431	0.005318	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22380	IYPPIGYPVYL			11	SLAY-screened peptide P730			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTATCCTCCGATTGGTTATCCGGTGTATCTGTAGAATACTGAGTCCATCGATTATATGTAA	IYPPIGYPVYL*NTESIDYM*	-2.43	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22381	HATGTRIHAFSPIPVRTHIP			20	SLAY-screened peptide P731			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGCGACTGGCACTAGGATTCACGCTTTCTCGCCTATTCCGGTCCGTACGCATATTCCCTAA	HATGTRIHAFSPIPVRTHIP*	-2.426	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22382	PSIQICYPLLCNTYLSNTRN			20	SLAY-screened peptide P732			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAGCATCCAGATTTGTTACCCCTTGCTTTGCAATACTTATCTGTCCAACACTAGGAATTAA	PSIQICYPLLCNTYLSNTRN*	-2.426	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22383	RTNFDLTHWSQGHWIAYPLA			20	SLAY-screened peptide P733			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGACGAACTTTGATCTTACGCACTGGTCCCAGGGTCACTGGATTGCTTATCCTCTTGCCTAA	RTNFDLTHWSQGHWIAYPLA*	-2.426	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22384	GRDHILDLDFNGGSSARNDI			20	SLAY-screened peptide P734			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCAGGGACCATATTTTGGACCTGGATTTTAACGGTGGTTCTTCTGCGCGTAATGATATTTAA	GRDHILDLDFNGGSSARNDI*	-2.425	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22385	SVRNGTIAPCVSRPSAWRPTN			21	SLAY-screened peptide P735			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGTTCGTAATGGTACGATAGCCCCGTGCGTATCAAGGCCGTCTGCTTGGCGGCCCACTAAC	SVRNGTIAPCVSRPSAWRPTN	-2.423	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22386	FPTGVYSPVYCPISNCTFDY			20	SLAY-screened peptide P736			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCCGACCGGCGTTTATTCCCCTGTTTACTGCCCCATTAGTAATTGTACTTTTGATTATTAA	FPTGVYSPVYCPISNCTFDY*	-2.419	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22387	LLLMFFSLLLLLVAVADVLTE			21	SLAY-screened peptide P737			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTGCTCATGTTCTTTTCTCTCTTGTTGTTATTGGTCGCCGTCGCAGACGTTCTAACTGAG	LLLMFFSLLLLLVAVADVLTE	-2.416	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22388	GSRRCLPCHDTLPNPTEPSS			20	SLAY-screened peptide P738			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGAGTAGGCGTTGTCTTCCCTGTCATGATACGTTGCCGAATCCCACTGAGCCGAGTTCTTAA	GSRRCLPCHDTLPNPTEPSS*	-2.415	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22389	LSCDPVYALAIKHPVILDYT			20	SLAY-screened peptide P739			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAGTTGTGACCCTGTGTATGCCCTCGCTATTAAGCATCCTGTTATTCTCGACTATACGTAA	LSCDPVYALAIKHPVILDYT*	-2.412	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22390	SYNMTSICIFVERCPSAIRVN			21	SLAY-screened peptide P740			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTACAATATGACTTCTATCTGCATCTTTGTGGAAAGATGCCCGAGTGCTATTCGCGTTAAC	SYNMTSICIFVERCPSAIRVN	-2.408	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22391	SSQYDSCRNLFNWLSMPVNI			20	SLAY-screened peptide P741			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCAGCCAGTATGATTCGTGCCGTAACTTGTTTAACTGGCTTTCTATGCCTGTGAATATTTAA	SSQYDSCRNLFNWLSMPVNI*	-2.408	3.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22392	ASSHYNRGSYLAFPS			15	SLAY-screened peptide P742			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTAGTAGTCACTACAATCGCGGTAGTTATCTCGCTTTTCCTTCCTAGACGGTCAGGAATTAA	ASSHYNRGSYLAFPS*TVRN*	-2.408	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22393	PAEGHHLHGNTSVSKMPRTL			20	SLAY-screened peptide P743			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCCGAGGGCCACCACCTGCATGGGAACACCAGCGTTTCCAAGATGCCGCGCACTCTGTAA	PAEGHHLHGNTSVSKMPRTL*	-2.406	0.000527	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22394	GPLLAGYTFGNTSGSFRYGL			20	SLAY-screened peptide P744			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCCCTTGCTCGCGGGGTACACTTTCGGTAACACTTCTGGCTCGTTTCGTTACGGGTTGTAA	GPLLAGYTFGNTSGSFRYGL*	-2.405	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22395	KQGYCGWPAVTYFPLSFSFH			20	SLAY-screened peptide P745			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCAGGGTTATTGTGGTTGGCCTGCGGTCACTTACTTTCCTCTCAGCTTCTCTTTCCATTAA	KQGYCGWPAVTYFPLSFSFH*	-2.405	0.00035	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22396	SYRATAIQVAGINVNNA			17	SLAY-screened peptide P746			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTACCGGGCCACTGCTATCCAGGTTGCTGGCATTAATGTTAATAATGCTTAGTAGACCTAA	SYRATAIQVAGINVNNA**T*	-2.405	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22397	SCTGWSLPSYGHPAFISPNS			20	SLAY-screened peptide P747			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTGTACTGGCTGGTCGCTCCCTTCTTACGGCCATCCTGCTTTTATTAGTCCTAATTCTTAA	SCTGWSLPSYGHPAFISPNS*	-2.405	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22398	IEHPFCLSMPAYNVNKVYEC			20	SLAY-screened peptide P748			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGAGCATCCCTTCTGTCTGTCGATGCCGGCTTACAATGTTAATAAGGTCTATGAGTGTTAA	IEHPFCLSMPAYNVNKVYEC*	-2.404	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22399	ADYCRPFT			8	SLAY-screened peptide P749			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGACTACTGTAGGCCCTTTACCTAGAAGATTAAGACTCATCATTTGTAGATTAGGGTTTAA	ADYCRPFT*KIKTHHL*IRV*	-2.403	3.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22400	KLYHNTYIIHCRSTLVPCNL			20	SLAY-screened peptide P750			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCTTTATCATAATACCTACATTATCCATTGCCGCTCTACGCTTGTCCCTTGCAATCTGTAA	KLYHNTYIIHCRSTLVPCNL*	-2.402	6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22401	PFLHFSFPRRFITVEHRSSC			20	SLAY-screened peptide P751			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTTCTCCATTTCTCTTTCCCTCGCCGTTTTATTACCGTGGAGCATAGGTCCAGCTGCTAA	PFLHFSFPRRFITVEHRSSC*	-2.402	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22402	HGILHPPLYSVNNSQVGWMCN			21	SLAY-screened peptide P752			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGGTATCTTGCACCCTCCCCTCTATTCTGTCAATAACAGTCAGGTTGGGTGGATGTGTAAC	HGILHPPLYSVNNSQVGWMCN	-2.4	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22403	HYHYRTAHDSKSNLLDLLTG			20	SLAY-screened peptide P753			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTACCACTACCGCACTGCTCATGATTCTAAGTCTAACTTGCTCGATTTGCTCACTGGTTAA	HYHYRTAHDSKSNLLDLLTG*	-2.4	0.000211	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22404	THPNLPLRAPLLPEAAQTNA			20	SLAY-screened peptide P754			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCATCCGAACCTCCCCTTGCGTGCCCCCCTTCTTCCGGAGGCGGCGCAGACTAATGCTTAA	THPNLPLRAPLLPEAAQTNA*	-2.399	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22405	LSLYLIVSPSETC			13	SLAY-screened peptide P755			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCGCTGTATCTTATCGTTTCCCCTTCGGAGACGTGCTAGAATGATCTTCGCAATCTGTAA	LSLYLIVSPSETC*NDLRNL*	-2.398	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22406	NRLDLRSIYKPHNFDIYFIY			20	SLAY-screened peptide P756			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCGCTTGGACCTCCGGTCTATCTATAAGCCCCATAACTTCGATATTTACTTTATTTATTAA	NRLDLRSIYKPHNFDIYFIY*	-2.398	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22407	TGTLPCNCQAIAGINLVVSI			20	SLAY-screened peptide P757			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGGTACTCTCCCTTGCAATTGCCAGGCTATTGCTGGTATTAATCTCGTGGTTTCCATCTAA	TGTLPCNCQAIAGINLVVSI*	-2.397	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22408	RRHRLFSLYHTAPSLCSYDP			20	SLAY-screened peptide P758			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGTCACCGTCTCTTTAGCCTTTACCACACCGCTCCTTCGCTGTGCTCTTATGATCCGTAA	RRHRLFSLYHTAPSLCSYDP*	-2.396	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22409	SQGTTYNLLMLSHVNSHS			18	SLAY-screened peptide P759			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCAGGGGACCACCTATAACCTCTTGATGTTGTCGCATGTGAATTCTCACAGCTAGTGCTAA	SQGTTYNLLMLSHVNSHS*C*	-2.392	1.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22410	TCPIILVYNLSTCNMTGSHR			20	SLAY-screened peptide P760			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTGTCCTATTATTCTCGTTTACAACTTGTCGACGTGCAACATGACTGGGTCTCATCGGTAA	TCPIILVYNLSTCNMTGSHR*	-2.389	2.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22411	LEDSGAVFSGSCHIPPMSLV			20	SLAY-screened peptide P761			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGAGGATAGTGGGGCGGTGTTCAGTGGCTCCTGCCATATTCCTCCGATGTCTCTTGTGTAA	LEDSGAVFSGSCHIPPMSLV*	-2.388	0.001076	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22412	QFYT			4	SLAY-screened peptide P762			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTTTTATACCTAGCTTGCTAATGGGTAGCTTAAGGCTAACATTGAGACCATTCACGAGTAA	QFYT*LANG*LKANIETIHE*	-2.386	7.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22413	GCTPVTFHNVPYLITNNGFH			20	SLAY-screened peptide P763			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTGCACTCCTGTCACGTTTCATAATGTTCCTTATCTCATCACCAATAATGGGTTTCATTAA	GCTPVTFHNVPYLITNNGFH*	-2.386	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22414	CLQPYDIS			8	SLAY-screened peptide P764			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCTGCAGCCTTACGACATTTCTTAGTATATTGCTGACGTGTCCATGAGGATGGTTGTTTAA	CLQPYDIS*YIADVSMRMVV*	-2.383	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22415	PHLHPRPPRRSVPHCLLMWF			20	SLAY-screened peptide P765			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCACCTGCATCCGCGGCCCCCCCGCCGGTCTGTGCCCCACTGTCTGCTGATGTGGTTCTAA	PHLHPRPPRRSVPHCLLMWF*	-2.382	0.000489	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22416	PCPALLIISPLSLQPSLLIF			20	SLAY-screened peptide P766			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGCCCCGCCCTGCTCATCATCAGTCCTTTGTCCCTGCAGCCTTCCCTTCTGATCTTTTAA	PCPALLIISPLSLQPSLLIF*	-2.382	0.000154	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22417	RMLSCTPRLPSRRPYPGPGPN			21	SLAY-screened peptide P767			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATGCTTTCGTGTACCCCCAGGCTGCCCTCTCGTCGCCCTTACCCTGGGCCCGGTCCTAAC	RMLSCTPRLPSRRPYPGPGPN	-2.381	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22418	GIDFYNHVYIFVEPSPSPLCN			21	SLAY-screened peptide P768			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCATCGACTTTTATAATCACGTGTACATTTTCGTGGAGCCTAGCCCTTCTCCGCTATGTAAC	GIDFYNHVYIFVEPSPSPLCN	-2.38	2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22419	CYNSFLRKRRKFKVYLTCLS			20	SLAY-screened peptide P769			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTACAATTCCTTCCTTCGTAAGCGTCGCAAGTTTAAGGTTTACCTCACCTGTCTCTCCTAA	CYNSFLRKRRKFKVYLTCLS*	-2.38	9.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22420	TSPHRHLNYPPLPVLNT			17	SLAY-screened peptide P770			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTCTCCTCACAGGCACCTTAACTACCCTCCGTTGCCGGTGCTTAACACTTAGACTCGGTAA	TSPHRHLNYPPLPVLNT*TR*	-2.378	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22421	ASDNHRRLGVTITFSVYFNF			20	SLAY-screened peptide P771			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTCTGACAACCATCGCCGTCTGGGTGTTACGATCACGTTTTCTGTTTACTTTAACTTTTAA	ASDNHRRLGVTITFSVYFNF*	-2.378	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22422	TSYGSIPYVLALGSRNPNA			19	SLAY-screened peptide P772			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAGTTACGGTAGCATTCCCTACGTTCTGGCTCTTGGCTCTCGCAATCCCAACGCCTAACTG	TSYGSIPYVLALGSRNPNA*L	-2.378	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22423	AMAHCGKPIVPHHRLCY			17	SLAY-screened peptide P773			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCATGGCTCACTGCGGCAAGCCTATTGTTCCGCATCACCGTCTCTGTTACTAGGACACTTAA	AMAHCGKPIVPHHRLCY*DT*	-2.378	0.000287	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22424	SGCRRESFSRLRDRFYSNRV			20	SLAY-screened peptide P774			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGGGTGCCGCAGGGAGTCGTTCAGTAGGCTGCGTGATCGCTTTTACAGTAACCGTGTTTAA	SGCRRESFSRLRDRFYSNRV*	-2.378	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22425	GNDRRYTNVTTDIRSNFIML			20	SLAY-screened peptide P775			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTAATGACCGTCGCTACACCAACGTCACCACGGATATTAGGTCTAATTTTATTATGTTGTAA	GNDRRYTNVTTDIRSNFIML*	-2.378	0.00195	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22426	LAAPK			5	SLAY-screened peptide P776			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGGCTGCTCCGAAGTAGGCGCATGGGTACTCTGACTATTGCCCCACCCAGATCGTCTCGTAA	LAAPK*AHGYSDYCPTQIVS*	-2.377	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22427	APWRPRGRAWRSIFTYRRKA			20	SLAY-screened peptide P777			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCCTTGGCGGCCCCGGGGCCGCGCGTGGCGGAGTATCTTTACGTATCGTCGCAAGGCCTAA	APWRPRGRAWRSIFTYRRKA*	-2.376	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22428	KSSFLDGRWYYSNLHNRGLA			20	SLAY-screened peptide P778			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGAGCTCCTTTCTCGACGGTCGGTGGTATTATAGCAACCTGCATAATCGTGGTCTTGCGTAA	KSSFLDGRWYYSNLHNRGLA*	-2.376	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22429	LSPMSLLHTGVMRCNVNADF			20	SLAY-screened peptide P779			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCCCCTATGTCCCTCCTGCACACTGGCGTTATGCGTTGTAACGTTAACGCTGACTTCTAA	LSPMSLLHTGVMRCNVNADF*	-2.375	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22430	AFTPSVKNPSPANGAPNDCI			20	SLAY-screened peptide P780			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTTTACCCCTTCCGTTAAGAACCCTTCTCCCGCCAATGGTGCCCCCAATGACTGCATTTAA	AFTPSVKNPSPANGAPNDCI*	-2.375	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22431	IPEILLSLYRRYLNVYKWVH			20	SLAY-screened peptide P781			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCCGGAGATCCTCCTCTCTCTTTACCGCCGTTACTTGAACGTTTACAAGTGGGTCCATTAA	IPEILLSLYRRYLNVYKWVH*	-2.374	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22432	PVVHLNLLRYLWSIYSNAVL			20	SLAY-screened peptide P782			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGTGGTGCACCTCAACTTGCTGAGGTATCTGTGGAGCATTTACAGTAATGCGGTTCTTTAA	PVVHLNLLRYLWSIYSNAVL*	-2.373	0.000335	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22433	SLGIYDGRIGGTNLNREKAY			20	SLAY-screened peptide P783			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTTGGGCATCTATGATGGTCGCATTGGCGGGACTAATCTTAATCGGGAGAAGGCTTACTAA	SLGIYDGRIGGTNLNREKAY*	-2.373	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22434	VTIVQPIPLSPPDSATCMHQ			20	SLAY-screened peptide P784			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTACTATTGTGCAGCCGATTCCCCTGTCCCCTCCTGACTCGGCCACTTGCATGCATCAGTAA	VTIVQPIPLSPPDSATCMHQ*	-2.372	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22435	GSTWYYNSTIRANYIIDNTR			20	SLAY-screened peptide P785			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGAGCACGTGGTATTATAATAGCACTATTAGGGCCAATTATATTATTGATAATACTAGGTAA	GSTWYYNSTIRANYIIDNTR*	-2.372	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22436	RDKFYLRGNPNGMNMTVTIC			20	SLAY-screened peptide P786			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGATAAGTTTTATCTGCGTGGTAATCCCAATGGGATGAACATGACGGTTACTATTTGTTAA	RDKFYLRGNPNGMNMTVTIC*	-2.371	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22437	TGDPYCHSLPITPTHNMCDL			20	SLAY-screened peptide P787			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGGTGATCCCTACTGTCATTCCCTGCCTATTACTCCGACCCATAATATGTGTGATCTTTAA	TGDPYCHSLPITPTHNMCDL*	-2.37	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22438	VCYCSNRSEWPSHIYVHETH			20	SLAY-screened peptide P788			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTGCTATTGTTCTAATCGCAGTGAGTGGCCTTCCCACATTTATGTTCACGAGACTCACTAA	VCYCSNRSEWPSHIYVHETH*	-2.37	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22439	CHRAPPISYIHYSFHLYS			18	SLAY-screened peptide P789			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCATCGTGCTCCCCCTATTTCCTATATTCACTATTCTTTTCACCTGTACTCCTAGAGTTAA	CHRAPPISYIHYSFHLYS*S*	-2.368	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22440	LNFSCARTNSLAPLILRT			18	SLAY-screened peptide P790			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAATTTTTCCTGTGCCCGTACCAACTCGCTTGCTCCTTTGATTCTGCGCACTTAGTTGTAA	LNFSCARTNSLAPLILRT*L*	-2.367	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22441	DYFHYLTFLFSLAIIFTTSLN			21	SLAY-screened peptide P791			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTACTTTCACTATCTGACGTTCTTGTTCTCATTGGCAATTATATTCACAACCTCACTTAAC	DYFHYLTFLFSLAIIFTTSLN	-2.367	8.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22442	PPTLMCPSITLAFTRRRSC			19	SLAY-screened peptide P792			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCACTCTTATGTGTCCCTCGATTACTTTGGCGTTCACCCGGCGCCGGTCCTGTTAGTAA	PPTLMCPSITLAFTRRRSC**	-2.364	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22443	TLIHIVIYGPMVLLGLCTSKS			21	SLAY-screened peptide P793			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTTATCCACATTGTGATCTATGGCCCCATGGTCCTGTTGGGCCTTTGTACGAGTAAGTCG	TLIHIVIYGPMVLLGLCTSKS	-2.364	0.000356	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22444	TVVLPLSVAIVYSAYSAPHL			20	SLAY-screened peptide P794			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTCGTCCTGCCCCTGTCTGTTGCCATTGTTTATTCCGCTTACTCGGCCCCTCACCTCTAA	TVVLPLSVAIVYSAYSAPHL*	-2.363	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22445	QRRVLVAGNSSCAIQTLRCA			20	SLAY-screened peptide P795			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCGGCGTGTTCTGGTGGCCGGTAATTCCAGCTGCGCGATCCAGACGCTGCGTTGTGCGTAA	QRRVLVAGNSSCAIQTLRCA*	-2.363	0.000212	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22446	APVAHNPRSGRNPAKTPTNT			20	SLAY-screened peptide P796			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCGGTTGCGCACAATCCGCGTTCGGGTCGTAACCCGGCCAAGACGCCCACCAACACTTAA	APVAHNPRSGRNPAKTPTNT*	-2.362	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22447	HLGITVDNCTAYAIDVLPNT			20	SLAY-screened peptide P797			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTTGGGTATTACTGTTGACAATTGCACGGCGTACGCCATCGATGTGCTTCCTAACACCTAA	HLGITVDNCTAYAIDVLPNT*	-2.361	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22448	TLTA			4	SLAY-screened peptide P798			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTTACGGCTTAGGTGCTCACTTAGAGTCAGAACATTCTCCTCACTTATTAGGCGTGCTAA	TLTA*VLT*SQNILLTY*AC*	-2.361	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22449	DPNVSTFSHCRHRLAFFSDSH			21	SLAY-screened peptide P799			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCCTAATGTTTCGACTTTCTCCCACTGCCGCCACCGACTGGCATTCTTCTCAGATTCACAT	DPNVSTFSHCRHRLAFFSDSH	-2.36	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22450	SSTKRPDSDDHDVSLQDNTH			20	SLAY-screened peptide P800			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCAGTACTAAGCGCCCTGACTCTGATGATCATGACGTGTCTCTCCAGGATAACACTCACTAA	SSTKRPDSDDHDVSLQDNTH*	-2.36	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22451	AGVTIILLPGAATFVIRRRRN			21	SLAY-screened peptide P801			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGGAGTTACCATCATCTTACTGCCTGGCGCCGCGACGTTTGTTATAAGACGACGCCGTAAC	AGVTIILLPGAATFVIRRRRN	-2.36	0.000311	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22452	PRWGPAWNIFRLFYKSRHPS			20	SLAY-screened peptide P802			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGCTGGGGCCCCGCGTGGAACATCTTCCGGCTCTTCTATAAGTCTCGTCACCCCTCGTAA	PRWGPAWNIFRLFYKSRHPS*	-2.359	1.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22453	HLHMATYINNHYSTYFFTFF			20	SLAY-screened peptide P803			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCTTCATATGGCTACGTATATCAACAATCATTACAGTACCTATTTTTTTACCTTCTTCTAA	HLHMATYINNHYSTYFFTFF*	-2.358	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22454	NYSGR			5	SLAY-screened peptide P804			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTACAGCGGCCGCTAGGCTGCTGCTTTTCTTGACACCTGTGCCACTTATCCGATTGCGTAA	NYSGR*AAAFLDTCATYPIA*	-2.357	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22455	PPTHLSTRMCVSSATHADSR			20	SLAY-screened peptide P805			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCGACTCATCTTAGTACTCGTATGTGCGTGTCGAGCGCCACCCACGCTGATTCGAGGTAA	PPTHLSTRMCVSSATHADSR*	-2.355	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22456	NFYKPEFLQDYLTSYYYVLA			20	SLAY-screened peptide P806			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTTCTACAAGCCTGAGTTTCTCCAGGACTATCTTACGAGTTATTATTATGTCCTTGCCTAA	NFYKPEFLQDYLTSYYYVLA*	-2.355	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22457	YADTSGPALDHSNCSVCGCM			20	SLAY-screened peptide P807			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGCCGACACCTCCGGCCCCGCCTTGGACCACAGCAACTGTAGTGTTTGCGGCTGCATGTAA	YADTSGPALDHSNCSVCGCM*	-2.354	2.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22458	VPPDTIVRDDSVALLTTLRH			20	SLAY-screened peptide P808			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCCGCCTGATACGATTGTCCGTGATGATTCGGTGGCCCTGCTTACTACCCTTAGGCACTAA	VPPDTIVRDDSVALLTTLRH*	-2.353	2.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22459	PRKLILILKSYRQKKTRKNS			20	SLAY-screened peptide P809			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGCAAGCTGATTCTCATCCTTAAGAGTTACCGCCAGAAGAAGACTAGGAAGAATAGCTAA	PRKLILILKSYRQKKTRKNS*	-2.353	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22460	PPDNRDTSWPAQERGPADHY			20	SLAY-screened peptide P810			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCGATAACCGGGATACCTCTTGGCCTGCTCAGGAGAGGGGTCCGGCGGACCATTATTAA	PPDNRDTSWPAQERGPADHY*	-2.351	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22461	FQSAESSPSVAIHIVDDLIH			20	SLAY-screened peptide P811			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCAGTCTGCGGAGTCGAGTCCTAGCGTGGCTATCCATATCGTCGATGATCTGATTCATTAA	FQSAESSPSVAIHIVDDLIH*	-2.35	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22462	YAHAPYEDDVPPQHGVVTE			19	SLAY-screened peptide P812			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGCTCACGCCCCATATGAGGACGACGTGCCGCCACAACACGGAGTGGTAACTGAGTAAGTC	YAHAPYEDDVPPQHGVVTE*V	-2.349	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22463	VDREFLLDYKSYAKKYSLIV			20	SLAY-screened peptide P813			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGACCGTGAGTTTCTGCTTGACTACAAGTCCTACGCGAAGAAGTACTCGCTGATTGTTTAA	VDREFLLDYKSYAKKYSLIV*	-2.348	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22464	WKNQHGYPSHCSNMEFGNHDN			21	SLAY-screened peptide P814			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGAAGAATCAGCACGGCTACCCCTCCCATTGCAGTAATATGGAGTTTGGCAATCATGATAAC	WKNQHGYPSHCSNMEFGNHDN	-2.348	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22465	PICPHMHRDWIMFNNTSWIS			20	SLAY-screened peptide P815			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATTTGCCCCCACATGCATAGGGATTGGATCATGTTCAATAATACCTCTTGGATTAGCTAA	PICPHMHRDWIMFNNTSWIS*	-2.348	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22466	RGHHDSYSVRCYLSPDPDHP			20	SLAY-screened peptide P816			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGGTCACCACGATTCTTATTCCGTTCGTTGTTACCTCTCTCCCGATCCTGACCACCCGTAA	RGHHDSYSVRCYLSPDPDHP*	-2.347	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22467	FYQVPLQNHRLDMHRPHNNN			20	SLAY-screened peptide P817			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTACCAGGTTCCTCTGCAGAACCACCGTCTGGACATGCATCGTCCGCATAATAATAATTAA	FYQVPLQNHRLDMHRPHNNN*	-2.346	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22468	WHS			3	SLAY-screened peptide P818			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCACTCCTAGAAGACCTATCCCAGTTTGTCGATCCTTCTGCTCTAGACCGCGACGTTCTAA	WHS*KTYPSLSILLL*TATF*	-2.346	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22469	YRVQPDDNVLQVRVTVAIIFN			21	SLAY-screened peptide P819			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCGTGTGCAGCCCGATGACAACGTCCTGCAGGTTAGGGTTACAGTTGCGATTATATTTAAC	YRVQPDDNVLQVRVTVAIIFN	-2.345	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22470	RWFAAHDFGHDCNKPIPTDT			20	SLAY-screened peptide P820			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTGGTTCGCGGCGCACGACTTTGGTCATGATTGTAACAAGCCGATTCCTACTGATACTTAA	RWFAAHDFGHDCNKPIPTDT*	-2.344	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22471	RRSYPLLLYHFNFKCS			16	SLAY-screened peptide P821			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCGCAGTTACCCTCTTTTGCTTTATCACTTTAACTTTAAGTGCTCTTAGGGGACCGACTAA	RRSYPLLLYHFNFKCS*GTD*	-2.344	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22472	LRRQPAAYMLYRTHMFLI			18	SLAY-screened peptide P822			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAGGAGGCAGCCCGCTGCGTATATGCTCTACCGTACGCATATGTTTCTCATTTAGAACTAA	LRRQPAAYMLYRTHMFLI*N*	-2.344	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22473	DRGTRHTSPDSYAFTSAINT			20	SLAY-screened peptide P823			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCGTGGCACGCGCCATACGTCGCCCGACTCGTATGCCTTTACTTCTGCCATTAACACCTAA	DRGTRHTSPDSYAFTSAINT*	-2.343	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22474	LNPCTSAVIPFDYIYIQFYE			20	SLAY-screened peptide P824			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAACCCGTGTACGAGTGCTGTCATTCCTTTCGATTACATTTATATTCAGTTTTACGAGTAA	LNPCTSAVIPFDYIYIQFYE*	-2.342	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22475	CCMSLVLVSSKCLCFIV			17	SLAY-screened peptide P825			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTGCATGTCTCTGGTTTTGGTTTCTTCTAAGTGCCTCTGTTTCATCGTGTAGGCTTCCTAA	CCMSLVLVSSKCLCFIV*AS*	-2.341	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22476	SSYFLERPRD			10	SLAY-screened peptide P826			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTCTTATTTTTTGGAGCGTCCTCGTGACTAGTCGGACTACGTGAATATTATGCACATGTAA	SSYFLERPRD*SDYVNIMHM*	-2.34	0.000343	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22477	YVSPQGIPLRSWRFFNIVPV			20	SLAY-screened peptide P827			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGTTAGCCCTCAGGGTATTCCCCTTAGGAGCTGGCGCTTTTTTAACATTGTTCCCGTGTAA	YVSPQGIPLRSWRFFNIVPV*	-2.339	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22478	RAANLPVGPHLIKRSAPYLH			20	SLAY-screened peptide P828			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGCCGCCAACCTTCCCGTGGGCCCCCACCTCATTAAGCGTAGCGCGCCCTACTTGCATTAA	RAANLPVGPHLIKRSAPYLH*	-2.338	0.000955	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22479	FNSEHQVSRGSVVVIFVLDN			20	SLAY-screened peptide P829			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAATTCCGAGCATCAGGTGTCTCGGGGTAGCGTGGTGGTTATTTTCGTTCTGGACAACTAA	FNSEHQVSRGSVVVIFVLDN*	-2.336	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22480	PPCPVLLAFPLALARVSPIN			20	SLAY-screened peptide P830			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCGTGCCCTGTACTTCTGGCATTCCCTCTCGCACTAGCGCGGGTATCACCCATTAACTGA	PPCPVLLAFPLALARVSPIN*	-2.335	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22481	SSCRVLSSD			9	SLAY-screened peptide P831			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTCCTGCCGTGTCCTTTCCTCTGACTAGTTTCGCTCCTGCCCCGAGTGCCAGTCCTTCTAA	SSCRVLSSD*FRSCPECQSF*	-2.334	0.000119	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22482	ITWALFLWYGMPNFDIDRSE			20	SLAY-screened peptide P832			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCACCTGGGCCCTTTTCCTCTGGTACGGTATGCCCAATTTTGACATTGATAGGTCGGAGTAA	ITWALFLWYGMPNFDIDRSE*	-2.333	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22483	IDIPDYDYQSLC			12	SLAY-screened peptide P833			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGACATTCCCGATTATGATTATCAGAGCCTTTGTTAGCTTTACCCCACTATTTATTGGTAA	IDIPDYDYQSLC*LYPTIYW*	-2.332	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22484	YSHMRRSHHDHVRPSDFLSS			20	SLAY-screened peptide P834			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACAGCCACATGCGTCGTTCTCATCATGATCACGTGCGGCCCAGCGATTTCCTGTCTTCTTAA	YSHMRRSHHDHVRPSDFLSS*	-2.332	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22485	HYPAHSYSVQIMWRNIANLP			20	SLAY-screened peptide P835			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTACCCTGCCCATTCGTACAGTGTTCAGATTATGTGGCGCAATATTGCGAATCTTCCGTAA	HYPAHSYSVQIMWRNIANLP*	-2.332	2.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22486	VPNSTATLNAYW			12	SLAY-screened peptide P836			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCCCAATTCTACCGCTACGCTCAACGCTTATTGGTAGTGCAGGAATGGCCTTAACTCCTAA	VPNSTATLNAYW*CRNGLNS*	-2.331	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22487	EDYTFLLVFVHCFVRFKSKST			21	SLAY-screened peptide P837			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGACTATACTTTTCTCCTTGTTTTCGTCCATTGCTTCGTGCGCTTCAAGAGTAAGTCGACC	EDYTFLLVFVHCFVRFKSKST	-2.33	0.000361	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22488	RNGFNREYCSSHVCIFNSVNN			21	SLAY-screened peptide P838			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGAATGGCTTTAACCGTGAGTATTGCTCTTCGCACGTTTGTATCTTTAATAGTGTGAATAAC	RNGFNREYCSSHVCIFNSVNN	-2.328	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22489	YLDVSSNIYDREYLLLCTCS			20	SLAY-screened peptide P839			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCTTGACGTTTCCAGTAATATTTATGACCGGGAGTACCTCTTGCTTTGCACGTGTTCGTAA	YLDVSSNIYDREYLLLCTCS*	-2.327	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22490	SHV			3	SLAY-screened peptide P840			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCATGTCTAGAGCCATTGTCAGTATTATGTGGTCCTCACGCTTGATAGCCTTACTGTGTAA	SHV*SHCQYYVVLTLDSLTV*	-2.327	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22491	NLEMSALPLPLYLVRLLAVN			20	SLAY-screened peptide P841			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTTGGAGATGTCTGCGTTGCCTCTGCCTCTCTACTTGGTCCGTTTGTTGGCCGTGAATTAA	NLEMSALPLPLYLVRLLAVN*	-2.327	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22492	PPSRPSLHNSSWFPLMYECL			20	SLAY-screened peptide P842			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCGTCTCGCCCTTCTCTGCATAATTCTTCGTGGTTCCCGCTTATGTACGAGTGCCTTTAA	PPSRPSLHNSSWFPLMYECL*	-2.327	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22493	TTFNLYARHQARLNTHENAP			20	SLAY-screened peptide P843			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACTTTTAATCTGTATGCCCGTCATCAGGCTCGGCTTAACACTCATGAGAATGCTCCTTAA	TTFNLYARHQARLNTHENAP*	-2.326	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22494	IRRCAFAFNVLAQNQNKMTT			20	SLAY-screened peptide P844			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAGGCGTTGCGCGTTCGCTTTTAACGTGTTGGCGCAGAATCAGAACAAGATGACCACTTAA	IRRCAFAFNVLAQNQNKMTT*	-2.326	7.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22495	HYPNSPLPRFLNIILHRCLIN			21	SLAY-screened peptide P845			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTATCCTAACTCGCCTCTCCCCCGTTTTCTCAACATTATTCTCCATAGGTGCTTGATTAAC	HYPNSPLPRFLNIILHRCLIN	-2.325	0.000984	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22496	HITRCAPDWAYVLLHPH			17	SLAY-screened peptide P846			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATATCACCCGCTGCGCCCCGGACTGGGCTTACGTCCTGCTTCATCCTCATTAGGGGAGCTAA	HITRCAPDWAYVLLHPH*GS*	-2.325	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22497	ELTPALSRYVALIETYNSAP			20	SLAY-screened peptide P847			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCTCACCCCGGCGCTCTCGCGTTATGTCGCTCTCATCGAGACGTATAACTCGGCCCCGTAA	ELTPALSRYVALIETYNSAP*	-2.324	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22498	HAANSACRHPMQIPVPDIIY			20	SLAY-screened peptide P848			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGCTGCTAACTCTGCTTGTCGCCACCCTATGCAGATTCCTGTCCCCGACATTATCTACTAA	HAANSACRHPMQIPVPDIIY*	-2.324	0.000669	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22499	RSGAHLLLTHRRGRACGLSV			20	SLAY-screened peptide P849			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCCGGCGCGCATCTTCTCCTCACTCACCGCCGTGGGCGCGCTTGTGGCCTGTCCGTGTAA	RSGAHLLLTHRRGRACGLSV*	-2.323	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22500	YQFINMCSVPFHPPC			15	SLAY-screened peptide P850			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCAGTTTATTAACATGTGCTCTGTGCCTTTTCATCCTCCTTGCTAGAGCACCAGCGGTTAA	YQFINMCSVPFHPPC*STSG*	-2.323	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22501	LHSPHSAPHFARPASRNIDT			20	SLAY-screened peptide P851			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCATAGTCCGCATTCCGCTCCCCATTTTGCTAGGCCTGCTAGCAGGAACATTGATACGTAA	LHSPHSAPHFARPASRNIDT*	-2.322	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22502	CQKMAISWLQIVAILLVSTF			20	SLAY-screened peptide P852			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCAGAAGATGGCGATTAGTTGGCTTCAGATTGTCGCCATTTTGCTGGTCTCTACTTTCTAA	CQKMAISWLQIVAILLVSTF*	-2.322	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22503	WNQKRLRHRHTPSCSDENYL			20	SLAY-screened peptide P853			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGAACCAGAAGCGTCTCCGTCATCGTCATACTCCGTCTTGCTCCGACGAGAATTATCTGTAA	WNQKRLRHRHTPSCSDENYL*	-2.319	0.000663	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22504	GGEPAPLSDCACPSCAVCDR			20	SLAY-screened peptide P854			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGGTGAGCCTGCGCCGCTCTCGGATTGCGCCTGTCCTTCTTGTGCGGTGTGTGATCGGTAA	GGEPAPLSDCACPSCAVCDR*	-2.319	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22505	NFLLFDRNCHYPLTFGPVLG			20	SLAY-screened peptide P855			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTTCCTTTTGTTTGATAGGAATTGTCATTATCCCTTGACCTTTGGTCCGGTTCTTGGTTAA	NFLLFDRNCHYPLTFGPVLG*	-2.319	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22506	NTYTFMLYGLGHTLLAPRAGN			21	SLAY-screened peptide P856			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATACCTACACCTTCATGCTTTACGGCCTGGGTCACACTCTGCTGGCGCCCCGCGCCGGTAAC	NTYTFMLYGLGHTLLAPRAGN	-2.319	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22507	PPLDTVTHAICEIKYFRQSL			20	SLAY-screened peptide P857			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCCTTGGACACTGTCACGCACGCCATCTGCGAGATCAAGTACTTTCGCCAGTCGCTCTAA	PPLDTVTHAICEIKYFRQSL*	-2.318	0.001013	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22508	GPPVLSFVAINVDAPITTTTN			21	SLAY-screened peptide P858			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCCCCCTGTCCTGTCCTTTGTGGCCATTAATGTTGACGCCCCTATCACTACAACTACTAAC	GPPVLSFVAINVDAPITTTTN	-2.317	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22509	VTTFTNYYNRAVTLIHLT			18	SLAY-screened peptide P859			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTACGACCTTTACGAATTATTATAATCGTGCCGTCACTCTGATTCACCTGACTTAGCTTTAA	VTTFTNYYNRAVTLIHLT*L*	-2.316	8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22510	YLRYRTDPNYFHSYNSFCSD			20	SLAY-screened peptide P860			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCTTAGGTATCGCACTGATCCTAATTATTTCCATAGTTATAATAGCTTTTGCAGTGACTAA	YLRYRTDPNYFHSYNSFCSD*	-2.316	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22511	SSEKCYATFLAARYHIFN			18	SLAY-screened peptide P861			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTCCGAGAAGTGTTATGCTACTTTTCTGGCTGCGCGCTATCATATTTTTAATTAGAACTAA	SSEKCYATFLAARYHIFN*N*	-2.315	0.000645	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22512	VATFVLCLYRTNIQSFLHWD			20	SLAY-screened peptide P862			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGCCACTTTTGTTCTTTGCCTCTATCGCACGAACATTCAGAGCTTTCTTCACTGGGATTAA	VATFVLCLYRTNIQSFLHWD*	-2.315	2.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22513	RCYYPGHIIFKGYVMYNNST			20	SLAY-screened peptide P863			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGTTATTACCCGGGGCATATTATCTTTAAGGGGTACGTCATGTATAATAATTCCACGTAA	RCYYPGHIIFKGYVMYNNST*	-2.314	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22514	APSRCAFHGT			10	SLAY-screened peptide P864			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCCGTCTAGGTGTGCTTTCCACGGTACTTAGGACCATAGTTTTCATAGTTCTTGCACTTAA	APSRCAFHGT*DHSFHSSCT*	-2.313	0.000646	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22515	LHFLVNTYCTQHVVDLRNMH			20	SLAY-screened peptide P865			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCACTTCCTTGTTAACACCTATTGCACTCAGCACGTCGTTGATTTGCGTAATATGCACTAA	LHFLVNTYCTQHVVDLRNMH*	-2.312	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22516	VYGILLLLRRRPWCRPLLTCN			21	SLAY-screened peptide P866			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTACGGTATACTCCTCTTATTAAGAAGAAGGCCATGGTGCCGACCCTTACTAACCTGTAAC	VYGILLLLRRRPWCRPLLTCN	-2.312	0.001166	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22517	IAIPTSYTNVTPRAHSMASL			20	SLAY-screened peptide P867			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGCTATCCCCACCAGCTACACGAACGTTACGCCTCGCGCTCACAGCATGGCTTCGCTTTAA	IAIPTSYTNVTPRAHSMASL*	-2.312	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22518	PMSSTVRLRTTNDDINSCTN			20	SLAY-screened peptide P868			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATGTCTAGTACGGTCCGCCTCCGGACTACCAATGATGACATTAATTCGTGTACTAATTAA	PMSSTVRLRTTNDDINSCTN*	-2.311	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22519	INTKHGGYRLVCNNLSVHSG			20	SLAY-screened peptide P869			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAACACCAAGCACGGTGGCTATCGCCTGGTCTGCAACAATCTGTCTGTCCATTCCGGTTAA	INTKHGGYRLVCNNLSVHSG*	-2.311	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22520	LRCAIFWPIILIPP			14	SLAY-screened peptide P870			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCGGTGCGCCATCTTCTGGCCTATCATTCTCATTCCTCCTTAGGTCTGTGATAAGGCCTAA	LRCAIFWPIILIPP*VCDKA*	-2.311	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22521	NTNAMPVSWCSHGCSCGMD			19	SLAY-screened peptide P871			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACACGAACGCCATGCCTGTTAGCTGGTGTTCTCACGGTTGCAGTTGCGGCATGGATTAGTAA	NTNAMPVSWCSHGCSCGMD**	-2.31	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22522	AQADHSNVV			9	SLAY-screened peptide P872			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCAGGCGGATCATTCTAACGTTGTTTAGAATTGCTGTGATAGTCTTGTTTAGTCGTATTAA	AQADHSNVV*NCCDSLV*SY*	-2.308	0.001745	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22523	DVWSRWCYPLTFANADLLPI			20	SLAY-screened peptide P873			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGTTTGGTCCCGCTGGTGCTATCCTTTGACGTTTGCTAATGCCGACTTGCTCCCTATTTAA	DVWSRWCYPLTFANADLLPI*	-2.308	0.002886	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22524	SFILHSIAVSPGISAPDFRY			20	SLAY-screened peptide P874			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTTTATCCTCCATTCGATCGCCGTGTCCCCTGGCATCAGCGCTCCGGACTTTCGTTATTAA	SFILHSIAVSPGISAPDFRY*	-2.307	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22525	YAGPMHIYHNYHCRYSTCSL			20	SLAY-screened peptide P875			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGCGGGCCCGATGCACATTTACCATAACTATCACTGCCGGTATTCGACCTGCAGCCTTTAA	YAGPMHIYHNYHCRYSTCSL*	-2.307	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22526	SHRSHENIPMGIVDSDIIDI			20	SLAY-screened peptide P876			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCATCGGTCCCATGAGAATATTCCGATGGGTATTGTTGACTCCGACATCATTGATATTTAA	SHRSHENIPMGIVDSDIIDI*	-2.305	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22527	PTPAIYHLRYNRPGEMQPSA			20	SLAY-screened peptide P877			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACGCCTGCTATTTACCACCTTAGGTATAACCGTCCTGGTGAGATGCAGCCCTCGGCCTAA	PTPAIYHLRYNRPGEMQPSA*	-2.305	0.000228	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22528	RTSWGPCTNNRSAND			15	SLAY-screened peptide P878			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACTAGCTGGGGCCCTTGTACTAACAATCGCTCTGCGAATGACTAGTATTATCCTAATTAA	RTSWGPCTNNRSAND*YYPN*	-2.304	6.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22529	NHHAPCISVL			10	SLAY-screened peptide P879			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCACCATGCGCCCTGCATCTCTGTCCTGTAGCGGATGGCTCTTACCATGGACGGTGCGTAA	NHHAPCISVL*RMALTMDGA*	-2.303	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22530	LHDNVTDPLFCSRTPSTKAA			20	SLAY-screened peptide P880			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCACGATAACGTGACTGACCCGCTCTTTTGTAGCCGTACCCCGAGTACGAAGGCTGCCTAA	LHDNVTDPLFCSRTPSTKAA*	-2.303	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22531	SMVPRRHYDPGSKSLTSFWS			20	SLAY-screened peptide P881			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTATGGTCCCTCGTAGGCACTACGACCCGGGCTCTAAGTCCCTTACCAGTTTCTGGAGTTAA	SMVPRRHYDPGSKSLTSFWS*	-2.303	0.000146	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22532	TMTTHRLSLCHRNCHRRHLSR			21	SLAY-screened peptide P882			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTATGACGACTCATCGTCTCTCTTTGTGCCACCGCAATTGCCACCGGCGTCATTTAAGTCGA	TMTTHRLSLCHRNCHRRHLSR	-2.303	2.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22533	ACQSASLYQIHRDEALTLKN			20	SLAY-screened peptide P883			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTGTCAGTCGGCCAGTCTGTACCAGATCCATAGGGACGAGGCTCTCACCCTCAAGAATTAA	ACQSASLYQIHRDEALTLKN*	-2.302	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22534	MHPRHKQYWFHLLLLHIANT			20	SLAY-screened peptide P884			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCACCCCAGGCACAAGCAGTACTGGTTTCATCTTTTGCTCTTGCATATTGCTAATACTTAA	MHPRHKQYWFHLLLLHIANT*	-2.302	2.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22535	TLHRYHYRQINMVSILSSHV			20	SLAY-screened peptide P885			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCTCCATCGTTATCACTACCGTCAGATTAACATGGTCTCTATTCTGAGTTCTCATGTGTAA	TLHRYHYRQINMVSILSSHV*	-2.3	0.000131	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22536	PQAEISYSLCILRAR			15	SLAY-screened peptide P886			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCAGGCGGAGATCTCCTATAGCCTTTGCATTCTTCGGGCTCGTTAGTGGTTGTTCAATTAA	PQAEISYSLCILRAR*WLFN*	-2.299	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22537	VCTYAHPYRRYWIPYVYMRL			20	SLAY-screened peptide P887			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTGTACTTACGCTCACCCCTATCGGCGCTACTGGATTCCTTATGTCTATATGCGCTTGTAA	VCTYAHPYRRYWIPYVYMRL*	-2.296	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22538	RRSAVRVRHPPGISTYYRLP			20	SLAY-screened peptide P888			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCGCAGCGCTGTTAGGGTTCGGCATCCTCCGGGGATTAGCACGTATTATCGGCTTCCCTAA	RRSAVRVRHPPGISTYYRLP*	-2.295	5.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22539	PQINFIPLPLLLVSRVFPWVN			21	SLAY-screened peptide P889			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCAGATTAACTTCATTCCGTTACCCCTACTGTTGGTTAGCCGTGTTTTCCCCTGGGTTAAC	PQINFIPLPLLLVSRVFPWVN	-2.295	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22540	AVGLPSYMFHPVMPNLRNHN			20	SLAY-screened peptide P890			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGTGGGGCTTCCCTCTTACATGTTCCATCCCGTTATGCCCAATCTTAGGAATCACAACTAA	AVGLPSYMFHPVMPNLRNHN*	-2.294	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22541	HDQRAVVTSLSFLLLVLSSV			20	SLAY-screened peptide P891			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGATCAGCGCGCGGTTGTCACTAGTCTTAGCTTCCTGTTGCTCGTTCTGTCCTCCGTTTAA	HDQRAVVTSLSFLLLVLSSV*	-2.293	0.000637	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22542	FRPLQAEAYNY			11	SLAY-screened peptide P892			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCGGCCCTTGCAGGCTGAGGCTTACAACTATTAGAACGTTGCGTGCATTTTTAGTCAGTAA	FRPLQAEAYNY*NVACIFSQ*	-2.291	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22543	PFRSNTNKYTMCSGESFSNS			20	SLAY-screened peptide P893			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTCCGCAGTAATACGAATAAGTATACTATGTGTTCCGGCGAGAGCTTTTCGAATAGTTAA	PFRSNTNKYTMCSGESFSNS*	-2.29	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22544	VWHPPLRAEPFTPSPDNHSA			20	SLAY-screened peptide P894			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTGGCACCCCCCGCTTCGGGCCGAGCCGTTCACTCCGTCTCCTGATAACCATTCGGCGTAA	VWHPPLRAEPFTPSPDNHSA*	-2.289	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22545	CPWYIDSSSAGHYFRLTSLM			20	SLAY-screened peptide P895			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCCTGGTACATCGACTCCAGTTCGGCCGGTCACTACTTTAGGCTTACGTCGCTGATGTAA	CPWYIDSSSAGHYFRLTSLM*	-2.289	0.002115	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22546	RSTFHIGDGNNNHRDKSHYL			20	SLAY-screened peptide P896			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTCGACGTTTCACATCGGCGATGGCAATAATAACCACCGTGATAAGTCTCATTATTTGTAA	RSTFHIGDGNNNHRDKSHYL*	-2.287	0.000187	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22547	PLFIVMLWVRRLKNAYAPML			20	SLAY-screened peptide P897			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTCTTTATTGTCATGCTGTGGGTTCGCCGTCTTAAGAATGCCTATGCCCCTATGCTGTAA	PLFIVMLWVRRLKNAYAPML*	-2.287	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22548	LTSAKGTHCEFYPAPYQGIV			20	SLAY-screened peptide P898			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACGAGCGCCAAGGGTACTCATTGTGAGTTTTATCCGGCCCCCTATCAGGGTATTGTCTAA	LTSAKGTHCEFYPAPYQGIV*	-2.287	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22549	NAKVWNRNI			9	SLAY-screened peptide P899			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGCGAAGGTGTGGAATAGGAATATTTAGTAGGACAATTGTCCTAGTAGTAGGGATGACTAA	NAKVWNRNI**DNCPSSRDD*	-2.286	0.001886	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22550	DAPLLSTPFVSNAHSRCTNS			20	SLAY-screened peptide P900			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATGCGCCTCTTCTCTCTACCCCGTTTGTGTCCAACGCTCACAGTCGGTGTACTAACTCTTAA	DAPLLSTPFVSNAHSRCTNS*	-2.286	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22551	TLSRLWRHLRITFTLHIDRL			20	SLAY-screened peptide P901			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTTGTCTAGGCTCTGGCGGCACCTTCGCATCACGTTTACGCTCCATATCGACAGGCTGTAA	TLSRLWRHLRITFTLHIDRL*	-2.286	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22552	HTSAAAPSHTSHTIANADES			20	SLAY-screened peptide P902			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACTAGTGCTGCTGCGCCGAGCCACACGAGCCATACTATCGCTAACGCTGACGAGAGCTAA	HTSAAAPSHTSHTIANADES*	-2.284	0.001094	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22553	IRHGLSSATNLRATPSVPISN			21	SLAY-screened peptide P903			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCGCCATGGTTTGTCGTCTGCCACCAATCTGAGGGCCACTCCTTCAGTTCCGATTAGTAAC	IRHGLSSATNLRATPSVPISN	-2.281	0.000358	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22554	PLLRPWDIQNHILGEYCNPC			20	SLAY-screened peptide P904			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTGCTGCGGCCTTGGGACATCCAGAATCACATCCTGGGTGAGTATTGCAATCCTTGTTAA	PLLRPWDIQNHILGEYCNPC*	-2.281	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22555	IYSKFTYSVNNIPGRSAFAA			20	SLAY-screened peptide P905			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTACAGCAAGTTTACCTACAGTGTTAACAACATCCCCGGCAGGAGTGCCTTTGCCGCTTAA	IYSKFTYSVNNIPGRSAFAA*	-2.28	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22556	STQNNWSLCLSSHTYNSFFT			20	SLAY-screened peptide P906			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTACTCAGAATAATTGGTCGCTCTGTTTGTCCTCCCATACTTATAACAGTTTTTTTACTTAA	STQNNWSLCLSSHTYNSFFT*	-2.28	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22557	CTSYPFLMPLQFLPLGQAWV			20	SLAY-screened peptide P907			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTACGAGCTATCCTTTTTTGATGCCTTTGCAGTTTCTTCCCCTCGGCCAGGCTTGGGTTTAA	CTSYPFLMPLQFLPLGQAWV*	-2.278	0.001043	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22558	TYRNLDTDE			9	SLAY-screened peptide P908			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTACCGTAACCTCGACACGGACGAGTAGCAGGGTAACGCGCTTCCTATTTAGCATGCGTAA	TYRNLDTDE*QGNALPI*HA*	-2.278	0.000172	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22559	RARSTPHNPRNIFRRKFDCL			20	SLAY-screened peptide P909			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGCGCGGTCTACCCCTCATAACCCTAGGAACATCTTTAGGCGGAAGTTTGACTGTCTGTAA	RARSTPHNPRNIFRRKFDCL*	-2.278	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22560	PDSKVYHDCSALYNNTYIML			20	SLAY-screened peptide P910			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGACTCCAAGGTTTACCACGATTGTTCCGCGCTGTATAATAATACGTATATCATGTTGTAA	PDSKVYHDCSALYNNTYIML*	-2.278	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22561	YTDVIILVARPRRSIRSTPRN			21	SLAY-screened peptide P911			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACGGACGTCATAATTCTGGTGGCCCGACCACGCAGAAGCATACGGAGCACTCCGCGTAAC	YTDVIILVARPRRSIRSTPRN	-2.278	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22562	VHLDSLYPVYEYHKYINLNF			20	SLAY-screened peptide P912			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCACCTTGACTCTCTCTATCCCGTTTACGAGTACCACAAGTACATTAACTTGAATTTTTAA	VHLDSLYPVYEYHKYINLNF*	-2.277	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22563	STSGMYNSLYSLTCRTYTYN			20	SLAY-screened peptide P913			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTACTAGTGGCATGTACAATAGTCTTTATAGCCTCACCTGCCGTACCTATACTTATAATTAA	STSGMYNSLYSLTCRTYTYN*	-2.275	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22564	RPDCNKYTKHLYSFQTKAIG			20	SLAY-screened peptide P914			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCTGATTGCAATAAGTACACTAAGCATCTCTACAGCTTCCAGACGAAGGCTATCGGCTAA	RPDCNKYTKHLYSFQTKAIG*	-2.274	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22565	KNLSFTCNPSQYRIVMLKHLT			21	SLAY-screened peptide P915			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGAATCTCTCTTTCACTTGTAACCCTAGCCAGTACCGCATCGTTATGTTGAAACATTTAACT	KNLSFTCNPSQYRIVMLKHLT	-2.273	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22566	SRNSFDTTSHPIPS			14	SLAY-screened peptide P916			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCGCAACTCGTTCGATACTACTTCTCATCCTATTCCGAGTTAGCGCCTGGGTATCTACTAA	SRNSFDTTSHPIPS*RLGIY*	-2.272	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22567	HSHPLWDNF			9	SLAY-screened peptide P917			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTCTCATCCTTTGTGGGATAATTTCTAGCCGCCTAACAACGTTTATGCGCTGCTTCACTAA	HSHPLWDNF*PPNNVYALLH*	-2.271	0.000317	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22568	DIKPRCYLGHLYGYLNHDFG			20	SLAY-screened peptide P918			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACATTAAGCCGCGCTGCTATCTTGGCCATCTGTACGGTTATCTTAACCATGACTTCGGGTAA	DIKPRCYLGHLYGYLNHDFG*	-2.27	0.000829	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22569	VS			2	SLAY-screened peptide P919			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTCTTAGGGCGTTCGCAACCTTGTTGAGAATTAGGTGTATCATGTCATGCATACCTAACTG	VS*GVRNLVEN*VYHVMHT*L	-2.269	0.000136	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22570	AGSSYLPHTCCRCCPHVEYV			20	SLAY-screened peptide P920			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGGCAGCTCGTACTTGCCTCACACCTGCTGCCGTTGCTGCCCCCACGTCGAGTACGTTTAA	AGSSYLPHTCCRCCPHVEYV*	-2.269	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22571	TFSRAPHPYSTSAAVLSLLS			20	SLAY-screened peptide P921			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTTCTCTCGTGCTCCGCACCCCTACAGCACTTCCGCTGCGGTCCTTTCCCTGCTCTCCTAA	TFSRAPHPYSTSAAVLSLLS*	-2.268	0.000114	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22572	LSNSTLASTLPRYTASRDKI			20	SLAY-screened peptide P922			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCTAACAGTACCCTCGCGTCGACGCTCCCGCGTTACACTGCTTCGCGCGACAAGATCTAA	LSNSTLASTLPRYTASRDKI*	-2.268	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22573	YLLSLDNDTHHIVYHSGEAR			20	SLAY-screened peptide P923			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCTTCTGAGCCTTGACAATGATACTCATCATATCGTTTATCATAGTGGCGAGGCTCGGTAA	YLLSLDNDTHHIVYHSGEAR*	-2.268	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22574	PCPTDSWRWPLILTNVQSVV			20	SLAY-screened peptide P924			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGCCCCACCGACAGCTGGCGGTGGCCTCTCATCTTGACCAACGTTCAGTCCGTCGTGTAA	PCPTDSWRWPLILTNVQSVV*	-2.266	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22575	YNRDDYFRNYHYRCLYPTLS			20	SLAY-screened peptide P925			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACAACCGTGACGATTATTTTAGGAATTACCATTACCGCTGCCTCTATCCCACGCTTAGCTAA	YNRDDYFRNYHYRCLYPTLS*	-2.265	0.001245	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22576	PDIIRAFNTIPDRIFGYASQ			20	SLAY-screened peptide P926			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGACATTATCCGCGCTTTCAACACTATTCCTGACCGTATTTTTGGCTATGCTTCCCAGTAA	PDIIRAFNTIPDRIFGYASQ*	-2.262	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22577	SCSHIPSRDFWTHSHTIQRA			20	SLAY-screened peptide P927			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTGCTCCCACATCCCCTCGCGCGACTTCTGGACCCATTCGCACACTATCCAGCGTGCCTAA	SCSHIPSRDFWTHSHTIQRA*	-2.262	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22578	NRFGYLDPVSSEFDIFGRRP			20	SLAY-screened peptide P928			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCGTTTTGGTTATCTTGATCCTGTCAGCAGTGAGTTTGACATCTTTGGCCGCCGTCCGTAA	NRFGYLDPVSSEFDIFGRRP*	-2.262	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22579	PLLQVCVPYIPWKGLYFYRC			20	SLAY-screened peptide P929			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTGCTCCAGGTTTGTGTTCCGTATATTCCTTGGAAGGGCCTGTATTTCTACCGTTGTTAA	PLLQVCVPYIPWKGLYFYRC*	-2.262	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22580	LLISFLPAAMTYPRSPRSAS			20	SLAY-screened peptide P930			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTTATTTCCTTCCTTCCCGCGGCGATGACCTACCCTCGCAGCCCTCGTAGTGCTTCCTAA	LLISFLPAAMTYPRSPRSAS*	-2.26	4.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22581	FHHLPVALWFPKPVLSLGTC			20	SLAY-screened peptide P931			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCACCATCTTCCCGTGGCCTTGTGGTTCCCTAAGCCGGTCTTGTCTCTGGGTACTTGTTAA	FHHLPVALWFPKPVLSLGTC*	-2.259	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22582	APNLKLAFPRHLMFTINNTC			20	SLAY-screened peptide P932			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCCAACCTTAAGTTGGCGTTTCCCCGTCATCTTATGTTCACTATTAATAACACCTGTTAA	APNLKLAFPRHLMFTINNTC*	-2.259	0.000342	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22583	PNKTIWITTTASRVRLGSLTN			21	SLAY-screened peptide P933			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAACAAGACTATCTGGATCACAACTACAGCCTCCCGTGTCAGATTAGGATCCCTAACTAAC	PNKTIWITTTASRVRLGSLTN	-2.258	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22584	PMDTDSPLTSSNRAYHLVQ			19	SLAY-screened peptide P934			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATGGATACTGATAGTCCTCTGACTTCCTCTAACCGTGCGTACCACCTTGTTCAGTAACTG	PMDTDSPLTSSNRAYHLVQ*L	-2.258	0.000156	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22585	EDDSKLILSVPWFHLYRRRRN			21	SLAY-screened peptide P935			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGACGACTCCAAGCTGATCCTCAGTGTCCCGTGGTTCCATCTCTACCGGCGTAGGCGTAAC	EDDSKLILSVPWFHLYRRRRN	-2.258	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22586	LQALVSSSLPRTRYLHAYCV			20	SLAY-screened peptide P936			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCAGGCCCTTGTTAGTAGTTCTCTTCCGCGTACTCGTTACTTGCACGCTTATTGCGTCTAA	LQALVSSSLPRTRYLHAYCV*	-2.258	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22587	LSHSFTDNLFN			11	SLAY-screened peptide P937			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTCTCACAGTTTTACTGATAATTTGTTCAATTAGTTTGTTCCGACCTGTACCGGCCAGTAA	LSHSFTDNLFN*FVPTCTGQ*	-2.258	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22588	NPGQRSNSDQSY			12	SLAY-screened peptide P938			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCCCGGGCAGAGGTCGAACTCGGATCAGAGTTACTAGTTTTCCGATAAGGAGCACTGCTAA	NPGQRSNSDQSY*FSDKEHC*	-2.257	0.000438	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22589	FPNTANFVLCYKTLCLKYSD			20	SLAY-screened peptide P939			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCCTAACACTGCTAACTTTGTTTTGTGCTATAAGACGCTCTGCCTCAAGTATTCTGACTAA	FPNTANFVLCYKTLCLKYSD*	-2.256	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22590	TIVSVP			6	SLAY-screened peptide P940			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTATCGTTTCTGTTCCTTAGCAGGCTGTCAGTTACATTTTCGGCTATAATTAGCTTGAGTAA	TIVSVP*QAVSYIFGYN*LE*	-2.256	0.000182	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22591	WPLNMHHPNLIISYTSSYRL			20	SLAY-screened peptide P941			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCCTCTCAACATGCATCATCCTAATTTGATTATTAGCTACACGTCTTCGTACCGCCTCTAA	WPLNMHHPNLIISYTSSYRL*	-2.256	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22592	SLCHFLTNPPPESSRYT			17	SLAY-screened peptide P942			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCTTTGCCATTTTCTTACTAACCCGCCTCCTGAGTCTTCGCGTTATACGTAGCTTATGTAA	SLCHFLTNPPPESSRYT*LM*	-2.255	1.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22593	PSDFAATCWRYIKKARHVFL			20	SLAY-screened peptide P943			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCTGATTTTGCTGCCACTTGCTGGCGCTACATCAAGAAGGCTCGTCATGTTTTTCTTTAA	PSDFAATCWRYIKKARHVFL*	-2.255	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22594	LHYLDGFYNDNTTYLMLQAP			20	SLAY-screened peptide P944			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCACTATCTGGACGGTTTCTATAATGATAATACCACTTACTTGATGCTCCAGGCTCCCTAA	LHYLDGFYNDNTTYLMLQAP*	-2.254	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22595	TLGRLQLPVPFIGDLAHNNM			20	SLAY-screened peptide P945			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTGGGCCGCCTGCAGCTTCCCGTCCCCTTCATTGGGGATCTTGCCCACAATAATATGTAA	TLGRLQLPVPFIGDLAHNNM*	-2.254	0.001787	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22596	PCPFLPPIHSCSKGLQRYNH			20	SLAY-screened peptide P946			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGCCCCTTTCTGCCGCCTATCCATTCCTGCTCTAAGGGGCTTCAGAGGTATAATCATTAA	PCPFLPPIHSCSKGLQRYNH*	-2.254	7.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22597	PLRDHWSNLHHMSVPNWGLC			20	SLAY-screened peptide P947			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCCGGGATCACTGGTCTAACCTTCACCACATGTCTGTTCCTAATTGGGGGCTGTGTTAA	PLRDHWSNLHHMSVPNWGLC*	-2.251	3.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22598	AMHVLLHRASFYTSAFWHA			19	SLAY-screened peptide P948			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGATGCATGTTCTTCTCCATCGTGCTTCTTTTTATACCTCGGCCTTTTGGCACGCTTAGTAA	AMHVLLHRASFYTSAFWHA**	-2.251	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22599	PICHYPYPALQPLHRPYRAAL			21	SLAY-screened peptide P949			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATCTGCCATTACCCTTACCCTGCGCTTCAGCCTCTGCATCGGCCATATCGTGCCGCCCTA	PICHYPYPALQPLHRPYRAAL	-2.251	2.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22600	LQTWLFKAESCIIHLSHCET			20	SLAY-screened peptide P950			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCAGACGTGGCTTTTTAAGGCTGAGAGCTGTATTATTCACTTGTCCCACTGTGAGACCTAA	LQTWLFKAESCIIHLSHCET*	-2.25	9.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22601	SLPYLHLSLNLNSGYTGNAP			20	SLAY-screened peptide P951			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCTGCCGTACCTTCACCTGTCGCTGAACCTCAATTCCGGTTACACTGGCAACGCCCCGTAA	SLPYLHLSLNLNSGYTGNAP*	-2.249	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22602	LLYFLLLYNNRTPECRRGPSN			21	SLAY-screened peptide P952			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCTCTACTTCCTCCTTTTGTATAATAATAGGACTCCCGAATGTCGAAGAGGCCCCTCTAAC	LLYFLLLYNNRTPECRRGPSN	-2.249	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22603	GNPAYSSIHTVGLRKHKNTD			20	SLAY-screened peptide P953			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTAATCCTGCCTATAGCTCCATCCACACCGTTGGTCTGCGTAAGCATAAGAATACTGATTAA	GNPAYSSIHTVGLRKHKNTD*	-2.248	0.00251	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22604	TCRPFSAAYLFDDRHNIRPT			20	SLAY-screened peptide P954			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTGTCGCCCTTTCAGTGCCGCTTATCTTTTTGATGATCGGCACAATATCCGCCCTACTTAA	TCRPFSAAYLFDDRHNIRPT*	-2.247	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22605	PPHCRSRSGYCFRIRAY			17	SLAY-screened peptide P955			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCCCATTGTCGTAGCCGCTCGGGGTATTGCTTCCGTATTCGCGCGTACTAGATTACGTAA	PPHCRSRSGYCFRIRAY*IT*	-2.247	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22606	HYCAVTTPHVRCLATT			16	SLAY-screened peptide P956			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTATTGTGCTGTTACTACTCCTCATGTGCGGTGCCTGGCTACCACCTAGCCCGGCTATTAA	HYCAVTTPHVRCLATT*PGY*	-2.246	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22607	PDDI			4	SLAY-screened peptide P957			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGATGATATTTAGCCTCTTATGCACAATTACATTACCATTCCCTTGCCGCATCTCCATTAA	PDDI*PLMHNYITIPLPHLH*	-2.245	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22608	PSHDQRTILMLNLMYALQCV			20	SLAY-screened peptide P958			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCCCATGACCAGAGGACGATCCTTATGTTGAATCTTATGTATGCTCTCCAGTGTGTGTAA	PSHDQRTILMLNLMYALQCV*	-2.245	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22609	SRPIYTWIYSPIPLYNAFKT			20	SLAY-screened peptide P959			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCGTCCCATCTATACTTGGATTTATAGCCCCATCCCCTTGTACAACGCGTTTAAGACCTAA	SRPIYTWIYSPIPLYNAFKT*	-2.244	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22610	ILYRKNFGCVAHIVGVVRAV			20	SLAY-screened peptide P960			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCTTTACCGCAAGAATTTTGGTTGTGTCGCTCACATTGTGGGTGTTGTGCGGGCTGTCTAA	ILYRKNFGCVAHIVGVVRAV*	-2.243	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22611	RTC			3	SLAY-screened peptide P961			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACTTGTTAGAACTCCTATTGCCGCCTTAGCCACCAGGGCCTTAATAATCTTCGTAACTAA	RTC*NSYCRLSHQGLNNLRN*	-2.243	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22612	NCNLESCSYDRTGLDGPD			18	SLAY-screened peptide P962			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTGTAACCTTGAGAGTTGCTCTTATGATAGAACAGGTCTAGATGGGCCTGACTGATAACTG	NCNLESCSYDRTGLDGPD**L	-2.242	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22613	CHTSNTSPGVKLASNPPNTD			20	SLAY-screened peptide P963			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCACACCTCTAACACTAGCCCGGGCGTTAAGTTGGCTTCTAATCCGCCGAATACCGACTAA	CHTSNTSPGVKLASNPPNTD*	-2.242	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22614	PDFSKTTLCGPTASETADPL			20	SLAY-screened peptide P964			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGATTTTAGTAAGACGACGCTTTGCGGTCCGACCGCTTCTGAGACTGCGGATCCCCTTTAA	PDFSKTTLCGPTASETADPL*	-2.242	0.000132	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22615	ACYAHTNGIHPEHNATHMCD			20	SLAY-screened peptide P965			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTGCTACGCCCACACTAACGGTATTCATCCTGAGCATAACGCTACCCACATGTGCGATTAA	ACYAHTNGIHPEHNATHMCD*	-2.241	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22616	RRTNSRAFTTLLRRVALKTM			20	SLAY-screened peptide P966			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGTACCAACTCCAGGGCTTTCACCACGCTGCTGAGGCGTGTCGCCCTTAAGACCATGTAA	RRTNSRAFTTLLRRVALKTM*	-2.24	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22617	SRPRLATSCSHLLTSALPSTN			21	SLAY-screened peptide P967			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCGTCCTCGGCTTGCTACCTCTTGCTCCCATCTACTTACAAGCGCACTACCTTCAACTAAC	SRPRLATSCSHLLTSALPSTN	-2.238	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22618	IILIFLRNRPRGC			13	SLAY-screened peptide P968			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTATTCTCATCTTCCTTCGTAACCGTCCGAGGGGGTGTTAGACGCACAGCGACTCCTGCTAA	IILIFLRNRPRGC*THSDSC*	-2.238	0.002889	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22619	SNRFYTMPHHTPTELEPRSP			20	SLAY-screened peptide P969			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAACCGGTTCTACACCATGCCGCACCACACTCCTACTGAGCTTGAGCCTCGTAGCCCGTAA	SNRFYTMPHHTPTELEPRSP*	-2.237	0.000227	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22620	RPIFLSTAYRTDYYPITYSV			20	SLAY-screened peptide P970			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCCATCTTCTTGTCCACGGCGTACCGTACTGATTATTACCCGATCACGTACTCCGTTTAA	RPIFLSTAYRTDYYPITYSV*	-2.236	0.002588	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22621	HFYTDIYKALRIGHNATKLH			20	SLAY-screened peptide P971			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTTTTACACTGATATTTACAAGGCTCTCCGTATCGGTCACAACGCCACTAAGCTGCACTAA	HFYTDIYKALRIGHNATKLH*	-2.236	4.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22622	PTVGVWSLLPYRCTNIYTRA			20	SLAY-screened peptide P972			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACCGTCGGGGTCTGGTCCCTCCTGCCGTATCGGTGCACCAATATCTACACGCGTGCTTAA	PTVGVWSLLPYRCTNIYTRA*	-2.235	0.003337	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22623	GHQLSNPPFSPNLRTHTYGL			20	SLAY-screened peptide P973			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCATCAGCTGTCCAATCCGCCGTTCAGCCCGAACCTTCGTACGCACACGTATGGTCTGTAA	GHQLSNPPFSPNLRTHTYGL*	-2.235	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22624	RLSCDYRRAACLEHNFSLFQY			21	SLAY-screened peptide P974			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTTTCGTGCGACTATCGGCGTGCCGCGTGTCTTGAGCATAACTTTTCGCTGTTCCAGTAC	RLSCDYRRAACLEHNFSLFQY	-2.234	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22625	RDYKLTFTYFSSHGHSR			17	SLAY-screened peptide P975			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGACTATAAGCTCACTTTTACTTACTTTTCGAGTCACGGGCATTCTCGGTAGCGTAGTTAA	RDYKLTFTYFSSHGHSR*RS*	-2.234	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22626	SSYLITDSDIDSVPDSFIST			20	SLAY-screened peptide P976			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCAGTTATCTTATCACTGACAGCGATATTGACTCTGTTCCTGATTCTTTCATTTCCACCTAA	SSYLITDSDIDSVPDSFIST*	-2.233	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22627	TQHSLHEPGTDLASSTTFL			19	SLAY-screened peptide P977			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCAGCACTCCCTCCATGAGCCCGGTACGGACCTGGCCAGTTCTACTACTTTTCTCTAGCTC	TQHSLHEPGTDLASSTTFL*L	-2.231	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22628	LRHPRDLVGSDTVTLHHFIS			20	SLAY-screened peptide P978			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAGGCATCCCCGTGATCTCGTTGGTTCTGATACTGTCACTCTGCATCATTTTATTTCCTAA	LRHPRDLVGSDTVTLHHFIS*	-2.23	0.000243	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22629	TARI			4	SLAY-screened peptide P979			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGCCCGTATTTAGATTAACCCTGACAGCAATCTTATTATCAACAGCCCCTTTTAGTACTAA	TARI*INPDSNLIINSPF*Y*	-2.23	0.000262	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22630	YAYSPHVYAHTFQRDYYRNL			20	SLAY-screened peptide P980			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGCCTATTCCCCTCATGTGTATGCTCATACGTTTCAGAGGGACTACTATAGGAATCTGTAA	YAYSPHVYAHTFQRDYYRNL*	-2.229	0.000633	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22631	ATKPLSNTMHTN			12	SLAY-screened peptide P981			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTACCAAGCCCCTTTCTAACACGATGCATACTAATTAGTTTAACAGCGTTACGGTCCAGTAA	ATKPLSNTMHTN*FNSVTVQ*	-2.229	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22632	LHNSTIGENFR			11	SLAY-screened peptide P982			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCATAATAGCACCATCGGCGAGAATTTTCGTTAGTTTTCCCATTATACGATGACTATGTAA	LHNSTIGENFR*FSHYTMTM*	-2.229	6.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22633	IARALRSYVGLFAVAQ			16	SLAY-screened peptide P983			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGCGCGGGCACTGCGCTCTTATGTGGGCCTTTTTGCAGTAGCTCAGTAGTAAGGTCTAACT	IARALRSYVGLFAVAQ**GLT	-2.229	0.00089	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22634	PPCAFIPMLARHQQSTRTLE			20	SLAY-screened peptide P984			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCCTGTGCGTTTATTCCGATGTTGGCTCGTCACCAGCAGTCCACTAGGACTCTGGAGTAA	PPCAFIPMLARHQQSTRTLE*	-2.229	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22635	PYNCKNTWSLRSHIRQALHN			20	SLAY-screened peptide P985			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTACAATTGTAAGAATACGTGGTCCTTGCGGTCGCATATCAGGCAGGCGCTCCATAACTAA	PYNCKNTWSLRSHIRQALHN*	-2.228	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22636	RITRRSRGYLPDRDGTVSNR			20	SLAY-screened peptide P986			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTATTACCAGGCGTTCTAGGGGTTATTTGCCTGATAGGGACGGCACGGTCTCGAACCGTTAA	RITRRSRGYLPDRDGTVSNR*	-2.226	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22637	YSPSDPPHYSVLPFYEIAAA			20	SLAY-screened peptide P987			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTCCCCGAGTGATCCTCCGCATTACTCGGTGCTGCCCTTTTATGAGATTGCTGCTGCTTAA	YSPSDPPHYSVLPFYEIAAA*	-2.225	0.000232	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22638	LPTACDYVGHHNDKTMTRLY			20	SLAY-screened peptide P988			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCCACGGCGTGCGACTATGTCGGTCACCATAACGACAAGACCATGACCCGTCTTTACTAA	LPTACDYVGHHNDKTMTRLY*	-2.225	0.000454	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22639	LVTW			4	SLAY-screened peptide P989			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGTTACTTGGTAGCACCACTTTTGCCACCAGTTGTCTCACCAGATTAATTATTTTTGTTAA	LVTW*HHFCHQLSHQINYFC*	-2.225	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22640	GASHFFPLSFDINVYLC			17	SLAY-screened peptide P990			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGGCTAGTCATTTTTTCCCTTTGTCTTTCGATATTAATGTTTATCTGTGCTAGTCGACCTAA	GASHFFPLSFDINVYLC*ST*	-2.224	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22641	PHRPFWFSLGHSVLSQALAF			20	SLAY-screened peptide P991			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCACCGTCCTTTTTGGTTTAGCCTGGGCCATTCTGTGCTGTCTCAGGCGCTTGCCTTTTAA	PHRPFWFSLGHSVLSQALAF*	-2.224	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22642	LGPSLRCMPLWQYYSI			16	SLAY-screened peptide P992			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGGTCCGTCCCTTCGCTGCATGCCCCTCTGGCAGTACTACTCTATCTAGCGGAGTTACTAA	LGPSLRCMPLWQYYSI*RSY*	-2.223	0.001571	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22643	RHVVLMLHTSDAYTSAPHFS			20	SLAY-screened peptide P993			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCACGTCGTGCTGATGCTGCACACCTCTGACGCGTACACTTCCGCCCCCCATTTCTCCTAA	RHVVLMLHTSDAYTSAPHFS*	-2.222	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22644	DPIYISS			7	SLAY-screened peptide P994			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCCGATCTATATTAGCAGTTAGACTAAGCATAGTACTATCTCCAATTACTCTAACTCTTAA	DPIYISS*TKHSTISNYSNS*	-2.221	0.000193	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22645	LSGIYMWCFVCINSSASPAP			20	SLAY-screened peptide P995			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCTGGTATCTACATGTGGTGTTTCGTTTGTATCAACAGCTCTGCGAGTCCGGCCCCGTAA	LSGIYMWCFVCINSSASPAP*	-2.22	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22646	VHLVNYGLVSRWPPDPTGRP			20	SLAY-screened peptide P996			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCACCTTGTTAATTATGGCTTGGTTTCGCGTTGGCCCCCTGATCCCACCGGTAGGCCCTAA	VHLVNYGLVSRWPPDPTGRP*	-2.22	0.000452	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22647	RRYLRFSGYYLHSTHNKCHY			20	SLAY-screened peptide P997			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGCTATCTTCGCTTCTCCGGGTACTATCTGCATAGTACTCATAACAAGTGTCACTATTAA	RRYLRFSGYYLHSTHNKCHY*	-2.218	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22648	RPPVNSHTSST			11	SLAY-screened peptide P998			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCTCCGGTTAATTCGCACACCAGTAGCACGTAGGCTGCGCCTACCGCTTTGAACGTTTAA	RPPVNSHTSST*AAPTALNV*	-2.218	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22649	YTMNIDSRVENLPIP			15	SLAY-screened peptide P999			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATACCATGAATATCGACAGTAGGGTGGAGAATCTTCCGATCCCTTAGCATTCTATTACTAAC	YTMNIDSRVENLPIP*HSITN	-2.218	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22650	APTNYPVVYYNSTTIEKVSG			20	SLAY-screened peptide P1000			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCCGACGAACTATCCTGTTGTCTACTACAATTCGACGACCATTGAGAAGGTTTCGGGTTAA	APTNYPVVYYNSTTIEKVSG*	-2.217	0.003046	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22651	PTTLRIRVSPFLARCRRARRN			21	SLAY-screened peptide P1001			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACGACGTTGCGGATCAGAGTTAGTCCATTCTTGGCCCGCTGCAGACGCGCGAGACGTAAC	PTTLRIRVSPFLARCRRARRN	-2.216	9.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22652	RVTIYPGAATPVDKRHLTPA			20	SLAY-screened peptide P1002			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGTCACGATTTATCCTGGCGCGGCTACTCCTGTCGACAAGAGGCACCTTACCCCGGCGTAA	RVTIYPGAATPVDKRHLTPA*	-2.215	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22653	RRTSGLAVCNQLHFAICIYN			20	SLAY-screened peptide P1003			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCGTACCTCTGGTCTCGCGGTTTGCAACCAGCTGCACTTTGCTATTTGTATTTATAATTAA	RRTSGLAVCNQLHFAICIYN*	-2.215	0.000199	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22654	PLYGNTVSYPVASLQRYANL			20	SLAY-screened peptide P1004			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTTTATGGCAATACCGTTAGCTATCCCGTGGCGAGTCTTCAGCGCTATGCTAATCTCTAA	PLYGNTVSYPVASLQRYANL*	-2.215	0.000123	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22655	FLDPEPKCIHNRNHNPTPSD			20	SLAY-screened peptide P1005			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTTGGACCCTGAGCCCAAGTGTATCCATAACCGGAATCATAACCCTACCCCCAGTGATTAA	FLDPEPKCIHNRNHNPTPSD*	-2.215	0.000449	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22656	LLIHLCRCPNLCSLVSGNNT			20	SLAY-screened peptide P1006			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCTCATTCACCTCTGCAGGTGCCCCAATCTGTGTAGCCTTGTGAGCGGTAACAATACTTAA	LLIHLCRCPNLCSLVSGNNT*	-2.214	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22657	DALAHAHYSPLNNSHCCYCY			20	SLAY-screened peptide P1007			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATGCCCTCGCTCACGCTCATTATTCTCCTCTGAACAACAGTCATTGTTGTTACTGCTACTAA	DALAHAHYSPLNNSHCCYCY*	-2.214	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22658	PLSSKRNNTHYSHTRAYKIR			20	SLAY-screened peptide P1008			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTTGTCGTCTAAGAGGAATAACACTCATTACTCTCACACGCGCGCTTATAAGATCCGTTAA	PLSSKRNNTHYSHTRAYKIR*	-2.213	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22659	TYIAHCSVGRLYMYYSINRS			20	SLAY-screened peptide P1009			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTACATTGCTCATTGTAGCGTTGGCCGCCTGTATATGTACTACAGCATTAATCGTTCCTAA	TYIAHCSVGRLYMYYSINRS*	-2.21	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22660	YFLSCPLMQANPGTVAT			17	SLAY-screened peptide P1010			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTTTCTTTCCTGTCCGCTCATGCAGGCTAATCCGGGTACCGTCGCGACCTAGGGGCACTAA	YFLSCPLMQANPGTVAT*GH*	-2.208	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22661	PHCDSTIYIRHYPRDNMALT			20	SLAY-screened peptide P1011			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCATTGTGACTCGACCATTTACATCAGGCATTATCCTCGCGATAATATGGCTCTGACCTAA	PHCDSTIYIRHYPRDNMALT*	-2.208	1.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22662	QHTHSRKADTCITPMPCSAD			20	SLAY-screened peptide P1012			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCATACTCACTCTCGTAAGGCCGATACTTGCATTACGCCCATGCCGTGTAGTGCCGATTAA	QHTHSRKADTCITPMPCSAD*	-2.208	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22663	TTRHSHVKTCFYTGVPLHPL			20	SLAY-screened peptide P1013			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACTCGCCATTCGCACGTTAAGACTTGCTTCTATACTGGCGTCCCTCTTCACCCCCTCTAA	TTRHSHVKTCFYTGVPLHPL*	-2.208	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22664	TVLLPCGPFHVLY			13	SLAY-screened peptide P1014			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTTCTCCTTCCGTGCGGCCCTTTTCACGTTTTGTATTAGCATCCGGATGAGAATCTCTAA	TVLLPCGPFHVLY*HPDENL*	-2.207	5.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22665	CYSSLLLRFWLFVKALSAQF			20	SLAY-screened peptide P1015			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTATTCGTCTCTGCTTCTCCGCTTTTGGCTTTTCGTTAAGGCGCTGAGTGCCCAGTTTTAA	CYSSLLLRFWLFVKALSAQF*	-2.206	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22666	RPSFPGLHLPVVVSRLSCSIN			21	SLAY-screened peptide P1016			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCGTCTTTCCCTGGGTTGCATCTTCCTGTTGTCGTAAGCCGCCTGTCCTGCAGTATTAAC	RPSFPGLHLPVVVSRLSCSIN	-2.206	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22667	PPCLSYSATRRRYCCLPQIT			20	SLAY-screened peptide P1017			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCGTGTCTCAGTTATTCTGCTACCCGTCGGCGGTATTGCTGTTTGCCTCAGATCACTTAA	PPCLSYSATRRRYCCLPQIT*	-2.205	0.001147	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22668	NDFDATHLRSDRKNITYWLL			20	SLAY-screened peptide P1018			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGACTTTGATGCTACCCACCTCAGGAGTGATAGGAAGAATATTACCTACTGGCTTCTGTAA	NDFDATHLRSDRKNITYWLL*	-2.2	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22669	IGPLSCTPLLFIAWNSESVS			20	SLAY-screened peptide P1019			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGGCCCGCTTAGTTGTACTCCCCTCCTTTTTATCGCTTGGAACAGTGAGTCCGTTTCGTAA	IGPLSCTPLLFIAWNSESVS*	-2.2	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22670	NCRSVLVYYGTPGTRSYHCY			20	SLAY-screened peptide P1020			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTGCCGCAGTGTCCTTGTGTATTACGGCACTCCTGGTACTCGTAGTTATCATTGCTATTAA	NCRSVLVYYGTPGTRSYHCY*	-2.197	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22671	AYCVASAALAHLYVWTMTIP			20	SLAY-screened peptide P1021			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTACTGCGTGGCGTCCGCGGCCCTCGCCCACCTTTATGTCTGGACTATGACTATCCCGTAA	AYCVASAALAHLYVWTMTIP*	-2.197	0.003325	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22672	IDSPAYVTLCVPRRRKQAYN			20	SLAY-screened peptide P1022			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGACAGTCCCGCCTACGTCACCCTTTGCGTTCCGCGGAGGAGGAAGCAGGCTTACAACTAA	IDSPAYVTLCVPRRRKQAYN*	-2.196	0.000504	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22673	TVQINSADPYCI			12	SLAY-screened peptide P1023			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGTTCAGATCAATAGTGCCGACCCCTATTGCATCTAGGATGATCTGCATCATTCTGACTAA	TVQINSADPYCI*DDLHHSD*	-2.196	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22674	LLS			3	SLAY-screened peptide P1024			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTCTCGTAGCTGATCTTTGGTGTGAGTGGTCACGCCGCGGCTAACGGCTTCGATTCCTAA	LLS*LIFGVSGHAAANGFDS*	-2.196	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22675	TSATTCVYYRPRRHYCSMAS			20	SLAY-screened peptide P1025			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAGCGCCACGACTTGCGTTTATTATCGGCCGCGCAGGCACTATTGTTCGATGGCGTCCTAA	TSATTCVYYRPRRHYCSMAS*	-2.196	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22676	HNFGQDLAYFHQAYFNGGGG			20	SLAY-screened peptide P1026			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAACTTTGGGCAGGACTTGGCCTATTTTCATCAGGCTTATTTCAATGGCGGTGGTGGCTAA	HNFGQDLAYFHQAYFNGGGG*	-2.195	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22677	HGLRLPAGGTDVCDRHAPRT			20	SLAY-screened peptide P1027			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGGCCTGCGCCTGCCGGCGGGTGGGACCGATGTCTGTGACCGTCATGCTCCTCGTACGTAA	HGLRLPAGGTDVCDRHAPRT*	-2.195	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22678	HGFSLHFTLLDSSIYVSILN			20	SLAY-screened peptide P1028			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGGGTTCTCGCTCCATTTTACCCTTTTGGATAGCTCTATCTACGTTAGTATCCTTAATTAA	HGFSLHFTLLDSSIYVSILN*	-2.194	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22679	PPFDVKDVPGLLVP			14	SLAY-screened peptide P1029			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCTTTCGACGTTAAGGACGTTCCGGGCCTCTTGGTCCCTTAGCGTTAGTTCTTGCACTAA	PPFDVKDVPGLLVP*R*FLH*	-2.194	0.00015	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22680	SHKTDQSTDDGVRDTRYSNS			20	SLAY-screened peptide P1030			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCACAAGACTGACCAGTCGACGGACGATGGTGTCAGGGATACTCGTTATTCGAATAGTTAA	SHKTDQSTDDGVRDTRYSNS*	-2.193	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22681	PGQLSIYLRMYRIKRTRCRV			20	SLAY-screened peptide P1031			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGGTCAGCTTTCCATCTACCTTCGCATGTATCGCATTAAGCGCACGCGCTGCCGGGTCTAA	PGQLSIYLRMYRIKRTRCRV*	-2.193	0.000593	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22682	PEFSLSNNSPCSTINALERL			20	SLAY-screened peptide P1032			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGAGTTTAGCCTTAGCAATAATAGCCCGTGCAGTACGATTAATGCGCTGGAGAGGTTGTAA	PEFSLSNNSPCSTINALERL*	-2.192	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22683	QFATFARASLIPNLPRVGPL			20	SLAY-screened peptide P1033			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTTCGCCACCTTCGCGCGGGCCTCCCTGATCCCTAATCTTCCTAGGGTCGGTCCCCTTTAA	QFATFARASLIPNLPRVGPL*	-2.192	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22684	HRTNYMSKYHD			11	SLAY-screened peptide P1034			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCGTACTAATTATATGTCTAAGTATCATGATTAGATGGCCACGTAGCTTTTCATCTTGTGA	HRTNYMSKYHD*MAT*LFIL*	-2.192	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22685	HVHQ			4	SLAY-screened peptide P1035			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGTGCACCAGTAGGTCTCCACCTATACTAATATTACGAATTAGACCGGCTGTATGCAGTAA	HVHQ*VSTYTNITN*TGCMQ*	-2.191	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22686	NCTRRVNATITLAGLRMLIR			20	SLAY-screened peptide P1036			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTGTACGCGGCGCGTGAATGCTACTATCACCCTGGCGGGCCTCCGGATGCTGATTCGTTAA	NCTRRVNATITLAGLRMLIR*	-2.189	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22687	LNNI			4	SLAY-screened peptide P1037			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAACAATATCTAGTACCCTGAGTGGCCCTGTAATATTCCCGTTCCGCGGCCGTCTAATTAA	LNNI*YPEWPCNIPVPRPSN*	-2.189	4.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22688	DPCQGVAVSPPFPRIAIGRAN			21	SLAY-screened peptide P1038			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCCGTGTCAGGGGGTTGCGGTGTCACCACCATTTCCGCGAATTGCAATTGGGAGGGCTAAC	DPCQGVAVSPPFPRIAIGRAN	-2.189	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22689	NFIRPADGHPSCVARFHDAI			20	SLAY-screened peptide P1039			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTTCATCCGTCCGGCCGATGGTCACCCGTCCTGTGTTGCCCGGTTTCATGACGCCATCTAA	NFIRPADGHPSCVARFHDAI*	-2.188	0.000323	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22690	LSVAPDRFVIQTLSLHTHQG			20	SLAY-screened peptide P1040			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCGGTTGCCCCCGATCGCTTTGTTATTCAGACGCTTAGCCTCCATACCCATCAGGGTTAA	LSVAPDRFVIQTLSLHTHQG*	-2.187	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22691	FNLILLRRLAAWRRKSYTPH			20	SLAY-screened peptide P1041			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAACTTGATTCTGCTGCGTCGTTTGGCCGCTTGGCGCAGGAAGTCGTATACGCCTCATTAA	FNLILLRRLAAWRRKSYTPH*	-2.187	0.0014	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22692	PDPVNANTNRIMCLVYYNSM			20	SLAY-screened peptide P1042			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGACCCGGTCAACGCCAACACTAATCGTATTATGTGCCTTGTGTACTATAATTCTATGTAA	PDPVNANTNRIMCLVYYNSM*	-2.186	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22693	LAHPPPVPHNRWHSFTTYLL			20	SLAY-screened peptide P1043			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGCCCATCCCCCTCCCGTTCCCCATAACCGGTGGCATTCTTTTACTACTTATCTCCTCTAA	LAHPPPVPHNRWHSFTTYLL*	-2.186	0.001717	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22694	RFLVHAFHPLNPPLTLVHNE			20	SLAY-screened peptide P1044			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTTCCTTGTTCATGCGTTTCATCCGCTTAATCCTCCTTTGACGCTGGTGCACAACGAGTAA	RFLVHAFHPLNPPLTLVHNE*	-2.186	5.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22695	GSTCHSRHDNHILDHSFNSR			20	SLAY-screened peptide P1045			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGTCTACCTGCCACTCTCGCCATGATAATCATATCCTTGACCACAGCTTTAACTCCAGGTAA	GSTCHSRHDNHILDHSFNSR*	-2.186	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22696	CLDYVDTETCSPCFNRTVTSN			21	SLAY-screened peptide P1046			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTTGGATTACGTCGATACTGAGACCTGCAGTCCCTGTTTTAATAGAACAGTTACGAGTAAC	CLDYVDTETCSPCFNRTVTSN	-2.186	0.000408	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22697	HNLTYTFLSSCPWVYSVSYH			20	SLAY-screened peptide P1047			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAACCTTACGTATACCTTTCTCTCTTCGTGTCCTTGGGTATATAGCGTCTCGTATCACTAA	HNLTYTFLSSCPWVYSVSYH*	-2.185	0.002589	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22698	SPYKRLMLILIFIILISVRIN			21	SLAY-screened peptide P1048			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCCCTACAAGAGACTGATGCTTATCCTAATATTTATCATACTAATCAGCGTGCGGATTAAC	SPYKRLMLILIFIILISVRIN	-2.184	3.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22699	YTGRFNGHNCKCGALCLDYI			20	SLAY-screened peptide P1049			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACGGGCCGTTTTAACGGCCACAACTGTAAGTGTGGTGCTCTTTGTCTTGATTACATTTAA	YTGRFNGHNCKCGALCLDYI*	-2.184	0.005556	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22700	AKRGTPLICFGLFTRMDRRL			20	SLAY-screened peptide P1050			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAAGCGCGGGACTCCTCTCATCTGTTTTGGGCTTTTTACTAGGATGGATAGGCGGTTGTAA	AKRGTPLICFGLFTRMDRRL*	-2.183	5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22701	IGDNGYFFPSKHIRSNFPD			19	SLAY-screened peptide P1051			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGGGGACAACGGTTACTTTTTCCCTTCCAAGCATATCCGTAGTAATTTCCCTGACTAGTAA	IGDNGYFFPSKHIRSNFPD**	-2.183	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22702	TGQTHANAHRHNPYFSTLDV			20	SLAY-screened peptide P1052			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGGCCAGACCCACGCTAACGCTCACAGGCACAACCCCTACTTTTCCACCCTCGACGTGTAA	TGQTHANAHRHNPYFSTLDV*	-2.183	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22703	YSHAFMASFGHCAKSYYLVP			20	SLAY-screened peptide P1053			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTCCCATGCCTTCATGGCCTCGTTTGGCCATTGTGCTAAGAGTTACTATCTGGTCCCCTAA	YSHAFMASFGHCAKSYYLVP*	-2.183	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22704	QATSYARTTNFLPILLICGH			20	SLAY-screened peptide P1054			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGCTACCAGCTATGCGCGTACTACCAACTTTCTGCCCATCCTCCTCATTTGCGGCCATTAA	QATSYARTTNFLPILLICGH*	-2.182	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22705	PLTISSNYRNVVGESGSTSI			20	SLAY-screened peptide P1055			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTACTATTTCCTCCAATTATAGGAACGTTGTCGGGGAGTCTGGTAGTACTAGTATTTAA	PLTISSNYRNVVGESGSTSI*	-2.181	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22706	RGNSPQFLNSWPNRSASDIL			20	SLAY-screened peptide P1056			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGGGTAATTCTCCCCAGTTTCTCAATTCGTGGCCGAATCGTTCGGCTAGTGACATCCTCTAA	RGNSPQFLNSWPNRSASDIL*	-2.18	0.00051	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22707	NGQGTATPATYSNSGYNARC			20	SLAY-screened peptide P1057			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGGGCAGGGTACTGCTACGCCCGCGACTTACTCTAACTCTGGTTATAATGCTCGCTGCTAA	NGQGTATPATYSNSGYNARC*	-2.18	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22708	PTLLNNPQTGQSTNTSHEWQ			20	SLAY-screened peptide P1058			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACTCTGCTTAACAACCCTCAGACCGGTCAGAGCACTAATACTTCTCATGAGTGGCAGTAA	PTLLNNPQTGQSTNTSHEWQ*	-2.18	0.000847	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22709	MLYPIWQPINILMA			14	SLAY-screened peptide P1059			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTTGTACCCCATCTGGCAGCCGATCAATATTCTCATGGCTTAGTAGTCTCTTAGGTATTAA	MLYPIWQPINILMA**SLRY*	-2.179	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22710	SALANLAQPLTTLSMIRTRAN			21	SLAY-screened peptide P1060			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGCTCTCGCGAATCTGGCTCAGCCTCTTACTACGTTGTCCATGATACGAACCAGAGCTAAC	SALANLAQPLTTLSMIRTRAN	-2.179	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22711	SNATAINSLAYYLFDISVAA			20	SLAY-screened peptide P1061			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGAACGCCACGGCCATCAATTCCCTGGCGTATTATCTCTTTGATATTAGCGTGGCCGCTTAA	SNATAINSLAYYLFDISVAA*	-2.179	1.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22712	MHPWSNAHTLATDSSSADSH			20	SLAY-screened peptide P1062			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCACCCCTGGTCTAACGCTCATACGCTTGCGACGGACAGTTCCTCTGCTGATTCTCATTAA	MHPWSNAHTLATDSSSADSH*	-2.179	0.001379	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22713	IYCIVFPRLYDNCSIVGMSCN			21	SLAY-screened peptide P1063			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTACTGTATCGTGTTCCCTCGCTTGTACGATAACTGTTCTATCGTCGGCATGTCTTGTAAC	IYCIVFPRLYDNCSIVGMSCN	-2.178	0.000492	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22714	CYRIPLC			7	SLAY-screened peptide P1064			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTATCGTATCCCTCTGTGCTAGACTACGTCTGACTCGTATAATGGCTCCTCCTCGCCTTAA	CYRIPLC*TTSDSYNGSSSP*	-2.178	0.000362	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22715	LGREMFFSRYNGNHSRHTSS			20	SLAY-screened peptide P1065			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGGCAGGGAGATGTTTTTTTCCCGTTACAATGGCAATCACTCCCGGCATACCTCTTCCTAA	LGREMFFSRYNGNHSRHTSS*	-2.178	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22716	ICSRNMHMYRRIFNDYMFCD			20	SLAY-screened peptide P1066			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTGCTCGCGCAACATGCATATGTACCGCCGTATCTTCAATGATTACATGTTCTGTGATTAA	ICSRNMHMYRRIFNDYMFCD*	-2.177	0.000903	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22717	RSDHLPVRPIPSHILVTEDY			20	SLAY-screened peptide P1067			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAGCGATCACTTGCCTGTGCGCCCGATTCCTAGCCATATTTTGGTTACTGAGGACTATTAA	RSDHLPVRPIPSHILVTEDY*	-2.177	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22718	KHRLYTITLLDIPTSYNTLY			20	SLAY-screened peptide P1068			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCATCGTCTTTATACTATCACTCTGCTCGACATCCCGACGAGCTATAATACCCTCTACTAA	KHRLYTITLLDIPTSYNTLY*	-2.177	0.00129	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22719	CYPLSRSVCYFVTTYFLFSF			20	SLAY-screened peptide P1069			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTATCCTCTTTCTCGTTCTGTGTGCTATTTTGTCACCACCTATTTCCTCTTCAGTTTCTAA	CYPLSRSVCYFVTTYFLFSF*	-2.176	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22720	YGYDHSLPHRYYRALPVTLL			20	SLAY-screened peptide P1070			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGGCTATGACCACAGCCTCCCCCACCGGTACTACCGTGCCCTTCCGGTTACGCTCCTCTAA	YGYDHSLPHRYYRALPVTLL*	-2.176	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22721	GWPDSNLTMRNQSRIYCGT			19	SLAY-screened peptide P1071			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGTGGCCCGACAGTAATCTCACTATGCGTAACCAGAGCCGTATCTACTGTGGCACTTAACTG	GWPDSNLTMRNQSRIYCGT*L	-2.176	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22722	QTNYTVTSFSLVLTTMVMWH			20	SLAY-screened peptide P1072			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGACCAATTACACTGTCACGTCGTTTAGCCTTGTTCTCACCACGATGGTCATGTGGCACTAA	QTNYTVTSFSLVLTTMVMWH*	-2.175	5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22723	AGPENAAAPANFVDISLYAC			20	SLAY-screened peptide P1073			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGGGCCTGAGAATGCTGCTGCGCCGGCCAATTTTGTTGATATCTCTTTGTATGCTTGTTAA	AGPENAAAPANFVDISLYAC*	-2.174	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22724	TRIRIFGVCAFPATS			15	SLAY-screened peptide P1074			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCGTATTAGGATTTTTGGGGTTTGCGCGTTCCCGGCTACTAGTTAGGCGTCTCTTGCCTAA	TRIRIFGVCAFPATS*ASLA*	-2.173	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22725	RDFAYTPGNPFNPGLNSPRR			20	SLAY-screened peptide P1075			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGATTTTGCTTACACGCCCGGCAATCCTTTTAACCCCGGGCTCAACAGCCCTCGTCGTTAA	RDFAYTPGNPFNPGLNSPRR*	-2.172	0.003904	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22726	HNLCSIDNFYTIPSV			15	SLAY-screened peptide P1076			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAATCTCTGTTCCATTGATAATTTTTATACGATCCCTTCCGTCTAGGGGGGGCACTGTTAA	HNLCSIDNFYTIPSV*GGHC*	-2.172	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22727	HCDTTIKACSRYALR			15	SLAY-screened peptide P1077			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGTGATACGACGATTAAGGCTTGTAGTCGTTATGCTCTTCGTTAGGACCTGTTTGTCTAA	HCDTTIKACSRYALR*DLFV*	-2.171	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22728	NNILFTRFFPNDHGPRVGYH			20	SLAY-screened peptide P1078			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATAACATCCTCTTTACTCGTTTCTTTCCTAATGATCACGGGCCCAGGGTCGGCTACCACTAA	NNILFTRFFPNDHGPRVGYH*	-2.171	0.003973	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22729	AATVSNCLGLYYLSHNDTSFN			21	SLAY-screened peptide P1079			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGCTACTGTGTCTAATTGTCTGGGCCTTTACTATCTGAGTCACAATGACACTTCCTTTAAC	AATVSNCLGLYYLSHNDTSFN	-2.17	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22730	SFSGLCEDTETILKRRTT			18	SLAY-screened peptide P1080			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTTCTCTGGCCTTTGCGAGGACACGGAGACTATTCTCAAGAGGCGCACCACTTAGAATTAA	SFSGLCEDTETILKRRTT*N*	-2.17	0.000319	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22731	NRACSTPTSFCSVPQVPNTY			20	SLAY-screened peptide P1081			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACAGGGCCTGTAGCACCCCTACCAGCTTCTGCTCGGTCCCGCAGGTTCCGAACACTTATTAA	NRACSTPTSFCSVPQVPNTY*	-2.17	0.000344	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22732	CPREPRHDTVLLHHFTCRAH			20	SLAY-screened peptide P1082			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCCAGGGAGCCTAGGCATGATACGGTTTTGCTTCACCATTTCACCTGCAGGGCCCATTAA	CPREPRHDTVLLHHFTCRAH*	-2.17	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22733	PPLASCLAMRGFTITVPQLT			20	SLAY-screened peptide P1083			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCCCTGGCCTCGTGCCTCGCCATGCGTGGCTTTACTATTACTGTTCCGCAGTTGACCTAA	PPLASCLAMRGFTITVPQLT*	-2.169	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22734	AIFLVLFLFGHKSLFLIYRR			20	SLAY-screened peptide P1084			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCATCTTCCTTGTTTTGTTCCTGTTCGGCCATAAGAGCCTTTTCCTTATTTACCGGCGGTAA	AIFLVLFLFGHKSLFLIYRR*	-2.169	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22735	FSKEMMKSVHSMAQNALIVS			20	SLAY-screened peptide P1085			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTCGAAGGAGATGATGAAGAGTGTCCATTCCATGGCCCAGAATGCCCTGATTGTTAGCTAA	FSKEMMKSVHSMAQNALIVS*	-2.168	0.00015	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22736	GLPYDLPDVPNVH			13	SLAY-screened peptide P1086			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCTGCCCTACGATTTGCCTGACGTTCCGAATGTGCATTAGGCGCGCGGTGTTCGCACCTAA	GLPYDLPDVPNVH*ARGVRT*	-2.167	0.000174	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22737	RDTRCLPTHLLNSSHDPCTA			20	SLAY-screened peptide P1087			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGACACCCGTTGCTTGCCCACCCACCTTTTGAACAGTTCGCACGATCCTTGCACCGCGTAA	RDTRCLPTHLLNSSHDPCTA*	-2.166	0.000103	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22738	SRASYSNSHGTRHSHCTSSY			20	SLAY-screened peptide P1088			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCGTGCTTCTTACTCTAACTCCCATGGTACCAGGCATTCTCATTGCACCAGTTCCTACTAA	SRASYSNSHGTRHSHCTSSY*	-2.165	4.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22739	DPLRPNNPTSRFAVYSAIGT			20	SLAY-screened peptide P1089			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCCGCTTCGGCCTAATAATCCGACGTCTCGTTTTGCCGTGTACAGTGCTATTGGCACCTAA	DPLRPNNPTSRFAVYSAIGT*	-2.164	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22740	QYYVLHRRRLSY			12	SLAY-screened peptide P1090			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTACTATGTACTCCATCGACGTCGATTATCATATTGATGCCTAACTGAGTAAGTCGACCTG	QYYVLHRRRLSY*CLTE*VDL	-2.163	0.002885	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22741	AN			2	SLAY-screened peptide P1091			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTAATTAGCGCAACGCTTACCCTGAGGTCGTCTACCATATTAACCCCTTTACGCAGCAGTAA	AN*RNAYPEVVYHINPFTQQ*	-2.162	2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22742	CDRVSTCAAVGLLYFCKRFA			20	SLAY-screened peptide P1092			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGACAGGGTCTCCACCTGCGCGGCCGTCGGGCTTCTCTACTTTTGTAAGCGTTTTGCTTAA	CDRVSTCAAVGLLYFCKRFA*	-2.162	1.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22743	YRVRGPEKRTHVVLVSC			17	SLAY-screened peptide P1093			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCGTGTGAGGGGTCCTGAGAAGAGGACGCATGTTGTCCTTGTCTCGTGCTAGTATCCCTAA	YRVRGPEKRTHVVLVSC*YP*	-2.162	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22744	PCTYFDSLTRWFMLDAYPNL			20	SLAY-screened peptide P1094			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGTACGTACTTCGATAGTCTCACTCGTTGGTTCATGTTGGATGCTTACCCTAACTTGTAA	PCTYFDSLTRWFMLDAYPNL*	-2.162	0.003911	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22745	RY			2	SLAY-screened peptide P1095			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTACTAGGGCCCGATTTATTTCTGCGAGTGCCGTTCTAATTTCTGTCACTATTCGTCTTAA	RY*GPIYFCECRSNFCHYSS*	-2.161	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22746	PRSSDNQCQILHCNSLYNNY			20	SLAY-screened peptide P1096			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGCTCGTCCGACAATCAGTGCCAGATTCTTCACTGTAATAGCCTGTACAATAATTATTAA	PRSSDNQCQILHCNSLYNNY*	-2.161	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22747	FPYNPRCYWPSYHPVDNYSV			20	SLAY-screened peptide P1097			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCCTTACAATCCTAGGTGTTATTGGCCCTCCTACCACCCTGTTGATAATTATAGCGTTTAA	FPYNPRCYWPSYHPVDNYSV*	-2.16	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22748	TPPSPSNTPKYVQVTMAYHL			20	SLAY-screened peptide P1098			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCCGCCCAGCCCGAGTAATACTCCCAAGTACGTGCAGGTTACTATGGCCTACCATCTCTAA	TPPSPSNTPKYVQVTMAYHL*	-2.159	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22749	WPSNLVRRFFQPTMLHHSAV			20	SLAY-screened peptide P1099			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCCCTCCAACTTGGTGCGCAGGTTCTTTCAGCCCACTATGCTCCATCACTCGGCCGTGTAA	WPSNLVRRFFQPTMLHHSAV*	-2.159	3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22750	AHEQVVNSNCPNNDLPCP			18	SLAY-screened peptide P1100			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCATGAGCAGGTGGTCAATTCCAATTGCCCCAATAATGACCTTCCGTGCCCTTAGGTCTAA	AHEQVVNSNCPNNDLPCP*V*	-2.159	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22751	ASSSHPILAFNTTLDRINDL			20	SLAY-screened peptide P1101			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAGCAGTAGTCACCCTATCCTGGCTTTTAATACTACCCTGGATCGGATCAATGACCTCTAA	ASSSHPILAFNTTLDRINDL*	-2.158	4.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22752	LPPVPLLFLRHFRVCYSHLP			20	SLAY-screened peptide P1102			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCCCCCGTGCCGTTGCTCTTCCTTCGCCACTTCCGCGTCTGTTACTCTCATCTTCCCTAA	LPPVPLLFLRHFRVCYSHLP*	-2.156	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22753	PGSLNAAGGLLSVTTYPYDY			20	SLAY-screened peptide P1103			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGGGTCGCTCAATGCCGCCGGTGGGCTCCTCTCTGTCACTACTTATCCGTACGATTATTAA	PGSLNAAGGLLSVTTYPYDY*	-2.155	0.000391	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22754	PLGTPPRFHLRISTLIRSTG			20	SLAY-screened peptide P1104			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTGGGACCCCTCCCCGGTTTCATCTGCGGATCTCCACGCTCATCAGGTCCACTGGCTAA	PLGTPPRFHLRISTLIRSTG*	-2.154	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22755	PSPWGGSSLAL			11	SLAY-screened peptide P1105			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTCCCCCTGGGGCGGGAGCTCCCTCGCTCTGTAGCCGTAGAGTCATCAGACTGCTTTGTAA	PSPWGGSSLAL*P*SHQTAL*	-2.153	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22756	LPHTE			5	SLAY-screened peptide P1106			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCCCATACCGAGTAGAGTGCTCGTTTGACGCGGGATGTGTTTGCCTACGGTATTGCCTAA	LPHTE*SARLTRDVFAYGIA*	-2.153	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22757	ERHPFHVHVFRFWAMNSRLR			20	SLAY-screened peptide P1107			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGAGGCACCCTTTTCATGTTCACGTTTTCCGTTTCTGGGCTATGAATAGCAGGCTCCGTTAA	ERHPFHVHVFRFWAMNSRLR*	-2.153	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22758	CLVLSTCCPNEHRSY			15	SLAY-screened peptide P1108			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTCGTTCTGAGCACCTGTTGCCCCAACGAGCACCGTTCTTATTAGCAGTTGAACCTGTAA	CLVLSTCCPNEHRSY*QLNL*	-2.152	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22759	FARAVVSEHAHYHCYQNIAP			20	SLAY-screened peptide P1109			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGCTCGCGCCGTCGTTTCGGAGCATGCCCATTATCACTGTTATCAGAATATTGCGCCCTAA	FARAVVSEHAHYHCYQNIAP*	-2.152	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22760	PWNTNLH			7	SLAY-screened peptide P1110			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGGAATACTAATCTTCATTAGCATTGTACCTGCTCTAGCAATATCCCTCAGGATCACTAA	PWNTNLH*HCTCSSNIPQDH*	-2.152	0.002013	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22761	HPDYLLAGNRTKSPKDNAQR			20	SLAY-screened peptide P1111			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCTGATTACCTCTTGGCGGGTAACCGTACTAAGTCCCCCAAGGACAACGCCCAGCGTTAA	HPDYLLAGNRTKSPKDNAQR*	-2.152	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22762	IRDNIPTACSHCTNGTDQVT			20	SLAY-screened peptide P1112			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCGCGATAATATCCCGACCGCGTGTTCGCATTGCACTAATGGTACGGATCAGGTCACCTAA	IRDNIPTACSHCTNGTDQVT*	-2.151	0.000784	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22763	PNGFNTPSFTIRSVYRLVFA			20	SLAY-screened peptide P1113			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAACGGGTTCAATACCCCCAGTTTTACTATTCGTTCCGTTTATAGGCTGGTCTTTGCTTAA	PNGFNTPSFTIRSVYRLVFA*	-2.151	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22764	LLHVPTPLLMQKLLFRHLYH			20	SLAY-screened peptide P1114			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCTCCATGTGCCCACCCCCCTTCTTATGCAGAAGCTCTTGTTTAGGCATCTCTATCACTAA	LLHVPTPLLMQKLLFRHLYH*	-2.151	0.000555	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22765	DLLDAGSHH			9	SLAY-screened peptide P1115			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTTGCTCGACGCTGGGTCCCATCACTAGCACCTTGGGTACAGCTTGACTTGGTCTTTTTAA	DLLDAGSHH*HLGYSLTWSF*	-2.15	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22766	AQSFAFLFLRKMALEPRQLI			20	SLAY-screened peptide P1116			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCAGTCTTTTGCGTTTCTGTTTCTCCGCAAGATGGCTCTGGAGCCGCGGCAGCTTATCTAA	AQSFAFLFLRKMALEPRQLI*	-2.15	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22767	ATIYKWPLDHYTHANSSNYR			20	SLAY-screened peptide P1117			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTACCATTTATAAGTGGCCTTTGGACCATTACACGCATGCTAATTCTTCGAATTATAGGTAA	ATIYKWPLDHYTHANSSNYR*	-2.149	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22768	CGFGLPSFRCTDHFRYVIYD			20	SLAY-screened peptide P1118			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGGCTTTGGGCTCCCCTCCTTTAGGTGCACGGACCACTTCCGCTATGTGATTTACGATTAA	CGFGLPSFRCTDHFRYVIYD*	-2.149	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22769	RTPPDSPFAILIFWRRSRCVN			21	SLAY-screened peptide P1119			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGACCCCTCCGGATTCTCCGTTTGCCATACTGATATTCTGGCGTCGCAGCAGATGCGTTAAC	RTPPDSPFAILIFWRRSRCVN	-2.149	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22770	YSDIVKFNMLKDPQSDSVAS			20	SLAY-screened peptide P1120			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTCCGATATTGTGAAGTTTAATATGCTTAAGGACCCCCAGAGCGACTCCGTCGCCTCCTAA	YSDIVKFNMLKDPQSDSVAS*	-2.148	0.000506	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22771	NTLDYNYPLIHLRDYVKTLC			20	SLAY-screened peptide P1121			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACACCCTGGATTATAATTACCCGTTGATTCATCTGCGCGATTACGTTAAGACGCTTTGTTAA	NTLDYNYPLIHLRDYVKTLC*	-2.148	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22772	NLLSQVYAPRPLGLLSRLKS			20	SLAY-screened peptide P1122			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCTGCTTTCCCAGGTTTATGCTCCTCGGCCTCTTGGCTTGCTCAGCCGTCTTAAGTCTTAA	NLLSQVYAPRPLGLLSRLKS*	-2.147	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22773	MYSNSLRTCAYSDTHSASVD			20	SLAY-screened peptide P1123			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTACTCTAATTCTCTCCGGACGTGTGCCTATAGCGATACTCACAGTGCCAGCGTGGACTAA	MYSNSLRTCAYSDTHSASVD*	-2.147	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22774	PAPYRTYAHLSGRCLNQYTR			20	SLAY-screened peptide P1124			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCCCCCTATCGTACCTACGCTCACTTGTCGGGTCGTTGTTTGAACCAGTATACTCGCTAA	PAPYRTYAHLSGRCLNQYTR*	-2.147	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22775	CDYTLPTTAPTRPPSAPTWR			20	SLAY-screened peptide P1125			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGACTATACGCTTCCCACTACTGCTCCGACCCGTCCGCCCTCGGCGCCTACTTGGCGCTAA	CDYTLPTTAPTRPPSAPTWR*	-2.146	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22776	ASSTRHGSEPHGSIHSPYCY			20	SLAY-screened peptide P1126			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTAGCTCTACCAGGCATGGGTCCGAGCCCCACGGGAGTATCCATAGTCCTTATTGTTATTAA	ASSTRHGSEPHGSIHSPYCY*	-2.146	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22777	GEGELIRVSKHCHLDAIMSG			20	SLAY-screened peptide P1127			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGGAGGGGGAGCTCATCAGGGTCTCTAAGCACTGTCATCTTGATGCTATCATGTCCGGCTAA	GEGELIRVSKHCHLDAIMSG*	-2.146	0.00014	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22778	HRRCASVSLAPRNALPTDHL			20	SLAY-screened peptide P1128			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCGCCGCTGCGCCTCGGTCTCGCTCGCCCCGCGGAACGCCCTGCCGACTGATCACTTGTAA	HRRCASVSLAPRNALPTDHL*	-2.146	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22779	SIKFRSTRYLVRGVSTLFDF			20	SLAY-screened peptide P1129			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCATTAAGTTTCGGAGTACCCGTTATCTCGTTCGCGGTGTCAGCACCTTGTTTGACTTTTAA	SIKFRSTRYLVRGVSTLFDF*	-2.144	2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22780	TLIYITWTSSPRLMNIITAN			20	SLAY-screened peptide P1130			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCTGATCTATATTACTTGGACCTCGTCCCCTCGTTTGATGAACATTATTACCGCGAATTAA	TLIYITWTSSPRLMNIITAN*	-2.144	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22781	GFAHYLL			7	SLAY-screened peptide P1131			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTTCGCGCACTACCTCCTCTAGTGTCTTCACCACGACTATGGCGACGCGAATAACGATTAA	GFAHYLL*CLHHDYGDANND*	-2.143	0.00019	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22782	LGPAFVGRHPRCPQFFDWSR			20	SLAY-screened peptide P1132			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGGGCCCGCCTTTGTGGGTCGTCATCCGCGTTGTCCGCAGTTTTTTGATTGGTCTCGCTAA	LGPAFVGRHPRCPQFFDWSR*	-2.143	0.000636	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22783	PPTACFAEPLSNYSFPYVLL			20	SLAY-screened peptide P1133			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCGACTGCCTGCTTCGCGGAGCCGCTTTCCAACTATTCCTTCCCTTATGTGCTGCTCTAA	PPTACFAEPLSNYSFPYVLL*	-2.143	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22784	PRTVFVI			7	SLAY-screened peptide P1134			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGTACTGTCTTTGTCATCTAGCTCATTGTGCCGGATCATAATATTGATGGTAATACGTAA	PRTVFVI*LIVPDHNIDGNT*	-2.142	0.003074	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22785	GAYAFHHWLFSYLVYMRRMN			20	SLAY-screened peptide P1135			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGGCGTATGCTTTTCACCACTGGCTCTTTAGCTACTTGGTCTATATGAGGAGGATGAACTAA	GAYAFHHWLFSYLVYMRRMN*	-2.142	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22786	HFVPVYSNLFFLTFPRVIDT			20	SLAY-screened peptide P1136			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTTTGTTCCGGTTTATTCCAACCTTTTCTTCCTTACTTTCCCTAGGGTTATTGATACCTAA	HFVPVYSNLFFLTFPRVIDT*	-2.14	2.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22787	PSAELCMPNAFPGLPNGSSP			20	SLAY-screened peptide P1137			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCTGCGGAGCTTTGCATGCCCAATGCTTTCCCTGGTCTCCCTAACGGCTCTAGTCCGTAA	PSAELCMPNAFPGLPNGSSP*	-2.139	0.001087	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22788	STLVLIRTCHSAISTYYCPS			20	SLAY-screened peptide P1138			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTACCCTGGTCCTTATTAGGACCTGCCATTCGGCTATCTCTACTTACTATTGTCCCTCCTAA	STLVLIRTCHSAISTYYCPS*	-2.138	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22789	THECTLTLVQHDI			13	SLAY-screened peptide P1139			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCACGAGTGCACCCTCACGCTCGTGCAGCACGATATCTAGGCGAGTCAGCTGATCACGTAA	THECTLTLVQHDI*ASQLIT*	-2.138	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22790	WLVPSRRLSAYCVLIFSRSD			20	SLAY-screened peptide P1140			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCTTGTCCCGAGTCGCCGGCTGTCGGCGTATTGCGTTCTTATTTTTTCTCGTTCTGATTAA	WLVPSRRLSAYCVLIFSRSD*	-2.137	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22791	TCAVASRIRSSYKYCTNTTH			20	SLAY-screened peptide P1141			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTGTGCGGTTGCGTCGCGTATTCGCTCTTCGTATAAGTACTGTACGAACACCACCCATTAA	TCAVASRIRSSYKYCTNTTH*	-2.137	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22792	RSYTEETYLSYFVYTNRCDRY			21	SLAY-screened peptide P1142			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCCTACACGGAGGAGACTTACCTCTCTTACTTTGTTTATACGAACCGGTGCGATCGGTAC	RSYTEETYLSYFVYTNRCDRY	-2.137	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22793	FTSQALSADISDDTCWAHAQ			20	SLAY-screened peptide P1143			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTACTTCTCAGGCGCTTTCCGCCGACATCAGTGACGATACCTGTTGGGCGCATGCCCAGTAA	FTSQALSADISDDTCWAHAQ*	-2.137	2.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22794	TPCNFSHQSCHGLSTGLLTD			20	SLAY-screened peptide P1144			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCCGTGTAACTTCAGCCACCAGTCTTGCCACGGCCTTTCTACTGGCCTGCTTACTGATTAA	TPCNFSHQSCHGLSTGLLTD*	-2.136	0.001118	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22795	ASPHFCPGSRHSAIPPYMLL			20	SLAY-screened peptide P1145			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTCCCCTCATTTCTGTCCTGGTTCGAGGCACTCTGCTATTCCGCCCTACATGTTGCTTTAA	ASPHFCPGSRHSAIPPYMLL*	-2.136	1.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22796	SGWFS			5	SLAY-screened peptide P1146			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGGCTGGTTTAGTTAGCAGCCCCGTGGCGCGTCGCTCAACGAGGCGAATTCTCTTACTTAA	SGWFS*QPRGASLNEANSLT*	-2.136	0.000744	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22797	TSSPLSLCSSSQALATPSAN			20	SLAY-screened peptide P1147			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTCTTCTCCTTTGTCGCTGTGTTCCAGTAGTCAGGCCTTGGCGACTCCTTCGGCGAACTAA	TSSPLSLCSSSQALATPSAN*	-2.136	9.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22798	CI			2	SLAY-screened peptide P1148			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTATCTAGCCTCATCTTGAGCGCCAGCATAAGTGCTTCTCCCGTCATATTATGATGCATTAA	CI*PHLERQHKCFSRHIMMH*	-2.135	0.000173	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22799	WPPCGSHCLF			10	SLAY-screened peptide P1149			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCCCCCTTGTGGCAGTCACTGTTTGTTCTAGAGTTATTCTGATAACGATCCTGACGCGTAA	WPPCGSHCLF*SYSDNDPDA*	-2.135	0.000535	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22800	RVHKLLPLHSGLSSDSM			17	SLAY-screened peptide P1150			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGTCCATAAGCTGTTGCCGCTCCACAGTGGCCTGTCCAGTGACTCCATGTAGCAGTTCTAA	RVHKLLPLHSGLSSDSM*QF*	-2.135	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22801	LAAPNQTTEGRDIPNWAHPT			20	SLAY-screened peptide P1151			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGCCGCCCCGAACCAGACTACGGAGGGCCGTGATATCCCGAACTGGGCTCATCCCACTTAA	LAAPNQTTEGRDIPNWAHPT*	-2.135	0.00384	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22802	AALGSWNSTTYSPLVTPMNR			20	SLAY-screened peptide P1152			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCTCTTGGTTCTTGGAACTCTACGACGTATTCTCCTCTGGTTACTCCTATGAATCGCTAA	AALGSWNSTTYSPLVTPMNR*	-2.134	0.00026	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22803	LCSPLYDTF			9	SLAY-screened peptide P1153			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTGTAGTCCTCTTTATGACACGTTTTAGGCCATTTTTTAGGCGTTTAACACCGCCGATTAA	LCSPLYDTF*AIF*AFNTAD*	-2.133	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22804	DNSFGDRCGHYSYHVCISSA			20	SLAY-screened peptide P1154			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATAATTCGTTTGGCGACAGGTGCGGCCATTATAGCTATCATGTGTGTATCTCTTCGGCGTAA	DNSFGDRCGHYSYHVCISSA*	-2.132	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22805	CLYSHIGVPPSLPTDFYYSR			20	SLAY-screened peptide P1155			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTTGTATTCTCATATTGGCGTGCCGCCCTCGCTTCCGACGGATTTCTACTATAGCAGGTAA	CLYSHIGVPPSLPTDFYYSR*	-2.132	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22806	LIFYFLRVWPDLPYYCTDSD			20	SLAY-screened peptide P1156			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGATTTTCTACTTTCTGAGGGTCTGGCCTGACCTGCCCTATTACTGTACCGACTCCGATTAA	LIFYFLRVWPDLPYYCTDSD*	-2.131	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22807	HGIPYYRTVSISDTLAT			17	SLAY-screened peptide P1157			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGGTATCCCTTATTATCGGACTGTTAGCATTAGCGACACCTTGGCGACTTAGTGCTAAGTA	HGIPYYRTVSISDTLAT*C*V	-2.131	0.002641	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22808	PQNYL			5	SLAY-screened peptide P1158			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCAGAACTACCTGTAGGCTCCGCTTTCGCGCGCTGCTAACCATCACTCCTACACGTCTTAA	PQNYL*APLSRAANHHSYTS*	-2.131	0.000297	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22809	TGLIRWYQSCRHLPFRRLY			19	SLAY-screened peptide P1159			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGGGCTGATTAGGTGGTACCAGTCGTGCCGGCACTTGCCGTTCAGGCGCTTGTACTAGTAA	TGLIRWYQSCRHLPFRRLY**	-2.13	0.000575	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22810	HLFILNKTHVDCRAH			15	SLAY-screened peptide P1160			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCTTTTTATTCTTAATAAGACTCACGTCGATTGCAGGGCTCATTAGACTATGCACTGTTAA	HLFILNKTHVDCRAH*TMHC*	-2.129	3.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22811	RVIRRSGGTI			10	SLAY-screened peptide P1161			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGTCATCCGGCGATCCGGAGGAACAATATGAATAAGTCTCATATTATCCAGGGCCTTTAAC	RVIRRSGGTI*ISLILSRAFN	-2.128	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22812	PELGPPPTIGPRF			13	SLAY-screened peptide P1162			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGAGCTTGGGCCTCCTCCGACTATTGGCCCTCGTTTCTAGAGCCTTTTGGCGCCCGCGTAA	PELGPPPTIGPRF*SLLAPA*	-2.128	0.000173	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22813	FSTFHMRMYSPEYEPANYHP			20	SLAY-screened peptide P1163			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTCTACCTTTCATATGCGGATGTATTCGCCCGAGTATGAGCCTGCGAACTACCACCCGTAA	FSTFHMRMYSPEYEPANYHP*	-2.128	0.00104	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22814	YNGVLNGCTRRNIKP			15	SLAY-screened peptide P1164			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACAACGGCGTCCTGAATGGTTGCACCCGTAGGAACATCAAGCCTTAGAAGGCGGCCGCTTAA	YNGVLNGCTRRNIKP*KAAA*	-2.128	0.003781	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22815	LYLVAGAILRIYHCRLDGSY			20	SLAY-screened peptide P1165			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTATCTCGTGGCGGGTGCTATCCTTCGTATTTATCATTGCAGGCTGGACGGTAGTTATTAA	LYLVAGAILRIYHCRLDGSY*	-2.127	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22816	FPNPMDLSSPWYVLHGQKVP			20	SLAY-screened peptide P1166			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCCCAATCCTATGGATTTGTCCTCCCCTTGGTATGTCCTCCACGGGCAGAAGGTGCCTTAA	FPNPMDLSSPWYVLHGQKVP*	-2.127	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22817	RLLLLLLLRSLYDPKARKPR			20	SLAY-screened peptide P1167			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTTGCTCTTGTTGCTTCTTCTGCGTAGTCTCTATGACCCCAAGGCTCGTAAGCCTCGTTAA	RLLLLLLLRSLYDPKARKPR*	-2.127	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22818	NCGYRTPAPGTSRPA			15	SLAY-screened peptide P1168			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTGCGGGTATCGCACTCCGGCTCCGGGCACTTCCCGTCCGGCTTAGGCGCGCCATCTGTAA	NCGYRTPAPGTSRPA*ARHL*	-2.126	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22819	SSTRSARAQDPMALSHTHTG			20	SLAY-screened peptide P1169			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTCGACTAGGTCGGCCCGGGCCCAGGATCCTATGGCTTTGAGCCATACCCATACTGGCTAA	SSTRSARAQDPMALSHTHTG*	-2.126	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22820	LPSSDCYITPDSATFLIINI			20	SLAY-screened peptide P1170			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCTAGCTCTGACTGTTACATTACGCCTGATTCCGCTACTTTTCTGATTATCAATATTTAA	LPSSDCYITPDSATFLIINI*	-2.125	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22821	DYNAFSEETKVYCIYLIHNT			20	SLAY-screened peptide P1171			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTACAACGCTTTCTCCGAGGAGACGAAGGTGTATTGTATTTACCTTATCCACAATACTTAA	DYNAFSEETKVYCIYLIHNT*	-2.124	0.000804	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22822	HYDWWNVPCHSRYSPSYVLL			20	SLAY-screened peptide P1172			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTACGACTGGTGGAATGTTCCGTGTCATTCTCGTTATTCCCCGAGTTACGTTCTGCTCTAA	HYDWWNVPCHSRYSPSYVLL*	-2.124	0.000998	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22823	IDLWSFHINGNLASTAASISN			21	SLAY-screened peptide P1173			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGATCTTTGGAGCTTTCACATTAACGGCAACCTTGCGTCCACCGCCGCATCCATATCTAAC	IDLWSFHINGNLASTAASISN	-2.123	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22824	NADCTRHSIRNDQC			14	SLAY-screened peptide P1174			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGCGGACTGCACGCGTCATAGCATCCGCAATGACCAGTGCTAGAATGCCTAGGGTATTTAA	NADCTRHSIRNDQC*NA*GI*	-2.123	3.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22825	AQHVIPCQIPYYLPITYVLR			20	SLAY-screened peptide P1175			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCAGCATGTGATCCCTTGCCAGATCCCGTACTATCTCCCGATTACGTATGTGCTCCGTTAA	AQHVIPCQIPYYLPITYVLR*	-2.123	6.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22826	STYYTLPNVPICPYSHWHPY			20	SLAY-screened peptide P1176			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCACGTATTATACGCTTCCCAACGTGCCTATCTGTCCGTATTCGCATTGGCATCCCTATTAA	STYYTLPNVPICPYSHWHPY*	-2.123	0.002088	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22827	LPAGYETRRVYLANSAGGTS			20	SLAY-screened peptide P1177			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCTGCGGGGTACGAGACTCGTCGGGTCTATTTGGCCAATAGTGCTGGTGGGACGTCCTAA	LPAGYETRRVYLANSAGGTS*	-2.122	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22828	NPRLDLTRSSSQFSVSPGRR			20	SLAY-screened peptide P1178			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCCCCGCCTTGATCTGACCCGGAGCTCTTCGCAGTTTTCGGTTTCCCCTGGTCGTAGGTAA	NPRLDLTRSSSQFSVSPGRR*	-2.122	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22829	HKGAYRVSSLIITTMKMSQR			20	SLAY-screened peptide P1179			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAAGGGCGCCTACCGTGTTAGTAGTCTCATCATCACTACTATGAAGATGTCGCAGCGCTAA	HKGAYRVSSLIITTMKMSQR*	-2.121	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22830	SLALPIFTFTTHIAPHVTHL			20	SLAY-screened peptide P1180			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCTCGCCCTCCCGATTTTCACGTTTACTACTCATATTGCGCCTCATGTCACGCATCTTTAA	SLALPIFTFTTHIAPHVTHL*	-2.12	0.002071	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22831	LQYVPLTRSRHTLDSENTAW			20	SLAY-screened peptide P1181			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCAGTATGTCCCCCTGACGCGCAGTCGGCATACGCTTGATTCCGAGAACACCGCTTGGTAA	LQYVPLTRSRHTLDSENTAW*	-2.118	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22832	PRRNPVRYVMSKIQVIFLRN			20	SLAY-screened peptide P1182			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGGCGGAACCCCGTGCGGTATGTTATGAGTAAGATTCAGGTCATCTTCCTCCGTAATTAA	PRRNPVRYVMSKIQVIFLRN*	-2.118	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22833	RAGCIPLPPVLVYPNIHPST			20	SLAY-screened peptide P1183			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCGGGCTGTATCCCCCTGCCCCCTGTCCTGGTTTACCCGAACATTCACCCGAGCACTTAA	RAGCIPLPPVLVYPNIHPST*	-2.118	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22834	SFHAFATDVDAHSSLPGENT			20	SLAY-screened peptide P1184			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTTTCATGCGTTTGCGACGGACGTGGACGCGCATTCGTCCCTTCCTGGTGAGAACACCTAA	SFHAFATDVDAHSSLPGENT*	-2.117	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22835	YA			2	SLAY-screened peptide P1185			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGCGTAGGCCCAGCTTACCACCAGTAATGCTGGGCCCATTGGGGACTCTACTTCTTCGTAA	YA*AQLTTSNAGPIGDSTSS*	-2.117	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22836	NLRVKYSLPCTLVANALHHS			20	SLAY-screened peptide P1186			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCTCCGTGTCAAGTATTCGTTGCCTTGTACTTTGGTCGCCAATGCTCTCCACCATAGTTAA	NLRVKYSLPCTLVANALHHS*	-2.117	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22837	QRPLREARIKPNHHRGFHNL			20	SLAY-screened peptide P1187			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCGCCCGCTCCGCGAGGCGCGTATTAAGCCGAACCATCATCGGGGTTTTCATAATTTGTAA	QRPLREARIKPNHHRGFHNL*	-2.117	9.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22838	GEHPSVVTIPNNCYCYRCTD			20	SLAY-screened peptide P1188			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTGAGCATCCTTCTGTGGTTACGATTCCTAATAATTGTTACTGCTATAGGTGTACCGATTAA	GEHPSVVTIPNNCYCYRCTD*	-2.117	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22839	PPGQHNKAPDLEYTNPFRNL			20	SLAY-screened peptide P1189			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCGGGTCAGCACAATAAGGCCCCCGACCTGGAGTATACCAATCCTTTTCGTAACCTGTAA	PPGQHNKAPDLEYTNPFRNL*	-2.116	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22840	APLWPHQGLSRFSILFITITN			21	SLAY-screened peptide P1190			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCCTCTTTGGCCTCATCAGGGACTAAGTAGGTTTTCGATACTGTTCATCACCATAACTAAC	APLWPHQGLSRFSILFITITN	-2.116	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22841	DRCPSYHMFNPTVTYICRLG			20	SLAY-screened peptide P1191			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCGTTGTCCTTCGTATCACATGTTCAACCCGACGGTCACCTACATTTGCCGCCTTGGGTAA	DRCPSYHMFNPTVTYICRLG*	-2.116	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22842	PCQNPTSCHHSRSLSRQA			18	SLAY-screened peptide P1192			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGCCAGAACCCCACGAGTTGTCATCATTCCCGTTCGCTTTCGCGCCAGGCCTAGCAGTAA	PCQNPTSCHHSRSLSRQA*Q*	-2.116	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22843	GNYIWPILSDHIAN			14	SLAY-screened peptide P1193			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTAATTATATTTGGCCGATCCTTTCTGACCATATCGCTAACTAGTGCCTGCTCATTTCTTAA	GNYIWPILSDHIAN*CLLIS*	-2.116	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22844	PLKGLLVGHRASNDNSNNGR			20	SLAY-screened peptide P1194			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTCAAGGGGTTGCTGGTTGGCCACCGCGCGAGCAATGATAACAGTAATAACGGGAGGTAA	PLKGLLVGHRASNDNSNNGR*	-2.115	0.000739	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22845	VYVCTASDCFMSYT			14	SLAY-screened peptide P1195			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTACGTTTGCACTGCTTCGGATTGTTTCATGTCCTACACCTAGCCCGCGCAAGGAGGTAAC	VYVCTASDCFMSYT*PAQGGN	-2.115	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22846	CWSSP			5	SLAY-screened peptide P1196			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTGGTCGAGTCCGTAGATCAAGAACAGTCCCACGTATGCGGTTACCCATGTTCCGGCTTAA	CWSSP*IKNSPTYAVTHVPA*	-2.115	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22847	RKDIVCYYVSSHTYNALDNF			20	SLAY-screened peptide P1197			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAAGGATATCGTGTGCTACTACGTTAGTAGTCACACGTACAATGCTCTTGATAATTTTTAA	RKDIVCYYVSSHTYNALDNF*	-2.114	0.005743	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22848	LYTPNRPPTSLRSRTQTVHV			20	SLAY-screened peptide P1198			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTATACCCCCAACCGCCCTCCGACCAGCTTGCGTTCCCGCACGCAGACCGTGCATGTGTAA	LYTPNRPPTSLRSRTQTVHV*	-2.114	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22849	LLTTPNSHPHYRQ			13	SLAY-screened peptide P1199			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCTCACGACCCCTAACTCGCACCCGCACTATCGCCAGTAGCCTCTTATCTTCACTAGCTAA	LLTTPNSHPHYRQ*PLIFTS*	-2.114	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22850	TRLPPSLVSSLAISGTYAYG			20	SLAY-screened peptide P1200			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCGCCTGCCTCCTAGCCTCGTGTCTAGCCTTGCTATTTCGGGGACCTACGCTTACGGCTAA	TRLPPSLVSSLAISGTYAYG*	-2.113	0.000334	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22851	RHESHTHYTSSQVVYVRWLI			20	SLAY-screened peptide P1201			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCACGAGAGCCATACCCATTATACTTCTTCCCAGGTCGTGTATGTGAGGTGGCTTATCTAA	RHESHTHYTSSQVVYVRWLI*	-2.113	0.003611	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22852	RYNLLRLGPSRDKSIGIDPS			20	SLAY-screened peptide P1202			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTATAACCTGCTCAGGCTCGGGCCTTCTCGTGATAAGTCTATTGGTATTGATCCCAGTTAA	RYNLLRLGPSRDKSIGIDPS*	-2.113	0.001093	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22853	GGFHNRICKNY			11	SLAY-screened peptide P1203			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGGCTTCCATAATCGCATTTGTAAGAATTATTAGCGGTGTCGCATTTACTTTGACTACTAA	GGFHNRICKNY*RCRIYFDY*	-2.113	0.000141	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22854	CPYLYDLAYTTSLTLHLTPT			20	SLAY-screened peptide P1204			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCCTACCTGTATGATTTGGCCTACACCACTTCGCTCACGCTCCACCTTACTCCTACGTAA	CPYLYDLAYTTSLTLHLTPT*	-2.111	8.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22855	PSNLSPTANSLSSNCPITNS			20	SLAY-screened peptide P1205			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGTAACCTTAGTCCTACCGCTAATTCCTTGTCTAGTAATTGCCCCATTACCAACTCTTAA	PSNLSPTANSLSSNCPITNS*	-2.111	0.000186	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22856	TNPSDCISLVSTESRVFLLH			20	SLAY-screened peptide P1206			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAACCCTTCGGATTGTATTAGCCTGGTGTCGACCGAGTCGCGGGTTTTCCTTCTTCATTAA	TNPSDCISLVSTESRVFLLH*	-2.11	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22857	RCRFFCVTRVIYNAAGIKAL			20	SLAY-screened peptide P1207			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTGTAGGTTCTTTTGCGTGACGAGGGTCATCTACAATGCTGCCGGCATTAAGGCTCTGTAA	RCRFFCVTRVIYNAAGIKAL*	-2.11	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22858	YLPNELASSSNSNYTGFYCA			20	SLAY-screened peptide P1208			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCTGCCTAATGAGCTGGCCTCTAGTTCCAACAGCAATTATACCGGTTTTTATTGTGCCTAA	YLPNELASSSNSNYTGFYCA*	-2.109	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22859	VTFLRSGRPYMCMSS			15	SLAY-screened peptide P1209			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCACGTTCCTGCGTAGTGGTCGCCCTTACATGTGTATGTCGTCCTAGGAGGCGTGGACCTAA	VTFLRSGRPYMCMSS*EAWT*	-2.109	0.001013	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22860	GSYGLPNGLIPHSSIRIDYL			20	SLAY-screened peptide P1210			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTCCTACGGCCTTCCTAACGGTCTCATTCCGCATTCTTCGATCCGCATTGACTATCTTTAA	GSYGLPNGLIPHSSIRIDYL*	-2.109	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22861	PPATWVPPNTYPTPLVYIPH			20	SLAY-screened peptide P1211			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCGGCCACTTGGGTCCCTCCTAACACGTACCCCACGCCCCTGGTTTATATTCCTCATTAA	PPATWVPPNTYPTPLVYIPH*	-2.108	2.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22862	INRNVDKMHNLNLVPFLTCF			20	SLAY-screened peptide P1212			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAACCGTAATGTGGATAAGATGCACAATCTCAACCTGGTGCCCTTCCTTACTTGCTTTTAA	INRNVDKMHNLNLVPFLTCF*	-2.108	0.000555	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22863	DL			2	SLAY-screened peptide P1213			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCTCTAGCATGACAGTAGCTCCTTTTTCAACACCTATGACACTAATACTGACGCCTACTAA	DL*HDSSSFFNTYDTNTDAY*	-2.107	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22864	IHRHHHGSNSWTYLCAKSNL			20	SLAY-screened peptide P1214			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCATCGGCATCACCACGGTAGTAATTCGTGGACGTATCTTTGCGCCAAGAGCAACTTGTAA	IHRHHHGSNSWTYLCAKSNL*	-2.107	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22865	QPTQLMYTHDTDNRSCLGLT			20	SLAY-screened peptide P1215			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCGACTCAGTTGATGTATACTCATGACACGGATAATCGCAGCTGTCTGGGGCTCACTTAA	QPTQLMYTHDTDNRSCLGLT*	-2.106	0.000548	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22866	DPLHVRDCRILSHNMSNMTY			20	SLAY-screened peptide P1216			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCCGCTTCATGTGCGTGACTGTCGTATCCTCAGCCACAACATGAGTAATATGACTTATTAA	DPLHVRDCRILSHNMSNMTY*	-2.106	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22867	HFNCPLPTSRDPFESKESFR			20	SLAY-screened peptide P1217			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTTTAATTGCCCTCTTCCTACGAGCCGTGATCCTTTCGAGTCTAAGGAGAGTTTTCGTTAA	HFNCPLPTSRDPFESKESFR*	-2.106	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22868	LYTPFGYLLWSYSLASRDRK			20	SLAY-screened peptide P1218			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTACACGCCTTTCGGGTATTTGCTTTGGAGTTACTCGCTGGCTAGTCGCGATCGCAAGTAA	LYTPFGYLLWSYSLASRDRK*	-2.106	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22869	TSLLRHAPHSHRDPRHANTK			20	SLAY-screened peptide P1219			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTCCCTGCTTCGTCACGCGCCGCACAGTCACCGTGATCCTAGGCACGCTAATACGAAGTAA	TSLLRHAPHSHRDPRHANTK*	-2.105	0.00015	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22870	NSVLWILSPICIRGPSRRTVA			21	SLAY-screened peptide P1220			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATAGCGTGCTCTGGATCTTGTCGCCCATTTGCATTCGAGGTCCTAGTCGGCGCACTGTTGCT	NSVLWILSPICIRGPSRRTVA	-2.105	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22871	RFRATMRSILIMLIGGLT			18	SLAY-screened peptide P1221			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTTCCGCGCTACTATGAGGTCTATACTGATAATGCTTATAGGAGGTCTTACATGAATTAAC	RFRATMRSILIMLIGGLT*IN	-2.104	0.003267	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22872	CFQFRSHYHLSRTNMYHYYN			20	SLAY-screened peptide P1222			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTTTCAGTTCCGTTCCCACTACCACCTTTCGCGGACCAATATGTACCACTACTATAATTAA	CFQFRSHYHLSRTNMYHYYN*	-2.103	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22873	CRQLLLEHYNTILDSIVFDH			20	SLAY-screened peptide P1223			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCGTCAGCTCCTGTTGGAGCACTACAATACTATCCTCGATAGTATCGTCTTTGACCATTAA	CRQLLLEHYNTILDSIVFDH*	-2.103	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22874	PIYTNCLYSTDSLTNNSRWF			20	SLAY-screened peptide P1224			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATTTATACGAATTGTTTGTACAGTACTGACAGCCTCACCAATAATTCTCGTTGGTTTTAA	PIYTNCLYSTDSLTNNSRWF*	-2.103	0.000324	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22875	PLDPSYGLIGAPRTVNEWAH			20	SLAY-screened peptide P1225			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTCGATCCTTCTTATGGTCTCATCGGGGCCCCGCGGACCGTCAATGAGTGGGCCCACTAA	PLDPSYGLIGAPRTVNEWAH*	-2.102	0.005148	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22876	VLTTHYDSATNY			12	SLAY-screened peptide P1226			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTTGACCACTCACTATGATTCTGCTACCAATTACTAGCGCCCCTACAATGACTGCTATTAA	VLTTHYDSATNY*RPYNDCY*	-2.102	8.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22877	RATYQGSSYQLIMY			14	SLAY-screened peptide P1227			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGCTACCTATCAGGGCAGTTCTTATCAGCTTATCATGTACTAGACCGGGAGTTACAAGTAA	RATYQGSSYQLIMY*TGSYK*	-2.102	3.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22878	RTTPSLAYRSHRVHSYMGDN			20	SLAY-screened peptide P1228			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACTACGCCGTCCCTCGCCTATCGTTCGCACCGTGTTCACAGCTATATGGGTGATAACTAA	RTTPSLAYRSHRVHSYMGDN*	-2.1	0.003349	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22879	PGMPSPSFAGQRNMPCSRSG			20	SLAY-screened peptide P1229			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGCATGCCGAGTCCTAGTTTCGCCGGCCAGAGGAATATGCCGTGTTCGCGGTCTGGGTAA	PGMPSPSFAGQRNMPCSRSG*	-2.1	0.002396	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22880	PNNFSANTIKHFSRFDGYCS			20	SLAY-screened peptide P1230			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAATAATTTCTCTGCCAATACTATCAAGCATTTTTCTCGTTTCGACGGTTATTGTAGCTAA	PNNFSANTIKHFSRFDGYCS*	-2.099	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22881	VRYNMSTNPSHSSHRVKCII			20	SLAY-screened peptide P1231			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCGTTATAATATGTCTACGAACCCTTCTCATAGTTCCCATCGTGTTAAGTGTATCATCTAA	VRYNMSTNPSHSSHRVKCII*	-2.099	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22882	YYDFGTRVTPYIVHTCYYTG			20	SLAY-screened peptide P1232			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTATGATTTCGGCACTCGCGTCACGCCTTATATTGTTCACACTTGTTATTACACTGGTTAA	YYDFGTRVTPYIVHTCYYTG*	-2.098	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22883	RELPWHSPRILFFLHLLSKA			20	SLAY-screened peptide P1233			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGAGCTTCCCTGGCACTCCCCCCGCATCTTGTTTTTTCTTCATTTGCTGTCCAAGGCCTAA	RELPWHSPRILFFLHLLSKA*	-2.098	0.001361	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22884	TNVSRDYRAHIVSWRLCAQP			20	SLAY-screened peptide P1234			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAACGTGTCCAGGGACTATCGGGCTCACATTGTGTCGTGGCGTCTGTGCGCTCAGCCGTAA	TNVSRDYRAHIVSWRLCAQP*	-2.097	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22885	ISFLCFGLRFTAFLCLRRLRN			21	SLAY-screened peptide P1235			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTCTTTTCTGTGTTTTGGCCTACGTTTTACCGCCTTCTTATGTCTCCGTCGACTACGTAAC	ISFLCFGLRFTAFLCLRRLRN	-2.097	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22886	KIFNRNTNLYCYNYHHVVPP			20	SLAY-screened peptide P1236			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGATCTTCAACAGGAATACCAACCTTTATTGCTATAACTACCACCACGTTGTTCCTCCGTAA	KIFNRNTNLYCYNYHHVVPP*	-2.096	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22887	HHPFDLVWLLLMGNWHNMEN			20	SLAY-screened peptide P1237			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCACCCGTTTGATCTGGTTTGGCTGCTTCTTATGGGCAACTGGCATAATATGGAGAATTAA	HHPFDLVWLLLMGNWHNMEN*	-2.096	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22888	YIYRLWRNGIIIITPMAGIT			20	SLAY-screened peptide P1238			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATATCTATCGCCTTTGGCGTAATGGTATTATCATTATTACTCCGATGGCTGGCATCACGTAA	YIYRLWRNGIIIITPMAGIT*	-2.095	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22889	TTQSVARGTTSQSYFNNATH			20	SLAY-screened peptide P1239			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGACGCAGTCCGTCGCCCGTGGGACTACCTCTCAGTCTTACTTTAATAACGCTACTCACTAA	TTQSVARGTTSQSYFNNATH*	-2.095	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22890	SPALDGIMFQYQAEPGSRAA			20	SLAY-screened peptide P1240			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCCTGCGCTGGATGGTATTATGTTCCAGTATCAGGCCGAGCCTGGCTCGCGCGCGGCCTAA	SPALDGIMFQYQAEPGSRAA*	-2.095	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22891	PPIRPYPTFHTKACTTRSLRN			21	SLAY-screened peptide P1241			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTATCCGTCCGTATCCTACTTTTCACACTAAGGCGTGTACTACGAGATCGTTGCGTAAC	PPIRPYPTFHTKACTTRSLRN	-2.094	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22892	PRSTCTPLQTKANLLTPCQL			20	SLAY-screened peptide P1242			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGTTCTACGTGTACGCCCCTCCAGACTAAGGCTAACCTCCTCACTCCGTGTCAGCTCTAA	PRSTCTPLQTKANLLTPCQL*	-2.094	0.00022	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22893	FAVLPHPNLNFVSSSVYEAV			20	SLAY-screened peptide P1243			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGCGGTTCTGCCTCATCCTAACCTTAATTTCGTTTCCTCGTCCGTCTACGAGGCCGTCTAA	FAVLPHPNLNFVSSSVYEAV*	-2.094	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22894	RTACVHIRYELVTRRRGRRA			20	SLAY-screened peptide P1244			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACTGCTTGTGTCCACATCCGTTACGAGTTGGTCACCAGGCGGCGTGGGCGCCGCGCGTAA	RTACVHIRYELVTRRRGRRA*	-2.094	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22895	RLSCHFVAHIFRPNIFNEHT			20	SLAY-screened peptide P1245			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCTCAGTTGTCATTTCGTTGCGCATATTTTTCGGCCCAATATTTTCAATGAGCACACCTAA	RLSCHFVAHIFRPNIFNEHT*	-2.093	0.00183	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22896	CAYHWNNAASDTYNVHLYTS			20	SLAY-screened peptide P1246			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGCCTACCACTGGAATAACGCTGCGTCGGACACTTATAACGTGCATCTTTATACGTCCTAA	CAYHWNNAASDTYNVHLYTS*	-2.093	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22897	HSGPCRYGIH			10	SLAY-screened peptide P1247			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTCTGGTCCTTGTAGGTACGGCATTCATTAGCCCTCTGTGCATAATAGTGGCTACCGTTAA	HSGPCRYGIH*PSVHNSGYR*	-2.093	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22898	ILSRNSPDAPHTQNLAASPY			20	SLAY-screened peptide P1248			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCTCTCCAGGAACAGTCCCGATGCGCCGCACACCCAGAATCTCGCGGCTTCTCCGTATTAA	ILSRNSPDAPHTQNLAASPY*	-2.092	6.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22899	FYMCINSLRTATRAP			15	SLAY-screened peptide P1249			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTACATGTGTATTAATTCTCTGCGTACTGCTACTCGGGCCCCGTAGCACCTGGATTCGTAA	FYMCINSLRTATRAP*HLDS*	-2.091	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22900	GSSPVATISNYHIHYRPGAL			20	SLAY-screened peptide P1250			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTCCTCCCCGGTGGCCACCATCTCCAACTACCACATTCATTACAGGCCGGGCGCGCTCTAA	GSSPVATISNYHIHYRPGAL*	-2.091	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22901	DPRNYISGEHIGSSNN			16	SLAY-screened peptide P1251			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCCGCGTAACTACATCTCTGGCGAGCATATCGGGTCGAGTAATAATTAGAGTATTTCCTAA	DPRNYISGEHIGSSNN*SIS*	-2.091	0.000176	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22902	PKRRRTFRPTLVTIRIRRSLN			21	SLAY-screened peptide P1252			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAAACGACGCCGCACATTTAGACCCACCCTCGTAACCATCCGCATACGTCGATCTCTTAAC	PKRRRTFRPTLVTIRIRRSLN	-2.09	0.001394	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22903	NYANYVSAVQRGSSHTHINS			20	SLAY-screened peptide P1253			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTACGCTAATTATGTTAGCGCGGTTCAGCGCGGTAGCTCGCATACGCATATTAATTCCTAA	NYANYVSAVQRGSSHTHINS*	-2.09	3.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22904	HTRLPTDGFCTLFKVTLLICN			21	SLAY-screened peptide P1254			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACTCGTCTTCCTACTGATGGCTTTTGCACCCTCTTCAAAGTAACACTGTTGATTTGTAAC	HTRLPTDGFCTLFKVTLLICN	-2.089	0.001995	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22905	PAFSPCFRSPRENRAHLLPF			20	SLAY-screened peptide P1255			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCTTTCTCCCCTTGCTTCCGCAGTCCGAGGGAGAATAGGGCTCATCTCCTGCCGTTCTAA	PAFSPCFRSPRENRAHLLPF*	-2.088	2.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22906	CRTMQAHNWL			10	SLAY-screened peptide P1256			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGCACGATGCAGGCTCATAATTGGCTTTAGGACGTCTCTTCTTACCACAACTCCTTTTAA	CRTMQAHNWL*DVSSYHNSF*	-2.087	0.000785	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22907	CQVCNAL			7	SLAY-screened peptide P1257			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCAGGTGTGTAATGCGCTTTAGGCCCCTTTTGAGGTGGTCCTTAACCTTCTGTAGCATTAA	CQVCNAL*APFEVVLNLL*H*	-2.087	0.000449	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22908	TCPKNPHSRTGVTTSKTKGL			20	SLAY-screened peptide P1258			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTGCCCGAAGAACCCTCATTCCAGGACCGGTGTTACGACGTCGAAGACTAAGGGGTTGTAA	TCPKNPHSRTGVTTSKTKGL*	-2.087	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22909	PADLHDPFPIALRIRRPRRQ			20	SLAY-screened peptide P1259			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCCGATTTGCACGACCCGTTCCCGATTGCTCTCCGGATCCGCCGTCCCCGTCGCCAGTAA	PADLHDPFPIALRIRRPRRQ*	-2.086	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22910	TFYNEAYHQIIGCNIGNTVL			20	SLAY-screened peptide P1260			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTTTTATAACGAGGCTTACCACCAGATTATTGGTTGTAATATCGGCAATACCGTGCTTTAA	TFYNEAYHQIIGCNIGNTVL*	-2.085	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22911	CDVPPPFNTPLSYGWYNMGH			20	SLAY-screened peptide P1261			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGACGTGCCTCCCCCCTTTAACACTCCGCTCAGTTATGGTTGGTATAATATGGGCCATTAA	CDVPPPFNTPLSYGWYNMGH*	-2.084	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22912	HLSHTPRSFHNVHTAHRRVC			20	SLAY-screened peptide P1262			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCTTAGTCACACGCCGCGTTCTTTTCATAATGTCCACACGGCTCATCGTCGTGTTTGCTAA	HLSHTPRSFHNVHTAHRRVC*	-2.084	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22913	HFCPNLPNKRDAHSSSTSTW			20	SLAY-screened peptide P1263			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTTTTGCCCGAACCTTCCCAATAAGAGGGATGCTCACAGTAGCTCGACTAGCACGTGGTAA	HFCPNLPNKRDAHSSSTSTW*	-2.084	4.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22914	LGRMGMCVLSTTSFKKDTCV			20	SLAY-screened peptide P1264			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGGTCGTATGGGTATGTGCGTGCTCAGCACCACCAGTTTCAAGAAGGACACGTGTGTGTAA	LGRMGMCVLSTTSFKKDTCV*	-2.084	3.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22915	SLLSNAHTSIYQNCRQAFSD			20	SLAY-screened peptide P1265			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCTGCTCTCTAACGCCCATACGTCTATCTATCAGAATTGCCGCCAGGCTTTTTCCGACTAA	SLLSNAHTSIYQNCRQAFSD*	-2.084	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22916	RTPHSYYWSPWDHEVLLAYS			20	SLAY-screened peptide P1266			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACCCCTCATAGTTATTATTGGTCTCCGTGGGACCATGAGGTCCTCCTCGCGTACAGCTAA	RTPHSYYWSPWDHEVLLAYS*	-2.082	0.000177	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22917	EVYRKCNNQIELLFI			15	SLAY-screened peptide P1267			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGTGTACAGGAAGTGCAACAACCAGATTGAGCTGCTCTTCATCTAGATCGCTATGACTTAA	EVYRKCNNQIELLFI*IAMT*	-2.081	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22918	WKATTTPARHLDDSMSDPYL			20	SLAY-screened peptide P1268			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGAAGGCTACTACGACTCCTGCCCGTCACCTCGATGACTCCATGAGCGACCCTTATTTGTAA	WKATTTPARHLDDSMSDPYL*	-2.081	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22919	LSYVGSSSAMSPASLPLFLN			20	SLAY-screened peptide P1269			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCCTATGTGGGAAGTAGTTCTGCAATGTCTCCTGCCTCATTGCCATTGTTTCTTAACTGA	LSYVGSSSAMSPASLPLFLN*	-2.081	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22920	AEIRSASLSTRNFSAQVEST			20	SLAY-screened peptide P1270			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGAGATCCGGTCTGCTTCGCTCTCGACCCGCAACTTCTCGGCCCAGGTGGAGAGTACGTAA	AEIRSASLSTRNFSAQVEST*	-2.08	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22921	DVLIHTSDLMAVRIMLNNLY			20	SLAY-screened peptide P1271			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATGTCCTTATCCACACTTCCGATTTGATGGCCGTCCGTATTATGCTGAATAATCTCTATTAA	DVLIHTSDLMAVRIMLNNLY*	-2.08	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22922	PCYDFLATNISSIDYNYYIL			20	SLAY-screened peptide P1272			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGTTACGATTTTCTTGCGACGAATATCTCGTCGATTGACTATAATTATTACATTTTGTAA	PCYDFLATNISSIDYNYYIL*	-2.08	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22923	RCISSLI			7	SLAY-screened peptide P1273			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGTATTTCTTCTCTTATTTAGTCTATTCTGAAGTTGCATGTTGCGTCTTTTATTATTTAA	RCISSLI*SILKLHVASFII*	-2.078	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22924	IDPLSGDPTSGIFSSADHQI			20	SLAY-screened peptide P1274			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGATCCCCTGTCCGGGGACCCTACTTCCGGCATTTTCTCTAGTGCGGACCATCAGATCTAA	IDPLSGDPTSGIFSSADHQI*	-2.078	0.000335	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22925	TYRAPGCSVYCHTYTLGYNV			20	SLAY-screened peptide P1275			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTACCGGGCGCCTGGTTGCAGTGTTTACTGTCATACGTATACCCTGGGTTATAATGTCTAA	TYRAPGCSVYCHTYTLGYNV*	-2.078	0.003052	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22926	FSHNDCFNRDFPLTRYNQAY			20	SLAY-screened peptide P1276			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAGTCACAATGACTGTTTTAATCGTGATTTTCCGCTCACTAGGTATAACCAGGCCTATTAA	FSHNDCFNRDFPLTRYNQAY*	-2.076	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22927	KLKSLHVLPTSCFPSMDTDL			20	SLAY-screened peptide P1277			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCTTAAGTCCTTGCACGTTCTCCCGACTTCCTGTTTTCCGTCCATGGATACTGACCTTTAA	KLKSLHVLPTSCFPSMDTDL*	-2.076	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22928	LFLKGGRHRFRCTFSYEALK			20	SLAY-screened peptide P1278			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTCCTGAAGGGGGGTCGCCACCGCTTTAGGTGTACCTTCAGTTATGAGGCCTTGAAGTAA	LFLKGGRHRFRCTFSYEALK*	-2.076	0.002542	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22929	LHLLDPAMYNVISIPTNVDT			20	SLAY-screened peptide P1279			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCATTTGCTTGATCCGGCCATGTATAATGTTATCTCTATTCCCACTAACGTTGATACTTAA	LHLLDPAMYNVISIPTNVDT*	-2.075	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22930	VYNKEPNRCCTGPPIINTIS			20	SLAY-screened peptide P1280			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTACAACAAGGAGCCGAACAGGTGCTGCACTGGCCCTCCTATTATTAATACTATCTCTTAA	VYNKEPNRCCTGPPIINTIS*	-2.075	0.002365	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22931	AVYCCGSLLSHITTHLIDYH			20	SLAY-screened peptide P1281			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGTTTATTGCTGCGGCTCTCTTCTGTCCCACATCACTACTCACCTGATCGATTACCATTAA	AVYCCGSLLSHITTHLIDYH*	-2.074	0.001808	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22932	SGSAHSDDLPRAYLYHGSCL			20	SLAY-screened peptide P1282			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGGCTCCGCTCATTCTGATGATCTTCCTAGGGCTTACCTTTACCATGGTTCCTGCCTCTAA	SGSAHSDDLPRAYLYHGSCL*	-2.074	0.001758	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22933	NTDCVRFCYLMIHSPRWFSI			20	SLAY-screened peptide P1283			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATACTGACTGCGTGCGTTTTTGCTACCTTATGATTCATTCTCCGCGTTGGTTTAGCATTTAA	NTDCVRFCYLMIHSPRWFSI*	-2.074	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22934	GVTHVSAKRRSNPARNITT			19	SLAY-screened peptide P1284			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGTCACCCACGTTTCCGCTAAGCGTCGGTCGAATCCTGCTCGTAATATTACGACGTAGTAA	GVTHVSAKRRSNPARNITT**	-2.074	0.002261	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22935	ITLTLAGRPTAHST			14	SLAY-screened peptide P1285			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTACCCTTACCTTGGCTGGCCGCCCTACTGCTCACAGTACCTAGCACTACACCGCTGGCTAA	ITLTLAGRPTAHST*HYTAG*	-2.073	0.000685	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22936	GHGHHNGATKVRCLDLTWQVT			21	SLAY-screened peptide P1286			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCACGGCCACCATAATGGTGCCACTAAGGTTAGGTGCTTGGATCTCACTTGGCAAGTAACT	GHGHHNGATKVRCLDLTWQVT	-2.073	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22937	PCLVYCTDLPAVNLPYISVF			20	SLAY-screened peptide P1287			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGCCTGGTGTATTGCACGGATCTGCCCGCTGTCAACCTTCCCTACATTTCCGTGTTTTAA	PCLVYCTDLPAVNLPYISVF*	-2.073	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22938	SEGNAQPA			8	SLAY-screened peptide P1288			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGAGGGCAACGCCCAGCCTGCTTAGTTTCAGTTCTTCTGCCGTCAGGACCGTTATATTTAA	SEGNAQPA*FQFFCRQDRYI*	-2.073	0.00333	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22939	YAMTGGAWLYTFPEGMVLRY			20	SLAY-screened peptide P1289			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGCGATGACTGGTGGTGCCTGGCTCTATACCTTTCCCGAGGGCATGGTTCTCAGGTACTAA	YAMTGGAWLYTFPEGMVLRY*	-2.072	0.000107	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22940	CTRCHNAGNYPHTYNSSIQDD			21	SLAY-screened peptide P1290			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCACGAGGTGTCACAATGCCGGCAATTATCCTCATACCTATAACTCTTCCATTCAGGACGAT	CTRCHNAGNYPHTYNSSIQDD	-2.072	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22941	YLCIDNNTPYMHSIN			15	SLAY-screened peptide P1291			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCTTTGTATTGACAATAATACGCCTTACATGCACTCCATTAATTAGTTCTATTTTGGTTAA	YLCIDNNTPYMHSIN*FYFG*	-2.072	4.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22942	LSNNRRLHHIAGPRENLVNGN			21	SLAY-screened peptide P1292			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAGTAACAATCGGCGCCTGCACCACATCGCTGGCCCTCGCGAGAACCTTGTTAACGGTAAC	LSNNRRLHHIAGPRENLVNGN	-2.072	0.002805	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22943	TYHYRAPVRSRPLYAGLDLH			20	SLAY-screened peptide P1293			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTACCATTACCGGGCCCCCGTGAGGTCTAGGCCTCTCTACGCGGGGCTCGATCTGCATTAA	TYHYRAPVRSRPLYAGLDLH*	-2.072	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22944	PCSMPTRNIPRRINLTALRL			20	SLAY-screened peptide P1294			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGTTCGATGCCGACTCGCAATATCCCTCGTCGTATTAATTTGACGGCGCTCCGTCTGTAA	PCSMPTRNIPRRINLTALRL*	-2.072	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22945	SALYYYTLCARRNIRSITLY			20	SLAY-screened peptide P1295			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGCCTTGTATTATTACACGCTCTGTGCTCGCCGCAACATCCGTAGTATTACGCTTTATTAA	SALYYYTLCARRNIRSITLY*	-2.071	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22946	HRYLLALSRMYNRMSAVSNA			20	SLAY-screened peptide P1296			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCGGTATCTGCTTGCCCTGTCTCGTATGTATAATCGTATGTCGGCTGTGAGTAATGCTTAA	HRYLLALSRMYNRMSAVSNA*	-2.071	0.000197	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22947	TILAPRYNRVIPTFVAWTPL			20	SLAY-screened peptide P1297			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGATCCTGGCCCCCCGTTACAATCGTGTTATCCCCACTTTTGTTGCTTGGACTCCCCTTTAA	TILAPRYNRVIPTFVAWTPL*	-2.071	8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22948	VVAVAGRNVATVHNAEHNMY			20	SLAY-screened peptide P1298			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGTGGCTGTGGCGGGCCGGAATGTTGCGACCGTCCATAATGCCGAGCACAATATGTATTAA	VVAVAGRNVATVHNAEHNMY*	-2.07	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22949	VGHPPSTLRRWHPFCCSVIV			20	SLAY-screened peptide P1299			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGGGCATCCCCCTAGCACTCTTCGTCGTTGGCACCCCTTTTGCTGCTCTGTCATTGTCTAA	VGHPPSTLRRWHPFCCSVIV*	-2.069	7.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22950	TGRGLSSVRFHDSPSVVNYA			20	SLAY-screened peptide P1300			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGGTAGGGGCCTTAGTTCTGTTCGTTTTCACGATAGTCCGTCCGTCGTTAATTATGCGTAA	TGRGLSSVRFHDSPSVVNYA*	-2.069	0.000325	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22951	YSMPRHSNTCFIS			13	SLAY-screened peptide P1301			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACAGTATGCCCCGGCACAGCAATACTTGTTTCATTTCTTAGTCCGGTTAGACTGACCATTAA	YSMPRHSNTCFIS*SG*TDH*	-2.069	0.004988	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22952	LFHFYCGDLCINSPWWLPGS			20	SLAY-screened peptide P1302			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTTTCATTTTTATTGCGGTGATCTCTGTATTAATAGTCCCTGGTGGCTGCCGGGTAGTTAA	LFHFYCGDLCINSPWWLPGS*	-2.069	0.004447	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22953	HLICVTIRSRPRRRAGTVSN			20	SLAY-screened peptide P1303			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCTGATCTGCGTCACTATCCGCTCACGGCCTCGCCGACGTGCAGGCACAGTTAGTAACTGA	HLICVTIRSRPRRRAGTVSN*	-2.067	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22954	AIVSHPTPQADPTNPTREYN			20	SLAY-screened peptide P1304			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGATCGTCAGCCACCCGACGCCTCAGGCTGACCCTACGAACCCTACCAGGGAGTATAATTAA	AIVSHPTPQADPTNPTREYN*	-2.067	0.000336	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22955	HYLTFSNIPIAFLDTWE			17	SLAY-screened peptide P1305			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTATTTGACTTTCAGTAATATCCCGATCGCTTTTCTCGATACCTGGGAGTAGAAGCAGTAA	HYLTFSNIPIAFLDTWE*KQ*	-2.067	0.008719	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22956	SVYLDYTRRTRLTFLTTGNQ			20	SLAY-screened peptide P1306			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGTGTACCTCGATTATACCAGGCGTACGAGGCTGACTTTCCTTACTACTGGTAATCAGTAA	SVYLDYTRRTRLTFLTTGNQ*	-2.067	0.000844	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22957	PFKDYRCHYQALHADNPGSA			20	SLAY-screened peptide P1307			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTTAAGGATTACAGGTGCCATTACCAGGCTCTTCACGCTGATAACCCTGGCAGTGCCTAA	PFKDYRCHYQALHADNPGSA*	-2.066	0.000289	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22958	AQLRAKYIPTSDGFQCSKYA			20	SLAY-screened peptide P1308			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCAGCTGCGGGCTAAGTACATTCCTACCAGTGATGGCTTCCAGTGCTCGAAGTACGCTTAA	AQLRAKYIPTSDGFQCSKYA*	-2.065	9.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22959	NKTRPLSNHNLVAVLRLMS			19	SLAY-screened peptide P1309			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATAAGACTAGGCCGCTGTCGAACCATAATCTTGTGGCTGTGCTTAGGCTTATGTCTTAGTAA	NKTRPLSNHNLVAVLRLMS**	-2.065	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22960	DIQRKSYRSCISIYYLTRTI			20	SLAY-screened peptide P1310			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACATTCAGCGCAAGTCTTACCGCAGCTGCATTTCGATTTACTACCTTACTAGGACTATTTAA	DIQRKSYRSCISIYYLTRTI*	-2.064	0.000166	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22961	YTVSVSTCATAFTVDTSKQD			20	SLAY-screened peptide P1311			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACCGTCTCTGTCTCCACCTGTGCCACCGCTTTTACTGTCGACACGTCGAAGCAGGATTAA	YTVSVSTCATAFTVDTSKQD*	-2.063	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22962	SPCTLVGIAHSLHDDNPVHH			20	SLAY-screened peptide P1312			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCCTTGCACCCTGGTTGGCATTGCTCATTCTTTGCATGACGACAACCCCGTCCACCATTAA	SPCTLVGIAHSLHDDNPVHH*	-2.063	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22963	RAPDTLASEPYVPTTGYSHS			20	SLAY-screened peptide P1313			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGCTCCTGATACGCTCGCCTCGGAGCCCTATGTCCCTACGACCGGCTACTCGCATTCCTAA	RAPDTLASEPYVPTTGYSHS*	-2.062	0.005441	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22964	HYRNLLTVDNHGTYSIKNFC			20	SLAY-screened peptide P1314			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTACCGTAACCTTCTTACTGTTGATAATCATGGCACTTACTCGATTAAGAACTTCTGCTAA	HYRNLLTVDNHGTYSIKNFC*	-2.061	0.000534	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22965	HADNLTSQPLFHNTNDRHYS			20	SLAY-screened peptide P1315			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGCTGATAATCTCACCTCTCAGCCTCTCTTTCATAATACTAATGATCGTCACTATTCCTAA	HADNLTSQPLFHNTNDRHYS*	-2.061	2.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22966	IHLFFICIISTALTRRRPRSN			21	SLAY-screened peptide P1316			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCATCTGTTCTTCATATGCATCATTAGCACTGCTCTAACTAGACGCAGGCCTCGTAGTAAC	IHLFFICIISTALTRRRPRSN	-2.061	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22967	YSLIGIILAAYFRCHHSLSS			20	SLAY-screened peptide P1317			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTCTCTTATTGGGATTATTCTCGCCGCCTACTTTAGGTGCCATCACAGTTTGAGTAGCTAA	YSLIGIILAAYFRCHHSLSS*	-2.061	0.003834	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22968	HMPHTTWHVH			10	SLAY-screened peptide P1318			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACATGCCGCATACCACTTGGCATGTGCACTAGAGGGCGACGACGAGTCATCCTTCTCTCTAA	HMPHTTWHVH*RATTSHPSL*	-2.06	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22969	RLPWLWYIVYLKMPLLARIAN			21	SLAY-screened peptide P1319			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCTGCCCTGGCTGTGGTACATTGTCTATCTTAAGATGCCCCTCCTAGCAAGAATTGCTAAC	RLPWLWYIVYLKMPLLARIAN	-2.059	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22970	IPSLSFTIILS			11	SLAY-screened peptide P1320			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCCTAGCCTAAGTTTCACTATAATTTTAAGTTGATTTGGCGTAAGACCGCTCACTTTTAAC	IPSLSFTIILS*FGVRPLTFN	-2.059	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22971	NDQHYTALCHYCCVDPGCYT			20	SLAY-screened peptide P1321			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGATCAGCACTACACGGCGCTCTGCCATTATTGTTGTGTTGACCCGGGTTGTTACACTTAA	NDQHYTALCHYCCVDPGCYT*	-2.059	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22972	PPYTAAPPFVFRVNSALNSH			20	SLAY-screened peptide P1322			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCTACACGGCCGCTCCTCCTTTTGTTTTCCGCGTTAACTCCGCTCTCAATAGCCACTAA	PPYTAAPPFVFRVNSALNSH*	-2.058	0.000578	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22973	RRCTHDMYLLTSYEPLLRNT			20	SLAY-screened peptide P1323			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAGGTGCACCCACGACATGTATCTCCTGACCAGCTACGAGCCCCTGCTGCGCAACACTTAA	RRCTHDMYLLTSYEPLLRNT*	-2.058	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22974	CRPSNKYAPCRPSLRHILYF			20	SLAY-screened peptide P1324			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGCCCTTCTAACAAGTACGCTCCTTGCCGCCCTTCGCTTCGCCACATTTTGTACTTTTAA	CRPSNKYAPCRPSLRHILYF*	-2.058	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22975	DSSAPYLYFLIIFTARSRWS			20	SLAY-screened peptide P1325			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATAGTTCTGCCCCGTACCTCTATTTTCTCATCATCTTCACCGCTCGCTCCCGTTGGTCGTAA	DSSAPYLYFLIIFTARSRWS*	-2.057	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22976	PVHTRTALPGPCTIRPQVRP			20	SLAY-screened peptide P1326			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGTCCACACTCGTACGGCTTTGCCCGGCCCTTGCACTATCCGCCCGCAGGTCAGGCCGTAA	PVHTRTALPGPCTIRPQVRP*	-2.057	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22977	ATYLWTHNYHLIVYPTTRPR			20	SLAY-screened peptide P1327			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGACCTACCTTTGGACGCACAATTACCACCTTATTGTGTATCCTACCACGCGCCCTCGCTAA	ATYLWTHNYHLIVYPTTRPR*	-2.055	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22978	DQLPLDTWLPLLGCHPLIRI			20	SLAY-screened peptide P1328			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCAGCTCCCCCTGGACACTTGGTTGCCTCTTCTCGGGTGTCATCCCCTTATTCGCATTTAA	DQLPLDTWLPLLGCHPLIRI*	-2.055	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22979	PYHKMSLNAVMSVSELYLTP			20	SLAY-screened peptide P1329			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTATCATAAGATGTCTCTCAATGCTGTTATGAGTGTGAGTGAGTTGTACTTGACTCCCTAA	PYHKMSLNAVMSVSELYLTP*	-2.054	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22980	CHVPYLHKGTYVPAFTLDEP			20	SLAY-screened peptide P1330			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCACGTTCCGTACCTCCATAAGGGTACCTATGTCCCCGCGTTCACGCTTGATGAGCCCTAA	CHVPYLHKGTYVPAFTLDEP*	-2.052	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22981	LTPTPTATRHRTFASNNVYK			20	SLAY-screened peptide P1331			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACTCCTACTCCTACCGCCACGCGGCACCGCACTTTTGCTTCGAACAATGTCTACAAGTAA	LTPTPTATRHRTFASNNVYK*	-2.052	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22982	TLNNT			5	SLAY-screened peptide P1332			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTGAATAACACGTAGCCTATGCAGGAGCTCACTGGCGGCGTCATCAACAACTGCATTTAA	TLNNT*PMQELTGGVINNCI*	-2.052	0.000205	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22983	NSWKGTRG			8	SLAY-screened peptide P1333			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTCCTGGAAGGGTACCCGAGGCTGATCTTTATTGAGTAGACTTATCCCCCTACCCTTTAAC	NSWKGTRG*SLLSRLIPLPFN	-2.051	0.006186	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22984	PMGVAPIYGPPLDAANFSLH			20	SLAY-screened peptide P1334			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATGGGCGTGGCTCCGATTTATGGTCCTCCTCTCGACGCTGCTAACTTCTCTCTGCATTAA	PMGVAPIYGPPLDAANFSLH*	-2.051	4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22985	SRSQYYFPVDFMALRLLLSLN			21	SLAY-screened peptide P1335			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCGCAGCCAGTACTACTTCCCTGTCGACTTTATGGCGCTCCGTTTGCTACTTTCGCTTAAC	SRSQYYFPVDFMALRLLLSLN	-2.051	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22986	APIYNHNCVMLCINMAPRHP			20	SLAY-screened peptide P1336			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCCGATTTATAACCACAATTGTGTTATGCTTTGCATCAATATGGCTCCGCGTCATCCCTAA	APIYNHNCVMLCINMAPRHP*	-2.051	2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22987	LPHNIWHRTGLLTHNWTARS			20	SLAY-screened peptide P1337			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCTCATAATATCTGGCATCGGACCGGCCTGTTGACTCACAATTGGACCGCTCGCTCCTAA	LPHNIWHRTGLLTHNWTARS*	-2.051	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22988	PHVRDKPSRARIFTSTWHVI			20	SLAY-screened peptide P1338			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCACGTCCGGGACAAGCCCTCCCGGGCTAGGATCTTCACCTCCACTTGGCATGTTATTTAA	PHVRDKPSRARIFTSTWHVI*	-2.051	0.000673	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22989	LKLATCLYNMHCQ			13	SLAY-screened peptide P1339			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAAGCTCGCGACGTGTCTTTACAATATGCATTGCCAGTAGGGTAATGACATTCATGTTTAA	LKLATCLYNMHCQ*GNDIHV*	-2.05	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22990	NVREPVHNHDNWIRGPPLFLT			21	SLAY-screened peptide P1340			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGTCCGCGAGCCCGTGCACAACCATGACAACTGGATCCGGGGCCCGCCCTTGTTCCTAACT	NVREPVHNHDNWIRGPPLFLT	-2.05	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22991	VHNSVLSPLNYDLFTSRKYA			20	SLAY-screened peptide P1341			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCACAATTCCGTGCTGAGCCCCCTCAATTATGACCTTTTTACTTCCAGGAAGTACGCTTAA	VHNSVLSPLNYDLFTSRKYA*	-2.049	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22992	NAYWTNMILCIIAFWLHTNL			20	SLAY-screened peptide P1342			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGCCTATTGGACTAACATGATCTTGTGTATCATCGCCTTTTGGCTTCACACGAACCTTTAA	NAYWTNMILCIIAFWLHTNL*	-2.049	0.000529	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22993	IR			2	SLAY-screened peptide P1343			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCGTTAGGACGACGCTGACTACCCCGCGGTGCTTACTTCGACCACTTATACGCGTCCGTAA	IR*DDADYPAVLTSTTYTRP*	-2.048	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22994	WAYNNPDD			8	SLAY-screened peptide P1344			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGGCGTACAACAACCCGGACGACTAGAGCCCGTTTCATTTTAACCTTTTGCCGTGCCCTTAA	WAYNNPDD*SPFHFNLLPCP*	-2.048	0.00115	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22995	TFFFLILFIHVNRNTVHPID			20	SLAY-screened peptide P1345			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTTTTTCTTTTTGATCTTGTTTATCCATGTGAATCGTAATACTGTTCATCCCATCGACTAA	TFFFLILFIHVNRNTVHPID*	-2.047	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22996	LYHARLYRLYLVASVDSVHS			20	SLAY-screened peptide P1346			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTACCACGCCCGCCTCTACCGCCTTTACCTCGTGGCGTCCGTGGACTCGGTTCACTCGTAA	LYHARLYRLYLVASVDSVHS*	-2.047	0.002145	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22997	RPSAFCLRNSNSIEFDRPTM			20	SLAY-screened peptide P1347			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCCAGCGCCTTTTGTCTCCGTAACTCTAATTCGATCGAGTTCGATCGTCCTACTATGTAA	RPSAFCLRNSNSIEFDRPTM*	-2.047	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22998	PDIYHRINR			9	SLAY-screened peptide P1348			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGATATCTATCACAGGATCAACCGCTAGCTGTGGACTCTGGGTAGTATTCGGAGGACTTAA	PDIYHRINR*LWTLGSIRRT*	-2.046	0.00019	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP22999	RASSHNSFNH			10	SLAY-screened peptide P1349			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGCTTCTAGCCACAATTCCTTCAACCATTAGCTCAGTTTTCATAAGTGTTTCCATAAGTAA	RASSHNSFNH*LSFHKCFHK*	-2.046	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23000	GKLYQYVPDIICLITRLITW			20	SLAY-screened peptide P1350			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTAAGCTTTATCAGTATGTTCCCGATATTATTTGCCTTATTACCCGTCTGATTACGTGGTAA	GKLYQYVPDIICLITRLITW*	-2.046	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23001	GMLDYRPSTYTNLGTTHTGGL			21	SLAY-screened peptide P1351			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCATGTTGGACTACCGCCCTTCGACCTACACGAATCTTGGTACGACGCATACTGGTGGGCTG	GMLDYRPSTYTNLGTTHTGGL	-2.045	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23002	APGRPRPAEPSNNAYSAPM			19	SLAY-screened peptide P1352			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCCTGGTCGTCCCCGCCCGGCTGAGCCCAGCAACAATGCCTACAGTGCCCCTATGTAGTAA	APGRPRPAEPSNNAYSAPM**	-2.045	0.007005	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23003	LVVNFRYYKEESNLVPPDFD			20	SLAY-screened peptide P1353			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGTCGTTAACTTTAGGTATTATAAGGAGGAGTCCAATCTCGTCCCGCCGGACTTCGATTAA	LVVNFRYYKEESNLVPPDFD*	-2.045	0.000913	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23004	LPLNPVDSDAVFTSLREPLY			20	SLAY-screened peptide P1354			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCCGCTCAATCCTGTTGATAGTGATGCTGTTTTTACCTCCCTCCGCGAGCCTCTTTATTAA	LPLNPVDSDAVFTSLREPLY*	-2.044	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23005	STFVVS			6	SLAY-screened peptide P1355			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCACTTTCGTGGTGTCCTAACGCGCGGCCCTATCCTCCTTCTGTATGCTGCCTCGGGGTAAC	STFVVS*RAALSSFCMLPRGN	-2.043	0.005663	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23006	DSMPLFLSCSPSYPSKTPNR			20	SLAY-screened peptide P1356			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTCCATGCCCTTGTTTCTTTCGTGCAGTCCCTCTTACCCGTCTAAGACTCCCAACCGTTAA	DSMPLFLSCSPSYPSKTPNR*	-2.042	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23007	HTKNSVHIKSCLPVSYRHRP			20	SLAY-screened peptide P1357			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACGAAGAATAGCGTCCACATTAAGTCGTGTTTGCCCGTGTCTTACCGGCACAGGCCCTAA	HTKNSVHIKSCLPVSYRHRP*	-2.041	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23008	LHDVIGFNRIFILPPILNYN			20	SLAY-screened peptide P1358			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCATGACGTTATCGGGTTCAATCGTATTTTTATTCTTCCCCCGATCCTCAATTATAACTAA	LHDVIGFNRIFILPPILNYN*	-2.041	0.001454	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23009	HAPMQRLHCRASPFLFVLVV			20	SLAY-screened peptide P1359			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCTCCCATGCAGCGTCTCCATTGTCGCGCGAGTCCCTTTCTTTTTGTGCTGGTTGTGTAA	HAPMQRLHCRASPFLFVLVV*	-2.041	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23010	SPDSLKPTRHFFLSTFKTVR			20	SLAY-screened peptide P1360			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCCCGATAGTCTTAAGCCTACGCGCCATTTCTTTCTTAGCACTTTTAAGACCGTTCGTTAA	SPDSLKPTRHFFLSTFKTVR*	-2.04	0.004689	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23011	PPAGMNASVQYTRYRV			16	SLAY-screened peptide P1361			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCGCCGGCATGAATGCTTCTGTTCAGTACACTCGGTATCGCGTTTAGAGTAAGGGGTAA	PPAGMNASVQYTRYRV*SKG*	-2.04	0.004712	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23012	LRILYTIRINIDLSDYNVSA			20	SLAY-screened peptide P1362			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAGGATCCTTTACACTATCCGTATCAATATTGATCTCTCTGACTATAACGTGTCGGCGTAA	LRILYTIRINIDLSDYNVSA*	-2.04	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23013	PTRSLGRLLRFTMCRRRRSIN			21	SLAY-screened peptide P1363			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACTCGTTCTTTGGGGCGTTTACTCCGATTCACCATGTGTCGTAGACGCCGAAGCATTAAC	PTRSLGRLLRFTMCRRRRSIN	-2.039	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23014	FPNYKGRTRSASPYHPYSIY			20	SLAY-screened peptide P1364			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCCCAATTACAAGGGGCGCACGAGGTCCGCCAGTCCTTACCACCCGTATTCTATCTACTAA	FPNYKGRTRSASPYHPYSIY*	-2.039	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23015	SHTLALHTARLYIDCVHCNR			20	SLAY-screened peptide P1365			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCACACCCTGGCGCTTCACACCGCCCGGCTTTACATTGATTGTGTTCATTGTAATCGGTAA	SHTLALHTARLYIDCVHCNR*	-2.039	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23016	PFLLSQSSMDQCFRESTDRT			20	SLAY-screened peptide P1366			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTCCTGCTTAGTCAGTCGTCGATGGACCAGTGTTTCAGGGAGAGCACCGACCGTACCTAA	PFLLSQSSMDQCFRESTDRT*	-2.039	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23017	GTEAFRAYITRYASCILTYL			20	SLAY-screened peptide P1367			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGACCGAGGCCTTCCGTGCGTATATTACTCGTTACGCTAGTTGCATCCTGACCTATCTGTAA	GTEAFRAYITRYASCILTYL*	-2.039	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23018	FISTRVTNVQAIRGHPMFRA			20	SLAY-screened peptide P1368			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTATCTCTACCCGCGTTACTAATGTTCAGGCTATCCGCGGCCACCCTATGTTTAGGGCGTAA	FISTRVTNVQAIRGHPMFRA*	-2.039	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23019	SCLALLLCYLKKLTLRAVHR			20	SLAY-screened peptide P1369			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTGCCTCGCGCTTCTCTTGTGCTACCTTAAGAAGTTGACTCTCCGGGCCGTGCACAGGTAA	SCLALLLCYLKKLTLRAVHR*	-2.038	0.000601	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23020	RSARHNCLSWDLPNSTSLLRN			21	SLAY-screened peptide P1370			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTCTGCCCGTCACAACTGCCTCAGCTGGGACCTGCCCAATTCCACTTCATTACTTCGTAAC	RSARHNCLSWDLPNSTSLLRN	-2.038	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23021	VHHMPNVTRHSITGFHLNDTN			21	SLAY-screened peptide P1371			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCACCACATGCCTAACGTTACGAGGCATAGCATTACTGGGTTTCACCTTAACGACACGAAC	VHHMPNVTRHSITGFHLNDTN	-2.038	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23022	SGLALSYMTVCYVNSHYYSY			20	SLAY-screened peptide P1372			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGGTTTGGCCCTCAGCTATATGACGGTGTGTTATGTCAATAGTCACTACTACTCTTACTAA	SGLALSYMTVCYVNSHYYSY*	-2.038	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23023	HISNGYRIWFLFCYHFYCRT			20	SLAY-screened peptide P1373			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATATCTCCAACGGGTATCGCATTTGGTTCCTGTTTTGTTATCACTTTTACTGTCGCACCTAA	HISNGYRIWFLFCYHFYCRT*	-2.038	0.000356	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23024	CDFRMPTYKYNYDII			15	SLAY-screened peptide P1374			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGATTTCCGCATGCCTACTTACAAGTACAATTATGACATTATTTAGTTCTCGACGGCGTAA	CDFRMPTYKYNYDII*FSTA*	-2.037	0.000528	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23025	DPTNNTYSTRIQGSGDSPVY			20	SLAY-screened peptide P1375			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCCTACTAACAACACCTATTCGACGCGTATTCAGGGTAGTGGCGACAGTCCGGTTTATTAA	DPTNNTYSTRIQGSGDSPVY*	-2.036	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23026	IPSRRQATMAPSYTSF			16	SLAY-screened peptide P1376			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCCCTCGCGCCGTCAGGCCACTATGGCCCCGAGCTATACCTCGTTTTAGACCCCGCTCTAA	IPSRRQATMAPSYTSF*TPL*	-2.036	0.00012	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23027	LYRASTQRTSGDCGFLFLCL			20	SLAY-screened peptide P1377			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTACCGCGCCTCTACGCAGCGTACCTCTGGCGATTGTGGCTTCTTGTTTCTGTGCCTGTAA	LYRASTQRTSGDCGFLFLCL*	-2.036	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23028	FSANGDATM			9	SLAY-screened peptide P1378			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTCCGCTAATGGGGATGCTACCATGTAGACTTTCGTCTATAATAGTCTTACTAATGAGTAA	FSANGDATM*TFVYNSLTNE*	-2.036	5.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23029	PSNLLYHRVHLSPDMHCWGT			20	SLAY-screened peptide P1379			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGCAATTTGCTCTACCATCGTGTGCACTTGAGTCCTGATATGCACTGCTGGGGCACGTAA	PSNLLYHRVHLSPDMHCWGT*	-2.036	0.00205	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23030	HGIDTHIIYPSAFNYTHTEH			20	SLAY-screened peptide P1380			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGGCATTGATACTCACATTATCTATCCTAGTGCCTTCAACTACACGCATACTGAGCATTAA	HGIDTHIIYPSAFNYTHTEH*	-2.035	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23031	LSPGLATILRFLSLALALRLL			21	SLAY-screened peptide P1381			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAGTCCCGGGCTTGCTACAATCCTAAGATTCCTTTCCCTCGCTCTAGCCTTACGGCTCCTT	LSPGLATILRFLSLALALRLL	-2.035	0.000762	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23032	TLVSSSNF			8	SLAY-screened peptide P1382			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTTGTGTCGAGTTCTAACTTCTAGAGCGTCTGTGAGTACGCGATCAACGATTCCTCCTAA	TLVSSSNF*SVCEYAINDSS*	-2.035	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23033	YPRSAVSTFCFNQGRYSFDV			20	SLAY-screened peptide P1383			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCCCCGCTCTGCGGTTTCCACCTTCTGCTTTAATCAGGGTAGGTACTCTTTTGACGTGTAA	YPRSAVSTFCFNQGRYSFDV*	-2.034	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23034	QATLANTFDLERYDRNKLPD			20	SLAY-screened peptide P1384			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGCGACGCTGGCTAATACGTTTGACCTCGAGCGGTACGACCGTAACAAGCTGCCGGATTAA	QATLANTFDLERYDRNKLPD*	-2.034	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23035	NTSPPDKRASSHLRRQTACY			20	SLAY-screened peptide P1385			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACACCAGCCCTCCCGACAAGCGCGCGAGTTCTCATCTCCGCCGTCAGACTGCTTGTTACTAA	NTSPPDKRASSHLRRQTACY*	-2.033	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23036	ISSNCVHH			8	SLAY-screened peptide P1386			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTTCTAGTAACTGCGTTCATCATTAGGCCAATAATGGGATTATCTTCACGAGGACTTATTAA	ISSNCVHH*ANNGIIFTRTY*	-2.032	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23037	VRTNLPIDSLPSARSYENST			20	SLAY-screened peptide P1387			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCGGACCAACTTGCCTATTGATTCTCTCCCTTCTGCCCGTTCTTACGAGAATTCGACGTAA	VRTNLPIDSLPSARSYENST*	-2.031	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23038	LPLRKSHHAA			10	SLAY-screened peptide P1388			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCTCTGCGCAAGAGCCATCATGCGGCTTAGTGTAACCTGCACACTTCGAACAATATTTAA	LPLRKSHHAA*CNLHTSNNI*	-2.029	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23039	RFTHICGNIGYHNFNIITSS			20	SLAY-screened peptide P1389			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTTTACGCATATTTGTGGTAACATTGGCTACCATAATTTCAACATCATCACCAGTTCCTAA	RFTHICGNIGYHNFNIITSS*	-2.029	9.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23040	DTGYVPRSLRTYRTATPVSA			20	SLAY-screened peptide P1390			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATACGGGTTATGTCCCTCGGAGCTTGCGGACTTACCGTACGGCCACGCCCGTCTCCGCGTAA	DTGYVPRSLRTYRTATPVSA*	-2.029	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23041	PQALTDIDSGRSIMHDNSFL			20	SLAY-screened peptide P1391			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCAGGCTCTCACTGATATCGACTCTGGTCGCAGCATTATGCACGACAACAGTTTTTTGTAA	PQALTDIDSGRSIMHDNSFL*	-2.029	6.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23042	SLM			3	SLAY-screened peptide P1392			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCTGATGTAGGCGCCCCACCACCGCCTGAACTTGATTACGCAGGATATTTGCTCCTCCTAA	SLM*APHHRLNLITQDICSS*	-2.028	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23043	VPWCTTAPCPPSGEP			15	SLAY-screened peptide P1393			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCCCTGGTGCACTACTGCTCCGTGTCCGCCTTCTGGGGAGCCTTAGCACAAGACGTATTAA	VPWCTTAPCPPSGEP*HKTY*	-2.028	0.000101	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23044	IGVLHAHASN			10	SLAY-screened peptide P1394			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGGTGTTCTCCACGCCCACGCTTCGAATTAGACCCCCGCGGACCCCTCGGACTTCTCCTAA	IGVLHAHASN*TPADPSDFS*	-2.028	0.005386	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23045	YNSYVVPCSIRKLFACHCFY			20	SLAY-screened peptide P1395			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAATTCCTACGTGGTTCCTTGTAGTATTCGTAAGCTGTTCGCGTGCCATTGCTTTTACTAA	YNSYVVPCSIRKLFACHCFY*	-2.028	0.002712	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23046	RSCAGAMRRSIGRWMRLRRR			20	SLAY-screened peptide P1396			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTCGTGCGCCGGCGCGATGCGGCGGTCGATCGGCCGCTGGATGAGGTTGCGGCGGCGTTAA	RSCAGAMRRSIGRWMRLRRR*	-2.028	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23047	YRTAVRVYTVSKTYFKTDDQ			20	SLAY-screened peptide P1397			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCGTACCGCCGTGCGGGTCTATACGGTTTCGAAGACTTATTTCAAGACTGATGATCAGTAA	YRTAVRVYTVSKTYFKTDDQ*	-2.028	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23048	AALHYANHISHFVHLDG			17	SLAY-screened peptide P1398			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCCCTTCATTACGCTAATCACATCTCGCATTTTGTTCACCTGGACGGCTAGTACATTTAA	AALHYANHISHFVHLDG*YI*	-2.027	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23049	SFDYIALPHFIVRNMRL			17	SLAY-screened peptide P1399			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTTTGACTACATTGCCCTCCCGCATTTTATTGTCCGGAATATGCGCCTCTAGAATTTCTAA	SFDYIALPHFIVRNMRL*NF*	-2.027	0.001036	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23050	EMNSTRTQWHRSSQPTNPCS			20	SLAY-screened peptide P1400			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGATGAATTCCACTCGCACGCAGTGGCACCGTTCGAGCCAGCCTACTAACCCCTGCAGTTAA	EMNSTRTQWHRSSQPTNPCS*	-2.027	9.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23051	PALYKIFKRLLHPSTTCPSA			20	SLAY-screened peptide P1401			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGCCCTCTACAAGATCTTTAAGCGTTTGTTGCATCCTTCTACCACCTGCCCGTCCGCGTAA	PALYKIFKRLLHPSTTCPSA*	-2.026	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23052	DLFGSAPWGNMYPYDYLSLRR			21	SLAY-screened peptide P1402			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCTGTTCGGTTCGGCGCCCTGGGGCAACATGTACCCCTACGATTACCTATCACTACGACGT	DLFGSAPWGNMYPYDYLSLRR	-2.026	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23053	LPKSLMQTLPSRTTHDIFIR			20	SLAY-screened peptide P1403			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCTAAGTCCCTCATGCAGACTCTCCCTAGTCGTACGACTCATGACATCTTTATTAGGTAA	LPKSLMQTLPSRTTHDIFIR*	-2.026	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23054	EPQASHPTF			9	SLAY-screened peptide P1404			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCCCCAGGCCTCGCATCCTACTTTCTAGTATTCTACGTCGTACGGTATCGGGCCTATTTAA	EPQASHPTF*YSTSYGIGPI*	-2.026	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23055	PSLIASPGEHTSPSAVWEFG			20	SLAY-screened peptide P1405			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAGTCTGATTGCCAGCCCTGGCGAGCATACCTCTCCCTCCGCCGTGTGGGAGTTCGGCTAA	PSLIASPGEHTSPSAVWEFG*	-2.026	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23056	CYELWINSQVANPSVSIRSS			20	SLAY-screened peptide P1406			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTACGAGCTGTGGATTAACTCTCAGGTCGCGAACCCGAGCGTTTCTATTCGGTCGTCGTAA	CYELWINSQVANPSVSIRSS*	-2.025	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23057	SRHNYRKRLFSDLSNGAMNP			20	SLAY-screened peptide P1407			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCGCCATAACTATCGGAAGAGGCTCTTTTCTGATCTTAGCAACGGGGCCATGAATCCTTAA	SRHNYRKRLFSDLSNGAMNP*	-2.025	0.001096	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23058	DIMFTKRSQCPCHTWTYARS			20	SLAY-screened peptide P1408			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATATTATGTTTACGAAGCGTTCGCAGTGCCCGTGTCATACGTGGACGTACGCGAGGTCTTAA	DIMFTKRSQCPCHTWTYARS*	-2.025	0.000354	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23059	TMKINASSD			9	SLAY-screened peptide P1409			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCATGAAGATTAATGCTAGCAGTGATTAGTCGGGGTTCGCTCCTCAGTTCACTTTTTAGTAA	TMKINASSD*SGFAPQFTF**	-2.023	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23060	TVLCMED			7	SLAY-screened peptide P1410			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTCCTTTGTATGGAGGATTAGACGCAGCTTTACCTGTCTCCGAATATTGTCTTGAATTAA	TVLCMED*TQLYLSPNIVLN*	-2.023	0.008676	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23061	ERSHHQTTVVYTMYLLGANT			20	SLAY-screened peptide P1411			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGAGGAGTCATCATCAGACTACCGTTGTTTATACTATGTACCTGCTTGGTGCGAATACGTAA	ERSHHQTTVVYTMYLLGANT*	-2.023	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23062	VWGEQSIFFITCGSHTNCMG			20	SLAY-screened peptide P1412			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTGGGGTGAGCAGAGTATTTTTTTTATCACGTGTGGCAGCCACACCAATTGCATGGGCTAA	VWGEQSIFFITCGSHTNCMG*	-2.023	0.001132	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23063	HFMTAACDVMAYKACVNSML			20	SLAY-screened peptide P1413			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTTCATGACTGCCGCTTGCGATGTTATGGCTTACAAGGCGTGCGTTAACAGCATGCTTTAA	HFMTAACDVMAYKACVNSML*	-2.021	0.003274	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23064	YLVRGASHNPNPHLFMRTLN			20	SLAY-screened peptide P1414			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCTGGTCCGTGGTGCTAGCCACAATCCTAACCCTCACTTGTTCATGCGCACGCTTAACTAA	YLVRGASHNPNPHLFMRTLN*	-2.02	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23065	PIYVFASAVAAMCWFVTTYS			20	SLAY-screened peptide P1415			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATTTACGTCTTCGCCAGCGCTGTGGCCGCTATGTGTTGGTTTGTTACTACCTACTCTTAA	PIYVFASAVAAMCWFVTTYS*	-2.019	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23066	FNTNRLPSVSRTNKFPTVVT			20	SLAY-screened peptide P1416			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTAACACGAATAGGCTTCCTTCTGTGTCCCGTACTAATAAGTTTCCTACCGTTGTCACCTAA	FNTNRLPSVSRTNKFPTVVT*	-2.019	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23067	NADSTVILTVIFSYGDKCGC			20	SLAY-screened peptide P1417			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGCGGACTCTACTGTTATTCTCACTGTTATTTTTTCTTATGGCGACAAGTGTGGTTGTTAA	NADSTVILTVIFSYGDKCGC*	-2.019	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23068	LITLHLIIGPFCCLSLRRRM			20	SLAY-screened peptide P1418			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATCACCCTTCACTTGATCATCGGCCCGTTTTGTTGCCTTTCCCTGAGGAGGCGCATGTAA	LITLHLIIGPFCCLSLRRRM*	-2.019	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23069	RLPNPNNPNRDPQPPARLCP			20	SLAY-screened peptide P1419			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTGCCCAATCCTAACAACCCCAACCGCGATCCTCAGCCGCCTGCGCGTCTCTGTCCGTAA	RLPNPNNPNRDPQPPARLCP*	-2.018	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23070	IQERKASCPQNHHFRFTDPL			20	SLAY-screened peptide P1420			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCAGGAGCGCAAGGCCTCCTGTCCTCAGAACCATCACTTCCGCTTTACTGACCCTCTCTAA	IQERKASCPQNHHFRFTDPL*	-2.018	0.014819	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23071	VLLMPSSTTTVSDAPTTGYM			20	SLAY-screened peptide P1421			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCTCCTTATGCCGTCCAGCACTACTACTGTCAGCGACGCGCCGACTACGGGTTACATGTAA	VLLMPSSTTTVSDAPTTGYM*	-2.017	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23072	LDLYIRVNIIPEYTGPKYTS			20	SLAY-screened peptide P1422			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGATCTCTACATCCGCGTTAATATCATTCCCGAGTATACTGGCCCTAAGTATACTTCTTAA	LDLYIRVNIIPEYTGPKYTS*	-2.017	0.010385	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23073	SYISPFNHPVFHRVATHYRS			20	SLAY-screened peptide P1423			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTACATTAGCCCGTTTAATCATCCTGTGTTTCACCGTGTCGCTACTCACTATCGCTCCTAA	SYISPFNHPVFHRVATHYRS*	-2.017	0.001817	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23074	MRLGLILRMAALLALLALRLT			21	SLAY-screened peptide P1424			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCGGTTGGGACTAATTCTTCGCATGGCAGCTCTGCTTGCCCTCCTGGCCTTACGCCTAACT	MRLGLILRMAALLALLALRLT	-2.017	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23075	RPTAVDAARRQEIYDYFNFH			20	SLAY-screened peptide P1425			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCGACTGCGGTTGATGCGGCGCGCCGTCAGGAGATCTATGACTATTTCAATTTCCACTAA	RPTAVDAARRQEIYDYFNFH*	-2.017	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23076	DTLHLGGHRGNHYIHVFFRF			20	SLAY-screened peptide P1426			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATACTCTGCACCTCGGTGGCCACCGTGGGAACCATTACATTCACGTCTTTTTTAGGTTTTAA	DTLHLGGHRGNHYIHVFFRF*	-2.016	0.001889	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23077	VPSLAVTGTNN			11	SLAY-screened peptide P1427			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCCCTCCTTGGCCGTTACGGGGACCAATAACTAGATTGCGTACCCCCCCGGCTAGCCCTAA	VPSLAVTGTNN*IAYPPG*P*	-2.016	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23078	GTGPTAANDAMCRPRHTMNA			20	SLAY-screened peptide P1428			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCACGGGCCCGACGGCCGCGAATGATGCCATGTGTCGGCCGCGGCACACTATGAACGCCTAA	GTGPTAANDAMCRPRHTMNA*	-2.016	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23079	GCDSHNRYNL			10	SLAY-screened peptide P1429			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTGTGACTCTCATAACCGCTATAACCTCTAGTATCTTTACCGTGAGGTTTGGCCCTACTAA	GCDSHNRYNL*YLYREVWPY*	-2.016	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23080	LIDLSLDLLTLIWAGRTSRRN			21	SLAY-screened peptide P1430			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATTGACCTGTCTTTGGACCTGCTCACCTTGATTTGGGCAGGAAGGACCTCACGTCGTAAC	LIDLSLDLLTLIWAGRTSRRN	-2.013	0.007873	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23081	WYNPTAAP			8	SLAY-screened peptide P1431			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTATAACCCCACTGCCGCTCCCTAGACGGCGACTCCGACTATTTGGCGGCGCATTTACTAA	WYNPTAAP*TATPTIWRRIY*	-2.013	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23082	SHTFCGQVINLHANSHTCVR			20	SLAY-screened peptide P1432			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCACACCTTTTGCGGTCAGGTCATTAACCTTCACGCTAACTCCCACACCTGTGTCCGGTAA	SHTFCGQVINLHANSHTCVR*	-2.013	2.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23083	TLGYIHYTHVEDYVRPFCNY			20	SLAY-screened peptide P1433			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTTGGGTTATATTCACTATACTCACGTCGAGGATTATGTCCGGCCTTTTTGTAATTATTAA	TLGYIHYTHVEDYVRPFCNY*	-2.012	0.000233	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23084	MAQDPRSNHRYDNKPIHYNV			20	SLAY-screened peptide P1434			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGCCCAGGATCCCAGGTCTAACCATCGCTATGACAATAAGCCTATTCACTATAATGTTTAA	MAQDPRSNHRYDNKPIHYNV*	-2.012	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23085	RDHDISSIRPSITNVNDDMQ			20	SLAY-screened peptide P1435			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGATCACGATATCTCTTCTATTCGCCCTAGCATCACTAATGTTAATGACGATATGCAGTAA	RDHDISSIRPSITNVNDDMQ*	-2.011	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23086	LVFYALIPTLSKNCSPLLSL			20	SLAY-screened peptide P1436			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGTTTTCTATGCCCTTATTCCTACCCTCAGTAAGAATTGTTCTCCTCTTCTTAGCCTTTAA	LVFYALIPTLSKNCSPLLSL*	-2.011	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23087	YRLTPFNQGLNKIIYCRFRP			20	SLAY-screened peptide P1437			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCGCCTCACTCCCTTTAATCAGGGTCTGAATAAGATTATCTACTGTCGTTTCAGGCCCTAA	YRLTPFNQGLNKIIYCRFRP*	-2.01	0.000596	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23088	SSHRDIPRPHRLLCLFPYIS			20	SLAY-screened peptide P1438			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTCCCATCGCGATATTCCGAGGCCTCACCGTCTCCTGTGTCTGTTTCCTTATATTTCCTAA	SSHRDIPRPHRLLCLFPYIS*	-2.01	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23089	ALHDAILSDCICTNPGM			17	SLAY-screened peptide P1439			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCTGCATGATGCCATTCTTAGTGATTGTATCTGTACCAATCCTGGCATGTAGAATATTTAA	ALHDAILSDCICTNPGM*NI*	-2.01	0.000978	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23090	TRCNWSILEME			11	SLAY-screened peptide P1440			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCGTTGCAATTGGAGCATCCTTGAGATGGAGTAGGCGTATGTTCCGAGTCTTCTCCCTTAA	TRCNWSILEME*AYVPSLLP*	-2.01	3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23091	TPEDAGGPHCAK			12	SLAY-screened peptide P1441			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCGGAGGACGCGGGCGGTCCTCACTGCGCCAAGTAGCCCGAGGAGATTAATCTCTCGTAA	TPEDAGGPHCAK*PEEINLS*	-2.01	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23092	PPSS			4	SLAY-screened peptide P1442			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCGTCGAGTTAGGGTTTTCATTATCCTTCGGACTCCAATGCTATTAGGATTAATTGCTAA	PPSS*GFHYPSDSNAIRINC*	-2.009	0.000438	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23093	LPTLDFPTSKGYMSDRP			17	SLAY-screened peptide P1443			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCCTACTCTTGATTTTCCGACCAGCAAGGGTTATATGTCCGATAGGCCTTAGGATTTCTAA	LPTLDFPTSKGYMSDRP*DF*	-2.009	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23094	LSLHHYSSLPVNSHWTRRAS			20	SLAY-screened peptide P1444			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAGTCTCCATCACTACTCCAGCTTGCCCGTGAATTCGCATTGGACTCGTCGGGCTTCCTAA	LSLHHYSSLPVNSHWTRRAS*	-2.009	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23095	TAPFDWSNVNTPCNTNFVYL			20	SLAY-screened peptide P1445			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGCTCCGTTCGATTGGTCTAACGTGAACACCCCTTGCAATACCAATTTTGTTTACCTTTAA	TAPFDWSNVNTPCNTNFVYL*	-2.008	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23096	LQTRLFVRREQGSIEFIYP			19	SLAY-screened peptide P1446			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCAGACTCGTCTGTTTGTTCGTAGGGAGCAGGGCTCCATTGAGTTCATCTATCCGTAGTAA	LQTRLFVRREQGSIEFIYP**	-2.008	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23097	MPLHHSTSGRLTHFLPLASK			20	SLAY-screened peptide P1447			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCCGTTGCACCATTCCACTAGCGGTAGGCTCACTCACTTCCTGCCGCTTGCGTCGAAGTAA	MPLHHSTSGRLTHFLPLASK*	-2.007	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23098	YHPKHTYRTIDTLDLPYHVT			20	SLAY-screened peptide P1448			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCACCCCAAGCATACCTACCGGACCATCGATACTCTGGATCTGCCTTATCATGTCACCTAA	YHPKHTYRTIDTLDLPYHVT*	-2.007	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23099	ATCTPLSYRL			10	SLAY-screened peptide P1449			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACCTGTACTCCCCTTTCCTACCGCCTTTAGATCAACTCTTAGCTGCTCACTCTTGGGTAA	ATCTPLSYRL*INS*LLTLG*	-2.007	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23100	PRHSVSLALASMPNIPAAYW			20	SLAY-screened peptide P1450			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGGCATAGCGTCTCCCTTGCCCTCGCTAGTATGCCCAATATTCCTGCGGCCTACTGGTAA	PRHSVSLALASMPNIPAAYW*	-2.007	0.002761	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23101	SEHFGLPPPRHILVPILDDT			20	SLAY-screened peptide P1451			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGAGCACTTCGGCCTGCCTCCGCCGAGGCACATTCTTGTCCCTATTCTCGATGACACGTAA	SEHFGLPPPRHILVPILDDT*	-2.006	0.018247	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23102	YLNNRLFCRWHGDCHI			16	SLAY-screened peptide P1452			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCTTAACAATCGTCTTTTCTGTCGCTGGCATGGTGATTGTCACATCTGACGTTCACTTAAC	YLNNRLFCRWHGDCHI*RSLN	-2.006	0.00437	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23103	NHFICFRLLLVYKLSRSGRR			20	SLAY-screened peptide P1453			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCATTTTATTTGCTTCCGCCTGCTTTTGGTTTACAAGCTTAGCCGTAGCGGCCGGCGTTAA	NHFICFRLLLVYKLSRSGRR*	-2.006	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23104	ACYYFFLFFFRSTYNRHSTD			20	SLAY-screened peptide P1454			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTGTTACTACTTTTTCCTCTTTTTCTTCAGGAGCACTTACAACCGCCATAGTACTGATTAA	ACYYFFLFFFRSTYNRHSTD*	-2.006	0.00043	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23105	PPKCDHTRLWALYNDPILIL			20	SLAY-screened peptide P1455			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCAAGTGCGATCACACGCGTCTTTGGGCTTTGTATAACGACCCTATCTTGATTCTTTAA	PPKCDHTRLWALYNDPILIL*	-2.005	0.00055	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23106	FYPIHHTWYTQLRTCYDSAP			20	SLAY-screened peptide P1456			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTATCCTATCCACCACACCTGGTATACTCAGCTTCGCACCTGTTATGATAGTGCCCCCTAA	FYPIHHTWYTQLRTCYDSAP*	-2.005	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23107	FRMNSFDHYASIIPTTRGTI			20	SLAY-screened peptide P1457			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCGGATGAACAGCTTTGATCACTATGCGTCTATTATCCCGACGACGCGCGGGACGATTTAA	FRMNSFDHYASIIPTTRGTI*	-2.005	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23108	PRALWHASDLHSG			13	SLAY-screened peptide P1458			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGCGCTCTTTGGCATGCCAGTGATCTTCATTCTGGTTAGACCCCCACTAAGGTTGGCTAA	PRALWHASDLHSG*TPTKVG*	-2.004	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23109	SPPIRFSCYLPDSTCSDTTS			20	SLAY-screened peptide P1459			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCCTCCCATTCGTTTTTCTTGTTACCTTCCCGATTCCACCTGCAGTGACACCACTTCGTAA	SPPIRFSCYLPDSTCSDTTS*	-2.004	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23110	SCIFPKSPIQSYASTDLSIS			20	SLAY-screened peptide P1460			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTGCATCTTCCCTAAGAGCCCGATCCAGTCGTATGCTAGCACGGATTTGTCGATTTCCTAA	SCIFPKSPIQSYASTDLSIS*	-2.004	0.000201	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23111	RLPRCHTMYLRHSLSHMQIR			20	SLAY-screened peptide P1461			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTCCCTCGCTGTCATACGATGTATCTCCGTCATTCCTTGAGTCACATGCAGATTCGCTAA	RLPRCHTMYLRHSLSHMQIR*	-2.004	0.001553	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23112	NCA			3	SLAY-screened peptide P1462			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTGTGCTTAGTGCTCGTATAATACTATCCATAACAAGACCTAGAATCTGTTGGGTGTGTAA	NCA*CSYNTIHNKT*NLLGV*	-2.003	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23113	RQHGYCHFLRYSRHRRMHRH			20	SLAY-screened peptide P1463			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCAGCATGGTTATTGTCATTTCCTTCGTTATTCCCGCCATCGTCGTATGCACCGTCATTAA	RQHGYCHFLRYSRHRRMHRH*	-2.003	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23114	DHHWLTALVNPHGMVSPNRL			20	SLAY-screened peptide P1464			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCACCACTGGCTCACCGCTCTGGTCAATCCGCACGGCATGGTGTCTCCTAATAGGCTCTAA	DHHWLTALVNPHGMVSPNRL*	-2.002	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23115	RAGAIPHIARALALFWISSS			20	SLAY-screened peptide P1465			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCCGGCGCGATTCCCCATATCGCGCGCGCTCTCGCTTTGTTTTGGATCTCTTCTTCGTAA	RAGAIPHIARALALFWISSS*	-2.002	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23116	SYIVKSYLHNRSSATCKTYR			20	SLAY-screened peptide P1466			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTATATTGTCAAGTCTTATCTGCATAATCGGAGTTCGGCTACTTGTAAGACTTACAGGTAA	SYIVKSYLHNRSSATCKTYR*	-2.002	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23117	ALVYSNRPAIHDGEPLITNT			20	SLAY-screened peptide P1467			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCTCGTCTATAGCAATAGGCCTGCGATTCACGACGGGGAGCCGCTGATTACTAATACCTAA	ALVYSNRPAIHDGEPLITNT*	-2.001	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23118	LSSLVANAITHCFLLAREIR			20	SLAY-screened peptide P1468			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCGAGTCTCGTTGCTAATGCGATTACCCACTGCTTCTTGCTGGCGCGCGAGATTCGTTAA	LSSLVANAITHCFLLAREIR*	-2.001	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23119	FPRYMFTATGRLDDGRAMSY			20	SLAY-screened peptide P1469			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCCCAGGTATATGTTTACTGCTACTGGCCGCCTTGATGACGGCCGGGCCATGAGTTATTAA	FPRYMFTATGRLDDGRAMSY*	-2.0	0.008938	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23120	LVATCSPYHTPTRLEQHNTS			20	SLAY-screened peptide P1470			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGTGGCTACGTGCTCCCCTTATCATACTCCTACGCGTCTGGAGCAGCATAACACTAGTTAA	LVATCSPYHTPTRLEQHNTS*	-2.0	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23121	ADDTRPRRTITRLLVYAFHT			20	SLAY-screened peptide P1471			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGACGACACTCGCCCCAGGCGTACTATTACCAGGCTTCTTGTCTATGCTTTCCACACGTAA	ADDTRPRRTITRLLVYAFHT*	-2.0	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23122	FSSHGHQLEAKFIFRPKPNS			20	SLAY-screened peptide P1472			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAGTTCTCACGGCCATCAGCTTGAGGCCAAGTTCATTTTCAGGCCCAAGCCTAACTCCTAA	FSSHGHQLEAKFIFRPKPNS*	-1.999	0.000233	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23123	GHYYLCFYLAVTLTIRRIDLT			21	SLAY-screened peptide P1473			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCACTATTATCTCTGTTTCTACCTTGCAGTGACATTGACCATAAGAAGAATAGATCTAACT	GHYYLCFYLAVTLTIRRIDLT	-1.998	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23124	PLPNLVGTAASNHIFEKSKST			21	SLAY-screened peptide P1474			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTGCCTAACCTGGTTGGGACCGCTGCGAGTAATCATATTTTTGAGAAGAGTAAGTCGACC	PLPNLVGTAASNHIFEKSKST	-1.997	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23125	HSSIWNRCHIFMHTLDHYPT			20	SLAY-screened peptide P1475			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAGCTCTATCTGGAACCGCTGCCATATTTTTATGCATACGCTTGACCATTACCCCACCTAA	HSSIWNRCHIFMHTLDHYPT*	-1.997	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23126	INSRRDTGDSRLVFVAYSSP			20	SLAY-screened peptide P1476			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAATTCTCGTAGGGATACTGGCGATTCCCGTTTGGTGTTTGTTGCTTACTCGTCTCCTTAA	INSRRDTGDSRLVFVAYSSP*	-1.997	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23127	LYGM			4	SLAY-screened peptide P1477			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTACGGGATGTAGTAGCGCCCTCTTGTGGGGTTTATCTATTACTCTAGTCATTAGAAGTAA	LYGM**RPLVGFIYYSSH*K*	-1.996	0.000222	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23128	ILNWIKGYLHHVLARRNLSS			20	SLAY-screened peptide P1478			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCTGAACTGGATCAAGGGTTATCTTCACCACGTGTTGGCCCGTCGGAATCTTTCGTCTTAA	ILNWIKGYLHHVLARRNLSS*	-1.996	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23129	NGLDPCYNNCINYLNHIR			18	SLAY-screened peptide P1479			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGGGCTTGATCCCTGCTATAATAACTGTATCAACTATCTCAATCATATCCGGTAGACTTAA	NGLDPCYNNCINYLNHIR*T*	-1.996	0.000577	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23130	RPCDFLLSNKSVVFQNKNRF			20	SLAY-screened peptide P1480			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCTTGTGATTTTTTGCTTTCTAACAAGTCTGTTGTGTTTCAGAATAAGAACCGGTTTTAA	RPCDFLLSNKSVVFQNKNRF*	-1.995	0.000375	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23131	RRHPSVLYPCTFQIVYYGLC			20	SLAY-screened peptide P1481			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAGGCACCCCTCGGTCCTGTACCCCTGCACTTTTCAGATTGTCTACTATGGCCTTTGTTAA	RRHPSVLYPCTFQIVYYGLC*	-1.995	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23132	DCRSCNYRPTGSWRECGVIP			20	SLAY-screened peptide P1482			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTGTCGCTCCTGTAATTACCGTCCCACGGGGTCCTGGCGCGAGTGTGGTGTCATCCCTTAA	DCRSCNYRPTGSWRECGVIP*	-1.995	0.000754	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23133	PMYTSPFSAYDRQHNTNCCR			20	SLAY-screened peptide P1483			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATGTACACGAGTCCTTTCTCGGCGTATGACCGTCAGCACAACACTAATTGTTGTCGTTAA	PMYTSPFSAYDRQHNTNCCR*	-1.995	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23134	LVLGCCLCCRIRFRATINSN			20	SLAY-screened peptide P1484			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGTCCTTGGGTGCTGTTTGTGCTGCCGTATCCGCTTTCGGGCCACTATCAATTCGAACTAA	LVLGCCLCCRIRFRATINSN*	-1.994	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23135	DNVDNN			6	SLAY-screened peptide P1485			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATAACGTGGATAATAATTAGATTCGCCCTATCGCGACAATGTCAATTACCGTATTCCTTAAC	DNVDNN*IRPIATMSITVFLN	-1.994	0.005993	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23136	KGYLGRFRPRDTLNDRKTAR			20	SLAY-screened peptide P1486			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGGGTTACCTGGGCCGGTTCCGTCCTAGGGACACTCTCAACGACCGCAAGACTGCGCGGTAA	KGYLGRFRPRDTLNDRKTAR*	-1.994	0.006092	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23137	SFPSSFLPYVLLDRVERNCS			20	SLAY-screened peptide P1487			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTTCCCTTCCAGCTTCTTGCCCTATGTCCTGCTGGACCGTGTCGAGCGGAATTGCTCCTAA	SFPSSFLPYVLLDRVERNCS*	-1.993	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23138	PNNYSDSFMAHSHLLDEILN			20	SLAY-screened peptide P1488			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAATAACTATAGCGATAGTTTCATGGCCCACTCTCATTTGCTGGACGAGATCCTCAATTAA	PNNYSDSFMAHSHLLDEILN*	-1.992	0.002024	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23139	LSQAADRASSCTNGVPLPSQ			20	SLAY-screened peptide P1489			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCCCAGGCTGCCGATCGGGCTTCCAGTTGTACTAATGGTGTGCCTCTGCCTAGCCAGTAA	LSQAADRASSCTNGVPLPSQ*	-1.992	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23140	LVDVEFTWLNVSESIILETT			20	SLAY-screened peptide P1490			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGGTCGATGTGGAGTTCACGTGGCTTAATGTTAGTGAGTCCATCATTCTTGAGACCACCTAA	LVDVEFTWLNVSESIILETT*	-1.992	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23141	LVIQIGLVSWSPRRRSRILRN			21	SLAY-screened peptide P1491			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGTGATTCAGATAGGCCTGGTGTCGTGGTCACCCCGGCGCCGGAGTCGAATACTCCGTAAC	LVIQIGLVSWSPRRRSRILRN	-1.991	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23142	PRTKFTLNVDHASDHGESPL			20	SLAY-screened peptide P1492			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGGACCAAGTTCACTCTCAATGTCGATCATGCGAGCGACCACGGCGAGAGTCCGTTGTAA	PRTKFTLNVDHASDHGESPL*	-1.99	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23143	TLVNRHVGKADSGSRPHMYA			20	SLAY-screened peptide P1493			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTGGTGAATCGTCATGTTGGCAAGGCTGATAGCGGTAGTCGCCCTCACATGTACGCGTAA	TLVNRHVGKADSGSRPHMYA*	-1.989	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23144	RARVPLWDHLSNSPAHNDYT			20	SLAY-screened peptide P1494			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCTCGCGTGCCCCTCTGGGATCATCTTTCTAATTCCCCGGCTCATAACGACTATACTTAA	RARVPLWDHLSNSPAHNDYT*	-1.988	3.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23145	KCSTMSSSRG			10	SLAY-screened peptide P1495			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGTGCTCGACCATGTCTAGCTCCCGGGGCTAGAGTCTTTTGTACTTGTAGACTAATAGCTAA	KCSTMSSSRG*SLLYL*TNS*	-1.988	2.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23146	VTTTHQR			7	SLAY-screened peptide P1496			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTACCACTACCCACCAGCGCTAGCTTTCTATCAGTAAGCCGATTTATTCTGCGACGGCTTAA	VTTTHQR*LSISKPIYSATA*	-1.987	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23147	GANYRRENCSQFSSLQAVNR			20	SLAY-screened peptide P1497			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGCTAACTACCGGAGGGAGAATTGTTCTCAGTTTTCTAGTCTTCAGGCTGTGAATCGGTAA	GANYRRENCSQFSSLQAVNR*	-1.987	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23148	PYSLAPSFPLVQCYDNSRPL			20	SLAY-screened peptide P1498			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTACAGTCTGGCTCCGAGCTTCCCTTTGGTCCAGTGCTATGACAATAGCAGGCCGTTGTAA	PYSLAPSFPLVQCYDNSRPL*	-1.987	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23149	SYPTGLAFFAVVAIITRTGRN			21	SLAY-screened peptide P1499			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTACCCTACGGGTCTCGCTTTTTTCGCAGTTGTTGCGATTATAACGAGAACCGGCCGTAAC	SYPTGLAFFAVVAIITRTGRN	-1.986	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23150	LHHPQKAHSAPAR			13	SLAY-screened peptide P1500			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCATCACCCCCAGAAGGCGCATTCGGCTCCCGCTCGCTAGGCCGCTGCGGCCAGCCACTAA	LHHPQKAHSAPAR*AAAASH*	-1.985	0.001863	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23151	EPFPFALSQPPLLHGQVKAR			20	SLAY-screened peptide P1501			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCCCTTCCCTTTTGCTCTTAGCCAGCCCCCTCTCCTCCACGGTCAGGTTAAGGCGAGGTAA	EPFPFALSQPPLLHGQVKAR*	-1.985	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23152	KRNKVN			6	SLAY-screened peptide P1502			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCGGAATAAGGTTAATTAGGGTGCCACGCCCGGCGCTTCGAGTCTGGCCACCGCGCTTTAA	KRNKVN*GATPGASSLATAL*	-1.985	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23153	GKRLSRTLLAMSLVAV			16	SLAY-screened peptide P1503			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCAAGCGGTTGTCGCGCACGCTCCTAGCCATGAGTTTGGTTGCCGTATGAGCACCCGGTAAC	GKRLSRTLLAMSLVAV*APGN	-1.985	0.006057	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23154	PILSPDRSRCRLLPVQAYGL			20	SLAY-screened peptide P1504			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATTCTTTCCCCCGACCGGAGTAGGTGCCGTTTGCTGCCCGTGCAGGCCTATGGGCTGTAA	PILSPDRSRCRLLPVQAYGL*	-1.985	0.001959	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23155	SYIVYFLPVRRTPARTLGLLN			21	SLAY-screened peptide P1505			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTACATTGTGTATTTCCTCCCCGTGCGCCGTACACCTGCCCGTACACTCGGGTTGCTTAAC	SYIVYFLPVRRTPARTLGLLN	-1.984	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23156	YSVY			4	SLAY-screened peptide P1506			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTCCGTCTACTAGATTACTGCGTTTAAGTATTTGCCTTACTTTCTGGGGACCCCCCATTAA	YSVY*ITAFKYLPYFLGTPH*	-1.983	5.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23157	HRTRMLFFISARRNSVFIHN			20	SLAY-screened peptide P1507			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCGCACTAGGATGCTTTTTTTCATCTCTGCGAGGAGGAACTCTGTCTTCATCCACAATTAA	HRTRMLFFISARRNSVFIHN*	-1.983	0.005663	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23158	HILQASTWLDAVNIGIFTRW			20	SLAY-screened peptide P1508			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATATTCTGCAGGCCTCCACTTGGCTGGATGCCGTTAACATCGGTATTTTCACCCGTTGGTAA	HILQASTWLDAVNIGIFTRW*	-1.982	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23159	PILVDHFLHMHKTDTCHKSR			20	SLAY-screened peptide P1509			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATTCTCGTTGATCATTTTCTCCACATGCACAAGACCGACACCTGCCACAAGTCTCGTTAA	PILVDHFLHMHKTDTCHKSR*	-1.982	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23160	CIDARKCDCVNDRNVCTVFC			20	SLAY-screened peptide P1510			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCATTGACGCCCGTAAGTGTGATTGTGTTAATGACCGGAACGTTTGCACGGTGTTTTGTTAA	CIDARKCDCVNDRNVCTVFC*	-1.981	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23161	TAHPALASLLY			11	SLAY-screened peptide P1511			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGCCCATCCTGCGCTTGCGAGCCTCCTTTATTAGGGCCTTTCCTATACGACGGCCTGCTAA	TAHPALASLLY*GLSYTTAC*	-1.981	0.000375	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23162	MVCLTYTSKPIVSKPLPN			18	SLAY-screened peptide P1512			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGTTTGTCTGACGTACACCTCGAAGCCTATTGTTTCTAAGCCTCTGCCGAACTAGGACTAA	MVCLTYTSKPIVSKPLPN*D*	-1.981	0.000366	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23163	SVAQDFFHDFTTAPFCFSII			20	SLAY-screened peptide P1513			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGTTGCTCAGGATTTTTTCCACGACTTCACCACTGCGCCGTTCTGCTTTAGTATTATTTAA	SVAQDFFHDFTTAPFCFSII*	-1.98	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23164	FQQFYTSGCNTPYYQLIKDY			20	SLAY-screened peptide P1514			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCAGCAGTTCTATACTTCGGGCTGCAACACTCCGTATTATCAGCTGATTAAGGACTACTAA	FQQFYTSGCNTPYYQLIKDY*	-1.979	0.00016	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23165	NHLHKHPFINGRPGAQGTPP			20	SLAY-screened peptide P1515			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCATCTGCATAAGCACCCGTTCATCAACGGCCGGCCCGGTGCGCAGGGCACGCCGCCTTAA	NHLHKHPFINGRPGAQGTPP*	-1.979	0.000704	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23166	GAMACQCPPYTTSPCLMSNA			20	SLAY-screened peptide P1516			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGCTATGGCTTGTCAGTGCCCCCCCTATACTACTAGTCCCTGCCTTATGTCGAACGCTTAA	GAMACQCPPYTTSPCLMSNA*	-1.979	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23167	ANKRGNPCQRPNATG			15	SLAY-screened peptide P1517			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTAATAAGCGGGGTAACCCTTGTCAGCGCCCGAATGCGACTGGTTAGGTTTGTCCCCGTTAA	ANKRGNPCQRPNATG*VCPR*	-1.979	5.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23168	ARREYPEPTNTCSPAVLLPM			20	SLAY-screened peptide P1518			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCGCCGTGAGTATCCCGAGCCTACGAATACGTGTAGTCCCGCTGTCTTGCTTCCCATGTAA	ARREYPEPTNTCSPAVLLPM*	-1.978	3.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23169	SSSHTCSTANLILQPSAITT			20	SLAY-screened peptide P1519			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAGCTCTCACACGTGCAGTACTGCTAACTTGATTCTGCAGCCCAGTGCCATTACTACGTAA	SSSHTCSTANLILQPSAITT*	-1.978	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23170	CHPSSPTFPFIIPVGSDAYT			20	SLAY-screened peptide P1520			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCACCCTAGTTCGCCTACCTTCCCTTTTATTATCCCTGTGGGTAGCGACGCCTATACCTAA	CHPSSPTFPFIIPVGSDAYT*	-1.977	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23171	NRLFCGDPKDCLWRESSSYE			20	SLAY-screened peptide P1521			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCGCCTGTTTTGTGGGGACCCCAAGGACTGCCTTTGGCGGGAGAGCTCGTCGTATGAGTAA	NRLFCGDPKDCLWRESSSYE*	-1.977	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23172	IGVHICDLAFSRMAMRELFC			20	SLAY-screened peptide P1522			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGGTGTCCATATTTGCGATCTTGCCTTCAGTCGCATGGCTATGCGTGAGCTTTTCTGTTAA	IGVHICDLAFSRMAMRELFC*	-1.977	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23173	RWAEPITPQSSFLTAAHPLS			20	SLAY-screened peptide P1523			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTGGGCCGAGCCGATTACGCCCCAGTCCAGTTTTCTCACGGCTGCCCACCCGCTTTCCTAA	RWAEPITPQSSFLTAAHPLS*	-1.974	0.001933	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23174	LAFLHVGIGCHVTDPCYSDF			20	SLAY-screened peptide P1524			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGCTTTCTTGCACGTTGGCATCGGTTGCCATGTTACGGATCCTTGTTATAGTGATTTCTAA	LAFLHVGIGCHVTDPCYSDF*	-1.973	0.005717	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23175	RQCNHGNNCGEFLVHCTSFA			20	SLAY-screened peptide P1525			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCAGTGCAATCACGGCAACAATTGCGGTGAGTTCCTTGTGCACTGTACGAGCTTCGCGTAA	RQCNHGNNCGEFLVHCTSFA*	-1.972	0.000298	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23176	SYIYPH			6	SLAY-screened peptide P1526			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTACATCTATCCCCATTAGTGTTGGCGGCTTCGCTGTATCTACTACGCTCTTAATCATTAA	SYIYPH*CWRLRCIYYALNH*	-1.971	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23177	YLLPFTVLGPRRRVTPFIRK			20	SLAY-screened peptide P1527			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCTCCTGCCGTTCACGGTGCTTGGGCCTCGCCGGCGTGTTACTCCTTTCATCCGCAAGTAA	YLLPFTVLGPRRRVTPFIRK*	-1.971	0.002202	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23178	VGTLTRAKAYRVYCCPNRRSN			21	SLAY-screened peptide P1528			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGGCACCCTGACTCGGGCGAAGGCTTATCGGGTCTATTGCTGTCCCAACCGGAGAAGTAAC	VGTLTRAKAYRVYCCPNRRSN	-1.971	0.000159	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23179	RLCTHPCDRFGDASGRSAVN			20	SLAY-screened peptide P1529			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTTTGCACGCATCCCTGCGACCGTTTTGGCGATGCGTCGGGCCGGTCCGCCGTGAATTAA	RLCTHPCDRFGDASGRSAVN*	-1.971	0.004502	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23180	STACFDDAGDKFIFRMTLLVN			21	SLAY-screened peptide P1530			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCACTGCTTGTTTTGATGATGCCGGGGATAAATTTATTTTTAGAATGACTCTTTTAGTTAAC	STACFDDAGDKFIFRMTLLVN	-1.97	0.000395	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23181	HWSPLLLNSFDLDHHYLFYI			20	SLAY-screened peptide P1531			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTGGTCGCCTCTTCTGCTCAATAGTTTCGACCTGGACCATCATTATCTCTTTTATATCTAA	HWSPLLLNSFDLDHHYLFYI*	-1.97	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23182	SSCAVCAWQYRTCSDCSVTL			20	SLAY-screened peptide P1532			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTCCTGTGCGGTCTGCGCTTGGCAGTACCGTACCTGTTCCGACTGCAGCGTCACCCTCTAA	SSCAVCAWQYRTCSDCSVTL*	-1.97	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23183	DRPTVFPCYLTQVLTSYVVA			20	SLAY-screened peptide P1533			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCGCCCCACCGTCTTCCCCTGCTACCTTACTCAGGTTCTCACCTCGTATGTCGTTGCGTAA	DRPTVFPCYLTQVLTSYVVA*	-1.97	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23184	TDHGPNHQPF			10	SLAY-screened peptide P1534			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGACCATGGTCCCAACCATCAGCCCTTTTAGCTTGGCGTTACTGCTAATCCCTATGACTAA	TDHGPNHQPF*LGVTANPYD*	-1.969	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23185	GARLVLFYPSTTKDQNAPFI			20	SLAY-screened peptide P1535			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGGCTCGCCTCGTGCTTTTTTATCCCAGTACGACCAAGGACCAGAACGCGCCCTTTATTTAA	GARLVLFYPSTTKDQNAPFI*	-1.968	0.000292	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23186	RPDYALYHKPSDRANWVHYW			20	SLAY-screened peptide P1536			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCTGACTACGCGCTCTATCATAAGCCTTCTGATCGCGCTAATTGGGTCCATTACTGGTAA	RPDYALYHKPSDRANWVHYW*	-1.968	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23187	HILEFQASHMYVWSNAGSSY			20	SLAY-screened peptide P1537			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATATTCTGGAGTTTCAGGCGAGCCACATGTATGTTTGGTCTAACGCCGGTAGTAGTTATTAA	HILEFQASHMYVWSNAGSSY*	-1.967	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23188	PSTSSGYGFDCRSLFSFNWT			20	SLAY-screened peptide P1538			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTCCACCTCCTCCGGTTATGGGTTTGATTGCAGGAGCCTTTTTAGTTTTAATTGGACGTAA	PSTSSGYGFDCRSLFSFNWT*	-1.967	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23189	TAVPSPRVTVDKIPTTHVIR			20	SLAY-screened peptide P1539			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGCCGTTCCTTCCCCTAGGGTTACTGTCGACAAGATTCCTACCACCCATGTCATCCGCTAA	TAVPSPRVTVDKIPTTHVIR*	-1.967	7.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23190	TLVPHYSQDTMRCTLFSHLI			20	SLAY-screened peptide P1540			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTTGGTCCCCCATTATTCTCAGGACACCATGAGGTGCACCCTCTTTAGTCACTTGATTTAA	TLVPHYSQDTMRCTLFSHLI*	-1.967	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23191	WSYASLTAIPFAVL			14	SLAY-screened peptide P1541			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGAGCTACGCGTCGTTGACCGCCATTCCGTTTGCAGTACTGTGACGACTAGCTAACTGAGTA	WSYASLTAIPFAVL*RLAN*V	-1.966	0.005069	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23192	WPVHVNSPTCYEVPLNRQLS			20	SLAY-screened peptide P1542			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCCGGTCCACGTCAATAGTCCGACGTGCTACGAGGTCCCCCTTAACAGGCAGCTGAGTTAA	WPVHVNSPTCYEVPLNRQLS*	-1.966	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23193	LPAGTIAFQYENSSYIVILY			20	SLAY-screened peptide P1543			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCCGCGGGCACCATCGCGTTCCAGTATGAGAACTCCTCCTATATTGTTATTTTGTATTAA	LPAGTIAFQYENSSYIVILY*	-1.966	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23194	TAPRQNRSVSLR			12	SLAY-screened peptide P1544			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGCCCCCCGGCAGAACAGGAGCGTCTCTCTCCGTTAGGTTAACCTGCCGGCTCCGAACTAA	TAPRQNRSVSLR*VNLPAPN*	-1.966	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23195	FNSLPYRQLATFCIVVPRSV			20	SLAY-screened peptide P1545			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAACTCTCTTCCCTACCGTCAGCTGGCGACTTTTTGCATCGTGGTTCCGCGCTCTGTGTAA	FNSLPYRQLATFCIVVPRSV*	-1.965	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23196	PSFF			4	SLAY-screened peptide P1546			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGCTTTTTTTAGACTTACGGGGCGTGCAATAATGACCATAGCTCCCTTCTGGTCCCCTAA	PSFF*TYGACNNDHSSLLVP*	-1.965	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23197	SIYHDTPRYGTASYALLGLS			20	SLAY-screened peptide P1547			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCATTTATCACGACACTCCGAGGTATGGGACGGCTTCTTACGCGTTGCTCGGCTTGAGTTAA	SIYHDTPRYGTASYALLGLS*	-1.965	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23198	FYEGRQSMPSCYYPIVSLLT			20	SLAY-screened peptide P1548			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTACGAGGGTCGTCAGAGCATGCCCAGCTGTTATTACCCTATTGTCAGCCTCCTTACGTAA	FYEGRQSMPSCYYPIVSLLT*	-1.964	0.002233	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23199	PPTSLPYLCSPLNPVYLQPL			20	SLAY-screened peptide P1549			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCTACCTCTCTGCCCTACCTTTGCAGTCCTCTGAATCCGGTCTATCTCCAGCCCCTTTAA	PPTSLPYLCSPLNPVYLQPL*	-1.964	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23200	SYTFRARTAGRDCSRCGPPL			20	SLAY-screened peptide P1550			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTATACTTTTCGTGCTAGGACTGCTGGTCGGGACTGTTCCCGCTGCGGCCCTCCTCTGTAA	SYTFRARTAGRDCSRCGPPL*	-1.963	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23201	AASKRKPPHSCGVAGSCACY			20	SLAY-screened peptide P1551			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCTAGCAAGCGCAAGCCTCCGCATTCTTGTGGTGTTGCTGGCTCTTGCGCTTGTTACTAA	AASKRKPPHSCGVAGSCACY*	-1.963	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23202	SALLTHARAYANRVAAATEIN			21	SLAY-screened peptide P1552			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGCTCTTCTTACTCATGCTCGTGCGTACGCCAATCGCGTTGCCGCTGCCACTGAGATTAAC	SALLTHARAYANRVAAATEIN	-1.963	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23203	SYSTMFLSLLFALETANMKC			20	SLAY-screened peptide P1553			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTATAGTACCATGTTCCTGTCGTTGCTTTTTGCGCTGGAGACCGCTAACATGAAGTGTTAA	SYSTMFLSLLFALETANMKC*	-1.962	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23204	TRHIPSIKQCAVFHSRLPGY			20	SLAY-screened peptide P1554			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAGGCACATCCCCTCGATCAAGCAGTGTGCGGTTTTTCACAGCAGGCTCCCCGGTTATTAA	TRHIPSIKQCAVFHSRLPGY*	-1.961	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23205	CSSAGDFFTNPHNINPKSII			20	SLAY-screened peptide P1555			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTCCTCGGCCGGTGATTTTTTCACTAATCCGCATAATATCAACCCCAAGAGCATCATTTAA	CSSAGDFFTNPHNINPKSII*	-1.961	6.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23206	PRTCS			5	SLAY-screened peptide P1556			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGTACGTGTTCTTAGAATCTGCGGTCTAGCACTAACGCTTTCGCTATTATTCATATTTAA	PRTCS*NLRSSTNAFAIIHI*	-1.961	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23207	TFLIILLLLSPTSWSLLPPIN			21	SLAY-screened peptide P1557			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTTTCTTATCATACTGCTACTGCTGTCTCCTACATCATGGTCACTTCTACCGCCAATTAAC	TFLIILLLLSPTSWSLLPPIN	-1.961	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23208	RPSAPIFCLSIHKDVKKQLC			20	SLAY-screened peptide P1558			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCCAGCGCCCCTATCTTTTGTTTGTCCATTCATAAGGATGTCAAGAAGCAGCTTTGCTAA	RPSAPIFCLSIHKDVKKQLC*	-1.961	0.00011	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23209	SDIHAPKRILQTSNTL			16	SLAY-screened peptide P1559			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGATATTCACGCTCCCAAGAGGATCCTTCAGACGAGTAACACCCTGTAGTACAATTTTTAC	SDIHAPKRILQTSNTL*YNFY	-1.96	2.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23210	VKPRPLHDRP			10	SLAY-screened peptide P1560			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTAAACCTCGGCCATTGCATGATCGACCATAACTCGCACGGCCACAACACCAAGTACTAACT	VKPRPLHDRP*LARPQHQVLT	-1.96	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23211	NGCTSHNYFSRVQDPLQPIR			20	SLAY-screened peptide P1561			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGGCTGCACCTCCCACAACTACTTTTCGCGTGTGCAGGACCCCTTGCAGCCTATCCGCTAA	NGCTSHNYFSRVQDPLQPIR*	-1.96	0.001464	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23212	HSTRGACVLTYSPYGTHINP			20	SLAY-screened peptide P1562			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTCGACGCGCGGTGCTTGTGTTCTCACTTACAGCCCGTACGGTACCCATATCAATCCGTAA	HSTRGACVLTYSPYGTHINP*	-1.959	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23213	PGFWSFTPSDYDCHWTHFYL			20	SLAY-screened peptide P1563			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGGTTTTTGGAGCTTCACCCCCTCGGATTATGATTGCCACTGGACGCATTTCTACCTTTAA	PGFWSFTPSDYDCHWTHFYL*	-1.959	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23214	TVTAPWNPPITNKLLTFPTG			20	SLAY-screened peptide P1564			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTGACCGCCCCCTGGAATCCCCCTATTACGAATAAGCTTCTCACCTTCCCCACGGGCTAA	TVTAPWNPPITNKLLTFPTG*	-1.958	0.011425	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23215	PLQASHLSRSPFTCLHP			17	SLAY-screened peptide P1565			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTGCAGGCTAGTCATCTCAGCAGGTCCCCCTTTACTTGCCTCCACCCTTAGTTTCATTAA	PLQASHLSRSPFTCLHP*FH*	-1.958	0.004853	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23216	HPKYLSRTTHGSISHQYPTA			20	SLAY-screened peptide P1566			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCTAAGTACCTTTCTCGGACTACTCACGGTTCTATCTCCCATCAGTACCCTACTGCCTAA	HPKYLSRTTHGSISHQYPTA*	-1.958	0.000159	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23217	GLKNAANESRDPMEYTLSCH			20	SLAY-screened peptide P1567			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCTTAAGAATGCTGCGAATGAGAGTAGGGACCCTATGGAGTATACTCTCTCGTGCCATTAA	GLKNAANESRDPMEYTLSCH*	-1.958	0.002337	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23218	RYLSSFWRSLARFWKRYNNC			20	SLAY-screened peptide P1568			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTACCTTTCCAGTTTTTGGCGCTCTTTGGCCCGGTTTTGGAAGCGTTATAATAACTGCTAA	RYLSSFWRSLARFWKRYNNC*	-1.957	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23219	RPIRLNPRCYDRPNCSRHWS			20	SLAY-screened peptide P1569			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCCATCCGGCTTAACCCTAGGTGCTACGATAGGCCTAATTGTAGCCGCCACTGGTCCTAA	RPIRLNPRCYDRPNCSRHWS*	-1.957	0.0092	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23220	RWTSHAHRRYIPINP			15	SLAY-screened peptide P1570			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGGACTTCCCATGCTCACCGTCGTTATATTCCCATCAACCCGTAGATTTATGTCTTTTAA	RWTSHAHRRYIPINP*IYVF*	-1.957	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23221	HVSRYRALL			9	SLAY-screened peptide P1571			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGTCTCCCGGTATCGTGCCCTCTTGTAGCCCGGTAGTTACACGTCCGTGTTTCGGCCCTAA	HVSRYRALL*PGSYTSVFRP*	-1.957	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23222	STPLALLRTCSTGNHQSFRQ			20	SLAY-screened peptide P1572			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCACTCCTCTCGCTTTGTTGCGTACCTGTAGCACGGGGAATCACCAGTCGTTTCGGCAGTAA	STPLALLRTCSTGNHQSFRQ*	-1.956	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23223	YVPMPVSLRTRRRGRPI			17	SLAY-screened peptide P1573			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGTTCCTATGCCCGTTTCTCTCCGTACGAGGAGGCGCGGTCGGCCTATCTAGGCTCCTTAA	YVPMPVSLRTRRRGRPI*AP*	-1.955	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23224	PPGSLDQVNPLSHCMCYIFS			20	SLAY-screened peptide P1574			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTGGCTCTCTCGACCAGGTCAACCCTTTGTCTCATTGTATGTGCTATATTTTTTCCTAA	PPGSLDQVNPLSHCMCYIFS*	-1.955	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23225	WLLHKSVPLRAVVTVSHNMF			20	SLAY-screened peptide P1575			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCTGCTTCATAAGAGCGTGCCGCTCAGGGCTGTCGTTACTGTGTCGCACAATATGTTCTAA	WLLHKSVPLRAVVTVSHNMF*	-1.955	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23226	PRICPLTINTHLAF			14	SLAY-screened peptide P1576			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGCATTTGTCCTCTCACCATCAACACGCATCTTGCTTTTTGAATACTTAGCTTGGGTAAC	PRICPLTINTHLAF*ILSLGN	-1.955	9.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23227	PAATSGFKPMSHLLPEICTL			20	SLAY-screened peptide P1577			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCTGCGACGAGCGGTTTTAAGCCTATGAGCCATCTCCTCCCCGAGATTTGCACCCTGTAA	PAATSGFKPMSHLLPEICTL*	-1.954	3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23228	PPDWG			5	SLAY-screened peptide P1578			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTGATTGGGGCTAGTCTGCGATCCTGTTTGGTCTCCATGATACTCATGCTCCCCACTAA	PPDWG*SAILFGLHDTHAPH*	-1.953	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23229	YPIWYMHPGGPQAYSDIKYM			20	SLAY-screened peptide P1579			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCCCATTTGGTATATGCACCCGGGGGGCCCGCAGGCCTATAGTGACATTAAGTATATGTAA	YPIWYMHPGGPQAYSDIKYM*	-1.952	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23230	QGACLCPCMTHGYMYDLILL			20	SLAY-screened peptide P1580			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGGCGCGTGCCTTTGCCCGTGCATGACCCACGGCTACATGTATGACTTGATCCTTCTCTAA	QGACLCPCMTHGYMYDLILL*	-1.952	0.00529	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23231	PFHSYHNTIYAS			12	SLAY-screened peptide P1581			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTCCACAGCTATCACAATACTATTTATGCCTCGTAGACGATGTCCACCACGCGTGATTAA	PFHSYHNTIYAS*TMSTTRD*	-1.952	0.000913	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23232	NECADVPRRRKPLCCVFDIA			20	SLAY-screened peptide P1582			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGAGTGTGCTGATGTCCCCCGTCGGCGTAAGCCTTTGTGTTGTGTCTTCGATATCGCCTAA	NECADVPRRRKPLCCVFDIA*	-1.952	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23233	APRNCICYSRMCTSDFFTTI			20	SLAY-screened peptide P1583			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCTCGGAATTGTATCTGCTATAGTCGCATGTGTACCAGTGACTTCTTTACCACCATTTAA	APRNCICYSRMCTSDFFTTI*	-1.951	2.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23234	VRVYLCTKANFPMYVSIHPP			20	SLAY-screened peptide P1584			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGAGGGTGTACCTTTGCACGAAGGCCAATTTTCCTATGTATGTCAGCATCCATCCCCCCTAA	VRVYLCTKANFPMYVSIHPP*	-1.951	0.002189	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23235	LDTAVPYRIGDAVHAT			16	SLAY-screened peptide P1585			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGATACTGCTGTCCCTTACCGTATCGGCGACGCTGTTCACGCCACCTGATTTAAACTAACT	LDTAVPYRIGDAVHAT*FKLT	-1.951	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23236	APALLHPVQSDRTASHMNAW			20	SLAY-screened peptide P1586			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCCGCCCTTCTGCATCCGGTCCAGAGTGACCGTACTGCTTCGCACATGAATGCTTGGTAA	APALLHPVQSDRTASHMNAW*	-1.951	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23237	PHPP			4	SLAY-screened peptide P1587			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCATCCCCCCTAGAACGATTGGATTACGCACAATAGCTATATCATTGTGCCTCATGCGTAA	PHPP*NDWITHNSYIIVPHA*	-1.95	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23238	RLAMEQPLWPCMILMHNDVC			20	SLAY-screened peptide P1588			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCTTGCTATGGAGCAGCCGCTGTGGCCGTGCATGATCCTGATGCATAACGATGTTTGTTAA	RLAMEQPLWPCMILMHNDVC*	-1.95	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23239	LNTSFYLIGISISIGTSRSY			20	SLAY-screened peptide P1589			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAATACTAGTTTCTATCTTATCGGTATCTCTATCAGTATCGGGACGTCGCGTTCCTATTAA	LNTSFYLIGISISIGTSRSY*	-1.95	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23240	TWMNSFPLTYS			11	SLAY-screened peptide P1590			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTGGATGAACTCTTTTCCTCTCACTTATTCCTAGTGTTCCAATTCCCCTTACAGGGCCTAA	TWMNSFPLTYS*CSNSPYRA*	-1.949	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23241	QPDAEVPSPMPLCSNWRYLV			20	SLAY-screened peptide P1591			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCGGATGCGGAGGTTCCTTCCCCCATGCCCCTTTGCTCTAATTGGCGTTACCTCGTCTAA	QPDAEVPSPMPLCSNWRYLV*	-1.949	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23242	PSVSNWLCRYNTTVADKGL			19	SLAY-screened peptide P1592			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGTGTGAGTAACTGGCTTTGCCGGTATAACACCACCGTGGCGGACAAGGGTCTTTAGTAA	PSVSNWLCRYNTTVADKGL**	-1.949	0.000831	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23243	GTPMSCQIPCCGFGFRFEYL			20	SLAY-screened peptide P1593			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTACGCCGATGAGTTGCCAGATTCCCTGCTGTGGTTTCGGTTTCCGCTTCGAGTATCTGTAA	GTPMSCQIPCCGFGFRFEYL*	-1.948	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23244	ARFALHTCSHALAFASSS			18	SLAY-screened peptide P1594			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCGGTTCGCCCTTCACACTTGCTCTCATGCGCTGGCTTTCGCGTCTTCGTCTTAGACCTAA	ARFALHTCSHALAFASSS*T*	-1.948	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23245	VGGDSYHLQRRAHLCEPLQRL			21	SLAY-screened peptide P1595			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGGGGGGGACTCTTATCACCTGCAACGTCGTGCGCATTTATGCGAACCGCTCCAACGATTA	VGGDSYHLQRRAHLCEPLQRL	-1.948	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23246	PWVYRCVRPLNYPIASSTTCN			21	SLAY-screened peptide P1596			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGGGTTTATCGTTGTGTTCGTCCTCTCAATTATCCGATTGCTTCAAGTACAACGTGTAAC	PWVYRCVRPLNYPIASSTTCN	-1.947	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23247	EMIKFHDPFQASWAWTRKDE			20	SLAY-screened peptide P1597			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGATGATTAAGTTCCACGATCCGTTCCAGGCCTCTTGGGCTTGGACCCGCAAGGATGAGTAA	EMIKFHDPFQASWAWTRKDE*	-1.946	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23248	YYTLGTSITNGVSISTRSNY			20	SLAY-screened peptide P1598			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTACACGTTGGGTACCAGCATTACTAACGGGGTCAGTATTAGTACTCGTAGTAATTACTAA	YYTLGTSITNGVSISTRSNY*	-1.945	0.000471	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23249	ILLASIITPTTWIIGENSNF			20	SLAY-screened peptide P1599			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTTGCTGGCCAGCATCATCACGCCGACTACTTGGATCATTGGCGAGAATTCTAACTTCTAA	ILLASIITPTTWIIGENSNF*	-1.945	0.000107	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23250	HSIHIRVVSPWICIHLALSL			20	SLAY-screened peptide P1600			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTCGATCCATATTCGTGTGGTCTCGCCTTGGATTTGCATCCACCTTGCGCTCTCTCTTTAA	HSIHIRVVSPWICIHLALSL*	-1.944	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23251	IGNY			4	SLAY-screened peptide P1601			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGGTAACTATTAGTTCGTCTTTGCGTATCGGAATCATACTGTTTATTGTCGCAGGCACTAA	IGNY*FVFAYRNHTVYCRRH*	-1.943	0.000763	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23252	LELTGFEFFSDVTLD			15	SLAY-screened peptide P1602			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGAGCTTACTGGTTTCGAGTTTTTCTCTGACGTTACTCTCGATTAGCCGAATTAGTGCTAA	LELTGFEFFSDVTLD*PN*C*	-1.942	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23253	HIWLPIPSDDVS			12	SLAY-screened peptide P1603			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACATTTGGCTGCCGATCCCTAGCGATGATGTCAGCTAGAGGTTTCACCCTACTTCTATGTAA	HIWLPIPSDDVS*RFHPTSM*	-1.94	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23254	GRPVVACRVSIDPTSNRCHT			20	SLAY-screened peptide P1604			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCGCCCTGTGGTTGCGTGCCGTGTGTCTATTGATCCCACGTCGAATCGTTGTCATACCTAA	GRPVVACRVSIDPTSNRCHT*	-1.94	4.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23255	KTCSSQSTQVPCTKFKSYGA			20	SLAY-screened peptide P1605			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGACGTGTTCTTCTCAGAGTACGCAGGTCCCGTGCACCAAGTTCAAGTCTTACGGCGCCTAA	KTCSSQSTQVPCTKFKSYGA*	-1.94	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23256	PLCVTDLHKPYFGTSVNTIL			20	SLAY-screened peptide P1606			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTGTGTGTCACTGATCTCCACAAGCCGTATTTTGGTACCTCTGTTAACACTATTCTTTAA	PLCVTDLHKPYFGTSVNTIL*	-1.94	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23257	LIHHIYNASTFCIYTFSSID			20	SLAY-screened peptide P1607			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATTCATCATATCTATAACGCTTCGACGTTTTGTATCTATACGTTTAGTAGCATTGACTAA	LIHHIYNASTFCIYTFSSID*	-1.939	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23258	LYGHNRRSRDPWKNTKHGTA			20	SLAY-screened peptide P1608			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTATGGCCATAATAGGCGCAGCCGCGATCCGTGGAAGAATACTAAGCATGGTACGGCGTAA	LYGHNRRSRDPWKNTKHGTA*	-1.938	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23259	AA			2	SLAY-screened peptide P1609			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGCCTAGGGTTGCGATAACCGGGTTTACCCTGACGATACGTTCCCTTTTTAGTCCCCTTAA	AA*GCDNRVYPDDTFPF*SP*	-1.938	0.002494	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23260	SSWYFIVAHIYLITR			15	SLAY-screened peptide P1610			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAGTTGGTATTTCATTGTCGCTCATATTTACCTCATCACGAGGTAGTTTGTTATCCCCTAA	SSWYFIVAHIYLITR*FVIP*	-1.937	0.000568	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23261	FSVHSYNNYEPHVSTDSIAT			20	SLAY-screened peptide P1611			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTCCGTGCATTCTTACAATAACTACGAGCCTCACGTCTCTACTGACAGTATTGCTACCTAA	FSVHSYNNYEPHVSTDSIAT*	-1.937	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23262	PGPNPTIRAPPPDTPFVRFL			20	SLAY-screened peptide P1612			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGGCCCAACCCTACCATTCGCGCGCCTCCTCCGGATACGCCCTTCGTTCGGTTCCTCTAA	PGPNPTIRAPPPDTPFVRFL*	-1.937	0.000135	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23263	PDYIDVPTGSSSARKHQHML			20	SLAY-screened peptide P1613			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGATTATATTGACGTGCCTACTGGCTCGTCTTCTGCGCGTAAGCACCAGCACATGCTTTAA	PDYIDVPTGSSSARKHQHML*	-1.936	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23264	PFPLNVPFYSNWNVIAYNPQ			20	SLAY-screened peptide P1614			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTTTCCGTTGAACGTCCCTTTTTATAGCAATTGGAATGTCATTGCCTACAATCCCCAGTAA	PFPLNVPFYSNWNVIAYNPQ*	-1.936	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23265	PDTLPRVTPPSAHTSESRVS			20	SLAY-screened peptide P1615			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGACACGCTCCCGCGGGTGACGCCTCCTTCCGCCCACACTTCTGAGAGCCGGGTTTCCTAA	PDTLPRVTPPSAHTSESRVS*	-1.935	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23266	LLRKPMPVPPKKTDPYYKLT			20	SLAY-screened peptide P1616			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCTGCGCAAGCCTATGCCTGTTCCGCCTAAGAAGACTGATCCTTATTACAAGCTCACCTAA	LLRKPMPVPPKKTDPYYKLT*	-1.935	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23267	SIGIRARLVTNYRADLLDY			19	SLAY-screened peptide P1617			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCATTGGTATCCGCGCCAGGCTGGTTACTAATTACCGCGCCGACCTTTTGGATTATTAGTAA	SIGIRARLVTNYRADLLDY**	-1.935	0.00055	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23268	SVVFRYATSTLHPDMLFP			18	SLAY-screened peptide P1618			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGTGGTTTTTCGGTACGCCACGAGTACTCTTCACCCCGACATGCTGTTTCCTTAACTGAGT	SVVFRYATSTLHPDMLFP*LS	-1.935	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23269	CDDTDLSRFMLFVRHTKKSL			20	SLAY-screened peptide P1619			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGATGATACCGATCTTTCTCGCTTTATGTTGTTCGTTCGCCACACGAAGAAGAGTCTTTAA	CDDTDLSRFMLFVRHTKKSL*	-1.935	5.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23270	LSVNTPPSELFRVYRMDASM			20	SLAY-screened peptide P1620			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAGCGTCAACACTCCCCCGAGTGAGTTGTTTCGCGTTTATCGCATGGACGCTTCGATGTAA	LSVNTPPSELFRVYRMDASM*	-1.934	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23271	HTYTHVLTYTNSSSNPKTNA			20	SLAY-screened peptide P1621			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACCTACACCCATGTCCTTACGTATACGAATTCCTCCTCTAATCCGAAGACCAATGCCTAA	HTYTHVLTYTNSSSNPKTNA*	-1.934	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23272	CQLTMNVLNLLLRRFVKSAK			20	SLAY-screened peptide P1622			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCAGCTGACGATGAATGTGCTGAACCTCCTGCTCCGGCGGTTTGTCAAGAGCGCCAAGTAA	CQLTMNVLNLLLRRFVKSAK*	-1.933	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23273	ATIYAHYRS			9	SLAY-screened peptide P1623			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACCATCTATGCCCATTATAGGTCCTAGTTTCATTGTGGTAACTCCCATGGTTATAATTAA	ATIYAHYRS*FHCGNSHGYN*	-1.933	0.000446	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23274	PHTNADIVVRTPHSAPELGL			20	SLAY-screened peptide P1624			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCATACTAACGCGGACATTGTTGTGCGCACTCCGCACAGTGCGCCTGAGCTGGGCCTCTAA	PHTNADIVVRTPHSAPELGL*	-1.933	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23275	GNIKPMRPHVHRVDPCAYYH			20	SLAY-screened peptide P1625			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTAACATCAAGCCCATGAGGCCCCATGTGCATCGCGTCGACCCTTGCGCGTATTATCACTAA	GNIKPMRPHVHRVDPCAYYH*	-1.932	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23276	AGSSQTFAMKQASCSECH			18	SLAY-screened peptide P1626			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGGTTCTTCCCAGACCTTTGCCATGAAGCAGGCGAGTTGCTCTGAGTGTCATTAGAGCTAA	AGSSQTFAMKQASCSECH*S*	-1.932	0.004969	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23277	SMGSHDAPNYPRGTAFIILL			20	SLAY-screened peptide P1627			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCATGGGGAGTCACGACGCGCCTAACTATCCCAGGGGGACTGCTTTTATCATCCTCCTGTAA	SMGSHDAPNYPRGTAFIILL*	-1.932	0.0004	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23278	HLRYLERQHTSILHSSSPSD			20	SLAY-screened peptide P1628			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTTGCGTTACCTTGAGCGGCAGCATACGTCGATTCTCCACAGCTCGAGCCCCTCGGACTAA	HLRYLERQHTSILHSSSPSD*	-1.931	0.025526	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23279	SAYDDAWIMDNQIDLNRCDI			20	SLAY-screened peptide P1629			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGCCTATGATGATGCGTGGATCATGGACAATCAGATCGATCTTAATCGTTGTGATATCTAA	SAYDDAWIMDNQIDLNRCDI*	-1.931	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23280	PCLAAVGVRTDVFTNRVCTQ			20	SLAY-screened peptide P1630			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGCCTCGCGGCGGTTGGTGTGAGGACTGATGTTTTTACGAACCGCGTTTGCACCCAGTAA	PCLAAVGVRTDVFTNRVCTQ*	-1.931	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23281	PPLHLYIILLWPVILRMRLN			20	SLAY-screened peptide P1631			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCTCTGCACCTTTATATCATTCTGCTATGGCCGGTAATATTGCGGATGCGTCTTAACTGA	PPLHLYIILLWPVILRMRLN*	-1.93	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23282	CPTPCMRLLCLLVPRQSHLI			20	SLAY-screened peptide P1632			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCCACTCCCTGCATGCGCCTCCTCTGTCTGCTCGTCCCGCGCCAGTCCCATCTCATTTAA	CPTPCMRLLCLLVPRQSHLI*	-1.93	6.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23283	AQTHETSCQNSIINRF			16	SLAY-screened peptide P1633			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCAGACTCACGAGACCTCCTGCCAGAATTCTATCATTAACCGGTTCTAGCACGGGTAGTAA	AQTHETSCQNSIINRF*HG**	-1.929	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23284	DTGRPNRHIN			10	SLAY-screened peptide P1634			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACACGGGCCGCCCCAACCGGCATATCAATTAGATCCTCCTCTAGCTTGCCATTTACTAGTAA	DTGRPNRHIN*ILL*LAIY**	-1.929	5.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23285	TWVMIPTLLAHTPCTGAFNI			20	SLAY-screened peptide P1635			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTGGGTCATGATCCCCACGCTTCTTGCTCATACTCCTTGCACTGGTGCGTTTAACATTTAA	TWVMIPTLLAHTPCTGAFNI*	-1.929	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23286	GSARTAPNNSNNHQSCPVKF			20	SLAY-screened peptide P1636			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCAGCGCTCGTACGGCCCCGAATAACTCTAATAATCATCAGAGTTGTCCCGTTAAGTTCTAA	GSARTAPNNSNNHQSCPVKF*	-1.929	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23287	LPVAGLP			7	SLAY-screened peptide P1637			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCTGTGGCCGGTCTGCCCTAGCCCATCTACTCCTAGTCTTGTATTCCTTCTTAGTCGTAA	LPVAGLP*PIYS*SCIPS*S*	-1.928	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23288	SSFYLVTRMSFMSSHLYNKL			20	SLAY-screened peptide P1638			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTCTTTCTATCTCGTTACGCGTATGTCCTTCATGTCCAGCCATCTTTATAATAAGCTTTAA	SSFYLVTRMSFMSSHLYNKL*	-1.927	6.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23289	VHYCHHRVNYGHRVIR			16	SLAY-screened peptide P1639			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCATTATTGTCACCATCGCGTTAATTACGGCCATCGTGTCATTCGTTAGCCCGTTCCCTAA	VHYCHHRVNYGHRVIR*PVP*	-1.927	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23290	CHDGEMPPVQ			10	SLAY-screened peptide P1640			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCACGATGGTGAGATGCCGCCCGTTCAGTAGGTTAACGATGGCTGGCGTTAGATTATGTAA	CHDGEMPPVQ*VNDGWR*IM*	-1.927	0.002701	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23291	HQPSNRPF			8	SLAY-screened peptide P1641			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCAGCCGTCGAACCGTCCTTTTTAGCTTTGTAACCGTTACTAGTCGAACGTCAGCAACTAA	HQPSNRPF*LCNRY*SNVSN*	-1.927	0.000272	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23292	TVCREPVCYNYIRYHYFHLI			20	SLAY-screened peptide P1642			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGTTTGTCGTGAGCCCGTGTGTTATAATTACATCAGGTATCACTACTTTCACCTTATCTAA	TVCREPVCYNYIRYHYFHLI*	-1.927	3.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23293	GPPAASRPRLRDAPFDF			17	SLAY-screened peptide P1643			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCCCCCTGCCGCTTCTCGTCCCAGGCTTAGGGACGCTCCTTTCGACTTCTAGCGGCTTTAA	GPPAASRPRLRDAPFDF*RL*	-1.926	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23294	SVPYSPNTSTTACWLLPSIG			20	SLAY-screened peptide P1644			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGTGCCTTATTCCCCGAACACCAGTACCACCGCCTGCTGGTTGCTTCCTTCTATTGGTTAA	SVPYSPNTSTTACWLLPSIG*	-1.926	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23295	LIVKQSLSNYSTTFSTTGPSN			21	SLAY-screened peptide P1645			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGATTGTCAAGCAGTCGCTCTCTAACTATAGTACTACCTTCAGTACAACAGGTCCATCTAAC	LIVKQSLSNYSTTFSTTGPSN	-1.926	2.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23296	WHRYYSWLKRYWTYWKYGHF			20	SLAY-screened peptide P1646			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCACAGGTATTATAGCTGGCTCAAGCGTTATTGGACTTACTGGAAGTATGGTCATTTTTAA	WHRYYSWLKRYWTYWKYGHF*	-1.926	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23297	RLRSFRRYSCHR			12	SLAY-screened peptide P1647			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCTGAGGTCCTTTCGCCGCTATTCCTGCCACCGGTAGATGGTTCGGCTTGATATGTATTAA	RLRSFRRYSCHR*MVRLDMY*	-1.925	0.002233	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23298	AEIPCLMRYWHPRSLPVFGA			20	SLAY-screened peptide P1648			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGAGATCCCCTGCCTCATGCGGTACTGGCACCCGCGTTCGTTGCCGGTTTTCGGCGCTTAA	AEIPCLMRYWHPRSLPVFGA*	-1.925	5.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23299	RLSTAPRWSCLFCVMLNHTY			20	SLAY-screened peptide P1649			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTTAGTACGGCTCCGCGTTGGAGTTGTCTCTTCTGCGTCATGTTGAATCACACCTATTAA	RLSTAPRWSCLFCVMLNHTY*	-1.925	0.004099	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23300	PLSFTYLLAAPPSLYCGMYY			20	SLAY-screened peptide P1650			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTGTCTTTTACTTATCTCCTCGCTGCTCCTCCGTCTCTTTATTGCGGCATGTATTATTAA	PLSFTYLLAAPPSLYCGMYY*	-1.925	0.005837	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23301	APRTDHRSHNDLANAKMIAP			20	SLAY-screened peptide P1651			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCTCGCACTGATCACCGGAGTCACAACGACCTCGCGAACGCTAAGATGATCGCCCCCTAA	APRTDHRSHNDLANAKMIAP*	-1.925	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23302	ICFTMSIRCTPGFLCSTIGP			20	SLAY-screened peptide P1652			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTGCTTTACCATGTCCATTCGTTGCACTCCGGGTTTCCTGTGTTCTACGATTGGCCCTTAA	ICFTMSIRCTPGFLCSTIGP*	-1.923	0.000106	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23303	LFHSST			6	SLAY-screened peptide P1653			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTTTCATAGCTCCACGTAGGTTCGTAAGTATAGTGCTTTTTGGGCGTGCCTCAGTCACTAA	LFHSST*VRKYSAFWACLSH*	-1.923	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23304	TGIRLFLRLRAILRRIA			17	SLAY-screened peptide P1654			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACAGGAATTCGATTGTTTTTACGATTACGAGCAATATTACGCAGAATTGCATGAGTCATTAAC	TGIRLFLRLRAILRRIA*VIN	-1.922	0.015523	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23305	RRTPNGS			7	SLAY-screened peptide P1655			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGTACGCCGAACGGCTCCTAGGTCCGGGACCTTACGAGCAGCACCCAGCCGGCTGTGTAA	RRTPNGS*VRDLTSSTQPAV*	-1.922	0.000166	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23306	PLWPPSIPDIFHSITG			16	SLAY-screened peptide P1656			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCTGGCCTCCGTCCATTCCTGATATTTTTCACAGTATCACGGGCTAGCCGACGTGTTAA	PLWPPSIPDIFHSITG*PTC*	-1.921	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23307	SIGSVDFIAAMPHATPHWSG			20	SLAY-screened peptide P1657			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTATTGGTTCGGTGGATTTCATTGCGGCGATGCCTCATGCTACCCCCCATTGGTCCGGCTAA	SIGSVDFIAAMPHATPHWSG*	-1.921	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23308	INRYIIFAICYPRRKWARAD			20	SLAY-screened peptide P1658			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAACCGGTACATCATCTTCGCTATTTGTTATCCGCGCCGCAAGTGGGCTAGGGCTGATTAA	INRYIIFAICYPRRKWARAD*	-1.921	5.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23309	YSFAGHNPEYLALG			14	SLAY-screened peptide P1659			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTCGTTTGCTGGGCACAATCCCGAGTACCTCGCGCTTGGCTAGCTGTCCATTAAGGTCTAA	YSFAGHNPEYLALG*LSIKV*	-1.921	9.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23310	CNYVLDLVHAHMPNPARIRT			20	SLAY-screened peptide P1660			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAACTATGTTCTTGATCTTGTTCATGCCCATATGCCCAACCCCGCCCGTATCCGCACTTAA	CNYVLDLVHAHMPNPARIRT*	-1.92	0.006176	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23311	FYLFGFFLVLDLRNKILFSIN			21	SLAY-screened peptide P1661			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTACCTGTTCGGGTTTTTCCTTGTTCTCGACCTCCGTAACAAGATACTATTCTCCATTAAC	FYLFGFFLVLDLRNKILFSIN	-1.92	0.00118	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23312	PQLTRPEPHISAKGTAAAAP			20	SLAY-screened peptide P1662			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCAGCTCACGCGTCCGGAGCCCCACATTTCGGCTAAGGGCACTGCTGCTGCTGCGCCTTAA	PQLTRPEPHISAKGTAAAAP*	-1.918	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23313	IAIPSLTFDRIVTGGMAHNQ			20	SLAY-screened peptide P1663			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGCTATTCCGTCTCTCACTTTTGACCGTATTGTTACCGGTGGCATGGCGCACAATCAGTAA	IAIPSLTFDRIVTGGMAHNQ*	-1.918	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23314	RSAYIPITASYFRIAHMP			18	SLAY-screened peptide P1664			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCCGCCTACATCCCCATTACGGCTTCGTACTTTCGTATCGCCCATATGCCGTAGGGCTAA	RSAYIPITASYFRIAHMP*G*	-1.918	0.000227	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23315	SPYFHLYTPYLNRLLTRTPV			20	SLAY-screened peptide P1665			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCCCTACTTTCATCTGTATACTCCGTATCTCAACCGCCTGCTCACTAGGACCCCGGTCTAA	SPYFHLYTPYLNRLLTRTPV*	-1.916	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23316	YNLFHPNLQTIIMSIN			16	SLAY-screened peptide P1666			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACAACTTGTTTCACCCGAATCTCCAGACTATTATCATGTCTATTAATTAGTATTCTAACTAA	YNLFHPNLQTIIMSIN*YSN*	-1.915	0.003473	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23317	PPGIGDHTVCSLH			13	SLAY-screened peptide P1667			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCTGGTATCGGTGACCATACCGTGTGTTCCTTGCACTAGGCTCAGAACTTTGCCGTTTAA	PPGIGDHTVCSLH*AQNFAV*	-1.915	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23318	HSDVYCFTSSNHIILGVNYN			20	SLAY-screened peptide P1668			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAGCGACGTCTACTGCTTTACGAGTTCGAACCATATTATTCTGGGCGTTAACTATAATTAA	HSDVYCFTSSNHIILGVNYN*	-1.915	0.000717	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23319	ARLIYSPLSCQPPVLWFATN			20	SLAY-screened peptide P1669			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAGGCTCATCTATTCCCCCCTCTCTTGTCAGCCCCCCGTGCTTTGGTTTGCGACTAATTAA	ARLIYSPLSCQPPVLWFATN*	-1.915	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23320	RRSTVKPHHSQVHNPTYANA			20	SLAY-screened peptide P1670			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCGCTCCACTGTCAAGCCCCACCATTCGCAGGTCCATAACCCCACTTATGCGAATGCGTAA	RRSTVKPHHSQVHNPTYANA*	-1.914	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23321	PLYD			4	SLAY-screened peptide P1671			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTGTATGACTAGCGTGGTCACCATCCTTTTGGCCTTCTCAACCCGAATTCCCCCAGGTAA	PLYD*RGHHPFGLLNPNSPR*	-1.914	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23322	TVESTMDHRSAASTTNSRNT			20	SLAY-screened peptide P1672			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTCGAGAGCACCATGGACCACCGGTCCGCGGCTAGTACTACTAATTCTCGGAACACCTAA	TVESTMDHRSAASTTNSRNT*	-1.913	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23323	PTL			3	SLAY-screened peptide P1673			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACTCTCTAGCTCGTCTTCCCTACTAGTCTTAATTGCTAGCAGCTCAATATTATGATGTAA	PTL*LVFPTSLNC*QLNIMM*	-1.913	0.006062	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23324	DDHRYLSNPFIMCWTDCSIT			20	SLAY-screened peptide P1674			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGACCATCGCTACCTTTCTAATCCTTTTATTATGTGTTGGACTGACTGTTCTATTACGTAA	DDHRYLSNPFIMCWTDCSIT*	-1.913	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23325	CHYLMDNPYFIYV			13	SLAY-screened peptide P1675			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCACTATCTTATGGACAACCCCTATTTCATTTATGTCTAGCTCCTCAAGCTTGCGGGTAAC	CHYLMDNPYFIYV*LLKLAGN	-1.913	0.000159	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23326	SALIHYYWGGGYFLSGPLVP			20	SLAY-screened peptide P1676			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCGCTCATCCACTACTATTGGGGCGGTGGGTATTTTTTGTCCGGGCCTCTGGTCCCTTAA	SALIHYYWGGGYFLSGPLVP*	-1.913	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23327	GTFYGSSLARQSSCH			15	SLAY-screened peptide P1677			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTACTTTCTATGGGTCGTCGCTCGCCCGTCAGTCCTCGTGCCATTAGTACTCTCGTAAGTAA	GTFYGSSLARQSSCH*YSRK*	-1.912	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23328	AAADRINTDTSVHSSDMTNT			20	SLAY-screened peptide P1678			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCTGCGGATCGTATTAACACTGATACTTCCGTCCATAGTTCTGATATGACCAATACCTAA	AAADRINTDTSVHSSDMTNT*	-1.912	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23329	LN			2	SLAY-screened peptide P1679			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAACTAGCTTCCGTATGCTAGCGCCCTGCAGAATGGTTTTCGGGATGTCGTGCACTGTTAA	LN*LPYASALQNGFRDVVHC*	-1.912	7.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23330	FDLSIHIVIIINNWTLNFHF			20	SLAY-screened peptide P1680			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGACCTTTCGATCCACATTGTGATTATCATCAATAATTGGACCCTCAATTTCCACTTCTAA	FDLSIHIVIIINNWTLNFHF*	-1.912	0.000779	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23331	CTKQYFSLDTSFWNWPTYLL			20	SLAY-screened peptide P1681			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCACGAAGCAGTACTTTAGTCTCGATACTTCCTTTTGGAACTGGCCCACCTACCTCCTTTAA	CTKQYFSLDTSFWNWPTYLL*	-1.91	8.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23332	YHTDPSGMPPDSVFLIKHYC			20	SLAY-screened peptide P1682			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCATACTGATCCTAGTGGGATGCCCCCCGATTCGGTTTTTCTGATTAAGCATTATTGCTAA	YHTDPSGMPPDSVFLIKHYC*	-1.91	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23333	RYLAHPHSPVVFPPRRNSRT			20	SLAY-screened peptide P1683			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTATCTTGCGCACCCTCACTCTCCTGTTGTTTTCCCGCCTCGTAGGAATAGCCGTACCTAA	RYLAHPHSPVVFPPRRNSRT*	-1.91	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23334	PRWLSCKNNTNYYMTKNVAS			20	SLAY-screened peptide P1684			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGGTGGCTGTCGTGTAAGAACAATACCAATTATTACATGACCAAGAATGTTGCTAGTTAA	PRWLSCKNNTNYYMTKNVAS*	-1.91	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23335	DLVFTLVTVGGLTITILPVGN			21	SLAY-screened peptide P1685			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCTTGTGTTTACTCTCGTTACAGTTGGTGGCCTCACGATTACAATACTGCCTGTGGGTAAC	DLVFTLVTVGGLTITILPVGN	-1.91	0.00018	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23336	RVAPFTSDGDGFSNLSFCTRN			21	SLAY-screened peptide P1686			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGTGGCGCCCTTCACCAGCGATGGTGATGGTTTTAGTAATCTGAGTTTCTGTACACGTAAC	RVAPFTSDGDGFSNLSFCTRN	-1.909	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23337	PPSTNSAGSFWNGCFIAIGA			20	SLAY-screened peptide P1687			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCGTCTACCAATAGTGCGGGGTCGTTTTGGAATGGTTGTTTCATTGCGATCGGCGCTTAA	PPSTNSAGSFWNGCFIAIGA*	-1.909	0.000441	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23338	CLERIFVYLPPSTTV			15	SLAY-screened peptide P1688			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCTCGAGCGTATTTTCGTTTATCTGCCTCCGTCCACTACCGTGTAGATTCGTTCTCCGTAA	CLERIFVYLPPSTTV*IRSP*	-1.908	2.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23339	ALTTTLSRPHIYRSAAWSDN			20	SLAY-screened peptide P1689			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCTGACCACCACTCTTTCCCGTCCGCATATCTACCGCTCGGCTGCCTGGTCTGACAACTAA	ALTTTLSRPHIYRSAAWSDN*	-1.907	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23340	AGPVAPNPQPSWSPLNAILI			20	SLAY-screened peptide P1690			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGGCCCCGTTGCCCCTAATCCCCAGCCCTCCTGGTCGCCCCTGAATGCTATCCTGATTTAA	AGPVAPNPQPSWSPLNAILI*	-1.906	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23341	CSIYNRTVCFNLFLPITNPV			20	SLAY-screened peptide P1691			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAGCATTTACAATCGTACGGTTTGTTTTAACCTTTTTCTGCCTATTACCAACCCTGTGTAA	CSIYNRTVCFNLFLPITNPV*	-1.906	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23342	AAAVAAT			7	SLAY-screened peptide P1692			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCGGCCGTTGCCGCCACTTAGAACACCATTTTTCATCTGCATCGCTGCAACGCCAATTAA	AAAVAAT*NTIFHLHRCNAN*	-1.906	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23343	IQNVRPTSMPERRPSLDKDN			20	SLAY-screened peptide P1693			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCAGAACGTCCGCCCTACGTCCATGCCTGAGCGTAGGCCCTCGCTGGATAAGGATAATTAA	IQNVRPTSMPERRPSLDKDN*	-1.905	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23344	PLQDSDTPYSAVCSRLTAPR			20	SLAY-screened peptide P1694			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCCAGGATAGCGACACCCCTTATTCCGCCGTGTGTAGCCGCCTTACCGCGCCTCGTTAA	PLQDSDTPYSAVCSRLTAPR*	-1.905	0.006535	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23345	LRADATSDSRSQRPSRRTDF			20	SLAY-screened peptide P1695			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCGGGCCGATGCCACGTCTGACTCGCGTAGTCAGCGTCCTTCGCGTCGGACTGACTTCTAA	LRADATSDSRSQRPSRRTDF*	-1.904	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23346	HPFPGDWCPDSCNNQVIYSR			20	SLAY-screened peptide P1696			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCTTTCCCCGGTGATTGGTGCCCGGACTCCTGCAACAACCAGGTCATCTATTCTCGCTAA	HPFPGDWCPDSCNNQVIYSR*	-1.904	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23347	PTCFLAATFHFWMNLRPGGG			20	SLAY-screened peptide P1697			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACGTGTTTTTTGGCCGCCACCTTTCACTTCTGGATGAACCTCCGCCCGGGTGGCGGGTAA	PTCFLAATFHFWMNLRPGGG*	-1.904	0.001296	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23348	LLFYISPLYCEICDKDLEWS			20	SLAY-screened peptide P1698			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCTTTTTTACATTTCCCCCCTTTACTGTGAGATTTGCGATAAGGATCTCGAGTGGTCTTAA	LLFYISPLYCEICDKDLEWS*	-1.904	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23349	LRVYVPLRTDFVNCAHFSMY			20	SLAY-screened peptide P1699			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCGCGTGTACGTGCCGCTTAGGACCGACTTTGTCAATTGTGCTCATTTCTCGATGTACTAA	LRVYVPLRTDFVNCAHFSMY*	-1.903	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23350	VYFRPMRGCPPEGAGQNSFD			20	SLAY-screened peptide P1700			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTATTTCCGGCCCATGCGCGGGTGCCCCCCTGAGGGTGCCGGCCAGAACTCCTTCGACTAA	VYFRPMRGCPPEGAGQNSFD*	-1.903	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23351	PSRSFRSCPEAYYYWAQLYF			20	SLAY-screened peptide P1701			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCTCGTTCTTTCCGTTCCTGCCCTGAGGCTTACTATTACTGGGCTCAGCTGTATTTCTAA	PSRSFRSCPEAYYYWAQLYF*	-1.903	0.00029	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23352	IH			2	SLAY-screened peptide P1702			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCACTAGCTGGCGAAGTAGACGACCCGCCTTATCCGTTTGGCGCCTCATTCGAAGTACTAA	IH*LAK*TTRLIRLAPHSKY*	-1.903	0.012041	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23353	HDAVWQPPKPALTSNTIVRD			20	SLAY-screened peptide P1703			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGATGCGGTTTGGCAGCCGCCGAAGCCTGCCCTCACCAGTAATACGATTGTTCGTGATTAA	HDAVWQPPKPALTSNTIVRD*	-1.903	0.004043	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23354	YQRERIPLSCFTAVCKLTIM			20	SLAY-screened peptide P1704			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCAGCGCGAGCGCATCCCTCTTTCGTGTTTCACGGCTGTTTGCAAGCTTACGATCATGTAA	YQRERIPLSCFTAVCKLTIM*	-1.902	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23355	GSHKLLTTNKVLLSLAICPM			20	SLAY-screened peptide P1705			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCAGCCATAAGCTTCTGACTACTAACAAGGTCTTGCTGTCCCTCGCCATCTGTCCTATGTAA	GSHKLLTTNKVLLSLAICPM*	-1.902	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23356	DVATTRNNLSIFRYKCQSFP			20	SLAY-screened peptide P1706			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGTTGCCACTACCAGGAACAATCTTTCCATTTTCCGCTATAAGTGCCAGTCGTTTCCTTAA	DVATTRNNLSIFRYKCQSFP*	-1.901	0.000321	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23357	PLFTLGKRIINHLLRAYKYD			20	SLAY-screened peptide P1707			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTTTTACTCTGGGTAAGAGGATCATCAACCACTTGCTCAGGGCTTACAAGTATGACTAA	PLFTLGKRIINHLLRAYKYD*	-1.901	0.000232	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23358	SLWLRLSSSISLIVTALTLTN			21	SLAY-screened peptide P1708			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTTGTGGCTGCGACTCTCCTCGTCGATCTCCCTGATAGTGACTGCGCTTACTTTAACTAAC	SLWLRLSSSISLIVTALTLTN	-1.9	6.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23359	GYLSGAPCYYQL			12	SLAY-screened peptide P1709			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTACTTGTCGGGAGCCCCATGTTACTACCAACTCTGATAACTTTCTGACGTGTTAACTGAG	GYLSGAPCYYQL**LSDVLTE	-1.9	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23360	QFRVHQVYLRYFAPLYHSIS			20	SLAY-screened peptide P1710			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTTCCGTGTTCACCAGGTGTATCTGCGGTATTTCGCTCCCCTGTACCATTCTATTAGTTAA	QFRVHQVYLRYFAPLYHSIS*	-1.899	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23361	GWISNLPAVMMNPHNLPAWY			20	SLAY-screened peptide P1711			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTGGATTTCCAATCTTCCCGCTGTTATGATGAATCCTCATAATCTGCCCGCGTGGTACTAA	GWISNLPAVMMNPHNLPAWY*	-1.898	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23362	GQIKPIIMGCQWSGNHFLTS			20	SLAY-screened peptide P1712			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCAGATTAAGCCCATTATTATGGGTTGTCAGTGGAGTGGTAATCATTTCCTTACCTCTTAA	GQIKPIIMGCQWSGNHFLTS*	-1.898	0.003662	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23363	LATLSAACTSAP			12	SLAY-screened peptide P1713			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGGCCACCTTGAGTGCTGCCTGCACGTCCGCCCCTTAGCTGATTAACACCACTCACTTTTAA	LATLSAACTSAP*LINTTHF*	-1.898	0.006087	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23364	ACGQ			4	SLAY-screened peptide P1714			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTGCGGGCAGTAGAATTCTGCCACTTTTGGGCCCTCGCTTGACAAGCAGTTTTATACCTAA	ACGQ*NSATFGPSLDKQFYT*	-1.898	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23365	SLLNAP			6	SLAY-screened peptide P1715			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCTGCTTAACGCTCCGTAGTTGTTGCGCGACACTATTCACTACGACGCCGCGTCCGTTTAA	SLLNAP*LLRDTIHYDAASV*	-1.898	0.000206	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23366	IADFVPSLRVHFTPFWQTNA			20	SLAY-screened peptide P1716			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGCTGATTTCGTGCCCAGTCTGCGTGTGCACTTTACTCCCTTTTGGCAGACTAACGCCTAA	IADFVPSLRVHFTPFWQTNA*	-1.897	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23367	PGPLHATFRCPTIIIPTTTSN			21	SLAY-screened peptide P1717			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGTCCGCTCCATGCTACGTTCCGATGTCCAACCATTATTATCCCCACGACTACTTCTAAC	PGPLHATFRCPTIIIPTTTSN	-1.896	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23368	RNAS			4	SLAY-screened peptide P1718			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGAATGCGAGTTAGTCCCTCTATATTAATATTAGTCTCGCGCATCTCACTTACAACATTTAA	RNAS*SLYINISLAHLTYNI*	-1.896	0.000132	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23369	HLTEHGWTWRCVDIINKYNNY			21	SLAY-screened peptide P1719			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCTCACTGAGCACGGCTGGACCTGGCGCTGCGTTGACATTATTAATAAGTACAATAATTAC	HLTEHGWTWRCVDIINKYNNY	-1.896	0.00039	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23370	PPPVARHPCFKHVSVDSYRT			20	SLAY-screened peptide P1720			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTCCGGTTGCGCGCCATCCGTGTTTTAAGCACGTGTCTGTTGACTCTTATCGCACGTAA	PPPVARHPCFKHVSVDSYRT*	-1.896	0.029669	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23371	TNPNYRHNRSALEFTNIWFH			20	SLAY-screened peptide P1721			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAACCCCAATTATCGTCACAATAGGTCCGCGCTCGAGTTCACCAACATCTGGTTTCACTAA	TNPNYRHNRSALEFTNIWFH*	-1.895	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23372	HKKAGYSSGEWPRAFVFAPA			20	SLAY-screened peptide P1722			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAAGAAGGCGGGCTACTCTTCGGGTGAGTGGCCCAGGGCGTTCGTCTTCGCGCCTGCGTAA	HKKAGYSSGEWPRAFVFAPA*	-1.895	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23373	TTAFICILRRYRRDAYQIYLL			21	SLAY-screened peptide P1723			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGACCGCCTTTATTTGCATTCTTAGGCGCTATCGTCGCGATGCCTATCAGATCTACCTCCTA	TTAFICILRRYRRDAYQIYLL	-1.894	2.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23374	HYPPFGIFDMINCINSEITN			20	SLAY-screened peptide P1724			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTACCCCCCCTTCGGGATCTTCGACATGATCAATTGCATCAACTCCGAGATTACTAATTAA	HYPPFGIFDMINCINSEITN*	-1.894	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23375	NPGSIPAVYQSRHLTSPKCP			20	SLAY-screened peptide P1725			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCCCGGGTCTATCCCGGCTGTCTACCAGTCCAGGCACCTCACTTCCCCTAAGTGCCCGTAA	NPGSIPAVYQSRHLTSPKCP*	-1.893	0.000848	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23376	TTVTYLLSPFYYAGSIAGGF			20	SLAY-screened peptide P1726			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACCGTCACTTATCTCCTCTCGCCTTTCTACTATGCGGGTTCGATCGCCGGCGGGTTTTAA	TTVTYLLSPFYYAGSIAGGF*	-1.893	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23377	VLPGGPPWLHASTRYASEVS			20	SLAY-screened peptide P1727			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCTCCCTGGGGGCCCGCCTTGGCTGCATGCGTCTACTCGCTACGCTAGTGAGGTGTCCTAA	VLPGGPPWLHASTRYASEVS*	-1.893	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23378	TGTPAACYTSHYYRPAYNIS			20	SLAY-screened peptide P1728			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGGGACTCCTGCGGCCTGTTACACTAGTCATTATTACCGCCCCGCGTATAACATCAGTTAA	TGTPAACYTSHYYRPAYNIS*	-1.892	0.006083	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23379	HTSSRDCNIPVHLPYPHISL			20	SLAY-screened peptide P1729			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACGTCTAGCCGTGATTGTAACATTCCGGTCCATCTTCCCTACCCGCACATCTCTCTTTAA	HTSSRDCNIPVHLPYPHISL*	-1.892	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23380	DHGTKTGTYFPNCMNLTYML			20	SLAY-screened peptide P1730			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCACGGCACGAAGACTGGCACCTATTTTCCCAATTGTATGAATCTTACTTACATGCTTTAA	DHGTKTGTYFPNCMNLTYML*	-1.892	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23381	SWQTIQIFSL			10	SLAY-screened peptide P1731			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTGGCAGACCATCCAGATCTTTTCGTTGTAGCCTATCCTCCCCCCTCTCTGCCGGTGCTAA	SWQTIQIFSL*PILPPLCRC*	-1.891	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23382	RPAITCFHNRYLPSFTMEPL			20	SLAY-screened peptide P1732			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCGGCTATTACGTGTTTTCATAACAGGTACCTTCCTAGTTTTACGATGGAGCCTTTGTAA	RPAITCFHNRYLPSFTMEPL*	-1.891	0.000872	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23383	LSDVPYHVSAPKDPQCQNGG			20	SLAY-screened peptide P1733			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTCGGACGTGCCCTACCATGTCTCGGCGCCCAAGGACCCCCAGTGTCAGAACGGTGGGTAA	LSDVPYHVSAPKDPQCQNGG*	-1.891	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23384	PPSHRHDSNFYIALCISIICN			21	SLAY-screened peptide P1734			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCCTCGCACCGGCACGACTCTAACTTTTATATTGCCCTGTGCATCTCGATTATCTGTAAC	PPSHRHDSNFYIALCISIICN	-1.891	0.003733	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23385	CSTENKNYNNLRDIIPSQLI			20	SLAY-screened peptide P1735			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTCCACTGAGAACAAGAATTATAATAATCTGCGCGACATCATCCCTTCTCAGCTCATCTAA	CSTENKNYNNLRDIIPSQLI*	-1.891	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23386	RNKGHQTRYTANGGQYREHP			20	SLAY-screened peptide P1736			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGAACAAGGGCCATCAGACCCGGTACACCGCCAACGGTGGCCAGTATCGGGAGCATCCGTAA	RNKGHQTRYTANGGQYREHP*	-1.89	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23387	HLSNCSPDQIRP			12	SLAY-screened peptide P1737			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCTCAGCAATTGTAGTCCGGACCAGATTAGGCCTTAGATTCACGCGGCTGATAACTATTAA	HLSNCSPDQIRP*IHAADNY*	-1.89	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23388	PAHLNPFVTPYLIEASEPGS			20	SLAY-screened peptide P1738			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGCGCATTTGAATCCTTTCGTTACCCCCTATCTTATCGAGGCTAGTGAGCCTGGGAGCTAA	PAHLNPFVTPYLIEASEPGS*	-1.889	0.000755	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23389	TNRSAYPGTHYERSYLYYVI			20	SLAY-screened peptide P1739			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAATCGCAGCGCTTACCCGGGTACTCACTACGAGAGGTCCTACCTGTATTACGTGATCTAA	TNRSAYPGTHYERSYLYYVI*	-1.889	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23390	PLRGLAGTPLIHLYTIHSSY			20	SLAY-screened peptide P1740			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTGCGTGGCCTGGCTGGCACCCCGCTTATCCATCTTTATACCATTCACAGTTCTTATTAA	PLRGLAGTPLIHLYTIHSSY*	-1.889	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23391	PTYALICYSYRQATSPTATK			20	SLAY-screened peptide P1741			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACCTACGCGCTGATCTGTTACTCGTATCGCCAGGCCACCAGTCCGACTGCGACGAAGTAA	PTYALICYSYRQATSPTATK*	-1.888	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23392	KHDSYRPRGFLNTTFEIDQS			20	SLAY-screened peptide P1742			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCACGATTCCTACCGCCCGCGCGGCTTCCTCAATACTACGTTCGAGATTGATCAGAGCTAA	KHDSYRPRGFLNTTFEIDQS*	-1.888	0.012824	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23393	PSYCVPLLDRL			11	SLAY-screened peptide P1743			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGCTACTGTGTCCCGCTGCTCGACCGGTTGTAGCATAGTTCTTACCTGGACGTCCATTAA	PSYCVPLLDRL*HSSYLDVH*	-1.888	0.001088	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23394	IAVTFPLVTSTPHGHSRLSD			20	SLAY-screened peptide P1744			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGCGGTTACGTTTCCTCTTGTGACGAGTACCCCTCACGGTCATTCTAGGCTGAGTGATTAA	IAVTFPLVTSTPHGHSRLSD*	-1.888	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23395	CGTSVRPIIPHKHCYDVTTP			20	SLAY-screened peptide P1745			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGGCACTTCGGTCCGTCCTATTATTCCTCATAAGCATTGCTACGATGTGACCACTCCTTAA	CGTSVRPIIPHKHCYDVTTP*	-1.887	5.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23396	IGSGNADTYLHGSLNQHLHC			20	SLAY-screened peptide P1746			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGGGTCGGGTAACGCTGATACGTATCTTCACGGTTCTCTTAATCAGCACCTTCATTGCTAA	IGSGNADTYLHGSLNQHLHC*	-1.887	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23397	SHTPPLDNSISFDSDTTYYF			20	SLAY-screened peptide P1747			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCACACCCCGCCGCTGGACAACAGCATCTCGTTTGATAGCGATACGACGTACTACTTCTAA	SHTPPLDNSISFDSDTTYYF*	-1.887	2.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23398	PSILSYGSYCHPTWHDFGSP			20	SLAY-screened peptide P1748			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGCATCCTGTCCTATGGTAGTTATTGTCACCCTACTTGGCATGATTTCGGTTCTCCCTAA	PSILSYGSYCHPTWHDFGSP*	-1.886	0.021084	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23399	PTFESNLKETLSNTATRSSV			20	SLAY-screened peptide P1749			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACTTTTGAGAGCAACCTTAAGGAGACTTTGTCTAACACTGCTACGCGTAGTTCGGTCTAA	PTFESNLKETLSNTATRSSV*	-1.885	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23400	SGFPYISPPYISNCNFNHDT			20	SLAY-screened peptide P1750			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGGCTTCCCGTATATTTCTCCGCCCTACATCTCGAACTGCAACTTCAACCACGACACGTAA	SGFPYISPPYISNCNFNHDT*	-1.885	0.005445	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23401	NRKPRRFTCYLNAVLHTSPP			20	SLAY-screened peptide P1751			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCGTAAGCCTCGCCGTTTCACCTGTTACCTGAACGCTGTTCTTCATACCTCTCCGCCGTAA	NRKPRRFTCYLNAVLHTSPP*	-1.885	0.000213	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23402	HGPGARSFRYHDHNRRCDGR			20	SLAY-screened peptide P1752			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGGCCCTGGTGCCCGTAGTTTTCGTTATCACGATCACAACCGTCGTTGTGACGGTAGGTAA	HGPGARSFRYHDHNRRCDGR*	-1.885	0.000132	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23403	HRCYHSSPSFYDFFIWRFPW			20	SLAY-screened peptide P1753			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCGCTGTTATCACTCTAGCCCGAGTTTTTACGATTTTTTTATTTGGCGTTTCCCTTGGTAA	HRCYHSSPSFYDFFIWRFPW*	-1.884	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23404	FHRPQDQSHSYTPTVATCTQ			20	SLAY-screened peptide P1754			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCACAGGCCGCAGGATCAGTCTCATTCGTATACTCCCACCGTTGCGACGTGCACGCAGTAA	FHRPQDQSHSYTPTVATCTQ*	-1.884	0.000278	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23405	PHSNSQPDSRHYPHCWYALL			20	SLAY-screened peptide P1755			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCACAGTAATTCGCAGCCCGATTCCCGGCACTACCCCCACTGTTGGTATGCGCTTCTTTAA	PHSNSQPDSRHYPHCWYALL*	-1.883	0.006844	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23406	FTYQATHPAPELREASTIGS			20	SLAY-screened peptide P1756			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCACGTACCAGGCCACTCATCCCGCGCCGGAGCTTCGGGAGGCTAGTACGATTGGTAGTTAA	FTYQATHPAPELREASTIGS*	-1.883	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23407	SNYVRYK			7	SLAY-screened peptide P1757			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCAACTATGTCAGGTATAAGTAGCCCTTGCGTGTTAATCATGAGAGCCGGACCATCTTCTAA	SNYVRYK*PLRVNHESRTIF*	-1.883	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23408	SQRLAHYKHDNFNVYKHHYS			20	SLAY-screened peptide P1758			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCAGCGCCTTGCGCATTACAAGCATGACAATTTCAATGTGTATAAGCACCATTATTCTTAA	SQRLAHYKHDNFNVYKHHYS*	-1.883	0.002852	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23409	ARSETCFRPRPGSSSRLGYV			20	SLAY-screened peptide P1759			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCGTTCCGAGACCTGCTTTCGGCCTCGTCCCGGTAGCTCTTCTCGTCTCGGTTATGTCTAA	ARSETCFRPRPGSSSRLGYV*	-1.881	8.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23410	LPGCYTHCHDHHQPLRRPALN			21	SLAY-screened peptide P1760			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCGGGCTGCTATACGCACTGTCATGATCACCATCAGCCGTTACGAAGACCAGCCCTTAAC	LPGCYTHCHDHHQPLRRPALN	-1.881	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23411	HCMLLLA			7	SLAY-screened peptide P1761			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGTATGTTGCTTCTTGCGTAGGTGACGACCGCTATGTGGCGTGTTCACTGCCTGATTTAA	HCMLLLA*VTTAMWRVHCLI*	-1.881	0.002946	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23412	TRTRTLIILLRIFSRLIRSSN			21	SLAY-screened peptide P1762			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGAGAACCAGAACCCTTATTATATTATTGAGAATTTTTTCGAGACTAATACGCAGCAGTAAC	TRTRTLIILLRIFSRLIRSSN	-1.881	8.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23413	WILS			4	SLAY-screened peptide P1763			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGATTCTCTCTTAGTACTTCTCCAACCCCACTGACAGGTTTCCGCTGTAGGACCATCCTTAA	WILS*YFSNPTDRFPL*DHP*	-1.881	0.01302	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23414	HTILSSIAADDMVIRANTPVN			21	SLAY-screened peptide P1764			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACTATCCTCTCTAGTATCGCCGCGGATGATATGGTTATTAGGGCTAATACGCCGGTTAAC	HTILSSIAADDMVIRANTPVN	-1.88	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23415	HVRPLTQNSIDNNLSLSRAH			20	SLAY-screened peptide P1765			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGTGCGTCCTCTTACCCAGAATTCGATTGACAATAACCTTTCTCTCTCCCGCGCGCATTAA	HVRPLTQNSIDNNLSLSRAH*	-1.88	0.000249	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23416	IN			2	SLAY-screened peptide P1766			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAACTAGCTTTCTACTAATACTTAGGGCCCTTTTGATAACGTTGTGCTTCCCACTCCCTAA	IN*LSTNT*GPFDNVVLPTP*	-1.88	0.000183	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23417	LMISNRHG			8	SLAY-screened peptide P1767			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCATGATCTCGAATCGGCATGGTTAGTACAATTCCCGCAACGATCTCCTCGCCTGCAGCTAA	LMISNRHG*YNSRNDLLACS*	-1.879	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23418	MTCTG			5	SLAY-screened peptide P1768			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGACTTGCACCGGCTAGCCGGGTCTCTCCCCCCCTCCGCTCGGTTCTATTTTTGACCCGTAA	MTCTG*PGLSPPPLGSIFDP*	-1.878	5.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23419	DLYAS			5	SLAY-screened peptide P1769			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCTTTATGCCAGCTAGTTTCATAGCACTGACCCCCCTTACATTGATGTGTTGGTTATTTAA	DLYAS*FHSTDPPYIDVLVI*	-1.878	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23420	SVRHQHLLLCVRGTCLLKFK			20	SLAY-screened peptide P1770			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGTCCGGCATCAGCATCTGCTTTTGTGTGTTCGGGGGACGTGCCTCCTTAAGTTTAAGTAA	SVRHQHLLLCVRGTCLLKFK*	-1.877	1.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23421	SSTVVILYPLVQIFRRYGRY			20	SLAY-screened peptide P1771			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTCTACTGTGGTGATCCTTTATCCCCTGGTCCAGATCTTTCGTCGCTACGGCCGCTACTAA	SSTVVILYPLVQIFRRYGRY*	-1.877	0.006572	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23422	MPVTFLGINTCAYPPRRDYK			20	SLAY-screened peptide P1772			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCCCGTTACTTTTCTGGGTATCAATACTTGCGCGTATCCCCCTAGGAGGGACTACAAGTAA	MPVTFLGINTCAYPPRRDYK*	-1.876	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23423	TVCIPSASSTQFASMRPPWI			20	SLAY-screened peptide P1773			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGTGTGCATCCCCTCTGCGAGCAGCACCCAGTTTGCGAGCATGCGCCCTCCCTGGATTTAA	TVCIPSASSTQFASMRPPWI*	-1.876	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23424	DTPTVIISNPHRHTSFGAGY			20	SLAY-screened peptide P1774			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACACGCCGACCGTCATTATTTCTAACCCTCACAGGCATACCTCCTTTGGCGCTGGCTACTAA	DTPTVIISNPHRHTSFGAGY*	-1.875	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23425	PRIPIRSRRSNSTTI			15	SLAY-screened peptide P1775			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGCATTCCGATTCGGTCTCGTCGCTCTAATAGCACTACGATTTAGTGCAGTTTCGATTAA	PRIPIRSRRSNSTTI*CSFD*	-1.874	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23426	LRFKPLHLDFAFHSSLPMVS			20	SLAY-screened peptide P1776			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCGTTTTAAGCCTCTTCATCTTGATTTCGCCTTCCATTCCTCCTTGCCGATGGTGAGCTAA	LRFKPLHLDFAFHSSLPMVS*	-1.874	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23427	YNSTAKLDLCGVTNHTRIRW			20	SLAY-screened peptide P1777			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAACTCGACTGCGAAGCTTGACCTTTGTGGCGTCACCAACCATACTCGCATCAGGTGGTAA	YNSTAKLDLCGVTNHTRIRW*	-1.874	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23428	YKRIHIAISFHGWSVHFELH			20	SLAY-screened peptide P1778			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAAGCGCATTCATATCGCGATTAGTTTTCATGGTTGGTCCGTTCACTTTGAGCTTCACTAA	YKRIHIAISFHGWSVHFELH*	-1.874	0.014519	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23429	DWPHFRIGLFISPQVYTHLS			20	SLAY-screened peptide P1779			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTGGCCTCACTTTCGCATTGGTCTTTTTATCAGCCCGCAGGTTTACACTCATCTTTCTTAA	DWPHFRIGLFISPQVYTHLS*	-1.872	0.016207	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23430	PKGLGMSLARSIDYWCGFLI			20	SLAY-screened peptide P1780			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAAGGGCCTCGGCATGAGTCTCGCTCGGAGTATCGACTACTGGTGTGGTTTTCTGATCTAA	PKGLGMSLARSIDYWCGFLI*	-1.871	9.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23431	DAVPTAHSSRKYLPQDSPTT			20	SLAY-screened peptide P1781			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGCTGTCCCTACTGCCCACAGCAGTAGGAAGTACTTGCCCCAGGACTCCCCGACCACTTAA	DAVPTAHSSRKYLPQDSPTT*	-1.87	8.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23432	FWLYLSCICILNHSHN			16	SLAY-screened peptide P1782			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTGGCTCTACCTGAGCTGCATTTGCATCCTTAATCACAGTCATAATTAGCACTGTAACTAA	FWLYLSCICILNHSHN*HCN*	-1.87	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23433	LHADAIHIYELGYHPLLYVS			20	SLAY-screened peptide P1783			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCACGCCGATGCTATTCATATCTACGAGCTTGGTTATCACCCGCTGCTCTACGTTTCCTAA	LHADAIHIYELGYHPLLYVS*	-1.87	0.001007	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23434	GTPKPPFRSNVGSYETALYH			20	SLAY-screened peptide P1784			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTACGCCGAAGCCCCCGTTCCGTTCTAACGTGGGTTCGTATGAGACCGCGCTCTACCATTAA	GTPKPPFRSNVGSYETALYH*	-1.87	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23435	GAPSVNCTNR			10	SLAY-screened peptide P1785			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGCTCCCTCGGTTAACTGCACGAATCGTTAGACGAACGGCCGCGGTAATTGCATCTCTTAA	GAPSVNCTNR*TNGRGNCIS*	-1.87	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23436	HTHFNCSVLARVRTVPTVAIN			21	SLAY-screened peptide P1786			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACGCATTTCAATTGTTCCGTCTTGGCTAGGGTTAGGACTGTTCCTACAGTCGCTATTAAC	HTHFNCSVLARVRTVPTVAIN	-1.87	4.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23437	FLSHI			5	SLAY-screened peptide P1787			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCTCTCCCACATTTAGCTCCGTAATATGTTGCGTTTTGATCAGATTGCTTGGCTTCCTTAA	FLSHI*LRNMLRFDQIAWLP*	-1.87	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23438	RPIPMYFCRHYNLAWTQATF			20	SLAY-screened peptide P1788			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCCATTCCTATGTACTTTTGTCGCCACTACAATCTTGCGTGGACTCAGGCGACGTTTTAA	RPIPMYFCRHYNLAWTQATF*	-1.869	0.006711	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23439	IRNGSANSVMSTYYPPSLIT			20	SLAY-screened peptide P1789			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAGGAACGGGTCCGCTAATTCCGTTATGAGCACTTACTATCCGCCTTCCCTCATTACCTAA	IRNGSANSVMSTYYPPSLIT*	-1.868	5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23440	AQVLVTMVSTALAP			14	SLAY-screened peptide P1790			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCAGGTCTTGGTCACTATGGTGAGCACTGCCCTTGCTCCTTAGGGGGCTAGCCACCCCTAA	AQVLVTMVSTALAP*GASHP*	-1.867	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23441	SSPVLYTSFTIGLWTYRHCE			20	SLAY-screened peptide P1791			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGAGTCCCGTTTTGTATACTAGCTTTACTATTGGGCTGTGGACTTACCGCCATTGTGAGTAA	SSPVLYTSFTIGLWTYRHCE*	-1.865	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23442	NTCISPVTNNYTLAHVFLHN			20	SLAY-screened peptide P1792			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACACGTGTATTAGTCCCGTTACTAATAACTATACCCTTGCCCACGTTTTCTTGCATAATTAA	NTCISPVTNNYTLAHVFLHN*	-1.865	0.005656	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23443	KHWNTECCCSGNGN			14	SLAY-screened peptide P1793			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCATTGGAATACCGAGTGCTGTTGTAGTGGCAACGGTAATTAGTCGCTTCACATGCTTTAC	KHWNTECCCSGNGN*SLHMLY	-1.865	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23444	RCRRLIAFYKYWRMRSIWLRN			21	SLAY-screened peptide P1794			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGCCGGCGCCTTATCGCGTTTTACAAGTACTGGCGCATGCGTTCAATTTGGCTGCGTAAC	RCRRLIAFYKYWRMRSIWLRN	-1.865	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23445	PGNCRPAGFNRIRDSPSYAF			20	SLAY-screened peptide P1795			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGCAACTGTCGGCCTGCGGGCTTTAATCGTATTCGGGATTCCCCGAGTTATGCGTTCTAA	PGNCRPAGFNRIRDSPSYAF*	-1.865	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23446	HPTIP			5	SLAY-screened peptide P1796			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCGACTATCCCCTAGTACTACAATGATACGCATCGTCTTATTGCTTCGACTCACTATTAA	HPTIP*YYNDTHRLIASTHY*	-1.865	6.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23447	FPTAPHGGDLYRDLPALSCT			20	SLAY-screened peptide P1797			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCCTACTGCTCCTCACGGTGGTGACCTCTACCGTGATCTGCCTGCGCTTAGTTGCACCTAA	FPTAPHGGDLYRDLPALSCT*	-1.865	0.000171	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23448	LNHMHNLLHLLTRLARSFRRN			21	SLAY-screened peptide P1798			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAATCATATGCACAACCTCCTTCATTTGCTAACACGGCTAGCAAGGTCATTCCGTCGTAAC	LNHMHNLLHLLTRLARSFRRN	-1.864	0.000163	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23449	SSTVFAKPGRLIPSFSFFGC			20	SLAY-screened peptide P1799			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAGCACCGTTTTTGCGAAGCCTGGGCGTTTGATCCCCAGCTTCTCCTTTTTCGGTTGCTAA	SSTVFAKPGRLIPSFSFFGC*	-1.864	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23450	TCS			3	SLAY-screened peptide P1800			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTGCTCTTAGCTGCCCGGGCTGCACACTAACCCTATTTTTACCATGCGGTGTCGGTAGTAA	TCS*LPGLHTNPIFTMRCR**	-1.864	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23451	PMCVKRLLTLLTRRFRLAVSN			21	SLAY-screened peptide P1801			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATGTGTGTGAAGCGTCTGTTGACTCTGTTGACCCGTCGTTTTAGGCTCGCTGTGAGTAAC	PMCVKRLLTLLTRRFRLAVSN	-1.863	0.000212	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23452	PPLFQPSQAVQYKGSPDSGS			20	SLAY-screened peptide P1802			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTCTGTTTCAGCCTTCCCAGGCCGTTCAGTACAAGGGGTCCCCTGATTCGGGCAGCTAA	PPLFQPSQAVQYKGSPDSGS*	-1.863	0.007613	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23453	APVLNCYMLSIFRNVR			16	SLAY-screened peptide P1803			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCGGTTTTGAATTGCTATATGCTGAGTATTTTTCGGAACGTCCGTTAGACCACTATTTAA	APVLNCYMLSIFRNVR*TTI*	-1.862	0.010706	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23454	ALFMQYATHRRQLSPGRFVH			20	SLAY-screened peptide P1804			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCTGTTTATGCAGTACGCCACCCATCGGCGTCAGCTTAGTCCGGGGCGTTTCGTCCACTAA	ALFMQYATHRRQLSPGRFVH*	-1.862	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23455	AAPLLHPWGTPLYDRVLITY			20	SLAY-screened peptide P1805			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGCCCCTCTTCTGCATCCTTGGGGTACTCCGCTTTACGATAGGGTTTTGATCACTTACTAA	AAPLLHPWGTPLYDRVLITY*	-1.862	0.001199	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23456	SINPISDQLEVHPNCHMVHH			20	SLAY-screened peptide P1806			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTATTAATCCTATCAGTGACCAGCTTGAGGTCCATCCGAATTGCCATATGGTCCACCATTAA	SINPISDQLEVHPNCHMVHH*	-1.862	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23457	RQPSIGLSHDYNPPFHRSSQ			20	SLAY-screened peptide P1807			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCAGCCCTCTATTGGCCTGTCTCATGACTATAACCCCCCTTTCCATAGGAGTTCTCAGTAA	RQPSIGLSHDYNPPFHRSSQ*	-1.861	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23458	RYTNYSTCNLE			11	SLAY-screened peptide P1808			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTATACTAATTATTCTACTTGTAACCTTGAGTAGCGTAGCATCATTATTGCGGGGATCTAA	RYTNYSTCNLE*RSIIIAGI*	-1.861	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23459	HSTQLNFSSVNTINILNLSN			20	SLAY-screened peptide P1809			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTCCACTCAGCTTAACTTTAGTTCTGTGAACACCATCAACATTCTTAACCTCAGTAACTAA	HSTQLNFSSVNTINILNLSN*	-1.86	2.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23460	LNNCKPPVLPTTSVHPFSWR			20	SLAY-screened peptide P1810			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAACAATTGCAAGCCTCCTGTCCTCCCTACTACTTCGGTGCATCCTTTTTCGTGGCGGTAA	LNNCKPPVLPTTSVHPFSWR*	-1.859	0.024152	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23461	RYIEGSHRYFMHPVYYLFEP			20	SLAY-screened peptide P1811			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTATATCGAGGGCTCGCATCGGTATTTTATGCACCCCGTGTATTACCTCTTCGAGCCCTAA	RYIEGSHRYFMHPVYYLFEP*	-1.859	0.005173	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23462	HYPRIDHNFRDF			12	SLAY-screened peptide P1812			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTACCCCCGGATCGACCATAACTTTCGTGACTTTTAGTATCATTCTAGCTGCTGTTCGTAA	HYPRIDHNFRDF*YHSSCCS*	-1.858	0.000267	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23463	YNGLNSPSYTEGCVYLV			17	SLAY-screened peptide P1813			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAATGGCCTCAACAGTCCCTCTTATACGGAGGGTTGCGTCTACTTGGTTTAGGTGTCCTAC	YNGLNSPSYTEGCVYLV*VSY	-1.858	0.000815	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23464	CRRVNAYRPIKSSGSQNSLY			20	SLAY-screened peptide P1814			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAGGCGCGTTAATGCCTACCGCCCTATTAAGTCGTCCGGTTCTCAGAACAGCCTCTACTAA	CRRVNAYRPIKSSGSQNSLY*	-1.858	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23465	NPWLSHFVRMLILRLFRG			18	SLAY-screened peptide P1815			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCCCTGGCTTTCGCATTTCGTTAGGATGTTAATATTAAGGCTATTCAGGGGCTGAAGTAAC	NPWLSHFVRMLILRLFRG*SN	-1.858	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23466	SWADIRRGQNTLQKTDD			17	SLAY-screened peptide P1816			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTGGGCTGACATCCGTCGGGGCCAGAACACTTTGCAGAAGACTGACGACTAGGCTACTTAA	SWADIRRGQNTLQKTDD*AT*	-1.856	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23467	VCKRRYPPDSSPPRSDQAST			20	SLAY-screened peptide P1817			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTGCAAGAGGAGGTACCCGCCTGATTCCAGCCCGCCTCGGAGTGATCAGGCTAGTACCTAA	VCKRRYPPDSSPPRSDQAST*	-1.856	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23468	NEPKTTSVLTLFCLVCLLPSN			21	SLAY-screened peptide P1818			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGAGCCGAAGACGACCAGCGTATTAACACTATTTTGTCTAGTATGTCTACTCCCTAGTAAC	NEPKTTSVLTLFCLVCLLPSN	-1.855	0.007009	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23469	CLFPCNTHCYYFLRVLPIDT			20	SLAY-screened peptide P1819			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTCTTTCCTTGCAACACCCATTGTTATTATTTTTTGCGGGTGCTTCCCATTGACACGTAA	CLFPCNTHCYYFLRVLPIDT*	-1.855	0.001135	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23470	HDVAHLLRCGSNPGAWAQTS			20	SLAY-screened peptide P1820			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGATGTCGCCCATCTGCTTCGCTGCGGTAGTAACCCTGGCGCGTGGGCCCAGACTTCTTAA	HDVAHLLRCGSNPGAWAQTS*	-1.855	0.000156	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23471	GRHIIFLSVVMLRFSRRDSV			20	SLAY-screened peptide P1821			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCGTCATATTATTTTTTTGAGCGTCGTGATGTTGAGGTTCAGCAGGCGGGATAGTGTCTAA	GRHIIFLSVVMLRFSRRDSV*	-1.855	0.005455	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23472	SANYVVHPAINGGTPPWSLI			20	SLAY-screened peptide P1822			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGCTAATTACGTGGTCCACCCGGCCATCAACGGGGGGACGCCGCCCTGGTCGCTTATCTAA	SANYVVHPAINGGTPPWSLI*	-1.855	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23473	NSYAYPYRFYLLSLTSANRA			20	SLAY-screened peptide P1823			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTCCTATGCCTATCCCTATCGTTTCTACCTCCTTTCCCTCACGAGTGCCAATCGTGCCTAA	NSYAYPYRFYLLSLTSANRA*	-1.854	0.008738	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23474	TSLVAAAVDARRARMHESLP			20	SLAY-screened peptide P1824			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAGCCTGGTTGCTGCTGCTGTTGACGCTAGGCGGGCCAGGATGCACGAGTCCCTGCCTTAA	TSLVAAAVDARRARMHESLP*	-1.854	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23475	PVAHVPKLDLNCFLAPNCYS			20	SLAY-screened peptide P1825			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGTTGCTCATGTTCCGAAGCTCGATCTCAACTGCTTTTTGGCCCCTAATTGTTACAGTTAA	PVAHVPKLDLNCFLAPNCYS*	-1.853	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23476	NPRKLATSKNCHIPQILDF			19	SLAY-screened peptide P1826			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCCGCGCAAGCTTGCGACGTCCAAGAATTGCCACATCCCGCAGATCCTCGATTTTTAGTAA	NPRKLATSKNCHIPQILDF**	-1.853	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23477	NLHPDGKTLTHVLSRALHPP			20	SLAY-screened peptide P1827			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCTCCACCCTGACGGCAAGACCTTGACCCATGTGCTCTCCAGGGCGTTGCATCCTCCGTAA	NLHPDGKTLTHVLSRALHPP*	-1.853	0.006547	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23478	ADENLSARGVFRCLHY			16	SLAY-screened peptide P1828			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGATGAGAACCTGTCTGCTCGAGGCGTCTTTCGCTGCCTACATTACTAGTTACTAACTGAG	ADENLSARGVFRCLHY*LLTE	-1.852	9.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23479	PA			2	SLAY-screened peptide P1829			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGCGTAGTCGGTCATCCATGCCACTGAGGCGCACGTGCAGAAGTTTTTTGATCTTAACTAA	PA*SVIHATEAHVQKFFDLN*	-1.852	0.000133	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23480	RNTNVHMLEPWLTDSLILLGN			21	SLAY-screened peptide P1830			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAATACCAATGTGCATATGTTGGAGCCTTGGCTTACCGATAGCCTCATACTACTGGGTAAC	RNTNVHMLEPWLTDSLILLGN	-1.852	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23481	CSSPFSPQVF			10	SLAY-screened peptide P1831			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTCGAGTCCCTTTTCGCCGCAGGTTTTTTAGCCCTATGGCCTTTAGGGGATTACGTGCTAA	CSSPFSPQVF*PYGL*GITC*	-1.85	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23482	HRPTPQPRCNVIHSRQNNDL			20	SLAY-screened peptide P1832			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCGTCCGACGCCTCAGCCTCGTTGTAACGTTATCCATTCCAGGCAGAATAACGACCTCTAA	HRPTPQPRCNVIHSRQNNDL*	-1.85	0.043603	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23483	SMPTSYPNSYDVA			13	SLAY-screened peptide P1833			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTATGCCTACTAGCTATCCTAACTCTTATGATGTCGCCTAGATTTCGGTGGCGAAGCGGTAA	SMPTSYPNSYDVA*ISVAKR*	-1.85	0.000227	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23484	GPAYLYISYWTLNYNPYIYK			20	SLAY-screened peptide P1834			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCCGGCTTACCTGTACATTAGCTATTGGACCTTGAATTACAACCCCTATATTTATAAGTAA	GPAYLYISYWTLNYNPYIYK*	-1.849	0.013233	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23485	PPPGGTPSNPYEAGVTRPHF			20	SLAY-screened peptide P1835			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCGCCTGGCGGGACCCCGTCGAATCCTTACGAGGCCGGCGTGACCAGGCCCCATTTCTAA	PPPGGTPSNPYEAGVTRPHF*	-1.849	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23486	THYRWLLFPQF			11	SLAY-screened peptide P1836			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCATTATCGTTGGCTCTTGTTCCCACAATTCTAGCTCGTGTTATTGTATCTAACTGAGTAA	THYRWLLFPQF*LVLLYLTE*	-1.848	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23487	LAPHLYATRCVLSVLLIQHN			20	SLAY-screened peptide P1837			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGCTCCGCATCTTTATGCGACCCGTTGCGTTCTTTCCGTGCTGTTGATTCAGCATAACTAA	LAPHLYATRCVLSVLLIQHN*	-1.848	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23488	TNYGRTFIGADIFI			14	SLAY-screened peptide P1838			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAATTATGGTCGCACCTTTATCGGGGCGGATATCTTCATCTGAGTAACGATGGCGGTTAAC	TNYGRTFIGADIFI*VTMAVN	-1.848	0.002841	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23489	YDAPRTFKVDC			11	SLAY-screened peptide P1839			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGACGCTCCTCGCACTTTCAAGGTCGATTGCTAGACGAGTCACAACCAGAGCCCCTTCTAA	YDAPRTFKVDC*TSHNQSPF*	-1.848	0.045375	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23490	LPCPPSWATSMNCPFVASQA			20	SLAY-screened peptide P1840			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCGTGCCCCCCTAGCTGGGCTACCTCCATGAATTGCCCGTTTGTGGCTAGCCAGGCCTAA	LPCPPSWATSMNCPFVASQA*	-1.845	0.000509	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23491	HQRSDKCVATSDAYLSSKPT			20	SLAY-screened peptide P1841			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCAGCGGTCCGACAAGTGTGTTGCCACTTCGGATGCTTATCTTAGCTCCAAGCCTACCTAA	HQRSDKCVATSDAYLSSKPT*	-1.845	0.002404	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23492	HVFEPFDNLPMNPIWSNQ			18	SLAY-screened peptide P1842			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGTTTTCGAGCCCTTCGACAATCTTCCGATGAATCCTATTTGGTCTAACCAGTAGAGCTAA	HVFEPFDNLPMNPIWSNQ*S*	-1.845	0.000601	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23493	LNFSAVRQDGTHRTSSNSNH			20	SLAY-screened peptide P1843			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGAATTTCTCCGCTGTCCGGCAGGATGGCACTCACCGTACTTCGAGTAACTCTAATCATTAA	LNFSAVRQDGTHRTSSNSNH*	-1.844	0.000216	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23494	THHKNWMSSYPTVQNLIYYT			20	SLAY-screened peptide P1844			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCATCACAAGAATTGGATGTCCTCTTATCCGACTGTTCAGAATCTGATTTACTACACCTAA	THHKNWMSSYPTVQNLIYYT*	-1.844	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23495	SALAVVTSRTSCTYTPN			17	SLAY-screened peptide P1845			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGCGCTGGCCGTCGTTACTTCCCGTACTTCTTGCACTTATACTCCGAACTAGTCTCTGTAA	SALAVVTSRTSCTYTPN*SL*	-1.844	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23496	KTITLLLVAYNSNNIS			16	SLAY-screened peptide P1846			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGACTATTACCCTCCTTTTGGTTGCTTACAATTCGAACAATATTTCCTAGGTCACGAGGTAA	KTITLLLVAYNSNNIS*VTR*	-1.844	0.00062	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23497	NILCTGSSRRFMLIIARTTCN			21	SLAY-screened peptide P1847			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACATCCTTTGTACTGGTAGTTCGCGTCGCTTCATGCTTATTATTGCGCGTACTACATGTAAC	NILCTGSSRRFMLIIARTTCN	-1.843	0.001629	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23498	RY			2	SLAY-screened peptide P1848			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTATTAGGTGCGTAGGCCCAACCACATGGGTCATTACGATGTTTTTGATTTCATGAACTAA	RY*VRRPNHMGHYDVFDFMN*	-1.843	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23499	SYPDQVRPHNPHGILSVDNS			20	SLAY-screened peptide P1849			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTACCCCGACCAGGTTAGGCCGCACAATCCTCATGGTATTCTCAGTGTTGATAACAGCTAA	SYPDQVRPHNPHGILSVDNS*	-1.843	0.000118	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23500	TYHIAISSSNALLTAALPRG			20	SLAY-screened peptide P1850			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTATCATATTGCTATCTCCTCTAGTAATGCCCTGCTTACCGCTGCGTTGCCTCGTGGTTAA	TYHIAISSSNALLTAALPRG*	-1.843	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23501	NRVAEVKDNDNGLVTATVAFN			21	SLAY-screened peptide P1851			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCGGGTTGCTGAGGTTAAGGATAATGACAACGGCCTGGTTACTGCGACCGTGGCTTTTAAC	NRVAEVKDNDNGLVTATVAFN	-1.842	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23502	GRSHLATFDPHDWHIRLPILN			21	SLAY-screened peptide P1852			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCGCTCTCATCTGGCTACGTTTGACCCCCACGATTGGCATATTAGGTTGCCGATACTTAAC	GRSHLATFDPHDWHIRLPILN	-1.841	0.000608	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23503	PHSSFDAADYYTGQSCVKCY			20	SLAY-screened peptide P1853			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCATTCGTCCTTCGACGCCGCGGACTACTATACTGGTCAGTCGTGCGTGAAGTGTTATTAA	PHSSFDAADYYTGQSCVKCY*	-1.841	0.001893	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23504	YTPFHSFPIWPCPPSLHLAV			20	SLAY-screened peptide P1854			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACGCCCTTTCATTCGTTTCCTATCTGGCCTTGTCCTCCCAGCCTGCACCTCGCGGTTTAA	YTPFHSFPIWPCPPSLHLAV*	-1.841	0.013543	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23505	SHGLGDKPSPHNNSNIVCPN			20	SLAY-screened peptide P1855			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCATGGTCTCGGTGACAAGCCGTCGCCGCACAACAACTCTAATATTGTTTGCCCTAATTAA	SHGLGDKPSPHNNSNIVCPN*	-1.841	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23506	RYYPRNWRHLYNNVRTHLTS			20	SLAY-screened peptide P1856			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTATTATCCTCGGAACTGGCGGCACCTGTATAATAATGTCCGGACCCATCTTACTTCCTAA	RYYPRNWRHLYNNVRTHLTS*	-1.84	6.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23507	ILSSNQLMCCVPRSSLIHRL			20	SLAY-screened peptide P1857			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCTCTCGTCTAACCAGCTTATGTGTTGTGTCCCGCGTAGTAGTCTTATTCACAGGCTTTAA	ILSSNQLMCCVPRSSLIHRL*	-1.839	0.001689	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23508	RTIARLLYAFLRRVSACSGM			20	SLAY-screened peptide P1858			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGACCATCGCTCGTCTGCTCTATGCTTTCCTTCGTCGGGTTAGCGCGTGCTCCGGTATGTAA	RTIARLLYAFLRRVSACSGM*	-1.839	0.012215	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23509	PRYLTDFLLRPADSQYSDPH			20	SLAY-screened peptide P1859			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGCTACCTGACCGACTTCTTGCTGCGGCCTGCTGATTCCCAGTACTCCGACCCGCATTAA	PRYLTDFLLRPADSQYSDPH*	-1.839	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23510	FKTIHMHGVSVTPMG			15	SLAY-screened peptide P1860			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTAAGACTATTCACATGCACGGTGTATCTGTTACACCTATGGGTTGATTTATATGAAGTAAC	FKTIHMHGVSVTPMG*FI*SN	-1.838	0.000697	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23511	LLNPFNDRDSMPLSHFYMLL			20	SLAY-screened peptide P1861			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCTTAACCCGTTCAATGACCGCGATTCCATGCCTCTTAGTCACTTTTACATGCTGCTGTAA	LLNPFNDRDSMPLSHFYMLL*	-1.838	0.000171	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23512	GAR			3	SLAY-screened peptide P1862			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTGCCCGCTAGGGCATTTTTAGCTGCACCAATAATAACGAGGTCTGTTATTCTTATTGCTAA	GAR*GIFSCTNNNEVCYSYC*	-1.838	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23513	CFDRPHNFINGIPFLRIYTT			20	SLAY-screened peptide P1863			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTTCGATCGTCCCCACAACTTCATTAACGGTATTCCCTTTTTGCGCATTTACACTACCTAA	CFDRPHNFINGIPFLRIYTT*	-1.838	2.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23514	MNKHPAPGCPGHAHRSLKFW			20	SLAY-screened peptide P1864			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGAACAAGCACCCTGCTCCCGGCTGTCCCGGCCACGCGCATCGTTCTCTGAAGTTTTGGTAA	MNKHPAPGCPGHAHRSLKFW*	-1.837	0.000622	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23515	LVINMLCKTIQLFKLIQLVF			20	SLAY-screened peptide P1865			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGTGATTAATATGCTTTGCAAGACCATTCAGCTTTTCAAGCTCATTCAGCTTGTGTTTTAA	LVINMLCKTIQLFKLIQLVF*	-1.837	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23516	VSTPSPLFGKDPHWWTQALR			20	SLAY-screened peptide P1866			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTCTACCCCTAGCCCTCTGTTTGGCAAGGATCCGCATTGGTGGACCCAGGCGCTCCGTTAA	VSTPSPLFGKDPHWWTQALR*	-1.836	0.003219	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23517	VSSFLLSTYGGLRMHTFIPV			20	SLAY-screened peptide P1867			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTCCAGCTTTTTGCTCTCCACGTATGGTGGCCTGCGTATGCACACCTTCATCCCCGTGTAA	VSSFLLSTYGGLRMHTFIPV*	-1.836	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23518	FEGRQAAD			8	SLAY-screened peptide P1868			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGAGGGGCGCCAGGCTGCGGATTAGGCTCATTGCTGCAATATGCATGCCCCTGACACTTAA	FEGRQAAD*AHCCNMHAPDT*	-1.836	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23519	YTAPRADILKKRLNNSLDTV			20	SLAY-screened peptide P1869			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATACTGCGCCGCGGGCGGACATCCTGAAGAAGCGCTTGAACAATTCGTTGGATACCGTGTAA	YTAPRADILKKRLNNSLDTV*	-1.835	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23520	SRLKVPRGHPLAPYDRGMMA			20	SLAY-screened peptide P1870			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCGCCTCAAGGTCCCCCGGGGGCACCCGCTGGCGCCGTATGATCGCGGCATGATGGCGTAA	SRLKVPRGHPLAPYDRGMMA*	-1.834	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23521	INIPKLILILLISADHHQMP			20	SLAY-screened peptide P1871			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAACATTCCTAAGCTTATTCTCATTCTTTTGATCTCTGCGGACCACCACCAGATGCCCTAA	INIPKLILILLISADHHQMP*	-1.834	0.000583	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23522	NTFNTLGCFFSDPMP			15	SLAY-screened peptide P1872			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATACCTTTAATACCTTGGGCTGTTTCTTCTCTGACCCAATGCCGTGATGACTACGCTCTAAC	NTFNTLGCFFSDPMP**LRSN	-1.834	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23523	LCPSQHPLYFALAGTPITLLN			21	SLAY-screened peptide P1873			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTGTCCTTCCCAGCACCCTCTGTACTTCGCGTTAGCCGGCACTCCGATAACGCTCCTTAAC	LCPSQHPLYFALAGTPITLLN	-1.834	0.011689	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23524	VHRRPITSYNPWDCYFLHHY			20	SLAY-screened peptide P1874			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCACCGCCGTCCTATCACTTCGTATAACCCCTGGGACTGCTATTTCCTTCACCATTATTAA	VHRRPITSYNPWDCYFLHHY*	-1.833	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23525	GTGFFVGINISNPPRLYVAW			20	SLAY-screened peptide P1875			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTACGGGCTTTTTTGTGGGCATCAATATCTCTAATCCCCCCAGGCTTTATGTTGCGTGGTAA	GTGFFVGINISNPPRLYVAW*	-1.832	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23526	SVHSRLPDVYGSYSPSSGGT			20	SLAY-screened peptide P1876			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGTCCACTCCAGGCTGCCCGATGTTTATGGGAGCTACTCCCCTAGTTCTGGGGGTACGTAA	SVHSRLPDVYGSYSPSSGGT*	-1.831	0.011554	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23527	WIPKRPELRGCSYGNPPESF			20	SLAY-screened peptide P1877			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGATTCCCAAGCGCCCGGAGTTGAGGGGCTGTTCTTACGGGAATCCTCCCGAGAGTTTCTAA	WIPKRPELRGCSYGNPPESF*	-1.831	0.00787	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23528	ILNENNQPWNRCVIYYLAQR			20	SLAY-screened peptide P1878			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTTGAACGAGAATAACCAGCCGTGGAACCGCTGCGTCATTTATTACCTGGCCCAGCGTTAA	ILNENNQPWNRCVIYYLAQR*	-1.831	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23529	RIQPVHDR			8	SLAY-screened peptide P1879			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATTCAGCCTGTTCATGATCGCTAGTGGATTAATTACGGCCAGAGCATGCTTGATTCCTAA	RIQPVHDR*WINYGQSMLDS*	-1.831	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23530	YFPLCFRVNRSASSHRRFRR			20	SLAY-screened peptide P1880			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTTTCCTCTCTGCTTCCGTGTCAATCGTTCGGCTAGTTCCCATCGTCGTTTTCGTAGGTAA	YFPLCFRVNRSASSHRRFRR*	-1.831	0.030331	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23531	ISVVPTYRNKFDCTLCHHKY			20	SLAY-screened peptide P1881			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTTCCGTGGTGCCTACGTATAGGAACAAGTTCGACTGCACTTTGTGTCATCACAAGTACTAA	ISVVPTYRNKFDCTLCHHKY*	-1.831	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23532	SCGHHFGTTNKANPRCYVVC			20	SLAY-screened peptide P1882			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTGTGGGCATCACTTTGGTACCACGAATAAGGCTAATCCGAGGTGTTATGTTGTCTGTTAA	SCGHHFGTTNKANPRCYVVC*	-1.83	0.000521	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23533	RHTYPSPISRVGSPRARTWI			20	SLAY-screened peptide P1883			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCACACTTACCCTAGTCCTATCTCTAGGGTGGGCTCCCCCCGTGCTCGGACTTGGATTTAA	RHTYPSPISRVGSPRARTWI*	-1.83	0.011328	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23534	WQIPPGCRIARA			12	SLAY-screened peptide P1884			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCAGATTCCTCCCGGTTGCCGTATCGCTCGGGCTTAGGTTTTCTCCCTTATCGAGCCCTAA	WQIPPGCRIARA*VFSLIEP*	-1.83	0.014309	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23535	YKSSKHRITQPNSTVQTCLR			20	SLAY-screened peptide P1885			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAAGTCCAGCAAGCACAGGATTACTCAGCCCAACTCGACTGTTCAGACTTGCTTGAGGTAA	YKSSKHRITQPNSTVQTCLR*	-1.829	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23536	LPLHGVADNHRDIARAVPI			19	SLAY-screened peptide P1886			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCTCTCCACGGGGTTGCGGACAACCACAGGGATATCGCTCGTGCCGTCCCTATCTAGTAA	LPLHGVADNHRDIARAVPI**	-1.829	5.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23537	SIIDSEPTDRYYDRFYYYIF			20	SLAY-screened peptide P1887			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCATTATTGACTCCGAGCCTACTGATCGCTATTATGACCGCTTTTACTACTATATCTTCTAA	SIIDSEPTDRYYDRFYYYIF*	-1.828	0.000225	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23538	PNRAVRGFANISTTIPIGR			19	SLAY-screened peptide P1888			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAACCGGGCTGTGAGGGGCTTTGCCAATATTTCTACGACTATTCCGATTGGCCGCTAGTAA	PNRAVRGFANISTTIPIGR**	-1.828	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23539	RTPRPPRVPTIDPYFLMDMY			20	SLAY-screened peptide P1889			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACCCCGCGGCCCCCCCGTGTGCCTACTATTGATCCGTATTTTCTTATGGATATGTATTAA	RTPRPPRVPTIDPYFLMDMY*	-1.828	0.007071	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23540	RLPYKFFSGIIRCAVWIIYP			20	SLAY-screened peptide P1890			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTGCCCTATAAGTTCTTCTCGGGTATTATTCGCTGTGCCGTGTGGATTATTTATCCCTAA	RLPYKFFSGIIRCAVWIIYP*	-1.828	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23541	PLVCSRCAVTVMSCL			15	SLAY-screened peptide P1891			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTGTCTGCTCGCGTTGCGCCGTGACCGTAATGTCGTGTTTGTGAGCTTTACCTCTTAAC	PLVCSRCAVTVMSCL*ALPLN	-1.828	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23542	QTSHNTWDHFSSSIHVSDNL			20	SLAY-screened peptide P1892			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGACCTCTCACAATACCTGGGATCATTTTTCTTCGTCTATTCATGTTTCTGATAACCTTTAA	QTSHNTWDHFSSSIHVSDNL*	-1.827	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23543	TRASHARHWHPHLLYYQGTL			20	SLAY-screened peptide P1893			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCGCGCTTCCCATGCCCGCCACTGGCACCCCCACCTTCTGTATTACCAGGGGACTCTTTAA	TRASHARHWHPHLLYYQGTL*	-1.827	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23544	SLLVLMISPTVRRTGFTRSSN			21	SLAY-screened peptide P1894			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTTGTTGGTGCTGATGATTAGTCCCACGGTTCGTCGGACTGGTTTTACACGATCGTCTAAC	SLLVLMISPTVRRTGFTRSSN	-1.827	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23545	PPTRRSTTRATAGYDAPAHL			20	SLAY-screened peptide P1895			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCCACTAGGCGGTCCACGACGCGCGCCACCGCCGGCTACGACGCGCCGGCTCACCTCTAA	PPTRRSTTRATAGYDAPAHL*	-1.826	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23546	NKEPVDHSTEGNQNLDYSSC			20	SLAY-screened peptide P1896			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACAAGGAGCCGGTCGACCACAGTACGGAGGGCAATCAGAATCTGGACTATAGTTCGTGCTAA	NKEPVDHSTEGNQNLDYSSC*	-1.826	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23547	GEAPDVADSICSLHWGL			17	SLAY-screened peptide P1897			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGAGGCTCCCGATGTTGCTGATTCTATCTGTTCTTTGCACTGGGGGCTTTAGACCATTTAA	GEAPDVADSICSLHWGL*TI*	-1.825	0.004574	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23548	ATRDDSE			7	SLAY-screened peptide P1898			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTACTCGCGATGACTCGGAGTAGCCAGCCGGACTCCTACCTCGAAGTATCAGTGTTTTTAAC	ATRDDSE*PAGLLPRSISVFN	-1.824	0.019832	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23549	RALSLLCTLRRLFLPSI			17	SLAY-screened peptide P1899			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGCCCTCAGCCTTCTTTGTACCCTTAGGCGTTTGTTTCTTCCCTCTATTTAGAAGATGTAA	RALSLLCTLRRLFLPSI*KM*	-1.824	0.002262	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23550	YASWRSAAYFDIMGVFYSLP			20	SLAY-screened peptide P1900			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGCTTCGTGGCGGTCCGCTGCTTACTTCGACATCATGGGCGTCTTTTACAGTCTCCCCTAA	YASWRSAAYFDIMGVFYSLP*	-1.823	0.001628	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23551	LWGVSCSPRGNPCRCLGLDA			20	SLAY-screened peptide P1901			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTGGGGCGTCTCTTGCAGCCCGCGTGGTAACCCGTGCCGTTGTCTTGGTTTGGACGCTTAA	LWGVSCSPRGNPCRCLGLDA*	-1.823	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23552	RAHLITLSFVLLASEEQYDR			20	SLAY-screened peptide P1902			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGGCCCACCTTATCACCTTGTCCTTTGTTCTCCTTGCCAGCGAGGAGCAGTATGACCGGTAA	RAHLITLSFVLLASEEQYDR*	-1.823	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23553	ARGWMPYGHYMSVAQTANYM			20	SLAY-screened peptide P1903			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCGCGGCTGGATGCCTTATGGTCACTACATGTCCGTGGCGCAGACCGCGAATTACATGTAA	ARGWMPYGHYMSVAQTANYM*	-1.823	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23554	PPFILVVCHYL			11	SLAY-screened peptide P1904			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCTTTATTCTGGTTGTTTGCCATTACTTGTAGCCCGCCACCTGGATCAGCTCGCGCTAA	PPFILVVCHYL*PATWISSR*	-1.823	0.012081	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23555	LDSFPGHSTYPRHPLHCVLL			20	SLAY-screened peptide P1905			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGACAGCTTCCCTGGTCATTCCACGTATCCCAGGCACCCCCTCCACTGTGTCCTCCTTTAA	LDSFPGHSTYPRHPLHCVLL*	-1.822	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23556	STSALHIIAFGSPPRAFSRGN			21	SLAY-screened peptide P1906			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTACGTCCGCTCTCCATATCATTGCATTTGGATCTCCTCCGCGAGCTTTTTCCCGCGGTAAC	STSALHIIAFGSPPRAFSRGN	-1.822	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23557	SSTNHFIYYIGLLHLH			16	SLAY-screened peptide P1907			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTCTACTAACCACTTTATCTACTATATCGGCTTGCTCCACCTCCACTAGCTCGATCGTTAA	SSTNHFIYYIGLLHLH*LDR*	-1.821	0.006986	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23558	LEDDNPQALLYA			12	SLAY-screened peptide P1908			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGAGGACGATAATCCGCAGGCGCTGCTGTACGCCTAGTACCTTCACTGTTTCTGGTACTAA	LEDDNPQALLYA*YLHCFWY*	-1.821	0.000176	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23559	APWNAYHLSDFRTQSHRLST			20	SLAY-screened peptide P1909			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCGTGGAACGCTTACCACCTCTCCGATTTTCGCACTCAGTCGCATCGCCTGTCCACGTAA	APWNAYHLSDFRTQSHRLST*	-1.82	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23560	FTMSSDNSNSKFNRSYFHQR			20	SLAY-screened peptide P1910			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCACGATGTCTAGTGATAACTCCAACAGTAAGTTTAATCGTAGCTATTTCCATCAGAGGTAA	FTMSSDNSNSKFNRSYFHQR*	-1.82	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23561	PL			2	SLAY-screened peptide P1911			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTGTAGGCTAATGCGCGTCCCGATGTGGATGACTCGTCCAGTCAGATCGATGGGGACTAA	PL*ANARPDVDDSSSQIDGD*	-1.82	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23562	GFQCYCPLNIHVI			13	SLAY-screened peptide P1912			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTTTCAGTGCTACTGTCCCCTGAACATCCACGTTATTTAGCCTGTTGGTTTGTTTCATTAA	GFQCYCPLNIHVI*PVGLFH*	-1.819	0.007971	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23563	RML			3	SLAY-screened peptide P1913			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATGCTTTAGTCGGACTACATCATTAAGCGGGCCACGTCGAATATGCAGAACAATTTCTAA	RML*SDYIIKRATSNMQNNF*	-1.819	0.00157	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23564	PGATDPWRLVSHRFRHSGRP			20	SLAY-screened peptide P1914			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGGCGCCACCGATCCTTGGCGTCTGGTGTCCCATCGCTTCCGTCACAGTGGTCGGCCGTAA	PGATDPWRLVSHRFRHSGRP*	-1.819	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23565	PRGPECPIVTSLLVRVTYSS			20	SLAY-screened peptide P1915			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGGGGCCCTGAGTGTCCGATTGTTACTTCTCTCCTTGTTAGGGTCACTTATTCCTCTTAA	PRGPECPIVTSLLVRVTYSS*	-1.819	0.009443	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23566	VLFYLRLGRTIVVTYNLSNT			20	SLAY-screened peptide P1916			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCTTTTCTACTTGCGCCTGGGCCGGACTATCGTTGTCACGTATAATCTCTCTAATACCTAA	VLFYLRLGRTIVVTYNLSNT*	-1.819	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23567	MTHTPVYSDVLHLPPSLSCVT			21	SLAY-screened peptide P1917			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGACCCACACCCCAGTTTATTCAGACGTACTTCATTTACCACCATCCTTGTCGTGCGTAACT	MTHTPVYSDVLHLPPSLSCVT	-1.819	8.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23568	STNFPERTVWHHMSSASTNS			20	SLAY-screened peptide P1918			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGACTAACTTCCCCGAGCGGACCGTCTGGCATCACATGTCTTCTGCTAGTACCAACAGCTAA	STNFPERTVWHHMSSASTNS*	-1.817	0.015958	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23569	LIQTEPQSTSLAYSATFGSY			20	SLAY-screened peptide P1919			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCATTCAGACTGAGCCCCAGTCGACCAGCCTGGCCTATAGTGCGACCTTTGGGTCCTATTAA	LIQTEPQSTSLAYSATFGSY*	-1.817	0.003589	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23570	MSACSSPVNFYRQTCNSYKA			20	SLAY-screened peptide P1920			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTCCGCGTGCTCTTCTCCTGTCAATTTTTATCGCCAGACTTGTAATAGTTACAAGGCTTAA	MSACSSPVNFYRQTCNSYKA*	-1.817	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23571	RRNSFLRCLTGRVGSTHVNP			20	SLAY-screened peptide P1921			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGGAACTCTTTCCTCAGGTGTCTTACGGGTCGCGTTGGCTCGACTCACGTCAATCCGTAA	RRNSFLRCLTGRVGSTHVNP*	-1.816	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23572	PSHAPPTLPATTV			13	SLAY-screened peptide P1922			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCCCATGCCCCTCCGACGCTCCCTGCGACCACTGTTTAGGACACTCCGCACTCGCGCTAA	PSHAPPTLPATTV*DTPHSR*	-1.816	0.00051	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23573	DGYYFWHIDC			10	SLAY-screened peptide P1923			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATGGCTATTATTTCTGGCACATCGATTGTTAGACGCAGGCTATGTATACTGAGGGCAACTAA	DGYYFWHIDC*TQAMYTEGN*	-1.815	0.00034	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23574	ESTCYLPSCHPSPAVRWGSA			20	SLAY-screened peptide P1924			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGAGCACCTGCTACCTCCCCAGCTGTCACCCGTCTCCGGCGGTCAGGTGGGGTTCTGCCTAA	ESTCYLPSCHPSPAVRWGSA*	-1.815	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23575	SGIS			4	SLAY-screened peptide P1925			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGGTATTTCGTAGGCTCGCAGCCCGATGGCGGTTACTCTTGTTTCGTACCCGCTTACTTAA	SGIS*ARSPMAVTLVSYPLT*	-1.815	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23576	YVRTNAPQLAIFSAYPAHVF			20	SLAY-screened peptide P1926			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGTGCGCACTAACGCCCCCCAGTTGGCTATCTTCAGCGCTTATCCTGCGCATGTGTTCTAA	YVRTNAPQLAIFSAYPAHVF*	-1.815	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23577	PPNTATCLPTPFPYAKLRYT			20	SLAY-screened peptide P1927			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCGAACACTGCCACGTGCCTTCCTACTCCTTTTCCTTATGCGAAGCTTCGTTATACGTAA	PPNTATCLPTPFPYAKLRYT*	-1.815	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23578	HANYASCFARGETSSPCNHN			20	SLAY-screened peptide P1928			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGCTAATTATGCTTCTTGTTTTGCCAGGGGCGAGACGTCGAGTCCGTGTAACCACAATTAA	HANYASCFARGETSSPCNHN*	-1.815	0.0122	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23579	VCKWITTWKQILSPNKHLRP			20	SLAY-screened peptide P1929			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTGCAAGTGGATTACTACCTGGAAGCAGATTCTCTCCCCGAATAAGCATTTGCGTCCCTAA	VCKWITTWKQILSPNKHLRP*	-1.815	0.013632	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23580	PPTHYCPNWYRIFRQPLLVL			20	SLAY-screened peptide P1930			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTACTCATTATTGTCCTAACTGGTACCGCATCTTTCGTCAGCCCTTGCTCGTTCTGTAA	PPTHYCPNWYRIFRQPLLVL*	-1.814	0.001752	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23581	SSSTMQCYELMFQ			13	SLAY-screened peptide P1931			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTCTTCTACCATGCAGTGCTACGAGCTCATGTTCCAGTAGACCCGTATTTGGGTCATTTAA	SSSTMQCYELMFQ*TRIWVI*	-1.814	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23582	PSPFPNEPSDSPPFCYLLVA			20	SLAY-screened peptide P1932			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGCCCCTTCCCCAACGAGCCGTCCGATTCGCCGCCGTTCTGCTATCTCCTGGTTGCGTAA	PSPFPNEPSDSPPFCYLLVA*	-1.814	0.002075	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23583	QSVPKRRSNPTIQDLSRNCS			20	SLAY-screened peptide P1933			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTCCGTCCCGAAGAGGCGCAGTAATCCTACCATTCAGGATCTGTCGCGCAACTGCTCGTAA	QSVPKRRSNPTIQDLSRNCS*	-1.814	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23584	PAHTPNLLTDSFTHTRMNIT			20	SLAY-screened peptide P1934			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCCCATACCCCTAATCTTCTCACCGATTCGTTTACCCATACGAGGATGAATATTACCTAA	PAHTPNLLTDSFTHTRMNIT*	-1.814	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23585	IR			2	SLAY-screened peptide P1935			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCGGTAGCGCTAGTACGAGATCCCGGGGCGTTACATTAATATCAGCAGGCTTTTTAATTAA	IR*R*YEIPGRYINISRLFN*	-1.813	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23586	PVTMDSPTPDQRVTAHFALT			20	SLAY-screened peptide P1936			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGTTACCATGGATTCCCCTACGCCGGATCAGCGCGTTACTGCGCATTTTGCTCTCACCTAA	PVTMDSPTPDQRVTAHFALT*	-1.813	0.016429	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23587	CTNLGVRHLTDTMGRRVISFN			21	SLAY-screened peptide P1937			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCACGAATCTTGGCGTTCGTCATCTTACTGACACTATGGGCAGGAGAGTGATATCGTTTAAC	CTNLGVRHLTDTMGRRVISFN	-1.813	0.007014	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23588	YTSGFCLPIHEMA			13	SLAY-screened peptide P1938			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACGTCGGGCTTCTGCCTCCCGATCCATGAGATGGCCTAGCCCCCCTACCATAAGGAGTAA	YTSGFCLPIHEMA*PPYHKE*	-1.812	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23589	PSTCSFYQCEGCSNIIGLDG			20	SLAY-screened peptide P1939			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGTACCTGTAGCTTTTATCAGTGTGAGGGTTGCTCTAATATTATTGGTCTGGATGGCTAA	PSTCSFYQCEGCSNIIGLDG*	-1.812	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23590	ADLLPRSHYNSYMHIEEFFI			20	SLAY-screened peptide P1940			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGACTTGCTGCCTCGTTCGCACTACAACTCGTATATGCATATCGAGGAGTTTTTCATCTAA	ADLLPRSHYNSYMHIEEFFI*	-1.812	0.022619	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23591	AIALPSGQHGIPHVIY			16	SLAY-screened peptide P1941			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCATTGCTCTTCCCTCCGGCCAGCACGGCATTCCTCATGTCATTTATTAGAAGACCTGGTAA	AIALPSGQHGIPHVIY*KTW*	-1.811	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23592	YSRVIRITLPDTGFGPCHDF			20	SLAY-screened peptide P1942			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTCCCGGGTGATTAGGATTACGTTGCCGGACACTGGTTTTGGGCCTTGCCATGACTTCTAA	YSRVIRITLPDTGFGPCHDF*	-1.811	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23593	PHPRDDEDHFHRNNSLPA			18	SLAY-screened peptide P1943			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCACCCGCGGGATGATGAGGATCACTTCCATCGTAATAATAGCCTTCCCGCTTAACGTAAC	PHPRDDEDHFHRNNSLPA*RN	-1.811	0.001299	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23594	LNNRNRSLPGNPTIEYFVVE			20	SLAY-screened peptide P1944			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAATAACCGGAACCGCTCGCTGCCCGGGAACCCGACTATTGAGTATTTCGTTGTGGAGTAA	LNNRNRSLPGNPTIEYFVVE*	-1.811	0.00027	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23595	HSTWVAGSYYAIDRSFGSSL			20	SLAY-screened peptide P1945			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTCCACGTGGGTGGCTGGCTCGTATTACGCTATTGATAGGTCCTTTGGCTCTTCCCTCTAA	HSTWVAGSYYAIDRSFGSSL*	-1.809	0.000984	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23596	LVRDTTFITIRGDNTERALV			20	SLAY-screened peptide P1946			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGTCCGCGACACCACCTTTATCACGATCCGCGGTGATAATACCGAGAGGGCCTTGGTGTAA	LVRDTTFITIRGDNTERALV*	-1.809	0.000436	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23597	TVLPYRPHWADTDNNFVLQN			20	SLAY-screened peptide P1947			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTCCTTCCTTATCGCCCTCACTGGGCGGATACGGATAATAACTTTGTTCTGCAGAACTAA	TVLPYRPHWADTDNNFVLQN*	-1.809	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23598	STSTE			5	SLAY-screened peptide P1948			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGACGTCTACGGAGTAGGCCACTCAGCACCGGTGCATCGCCTTCGTGTCCTACCATTGTTAA	STSTE*ATQHRCIAFVSYHC*	-1.809	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23599	IRHG			4	SLAY-screened peptide P1949			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAGGCACGGCTAGATTTTCGCTCCCGTTACTAATATTCACTATAGGAATAATATTGCTAAC	IRHG*IFAPVTNIHYRNNIAN	-1.809	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23600	NLIKGFARLFVCLFQSRFCR			20	SLAY-screened peptide P1950			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCTTATCAAGGGCTTCGCTCGTCTTTTCGTTTGTCTTTTCCAGTCCCGGTTTTGCCGGTAA	NLIKGFARLFVCLFQSRFCR*	-1.808	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23601	CNTTFPPYFILPILHRNHHM			20	SLAY-screened peptide P1951			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAATACCACCTTTCCCCCGTATTTCATTCTGCCCATTTTGCATCGCAACCATCATATGTAA	CNTTFPPYFILPILHRNHHM*	-1.808	0.023565	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23602	VSSMGPTLLTSLSGQLKQTY			20	SLAY-screened peptide P1952			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTCTAGTATGGGCCCTACCCTGCTCACTAGTCTCTCCGGCCAGCTCAAGCAGACCTATTAA	VSSMGPTLLTSLSGQLKQTY*	-1.808	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23603	WALAIYSLAHNVDESDWRTL			20	SLAY-screened peptide P1953			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGGCCCTTGCTATTTACAGTCTCGCTCATAACGTTGATGAGTCGGACTGGCGCACTCTTTAA	WALAIYSLAHNVDESDWRTL*	-1.807	9.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23604	HSYNRGRPKNSTYS			14	SLAY-screened peptide P1954			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAGCTACAATAGGGGTCGTCCTAAGAATAGCACCTACTCTTAGGTTAACCCTTTTCAGTAA	HSYNRGRPKNSTYS*VNPFQ*	-1.807	0.000138	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23605	PWSPPWVILRPAILLFASTRN			21	SLAY-screened peptide P1955			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGGTCCCCACCCTGGGTTATCCTCAGGCCCGCCATTCTACTGTTTGCAAGCACGCGTAAC	PWSPPWVILRPAILLFASTRN	-1.807	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23606	DLSPCLTAPPVTTLDPLAV			19	SLAY-screened peptide P1956			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCTCAGTCCCTGTCTTACGGCCCCTCCCGTTACGACCCTGGACCCTCTCGCGGTGTAGTAA	DLSPCLTAPPVTTLDPLAV**	-1.806	0.00163	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23607	TAASLSSVANSQLLSENAFT			20	SLAY-screened peptide P1957			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGCTGCCAGCCTCTCCTCCGTGGCTAACTCCCAGCTGCTTTCGGAGAACGCTTTCACGTAA	TAASLSSVANSQLLSENAFT*	-1.806	0.006977	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23608	THIDQISPCYLHVSDS			16	SLAY-screened peptide P1958			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCACATTGACCAGATTTCCCCCTGCTATCTGCATGTGAGTGATTCTTAGCAGCACGCCTAA	THIDQISPCYLHVSDS*QHA*	-1.806	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23609	DICQAHNDRQS			11	SLAY-screened peptide P1959			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACATTTGTCAGGCCCACAACGATAGGCAGAGCTAGTAGCGGAAGTGCTTCCGGGATAACTAA	DICQAHNDRQS**RKCFRDN*	-1.806	0.006853	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23610	DDSTKDPYRLLCFRNYSMNL			20	SLAY-screened peptide P1960			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGACTCCACCAAGGACCCCTACCGTCTCCTGTGCTTTCGCAACTACTCTATGAATCTTTAA	DDSTKDPYRLLCFRNYSMNL*	-1.806	0.001398	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23611	TPRTFLTRTLTRMCNYVVDH			20	SLAY-screened peptide P1961			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCTCGGACCTTCCTTACCAGGACCCTTACGCGTATGTGCAATTACGTTGTCGATCACTAA	TPRTFLTRTLTRMCNYVVDH*	-1.805	0.00012	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23612	SREPHSIHNNTCWQATLMIA			20	SLAY-screened peptide P1962			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCGCGAGCCCCACTCGATCCACAACAATACCTGTTGGCAGGCGACGCTTATGATTGCCTAA	SREPHSIHNNTCWQATLMIA*	-1.805	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23613	RLVGPDSLGTHSLAQYPTRP			20	SLAY-screened peptide P1963			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTTGTGGGTCCCGACAGCCTTGGTACGCATTCTCTGGCTCAGTACCCTACCAGGCCGTAA	RLVGPDSLGTHSLAQYPTRP*	-1.805	0.017147	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23614	HAILLIYLTLLIPSRTSARE			20	SLAY-screened peptide P1964			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCCATCTTGCTTATCTATCTTACCCTGCTCATCCCTTCGCGGACTTCCGCCCGCGAGTAA	HAILLIYLTLLIPSRTSARE*	-1.804	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23615	NQRFRSIHARLTGQNYWMLN			20	SLAY-screened peptide P1965			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCAGCGCTTCCGGTCCATTCACGCTCGGCTTACTGGCCAGAACTATTGGATGTTGAACTAA	NQRFRSIHARLTGQNYWMLN*	-1.804	0.001502	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23616	PMVEFHVPLAYRTTDENHRV			20	SLAY-screened peptide P1966			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATGGTGGAGTTTCATGTTCCCCTTGCCTACAGGACCACCGACGAGAATCACCGCGTTTAA	PMVEFHVPLAYRTTDENHRV*	-1.804	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23617	ILYWRPNRSTWAFTVTPIMV			20	SLAY-screened peptide P1967			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCTCTACTGGCGTCCCAACCGCTCCACTTGGGCCTTCACCGTTACTCCCATCATGGTTTAA	ILYWRPNRSTWAFTVTPIMV*	-1.804	0.00586	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23618	ANSHHLYHLDHGFHNPTIRCN			21	SLAY-screened peptide P1968			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTAATAGTCATCACCTGTATCATCTTGACCACGGGTTTCACAATCCAACTATAAGGTGTAAC	ANSHHLYHLDHGFHNPTIRCN	-1.804	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23619	CYTPLYMFAVNLITDFSTIA			20	SLAY-screened peptide P1969			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTACACTCCTCTGTATATGTTCGCCGTTAATCTTATCACGGACTTCTCGACGATCGCCTAA	CYTPLYMFAVNLITDFSTIA*	-1.804	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23620	LWENPSIHFVSGFSVSITYL			20	SLAY-screened peptide P1970			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTGGGAGAATCCTTCCATCCACTTCGTTTCCGGGTTCTCGGTGTCTATTACCTACCTTTAA	LWENPSIHFVSGFSVSITYL*	-1.804	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23621	KTWEVLTEFRLYTNILWTWD			20	SLAY-screened peptide P1971			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGACCTGGGAGGTTCTCACTGAGTTTCGGCTTTATACTAATATTCTTTGGACGTGGGATTAA	KTWEVLTEFRLYTNILWTWD*	-1.803	5.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23622	HQRPSNMDC			9	SLAY-screened peptide P1972			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCAGAGGCCCTCCAATATGGATTGCTAGGCCAGGCGTCAGGAGGTCCGCCGTAGTAGTTAA	HQRPSNMDC*ARRQEVRRSS*	-1.803	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23623	TVAHLFNITNIHTNNLT			17	SLAY-screened peptide P1973			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTTGCCCACCTTTTTAACATTACGAATATCCACACGAACAATCTTACGTAGGACTCGTAA	TVAHLFNITNIHTNNLT*DS*	-1.803	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23624	LNDKDKTYAVLHIPYNWTPT			20	SLAY-screened peptide P1974			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAATGACAAGGACAAGACTTATGCGGTTCTCCACATTCCCTACAATTGGACGCCTACCTAA	LNDKDKTYAVLHIPYNWTPT*	-1.802	0.000383	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23625	MGLSPYNCTFVNPSHDMLQI			20	SLAY-screened peptide P1975			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGGCCTGTCTCCTTACAATTGTACCTTCGTTAACCCTTCTCATGATATGCTTCAGATCTAA	MGLSPYNCTFVNPSHDMLQI*	-1.801	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23626	CRKPVPTHVFTHFGYSIQIS			20	SLAY-screened peptide P1976			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCGTAAGCCTGTTCCCACGCACGTGTTTACTCACTTCGGTTATAGCATCCAGATCTCGTAA	CRKPVPTHVFTHFGYSIQIS*	-1.8	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23627	WTNTPHFYSA			10	SLAY-screened peptide P1977			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGACGAACACCCCCCACTTTTATAGTGCGTAGGAGAGGGTTGATACCACCACGCTGATCTAA	WTNTPHFYSA*ERVDTTTLI*	-1.8	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23628	LDPMPVLDPVLIEAHTAATR			20	SLAY-screened peptide P1978			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGATCCTATGCCTGTTCTTGATCCTGTGCTCATTGAGGCCCATACCGCCGCTACGCGCTAA	LDPMPVLDPVLIEAHTAATR*	-1.799	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23629	HAA			3	SLAY-screened peptide P1979			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCTGCTTAGCTTCACCGTAGTATGCCTTACAACAGTAACCCCTATTTTCCCACCAATTAA	HAA*LHRSMPYNSNPYFPTN*	-1.798	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23630	RINVPRGGFTKAFMFRPPPR			20	SLAY-screened peptide P1980			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGATCAACGTTCCCAGGGGCGGTTTCACCAAGGCGTTTATGTTCCGCCCGCCTCCTCGGTAA	RINVPRGGFTKAFMFRPPPR*	-1.798	0.007336	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23631	CRCTMPNLHLDALYMSFCPA			20	SLAY-screened peptide P1981			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCGGTGCACTATGCCTAATCTCCACTTGGACGCTCTTTACATGAGTTTCTGTCCTGCTTAA	CRCTMPNLHLDALYMSFCPA*	-1.798	0.012708	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23632	TWYQCHLYDPHCFKLAP			17	SLAY-screened peptide P1982			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTGGTACCAGTGCCATCTCTATGACCCTCACTGTTTCAAGTTGGCGCCTTAGAACGATTAA	TWYQCHLYDPHCFKLAP*ND*	-1.797	0.0014	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23633	LKTTIDLSPVVLCGPNLHKT			20	SLAY-screened peptide P1983			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAAGACTACGATCGACCTGTCTCCGGTCGTCCTCTGCGGCCCTAATTTGCATAAGACCTAA	LKTTIDLSPVVLCGPNLHKT*	-1.797	0.001574	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23634	VPSLDTVGCIPVTDNNHSLT			20	SLAY-screened peptide P1984			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCCGAGTCTTGATACTGTGGGGTGTATCCCTGTTACTGATAATAACCATTCCCTTACGTAA	VPSLDTVGCIPVTDNNHSLT*	-1.797	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23635	YSVHSTHHVLEQSSAFNNNI			20	SLAY-screened peptide P1985			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACAGTGTTCACAGTACTCATCACGTCCTTGAGCAGTCTAGTGCGTTTAACAACAACATCTAA	YSVHSTHHVLEQSSAFNNNI*	-1.797	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23636	SAPFLVRLPSMFT			13	SLAY-screened peptide P1986			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGCCCCGTTCTTGGTCAGACTCCCCTCGATGTTTACTTGACGACTTTTCAGAGCCGCTAAC	SAPFLVRLPSMFT*RLFRAAN	-1.797	0.000347	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23637	EG			2	SLAY-screened peptide P1987			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGGTTAGCGTTAGAGTCTCTAGCGCCCTCAGCTCATTTGGAAGCCTTTTTCGAATGACTAA	EG*R*SL*RPQLIWKPFSND*	-1.796	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23638	RNNTFHFPKCLNKSFFHMQY			20	SLAY-screened peptide P1988			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAACAATACTTTTCATTTCCCTAAGTGCCTGAATAAGAGTTTCTTTCACATGCAGTATTAA	RNNTFHFPKCLNKSFFHMQY*	-1.796	0.000681	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23639	FSCSNPNPSYSSLPFTWWYL			20	SLAY-screened peptide P1989			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTCTTGCTCGAACCCTAACCCTTCCTATAGCTCCCTGCCGTTCACGTGGTGGTATCTCTAA	FSCSNPNPSYSSLPFTWWYL*	-1.796	0.00251	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23640	DYTHVFTFHLI			11	SLAY-screened peptide P1990			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTATACGCACGTGTTTACCTTCCATCTTATTTAGTCTACTAACATGTGTATCTGCTGTTAA	DYTHVFTFHLI*STNMCICC*	-1.795	0.003063	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23641	REAFRMVYF			9	SLAY-screened peptide P1991			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGAGGCGTTTCGGATGGTTTACTTCTAGAGGTTCTACGATATCAGAATTCTGTTCCTTAAC	REAFRMVYF*RFYDIRILFLN	-1.795	0.031861	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23642	PP			2	SLAY-screened peptide P1992			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCCTAGTAGGACTCCTGTTCTGCTTATCATTTTTCGTCTCTCAACATTGATTTTTACTAA	PP**DSCSAYHFSSLNIDFY*	-1.795	0.010814	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23643	HDQCPSLTR			9	SLAY-screened peptide P1993			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGACCAGTGTCCTAGCCTTACTCGGTAGGCGTGTCCTGCCTCTTTTCAGTCTAATTACTAA	HDQCPSLTR*ACPASFQSNY*	-1.794	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23644	HYAPPCPRSPFNDYIIVHAI			20	SLAY-screened peptide P1994			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTATGCGCCCCCCTGCCCCAGGAGCCCTTTTAACGATTATATCATCGTTCACGCGATCTAA	HYAPPCPRSPFNDYIIVHAI*	-1.794	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23645	KGAASSKSTREELAFLHTDI			20	SLAY-screened peptide P1995			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGGGGGCCGCTTCCTCGAAGTCCACGCGGGAGGAGCTGGCTTTCCTTCATACGGATATCTAA	KGAASSKSTREELAFLHTDI*	-1.794	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23646	SPQESRWYRLQTFII			15	SLAY-screened peptide P1996			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCCTCAGGAGTCTCGCTGGTACCGTCTTCAGACTTTCATCATTTGACCGCCCATAGTTAAC	SPQESRWYRLQTFII*PPIVN	-1.792	0.009361	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23647	MIAPTTISPPSRASYHNFCG			20	SLAY-screened peptide P1997			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGATTGCCCCTACCACTATTTCGCCTCCTTCCCGCGCCTCGTACCATAACTTCTGCGGTTAA	MIAPTTISPPSRASYHNFCG*	-1.792	3.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23648	TLFSIACENHAVACRFRRAS			20	SLAY-screened peptide P1998			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCTGTTTTCCATTGCTTGCGAGAATCATGCCGTCGCTTGCAGGTTTCGTCGCGCGTCTTAA	TLFSIACENHAVACRFRRAS*	-1.792	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23649	DPSSFYTRSAIGCTWCFYYC			20	SLAY-screened peptide P1999			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCCCTCGTCTTTTTACACTCGTAGTGCTATTGGCTGCACCTGGTGCTTTTATTACTGCTAA	DPSSFYTRSAIGCTWCFYYC*	-1.792	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23650	CPFTTLSLIVCLRLSRWSTCR			21	SLAY-screened peptide P2000			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCGTTTACGACTCTCTCATTAATCGTCTGCTTAAGACTCTCGAGATGGTCGACCTGCAGG	CPFTTLSLIVCLRLSRWSTCR	-1.792	0.012705	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23651	LHNPHHHANGNYNYDYSTWK			20	SLAY-screened peptide P2001			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCACAATCCGCATCACCACGCCAACGGTAACTATAATTACGACTACTCTACCTGGAAGTAA	LHNPHHHANGNYNYDYSTWK*	-1.791	0.000992	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23652	PLQPNCLFFCLGGSVYYQDHY			21	SLAY-screened peptide P2002			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTCCAGCCGAACTGCTTGTTCTTCTGTCTGGGCGGCTCTGTCTATTACCAGGACCATTAC	PLQPNCLFFCLGGSVYYQDHY	-1.791	3.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23653	IMLHLATFTSQNQGSYGASL			20	SLAY-screened peptide P2003			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCATGCTCCATCTGGCCACCTTCACTAGTCAGAATCAGGGTAGTTACGGGGCGTCTCTTTAA	IMLHLATFTSQNQGSYGASL*	-1.791	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23654	FNFILMPWHLIICLRTRISI			20	SLAY-screened peptide P2004			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAACTTCATTCTTATGCCCTGGCATCTCATCATTTGTCTGCGTACTCGGATTTCTATTTAA	FNFILMPWHLIICLRTRISI*	-1.791	0.022296	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23655	IVMADTPNFTTRYIKYTVKD			20	SLAY-screened peptide P2005			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGTGATGGCGGACACCCCGAACTTTACCACGCGGTACATTAAGTACACTGTTAAGGACTAA	IVMADTPNFTTRYIKYTVKD*	-1.79	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23656	PYPLQPTNYNSETEPKDDNA			20	SLAY-screened peptide P2006			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTATCCTCTGCAGCCCACTAACTACAATAGTGAGACGGAGCCTAAGGACGACAATGCGTAA	PYPLQPTNYNSETEPKDDNA*	-1.79	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23657	CLYIRMHHPTHSHCATAYNL			20	SLAY-screened peptide P2007			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTCTACATTAGGATGCATCACCCCACTCATAGCCATTGTGCGACGGCTTATAACCTCTAA	CLYIRMHHPTHSHCATAYNL*	-1.79	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23658	PCSPFAPYRFPMLRWARVSA			20	SLAY-screened peptide P2008			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGTAGTCCGTTCGCCCCTTATCGGTTTCCCATGCTGAGGTGGGCTCGTGTGAGCGCCTAA	PCSPFAPYRFPMLRWARVSA*	-1.79	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23659	PSTGCINNENFSTPMPVYII			20	SLAY-screened peptide P2009			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGTACCGGTTGTATCAACAACGAGAACTTTTCCACTCCTATGCCTGTGTATATTATTTAA	PSTGCINNENFSTPMPVYII*	-1.79	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23660	SSSAALFQPPYSLPDSELAN			20	SLAY-screened peptide P2010			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTCTTCCGCGGCGCTCTTCCAGCCCCCTTATAGCCTTCCTGACAGTGAGCTTGCGAACTAA	SSSAALFQPPYSLPDSELAN*	-1.789	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23661	PV			2	SLAY-screened peptide P2011			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGTCTAGCCCCAGTGGCTTCCGCGTCTCCCTCTTATTAACTACCTGTTCGCCCTGCGCTAA	PV*PQWLPRLPLINYLFALR*	-1.789	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23662	SAGRFIPGTPIMNVMYPCVV			20	SLAY-screened peptide P2012			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGCTGGGCGCTTTATTCCCGGGACCCCTATCATGAATGTCATGTATCCTTGCGTTGTTTAA	SAGRFIPGTPIMNVMYPCVV*	-1.789	4.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23663	INIILRLILNFFYVFWLANS			20	SLAY-screened peptide P2013			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAACATTATTCTTCGCCTGATCCTGAATTTCTTCTACGTCTTCTGGCTTGCTAATTCTTAA	INIILRLILNFFYVFWLANS*	-1.789	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23664	DACSYFITYRSITKDTCTCY			20	SLAY-screened peptide P2014			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGCTTGCTCGTACTTCATCACTTACCGTAGTATTACGAAGGATACCTGTACTTGTTATTAA	DACSYFITYRSITKDTCTCY*	-1.788	0.003591	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23665	TLDFTKPVISVNARSPRTQK			20	SLAY-screened peptide P2015			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTCGATTTCACCAAGCCTGTTATCTCGGTCAATGCGAGGTCTCCCCGCACCCAGAAGTAA	TLDFTKPVISVNARSPRTQK*	-1.788	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23666	LTDYNSNFIPNSALLLHTHR			20	SLAY-screened peptide P2016			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCACCGACTACAATTCGAACTTTATCCCGAATAGCGCTCTTCTTTTGCATACTCACCGGTAA	LTDYNSNFIPNSALLLHTHR*	-1.788	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23667	PAISRCFAKRYYATHESTVD			20	SLAY-screened peptide P2017			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCTATCTCTCGCTGTTTTGCCAAGCGCTACTATGCCACCCATGAGTCTACTGTCGACTAA	PAISRCFAKRYYATHESTVD*	-1.788	0.001598	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23668	RCSPAGADPRHHTSIYDDDG			20	SLAY-screened peptide P2018			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTGTAGCCCTGCCGGGGCTGACCCGCGTCACCATACGTCTATTTACGATGACGACGGTTAA	RCSPAGADPRHHTSIYDDDG*	-1.787	0.002126	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23669	HCHHVINAKNSSMSSSVDYI			20	SLAY-screened peptide P2019			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGCCACCATGTTATTAATGCCAAGAACAGTAGCATGTCCTCTTCCGTTGACTATATTTAA	HCHHVINAKNSSMSSSVDYI*	-1.787	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23670	NFRACQPTSNDSDLVN			16	SLAY-screened peptide P2020			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTTTCGGGCGTGTCAGCCTACTTCCAATGACTCGGATCTGGTCAATTAGATTATTTTCTAA	NFRACQPTSNDSDLVN*IIF*	-1.787	0.000128	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23671	HQAFQHTGSPHTVHPLSHRH			20	SLAY-screened peptide P2021			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCAGGCGTTCCAGCACACTGGCTCGCCGCACACGGTGCACCCCCTTAGTCATAGGCACTAA	HQAFQHTGSPHTVHPLSHRH*	-1.786	0.000548	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23672	QITYHTAPIRVITNFQVFDG			20	SLAY-screened peptide P2022			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGATTACTTACCACACGGCGCCTATCAGGGTCATTACGAATTTTCAGGTTTTTGACGGTTAA	QITYHTAPIRVITNFQVFDG*	-1.786	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23673	PRM			3	SLAY-screened peptide P2023			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGTATGTAGCGCATCCCCGGTCCTAACGTCCTTTGCTTGTGCGGTGCCCCCCTCTTGTAA	PRM*RIPGPNVLCLCGAPLL*	-1.786	0.004325	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23674	VARAHNTLCTSHRLLCFNFY			20	SLAY-screened peptide P2024			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGCCCGTGCGCATAATACCCTTTGTACCAGTCATCGTCTTCTTTGCTTCAATTTTTATTAA	VARAHNTLCTSHRLLCFNFY*	-1.785	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23675	QHTRSKLFCTLSPNRRIAIY			20	SLAY-screened peptide P2025			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCATACGCGCTCTAAGCTTTTCTGCACTCTTTCTCCTAACCGTCGGATCGCCATCTATTAA	QHTRSKLFCTLSPNRRIAIY*	-1.785	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23676	PHLQSWGSRHPPLVSCDNTS			20	SLAY-screened peptide P2026			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCACTTGCAGAGTTGGGGGTCCCGCCATCCTCCCCTCGTCTCCTGTGACAATACTTCTTAA	PHLQSWGSRHPPLVSCDNTS*	-1.785	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23677	LSIIICMIYLPFKMSVIQTD			20	SLAY-screened peptide P2027			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGAGCATTATCATCTGTATGATCTATCTCCCCTTTAAGATGTCTGTCATTCAGACTGATTAA	LSIIICMIYLPFKMSVIQTD*	-1.785	8.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23678	GPTPRCTSHSWTSPPMIFII			20	SLAY-screened peptide P2028			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCCCACCCCGCGCTGTACCTCGCACAGCTGGACTTCTCCGCCGATGATTTTCATTATTTAA	GPTPRCTSHSWTSPPMIFII*	-1.785	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23679	ARIT			4	SLAY-screened peptide P2029			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCGGATCACTTAGAGTTGGATCCTGCCGACTACCACTTCCCAGCATACTCGTGTTTTTTAA	ARIT*SWILPTTTSQHTRVF*	-1.785	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23680	PAPVLGSLIRTTPPGAPCFH			20	SLAY-screened peptide P2030			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGCTCCTGTCTTGGGGAGTCTCATTCGGACTACTCCTCCTGGTGCCCCGTGTTTCCATTAA	PAPVLGSLIRTTPPGAPCFH*	-1.784	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23681	SLTYQYATPVSNNMSYADLD			20	SLAY-screened peptide P2031			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCTTACCTACCAGTATGCGACTCCTGTTAGCAATAATATGTCGTACGCTGATCTTGATTAA	SLTYQYATPVSNNMSYADLD*	-1.784	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23682	PLSRSPTRSAHRYGNRDSNA			20	SLAY-screened peptide P2032			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTTTCCCGTAGCCCGACGCGTAGTGCGCATCGCTACGGCAATCGGGACTCTAATGCTTAA	PLSRSPTRSAHRYGNRDSNA*	-1.784	0.000172	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23683	SASHSTGYLYYPKSPCTNAN			20	SLAY-screened peptide P2033			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCGTCTCATTCCACCGGTTACCTCTATTATCCTAAGTCTCCTTGCACCAACGCTAACTAA	SASHSTGYLYYPKSPCTNAN*	-1.784	0.002517	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23684	CAALFPRPHHVPPDPLDPDT			20	SLAY-screened peptide P2034			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCTGCCTTGTTTCCGCGTCCGCATCATGTTCCGCCTGATCCCCTCGATCCCGATACGTAA	CAALFPRPHHVPPDPLDPDT*	-1.784	0.002706	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23685	APPFGHPYICSKSG			14	SLAY-screened peptide P2035			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCCGCCCTTTGGCCACCCCTATATTTGCTCTAAGTCTGGGTAGCGGTTTACTCGTGATTAA	APPFGHPYICSKSG*RFTRD*	-1.784	0.007807	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23686	YYTGPTTHNWHFALESDSRD			20	SLAY-screened peptide P2036			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTATACTGGGCCGACCACCCATAACTGGCACTTCGCCCTGGAGTCTGATTCTAGGGACTAA	YYTGPTTHNWHFALESDSRD*	-1.783	0.01191	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23687	PLDYHTARRARCYHSFGHYH			20	SLAY-screened peptide P2037			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTCGACTATCATACTGCCCGCCGCGCTCGTTGTTACCACAGTTTTGGGCATTATCATTAA	PLDYHTARRARCYHSFGHYH*	-1.783	0.001239	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23688	ICFQRLYNQALYHATLRTRH			20	SLAY-screened peptide P2038			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTGCTTCCAGCGCCTCTATAACCAGGCTCTCTATCACGCTACTCTCAGGACTCGGCACTAA	ICFQRLYNQALYHATLRTRH*	-1.782	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23689	REEHTHYACSFINNVPNRWY			20	SLAY-screened peptide P2039			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGAGGAGCACACCCATTATGCTTGCTCGTTTATTAATAATGTTCCCAACCGCTGGTACTAA	REEHTHYACSFINNVPNRWY*	-1.782	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23690	KTQQNFGI			8	SLAY-screened peptide P2040			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGACGCAGCAGAACTTTGGGATTTAGAAGAAGAACCATCGCAATCCGTTGAAGTTGCTTTAA	KTQQNFGI*KKNHRNPLKLL*	-1.782	0.004999	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23691	NSCPLAPIVRATYTWSIVVC			20	SLAY-screened peptide P2041			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTCGTGCCCCTTGGCCCCTATTGTTCGTGCCACCTACACTTGGTCGATCGTCGTTTGTTAA	NSCPLAPIVRATYTWSIVVC*	-1.781	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23692	VITTFSNKTSSGHRPTCTAR			20	SLAY-screened peptide P2042			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTATTACCACCTTTTCGAACAAGACGTCGTCTGGCCACAGGCCCACGTGCACTGCTCGTTAA	VITTFSNKTSSGHRPTCTAR*	-1.781	0.004203	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23693	SDSTSCDLVFEYSLI			15	SLAY-screened peptide P2043			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGACTCTACCTCCTGTGACCTGGTCTTTGAGTACAGTCTTATCTAGTTCCCTTGGGTGTAA	SDSTSCDLVFEYSLI*FPWV*	-1.78	0.020452	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23694	YQFHLHPPNVRPSIVYTVRY			20	SLAY-screened peptide P2044			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCAGTTTCATCTTCATCCTCCGAATGTTCGCCCGTCTATCGTCTATACCGTGCGGTACTAA	YQFHLHPPNVRPSIVYTVRY*	-1.78	0.008137	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23695	SPQQANNAIRAYFNALYDCN			20	SLAY-screened peptide P2045			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCCTCAGCAGGCGAATAATGCCATCCGGGCTTACTTTAATGCTCTCTATGACTGCAATTAA	SPQQANNAIRAYFNALYDCN*	-1.78	0.004334	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23696	RNMMFPDCLGGNPKVSPILFN			21	SLAY-screened peptide P2046			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAATATGATGTTTCCGGATTGCCTTGGCGGTAACCCCAAGGTCTCTCCAATACTTTTTAAC	RNMMFPDCLGGNPKVSPILFN	-1.78	0.005228	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23697	FPAPNYV			7	SLAY-screened peptide P2047			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCCCGCGCCTAATTACGTGTAGGGCCGTTGGCAGTTCTACTCGCAGACCCCTAAGATCTAA	FPAPNYV*GRWQFYSQTPKI*	-1.779	0.012605	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23698	FSVNTTLAQAEVIGYHDI			18	SLAY-screened peptide P2048			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTCCGTCAATACTACCCTTGCGCAGGCGGAGGTGATTGGCTACCACGACATTTAGCATTAA	FSVNTTLAQAEVIGYHDI*H*	-1.779	0.011524	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23699	TAHHFLIPLVPTKMILFSRH			20	SLAY-screened peptide P2049			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGCCCATCATTTCCTTATTCCTCTGGTTCCTACTAAGATGATCCTGTTCTCGCGTCACTAA	TAHHFLIPLVPTKMILFSRH*	-1.779	4.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23700	PLLTRATISLHTHTPQDANR			20	SLAY-screened peptide P2050			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTCTTGACGCGCGCCACGATTTCTCTGCACACGCACACGCCCCAGGACGCTAACCGCTAA	PLLTRATISLHTHTPQDANR*	-1.779	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23701	AFTCYITHYSTPSNQRTMNT			20	SLAY-screened peptide P2051			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTTTACTTGTTACATTACCCATTATTCTACCCCGTCGAATCAGCGTACTATGAACACCTAA	AFTCYITHYSTPSNQRTMNT*	-1.778	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23702	QVSFNPSSTTYTD			13	SLAY-screened peptide P2052			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGTTAGCTTTAATCCTTCGTCGACTACGTATACTGATTAGTGGTTCATGGGTAATGACTAA	QVSFNPSSTTYTD*WFMGND*	-1.778	0.000625	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23703	DIQIDRTHHNFKITLTLTCCN			21	SLAY-screened peptide P2053			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATATTCAGATTGATCGCACGCACCATAATTTCAAAATCACCCTAACCCTCACGTGTTGTAAC	DIQIDRTHHNFKITLTLTCCN	-1.778	0.009228	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23704	PRLDTCT			7	SLAY-screened peptide P2054			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGGTTGGACACTTGTACTTAGATTCCCGCTAATGCTGGGACGGTGTCGGATCATGTCTAA	PRLDTCT*IPANAGTVSDHV*	-1.778	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23705	HYRCHHSHRAPGLGTIIILIN			21	SLAY-screened peptide P2055			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTACCGTTGTCACCACAGCCACCGGGCTCCCGGTTTGGGAACAATTATAATTCTTATTAAC	HYRCHHSHRAPGLGTIIILIN	-1.778	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23706	EDLEMVQRGLRQDRERPLS			19	SLAY-screened peptide P2056			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGACCTCGAGATGGTGCAGCGGGGCCTGCGGCAGGATCGGGAGCGGCCCCTCAGCTAGTAA	EDLEMVQRGLRQDRERPLS**	-1.778	0.006193	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23707	HCLPLP			6	SLAY-screened peptide P2057			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTGCCTGCCCTTGCCCTAGCCGAACAGGCCTATTACCACCGTGACGCTTTGTCGGCCTTAA	HCLPLP*PNRPITTVTLCRP*	-1.778	0.000392	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23708	GKHSTVLLFRPHFHSDCIGA			20	SLAY-screened peptide P2058			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGAAGCACTCGACTGTTCTCCTGTTTCGCCCCCATTTTCATTCGGATTGTATCGGCGCTTAA	GKHSTVLLFRPHFHSDCIGA*	-1.778	0.001216	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23709	RNTVRPAIKALHVRKNQ			17	SLAY-screened peptide P2059			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGAACACCGTGCGCCCCGCCATTAAGGCTCTGCACGTTCGCAAGAACCAGTAGAACCTCTAA	RNTVRPAIKALHVRKNQ*NL*	-1.778	0.029668	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23710	SHNAPTQRNVAIATTRLRVA			20	SLAY-screened peptide P2060			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCACAACGCCCCTACTCAGCGCAACGTCGCCATCGCGACGACTAGGCTCCGTGTTGCGTAA	SHNAPTQRNVAIATTRLRVA*	-1.777	0.016337	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23711	DVNSYRPNDHPYFPMQILSF			20	SLAY-screened peptide P2061			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGTTAATTCTTATCGTCCGAATGATCACCCCTACTTTCCTATGCAGATTCTCTCGTTCTAA	DVNSYRPNDHPYFPMQILSF*	-1.777	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23712	YFSQYFFKPCGDPSEYAKIV			20	SLAY-screened peptide P2062			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTTTTCGCAGTATTTCTTTAAGCCCTGCGGCGACCCGTCGGAGTATGCCAAGATCGTCTAA	YFSQYFFKPCGDPSEYAKIV*	-1.777	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23713	RAVNDISRRQ			10	SLAY-screened peptide P2063			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCCGTGAACGACATCTCTCGGCGCCAGTAGGGTACTCTCAGTCAGGGAGCTACCTTTAAC	RAVNDISRRQ*GTLSQGATFN	-1.777	0.000888	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23714	RTPLSSTGATALANVAY			17	SLAY-screened peptide P2064			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTACCCCCCTTAGCTCTACCGGGGCGACGGCTCTTGCTAACGTTGCCTATTAGCCTATTTAA	RTPLSSTGATALANVAY*PI*	-1.777	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23715	HKTTYDPDLVVCLSHSTSPH			20	SLAY-screened peptide P2065			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAAGACGACGTACGATCCCGATCTCGTGGTTTGCCTGTCCCATTCGACTTCCCCCCACTAA	HKTTYDPDLVVCLSHSTSPH*	-1.777	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23716	LTTVTQSALPTTSVLPILQNY			21	SLAY-screened peptide P2066			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCACTACTGTCACTCAGTCTGCTCTGCCTACCACCTCGGTTTTGCCCATTCTGCAGAATTAT	LTTVTQSALPTTSVLPILQNY	-1.776	0.014123	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23717	PIRLIAYDLLGYVMGN			16	SLAY-screened peptide P2067			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATCAGGCTCATCGCCTATGACCTCCTTGGGTACGTCATGGGTAATTAGAACACCCCTTAA	PIRLIAYDLLGYVMGN*NTP*	-1.776	0.012248	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23718	PPSDHTWTAHHYHLSPGHPH			20	SLAY-screened peptide P2068			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCTAGCGATCATACTTGGACCGCCCACCATTATCACTTGAGCCCTGGGCACCCTCATTAA	PPSDHTWTAHHYHLSPGHPH*	-1.775	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23719	PDTVETAPWAGSPNQCRTTS			20	SLAY-screened peptide P2069			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGATACGGTTGAGACCGCGCCTTGGGCTGGGAGCCCGAATCAGTGTCGTACTACGTCCTAA	PDTVETAPWAGSPNQCRTTS*	-1.775	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23720	FAFLTWPYYVLNLVVHNKDS			20	SLAY-screened peptide P2070			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGCGTTTCTGACTTGGCCTTACTACGTTCTGAACCTCGTTGTCCATAACAAGGATTCCTAA	FAFLTWPYYVLNLVVHNKDS*	-1.775	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23721	KSLQTCQRRHCRIHVWYTNS			20	SLAY-screened peptide P2071			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGTCGTTGCAGACCTGTCAGAGGCGCCACTGCAGGATTCACGTGTGGTATACGAATAGTTAA	KSLQTCQRRHCRIHVWYTNS*	-1.775	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23722	LLT			3	SLAY-screened peptide P2072			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCTTACTTAGGTCTGCCCGCTTAATTCCCTCCCTACTATCTCTATTTACTGCTGTTGTTAA	LLT*VCPLNSLPTISIYCCC*	-1.775	0.014628	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23723	LERVPCFICTTTSLTYYIRM			20	SLAY-screened peptide P2073			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGAGCGGGTGCCCTGTTTTATCTGCACTACTACCTCTCTCACTTATTATATCAGGATGTAA	LERVPCFICTTTSLTYYIRM*	-1.775	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23724	TTMNRNTFTDYTCRSVPLFI			20	SLAY-screened peptide P2074			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGACTATGAACCGTAATACTTTCACCGATTATACTTGCAGGTCGGTTCCCTTGTTTATCTAA	TTMNRNTFTDYTCRSVPLFI*	-1.775	0.017721	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23725	NCNSFPFYGLLYHNVDASLT			20	SLAY-screened peptide P2075			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTGTAATTCCTTTCCTTTCTACGGCCTGCTCTATCACAACGTTGATGCTAGCCTCACGTAA	NCNSFPFYGLLYHNVDASLT*	-1.774	0.000399	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23726	IVVAVVEISNHRDVQSSEKL			20	SLAY-screened peptide P2076			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGTTGTCGCTGTTGTGGAGATTAGCAATCACAGGGATGTCCAGAGTTCTGAGAAGTTGTAA	IVVAVVEISNHRDVQSSEKL*	-1.774	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23727	IMQHCRTQLCIIMYTTLTSPN			21	SLAY-screened peptide P2077			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCATGCAGCACTGCCGGACCCAGCTGTGCATTATTATGTATACTACACTTACGTCACCTAAC	IMQHCRTQLCIIMYTTLTSPN	-1.773	0.000394	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23728	VHVDRLPRTLSCTRSIT			17	SLAY-screened peptide P2078			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCATGTTGACCGTCTTCCTCGCACTCTAAGCTGCACGCGTAGTATTACTTGACCCCTTAAC	VHVDRLPRTLSCTRSIT*PLN	-1.773	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23729	PTPWCFLHRTSRPKRRTPAA			20	SLAY-screened peptide P2079			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACCCCTTGGTGCTTCCTTCATCGCACCTCCCGTCCTAAGAGGCGCACTCCCGCTGCCTAA	PTPWCFLHRTSRPKRRTPAA*	-1.773	0.003652	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23730	PCFDNRDNILLDYWFHSAII			20	SLAY-screened peptide P2080			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGCTTTGACAATCGGGATAATATTTTGCTTGATTATTGGTTCCATAGCGCTATTATTTAA	PCFDNRDNILLDYWFHSAII*	-1.773	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23731	GLSTLPPSVRSQRLSFTLNH			20	SLAY-screened peptide P2081			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCTTTCCACCCTCCCCCCTTCCGTTCGGAGCCAGCGTCTGTCTTTCACCCTGAACCATTAA	GLSTLPPSVRSQRLSFTLNH*	-1.773	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23732	RHMHSLTSHAGGRKPARAYY			20	SLAY-screened peptide P2082			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCACATGCATTCCCTTACGTCCCATGCTGGTGGCCGCAAGCCTGCTCGGGCTTATTATTAA	RHMHSLTSHAGGRKPARAYY*	-1.772	0.004599	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23733	TLPWQLPCLHWA			12	SLAY-screened peptide P2083			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTTCCTTGGCAGCTGCCGTGCCTGCACTGGGCGTAGGTTCAGTGCGACTTTTGGAGTTAA	TLPWQLPCLHWA*VQCDFWS*	-1.772	0.000781	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23734	PQALVVRLRA			10	SLAY-screened peptide P2084			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCAGGCCCTGGTGGTACGATTAAGGGCCTGACCAGTTTTATATCAAGAGTGATTATTAACT	PQALVVRLRA*PVLYQE*LLT	-1.772	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23735	VEPVMREVPLICTRVATA			18	SLAY-screened peptide P2085			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGAGCCGGTGATGCGTGAGGTTCCCCTCATCTGTACGAGAGTTGCAACAGCATGAACTAAC	VEPVMREVPLICTRVATA*TN	-1.772	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23736	VLVPGRGVRCRICPSSMGAR			20	SLAY-screened peptide P2086			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCTGGTCCCCGGGCGTGGTGTTAGGTGTCGGATTTGTCCTAGTAGCATGGGCGCTCGGTAA	VLVPGRGVRCRICPSSMGAR*	-1.771	0.000241	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23737	WLLTSSHINNTFTAYTTTRC			20	SLAY-screened peptide P2087			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCTCCTGACGTCCTCCCACATTAATAACACCTTTACCGCTTACACTACTACGAGGTGCTAA	WLLTSSHINNTFTAYTTTRC*	-1.771	0.001836	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23738	TNSILSHLSNCRNLFWYDIY			20	SLAY-screened peptide P2088			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGAACTCGATCCTCTCCCATTTGTCCAACTGTCGCAATCTGTTCTGGTATGACATCTACTAA	TNSILSHLSNCRNLFWYDIY*	-1.771	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23739	PPGRCGCMPDRVNNRMMPPS			20	SLAY-screened peptide P2089			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCTGGCCGTTGCGGGTGCATGCCTGATCGTGTGAACAATCGCATGATGCCTCCGTCGTAA	PPGRCGCMPDRVNNRMMPPS*	-1.77	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23740	LKPMHPQAGRLLFTKGFTDA			20	SLAY-screened peptide P2090			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAAGCCCATGCACCCTCAGGCCGGCCGGCTTCTCTTCACTAAGGGCTTTACCGACGCCTAA	LKPMHPQAGRLLFTKGFTDA*	-1.77	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23741	CWHSQHRCFHYGCPPYDFLV			20	SLAY-screened peptide P2091			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTGGCACAGTCAGCACCGTTGTTTTCACTATGGTTGTCCTCCTTACGACTTTCTCGTGTAA	CWHSQHRCFHYGCPPYDFLV*	-1.769	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23742	PPPSRSVGPILALCRSSAN			19	SLAY-screened peptide P2092			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCCCCTCGAGATCGGTAGGGCCCATATTAGCTCTGTGCCGGAGTTCCGCTAACTGAGTA	PPPSRSVGPILALCRSSAN*V	-1.769	0.025598	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23743	RLSDNTHTLHRCTARIK			17	SLAY-screened peptide P2093			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTTGTCTGATAATACCCACACGCTTCACAGGTGTACCGCTAGGATCAAGTAGTGCCATTAA	RLSDNTHTLHRCTARIK*CH*	-1.769	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23744	DFDAVCSPLSSWACPV			16	SLAY-screened peptide P2094			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTTCGACGCCGTCTGTTCTCCTTTGAGCAGCTGGGCTTGCCCGGTCTAGATCACCTACTAA	DFDAVCSPLSSWACPV*ITY*	-1.768	0.021849	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23745	VSSCWTRGSAGRTSTIFSLF			20	SLAY-screened peptide P2095			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTCTAGTTGTTGGACTCGTGGCAGCGCTGGCCGTACGTCTACGATCTTCTCCCTCTTTTAA	VSSCWTRGSAGRTSTIFSLF*	-1.768	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23746	EDINPIGPSCYPVRSSRYIV			20	SLAY-screened peptide P2096			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGACATTAATCCCATCGGTCCGTCTTGCTATCCTGTGCGCAGTTCTCGGTACATCGTGTAA	EDINPIGPSCYPVRSSRYIV*	-1.768	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23747	HSPSCLSKSAGRTNNSPEPL			20	SLAY-screened peptide P2097			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTCCCCCTCGTGTCTCAGTAAGTCCGCTGGCCGCACGAATAATAGCCCCGAGCCGCTCTAA	HSPSCLSKSAGRTNNSPEPL*	-1.767	0.002451	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23748	YRSVLIYLL			9	SLAY-screened peptide P2098			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCGGTCCGTCTTGATCTATCTGTTGTAGTAGTTCATCTAGATCAACAGTCATGTTAGGTAA	YRSVLIYLL**FI*INSHVR*	-1.767	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23749	CIGGPPTRRCYCQFCHYLPLT			21	SLAY-screened peptide P2099			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTATCGGTGGTCCTCCGACCCGTCGGTGTTATTGTCAATTTTGCCATTATTTACCGCTAACT	CIGGPPTRRCYCQFCHYLPLT	-1.767	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23750	LFPPRPRAYMHFPYFA			16	SLAY-screened peptide P2100			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTTCCCCCCGCGGCCTCGGGCGTATATGCATTTTCCTTATTTCGCTTAGGACGACCGTTAA	LFPPRPRAYMHFPYFA*DDR*	-1.767	0.0016	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23751	PHTIRRLARPRAYHLCSVHS			20	SLAY-screened peptide P2101			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCACACGATTAGGCGTCTTGCTCGGCCTCGCGCGTACCACCTCTGTTCTGTTCACTCTTAA	PHTIRRLARPRAYHLCSVHS*	-1.766	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23752	DSSL			4	SLAY-screened peptide P2102			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTCCTCGCTGTAGATTTTGCTGCGCGACGACCATGATCCCAATGATCCCTACTGGAATTAA	DSSL*ILLRDDHDPNDPYWN*	-1.766	0.008096	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23753	WMPRSGLT			8	SLAY-screened peptide P2103			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGATGCCCAGGTCTGGCCTTACCTAGAACAGTGATAACCCTTAGACGTTCTATTCCCCTTAA	WMPRSGLT*NSDNP*TFYSP*	-1.765	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23754	MPTHPIPCITI			11	SLAY-screened peptide P2104			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCCTACTCATCCGATCCCTTGTATTACGATTTAGGACTCTTAGTCCCCCCGGTAGACTTAA	MPTHPIPCITI*DS*SPR*T*	-1.765	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23755	MHAGDCS			7	SLAY-screened peptide P2105			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCACGCCGGCGATTGCTCTTAGTAGTCTAATCGGAATTACTCTACGATTTTCGTCGACTAA	MHAGDCS**SNRNYSTIFVD*	-1.765	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23756	QIFLASNDTTPMIANNVLPC			20	SLAY-screened peptide P2106			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGATTTTCTTGGCGAGTAATGACACTACGCCGATGATTGCTAACAACGTTCTTCCCTGTTAA	QIFLASNDTTPMIANNVLPC*	-1.765	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23757	EKRARRVNA			9	SLAY-screened peptide P2107			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGAAGCGCGCTAGGCGTGTCAACGCTTAGATCGGCCTTAGTATCACTTCCGACACTCCGTAA	EKRARRVNA*IGLSITSDTP*	-1.764	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23758	PDTCRDRRWPSHSLLIASRL			20	SLAY-screened peptide P2108			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGACACTTGCCGTGATCGTCGTTGGCCCTCTCACAGTCTGCTTATCGCTAGCCGGCTTTAA	PDTCRDRRWPSHSLLIASRL*	-1.764	0.01722	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23759	SLTEGPRRRQTSLSVSVQGH			20	SLAY-screened peptide P2109			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTTGACGGAGGGTCCTAGGCGCCGGCAGACTTCCCTCTCCGTGTCTGTCCAGGGTCACTAA	SLTEGPRRRQTSLSVSVQGH*	-1.764	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23760	HYSLSLIRTTANNGCPVVHS			20	SLAY-screened peptide P2110			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTACTCCTTGTCTTTGATCAGGACGACTGCTAACAACGGTTGCCCCGTTGTCCACAGCTAA	HYSLSLIRTTANNGCPVVHS*	-1.764	0.031754	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23761	WLCRNILWICKDHTARLHSM			20	SLAY-screened peptide P2111			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCTGTGTCGTAATATCCTGTGGATTTGCAAGGACCATACTGCCAGGCTTCATTCGATGTAA	WLCRNILWICKDHTARLHSM*	-1.763	0.017523	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23762	LFGPCFSRPQTCHHRTLRVT			20	SLAY-screened peptide P2112			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTTGGTCCTTGTTTCAGTCGCCCCCAGACCTGTCACCACCGTACGCTTCGCGTCACTTAA	LFGPCFSRPQTCHHRTLRVT*	-1.763	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23763	SHTLSTHYSSPRLSLVSVGS			20	SLAY-screened peptide P2113			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCATACGCTTAGCACTCACTATAGTTCTCCGCGGCTCAGCCTTGTTTCCGTTGGCAGCTAA	SHTLSTHYSSPRLSLVSVGS*	-1.763	0.019599	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23764	LTICLNTHMGPRVNRLNVSS			20	SLAY-screened peptide P2114			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACTATCTGTCTGAACACGCATATGGGCCCCAGGGTGAATCGTCTCAATGTGTCGAGCTAA	LTICLNTHMGPRVNRLNVSS*	-1.763	0.000441	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23765	WPVLDIFPEQGISSPDTPNP			20	SLAY-screened peptide P2115			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCCTGTCCTGGATATTTTTCCCGAGCAGGGTATTTCCTCGCCTGACACCCCCAACCCGTAA	WPVLDIFPEQGISSPDTPNP*	-1.762	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23766	YHVMSPMRITLCPTQSPAPN			20	SLAY-screened peptide P2116			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCACGTCATGTCCCCTATGCGCATCACCCTGTGCCCTACCCAGAGCCCTGCCCCGAATTAA	YHVMSPMRITLCPTQSPAPN*	-1.762	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23767	SNTKHTSF			8	SLAY-screened peptide P2117			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGAACACGAAGCATACCTCGTTCTAGCCCAGCAATCCCTGGTCTTGTATTAACACGTGGTAA	SNTKHTSF*PSNPWSCINTW*	-1.761	0.000282	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23768	QPRGPPAPGNHTIGPTHLPL			20	SLAY-screened peptide P2118			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCGCGGGGCCCGCCGGCCCCTGGTAACCATACGATCGGTCCCACCCACCTTCCCCTGTAA	QPRGPPAPGNHTIGPTHLPL*	-1.761	0.003555	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23769	APSHATNTTKCTLRPEGWHT			20	SLAY-screened peptide P2119			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCCGTCTCACGCGACGAATACCACCAAGTGCACGCTGCGGCCCGAGGGTTGGCACACCTAA	APSHATNTTKCTLRPEGWHT*	-1.761	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23770	FSGGARPQSSHLLSNLHTCF			20	SLAY-screened peptide P2120			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTCCGGTGGTGCTCGCCCTCAGTCTAGCCATCTCCTGTCGAACCTCCACACTTGTTTCTAA	FSGGARPQSSHLLSNLHTCF*	-1.76	2.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23771	PPRMTNLNEYPVSVHPLRIHL			21	SLAY-screened peptide P2121			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCCGGATGACTAACCTCAACGAGTATCCGGTTAGCGTGCATCCCCTCCGCATCCATTTA	PPRMTNLNEYPVSVHPLRIHL	-1.76	4.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23772	LQSYTALPG			9	SLAY-screened peptide P2122			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCAGTCCTACACTGCCTTGCCGGGGTAGCTCTGCCCCAGTTACCTTAATCGCTGCATCTAA	LQSYTALPG*LCPSYLNRCI*	-1.76	0.037505	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23773	RPCLVIRAALRIRLEFKLRS			20	SLAY-screened peptide P2123			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCGTGTTTGGTTATCCGTGCCGCGCTGCGCATCCGCCTTGAGTTTAAGCTGCGTTCGTAA	RPCLVIRAALRIRLEFKLRS*	-1.76	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23774	TLLSWSSPGGAACNKQTNAN			20	SLAY-screened peptide P2124			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTTCTCAGCTGGAGTTCGCCGGGTGGTGCTGCCTGTAATAAGCAGACTAATGCCAATTAA	TLLSWSSPGGAACNKQTNAN*	-1.759	0.000123	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23775	LNQLNDSASWN			11	SLAY-screened peptide P2125			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAATCAGCTTAATGATAGTGCTTCGTGGAACTAGTGCCATGATGGCTGGGTGCTTTATTAA	LNQLNDSASWN*CHDGWVLY*	-1.759	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23776	IHSRPLSRFHVYTSYCHYGP			20	SLAY-screened peptide P2126			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCACAGCAGGCCGTTGTCGCGGTTTCACGTTTACACTAGTTATTGCCACTATGGTCCCTAA	IHSRPLSRFHVYTSYCHYGP*	-1.759	0.000291	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23777	YFWSALLPNAITSSPLSLSI			20	SLAY-screened peptide P2127			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTTTTGGTCTGCCCTTTTGCCTAACGCTATTACTAGTTCGCCCCTTTCCTTGTCCATCTAA	YFWSALLPNAITSSPLSLSI*	-1.758	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23778	SVSFRRTIVPARDYREVGTF			20	SLAY-screened peptide P2128			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGTGTCGTTCCGTCGCACCATCGTCCCGGCGCGCGACTACCGCGAGGTCGGCACGTTTTAA	SVSFRRTIVPARDYREVGTF*	-1.757	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23779	PTLPHIININLQAFCTLFKY			20	SLAY-screened peptide P2129			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACGCTCCCGCATATTATTAACATTAACCTCCAGGCCTTCTGCACTCTTTTTAAGTATTAA	PTLPHIININLQAFCTLFKY*	-1.757	4.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23780	CLPVSHITYYPGSTLYVGSP			20	SLAY-screened peptide P2130			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTTGCCGGTTTCTCATATCACGTACTACCCCGGCTCCACCCTGTATGTTGGGAGTCCTTAA	CLPVSHITYYPGSTLYVGSP*	-1.756	0.042177	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23781	PVSFNN			6	SLAY-screened peptide P2131			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGTTTCCTTCAACAATTAGCTTCTTGGGACGTTTAACTAGATTCACCCTCCTGTCCTGTAA	PVSFNN*LLGTFN*IHPPVL*	-1.756	0.002337	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23782	LEWFVALYSLTGSFRSRVDH			20	SLAY-screened peptide P2132			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGAGTGGTTCGTGGCCCTGTATTCTTTGACTGGCAGCTTCCGGTCTAGGGTCGACCATTAA	LEWFVALYSLTGSFRSRVDH*	-1.756	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23783	PCLPTIGIAANSTIIATHAS			20	SLAY-screened peptide P2133			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGCCTGCCTACCATCGGTATCGCTGCCAACTCTACCATTATTGCCACCCACGCTTCTTAA	PCLPTIGIAANSTIIATHAS*	-1.755	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23784	ITDPARASSYSTGRPYLFDN			20	SLAY-screened peptide P2134			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCACTGATCCGGCCAGGGCCTCTAGTTACTCGACTGGCCGGCCCTATCTGTTTGATAATTAA	ITDPARASSYSTGRPYLFDN*	-1.755	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23785	DSIMTSAARGVRWVLCLVCVN			21	SLAY-screened peptide P2135			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACAGCATTATGACATCTGCCGCACGAGGAGTACGATGGGTTTTATGCTTAGTCTGCGTTAAC	DSIMTSAARGVRWVLCLVCVN	-1.754	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23786	RCYAYEVILFEACCYLHNHV			20	SLAY-screened peptide P2136			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGCTACGCTTACGAGGTTATCCTTTTTGAGGCTTGTTGTTATCTTCACAACCATGTCTAA	RCYAYEVILFEACCYLHNHV*	-1.753	0.000351	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23787	ALLNCHRSQYAESSSHRQNI			20	SLAY-screened peptide P2137			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCTCCTGAATTGCCACCGTTCCCAGTACGCCGAGTCCTCGTCTCATCGTCAGAACATTTAA	ALLNCHRSQYAESSSHRQNI*	-1.753	0.000129	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23788	PSP			3	SLAY-screened peptide P2138			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGCCCTTAGTTGGCGCTCTCCCGCCGCGACCGCAAGTAGTACAGCAATATCAGTACTTAA	PSP*LALSRRDRK*YSNIST*	-1.753	0.01849	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23789	RTAYPFSDRLKVANFRRAHT			20	SLAY-screened peptide P2139			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACCGCGTACCCCTTCAGCGACCGTCTTAAGGTCGCTAACTTCCGCCGGGCTCACACTTAA	RTAYPFSDRLKVANFRRAHT*	-1.753	0.026433	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23790	SRFLRAPTDNLTCIPL			16	SLAY-screened peptide P2140			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCGCTTTCTTAGGGCGCCCACTGATAATCTTACTTGCATCCCTTTGTAGATGGGCACTTAA	SRFLRAPTDNLTCIPL*MGT*	-1.752	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23791	SPTLVLLILCLRTRCCRHGT			20	SLAY-screened peptide P2141			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCCCACCCTCGTTCTTCTCATTCTCTGCCTTAGGACCCGCTGCTGTCGTCACGGGACTTAA	SPTLVLLILCLRTRCCRHGT*	-1.752	0.000204	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23792	PTTDSLPAGCNFTPLTSQLI			20	SLAY-screened peptide P2142			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACTACCGACAGCCTGCCCGCCGGCTGCAACTTCACCCCGCTGACGAGTCAGCTTATTTAA	PTTDSLPAGCNFTPLTSQLI*	-1.752	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23793	PPFRPRDSPVHMLGCFSCRY			20	SLAY-screened peptide P2143			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCCTTCCGCCCTCGTGACTCTCCGGTCCACATGCTCGGGTGCTTTTCTTGCCGCTACTAA	PPFRPRDSPVHMLGCFSCRY*	-1.752	0.004147	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23794	CRLGRPYYFRFLIAFIGEPV			20	SLAY-screened peptide P2144			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAGGTTGGGCAGGCCGTATTATTTCCGTTTTCTTATCGCCTTCATTGGCGAGCCTGTCTAA	CRLGRPYYFRFLIAFIGEPV*	-1.751	0.000539	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23795	HAVTVFPGYSHNRNYSKSHY			20	SLAY-screened peptide P2145			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGCCGTCACCGTTTTTCCCGGCTATAGCCATAACCGTAACTATAGCAAGAGCCATTACTAA	HAVTVFPGYSHNRNYSKSHY*	-1.75	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23796	KLSG			4	SLAY-screened peptide P2146			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGTTGTCCGGTTAGCCGCCGCTTACGAAGCATACGGAGTATAAGATTACGAAGATCAAGTAA	KLSG*PPLTKHTEYKITKIK*	-1.75	0.003504	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23797	FPRASPIHQLKNRTLCTIFY			20	SLAY-screened peptide P2147			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCCCCGGGCGTCTCCCATCCATCAGCTTAAGAATCGTACGCTTTGCACTATTTTCTATTAA	FPRASPIHQLKNRTLCTIFY*	-1.749	0.007814	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23798	RILNSLSSRIYVLVALVKFR			20	SLAY-screened peptide P2148			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTATTTTGAACAGCCTTTCTTCCCGGATTTATGTGTTGGTTGCCCTTGTCAAGTTCCGTTAA	RILNSLSSRIYVLVALVKFR*	-1.749	0.000981	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23799	KVYNSTLNYTSPSLTPHPYC			20	SLAY-screened peptide P2149			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGGTGTATAACAGCACGCTGAATTACACGTCCCCCTCCTTGACTCCTCATCCTTATTGTTAA	KVYNSTLNYTSPSLTPHPYC*	-1.748	0.000425	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23800	YHYRCHRNSPPMPYSFTPRT			20	SLAY-screened peptide P2150			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCATTACCGCTGCCACCGGAATAGTCCCCCTATGCCTTATTCCTTTACTCCTCGCACTTAA	YHYRCHRNSPPMPYSFTPRT*	-1.748	4.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23801	SAGLYVARIHYQGFSMSHCS			20	SLAY-screened peptide P2151			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGCCGGCCTCTACGTCGCCCGGATCCATTACCAGGGCTTTAGTATGTCGCACTGCTCTTAA	SAGLYVARIHYQGFSMSHCS*	-1.748	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23802	LEKTHINSDSYFGPTDNICK			20	SLAY-screened peptide P2152			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGAGAAGACCCATATCAATAGCGACTCCTACTTCGGTCCCACGGACAATATTTGCAAGTAA	LEKTHINSDSYFGPTDNICK*	-1.748	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23803	LPYVATKGAFSDT			13	SLAY-screened peptide P2153			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCCTATGTGGCTACCAAGGGGGCTTTTTCCGATACTTAGGCTACGAGGAGTCACAGCTAA	LPYVATKGAFSDT*ATRSHS*	-1.748	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23804	MHPYISHFVIKLEASYVCSS			20	SLAY-screened peptide P2154			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCACCCGTACATCTCGCACTTCGTCATTAAGCTGGAGGCTTCCTATGTCTGTTCGTCGTAA	MHPYISHFVIKLEASYVCSS*	-1.747	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23805	TALSFVLQCLPCL			13	SLAY-screened peptide P2155			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGCTCTCTCCTTCGTCCTCCAGTGCCTGCCCTGCCTTTAGGTGCCGTTCTGCTTCTTGTAA	TALSFVLQCLPCL*VPFCFL*	-1.747	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23806	HRVNGHAHVLNPLVCGSSRS			20	SLAY-screened peptide P2156			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAGGGTTAACGGTCACGCCCACGTGCTTAATCCGCTTGTCTGCGGGTCGAGCAGGAGCTAA	HRVNGHAHVLNPLVCGSSRS*	-1.746	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23807	RGSQDLLILFHFSSPTSTDT			20	SLAY-screened peptide P2157			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGGGTTCCCAGGACCTTCTCATCCTGTTTCATTTCTCTAGTCCCACGTCGACTGACACCTAA	RGSQDLLILFHFSSPTSTDT*	-1.746	0.022835	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23808	PQRSRITVSSSRVYGALNVI			20	SLAY-screened peptide P2158			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCAGCGCTCTAGGATTACTGTTAGCTCTTCGCGTGTCTATGGGGCCCTTAATGTGATCTAA	PQRSRITVSSSRVYGALNVI*	-1.745	0.000694	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23809	ESRARSTHTVPR			12	SLAY-screened peptide P2159			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGTCGCGCGCTCGGAGTACCCATACGGTGCCCCGCTAGAGTTTGCTGCCTGATGTCGAGTAA	ESRARSTHTVPR*SLLPDVE*	-1.745	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23810	SDAHENTVVAVRFSWFNDPA			20	SLAY-screened peptide P2160			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGATGCCCACGAGAACACCGTCGTGGCCGTGCGTTTTTCGTGGTTTAATGACCCTGCGTAA	SDAHENTVVAVRFSWFNDPA*	-1.744	0.00553	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23811	GCPVYAPYPIYIGTSPMMRT			20	SLAY-screened peptide P2161			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTGCCCTGTTTACGCGCCGTACCCCATCTACATTGGTACTTCCCCCATGATGCGGACCTAA	GCPVYAPYPIYIGTSPMMRT*	-1.744	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23812	KLLFFLTHDGCNCVYTPLCVY			21	SLAY-screened peptide P2162			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCTTTTGTTTTTCCTGACGCATGATGGGTGTAATTGTGTTTATACTCCTTTGTGTGTGTAC	KLLFFLTHDGCNCVYTPLCVY	-1.744	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23813	LHAGPQFFNNLFDTACLPNN			20	SLAY-screened peptide P2163			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCACGCCGGCCCGCAGTTTTTCAATAATCTTTTTGACACCGCTTGTCTGCCTAATAATTAA	LHAGPQFFNNLFDTACLPNN*	-1.744	3.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23814	SLMDVCYYLL			10	SLAY-screened peptide P2164			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCTTATGGACGTGTGTTACTATCTGCTGTAGCCCCGCCCTTAGAGTGACCTCGTCGCCTAA	SLMDVCYYLL*PRP*SDLVA*	-1.743	4.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23815	ADLRGYLKEHKRYISIYYCP			20	SLAY-screened peptide P2165			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGACCTCCGCGGCTACCTCAAGGAGCACAAGCGCTATATTAGCATCTACTACTGTCCTTAA	ADLRGYLKEHKRYISIYYCP*	-1.743	8.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23816	WTYYVLALRPGSLTIGYTRT			20	SLAY-screened peptide P2166			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGACCTACTACGTCCTGGCCCTTCGGCCCGGTTCGCTTACTATTGGCTATACGCGGACGTAA	WTYYVLALRPGSLTIGYTRT*	-1.743	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23817	PIVTGSTHLTKKALSYGTSA			20	SLAY-screened peptide P2167			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATCGTCACGGGGTCCACGCATCTGACTAAGAAGGCCTTGAGTTACGGCACTTCTGCCTAA	PIVTGSTHLTKKALSYGTSA*	-1.742	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23818	TCTTDAQDNSSSVCATRQNA			20	SLAY-screened peptide P2168			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTGCACCACCGACGCTCAGGATAATTCTAGTAGTGTTTGCGCGACCCGCCAGAATGCGTAA	TCTTDAQDNSSSVCATRQNA*	-1.742	0.004084	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23819	QYEYLPNAHTTYTSISQSWI			20	SLAY-screened peptide P2169			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTATGAGTACTTGCCTAATGCCCATACCACTTATACTTCCATTAGTCAGTCCTGGATCTAA	QYEYLPNAHTTYTSISQSWI*	-1.742	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23820	VVRCFHSIWRPSSCGSYIIL			20	SLAY-screened peptide P2170			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGTTAGGTGTTTTCACAGCATCTGGCGGCCTAGTAGTTGCGGGTCGTATATCATCCTCTAA	VVRCFHSIWRPSSCGSYIIL*	-1.742	0.025366	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23821	CSTEVYNRSFEIHFLATLTP			20	SLAY-screened peptide P2171			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTCGACCGAGGTGTATAACCGTAGCTTCGAGATCCACTTTCTTGCTACTCTTACGCCTTAA	CSTEVYNRSFEIHFLATLTP*	-1.742	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23822	SPATLSHNFIRPLSACLTSY			20	SLAY-screened peptide P2172			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCCGGCCACGCTGAGTCACAATTTTATTCGTCCTCTCTCTGCCTGTCTGACCTCTTACTAG	SPATLSHNFIRPLSACLTSY*	-1.742	0.011457	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23823	NCSRPCCITVSMIGDARSTF			20	SLAY-screened peptide P2173			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTGTTCCCGCCCGTGCTGTATCACTGTTTCTATGATTGGCGACGCGCGGAGTACCTTCTAA	NCSRPCCITVSMIGDARSTF*	-1.742	3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23824	PSATRPPL			8	SLAY-screened peptide P2174			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCTGCTACTCGCCCTCCCCTTTAGCTCACTCCGCCTACGTGTACTAAGGCTTGCCGTTAA	PSATRPPL*LTPPTCTKACR*	-1.742	3.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23825	RAIPRHRSVTTPGKRPCNCN			20	SLAY-screened peptide P2175			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGCCATCCCTCGTCACCGCTCTGTCACCACCCCCGGCAAGCGTCCTTGTAATTGTAACTAA	RAIPRHRSVTTPGKRPCNCN*	-1.741	0.004644	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23826	PNIPKDT			7	SLAY-screened peptide P2176			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAACATTCCTAAGGACACTTAGTCTGTCACTCTTAAGATTGTCGATTACATGCCTGATTAA	PNIPKDT*SVTLKIVDYMPD*	-1.741	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23827	LHRNMLGTFAHPHKMQASID			20	SLAY-screened peptide P2177			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCACCGTAACATGCTCGGGACGTTTGCTCATCCCCACAAGATGCAGGCGAGCATTGACTAA	LHRNMLGTFAHPHKMQASID*	-1.741	0.000508	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23828	TAVCFRARFPQVSYALNTLG			20	SLAY-screened peptide P2178			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGCCGTCTGCTTCCGTGCCAGGTTTCCCCAGGTGAGTTACGCCCTGAATACTCTTGGCTAA	TAVCFRARFPQVSYALNTLG*	-1.741	0.009043	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23829	TELPQHNLTSTAFALRCFHT			20	SLAY-screened peptide P2179			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGAGCTGCCTCAGCACAACTTGACTTCCACGGCCTTTGCCCTGCGGTGTTTCCATACCTAA	TELPQHNLTSTAFALRCFHT*	-1.741	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23830	PGADLCNKLAYSTQYPYIPN			20	SLAY-screened peptide P2180			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGGTGCGGATCTCTGCAACAAGTTGGCCTATTCCACTCAGTACCCCTATATTCCCAACTAA	PGADLCNKLAYSTQYPYIPN*	-1.74	0.02154	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23831	HYNFR			5	SLAY-screened peptide P2181			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTATAATTTTCGTTAGTCGCGCATTACGTGTTCGACGGTGGTTCTGAGTACTCTCATTTAA	HYNFR*SRITCSTVVLSTLI*	-1.74	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23832	ICSPDMKARYNNPAPQTLTF			20	SLAY-screened peptide P2182			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTGTAGCCCGGATATGAAGGCCCGCTACAATAATCCCGCTCCTCAGACCTTGACCTTTTAA	ICSPDMKARYNNPAPQTLTF*	-1.74	2.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23833	HICYRETGTLPTITIPYSFI			20	SLAY-screened peptide P2183			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATATCTGTTACCGGGAGACGGGCACGTTGCCGACTATCACCATCCCTTATAGCTTTATTTAA	HICYRETGTLPTITIPYSFI*	-1.739	0.005552	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23834	VLPFRLDPRD			10	SLAY-screened peptide P2184			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCTGCCCTTTCGCCTGGACCCTAGGGACTAGATGGTCACGAGTGCTGTTAACGTTGCTTAA	VLPFRLDPRD*MVTSAVNVA*	-1.739	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23835	GRLQRVPIVCHAETFVDLAP			20	SLAY-screened peptide P2185			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCGTTTGCAGCGTGTGCCTATTGTCTGCCACGCGGAGACCTTTGTTGACCTGGCGCCCTAA	GRLQRVPIVCHAETFVDLAP*	-1.739	0.001977	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23836	PDRTPCTNPYNDFRTLHYIL			20	SLAY-screened peptide P2186			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGACCGTACCCCTTGTACCAACCCTTACAACGACTTCAGGACCCTGCACTACATCCTTTAA	PDRTPCTNPYNDFRTLHYIL*	-1.738	0.001513	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23837	VHNVNGQLYQAH			12	SLAY-screened peptide P2187			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCACAACGTCAATGGGCAGCTGTATCAGGCTCACTAGTTTACCGCCAGCGCCATCCCGTAA	VHNVNGQLYQAH*FTASAIP*	-1.738	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23838	EPTDDGHLQYFSSPSPLVVLT			21	SLAY-screened peptide P2188			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCCGACGGACGACGGGCACTTACAGTACTTTTCTTCTCCATCCCCCTTAGTTGTCCTAACT	EPTDDGHLQYFSSPSPLVVLT	-1.738	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23839	SFCNTSLLAPALFPP			15	SLAY-screened peptide P2189			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTTTTGTAATACGTCCTTGCTCGCCCCCGCGCTCTTCCCTCCTTAGGCCATCACGAGTTAA	SFCNTSLLAPALFPP*AITS*	-1.738	0.000257	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23840	RFVVATIKLTVESRILNVYWL			21	SLAY-screened peptide P2190			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTTTGTTGTGGCGACGATTAAGCTTACGGTGGAGTCCCGGATCCTTAATGTCTACTGGCTA	RFVVATIKLTVESRILNVYWL	-1.737	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23841	MFGQNILPSNLCTDPPDLDA			20	SLAY-screened peptide P2191			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTTTGGGCAGAACATCCTCCCTTCTAACTTGTGCACCGACCCTCCTGACTTGGATGCCTAA	MFGQNILPSNLCTDPPDLDA*	-1.737	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23842	SDRAATRVPLCHDHLLSYEE			20	SLAY-screened peptide P2192			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGACCGTGCGGCTACGAGGGTGCCTCTCTGCCATGATCATTTGCTTTCGTACGAGGAGTAA	SDRAATRVPLCHDHLLSYEE*	-1.737	0.003694	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23843	TRHYNPPTRTFMCHPAGAAP			20	SLAY-screened peptide P2193			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCGGCATTATAACCCGCCTACCCGTACCTTCATGTGTCATCCCGCCGGCGCCGCCCCTTAA	TRHYNPPTRTFMCHPAGAAP*	-1.737	0.010628	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23844	QSRFSGIHDVLHVALDWLFL			20	SLAY-screened peptide P2194			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTCGAGGTTTTCGGGTATTCATGATGTGCTTCATGTCGCTCTTGACTGGCTGTTCTTGTAA	QSRFSGIHDVLHVALDWLFL*	-1.736	0.000234	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23845	RMSPLYHALDLTGWYSI			17	SLAY-screened peptide P2195			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTATGTCTCCTCTTTATCACGCGCTTGACCTTACGGGCTGGTATTCTATTTAGTCTAACTAA	RMSPLYHALDLTGWYSI*SN*	-1.736	0.043012	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23846	QLLFSRLLLTRILLSLLRRN			20	SLAY-screened peptide P2196			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCTGCTGTTCTCTCGACTCTTACTTACCCGCATCCTACTAAGCCTTCTTCGCCGTAACTGA	QLLFSRLLLTRILLSLLRRN*	-1.734	0.003339	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23847	PRRRHIDAFIDST			13	SLAY-screened peptide P2197			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGTCGTCGGCATATTGACGCGTTCATCGACAGTACCTAGAATATGAACTACTGTTACTAA	PRRRHIDAFIDST*NMNYCY*	-1.734	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23848	TYYPATNIRANNPLQSQNMS			20	SLAY-screened peptide P2198			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTACTACCCGGCCACTAACATTCGGGCGAATAACCCGCTCCAGTCTCAGAACATGTCGTAA	TYYPATNIRANNPLQSQNMS*	-1.734	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23849	GYGQLFCRRDIMCNLKTASA			20	SLAY-screened peptide P2199			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTATGGGCAGCTCTTTTGTCGCCGCGATATTATGTGCAACTTGAAGACTGCTAGTGCGTAA	GYGQLFCRRDIMCNLKTASA*	-1.733	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23850	LQSNRHGHPTHANSFTHPPI			20	SLAY-screened peptide P2200			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCAGTCCAACAGGCACGGCCATCCCACCCATGCCAATTCTTTTACGCATCCGCCCATCTAA	LQSNRHGHPTHANSFTHPPI*	-1.733	5.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23851	SRHNVSNFVALNCAPHTDTF			20	SLAY-screened peptide P2201			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCGTCATAACGTCTCCAATTTCGTTGCCCTTAACTGTGCCCCGCACACCGACACGTTTTAA	SRHNVSNFVALNCAPHTDTF*	-1.733	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23852	NHKTTAGEPCKRCSKFSNCP			20	SLAY-screened peptide P2202			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCACAAGACTACGGCGGGGGAGCCTTGTAAGAGGTGCAGCAAGTTCTCGAATTGTCCCTAA	NHKTTAGEPCKRCSKFSNCP*	-1.732	0.00191	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23853	FITMPHPDNPGSKEDTHNNA			20	SLAY-screened peptide P2203			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTATTACTATGCCGCATCCTGACAACCCGGGTTCTAAGGAGGATACGCATAACAATGCCTAA	FITMPHPDNPGSKEDTHNNA*	-1.732	0.005869	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23854	LYDAIRSEHISPLLFTILFFN			21	SLAY-screened peptide P2204			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTACGACGCCATCCGGTCCGAGCATATAAGCCCTCTGCTCTTCACTATCCTTTTCTTTAAC	LYDAIRSEHISPLLFTILFFN	-1.732	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23855	NV			2	SLAY-screened peptide P2205			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGTGTAGTACTATGCCGCTGTTCGGATGCCTCTTTCCAACTGCCTTAGCGGTGAGCGCTAA	NV*YYAAVRMPLSNCLSGER*	-1.731	0.020523	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23856	RSEDCDRSTSCNFTCTSYDL			20	SLAY-screened peptide P2206			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTCCGAGGACTGCGACCGTAGTACTTCCTGTAATTTTACCTGCACCTCTTACGACCTTTAA	RSEDCDRSTSCNFTCTSYDL*	-1.731	0.00015	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23857	CPVRAHYAGFNLDNNMPYDR			20	SLAY-screened peptide P2207			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCCGTGCGCGCCCACTATGCTGGGTTTAATCTCGATAACAACATGCCCTATGATCGCTAA	CPVRAHYAGFNLDNNMPYDR*	-1.73	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23858	PRHRNLAI			8	SLAY-screened peptide P2208			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGCCACCGTAATCTGGCGATTTAGGATACTCCCTCTAGTAACCAGCAGGGCCATGGGTAA	PRHRNLAI*DTPSSNQQGHG*	-1.73	0.000166	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23859	VQSRVLRRIITCLFSRFVPV			20	SLAY-screened peptide P2209			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCAGAGCCGCGTGCTCAGGAGGATCATTACGTGTTTGTTCTCGAGGTTTGTTCCTGTTTAA	VQSRVLRRIITCLFSRFVPV*	-1.73	0.000224	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23860	WDYNSLSKLTITPWNYMQST			20	SLAY-screened peptide P2210			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGGATTACAATTCCCTGTCCAAGCTCACTATCACCCCCTGGAACTACATGCAGTCGACGTAA	WDYNSLSKLTITPWNYMQST*	-1.73	0.019816	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23861	DSRYSQSIQDFRPTRNSHNW			20	SLAY-screened peptide P2211			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTCGCGGTATTCCCAGTCTATTCAGGATTTTCGGCCGACGAGGAATTCCCACAATTGGTAA	DSRYSQSIQDFRPTRNSHNW*	-1.73	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23862	LSSLTLECLPLMTQFDHALSN			21	SLAY-screened peptide P2212			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGAGTTCGCTGACTCTTGAGTGTCTTCCCTTGATGACCCAGTTCGACCACGCACTAAGTAAC	LSSLTLECLPLMTQFDHALSN	-1.73	0.009395	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23863	RRLSHHLAFPNMRRITLAILN			21	SLAY-screened peptide P2213			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCGCCTCTCGCATCATCTTGCTTTTCCGAACATGAGGAGAATAACGCTAGCCATACTTAAC	RRLSHHLAFPNMRRITLAILN	-1.729	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23864	HPSESSNCHNLPFWPGSFIE			20	SLAY-screened peptide P2214			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCCTCTGAGAGCTCTAACTGTCATAACCTTCCTTTCTGGCCTGGCTCCTTCATTGAGTAA	HPSESSNCHNLPFWPGSFIE*	-1.729	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23865	HLTTALNGPYRVTMALDNIP			20	SLAY-screened peptide P2215			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCTTACCACGGCTCTTAATGGTCCGTACCGGGTTACGATGGCTCTTGACAACATCCCCTAA	HLTTALNGPYRVTMALDNIP*	-1.729	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23866	LHRNRSSRTPRTTYAGDHPP			20	SLAY-screened peptide P2216			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCACCGGAATAGGTCCTCCCGTACGCCGAGGACCACCTATGCCGGCGACCACCCTCCTTAA	LHRNRSSRTPRTTYAGDHPP*	-1.729	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23867	GYRIRIPGTVNNHDECYLLA			20	SLAY-screened peptide P2217			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTATCGCATTCGGATTCCCGGTACTGTCAATAATCACGATGAGTGCTATCTTTTGGCGTAA	GYRIRIPGTVNNHDECYLLA*	-1.729	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23868	IDVGQYQSIPKCVARMLI			18	SLAY-screened peptide P2218			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGACGTTGGCCAGTATCAGAGCATCCCTAAGTGTGTTGCCCGTATGCTCATTTAGTATTAA	IDVGQYQSIPKCVARMLI*Y*	-1.728	5.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23869	RPAYILEQPRGWVPTEQYLL			20	SLAY-screened peptide P2219			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCGGCTTACATCCTCGAGCAGCCTCGGGGGTGGGTTCCTACCGAGCAGTACCTTCTCTAA	RPAYILEQPRGWVPTEQYLL*	-1.728	0.039109	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23870	GLSVNHILALRLHRAFYLDP			20	SLAY-screened peptide P2220			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCTTTCCGTGAATCATATCCTTGCCCTTCGCCTTCACCGGGCCTTTTACCTTGACCCTTAA	GLSVNHILALRLHRAFYLDP*	-1.728	0.004898	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23871	TWHYHCQAPSIWSLTPSYSN			20	SLAY-screened peptide P2221			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTGGCATTACCACTGTCAGGCGCCCTCTATCTGGTCCCTCACTCCGAGTTACAGCAATTAA	TWHYHCQAPSIWSLTPSYSN*	-1.728	6.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23872	PDLTHSPWLNIPWSLVDACA			20	SLAY-screened peptide P2222			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGACCTTACTCACTCCCCGTGGCTCAACATCCCGTGGTCTCTTGTGGATGCCTGTGCCTAA	PDLTHSPWLNIPWSLVDACA*	-1.728	0.007056	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23873	LCSTYGPTRHGSPSQIPFHT			20	SLAY-screened peptide P2223			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTGCTCTACGTACGGCCCCACGCGCCACGGCAGCCCCTCCCAGATCCCTTTTCATACGTAA	LCSTYGPTRHGSPSQIPFHT*	-1.727	0.020993	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23874	RIPAKDHKQYRSTLCTVRRSN			21	SLAY-screened peptide P2224			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATCCCTGCTAAGGACCACAAGCAGTACCGAAGCACGTTATGTACTGTTAGGCGTAGTAAC	RIPAKDHKQYRSTLCTVRRSN	-1.726	0.000249	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23875	DVRSLVLLN			9	SLAY-screened peptide P2225			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGTCCGCAGTTTGGTGCTTCTCAATTAGAACCCCTCGCGCGCGCGTGCTTTTGTGAAGTAA	DVRSLVLLN*NPSRARAFVK*	-1.726	0.006629	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23876	PNPPNVWRLAHLDDPSTLTL			20	SLAY-screened peptide P2226			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAATCCGCCGAACGTTTGGCGCCTTGCCCATCTGGATGACCCTAGTACTCTTACGTTGTAA	PNPPNVWRLAHLDDPSTLTL*	-1.726	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23877	CLPNGVPTPNCYIVHFTALS			20	SLAY-screened peptide P2227			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTCCCCAATGGTGTTCCTACGCCTAACTGCTATATTGTTCATTTCACCGCCCTTTCCTAA	CLPNGVPTPNCYIVHFTALS*	-1.725	0.000446	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23878	SSPCALYSQISITFLNWVRT			20	SLAY-screened peptide P2228			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTAGCCCCTGTGCGCTCTATTCGCAGATTTCTATCACCTTCCTTAATTGGGTTCGTACTTAA	SSPCALYSQISITFLNWVRT*	-1.725	0.000119	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23879	TCHAHCLPRNLPTIPICLYR			20	SLAY-screened peptide P2229			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTGTCATGCCCATTGCTTGCCTCGTAATTTGCCGACGATTCCGATTTGCCTCTACCGTTAA	TCHAHCLPRNLPTIPICLYR*	-1.725	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23880	IPPTRRSNTSHDS			13	SLAY-screened peptide P2230			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCCCCCTACCAGGCGGAGTAATACCTCCCATGACTCTTAGGATATCACTGACTAGCGGTAA	IPPTRRSNTSHDS*DITD*R*	-1.724	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23881	QNNDV			5	SLAY-screened peptide P2231			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGAACAATGATGTTTAGAACTGGCGTGTCTACCGTTCCTGTCGCTACACTTATACGGATTAA	QNNDV*NWRVYRSCRYTYTD*	-1.724	0.014278	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23882	PTAVVTSPVPNPFNVYSYTI			20	SLAY-screened peptide P2232			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACCGCCGTGGTCACCAGCCCCGTTCCTAACCCCTTTAACGTTTATTCTTATACTATTTAA	PTAVVTSPVPNPFNVYSYTI*	-1.724	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23883	PPQPQFGIVRSGVGVHQIGI			20	SLAY-screened peptide P2233			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCTCAGCCTCAGTTTGGCATTGTGCGCTCGGGGGTGGGCGTGCACCAGATCGGTATTTAA	PPQPQFGIVRSGVGVHQIGI*	-1.724	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23884	PRFSKCIRWNIQHLPIT			17	SLAY-screened peptide P2234			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGCTTCTCCAAGTGTATCCGTTGGAATATCCAGCATCTCCCCATTACCTAGATCCGCTAA	PRFSKCIRWNIQHLPIT*IR*	-1.724	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23885	LNSITVLLRLILNVVLLCFS			20	SLAY-screened peptide P2235			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGAATAGCATCACTGTGCTGCTCCGCTTGATCCTTAACGTCGTTTTGCTTTGCTTTTCCTAA	LNSITVLLRLILNVVLLCFS*	-1.723	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23886	YRPTNANVTTHATLALAVPC			20	SLAY-screened peptide P2236			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCGCCCTACGAATGCGAACGTTACCACCCACGCGACTCTGGCTCTCGCGGTCCCGTGCTAA	YRPTNANVTTHATLALAVPC*	-1.723	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23887	TLHLCALHF			9	SLAY-screened peptide P2237			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTCCACCTGTGCGCTCTGCATTTTTAGCTGAACCCTCGTCATTCCTTTTAGGTTCCTTAA	TLHLCALHF*LNPRHSF*VP*	-1.723	0.001475	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23888	HPGAFPPR			8	SLAY-screened peptide P2238			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCCGGCGCTTTTCCCCCGCGCTAGCACCACATCGTTATTAGTCACGGGCGTAAGTCGTAC	HPGAFPPR*HHIVISHGRKSY	-1.723	0.001259	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23889	PQRPTYNNTCTTRSHYLPGY			20	SLAY-screened peptide P2239			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCAGCGCCCTACCTACAATAATACTTGTACTACGCGCTCGCATTATCTCCCCGGTTACTAA	PQRPTYNNTCTTRSHYLPGY*	-1.723	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23890	RPRRQSVHEQCHATPIATSCN			21	SLAY-screened peptide P2240			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCTCGCCGTCAGAGCGTCCACGAGCAGTGCCATGCTACTCCTATCGCCACGAGCTGTAAC	RPRRQSVHEQCHATPIATSCN	-1.722	0.033116	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23891	KPVYIRSSPYCHMGDRRATA			20	SLAY-screened peptide P2241			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCCTGTCTATATTCGGAGTAGCCCCTACTGTCATATGGGTGATCGGCGTGCGACGGCCTAA	KPVYIRSSPYCHMGDRRATA*	-1.721	0.001061	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23892	MSGTHGWLMEPLFHVLLACA			20	SLAY-screened peptide P2242			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTCTGGTACTCACGGTTGGCTCATGGAGCCGTTGTTTCACGTCCTGCTTGCTTGTGCTTAA	MSGTHGWLMEPLFHVLLACA*	-1.721	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23893	KSYSE			5	SLAY-screened peptide P2243			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGAGCTATTCGGAGTAGAGCATCGCCAGGCACCTGAGTGGCACCTATCACATTGACTTGTAA	KSYSE*SIARHLSGTYHIDL*	-1.72	0.000502	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23894	LVKMNRSLILTVINRTNRRW			20	SLAY-screened peptide P2244			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGTCAAGATGAACCGCAGCCTGATCTTGACTGTCATCAACCGGACCAACAGGCGCTGGTAA	LVKMNRSLILTVINRTNRRW*	-1.72	0.015724	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23895	SARNHQPYTPICLGAASLSI			20	SLAY-screened peptide P2245			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCGCGTAATCACCAGCCTTATACTCCTATCTGCTTGGGTGCTGCTTCTCTTTCTATTTAA	SARNHQPYTPICLGAASLSI*	-1.719	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23896	PCSMPPYWWGIAVVPTIEIV			20	SLAY-screened peptide P2246			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGCTCGATGCCCCCTTACTGGTGGGGTATCGCTGTTGTCCCCACTATCGAGATTGTGTAA	PCSMPPYWWGIAVVPTIEIV*	-1.719	4.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23897	PLYEAGTIHFPLQP			14	SLAY-screened peptide P2247			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTGTATGAGGCCGGCACCATCCATTTCCCTTTGCAGCCTTAGCATACGAATAACGTGTAA	PLYEAGTIHFPLQP*HTNNV*	-1.719	0.002428	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23898	RYVPTMHITSCTDWAGCHYL			20	SLAY-screened peptide P2248			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTATGTCCCTACCATGCACATTACGAGTTGTACTGACTGGGCGGGTTGCCACTATCTCTAA	RYVPTMHITSCTDWAGCHYL*	-1.719	0.001062	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23899	KPH			3	SLAY-screened peptide P2249			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCCTCATTAGCATCCTATCAACTTCTACCTGTAGCATTACGATTAGATGGATGTTTTGTAA	KPH*HPINFYL*HYD*MDVL*	-1.718	0.004391	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23900	RSGRTQCYNRNYHPFIIEVL			20	SLAY-screened peptide P2250			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTCTGGTCGGACCCAGTGCTACAACCGGAATTATCACCCTTTTATTATTGAGGTCTTGTAA	RSGRTQCYNRNYHPFIIEVL*	-1.718	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23901	SYHISLASISTSSYGQYPHVT			21	SLAY-screened peptide P2251			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTACCATATATCCCTTGCTTCTATTAGTACATCGTCATACGGGCAGTACCCCCATGTAACT	SYHISLASISTSSYGQYPHVT	-1.718	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23902	PLRMLTDKKPSFVIMEKFHT			20	SLAY-screened peptide P2252			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCCGGATGTTGACTGACAAGAAGCCTAGTTTTGTCATCATGGAGAAGTTCCATACTTAA	PLRMLTDKKPSFVIMEKFHT*	-1.718	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23903	RLSRPENPPVRDYILLSWGC			20	SLAY-screened peptide P2253			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTTAGTCGTCCGGAGAATCCTCCCGTCCGTGATTATATCCTTTTGTCTTGGGGCTGCTAA	RLSRPENPPVRDYILLSWGC*	-1.717	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23904	TCSSFHLRWRALDTTSRCRL			20	SLAY-screened peptide P2254			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTGTTCGTCTTTTCACCTCCGTTGGAGGGCGTTGGATACGACCTCCCGCTGCCGTCTTTAA	TCSSFHLRWRALDTTSRCRL*	-1.717	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23905	CRWNYRYPYFYRI			13	SLAY-screened peptide P2255			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGTTGGAACTACAGGTACCCGTACTTCTACCGTATCTAGCACCGCAGGAACACCTTCTAA	CRWNYRYPYFYRI*HRRNTF*	-1.717	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23906	GSQYACFFYQSGKLLVRTI			19	SLAY-screened peptide P2256			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGAGTCAGTACGCGTGTTTTTTTTATCAGAGTGGCAAGTTGCTTGTTAGGACTATTTAGTAA	GSQYACFFYQSGKLLVRTI**	-1.717	0.000934	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23907	QVPSWFNPGVYRQILL			16	SLAY-screened peptide P2257			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGTCCCCTCTTGGTTCAACCCTGGCGTCTATCGTCAGATTCTGCTTTAGTGCCTGGCTTAA	QVPSWFNPGVYRQILL*CLA*	-1.717	0.005903	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23908	TTASPVCHLVPRYTPNTTIS			20	SLAY-screened peptide P2258			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACGGCTTCGCCCGTTTGCCACCTTGTTCCTAGGTACACGCCGAATACTACTATTTCCTAA	TTASPVCHLVPRYTPNTTIS*	-1.716	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23909	ITNHSYFPRLGTHSTQTSSC			20	SLAY-screened peptide P2259			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTACCAATCACAGTTATTTCCCGCGCTTGGGCACTCACTCGACCCAGACGTCCTCCTGCTAA	ITNHSYFPRLGTHSTQTSSC*	-1.716	0.00655	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23910	TLARLTVDVSNLKKNHDGLS			20	SLAY-screened peptide P2260			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTTGCCAGGCTGACTGTGGATGTCAGCAACTTGAAGAAGAATCATGACGGTTTGTCGTAA	TLARLTVDVSNLKKNHDGLS*	-1.716	0.012844	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23911	CNSPGTDPEPQYCVPYHKDP			20	SLAY-screened peptide P2261			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAACTCGCCGGGCACCGACCCTGAGCCCCAGTATTGCGTCCCGTATCATAAGGATCCTTAA	CNSPGTDPEPQYCVPYHKDP*	-1.716	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23912	ALLTASLVLRARIRLSMRNN			20	SLAY-screened peptide P2262			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCTCCTCACTGCCAGTCTTGTCCTGCGTGCCCGCATTCGTCTGTCCATGAGGAATAACTAA	ALLTASLVLRARIRLSMRNN*	-1.716	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23913	GRLPNHNGVPPSLATTGQCS			20	SLAY-screened peptide P2263			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCGCCTTCCGAACCACAATGGGGTTCCCCCTAGTTTGGCTACTACTGGCCAGTGCTCCTAA	GRLPNHNGVPPSLATTGQCS*	-1.716	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23914	LALAEHSTSMYPLRNRSSPW			20	SLAY-screened peptide P2264			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGCTCTCGCGGAGCACTCGACCAGTATGTATCCTTTGCGTAATCGCTCCTCGCCGTGGTAA	LALAEHSTSMYPLRNRSSPW*	-1.715	1.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23915	AIQRELNSTRYLSIARLSSLN			21	SLAY-screened peptide P2265			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCATTCAGCGTGAGCTTAACTCTACGCGGTACCTCTCAATAGCCAGACTAAGCAGCCTTAAC	AIQRELNSTRYLSIARLSSLN	-1.715	0.000313	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23916	GYTPESDLPKQYLMQFLFAY			20	SLAY-screened peptide P2266			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTATACGCCCGAGAGTGACCTCCCGAAGCAGTATCTTATGCAGTTCCTTTTTGCCTATTAA	GYTPESDLPKQYLMQFLFAY*	-1.715	0.030238	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23917	YTPPYSYLLMKTHRCIPSLI			20	SLAY-screened peptide P2267			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACGCCCCCCTACAGTTATCTTCTTATGAAGACTCACCGTTGTATTCCCTCTCTCATCTAA	YTPPYSYLLMKTHRCIPSLI*	-1.715	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23918	RSRSRGCFLGCTLDASPNAS			20	SLAY-screened peptide P2268			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAGCAGGAGCCGCGGCTGCTTCCTCGGTTGTACTCTTGATGCGAGCCCGAATGCCTCGTAA	RSRSRGCFLGCTLDASPNAS*	-1.714	0.00569	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23919	LPTPSDYILPRGPPCRIKPS			20	SLAY-screened peptide P2269			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCCACTCCCAGTGATTATATTCTTCCCCGCGGCCCTCCCTGCCGTATTAAGCCGTCGTAA	LPTPSDYILPRGPPCRIKPS*	-1.714	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23920	STHRMCPFRNPYLQLTAYFK			20	SLAY-screened peptide P2270			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCACGCACCGCATGTGTCCGTTCAGGAACCCTTACTTGCAGCTGACGGCGTATTTCAAGTAA	STHRMCPFRNPYLQLTAYFK*	-1.714	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23921	ITFHYHWYF			9	SLAY-screened peptide P2271			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTACTTTCCATTACCACTGGTACTTCTAGAGGTCGCACCATACCTTTAAGCACGGTTTTTAA	ITFHYHWYF*RSHHTFKHGF*	-1.714	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23922	SPCRRLRLRRSPIAIDFYSV			20	SLAY-screened peptide P2272			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCCTTGCCGGCGTCTGCGCCTGCGTCGTAGTCCCATCGCTATCGATTTCTATAGCGTGTAA	SPCRRLRLRRSPIAIDFYSV*	-1.713	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23923	THVPIDISSPTFTVVLLLLM			20	SLAY-screened peptide P2273			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCATGTTCCCATCGACATTTCGTCGCCGACTTTTACTGTGGTTCTCTTGCTGCTTATGTAA	THVPIDISSPTFTVVLLLLM*	-1.713	4.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23924	SQPFVDAPGSKFQTHDIMRN			20	SLAY-screened peptide P2274			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCAGCCGTTTGTGGACGCGCCCGGTTCGAAGTTTCAGACGCATGATATCATGAGGAACTAA	SQPFVDAPGSKFQTHDIMRN*	-1.713	0.000605	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23925	LCRATPPQSATDAAVYFNHR			20	SLAY-screened peptide P2275			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTGCCGTGCGACCCCCCCCCAGTCGGCTACTGACGCTGCCGTGTATTTTAACCATCGGTAA	LCRATPPQSATDAAVYFNHR*	-1.713	0.001947	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23926	QGYS			4	SLAY-screened peptide P2276			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGGCTACAGTTAGGCTCCGCCGGTCCATGATACTACCAATGCGGCTCCTAACCATGTCTAA	QGYS*APPVHDTTNAAPNHV*	-1.713	6.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23927	SRRIDYESNSEEAQVITT			18	SLAY-screened peptide P2277			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCGCCGTATCGATTATGAGAGCAACTCTGAGGAGGCCCAGGTGATTACTACTTAGTCCTAA	SRRIDYESNSEEAQVITT*S*	-1.713	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23928	LLEYGYDKLRLGAGLYSPIH			20	SLAY-screened peptide P2278			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTCGAGTATGGTTATGATAAGCTGCGCTTGGGTGCCGGGCTCTACAGCCCTATCCATTAA	LLEYGYDKLRLGAGLYSPIH*	-1.713	4.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23929	RYNCNSDCYTTVYHDSFTDS			20	SLAY-screened peptide P2279			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTATAATTGCAACTCGGACTGCTATACTACTGTCTATCATGATAGTTTCACCGACTCCTAA	RYNCNSDCYTTVYHDSFTDS*	-1.712	5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23930	SVADNPPWIAPICAPFAFSIN			21	SLAY-screened peptide P2280			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGTCGCTGACAACCCTCCTTGGATCGCGCCCATCTGCGCACCCTTTGCGTTTTCGATTAAC	SVADNPPWIAPICAPFAFSIN	-1.712	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23931	LRKLILWIVATRWPFVRRSFN			21	SLAY-screened peptide P2281			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCGTAAGCTTATCCTGTGGATAGTTGCCACGCGCTGGCCATTTGTTCGCAGAAGCTTTAAC	LRKLILWIVATRWPFVRRSFN	-1.712	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23932	LPPALRMQVFIPITSVITNPN			21	SLAY-screened peptide P2282			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCCCCGGCGCTCCGCATGCAGGTTTTTATTCCCATCACCAGTGTTATCACTAATCCTAAC	LPPALRMQVFIPITSVITNPN	-1.712	0.000602	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23933	PSQAPNTRVDSASCPGRAKP			20	SLAY-screened peptide P2283			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGTCAGGCCCCTAATACTCGTGTTGACTCTGCGAGTTGTCCGGGGCGTGCTAAGCCTTAA	PSQAPNTRVDSASCPGRAKP*	-1.712	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23934	LTSTSINRVRLSRASMTFLM			20	SLAY-screened peptide P2284			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACTAGTACCAGTATCAATCGTGTGCGTCTTTCCCGTGCTTCTATGACTTTTTTGATGTAA	LTSTSINRVRLSRASMTFLM*	-1.711	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23935	SSRPAAIFQPRTYFNSLALS			20	SLAY-screened peptide P2285			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTCTCGCCCCGCCGCTATTTTTCAGCCGCGTACCTACTTCAACAGCCTCGCGTTGTCCTAA	SSRPAAIFQPRTYFNSLALS*	-1.711	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23936	DAHQNGVTLILCVPQHNSIA			20	SLAY-screened peptide P2286			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGCTCATCAGAACGGCGTCACGCTCATTCTGTGCGTGCCGCAGCATAATTCGATCGCTTAA	DAHQNGVTLILCVPQHNSIA*	-1.711	0.021184	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23937	PTSSYWPVRT			10	SLAY-screened peptide P2287			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACCTCTAGTTATTGGCCTGTTAGGACGTAGAGCCTGCTTTGCTAGCTCCTCTGCCCCTAA	PTSSYWPVRT*SLLC*LLCP*	-1.711	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23938	MSSVDCHHNKYPPVIACIPS			20	SLAY-screened peptide P2288			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTCTAGTGTTGATTGCCACCACAACAAGTACCCTCCGGTCATTGCTTGCATTCCCAGCTAA	MSSVDCHHNKYPPVIACIPS*	-1.711	0.014549	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23939	LLGYILSILYDLIFLWSTCL			20	SLAY-screened peptide P2289			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCTCGGGTACATTCTTTCGATCTTGTACGATCTTATTTTCCTCTGGAGCACCTGTCTCTAA	LLGYILSILYDLIFLWSTCL*	-1.711	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23940	HIKETPPIPSVFLLGDHALI			20	SLAY-screened peptide P2290			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATATTAAGGAGACGCCGCCCATTCCGTCGGTTTTCCTTCTCGGCGACCATGCTCTTATCTAA	HIKETPPIPSVFLLGDHALI*	-1.711	0.013553	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23941	TIVAPSVPPRRLMILPPHVA			20	SLAY-screened peptide P2291			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCATCGTCGCCCCCTCGGTGCCGCCTAGGCGCTTGATGATCTTGCCCCCTCACGTCGCGTAA	TIVAPSVPPRRLMILPPHVA*	-1.71	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23942	NNSTWDHPSHNIVGVLIFMFT			21	SLAY-screened peptide P2292			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATAATTCTACTTGGGATCACCCCTCTCATAATATTGTCGGTGTTTTGATTTTTATGTTTACT	NNSTWDHPSHNIVGVLIFMFT	-1.71	0.00543	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23943	GYYCYRAALRVPASSAYPFH			20	SLAY-screened peptide P2293			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGTACTATTGCTATCGGGCCGCCCTTCGGGTGCCGGCGTCTAGTGCCTACCCTTTCCATTAA	GYYCYRAALRVPASSAYPFH*	-1.71	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23944	NSIALSGHEWTQAGRTGNLA			20	SLAY-screened peptide P2294			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTCTATCGCTCTCTCTGGTCACGAGTGGACGCAGGCGGGCCGCACTGGGAATCTCGCGTAA	NSIALSGHEWTQAGRTGNLA*	-1.709	4.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23945	IHDSGRKSNSESLAPCYYNV			20	SLAY-screened peptide P2295			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCACGACAGTGGCCGGAAGAGTAACTCTGAGAGTCTGGCTCCTTGTTACTATAACGTCTAA	IHDSGRKSNSESLAPCYYNV*	-1.709	0.005877	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23946	SHSYRSLPCFTT			12	SLAY-screened peptide P2296			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCACTCGTACCGGAGTTTGCCGTGTTTCACCACCTAGCCTATTCGCGCTCTCACGGTTTAA	SHSYRSLPCFTT*PIRALTV*	-1.709	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23947	CRISCVFLPSRPTYLRMPLL			20	SLAY-screened peptide P2297			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAGGATCTCTTGTGTTTTCCTCCCCAGCCGCCCCACGTATTTGCGTATGCCCTTGCTTTAA	CRISCVFLPSRPTYLRMPLL*	-1.709	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23948	PVCATAARTPNLYHLADPST			20	SLAY-screened peptide P2298			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGTTTGCGCCACTGCCGCTCGTACGCCTAATTTGTATCACCTCGCGGACCCTAGCACCTAA	PVCATAARTPNLYHLADPST*	-1.708	0.000749	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23949	AQPYT			5	SLAY-screened peptide P2299			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCAGCCTTACACCTAGGATACTTCGGATAAGCAGAACTCTAAGAATTACCGCCTGCGCTAA	AQPYT*DTSDKQNSKNYRLR*	-1.708	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23950	LIYGYFLRLYCSPMSSMI			18	SLAY-screened peptide P2300			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCATTTATGGGTATTTCCTTCGGCTGTATTGCTCCCCGATGTCCTCCATGATTTAGGTGTAA	LIYGYFLRLYCSPMSSMI*V*	-1.708	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23951	AQVRLARISYTSPLPSLPRGN			21	SLAY-screened peptide P2301			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCAGGTTAGGCTTGCTAGGATCTCTTACACCAGCCCGCTACCTTCATTACCACGCGGTAAC	AQVRLARISYTSPLPSLPRGN	-1.707	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23952	PDNEQLTNCTNHSLFAKFTA			20	SLAY-screened peptide P2302			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGATAACGAGCAGCTCACTAACTGCACTAATCACAGCCTTTTTGCCAAGTTCACTGCCTAA	PDNEQLTNCTNHSLFAKFTA*	-1.707	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23953	LHPPNITPSVDSNY			14	SLAY-screened peptide P2303			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCACCCCCCCAACATCACGCCCTCCGTGGACTCTAATTATTAGAACTTGGATGTTTTTTAA	LHPPNITPSVDSNY*NLDVF*	-1.707	0.000899	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23954	VAPAGPLPL			9	SLAY-screened peptide P2304			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGCTCCTGCTGGTCCTCTGCCGTTGTAGTAGGGTTTTTTTCTTTATCCTCCTATCGGTTAA	VAPAGPLPL**GFFLYPPIG*	-1.707	0.000156	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23955	PIEYLLVSLNHHYLSHA			17	SLAY-screened peptide P2305			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATCGAGTATTTGCTGGTCAGCCTGAATCACCATTATTTGAGTCATGCTTAGGAGCTTTAA	PIEYLLVSLNHHYLSHA*EL*	-1.707	0.002435	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23956	SHNITSSVCYHLYWDFSSLR			20	SLAY-screened peptide P2306			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCATAATATCACTAGTAGCGTTTGTTACCATCTTTACTGGGACTTCTCTAGCCTCCGGTAA	SHNITSSVCYHLYWDFSSLR*	-1.706	0.007143	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23957	LRVGFFSIHSTRRKRHR			17	SLAY-screened peptide P2307			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCGCGTCGGGTTCTTTAGTATTCACTCCACGAGGCGCAAGCGGCATCGGTAGTATAGCTAA	LRVGFFSIHSTRRKRHR*YS*	-1.706	0.001085	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23958	PPYRSSVSRFTN			12	SLAY-screened peptide P2308			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCTTACCGTTCGAGCGTTTCTCGCTTCACCAATTAGGCGTCTTTTTTGGATGCTGACTAA	PPYRSSVSRFTN*ASFLDAD*	-1.706	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23959	GFSTMPVPLCIFPSPWWTSI			20	SLAY-screened peptide P2309			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTTTTCCACTATGCCCGTGCCTTTGTGTATCTTTCCTTCTCCTTGGTGGACCAGCATCTAA	GFSTMPVPLCIFPSPWWTSI*	-1.706	0.021577	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23960	HIYTDGPDPHNVYCNSICRH			20	SLAY-screened peptide P2310			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATATCTATACTGACGGCCCGGATCCTCACAACGTTTATTGTAACAGCATTTGTCGCCACTAA	HIYTDGPDPHNVYCNSICRH*	-1.705	0.000267	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23961	VGPALPRSLPIGIYVTPLSS			20	SLAY-screened peptide P2311			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGGCCCGGCCCTTCCCCGCTCGCTTCCGATCGGTATCTATGTCACGCCTTTGTCGTCCTAA	VGPALPRSLPIGIYVTPLSS*	-1.705	0.02888	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23962	LSCSSDSRNNGLSCTY			16	SLAY-screened peptide P2312			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTCCTGCTCTTCTGATAGCCGCAACAACGGCCTGAGCTGCACTTACTAGCTTCGCTGCTAA	LSCSSDSRNNGLSCTY*LRC*	-1.705	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23963	PCRKNFSLRCFVSNLGDNML			20	SLAY-screened peptide P2313			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGCCGTAAGAATTTTTCCTTGCGGTGTTTCGTCAGTAATCTGGGCGACAACATGCTCTAA	PCRKNFSLRCFVSNLGDNML*	-1.705	0.002989	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23964	PLPVRTMSFTVPRSCTSIPS			20	SLAY-screened peptide P2314			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTTCCCGTTAGGACCATGAGTTTCACGGTGCCTCGCTCTTGCACTTCCATCCCGTCCTAA	PLPVRTMSFTVPRSCTSIPS*	-1.705	0.005139	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23965	TSYLRCSYCAGGMYGLRRSD			20	SLAY-screened peptide P2315			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTCTTACCTCCGTTGTTCTTATTGCGCCGGTGGTATGTATGGCCTGCGCCGGTCCGACTAA	TSYLRCSYCAGGMYGLRRSD*	-1.704	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23966	SSTGSSNRATYAYTSATHKP			20	SLAY-screened peptide P2316			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTCTACTGGTAGCTCGAACAGGGCCACGTATGCTTACACGTCTGCTACTCATAAGCCCTAA	SSTGSSNRATYAYTSATHKP*	-1.704	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23967	RD			2	SLAY-screened peptide P2317			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGATTAGTGTTATCGTTTGGACCATGAGTCCCCTGGTCGCAACTACTTTTAGCGTCCGTAA	RD*CYRLDHESPGRNYF*RP*	-1.704	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23968	CMRVLVLFRWRRSLRDL			17	SLAY-screened peptide P2318			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTATGAGGGTCCTGGTTCTGTTCAGGTGGCGCCGGAGTCTCCGTGATCTGTAGACCATGTAA	CMRVLVLFRWRRSLRDL*TM*	-1.704	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23969	QTNLSMDSHYSIAQPLCHSL			20	SLAY-screened peptide P2319			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGACCAATCTGAGTATGGACTCCCATTATTCTATTGCTCAGCCCCTCTGCCATTCCCTTTAA	QTNLSMDSHYSIAQPLCHSL*	-1.704	0.005167	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23970	CNTIRDKWVQMLYAMPTDYL			20	SLAY-screened peptide P2320			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAACACTATTCGCGACAAGTGGGTCCAGATGCTCTATGCGATGCCGACTGACTATCTTTAA	CNTIRDKWVQMLYAMPTDYL*	-1.703	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23971	NDPRLPSYYRHFLSLGFTTD			20	SLAY-screened peptide P2321			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGATCCGCGCTTGCCGAGCTACTATCGGCATTTCTTGTCGCTGGGTTTCACCACTGATTAA	NDPRLPSYYRHFLSLGFTTD*	-1.703	0.023868	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23972	SGRNGRTHGHHGTNRLLVRD			20	SLAY-screened peptide P2322			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGGGCGCAACGGTCGGACCCATGGGCATCATGGCACCAACCGGCTCTTGGTCCGTGATTAA	SGRNGRTHGHHGTNRLLVRD*	-1.703	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23973	ATPRFCNGVGCCWSSWILL			19	SLAY-screened peptide P2323			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTACCCCTCGTTTTTGTAATGGCGTTGGTTGCTGTTGGTCGAGCTGGATCCTTCTGTAGTAA	ATPRFCNGVGCCWSSWILL**	-1.703	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23974	RNVVGPYRCRACNALRINNT			20	SLAY-screened peptide P2324			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAATGTCGTCGGCCCTTATCGCTGTAGGGCGTGCAACGCTCTTCGCATCAACAATACGTAA	RNVVGPYRCRACNALRINNT*	-1.703	9.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23975	LQLTAFLKLVYRLISTYASP			20	SLAY-screened peptide P2325			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCAGCTTACTGCCTTTTTGAAGCTTGTTTACCGTCTTATTTCGACCTACGCTTCCCCTTAA	LQLTAFLKLVYRLISTYASP*	-1.702	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23976	RPPLPSSVLSFQAPHIYTLS			20	SLAY-screened peptide P2326			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCGCCCCTTCCGTCCTCGGTTCTTAGCTTCCAGGCCCCTCATATCTACACCCTTTCCTAA	RPPLPSSVLSFQAPHIYTLS*	-1.702	4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23977	RVHDFPWGSAGLSYNVSPITN			21	SLAY-screened peptide P2327			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGTTCACGATTTTCCTTGGGGCTCGGCCGGTCTCAGTTATAACGTGTCGCCTATAACTAAC	RVHDFPWGSAGLSYNVSPITN	-1.701	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23978	CRGAATPMYLNRSRRHYAGL			20	SLAY-screened peptide P2328			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGGGGGGCTGCGACCCCCATGTACTTGAACCGCTCGCGCCGTCATTATGCGGGCTTGTAA	CRGAATPMYLNRSRRHYAGL*	-1.701	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23979	LDLHLSPIFAPLCYSFLGSL			20	SLAY-screened peptide P2329			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGACCTTCACCTTTCGCCGATTTTCGCTCCTCTTTGCTATTCTTTCCTGGGGAGTCTTTAA	LDLHLSPIFAPLCYSFLGSL*	-1.701	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23980	IPFIYF			6	SLAY-screened peptide P2330			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCCCTTCATCTACTTTTAGGATACGCACACGGGCGTGATCGACAAGGCTTAGTAGGCTTAA	IPFIYF*DTHTGVIDKA**A*	-1.701	0.010875	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23981	AQPPADSRPAPNLFL			15	SLAY-screened peptide P2331			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCAGCCGCCCGCTGATTCTCGTCCGGCTCCGAACCTTTTCCTGTAGAGTTCCCTCTTAGTA	AQPPADSRPAPNLFL*SSLLV	-1.701	0.016131	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23982	HGNNTTRTSFKLSRLTTFFV			20	SLAY-screened peptide P2332			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGGTAACAACACCACGCGCACCAGCTTCAAGTTGTCCCGTCTCACGACTTTCTTCGTCTAA	HGNNTTRTSFKLSRLTTFFV*	-1.701	0.020868	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23983	RILRGRARHFGNAKRPLTSR			20	SLAY-screened peptide P2333			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGATCCTGCGGGGCCGCGCCCGCCACTTTGGCAACGCTAAGCGGCCCCTGACCTCTCGTTAA	RILRGRARHFGNAKRPLTSR*	-1.701	7.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23984	IHPHLLHCKNLFTRYNKYTH			20	SLAY-screened peptide P2334			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCACCCCCATCTGCTCCATTGTAAGAACCTTTTTACGCGCTATAACAAGTATACCCATTAA	IHPHLLHCKNLFTRYNKYTH*	-1.701	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23985	YYHTNHAYCDSFKITYEWLP			20	SLAY-screened peptide P2335			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTACCATACCAACCACGCCTACTGTGACTCTTTTAAGATCACCTATGAGTGGCTCCCGTAA	YYHTNHAYCDSFKITYEWLP*	-1.701	0.029454	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23986	RSRRLVFRLINLFCPMCFSS			20	SLAY-screened peptide P2336			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCCCGCCGCCTGGTGTTCCGTCTGATTAATCTCTTTTGCCCTATGTGTTTTTCCTCCTAA	RSRRLVFRLINLFCPMCFSS*	-1.7	0.000189	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23987	PPNHQWRQMTTYSCRTSRAN			20	SLAY-screened peptide P2337			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCAACCACCAGTGGCGTCAGATGACTACTTACTCGTGTCGGACCTCTCGGGCTAATTAA	PPNHQWRQMTTYSCRTSRAN*	-1.7	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23988	GPAYTIHSVLSTSPTRLWSP			20	SLAY-screened peptide P2338			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCCCGCTTATACGATTCACTCGGTCCTTAGCACTTCTCCCACCAGGTTGTGGAGTCCGTAA	GPAYTIHSVLSTSPTRLWSP*	-1.7	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23989	SPKQPVASSCRPRRALPEELT			21	SLAY-screened peptide P2339			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCCCAAGCAGCCCGTTGCCAGTAGTTGCCGTCCGCGTCGCGCGCTCCCTGAAGAGCTAACT	SPKQPVASSCRPRRALPEELT	-1.7	7.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23990	PGWNNTKRYRKRLPLYRCMN			20	SLAY-screened peptide P2340			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGGTTGGAATAACACTAAGCGTTACCGCAAGCGTCTTCCCCTTTACCGCTGCATGAATTAA	PGWNNTKRYRKRLPLYRCMN*	-1.7	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23991	LYKVYKYLFPISLLKNMTPF			20	SLAY-screened peptide P2341			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTATAAGGTCTACAAGTATTTGTTTCCCATTTCGTTGTTGAAGAACATGACTCCGTTTTAA	LYKVYKYLFPISLLKNMTPF*	-1.699	0.00013	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23992	FHALWLVATGYFFNRVKMLS			20	SLAY-screened peptide P2342			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCACGCGCTCTGGCTCGTTGCCACCGGCTATTTTTTTAATAGGGTTAAGATGCTTTCTTAA	FHALWLVATGYFFNRVKMLS*	-1.699	2.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23993	RRVMYNSDTYSTRGQIKYH			19	SLAY-screened peptide P2343			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCGCGTTATGTATAATTCCGACACGTATAGCACCAGGGGTCAGATTAAGTATCACTAGTAA	RRVMYNSDTYSTRGQIKYH**	-1.699	0.002533	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23994	VVDAPRDAPRVLDI			14	SLAY-screened peptide P2344			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGTTGATGCGCCTCGGGATGCCCCGCGGGTTCTTGACATTTAGCGGCTTCTGGCTTGGTAA	VVDAPRDAPRVLDI*RLLAW*	-1.699	0.012605	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23995	PLIPWRANIPTCPCWFCHYL			20	SLAY-screened peptide P2345			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCATTCCGTGGCGTGCCAATATCCCGACGTGTCCTTGCTGGTTCTGCCACTACCTTTAA	PLIPWRANIPTCPCWFCHYL*	-1.699	0.017275	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23996	FVRDLVTSFRRDVFTRCTKG			20	SLAY-screened peptide P2346			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGTCCGGGACCTTGTCACGTCCTTTAGGCGTGATGTGTTTACTCGTTGCACCAAGGGCTAA	FVRDLVTSFRRDVFTRCTKG*	-1.698	0.017482	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23997	SVKGHCKFFLNCQRASYSPY			20	SLAY-screened peptide P2347			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGTTAAGGGTCATTGCAAGTTTTTTCTGAACTGTCAGAGGGCGAGCTATTCGCCCTACTAA	SVKGHCKFFLNCQRASYSPY*	-1.698	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23998	PPPYSLGSSAVYDSIPIYFL			20	SLAY-screened peptide P2348			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTCCTTACAGCCTGGGTTCCTCGGCTGTTTACGATAGCATCCCGATTTATTTTTTGTAA	PPPYSLGSSAVYDSIPIYFL*	-1.698	6.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP23999	TACDPYNIHAYHL			13	SLAY-screened peptide P2349			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGCGTGTGATCCGTACAATATCCACGCGTATCATCTGTAGTTTAACTAGCGTTGGTATTAA	TACDPYNIHAYHL*FN*RWY*	-1.698	0.000242	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24000	RRMQCPYCLLAISANSYTLN			20	SLAY-screened peptide P2350			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGCATGCAGTGCCCTTACTGCCTGTTGGCCATTTCCGCGAACTCCTACACTCTTAACTAA	RRMQCPYCLLAISANSYTLN*	-1.697	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24001	FIFYSV			6	SLAY-screened peptide P2351			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCATCTTTTACTCCGTTTAGTATTTTAGCCGGTTTCTTAAGATCCTTCCCCTTGATTTCTAA	FIFYSV*YFSRFLKILPLDF*	-1.697	0.000714	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24002	LFIAFLSILHIQLTFLITRR			20	SLAY-screened peptide P2352			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTTCATTGCTTTCTTGAGTATTTTGCACATTCAGCTGACCTTTTTGATTACCCGCCGTTAA	LFIAFLSILHIQLTFLITRR*	-1.697	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24003	AVNRYICRMPCRIRRPST			18	SLAY-screened peptide P2353			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGTCAACCGTTACATCTGCCGCATGCCGTGTCGGATCAGGCGGCCTTCGACTTAGACTTAC	AVNRYICRMPCRIRRPST*TY	-1.697	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24004	WPVDSTLAFLSVFFLH			16	SLAY-screened peptide P2354			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCCCGTCGATAGTACGCTCGCGTTCCTCTCGGTCTTCTTTCTGCATTAGTACGTTTTCTAA	WPVDSTLAFLSVFFLH*YVF*	-1.696	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24005	DTTPVCNMCPSPQESYCRSQ			20	SLAY-screened peptide P2355			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATACGACCCCCGTGTGTAACATGTGCCCGTCGCCGCAGGAGTCTTACTGTAGGAGTCAGTAA	DTTPVCNMCPSPQESYCRSQ*	-1.696	0.000121	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24006	DLRSGERHTRCIEDNFLITT			20	SLAY-screened peptide P2356			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCTTCGTTCCGGCGAGAGGCACACTCGCTGTATTGAGGATAATTTCCTCATTACGACCTAA	DLRSGERHTRCIEDNFLITT*	-1.696	0.000164	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24007	SEIFNNYTREKHCCPNSLTVY			21	SLAY-screened peptide P2357			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGAGATTTTCAATAATTATACTCGTGAGAAGCACTGCTGTCCGAATTCTCTCACTGTGTAC	SEIFNNYTREKHCCPNSLTVY	-1.696	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24008	MLGAVPTMQPAINTFGA			17	SLAY-screened peptide P2358			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCTCGGGGCGGTCCCCACCATGCAGCCGGCGATCAACACCTTCGGGGCCTAGTGTGTTTAA	MLGAVPTMQPAINTFGA*CV*	-1.696	0.021254	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24009	TITPRGFHRISTIGNCCRHA			20	SLAY-screened peptide P2359			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCATCACCCCTCGGGGCTTTCATAGGATCAGTACTATCGGTAACTGCTGCCGCCACGCCTAA	TITPRGFHRISTIGNCCRHA*	-1.695	0.025356	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24010	RLHDDMPKITTAYKGFCHSI			20	SLAY-screened peptide P2360			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCTTCACGACGATATGCCCAAGATCACTACTGCCTATAAGGGGTTTTGCCACTCGATTTAA	RLHDDMPKITTAYKGFCHSI*	-1.695	0.000247	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24011	KYHPDLRIVYICFSLYCSTY			20	SLAY-screened peptide P2361			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGTACCACCCGGATTTGCGTATTGTGTATATCTGTTTTTCCCTCTACTGTAGCACTTATTAA	KYHPDLRIVYICFSLYCSTY*	-1.695	0.003804	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24012	ILRRRRTRHHSYVVITLSFD			20	SLAY-screened peptide P2362			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCTGCGTCGTCGTCGGACGAGGCATCACTCGTATGTTGTCATTACTCTGTCGTTTGACTAA	ILRRRRTRHHSYVVITLSFD*	-1.695	0.001392	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24013	SYRFKAEACFLDFSEDNSFC			20	SLAY-screened peptide P2363			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTATAGGTTCAAGGCGGAGGCCTGTTTCCTTGATTTTTCCGAGGATAATTCTTTCTGTTAA	SYRFKAEACFLDFSEDNSFC*	-1.695	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24014	PIHTTYRTRRHRHSRMYRIP			20	SLAY-screened peptide P2364			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATCCACACGACTTACCGGACTCGTCGGCACCGGCACTCCCGTATGTACCGTATTCCGTAA	PIHTTYRTRRHRHSRMYRIP*	-1.694	0.007332	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24015	VTQFPESIYALPDLNHFKYI			20	SLAY-screened peptide P2365			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCACTCAGTTCCCGGAGTCTATCTATGCCCTCCCCGACCTCAACCATTTTAAGTATATTTAA	VTQFPESIYALPDLNHFKYI*	-1.694	5.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24016	RHSYGSAYSKPLVYIPPGTT			20	SLAY-screened peptide P2366			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCATTCTTATGGGAGTGCGTACTCTAAGCCTTTGGTTTATATTCCGCCGGGCACCACTTAA	RHSYGSAYSKPLVYIPPGTT*	-1.694	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24017	NYMYSYTFP			9	SLAY-screened peptide P2367			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTACATGTACAGCTACACGTTCCCATGATCTGTACCTGGTTGTGTGGTACCAAGGACTAAC	NYMYSYTFP*SVPGCVVPRTN	-1.694	0.000637	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24018	APMEGQ			6	SLAY-screened peptide P2368			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCCCATGGAGGGCCAGTAGATCCCCATCCACAACACCGTTAATCATTACAACGTCAGGTAA	APMEGQ*IPIHNTVNHYNVR*	-1.693	0.028063	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24019	SHFRAYLMLL			10	SLAY-screened peptide P2369			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCATTTTCGTGCGTATCTTATGCTGCTCTAGTTTGCTGGGTCGATGTCCGACAACCGCTAA	SHFRAYLMLL*FAGSMSDNR*	-1.693	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24020	GLRTTHEVDHALSSHISGHH			20	SLAY-screened peptide P2370			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGTTGCGCACCACGCACGAGGTCGACCATGCGCTTAGCTCGCATATCTCTGGTCATCACTAA	GLRTTHEVDHALSSHISGHH*	-1.693	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24021	QDAIDHPFHAYRSKINTYLM			20	SLAY-screened peptide P2371			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGATGCTATCGACCATCCGTTCCATGCCTACCGTAGTAAGATTAACACTTATCTGATGTAA	QDAIDHPFHAYRSKINTYLM*	-1.693	0.040637	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24022	SQQATIRVDYLRFLYIQHYL			20	SLAY-screened peptide P2372			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCAGCAGGCGACTATCCGCGTGGACTATCTCAGGTTCCTTTATATTCAGCATTATCTGTAA	SQQATIRVDYLRFLYIQHYL*	-1.693	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24023	NIFYDRDNSCPRETTHFDYR			20	SLAY-screened peptide P2373			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATATCTTTTACGATCGCGATAACTCTTGTCCCCGGGAGACCACTCACTTTGACTACCGTTAA	NIFYDRDNSCPRETTHFDYR*	-1.693	0.00156	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24024	LHVYYPFLL			9	SLAY-screened peptide P2374			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCACGTGTACTACCCTTTTCTCCTGTAGACGGTTAATCCCTGTCCTTGCGAGTAGCACTAA	LHVYYPFLL*TVNPCPCE*H*	-1.693	0.001921	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24025	AHLYPTALYN			10	SLAY-screened peptide P2375			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCATCTTTACCCGACTGCGCTGTATAATTAGGTGACTGCCCCTATCTTGGCTCCGCGTTAA	AHLYPTALYN*VTAPILAPR*	-1.693	0.018557	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24026	DSFMDAWSGNYFLTPVRRSRN			21	SLAY-screened peptide P2376			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTCTTTTATGGATGCTTGGTCGGGTAACTATTTTTTGACACCAGTTCGCAGGTCTCGTAAC	DSFMDAWSGNYFLTPVRRSRN	-1.693	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24027	SANRIRIHFLMITNITHQDD			20	SLAY-screened peptide P2377			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCTAACCGCATCAGGATCCATTTTCTTATGATCACTAACATCACTCACCAGGATGACTAA	SANRIRIHFLMITNITHQDD*	-1.692	0.000517	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24028	HPHYYEYCSTATSPTNNIGS			20	SLAY-screened peptide P2378			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCCCATTATTATGAGTACTGCTCCACGGCCACCAGTCCCACTAATAATATCGGTAGCTAA	HPHYYEYCSTATSPTNNIGS*	-1.692	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24029	PALIQSAQHVLSDTFCLLCC			20	SLAY-screened peptide P2379			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCCCTTATCCAGAGTGCTCAGCATGTTCTGTCTGATACCTTTTGCCTCCTCTGTTGTTAA	PALIQSAQHVLSDTFCLLCC*	-1.692	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24030	SL			2	SLAY-screened peptide P2380			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTTGTAGTTTTCGTGTCTTGACCGTTGTATTGATCTTCACATTTGTGTTCATTATACCTAA	SL*FSCLDRCIDLHICVHYT*	-1.692	0.001525	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24031	IPDVLLDSWNRYGRFIL			17	SLAY-screened peptide P2381			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCCTGACGTCCTCCTGGACTCCTGGAACAGGTACGGAAGGTTCATACTTTGACGCTCTAAC	IPDVLLDSWNRYGRFIL*RSN	-1.692	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24032	VPNAYPSQRSYHPPSLSSIT			20	SLAY-screened peptide P2382			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCCCAACGCCTATCCGAGTCAGAGGTCTTACCATCCCCCTAGCCTTAGCTCGATTACCTAA	VPNAYPSQRSYHPPSLSSIT*	-1.692	0.002021	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24033	PDVLD			5	SLAY-screened peptide P2383			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGATGTGCTGGACTAGCCGGTCGTCAGCCGTCTCATCTACAGGGACCGTCCGGACAACTAA	PDVLD*PVVSRLIYRDRPDN*	-1.691	0.020668	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24034	RRAGAQITLSVLFYPGCALV			20	SLAY-screened peptide P2384			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCGCGCCGGGGCTCAGATTACCCTTAGCGTGCTTTTTTATCCCGGCTGCGCTTTGGTTTAA	RRAGAQITLSVLFYPGCALV*	-1.69	6.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24035	LPSRNPYGWSTVAGPLIHII			20	SLAY-screened peptide P2385			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCCAGCCGGAATCCCTACGGTTGGTCCACTGTTGCTGGCCCTCTTATTCATATTATTTAA	LPSRNPYGWSTVAGPLIHII*	-1.69	0.023234	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24036	NACCVFAPAPCADRGALGCN			20	SLAY-screened peptide P2386			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGCCTGCTGTGTCTTTGCCCCGGCCCCCTGTGCTGACCGTGGTGCTTTGGGGTGTAATTAA	NACCVFAPAPCADRGALGCN*	-1.69	0.015092	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24037	VNSVTIVLVFSLFFRRGN			18	SLAY-screened peptide P2387			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTAATTCCGTAACAATCGTACTTGTCTTCTCATTGTTTTTTAGACGGGGTAACTGAGTAAGT	VNSVTIVLVFSLFFRRGN*VS	-1.69	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24038	ALSASIGDFSRNPLKFVSNN			20	SLAY-screened peptide P2388			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCTCTCTGCCTCGATCGGTGACTTTTCGAGGAACCCTTTGAAGTTCGTCTCGAACAATTAA	ALSASIGDFSRNPLKFVSNN*	-1.69	0.001836	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24039	PLTCPLSCCLWAWAPRRSLV			20	SLAY-screened peptide P2389			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTACCTGCCCGCTCAGCTGCTGCCTCTGGGCTTGGGCCCCTCGTAGGAGTCTGGTTTAA	PLTCPLSCCLWAWAPRRSLV*	-1.69	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24040	YAIYSLDFNDGFSHVTPDWS			20	SLAY-screened peptide P2390			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGCTATCTATTCGCTGGATTTTAATGACGGGTTCAGTCACGTTACTCCCGATTGGTCTTAA	YAIYSLDFNDGFSHVTPDWS*	-1.69	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24041	QALPAPNALTRATVIGVHFI			20	SLAY-screened peptide P2391			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGCGCTGCCCGCTCCCAACGCGCTTACGCGCGCGACTGTGATTGGCGTGCACTTCATTTAA	QALPAPNALTRATVIGVHFI*	-1.69	0.001147	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24042	VRVKAHINRLFLTYKHVYAD			20	SLAY-screened peptide P2392			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCAGGGTTAAGGCGCACATTAACCGTCTTTTTCTGACTTATAAGCATGTTTATGCCGACTAA	VRVKAHINRLFLTYKHVYAD*	-1.689	0.008145	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24043	CA			2	SLAY-screened peptide P2393			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCGTAGAGCCAGACTGGAATTTGTCGCGCACGGGGCCTTATGGTAAGTAGCATAAGTAAC	CA*SQTGICRARGLMVSSISN	-1.689	0.00087	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24044	SHWNNFHFTFTCEVGHPKLC			20	SLAY-screened peptide P2394			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCACTGGAATAATTTTCACTTCACGTTTACCTGTGAGGTTGGGCACCCTAAGCTGTGTTAA	SHWNNFHFTFTCEVGHPKLC*	-1.689	0.017499	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24045	ELPLVHALVFWLRITQSPVV			20	SLAY-screened peptide P2395			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCTGCCTCTGGTCCACGCCCTGGTTTTTTGGCTGCGTATCACTCAGTCGCCCGTGGTTTAA	ELPLVHALVFWLRITQSPVV*	-1.689	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24046	VYPHPTPGHATNCDAPCRFV			20	SLAY-screened peptide P2396			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTATCCTCACCCTACCCCCGGGCATGCTACCAATTGTGACGCGCCTTGTCGGTTCGTGTAA	VYPHPTPGHATNCDAPCRFV*	-1.688	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24047	AMTRILTISTSTVSG			15	SLAY-screened peptide P2397			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGATGACGCGTATTTTAACAATATCCACATCAACTGTAAGTGGCTGATTATTTATTATTAAC	AMTRILTISTSTVSG*LFIIN	-1.688	0.0022	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24048	SYQPIIRITSIPYTLSL			17	SLAY-screened peptide P2398			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTATCAGCCCATTATCCGCATTACGTCCATCCCCTACACCCTTTCCCTCTAGTGTCGTTAA	SYQPIIRITSIPYTLSL*CR*	-1.688	0.001819	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24049	NQTVGGPSRHHTAELLRIVD			20	SLAY-screened peptide P2399			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCAGACTGTGGGCGGCCCGTCCCGTCATCACACCGCTGAGCTCCTCAGGATCGTGGACTAA	NQTVGGPSRHHTAELLRIVD*	-1.688	0.046602	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24050	PCTPLNNSSRLYDLAPSSTA			20	SLAY-screened peptide P2400			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGTACGCCGCTTAATAACAGTAGCCGTCTGTACGATTTGGCTCCCAGCAGTACCGCGTAA	PCTPLNNSSRLYDLAPSSTA*	-1.688	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24051	SHASTCFMYWLSKFTTTLIR			20	SLAY-screened peptide P2401			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCACGCCTCGACTTGCTTTATGTACTGGCTGAGTAAGTTTACTACGACTCTTATTCGCTAA	SHASTCFMYWLSKFTTTLIR*	-1.688	0.007768	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24052	HPSSEAVYSSLCLITPATGS			20	SLAY-screened peptide P2402			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCCAGCTCGGAGGCCGTTTATTCTTCCCTCTGTCTTATTACCCCCGCGACCGGCTCTTAA	HPSSEAVYSSLCLITPATGS*	-1.687	0.000186	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24053	GDHFTHAPATSQHICPMFAD			20	SLAY-screened peptide P2403			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTGACCATTTTACTCACGCCCCGGCGACCAGCCAGCATATCTGTCCTATGTTTGCTGACTAA	GDHFTHAPATSQHICPMFAD*	-1.687	0.024453	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24054	HPLSPNREPFCPHATWYKLP			20	SLAY-screened peptide P2404			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCTCTGAGTCCCAACCGTGAGCCTTTTTGTCCGCACGCTACGTGGTACAAGCTCCCCTAA	HPLSPNREPFCPHATWYKLP*	-1.687	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24055	PLNLSLHISSDPHICIDHVP			20	SLAY-screened peptide P2405			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTCAACTTGTCCCTGCATATTAGTAGTGACCCGCACATTTGCATCGATCATGTTCCTTAA	PLNLSLHISSDPHICIDHVP*	-1.686	0.000357	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24056	DHGVVVVSFCRYPTCPANRT			20	SLAY-screened peptide P2406			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCACGGCGTGGTTGTCGTGTCCTTCTGTAGGTATCCTACTTGCCCTGCTAATCGTACCTAA	DHGVVVVSFCRYPTCPANRT*	-1.686	0.003789	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24057	RHAHMQTSTSPHH			13	SLAY-screened peptide P2407			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCACGCCCATATGCAGACCTCCACTAGCCCCCATCATTAGTGTCCTTTTTATGTCGGGTAA	RHAHMQTSTSPHH*CPFYVG*	-1.686	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24058	GLGVIYHMPGRCALLSCGAT			20	SLAY-screened peptide P2408			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCTGGGTGTTATCTACCATATGCCTGGCCGCTGCGCGCTTCTCTCGTGCGGGGCCACTTAA	GLGVIYHMPGRCALLSCGAT*	-1.686	0.006992	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24059	PRITAHDAMHS			11	SLAY-screened peptide P2409			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGCATCACCGCGCACGATGCCATGCATAGCTAGCCCCCTATCATTTCTTTCGGCATTTAA	PRITAHDAMHS*PPIISFGI*	-1.686	3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24060	HNGGLPVFDRRFGPLPRYYA			20	SLAY-screened peptide P2410			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAACGGCGGGTTGCCGGTTTTCGACCGCAGGTTCGGCCCGCTGCCGCGTTATTATGCTTAA	HNGGLPVFDRRFGPLPRYYA*	-1.685	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24061	LNSRRDTIHVSFYVKYIHPP			20	SLAY-screened peptide P2411			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAACAGTCGGCGTGATACCATTCATGTCAGTTTTTACGTCAAGTACATCCATCCCCCGTAA	LNSRRDTIHVSFYVKYIHPP*	-1.685	0.003698	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24062	PYLTP			5	SLAY-screened peptide P2412			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTACCTTACGCCGTAGCTCTTTCATCTGATCAATTTGGTGGACTATAGCACGTTCAACTAA	PYLTP*LFHLINLVDYSTFN*	-1.685	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24063	ERWLSITLLTLTGPPGTIRAN			21	SLAY-screened peptide P2413			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAACGATGGCTATCCATCACTCTACTGACACTGACTGGGCCTCCCGGTACTATCAGAGCTAAC	ERWLSITLLTLTGPPGTIRAN	-1.685	0.001618	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24064	YYLSPSLTPVR			11	SLAY-screened peptide P2414			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTACTTGAGTCCCTCGCTCACTCCGGTTCGTTAGATTTATAGGGCTATGCACTCTTATTAA	YYLSPSLTPVR*IYRAMHSY*	-1.685	0.005885	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24065	LLPPMYGPYSDASVLPAKTP			20	SLAY-screened peptide P2415			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCTGCCCCCTATGTATGGTCCCTACTCGGACGCGAGCGTGTTGCCGGCTAAGACGCCCTAA	LLPPMYGPYSDASVLPAKTP*	-1.685	1.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24066	VLLTHAATFPESDVHVRLPN			20	SLAY-screened peptide P2416			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCTGCTTACCCACGCTGCCACGTTCCCGGAGAGCGATGTTCACGTGCGCCTGCCGAACTAA	VLLTHAATFPESDVHVRLPN*	-1.684	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24067	SSKHDRCSIRVVCFTDCNNY			20	SLAY-screened peptide P2417			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTCCAAGCACGATCGCTGCTCTATCCGCGTGGTCTGCTTCACGGATTGTAACAACTATTAA	SSKHDRCSIRVVCFTDCNNY*	-1.684	0.002402	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24068	GPWAIYKNGGDVPMVTSAPS			20	SLAY-screened peptide P2418			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCCTTGGGCCATTTATAAGAACGGCGGTGATGTCCCCATGGTGACTTCCGCTCCCTCCTAA	GPWAIYKNGGDVPMVTSAPS*	-1.684	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24069	RSTHYNMTLS			10	SLAY-screened peptide P2419			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCGACGCACTACAACATGACTCTGTCCTAGACGGTTTATCCCCGTAAGTCTCCCTCTTAA	RSTHYNMTLS*TVYPRKSPS*	-1.684	0.000837	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24070	INVHVFFLSGKKSSRRDYDS			20	SLAY-screened peptide P2420			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAACGTTCACGTTTTTTTCCTTTCTGGTAAGAAGAGCTCTAGGCGCGATTATGACTCTTAA	INVHVFFLSGKKSSRRDYDS*	-1.684	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24071	YAPYKYSMVRAYNNLSASKK			20	SLAY-screened peptide P2421			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGCCCCTTACAAGTACTCCATGGTTCGCGCGTATAATAACTTGTCCGCCTCGAAGAAGTAA	YAPYKYSMVRAYNNLSASKK*	-1.683	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24072	RFSRNHRSDFRKTAPSSTQY			20	SLAY-screened peptide P2422			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTTCTCTCGTAATCATCGCTCTGATTTTCGCAAGACTGCGCCCTCCAGTACTCAGTACTAA	RFSRNHRSDFRKTAPSSTQY*	-1.682	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24073	HNTPFHFPAGESTYRNYPDA			20	SLAY-screened peptide P2423			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAACACCCCCTTCCACTTTCCTGCGGGCGAGAGCACCTATCGTAACTATCCTGACGCGTAA	HNTPFHFPAGESTYRNYPDA*	-1.682	0.017059	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24074	RTGCINVTRTASTTSSLNVK			20	SLAY-screened peptide P2424			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGACGGGGTGTATTAATGTCACCCGGACTGCGTCCACTACGAGCAGCCTGAATGTCAAGTAA	RTGCINVTRTASTTSSLNVK*	-1.682	0.004405	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24075	TPSLTMTLYPMRKRILSRTP			20	SLAY-screened peptide P2425			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCCCAGCCTGACCATGACGCTGTATCCCATGCGGAAGCGCATCCTCTCTCGTACGCCGTAA	TPSLTMTLYPMRKRILSRTP*	-1.682	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24076	TGGSVHTMLHDPNYIGCANL			20	SLAY-screened peptide P2426			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGGCGGCAGTGTCCACACGATGCTCCACGATCCCAACTACATTGGCTGCGCGAACCTGTAA	TGGSVHTMLHDPNYIGCANL*	-1.682	0.00173	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24077	CVFFALLETNPNGDHDPSSI			20	SLAY-screened peptide P2427			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGTCTTCTTCGCTCTCCTCGAGACTAATCCGAATGGTGACCACGACCCGTCTTCGATCTAA	CVFFALLETNPNGDHDPSSI*	-1.682	0.016679	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24078	PGIPIDSCYNCYFDIRPGST			20	SLAY-screened peptide P2428			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGGGATTCCCATTGATAGCTGTTATAACTGCTATTTTGATATCCGGCCGGGTAGTACCTAA	PGIPIDSCYNCYFDIRPGST*	-1.682	0.000179	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24079	GRGIHRVPCTALSDPGYLVA			20	SLAY-screened peptide P2429			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCGCGGCATCCATCGCGTTCCGTGTACCGCCTTGTCCGACCCTGGCTATCTCGTCGCTTAA	GRGIHRVPCTALSDPGYLVA*	-1.681	0.018335	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24080	DQDYGFLHDASSFYNHNYRP			20	SLAY-screened peptide P2430			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCAGGACTACGGTTTCCTCCACGACGCTTCGTCTTTTTACAACCACAACTACAGGCCTTAA	DQDYGFLHDASSFYNHNYRP*	-1.681	2.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24081	MVHLNNILEPASNTSHMAVN			20	SLAY-screened peptide P2431			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGTGCATCTGAACAACATTCTCGAGCCGGCCTCTAACACTAGTCATATGGCCGTTAATTAA	MVHLNNILEPASNTSHMAVN*	-1.681	2.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24082	YPWSQSKPDS			10	SLAY-screened peptide P2432			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCCGTGGTCGCAGTCGAAGCCGGACAGCTAGAAGAATCCGAAGTATATGCGCCTCAGTTAA	YPWSQSKPDS*KNPKYMRLS*	-1.681	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24083	CDWPISTPQDNNIRCQRILCN			21	SLAY-screened peptide P2433			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGACTGGCCCATTAGTACCCCTCAGGATAACAATATTCGTTGTCAAAGAATATTATGTAAC	CDWPISTPQDNNIRCQRILCN	-1.68	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24084	HAMPDATILPSVTDYTTSYS			20	SLAY-screened peptide P2434			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGCTATGCCTGATGCTACTATTCTCCCTTCTGTGACGGATTACACTACGTCGTATAGCTAA	HAMPDATILPSVTDYTTSYS*	-1.68	0.026763	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24085	SILRHCSCNCLDQLIT			16	SLAY-screened peptide P2435			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGATCCTGCGCCACTGCAGCTGCAACTGTCTTGATCAGCTCATTACTTAGGACGTCACCTAA	SILRHCSCNCLDQLIT*DVT*	-1.68	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24086	SSCSVSTTAYLSYPPNRHIH			20	SLAY-screened peptide P2436			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAGCTGTAGTGTCAGTACGACCGCCTACCTGTCTTATCCGCCTAATAGGCATATCCATTAA	SSCSVSTTAYLSYPPNRHIH*	-1.68	0.001013	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24087	VCVPQAPEYRVFPLYNT			17	SLAY-screened peptide P2437			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTGTGTGCCTCAGGCTCCCGAGTATCGCGTGTTTCCGCTTTATAACACTTAGCTGGCGTAA	VCVPQAPEYRVFPLYNT*LA*	-1.68	0.007508	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24088	LRQTPPHFYFNLCYPHWRPP			20	SLAY-screened peptide P2438			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCGCCAGACGCCGCCCCACTTCTATTTCAACCTCTGCTATCCTCATTGGAGGCCGCCGTAA	LRQTPPHFYFNLCYPHWRPP*	-1.679	0.000164	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24089	SVTFS			5	SLAY-screened peptide P2439			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGTGACTTTCTCCTAGGTTGGCTCCCCCGCGATCCATCGCTGGTTTCTCATCTCGAGTTAA	SVTFS*VGSPAIHRWFLISS*	-1.679	8.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24090	FPLFHNIHPTNLPVPREDFE			20	SLAY-screened peptide P2440			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCCCCTGTTTCATAACATTCATCCCACTAACCTTCCGGTGCCTCGGGAGGACTTTGAGTAA	FPLFHNIHPTNLPVPREDFE*	-1.679	3.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24091	PLSCPSHEPFICLRTLRFVR			20	SLAY-screened peptide P2441			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTGTCGTGCCCTTCGCACGAGCCCTTTATTTGCCTCCGCACGCTCCGCTTTGTTAGGTAA	PLSCPSHEPFICLRTLRFVR*	-1.679	0.004667	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24092	TSDSSST			7	SLAY-screened peptide P2442			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAGTGACTCTTCCTCGACCTAGCGGTTCAGTTGCATCTGTCTTGAGACTCGCCCGTCGTAA	TSDSSST*RFSCICLETRPS*	-1.678	0.008806	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24093	VNLT			4	SLAY-screened peptide P2443			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTAACCTTACTTAGCACCACACGCCTTTTCAGCACATTTACTATTCTTTTTCGTAGCATTAA	VNLT*HHTPFQHIYYSFS*H*	-1.678	0.008873	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24094	LPPIRDWRSVIVLPLHIATI			20	SLAY-screened peptide P2444			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCCCCCATCCGCGACTGGCGTTCCGTCATTGTCCTGCCTCTTCATATTGCGACGATCTAA	LPPIRDWRSVIVLPLHIATI*	-1.678	0.001827	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24095	PGYFPWNHRSPRLTESDTAQ			20	SLAY-screened peptide P2445			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGTTATTTTCCCTGGAACCATAGGTCGCCCCGTCTGACGGAGTCCGATACTGCTCAGTAA	PGYFPWNHRSPRLTESDTAQ*	-1.678	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24096	CNCCVHSPTYYPALLWAN			18	SLAY-screened peptide P2446			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAATTGCTGCGTTCATAGTCCCACCTATTATCCCGCTCTCCTTTGGGCGAACTAGACCTAA	CNCCVHSPTYYPALLWAN*T*	-1.678	0.02565	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24097	NNQDATYGSVRWLPVDSASL			20	SLAY-screened peptide P2447			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACAACCAGGATGCCACTTATGGCAGTGTTCGCTGGCTTCCTGTTGACTCGGCCTCGCTTTAA	NNQDATYGSVRWLPVDSASL*	-1.677	0.001445	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24098	VDLRVAQSKPLYRSSLMLHR			20	SLAY-screened peptide P2448			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGGATTTGCGCGTGGCTCAGTCTAAGCCGCTGTACAGGTCTTCGCTTATGTTGCATAGGTAA	VDLRVAQSKPLYRSSLMLHR*	-1.677	0.022009	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24099	SAGLSFRIVSFFHFRVRLRFN			21	SLAY-screened peptide P2449			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCTGGTCTGTCCTTTAGGATCGTTAGCTTTTTCCATTTTAGGGTTAGGCTAAGGTTTAAC	SAGLSFRIVSFFHFRVRLRFN	-1.676	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24100	AALKCLLSLPVYPQRAADSF			20	SLAY-screened peptide P2450			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGCCCTTAAGTGTCTCCTTAGTCTGCCGGTCTATCCGCAGAGGGCCGCTGACTCCTTCTAA	AALKCLLSLPVYPQRAADSF*	-1.676	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24101	RMCRKFKTWTHLHLRGRRTR			20	SLAY-screened peptide P2451			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATGTGCCGTAAGTTTAAGACCTGGACGCATCTGCATCTCAGGGGCCGCCGGACCCGTTAA	RMCRKFKTWTHLHLRGRRTR*	-1.676	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24102	PSWT			4	SLAY-screened peptide P2452			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCGTGGACCTAGCGCCCCCGGTATTCTTGTACTAACCTGTTCTACTATCCCTGCTTTTAA	PSWT*RPRYSCTNLFYYPCF*	-1.676	0.00078	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24103	LLCMLCGPTPFHHCPASSLH			20	SLAY-screened peptide P2453			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTGTGTATGCTGTGCGGCCCTACTCCTTTTCATCATTGCCCTGCCAGTTCGCTGCATTAA	LLCMLCGPTPFHHCPASSLH*	-1.675	3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24104	FSSHTPAPCMPPPNWRFAWV			20	SLAY-screened peptide P2454			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTAGTTCTCATACGCCGGCGCCCTGTATGCCCCCTCCGAACTGGAGGTTCGCGTGGGTGTAA	FSSHTPAPCMPPPNWRFAWV*	-1.674	0.001981	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24105	GPVSDSSGPFSHCIILAAPV			20	SLAY-screened peptide P2455			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCCGGTCTCTGACAGTAGTGGGCCGTTCAGCCATTGCATCATCCTGGCTGCTCCTGTTTAA	GPVSDSSGPFSHCIILAAPV*	-1.674	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24106	LSPLPRHNISQTLRPPFLV			19	SLAY-screened peptide P2456			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCCCCTTTGCCTCGGCATAATATCTCTCAGACGCTGCGCCCCCCCTTCCTGGTCTAGTAA	LSPLPRHNISQTLRPPFLV**	-1.674	0.006374	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24107	PVFIYSLKPMVPKKPNTRVL			20	SLAY-screened peptide P2457			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGTGTTTATCTACTCCCTCAAGCCTATGGTCCCGAAGAAGCCTAACACGCGTGTTTTGTAA	PVFIYSLKPMVPKKPNTRVL*	-1.673	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24108	RCACNRRRYHPRNPDDYLPM			20	SLAY-screened peptide P2458			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGCGCCTGTAACCGCCGGCGCTATCATCCTAGGAATCCTGATGACTACCTGCCCATGTAA	RCACNRRRYHPRNPDDYLPM*	-1.673	0.000544	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24109	PLEVLPCFRWPNFLPYILAA			20	SLAY-screened peptide P2459			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCGAGGTGCTGCCCTGCTTTAGGTGGCCTAACTTTCTCCCTTACATTCTGGCTGCGTAA	PLEVLPCFRWPNFLPYILAA*	-1.673	0.025664	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24110	TAAFGPFLACPLGINSYTFT			20	SLAY-screened peptide P2460			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGCTGCTTTCGGGCCCTTTTTGGCCTGTCCGCTTGGCATTAACAGCTATACGTTCACGTAA	TAAFGPFLACPLGINSYTFT*	-1.673	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24111	VNRYARFYHSTVYIRIPCMI			20	SLAY-screened peptide P2461			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCAATCGGTACGCCCGTTTCTATCATTCCACTGTGTACATTCGCATTCCCTGTATGATCTAA	VNRYARFYHSTVYIRIPCMI*	-1.673	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24112	CVPDKSVLTLIGYTHD			16	SLAY-screened peptide P2462			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGTGCCGGATAAGTCCGTTCTCACGCTCATCGGTTATACGCATGACTAGCCCTCCACCTAA	CVPDKSVLTLIGYTHD*PST*	-1.673	0.003049	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24113	TPHYFRNQPLFHQTHITYLA			20	SLAY-screened peptide P2463			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCCGCATTATTTTCGTAATCAGCCCTTGTTCCATCAGACCCATATCACTTATCTTGCGTAA	TPHYFRNQPLFHQTHITYLA*	-1.673	0.008353	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24114	IVNGLFVQTPTTLPILHTAS			20	SLAY-screened peptide P2464			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGTTAACGGGCTGTTTGTTCAGACTCCGACCACTCTCCCCATTTTGCACACTGCTAGCTAA	IVNGLFVQTPTTLPILHTAS*	-1.673	0.000102	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24115	THLRAPPCMGDFIWFVLFSF			20	SLAY-screened peptide P2465			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCACCTCCGTGCGCCCCCCTGCATGGGGGATTTCATTTGGTTTGTTCTCTTTTCCTTCTAA	THLRAPPCMGDFIWFVLFSF*	-1.673	0.013461	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24116	TANQPIAFFPRLMLTSRLVPN			21	SLAY-screened peptide P2466			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGCCAACCAGCCTATCGCCTTCTTTCCCCGTCTTATGCTCACAAGTCGACTCGTTCCTAAC	TANQPIAFFPRLMLTSRLVPN	-1.672	0.001371	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24117	ASYRPSCSSVLYDLKPAQGG			20	SLAY-screened peptide P2467			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTCTTATCGCCCGTCGTGTTCTAGCGTCTTGTACGACCTCAAGCCCGCCCAGGGTGGTTAA	ASYRPSCSSVLYDLKPAQGG*	-1.672	0.023835	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24118	THSPLVSFIMPPPLTPLCLV			20	SLAY-screened peptide P2468			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCATTCCCCGCTGGTCTCCTTCATCATGCCGCCGCCGCTTACTCCCCTCTGTTTGGTCTAA	THSPLVSFIMPPPLTPLCLV*	-1.672	0.000649	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24119	SMPPSRINYVGLHLDKRMLA			20	SLAY-screened peptide P2469			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCATGCCGCCTTCGCGTATCAACTACGTCGGCTTGCACCTTGACAAGCGGATGCTCGCGTAA	SMPPSRINYVGLHLDKRMLA*	-1.671	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24120	NKNISSLLQHR			11	SLAY-screened peptide P2470			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACAAGAATATTTCTTCCCTCTTGCAGCATCGGTAGGGTCCTTCCAGCGATGCTCGGCCTTAA	NKNISSLLQHR*GPSSDARP*	-1.671	0.014222	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24121	GLLSPWSVRFNYHDPDADHL			20	SLAY-screened peptide P2471			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCTGCTCTCTCCGTGGTCTGTGCGCTTCAATTACCATGACCCGGACGCTGATCACTTGTAA	GLLSPWSVRFNYHDPDADHL*	-1.671	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24122	SMNLNCPMVSCSHRDCLVVI			20	SLAY-screened peptide P2472			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTATGAACCTTAACTGCCCGATGGTTTCCTGTTCTCACCGTGACTGCCTCGTGGTTATTTAA	SMNLNCPMVSCSHRDCLVVI*	-1.67	0.000474	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24123	LIAARLFDCPLRQDSHCTWI			20	SLAY-screened peptide P2473			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATCGCGGCGCGCCTCTTTGACTGTCCTCTTCGCCAGGATTCGCATTGTACCTGGATTTAA	LIAARLFDCPLRQDSHCTWI*	-1.67	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24124	VLVIASSYYF			10	SLAY-screened peptide P2474			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCTCGTTATTGCCAGCTCCTATTACTTCTAGCATCTGCATATCTATCTTGACCCGTCTTAA	VLVIASSYYF*HLHIYLDPS*	-1.67	1.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24125	RDRLQIIPTYGLSTVPLRGI			20	SLAY-screened peptide P2475			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGACCGGCTCCAGATCATCCCTACTTACGGCCTCAGTACCGTTCCCCTCCGTGGCATTTAA	RDRLQIIPTYGLSTVPLRGI*	-1.669	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24126	IQMGIHRLTITNNYFTTFLY			20	SLAY-screened peptide P2476			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCAGATGGGTATTCACCGTTTGACGATTACCAATAACTATTTTACTACGTTTTTGTATTAA	IQMGIHRLTITNNYFTTFLY*	-1.668	0.002182	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24127	LENAAYTDYLDLPEHPCNST			20	SLAY-screened peptide P2477			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGAGAACGCCGCCTATACCGACTACCTTGATCTTCCCGAGCATCCCTGTAATAGCACCTAA	LENAAYTDYLDLPEHPCNST*	-1.668	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24128	YFRVPPTHLMLTPLVLYTLA			20	SLAY-screened peptide P2478			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTTCCGGGTCCCTCCCACCCACCTGATGCTGACTCCCTTGGTCCTCTACACTCTGGCGTAA	YFRVPPTHLMLTPLVLYTLA*	-1.668	0.021252	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24129	RSHSARLPALILPAPASLVIN			21	SLAY-screened peptide P2479			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCGCACTCCGCGAGGCTCCCTGCTCTGATCTTACCCGCGCCTGCCTCCTTGGTGATTAAC	RSHSARLPALILPAPASLVIN	-1.668	0.004286	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24130	PTNSVHAYGIF			11	SLAY-screened peptide P2480			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACTAACTCGGTTCATGCGTATGGCATTTTCTAGGGCGTGATGCACCTGGTGACTGTCTAA	PTNSVHAYGIF*GVMHLVTV*	-1.668	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24131	RFTLNCFLGTRIRRKIGCSR			20	SLAY-screened peptide P2481			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTTCACTTTGAACTGTTTTCTCGGCACTCGCATCCGTCGCAAGATTGGGTGTAGCCGCTAA	RFTLNCFLGTRIRRKIGCSR*	-1.667	0.002099	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24132	LCGGSGPDCSDQSDNLANTI			20	SLAY-screened peptide P2482			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTGCGGTGGCAGCGGTCCGGATTGCAGTGATCAGAGCGATAACCTGGCTAACACGATTTAA	LCGGSGPDCSDQSDNLANTI*	-1.667	0.000419	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24133	CWYIDRPALSRFRPVRTLLA			20	SLAY-screened peptide P2483			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTGGTATATCGATCGTCCGGCTCTCTCGCGCTTCCGGCCGGTTAGGACCCTGCTTGCTTAA	CWYIDRPALSRFRPVRTLLA*	-1.666	0.014851	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24134	SSPAAPRGRSPQSSRVG			17	SLAY-screened peptide P2484			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAGTCCGGCTGCGCCGCGCGGTCGCTCCCCTCAGAGTAGCCGCGTTGGTTAGAATGCGTAA	SSPAAPRGRSPQSSRVG*NA*	-1.666	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24135	CTTTALANFTLSRPKFDKIS			20	SLAY-screened peptide P2485			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCACCACTACCGCCCTCGCGAATTTTACTTTGAGTAGGCCTAAGTTTGATAAGATCTCTTAA	CTTTALANFTLSRPKFDKIS*	-1.666	0.002975	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24136	CFHYDYCCSCQSHPHWLLFA			20	SLAY-screened peptide P2486			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTTCCACTACGATTACTGCTGCTCTTGTCAGTCCCACCCCCACTGGCTCCTCTTTGCTTAA	CFHYDYCCSCQSHPHWLLFA*	-1.666	0.000157	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24137	LPKTSLRFRANAGPTPHPST			20	SLAY-screened peptide P2487			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCCAAGACCAGCCTCCGCTTTCGGGCCAATGCTGGTCCCACTCCCCACCCGTCGACCTAA	LPKTSLRFRANAGPTPHPST*	-1.666	0.017517	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24138	LSYVSPGAGYSPASDTPTRA			20	SLAY-screened peptide P2488			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCCTACGTCTCTCCCGGGGCTGGGTACTCCCCGGCTAGCGACACGCCTACCCGGGCTTAA	LSYVSPGAGYSPASDTPTRA*	-1.666	0.003337	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24139	SLAIILLSYPDFSMHSILAF			20	SLAY-screened peptide P2489			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTTGGCCATCATCCTGTTGTCCTATCCGGACTTCTCCATGCATTCCATTCTTGCCTTTTAA	SLAIILLSYPDFSMHSILAF*	-1.665	1.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24140	PSSRR			5	SLAY-screened peptide P2490			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGCTCGCGGCGGTAGACTATGTAGATCAACCACACCTTGAACTGTAATCGCACTCATTAA	PSSRR*TM*INHTLNCNRTH*	-1.665	0.046519	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24141	AAVTAIRQLFNCLIMTARCS			20	SLAY-screened peptide P2491			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGCCGTCACCGCCATCAGGCAGCTTTTCAATTGTCTTATTATGACCGCCCGCTGTTCCTAA	AAVTAIRQLFNCLIMTARCS*	-1.665	0.004576	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24142	SQTRPKHSLVMPFSYTLTMP			20	SLAY-screened peptide P2492			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCAGACTCGTCCGAAGCATAGTCTCGTTATGCCGTTTAGTTATACGTTGACCATGCCGTAA	SQTRPKHSLVMPFSYTLTMP*	-1.665	0.000224	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24143	CIKCYSVYCLLFNYRHTGVT			20	SLAY-screened peptide P2493			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTATCAAGTGTTATTCTGTGTATTGCCTGCTGTTCAATTACCGTCATACCGGTGTCACGTAA	CIKCYSVYCLLFNYRHTGVT*	-1.665	0.014298	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24144	LANPCNIYNHAPMYQSLRYA			20	SLAY-screened peptide P2494			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGCCAATCCTTGCAACATCTACAATCATGCGCCCATGTATCAGAGTCTTCGTTACGCCTAA	LANPCNIYNHAPMYQSLRYA*	-1.665	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24145	VVSVRVVREYTLADGFIMSC			20	SLAY-screened peptide P2495			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGGTTAGCGTCCGTGTGGTCCGTGAGTATACCCTTGCTGATGGTTTTATCATGTCCTGTTAA	VVSVRVVREYTLADGFIMSC*	-1.665	0.000188	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24146	HRHLCSQERHTRSARVDRMS			20	SLAY-screened peptide P2496			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCGGCACCTGTGCTCCCAGGAGCGTCATACTCGCTCCGCGCGCGTCGACCGCATGTCCTAA	HRHLCSQERHTRSARVDRMS*	-1.665	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24147	MVHSSDSTWFSFPGELHDSP			20	SLAY-screened peptide P2497			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGTGCACTCTTCTGACTCCACTTGGTTTTCGTTCCCCGGTGAGTTGCATGATAGCCCGTAA	MVHSSDSTWFSFPGELHDSP*	-1.664	7.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24148	SPAPLLCRVRPRSRRTTASSN			21	SLAY-screened peptide P2498			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCCTGCCCCCTTACTGTGCAGGGTCCGACCTAGAAGCAGACGGACAACAGCGTCTTCTAAC	SPAPLLCRVRPRSRRTTASSN	-1.664	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24149	PYHRPFSPPNYDQSNGHSGT			20	SLAY-screened peptide P2499			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTACCACCGTCCGTTTTCTCCCCCTAATTATGATCAGAGCAATGGTCACTCTGGGACCTAA	PYHRPFSPPNYDQSNGHSGT*	-1.664	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24150	PLRRIASKLLHSFICPCW			18	SLAY-screened peptide P2500			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTCCGGCGCATCGCTTCTAAGCTCCTGCACTCTTTTATCTGCCCCTGTTGGTAGCCCTAA	PLRRIASKLLHSFICPCW*P*	-1.664	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24151	PRYPYRVSTLLPSDSRLGRS			20	SLAY-screened peptide P2501			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGCTACCCTTATCGCGTGTCGACCCTGCTCCCGTCTGACTCGCGGCTTGGGAGGTCGTAA	PRYPYRVSTLLPSDSRLGRS*	-1.664	7.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24152	LPGLTTHCVYDGPTSDTSRA			20	SLAY-screened peptide P2502			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCGGGCCTCACTACTCATTGTGTCTATGACGGTCCCACGAGTGACACCTCTAGGGCTTAA	LPGLTTHCVYDGPTSDTSRA*	-1.664	0.000149	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24153	HCGMPDWSDYSFRYI			15	SLAY-screened peptide P2503			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGTGGCATGCCCGATTGGTCGGATTACTCTTTTCGCTATATCTAGTTCCAGACTCCGTAA	HCGMPDWSDYSFRYI*FQTP*	-1.664	0.024309	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24154	HRHMQAVPTPQVPARRTRGF			20	SLAY-screened peptide P2504			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCGCCACATGCAGGCTGTTCCCACGCCCCAGGTCCCTGCTCGTCGGACTCGCGGCTTCTAA	HRHMQAVPTPQVPARRTRGF*	-1.663	0.023872	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24155	PQA			3	SLAY-screened peptide P2505			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCAAGCATAAGACCAACTGTCGTAAGTACGGGTCGAAGAATATGCATAATCTCAACTAACT	PQA*DQLS*VRVEEYA*SQLT	-1.663	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24156	PDSGLVPFTHFGTAFYIHHA			20	SLAY-screened peptide P2506			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGATTCGGGCCTTGTCCCTTTCACCCATTTTGGCACGGCTTTTTATATCCACCACGCCTAA	PDSGLVPFTHFGTAFYIHHA*	-1.663	2.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24157	TNALY			5	SLAY-screened peptide P2507			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGAATGCCCTGTACTAGCGGCGCTTTCCCCTGTGGCTTTGGCCCGGTTGCGCCATCCACTAA	TNALY*RRFPLWLWPGCAIH*	-1.663	3.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24158	PTPIANRTDTPYLFTVYYYT			20	SLAY-screened peptide P2508			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACCCCTATTGCTAATAGGACGGATACCCCCTATTTGTTTACCGTTTATTATTACACGTAA	PTPIANRTDTPYLFTVYYYT*	-1.663	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24159	APAIFADYHPVCLFYARNTM			20	SLAY-screened peptide P2509			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCCGCTATCTTTGCTGATTATCACCCCGTTTGCCTGTTCTATGCTCGGAACACTATGTAA	APAIFADYHPVCLFYARNTM*	-1.662	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24160	STPAHPRASDFYYTASFYRN			20	SLAY-screened peptide P2510			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTACCCCCGCTCACCCTAGGGCGTCGGATTTTTACTACACTGCTTCGTTCTACCGTAACTAA	STPAHPRASDFYYTASFYRN*	-1.662	0.00536	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24161	NIDTWPSHFRLHTRFGRATP			20	SLAY-screened peptide P2511			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACATCGATACGTGGCCCTCCCATTTCCGCTTGCATACTCGCTTTGGCCGCGCTACTCCCTAA	NIDTWPSHFRLHTRFGRATP*	-1.662	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24162	AKLILNVGILLRRRTSSLSFN			21	SLAY-screened peptide P2512			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAAGCTCATTTTGAACGTGGGCATATTACTGAGACGCCGAACTTCCTCGTTAAGCTTTAAC	AKLILNVGILLRRRTSSLSFN	-1.662	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24163	CWHPKTKKGRYLVNHMPVVF			20	SLAY-screened peptide P2513			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTGGCACCCCAAGACTAAGAAGGGGCGCTACCTTGTCAACCACATGCCCGTTGTTTTCTAA	CWHPKTKKGRYLVNHMPVVF*	-1.661	0.000397	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24164	RPGSACPSSG			10	SLAY-screened peptide P2514			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCCGGCTCGGCCTGCCCGTCGAGCGGTTAGATCATCTTGCCTTTTTCCCAGTCTAGCTAA	RPGSACPSSG*IILPFSQSS*	-1.661	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24165	LFTAYSPRVITMVHTSRVIT			20	SLAY-screened peptide P2515			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTTCACCGCGTACAGCCCCAGGGTTATTACTATGGTCCACACGAGCCGCGTGATCACGTAA	LFTAYSPRVITMVHTSRVIT*	-1.661	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24166	CHRLSRNDASHCFPSARVPGN			21	SLAY-screened peptide P2516			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCACCGTCTGTCTCGCAATGACGCCTCGCACTGTTTTCCTTCAGCACGTGTCCCCGGTAAC	CHRLSRNDASHCFPSARVPGN	-1.66	0.001742	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24167	RCFTDDTDLLSPSMIVYYSI			20	SLAY-screened peptide P2517			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTGCTTCACTGACGATACTGATCTTCTCAGCCCTAGTATGATTGTCTATTACAGTATTTAA	RCFTDDTDLLSPSMIVYYSI*	-1.66	0.000653	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24168	GFLSTPPLRERRLFCIPRPS			20	SLAY-screened peptide P2518			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTTCCTTTCCACCCCGCCCCTTCGCGAGCGTAGGCTGTTTTGTATCCCGCGTCCCTCGTAA	GFLSTPPLRERRLFCIPRPS*	-1.659	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24169	SIDHSYLFWYI			11	SLAY-screened peptide P2519			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGATTGACCATAGTTACCTCTTCTGGTATATCTAGCCTACTGCTCATGTTAACATGAGGTAA	SIDHSYLFWYI*PTAHVNMR*	-1.659	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24170	IVAYYVYTYFPGRSLNNIPP			20	SLAY-screened peptide P2520			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGTGGCTTACTATGTTTACACCTACTTTCCGGGGCGTTCGCTGAATAACATCCCGCCTTAA	IVAYYVYTYFPGRSLNNIPP*	-1.658	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24171	SRPPSNTIAITFHNNRTFFF			20	SLAY-screened peptide P2521			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCAGGCCCCCTTCCAACACGATCGCGATTACGTTCCATAACAACCGTACTTTCTTTTTTTAA	SRPPSNTIAITFHNNRTFFF*	-1.658	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24172	DFLPCHLFHRPHNSNGEYVG			20	SLAY-screened peptide P2522			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTTCTTGCCCTGCCATCTTTTCCACCGTCCCCATAATTCTAACGGTGAGTATGTTGGCTAA	DFLPCHLFHRPHNSNGEYVG*	-1.658	0.001482	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24173	LSQSASPCYC			10	SLAY-screened peptide P2523			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCCCAGAGTGCTTCTCCTTGCTATTGTTAGAGGTACGCGCTCGATGATGGTGACTCCTAA	LSQSASPCYC*RYALDDGDS*	-1.658	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24174	RMSQLRALL			9	SLAY-screened peptide P2524			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATGAGCCAGCTCCGTGCCCTTCTCTAGTCTTGTTCCACTAAGGCGGAGCGTTGCGTCTAA	RMSQLRALL*SCSTKAERCV*	-1.658	0.000249	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24175	YARMNITLVGMCQVFYFIIR			20	SLAY-screened peptide P2525			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGCGAGGATGAACATTACCCTCGTTGGTATGTGTCAGGTCTTTTATTTCATTATCCGCTAA	YARMNITLVGMCQVFYFIIR*	-1.657	0.001025	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24176	IPYQHLFDVINRFLHIRKMN			20	SLAY-screened peptide P2526			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCCCTATCAGCATTTGTTTGACGTCATTAACCGTTTCCTTCACATTCGCAAGATGAACTAA	IPYQHLFDVINRFLHIRKMN*	-1.657	0.0005	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24177	PTDKTSARSNDSCLHVANRV			20	SLAY-screened peptide P2527			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACCGATAAGACCAGCGCTCGTAGCAACGATAGCTGTTTGCATGTTGCCAACAGGGTGTAA	PTDKTSARSNDSCLHVANRV*	-1.657	0.006651	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24178	TLFDWYMGCYPLAQRDIIYL			20	SLAY-screened peptide P2528			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTGTTCGACTGGTACATGGGTTGTTATCCCCTGGCTCAGCGCGATATTATCTACTTGTAA	TLFDWYMGCYPLAQRDIIYL*	-1.656	0.015514	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24179	QSYSNNFHLIHRTDLPFTCT			20	SLAY-screened peptide P2529			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGAGTTACTCCAACAACTTTCATCTTATCCATCGGACCGATCTTCCTTTTACTTGCACGTAA	QSYSNNFHLIHRTDLPFTCT*	-1.656	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24180	PLMYCHPYGYPYRAFIFDTL			20	SLAY-screened peptide P2530			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTGATGTACTGCCACCCGTATGGGTATCCGTATCGCGCCTTCATCTTCGACACTTTGTAA	PLMYCHPYGYPYRAFIFDTL*	-1.656	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24181	HWIGYALTLEALHCLFYSHP			20	SLAY-screened peptide P2531			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGGATCGGGTACGCCCTTACGCTTGAGGCTCTTCACTGCCTCTTTTATTCTCATCCTTAA	HWIGYALTLEALHCLFYSHP*	-1.656	0.044457	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24182	PLPCWLV			7	SLAY-screened peptide P2532			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTGCCCTGCTGGCTTGTCTAGGCGGCCTCGCTGTCCAGGATGTGTGCTTTCCGCAGGTAA	PLPCWLV*AASLSRMCAFRR*	-1.655	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24183	GCSPTPLGIFFPSLVVRQIR			20	SLAY-screened peptide P2533			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGTGCAGCCCTACGCCTCTTGGTATTTTCTTCCCCAGTCTCGTTGTCCGCCAGATCCGCTAA	GCSPTPLGIFFPSLVVRQIR*	-1.655	0.012436	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24184	RFFLFFIRRRTTFKAWSINS			20	SLAY-screened peptide P2534			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTTCTTCTTGTTCTTTATTCGCCGGAGGACGACCTTTAAGGCGTGGAGCATCAACAGCTAA	RFFLFFIRRRTTFKAWSINS*	-1.655	0.000421	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24185	RLAGHATFHVTLLVYLSTRN			20	SLAY-screened peptide P2535			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCTTGCTGGCCATGCCACCTTCCACGTGACTCTCCTGGTCTATCTCTCCACTAGGAACTAA	RLAGHATFHVTLLVYLSTRN*	-1.655	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24186	SGACWDKTDTYQLG			14	SLAY-screened peptide P2536			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGGCGCCTGCTGGGATAAGACGGACACCTATCAGCTTGGTTAGTTCTCTATCGGGTCTTAA	SGACWDKTDTYQLG*FSIGS*	-1.654	0.000967	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24187	SAIANASVVSSIACAIGHIT			20	SLAY-screened peptide P2537			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGCTATCGCTAATGCTTCCGTGGTGTCGTCCATTGCCTGCGCTATTGGGCACATTACGTAA	SAIANASVVSSIACAIGHIT*	-1.654	0.031658	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24188	FLRPLVRSLLRRLLVMGWRRN			21	SLAY-screened peptide P2538			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTTGCGTCCGTTGGTTCGCTCCCTCCTCCGGAGGTTATTAGTTATGGGATGGCGCCGTAAC	FLRPLVRSLLRRLLVMGWRRN	-1.654	0.008316	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24189	PSVSNFPHRCD			11	SLAY-screened peptide P2539			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCTGTGTCTAACTTCCCGCACCGCTGCGATTAGCGTGCCTTGCGTTGTAGGACGGACTAA	PSVSNFPHRCD*RALRCRTD*	-1.654	0.000943	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24190	PRNDGSGYITPKIRTLFENQ			20	SLAY-screened peptide P2540			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGGAATGATGGCTCCGGTTATATCACTCCGAAGATTCGGACCCTTTTTGAGAACCAGTAA	PRNDGSGYITPKIRTLFENQ*	-1.654	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24191	PSRHVDRPGNNLGNVNSATD			20	SLAY-screened peptide P2541			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAGCAGGCATGTGGACAGGCCGGGCAACAATTTGGGCAATGTTAATAGCGCTACGGACTAA	PSRHVDRPGNNLGNVNSATD*	-1.653	0.026102	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24192	FLVESDTAAAYNPETCDPNV			20	SLAY-screened peptide P2542			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCTGGTGGAGTCTGATACCGCCGCCGCCTACAACCCCGAGACTTGCGACCCGAATGTTTAA	FLVESDTAAAYNPETCDPNV*	-1.653	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24193	PRTMSYNSACTCSELNCLDP			20	SLAY-screened peptide P2543			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGGACCATGAGTTATAACTCCGCCTGCACGTGTTCCGAGCTGAACTGTCTGGATCCCTAA	PRTMSYNSACTCSELNCLDP*	-1.653	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24194	NPHGPIRTNNDRHVTREPTY			20	SLAY-screened peptide P2544			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCCCCACGGCCCTATTCGCACGAACAACGATCGTCATGTCACCCGGGAGCCTACCTACTAA	NPHGPIRTNNDRHVTREPTY*	-1.653	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24195	SPQGL			5	SLAY-screened peptide P2545			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCCCCAGGGCCTTTAGCACCACCATTAGCGTATGGACAACCAGCTCGCCTTTAGTCAGTAA	SPQGL*HHH*RMDNQLAFSQ*	-1.652	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24196	RLSFNPPYPGLRAHLPGTFL			20	SLAY-screened peptide P2546			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCTGTCGTTTAACCCGCCCTATCCGGGCCTCCGCGCGCACCTCCCGGGCACTTTCCTCTAA	RLSFNPPYPGLRAHLPGTFL*	-1.652	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24197	TFNSVPAHGQVRNFYHHFIR			20	SLAY-screened peptide P2547			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTTTAACAGTGTGCCGGCTCATGGGCAGGTCCGTAACTTCTACCATCATTTTATCCGTTAA	TFNSVPAHGQVRNFYHHFIR*	-1.651	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24198	HCSPPVTWLWGSSIPHAYLL			20	SLAY-screened peptide P2548			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTGTTCCCCTCCCGTGACGTGGCTCTGGGGTAGCTCTATCCCTCACGCTTACCTCTTGTAA	HCSPPVTWLWGSSIPHAYLL*	-1.651	0.000104	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24199	CPIMFLQRSPVTMSLNPDSL			20	SLAY-screened peptide P2549			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCCATCATGTTCCTCCAGCGTAGCCCCGTTACCATGAGTCTTAACCCTGATTCCTTGTAA	CPIMFLQRSPVTMSLNPDSL*	-1.65	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24200	TTSLDTGMLHPRNTQTSNSD			20	SLAY-screened peptide P2550			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTACTTCCCTTGACACTGGCATGCTGCATCCTCGTAATACTCAGACGTCGAATTCTGATTAA	TTSLDTGMLHPRNTQTSNSD*	-1.65	0.0311	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24201	PPSTDLTQCHQPLPWRYLRM			20	SLAY-screened peptide P2551			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCTAGTACGGACCTGACCCAGTGCCATCAGCCCCTCCCCTGGCGTTACCTGCGCATGTAA	PPSTDLTQCHQPLPWRYLRM*	-1.65	0.000707	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24202	LGGSRDMPFIHPSAADTCGH			20	SLAY-screened peptide P2552			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGGCGGTTCTCGCGACATGCCGTTTATTCATCCCTCGGCGGCGGATACTTGCGGTCATTAA	LGGSRDMPFIHPSAADTCGH*	-1.65	0.025147	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24203	GRSTGHLPWTIWKLFLCVAH			20	SLAY-screened peptide P2553			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCGTTCTACTGGGCATCTTCCTTGGACTATCTGGAAGCTGTTTCTGTGTGTGGCCCACTAA	GRSTGHLPWTIWKLFLCVAH*	-1.65	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24204	THPPAH			6	SLAY-screened peptide P2554			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCACCCCCCCGCCCACTAGCCGCTTTCCAGTCAGGTTTTTTACCACACGGACTACTAGTAA	THPPAH*PLSSQVFYHTDY**	-1.65	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24205	LLPSPCRSV			9	SLAY-screened peptide P2555			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTGCCCTCGCCTTGCAGGAGCGTCTAGTAGTTGCCCTTGGCCTTCAAGAATGTGCTCTAA	LLPSPCRSV**LPLAFKNVL*	-1.649	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24206	YYNRFGGRCSSL			12	SLAY-screened peptide P2556			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTACAACCGTTTCGGAGGTCGATGTTCGTCGTTATAAGATGTTGAATAATTCGAATAACTA	YYNRFGGRCSSL*DVE*FE*L	-1.648	0.003453	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24207	PRASNYALALDDFTPLPLTA			20	SLAY-screened peptide P2557			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAGGGCTAGTAATTACGCGCTTGCTCTGGATGACTTTACGCCTCTTCCGTTGACCGCTTAA	PRASNYALALDDFTPLPLTA*	-1.648	0.000359	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24208	RCHRYAANGQSHGLNESIHP			20	SLAY-screened peptide P2558			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTGCCACCGCTACGCGGCTAATGGGCAGTCTCATGGGTTGAATGAGTCTATCCACCCGTAA	RCHRYAANGQSHGLNESIHP*	-1.648	0.000146	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24209	HTRVNSHYSSCPSLGPRLRSN			21	SLAY-screened peptide P2559			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACGCGTGTCAATAGTCACTACAGTTCCTGCCCAAGTCTTGGGCCCCGTCTTCGATCTAAC	HTRVNSHYSSCPSLGPRLRSN	-1.648	0.001817	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24210	HTIDALPYDLFRSSPTCFQY			20	SLAY-screened peptide P2560			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACCATTGATGCGCTGCCGTACGACCTGTTTCGCTCGTCGCCCACGTGCTTTCAGTACTAA	HTIDALPYDLFRSSPTCFQY*	-1.648	0.006516	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24211	RPYHCISILSMILVPRPMVT			20	SLAY-screened peptide P2561			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCCTACCATTGTATTTCTATTCTCTCCATGATCTTGGTTCCGCGGCCTATGGTGACTTAA	RPYHCISILSMILVPRPMVT*	-1.648	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24212	RYPFDTTHILPNPSADSYKC			20	SLAY-screened peptide P2562			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTATCCGTTCGATACCACCCACATCTTGCCCAACCCGTCTGCTGATTCTTATAAGTGCTAA	RYPFDTTHILPNPSADSYKC*	-1.647	0.002015	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24213	CHFHPHHYRVITSVPL			16	SLAY-screened peptide P2563			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCACTTCCATCCGCATCACTACCGGGTGATCACGTCGGTGCCTCTCTAGTTCGGTGATTAA	CHFHPHHYRVITSVPL*FGD*	-1.647	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24214	NLYHCPAHPLYITAAGNAIS			20	SLAY-screened peptide P2564			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTTGTACCATTGCCCGGCTCATCCTCTGTATATTACGGCTGCGGGTAATGCTATTTCCTAA	NLYHCPAHPLYITAAGNAIS*	-1.647	0.02038	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24215	TNVRYACYRLFGGIRICYCP			20	SLAY-screened peptide P2565			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAACGTGCGCTATGCCTGTTATCGGTTGTTCGGGGGTATCCGCATCTGTTATTGTCCCTAA	TNVRYACYRLFGGIRICYCP*	-1.647	0.008385	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24216	HPDHGLSALFHTGMYAASL			19	SLAY-screened peptide P2566			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCGGACCACGGTCTCTCTGCCCTTTTCCACACCGGTATGTATGCTGCTTCCTTGTAGTAA	HPDHGLSALFHTGMYAASL**	-1.647	0.026016	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24217	NETWPSPHGCRTVVAPMRIA			20	SLAY-screened peptide P2567			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGAGACGTGGCCCTCTCCCCATGGTTGCCGGACCGTGGTCGCCCCGATGCGGATCGCTTAA	NETWPSPHGCRTVVAPMRIA*	-1.647	0.001213	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24218	PTVRNANSFCLRKIILSRDW			20	SLAY-screened peptide P2568			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACTGTTCGTAATGCCAATAGTTTTTGTTTGCGCAAGATCATTCTCAGTCGTGATTGGTAA	PTVRNANSFCLRKIILSRDW*	-1.646	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24219	RSMRRRYVHLTRRALHNNAC			20	SLAY-screened peptide P2569			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTCTATGCGCCGCCGCTACGTCCATCTTACTCGTCGGGCGCTTCATAACAATGCTTGCTAA	RSMRRRYVHLTRRALHNNAC*	-1.646	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24220	VSAGITRLLHLCDYFTTMCF			20	SLAY-screened peptide P2570			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGAGCGCTGGGATCACCAGGCTGCTCCATCTTTGTGACTACTTCACGACTATGTGCTTCTAA	VSAGITRLLHLCDYFTTMCF*	-1.645	0.01066	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24221	RAAEYLFALSLDPLLFYSYQ			20	SLAY-screened peptide P2571			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGGCTGCCGAGTATCTCTTTGCCCTGTCCCTCGATCCTCTTCTCTTCTACAGTTATCAGTAA	RAAEYLFALSLDPLLFYSYQ*	-1.645	0.011149	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24222	LPVYCWYTLITPICSCDEYV			20	SLAY-screened peptide P2572			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCCGTCTACTGTTGGTACACCCTCATTACGCCTATCTGTAGCTGCGATGAGTACGTTTAA	LPVYCWYTLITPICSCDEYV*	-1.644	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24223	PHRACPSGSSPFPLLPFFFLN			21	SLAY-screened peptide P2573			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCATCGGGCCTGCCCCTCGGGATCGTCGCCGTTCCCACTGCTACCCTTTTTCTTTCTTAAC	PHRACPSGSSPFPLLPFFFLN	-1.644	0.005076	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24224	HCTSYQHNTPSMISNYYDIM			20	SLAY-screened peptide P2574			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGCACCAGTTATCAGCACAATACGCCCTCCATGATCTCGAATTACTATGATATCATGTAA	HCTSYQHNTPSMISNYYDIM*	-1.644	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24225	NYDALRLFDLRNYQFTNNAS			20	SLAY-screened peptide P2575			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTATGATGCTCTCCGCCTGTTCGATCTTCGCAACTATCAGTTCACTAATAATGCCTCGTAA	NYDALRLFDLRNYQFTNNAS*	-1.643	0.005656	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24226	SNSRENHPTVHLCSVSNVLY			20	SLAY-screened peptide P2576			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTAACTCTAGGGAGAATCACCCGACGGTGCATTTGTGCTCTGTTAGCAACGTGCTGTATTAA	SNSRENHPTVHLCSVSNVLY*	-1.643	0.004283	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24227	INLMVMYTTASSLSHYWDLD			20	SLAY-screened peptide P2577			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAATCTCATGGTCATGTATACTACGGCGTCGAGCCTGTCTCACTACTGGGACCTGGATTAA	INLMVMYTTASSLSHYWDLD*	-1.643	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24228	LQVVLPYGCYSSNYFCYNLL			20	SLAY-screened peptide P2578			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCAGGTTGTTCTGCCTTATGGTTGTTACTCGTCCAACTATTTTTGTTACAATCTCCTTTAA	LQVVLPYGCYSSNYFCYNLL*	-1.643	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24229	CECANICYYPPYWNII			16	SLAY-screened peptide P2579			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGAGTGCGCTAATATTTGTTATTACCCTCCCTATTGGAACATCATTTAGTATGTCTAGTAA	CECANICYYPPYWNII*YV**	-1.643	0.015522	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24230	RATSPVIS			8	SLAY-screened peptide P2580			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGCTACTTCTCCTGTTATCTCTTAGTGTCGTGACCACTCTGTGGCGGTGTGCATGAACTAA	RATSPVIS*CRDHSVAVCMN*	-1.643	0.000374	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24231	TRIILDGYPSNPLNALANDN			20	SLAY-screened peptide P2581			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCGCATCATCTTGGATGGCTATCCGTCTAATCCTCTGAATGCCTTGGCTAACGACAATTAA	TRIILDGYPSNPLNALANDN*	-1.643	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24232	LSGCYAPSTHVLHAWEKISV			20	SLAY-screened peptide P2582			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAGCGGGTGTTATGCTCCTAGTACTCACGTGCTCCATGCCTGGGAGAAGATCAGCGTCTAA	LSGCYAPSTHVLHAWEKISV*	-1.642	0.000666	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24233	ALHFSLLLFRFCRSRTCRHC			20	SLAY-screened peptide P2583			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTTGCATTTCAGTCTTCTCCTCTTTAGGTTCTGTCGCTCGCGTACGTGTAGGCATTGTTAA	ALHFSLLLFRFCRSRTCRHC*	-1.642	0.000152	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24234	PQAMGRRITSRSGTHTWKMC			20	SLAY-screened peptide P2584			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCAGGCCATGGGCCGGCGTATTACCTCCCGTTCGGGTACCCATACCTGGAAGATGTGTTAA	PQAMGRRITSRSGTHTWKMC*	-1.642	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24235	PLSCTLEVVSNYCTGHFKCP			20	SLAY-screened peptide P2585			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCAGCTGCACCCTCGAGGTGGTGTCTAACTACTGTACTGGGCACTTCAAGTGTCCTTAA	PLSCTLEVVSNYCTGHFKCP*	-1.642	0.000156	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24236	HAPSSTLTLGNLPQSDHTVA			20	SLAY-screened peptide P2586			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCGCCTTCGTCTACGCTTACCTTGGGTAATTTGCCCCAGTCCGACCACACGGTCGCTTAA	HAPSSTLTLGNLPQSDHTVA*	-1.642	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24237	RLAGTLGHTLTVFYARSIAR			20	SLAY-screened peptide P2587			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTTGGCTGGCACCCTGGGCCACACCCTGACGGTTTTCTATGCGCGGTCTATCGCCCGGTAA	RLAGTLGHTLTVFYARSIAR*	-1.641	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24238	PAWSYSLFWLYFTVYMCFEK			20	SLAY-screened peptide P2588			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGCGTGGTCGTACTCCCTGTTTTGGCTTTATTTCACCGTTTATATGTGCTTTGAGAAGTAA	PAWSYSLFWLYFTVYMCFEK*	-1.641	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24239	HHHHHFNIPLNTVPYSYCLA			20	SLAY-screened peptide P2589			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCATCATCACCACTTCAACATCCCTCTTAATACCGTGCCTTATTCGTATTGCCTCGCTTAA	HHHHHFNIPLNTVPYSYCLA*	-1.641	0.01151	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24240	CDLYFYL			7	SLAY-screened peptide P2590			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGATCTTTATTTTTATTTGTAGCTTGCGGGGCATAATATTCGTTTCAATCATATTCTGTAA	CDLYFYL*LAGHNIRFNHIL*	-1.641	0.000741	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24241	STHGDAIYVHSCLWCVIHTR			20	SLAY-screened peptide P2591			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCACGCATGGCGACGCGATTTACGTTCATTCTTGCCTGTGGTGTGTCATTCATACGCGTTAA	STHGDAIYVHSCLWCVIHTR*	-1.641	5.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24242	CLRSSHL			7	SLAY-screened peptide P2592			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCTCAGGTCTTCTCACCTCTAGCGTGACTGTGCTGCGCATTGTACTGTCGTTATCCCTTAA	CLRSSHL*RDCAAHCTVVIP*	-1.641	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24243	KYRMTPRFPTICHRIHTMYN			20	SLAY-screened peptide P2593			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGTACAGGATGACGCCCCGTTTTCCCACTATCTGTCATCGGATTCATACGATGTATAATTAA	KYRMTPRFPTICHRIHTMYN*	-1.639	0.000181	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24244	DPTAAMYTPCTATVVT			16	SLAY-screened peptide P2594			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCCTACCGCCGCCATGTACACCCCGTGCACCGCCACCGTCGTCACGTAGAATCGCGCCTAA	DPTAAMYTPCTATVVT*NRA*	-1.639	0.017461	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24245	HPVHTTFSNPCDITHTPYTFK			21	SLAY-screened peptide P2595			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCCGTCCATACCACCTTTTCCAACCCCTGCGACATCACTCACACGCCGTACACTTTCAAG	HPVHTTFSNPCDITHTPYTFK	-1.639	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24246	TGRRSNVSKHSANIYVNSKG			20	SLAY-screened peptide P2596			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGGCCGCAGGTCCAATGTTAGTAAGCATTCGGCTAACATTTATGTTAACTCTAAGGGCTAA	TGRRSNVSKHSANIYVNSKG*	-1.638	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24247	PLSSNGDCHLFIHYDYV			17	SLAY-screened peptide P2597			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTGTCTTCTAATGGTGACTGCCATCTGTTTATTCACTACGACTATGTTTAGCGCCATTAA	PLSSNGDCHLFIHYDYV*RH*	-1.638	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24248	RQRFGAIYTATSKYDCTCIR			20	SLAY-screened peptide P2598			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCAGAGGTTTGGCGCTATCTATACGGCTACGTCTAAGTATGACTGCACCTGTATCAGGTAA	RQRFGAIYTATSKYDCTCIR*	-1.638	0.03453	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24249	QQLKHYLNLMSLYLHAFLRH			20	SLAY-screened peptide P2599			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCAGCTTAAGCACTACCTTAACCTTATGTCCCTGTACCTGCATGCGTTTCTCCGCCACTAA	QQLKHYLNLMSLYLHAFLRH*	-1.638	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24250	LHHSYTPVFPFNNSLPCVLF			20	SLAY-screened peptide P2600			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCATCATTCTTATACTCCGGTGTTCCCGTTCAATAACAGTCTTCCCTGCGTCTTGTTCTAA	LHHSYTPVFPFNNSLPCVLF*	-1.637	0.000723	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24251	CETPVLQKPFLSDAHTRGPR			20	SLAY-screened peptide P2601			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGAGACTCCTGTCCTTCAGAAGCCTTTTCTCTCCGATGCGCATACGCGCGGTCCTCGGTAA	CETPVLQKPFLSDAHTRGPR*	-1.637	0.00272	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24252	LADKLRGLLCKYCYRTQICT			20	SLAY-screened peptide P2602			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGCTGACAAGCTTCGGGGGCTGCTCTGCAAGTATTGCTACCGCACTCAGATTTGCACGTAA	LADKLRGLLCKYCYRTQICT*	-1.636	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24253	YRNRARRTFDLHLPPLLVTT			20	SLAY-screened peptide P2603			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAGGAACCGCGCTCGTCGTACCTTTGACCTTCACCTGCCTCCTTTGCTGGTCACTACTTAA	YRNRARRTFDLHLPPLLVTT*	-1.636	0.002006	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24254	RDPT			4	SLAY-screened peptide P2604			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGACCCGACTTAGACTGCTTTGACCCATAATCGTATTGTCCTGGGCTCTGGGATGTGCTAA	RDPT*TALTHNRIVLGSGMC*	-1.636	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24255	NDAGIIERPNVMHRITRDPF			20	SLAY-screened peptide P2605			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGATGCTGGGATTATTGAGCGCCCCAATGTCATGCACCGCATTACCCGTGACCCCTTCTAA	NDAGIIERPNVMHRITRDPF*	-1.636	1.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24256	RPFPKHDFNAILRL			14	SLAY-screened peptide P2606			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCTTTTCCTAAGCACGACTTCAACGCCATTTTGCGTCTTTAGAAGCTTGCCGAGGATTAA	RPFPKHDFNAILRL*KLAED*	-1.636	7.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24257	NTIRSHLSLLYLIPIEHHEA			20	SLAY-screened peptide P2607			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATACCATCCGCAGTCACCTTTCCCTTCTTTACCTTATTCCTATTGAGCATCATGAGGCCTAA	NTIRSHLSLLYLIPIEHHEA*	-1.635	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24258	SYLSQYKSNYIRLLGISTSP			20	SLAY-screened peptide P2608			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTATCTCTCCCAGTATAAGTCCAATTATATTAGGCTGCTGGGCATCTCGACGAGTCCCTAA	SYLSQYKSNYIRLLGISTSP*	-1.635	0.000385	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24259	PSADFFNLKNKCYNYIELFV			20	SLAY-screened peptide P2609			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCGGCCGACTTTTTCAATCTTAAGAATAAGTGCTATAACTATATTGAGTTGTTTGTTTAA	PSADFFNLKNKCYNYIELFV*	-1.634	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24260	TQYECFTAIHNRASFDHLLV			20	SLAY-screened peptide P2610			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCAGTATGAGTGTTTTACTGCGATCCACAATCGCGCCTCCTTTGACCATCTTTTGGTTTAA	TQYECFTAIHNRASFDHLLV*	-1.634	0.002696	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24261	YRFLPLEVVLLHGW			14	SLAY-screened peptide P2611			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCGCTTTTTACCGCTGGAAGTCGTTTTACTTCATGGTTGGTAACACGAACAGGTAGTAACT	YRFLPLEVVLLHGW*HEQVVT	-1.634	0.021318	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24262	FATGWAGFRDCLTMNSTHIN			20	SLAY-screened peptide P2612			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGCTACCGGGTGGGCTGGTTTCCGTGATTGCTTGACCATGAATAGTACGCACATTAACTAA	FATGWAGFRDCLTMNSTHIN*	-1.634	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24263	VPHTPNVNYSGVQSVTEINV			20	SLAY-screened peptide P2613			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCCTCATACTCCTAACGTTAACTACTCGGGGGTGCAGTCCGTTACCGAGATTAACGTGTAA	VPHTPNVNYSGVQSVTEINV*	-1.634	0.017019	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24264	YSLMLSCYSTRYIFIGENSP			20	SLAY-screened peptide P2614			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTCGTTGATGCTGTCCTGCTACTCCACTCGTTATATTTTCATCGGGGAGAACAGCCCCTAA	YSLMLSCYSTRYIFIGENSP*	-1.634	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24265	CSHTWFVDITIFHCSDFSG			19	SLAY-screened peptide P2615			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTCGCACACCTGGTTCGTGGATATTACTATTTTCCACTGCAGTGATTTCAGTGGTTAGTAA	CSHTWFVDITIFHCSDFSG**	-1.634	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24266	ISGTLPHGNTWRKCAHIPDY			20	SLAY-screened peptide P2616			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAGCGGTACCCTTCCCCATGGCAACACTTGGAGGAAGTGCGCCCACATTCCGGATTACTAA	ISGTLPHGNTWRKCAHIPDY*	-1.633	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24267	SPRVHSLEEGKYQGAMIDSD			20	SLAY-screened peptide P2617			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCCCCGGGTTCACTCCTTGGAGGAGGGTAAGTACCAGGGTGCCATGATTGACTCCGACTAA	SPRVHSLEEGKYQGAMIDSD*	-1.633	0.000293	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24268	PSASASNSFIPTKNLTCRGP			20	SLAY-screened peptide P2618			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGTGCGTCCGCGAGCAATAGTTTCATTCCCACGAAGAATCTTACCTGTAGGGGTCCGTAA	PSASASNSFIPTKNLTCRGP*	-1.633	2.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24269	SA			2	SLAY-screened peptide P2619			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGCCTAGTGACCGACGCCCTTCCTTTTTGTCGTACCTACTTGAACACTACGTATGTCTAAC	SA**PTPFLFVVPT*TLRMSN	-1.633	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24270	LFGTLVLFSYYLDTHAR			17	SLAY-screened peptide P2620			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTTCGGTACCCTTGTCCTCTTTAGCTATTACCTTGATACGCATGCTCGGTAGCGCTACTAA	LFGTLVLFSYYLDTHAR*RY*	-1.633	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24271	RHSNSRSNNHSLGSSSNFVV			20	SLAY-screened peptide P2621			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCACTCTAATTCGCGCTCGAATAATCATTCCCTGGGGTCCTCGAGTAATTTTGTGGTTTAA	RHSNSRSNNHSLGSSSNFVV*	-1.633	0.000128	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24272	LLPKA			5	SLAY-screened peptide P2622			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTGCCGAAGGCCTAGATCTCCGAGTCTCTTAGGGCGTATGACGTCCTCTCTGACAGTTAA	LLPKA*ISESLRAYDVLSDS*	-1.633	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24273	YLNNITGPMVAKHTDTFYPA			20	SLAY-screened peptide P2623			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCTTAATAATATTACGGGGCCTATGGTTGCCAAGCACACTGATACTTTTTATCCCGCTTAA	YLNNITGPMVAKHTDTFYPA*	-1.632	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24274	HFTIY			5	SLAY-screened peptide P2624			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTTTACGATCTACTAGTCGGTCTATCTTACCCAGCGGCACCATGTTACGAGCTAGTCGTAA	HFTIY*SVYLTQRHHVTS*S*	-1.632	0.009444	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24275	CAPLCFAIHRYKASFQNLKD			20	SLAY-screened peptide P2625			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCCCCCCTGTGCTTTGCCATTCATCGTTATAAGGCTTCGTTTCAGAATCTCAAGGACTAA	CAPLCFAIHRYKASFQNLKD*	-1.632	0.018012	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24276	RARPACAHAHTRKTASASIS			20	SLAY-screened peptide P2626			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCCCGTCCCGCGTGTGCTCACGCCCATACGCGCAAGACCGCGTCGGCGTCGATTTCCTAA	RARPACAHAHTRKTASASIS*	-1.631	0.015024	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24277	DTNMIASIWRNRDNLYKSED			20	SLAY-screened peptide P2627			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACACCAACATGATCGCCAGTATCTGGCGTAACCGTGACAACCTTTATAAGTCTGAGGATTAA	DTNMIASIWRNRDNLYKSED*	-1.631	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24278	PSRARVIVSVNINLYVYLFF			20	SLAY-screened peptide P2628			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCTCGGGCTCGCGTTATTGTTAGCGTCAATATCAATCTCTACGTTTACCTCTTTTTTTAA	PSRARVIVSVNINLYVYLFF*	-1.631	0.000653	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24279	PYDSKDPCSARNLTFLYHQL			20	SLAY-screened peptide P2629			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTATGACTCGAAGGACCCCTGCAGTGCCCGTAATCTGACTTTTCTTTATCACCAGCTCTAA	PYDSKDPCSARNLTFLYHQL*	-1.63	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24280	RIPLNSNVTRSDSSICTKCI			20	SLAY-screened peptide P2630			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATTCCCCTCAATTCTAACGTCACCCGTTCCGACTCTAGTATTTGCACTAAGTGTATTTAA	RIPLNSNVTRSDSSICTKCI*	-1.629	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24281	TGPETNRPSRN			11	SLAY-screened peptide P2631			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGGTCCCGAGACCAACCGCCCCTCCAGGAACTAGACCTGGTCTATGCTGTCGATGATCTAA	TGPETNRPSRN*TWSMLSMI*	-1.629	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24282	SLHRHFHIYTSSLPYYFFPT			20	SLAY-screened peptide P2632			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCTCCATAGGCATTTTCACATTTACACGAGCTCTTTGCCCTATTATTTTTTCCCCACTTAA	SLHRHFHIYTSSLPYYFFPT*	-1.629	0.002693	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24283	HSLSCRQYIVEPLYILNNSI			20	SLAY-screened peptide P2633			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTCCTTGAGTTGTCGTCAGTACATCGTGGAGCCGTTGTATATCCTGAATAATAGTATTTAA	HSLSCRQYIVEPLYILNNSI*	-1.629	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24284	RVEKPTLRSSVYFRTLLNQN			20	SLAY-screened peptide P2634			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGTGGAGAAGCCTACTCTGCGCAGCAGTGTCTACTTTAGGACGCTGTTGAACCAGAACTAA	RVEKPTLRSSVYFRTLLNQN*	-1.629	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24285	QPRTSRYFCPHITCPYGFYY			20	SLAY-screened peptide P2635			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCCCGGACTAGTAGGTATTTTTGCCCCCATATTACTTGTCCCTATGGCTTTTACTACTAA	QPRTSRYFCPHITCPYGFYY*	-1.628	0.000141	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24286	ALQFLSSLADYCNNPPSGSA			20	SLAY-screened peptide P2636			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCTTCAGTTCTTGAGTTCCCTGGCCGACTATTGCAATAATCCCCCGAGTGGCTCCGCCTAA	ALQFLSSLADYCNNPPSGSA*	-1.628	0.031369	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24287	RTVLRTFHFPSSLQFSLCKS			20	SLAY-screened peptide P2637			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACCGTGCTTCGGACGTTTCATTTTCCTTCGTCTCTTCAGTTTTCGTTGTGTAAGTCGTAA	RTVLRTFHFPSSLQFSLCKS*	-1.628	0.000788	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24288	FG			2	SLAY-screened peptide P2638			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGGGTAGGTGCCCTCTCCGTATTAGAGTACCCGTGTCATTCCTTATTCTCCCTTCCGTTAA	FG*VPSPY*STRVIPYSPFR*	-1.628	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24289	LNR			3	SLAY-screened peptide P2639			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAACCGTTGAAGAGCCTCCTGAATGTCCATTGTCATGTCACTAACTACCACCCGAACTAAC	LNR*RAS*MSIVMSLTTTRTN	-1.628	0.0441	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24290	RPCCSTARYRDLFA			14	SLAY-screened peptide P2640			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCCTGTTGCTCCACTGCTCGCTACCGGGACCTGTTTGCTTAGTGCTCCATCCACTTCTAA	RPCCSTARYRDLFA*CSIHF*	-1.627	0.026413	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24291	HPYTPLCTDFWGSSMILVSS			20	SLAY-screened peptide P2641			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCTTACACCCCTCTGTGTACGGACTTTTGGGGTTCGTCGATGATTCTCGTGTCCTCGTAA	HPYTPLCTDFWGSSMILVSS*	-1.627	0.013358	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24292	NTAWRHSTNFPSSSCPFVPL			20	SLAY-screened peptide P2642			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACACCGCCTGGCGCCACAGTACGAACTTTCCGTCCTCTTCTTGTCCGTTCGTTCCTCTTTAA	NTAWRHSTNFPSSSCPFVPL*	-1.627	0.045004	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24293	EPVISPSTTDKCCPNLFWYL			20	SLAY-screened peptide P2643			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCCGGTCATCAGCCCTTCGACGACGGACAAGTGTTGCCCTAACCTCTTCTGGTACCTTTAA	EPVISPSTTDKCCPNLFWYL*	-1.627	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24294	YSPRSHS			7	SLAY-screened peptide P2644			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACAGCCCGCGCTCTCACTCCTAGACTTCCGCTTGTTATGCTCCTGCTAATCTTTGCTGCTAA	YSPRSHS*TSACYAPANLCC*	-1.627	0.000335	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24295	DPGWNYHFYSHRLQFHTNHS			20	SLAY-screened peptide P2645			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCCCGGTTGGAATTATCATTTCTATTCGCATCGCCTGCAGTTCCACACCAACCACTCTTAA	DPGWNYHFYSHRLQFHTNHS*	-1.627	0.027259	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24296	TLKCPLCIRRHE			12	SLAY-screened peptide P2646			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTTAAGTGCCCCCTCTGTATTCGGCGCCATGAGTAGAGCACCGATAACATCACTCCTTAA	TLKCPLCIRRHE*STDNITP*	-1.627	0.00369	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24297	VTIDDSILRRTVPKRGNFNV			20	SLAY-screened peptide P2647			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTACCATCGACGATTCGATTTTGCGGCGTACGGTCCCGAAGCGGGGTAACTTTAACGTTTAA	VTIDDSILRRTVPKRGNFNV*	-1.626	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24298	QGDLSSRALAIYFMPISRNG			20	SLAY-screened peptide P2648			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGGGGATCTCTCCTCGCGCGCCCTCGCTATCTACTTTATGCCGATTTCGAGGAATGGCTAA	QGDLSSRALAIYFMPISRNG*	-1.626	0.000439	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24299	RAYFTWPDGCRLLIT			15	SLAY-screened peptide P2649			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGCCTATTTCACGTGGCCCGATGGCTGCCGTCTCCTCATCACGTAGACGTCGAGCAAGTAA	RAYFTWPDGCRLLIT*TSSK*	-1.626	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24300	AAPTWHFSFYYQSSSTFSND			20	SLAY-screened peptide P2650			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCTCCGACTTGGCACTTTTCTTTTTACTATCAGAGCTCGAGCACGTTTAGCAACGATTAA	AAPTWHFSFYYQSSSTFSND*	-1.625	0.001122	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24301	GDPSH			5	SLAY-screened peptide P2651			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTGACCCCTCCCACTAGTCCTGTCAGATTAGCACTACTATTAACGCTACTAGGAAGCCGTAA	GDPSH*SCQISTTINATRKP*	-1.625	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24302	PMTPRMNRKHYYTHLHADSS			20	SLAY-screened peptide P2652			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATGACGCCTCGCATGAACAGGAAGCATTATTATACCCACCTGCATGCTGATTCGAGCTAA	PMTPRMNRKHYYTHLHADSS*	-1.624	0.005715	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24303	MYVPGSWSSPPYFFL			15	SLAY-screened peptide P2653			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTACGTTCCCGGGTCTTGGTCATCACCCCCTTACTTCTTTCTTTGAGTCCCTCCAGCACTC	MYVPGSWSSPPYFFL*VPPAL	-1.624	0.002943	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24304	TNNDNGTNNLDGLVSVQCCP			20	SLAY-screened peptide P2654			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAATAACGACAATGGTACTAATAACCTTGACGGCCTCGTTAGCGTGCAGTGTTGCCCTTAA	TNNDNGTNNLDGLVSVQCCP*	-1.624	0.001004	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24305	PKTTLAYSLVHRSRKCHLWS			20	SLAY-screened peptide P2655			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAAGACGACCTTGGCTTACTCCCTGGTTCATCGTAGTAGGAAGTGCCACTTGTGGAGTTAA	PKTTLAYSLVHRSRKCHLWS*	-1.624	0.002672	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24306	SSSPCNNSTRVARILPLYSL			20	SLAY-screened peptide P2656			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGAGTTCTCCGTGCAACAACAGTACCCGGGTTGCTCGTATTCTTCCGCTTTATTCCCTCTAA	SSSPCNNSTRVARILPLYSL*	-1.624	4.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24307	APSLSLLQRTVFSLSTCTSV			20	SLAY-screened peptide P2657			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCCTTCTCTGAGTCTTCTCCAGAGGACCGTGTTTTCTCTGAGCACCTGCACCAGTGTTTAA	APSLSLLQRTVFSLSTCTSV*	-1.624	0.032204	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24308	PLRDITRRIWRGLLMKVADF			20	SLAY-screened peptide P2658			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTCCGCGATATTACGCGTCGCATTTGGCGGGGTCTCTTGATGAAGGTCGCTGACTTCTAA	PLRDITRRIWRGLLMKVADF*	-1.624	0.001333	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24309	RPSRSVRVQCPNFSKPNSYG			20	SLAY-screened peptide P2659			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCGTCGCGTTCTGTCAGGGTTCAGTGTCCGAATTTCTCTAAGCCTAATAGCTACGGTTAA	RPSRSVRVQCPNFSKPNSYG*	-1.624	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24310	MLLLIHLGPARTARPEYPKK			20	SLAY-screened peptide P2660			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCTGTTGCTTATCCATCTTGGCCCCGCTCGCACGGCGCGCCCCGAGTATCCGAAGAAGTAA	MLLLIHLGPARTARPEYPKK*	-1.624	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24311	CLHPIRGKMLPVPTWLAFTV			20	SLAY-screened peptide P2661			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTTCACCCTATTCGGGGCAAGATGCTGCCTGTCCCTACTTGGCTTGCTTTCACCGTCTAA	CLHPIRGKMLPVPTWLAFTV*	-1.623	0.002627	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24312	ENTSIRAHFTVHCSYLSTDY			20	SLAY-screened peptide P2662			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGAATACTAGTATTCGGGCTCATTTTACCGTCCATTGTTCGTACCTGTCTACTGACTACTAA	ENTSIRAHFTVHCSYLSTDY*	-1.623	0.000637	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24313	DAYGFPAPYAPLGLCTFHQL			20	SLAY-screened peptide P2663			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGCGTACGGCTTTCCCGCGCCGTACGCCCCTCTGGGCCTCTGCACCTTCCATCAGCTGTAA	DAYGFPAPYAPLGLCTFHQL*	-1.623	0.003307	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24314	CARNVPHHRYPGPGPPRLPL			20	SLAY-screened peptide P2664			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCCCGCAATGTGCCTCACCACCGGTATCCTGGCCCTGGGCCTCCTCGCCTGCCCCTCTAA	CARNVPHHRYPGPGPPRLPL*	-1.623	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24315	HVTVVYIAHSHLQSGNPPMD			20	SLAY-screened peptide P2665			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGTTACGGTTGTTTACATTGCTCACAGCCATTTGCAGTCTGGTAACCCTCCTATGGACTAA	HVTVVYIAHSHLQSGNPPMD*	-1.622	0.007508	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24316	RPVFAHSRYIGAMSKIALLK			20	SLAY-screened peptide P2666			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCGGTCTTCGCGCACTCCCGTTATATCGGTGCCATGAGCAAGATTGCCCTTTTGAAGTAA	RPVFAHSRYIGAMSKIALLK*	-1.622	0.001513	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24317	SRALPASSNTTDPTTAMHSW			20	SLAY-screened peptide P2667			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCGTGCCCTCCCGGCGAGTTCTAACACTACCGATCCCACCACCGCGATGCACTCGTGGTAA	SRALPASSNTTDPTTAMHSW*	-1.622	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24318	PHLISQT			7	SLAY-screened peptide P2668			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCATCTTATTAGTCAGACGTAGCAGCAGTAGCCGAAGATGAGTAGTATTTTGCTTGACTAA	PHLISQT*QQ*PKMSSILLD*	-1.621	3.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24319	DFHCAPKINRFPEGYGTLQT			20	SLAY-screened peptide P2669			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTTCCATTGTGCGCCGAAGATCAATCGGTTTCCGGAGGGCTATGGCACCCTGCAGACTTAA	DFHCAPKINRFPEGYGTLQT*	-1.621	0.002189	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24320	VR			2	SLAY-screened peptide P2670			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCGTTAGATTACTCCCTCCGCTAGGTTTATTCGCACTATCGCGCGTCTGTTCTACTCCTAA	VR*ITPSARFIRTIARLFYS*	-1.621	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24321	PLERFCSCRHLDFYHSLAID			20	SLAY-screened peptide P2671			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTGGAGCGTTTCTGTAGCTGCCGCCATTTGGATTTCTATCATTCGCTGGCGATCGACTAA	PLERFCSCRHLDFYHSLAID*	-1.621	7.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24322	FWVPHHGRCCIHHHYLILPR			20	SLAY-screened peptide P2672			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTGGGTTCCCCATCATGGTCGCTGTTGCATCCACCACCACTATCTTATCCTGCCGCGCTAA	FWVPHHGRCCIHHHYLILPR*	-1.621	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24323	CFRGHPLNVLGNLLIASHTR			20	SLAY-screened peptide P2673			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTTCCGCGGGCACCCTCTTAACGTGCTTGGTAATTTGCTTATTGCTAGCCACACGCGCTAA	CFRGHPLNVLGNLLIASHTR*	-1.62	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24324	PPCPFVKITSNTCSCDSANI			20	SLAY-screened peptide P2674			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTTGTCCCTTCGTGAAGATTACTTCGAACACTTGTAGTTGTGATAGCGCGAATATCTAA	PPCPFVKITSNTCSCDSANI*	-1.62	0.031151	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24325	PLPRICSGPPNN			12	SLAY-screened peptide P2675			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTGCCTCGCATTTGTTCTGGGCCCCCTAATAACTAGTCTACCACTCTTCTGTAGTACTAA	PLPRICSGPPNN*STTLL*Y*	-1.62	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24326	NTLAIVDIEPFVRGCDPAHA			20	SLAY-screened peptide P2676			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATACGCTCGCCATTGTCGATATCGAGCCCTTTGTGCGGGGGTGCGATCCGGCTCATGCTTAA	NTLAIVDIEPFVRGCDPAHA*	-1.62	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24327	LTHNSTSSNPNSITI			15	SLAY-screened peptide P2677			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGACGCATAATAGTACTTCCAGTAACCCTAACTCCATTACCATTTAGTGTTGTTTTATTTAA	LTHNSTSSNPNSITI*CCFI*	-1.619	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24328	CPRYPVMDVL			10	SLAY-screened peptide P2678			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCGCGGTACCCCGTGATGGACGTGCTGTAGATCGGGCGTATGATGAGCCAGAACCACTAA	CPRYPVMDVL*IGRMMSQNH*	-1.619	0.000771	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24329	PYLSSGISALCPIGFLYSFV			20	SLAY-screened peptide P2679			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTATCTTTCTTCTGGGATCTCCGCCCTGTGTCCCATTGGGTTCCTGTACAGCTTTGTCTAA	PYLSSGISALCPIGFLYSFV*	-1.618	3.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24330	PPNIRCKSWTLNPRNFRLRP			20	SLAY-screened peptide P2680			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCTAATATCAGGTGCAAGAGTTGGACTCTCAACCCTAGGAACTTTCGTCTTAGGCCCTAA	PPNIRCKSWTLNPRNFRLRP*	-1.618	0.002721	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24331	HPYFPLAMYIL			11	SLAY-screened peptide P2681			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCGTACTTTCCGCTCGCCATGTACATTCTCTAGAATCTCTCTGGGATTAAGGCCACCTAA	HPYFPLAMYIL*NLSGIKAT*	-1.618	0.007115	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24332	QPGTSPSLANPTCYHSLKIY			20	SLAY-screened peptide P2682			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCGGGGACCTCTCCGAGTTTGGCTAATCCGACCTGCTATCATTCGCTGAAGATTTATTAA	QPGTSPSLANPTCYHSLKIY*	-1.618	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24333	PKFTSHDPCAYSESNLFFKN			20	SLAY-screened peptide P2683			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAAGTTTACCTCTCATGACCCCTGCGCCTACAGCGAGTCTAATCTTTTTTTTAAGAACTAA	PKFTSHDPCAYSESNLFFKN*	-1.618	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24334	RYIGTLNTYPDYHILPSYIL			20	SLAY-screened peptide P2684			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTATATCGGCACCCTTAATACCTACCCTGACTACCATATTCTGCCCTCGTACATTTTGTAA	RYIGTLNTYPDYHILPSYIL*	-1.618	0.010262	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24335	NVIPPYCFHYDYHHCCNRYH			20	SLAY-screened peptide P2685			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGTCATTCCTCCCTACTGTTTCCACTACGACTATCATCATTGTTGCAACCGCTACCATTAA	NVIPPYCFHYDYHHCCNRYH*	-1.618	0.010177	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24336	LWQFMCPCQLLQFPIWYAPM			20	SLAY-screened peptide P2686			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTGGCAGTTTATGTGCCCTTGCCAGCTTCTCCAGTTCCCTATCTGGTACGCCCCTATGTAA	LWQFMCPCQLLQFPIWYAPM*	-1.618	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24337	NPSCPVNCLSWSPLTFYFLI			20	SLAY-screened peptide P2687			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCCCTCCTGCCCTGTGAACTGTCTTTCCTGGTCGCCTCTGACGTTCTACTTCTTGATTTAA	NPSCPVNCLSWSPLTFYFLI*	-1.617	0.000144	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24338	CPTFNYFHSAAPWMLPVASTS			21	SLAY-screened peptide P2688			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCCACTTTTAACTACTTTCATTCCGCCGCCCCGTGGATGCTCCCCGTCGCGAGCACGAGC	CPTFNYFHSAAPWMLPVASTS	-1.617	0.000237	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24339	LLSQSLRGPGNEPIHLLSVC			20	SLAY-screened peptide P2689			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTTGTCGCAGAGCCTGCGCGGTCCTGGCAACGAGCCTATTCACTTGCTTAGCGTGTGCTAA	LLSQSLRGPGNEPIHLLSVC*	-1.617	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24340	AASHVYSMDPSWFHTYPLLS			20	SLAY-screened peptide P2690			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGCTTCCCATGTTTACTCTATGGATCCGTCCTGGTTCCACACTTATCCTTTGCTCTCCTAA	AASHVYSMDPSWFHTYPLLS*	-1.617	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24341	RSVG			4	SLAY-screened peptide P2691			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTCGGTTGGCTAGGCGCCCGGTTCCTATAAGAACTCGATGTTCATATCTATGCGAGTTAAC	RSVG*APGSYKNSMFISMRVN	-1.616	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24342	LLDKLLHGFCNLRGHNR			17	SLAY-screened peptide P2692			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCTTGATAAGTTGTTGCACGGGTTCTGTAATCTCCGCGGGCATAATCGCTAGTAGAATTAA	LLDKLLHGFCNLRGHNR**N*	-1.616	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24343	SYFRSNVYNTVPTSRRCPLP			20	SLAY-screened peptide P2693			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTACTTCCGCTCTAACGTCTATAACACGGTCCCGACGAGTAGGCGCTGTCCCCTCCCCTAA	SYFRSNVYNTVPTSRRCPLP*	-1.616	0.004191	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24344	GSFTAHNHVNICSNRPLLNF			20	SLAY-screened peptide P2694			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTCTTTTACCGCCCACAATCATGTTAATATTTGCAGTAATAGGCCGCTGCTCAATTTTTAA	GSFTAHNHVNICSNRPLLNF*	-1.616	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24345	LNTDGSLLRPV			11	SLAY-screened peptide P2695			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAATACGGACGGCTCTCTGCTTAGGCCTGTTTAGGGTATCATCCACCCTGTTTTGTTCTAA	LNTDGSLLRPV*GIIHPVLF*	-1.615	1.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24346	TN			2	SLAY-screened peptide P2696			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAATTAGTTTAGTTACTGTCATAATCATCCTATCTACGTCAGTAATTTCCAGAACCACTAA	TN*FSYCHNHPIYVSNFQNH*	-1.615	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24347	RSRARDPPTQRRGPGDTLYS			20	SLAY-screened peptide P2697			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCGCGGGCGCGTGACCCGCCCACGCAGCGTAGGGGCCCCGGCGATACTCTGTATTCGTAA	RSRARDPPTQRRGPGDTLYS*	-1.615	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24348	PLSSQRYSDSHLGPNHPRPT			20	SLAY-screened peptide P2698			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTAGCAGTCAGCGGTATTCCGACTCTCACTTGGGCCCCAATCATCCTCGCCCTACTTAA	PLSSQRYSDSHLGPNHPRPT*	-1.615	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24349	RNCVTRYTIRPNIKYMGPFK			20	SLAY-screened peptide P2699			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAATTGCGTCACCCGGTATACTATCCGTCCCAACATCAAGTATATGGGCCCCTTCAAGTAA	RNCVTRYTIRPNIKYMGPFK*	-1.615	2.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24350	TGYRFYTYNTPLHH			14	SLAY-screened peptide P2700			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGGTTACCGCTTTTACACTTACAACACTCCGCTTCATCACTAGTACCTGACGTACGGCTAA	TGYRFYTYNTPLHH*YLTYG*	-1.614	9.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24351	IDAAHYVRFDTYTYDDISSD			20	SLAY-screened peptide P2701			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGATGCGGCGCACTATGTCCGCTTTGACACGTATACTTACGACGATATTTCTTCGGACTAA	IDAAHYVRFDTYTYDDISSD*	-1.614	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24352	CLTYYAQ			7	SLAY-screened peptide P2702			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTCACGTACTACGCCCAGTAGATCCATCTTAATAGCCGCGCTGCGACCGCCGCCAACTAA	CLTYYAQ*IHLNSRAATAAN*	-1.614	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24353	SRPTSRFLEGHSRSMTEPAS			20	SLAY-screened peptide P2703			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCGTCCTACCTCCCGCTTTCTGGAGGGCCATTCGCGGTCCATGACGGAGCCCGCGTCTTAA	SRPTSRFLEGHSRSMTEPAS*	-1.614	0.00306	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24354	KKAPICDRTPLTSPFYTWMV			20	SLAY-screened peptide P2704			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGAAGGCGCCCATTTGTGACCGCACCCCGCTGACGAGCCCGTTCTACACTTGGATGGTTTAA	KKAPICDRTPLTSPFYTWMV*	-1.613	0.000592	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24355	PPLFTRVTTSVPE			13	SLAY-screened peptide P2705			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTCTCTTTACTCGTGTCACCACCTCGGTTCCTGAGTAGCATTCTTATGCTCACCCGTAA	PPLFTRVTTSVPE*HSYAHP*	-1.613	0.028453	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24356	HMPPNNASVDLHNSSSIIEM			20	SLAY-screened peptide P2706			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATATGCCCCCGAATAACGCGTCGGTTGATCTTCATAATTCCAGCTCTATCATCGAGATGTAA	HMPPNNASVDLHNSSSIIEM*	-1.613	0.02047	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24357	SNCRIRTVNIH			11	SLAY-screened peptide P2707			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAACTGTCGGATTCGTACCGTCAATATCCATTAGGCCTTCAGTCGCAGTAAGTCGGTTTAA	SNCRIRTVNIH*AFSRSKSV*	-1.613	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24358	RHNRDVIPYSPAPCLPMYNN			20	SLAY-screened peptide P2708			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCACAACCGTGATGTCATCCCCTACTCTCCGGCTCCGTGCCTTCCCATGTATAACAATTAA	RHNRDVIPYSPAPCLPMYNN*	-1.613	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24359	AVPPPHTLDDHHFNNSKTFS			20	SLAY-screened peptide P2709			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGTCCCTCCGCCTCATACTCTTGATGATCATCACTTTAACAACAGTAAGACCTTTTCTTAA	AVPPPHTLDDHHFNNSKTFS*	-1.613	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24360	VQSLDVQYMAICH			13	SLAY-screened peptide P2710			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCAGTCTCTTGATGTGCAGTACATGGCTATTTGTCATTAGTAGGGGACCGCTTAGGTCTAA	VQSLDVQYMAICH**GTA*V*	-1.613	0.001558	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24361	SAHIISSIE			9	SLAY-screened peptide P2711			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGCCCATATTATTTCTTCCATTGAGTAGGACCAGCAGTCCATCGAGAGCTTTAAGAGGTAA	SAHIISSIE*DQQSIESFKR*	-1.613	0.016074	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24362	SRQPQYYYGYIFMSPGRISN			20	SLAY-screened peptide P2712			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCGGCAGCCCCAGTACTATTACGGGTATATTTTCATGTCTCCCGGGCGGATTTCCAACTAA	SRQPQYYYGYIFMSPGRISN*	-1.612	0.000422	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24363	QGRILNKGIIPLLPCLAMTGN			21	SLAY-screened peptide P2713			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGGTCGCATCTTGAACAAGGGAATTATCCCTCTTTTACCCTGCCTAGCAATGACTGGTAAC	QGRILNKGIIPLLPCLAMTGN	-1.612	0.000363	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24364	FWVMRHRSNEGLGSDQTGSI			20	SLAY-screened peptide P2714			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTGGGTCATGAGGCATAGGTCGAACGAGGGTCTGGGCTCCGATCAGACCGGGAGCATTTAA	FWVMRHRSNEGLGSDQTGSI*	-1.612	0.021618	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24365	TPYYCAPLSTFKVTELDITI			20	SLAY-screened peptide P2715			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCCCTATTACTGCGCCCCCCTGTCGACCTTTAAGGTTACCGAGCTGGACATCACGATTTAA	TPYYCAPLSTFKVTELDITI*	-1.612	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24366	VDRFPWNAKLYCGSAIPHNI			20	SLAY-screened peptide P2716			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGACCGTTTTCCCTGGAATGCGAAGTTGTATTGTGGCTCGGCCATTCCGCATAATATCTAA	VDRFPWNAKLYCGSAIPHNI*	-1.612	0.000461	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24367	SFFHASLTEHYCTHQLSLAQ			20	SLAY-screened peptide P2717			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTTCTTCCACGCCAGCCTTACTGAGCATTACTGTACTCACCAGCTGTCCCTCGCCCAGTAA	SFFHASLTEHYCTHQLSLAQ*	-1.612	0.001974	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24368	SMYFNARLGRAVFSSHPLDN			20	SLAY-screened peptide P2718			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTATGTACTTTAACGCCCGCCTGGGTCGGGCCGTGTTCAGCAGCCATCCGTTGGATAATTAA	SMYFNARLGRAVFSSHPLDN*	-1.612	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24369	FIFSIVYARRNLPKRLPSTS			20	SLAY-screened peptide P2719			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTATTTTCTCTATTGTCTATGCCCGGCGGAATCTTCCGAAGCGTTTGCCCTCCACTAGTTAA	FIFSIVYARRNLPKRLPSTS*	-1.612	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24370	PCYPNIMLLLLLSLIRSYWL			20	SLAY-screened peptide P2720			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGCTATCCCAATATCATGTTGCTTCTGCTCCTGAGTCTGATTCGCTCCTATTGGCTTTAA	PCYPNIMLLLLLSLIRSYWL*	-1.612	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24371	PSRYTAASAPPPSIRSTHAY			20	SLAY-screened peptide P2721			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGTAGGTACACGGCCGCTTCGGCTCCTCCGCCCAGCATCCGTTCGACCCACGCCTATTAA	PSRYTAASAPPPSIRSTHAY*	-1.611	0.006705	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24372	MTSSFTLPSVICNQLPSPPI			20	SLAY-screened peptide P2722			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGACCAGCAGTTTCACTCTCCCTTCGGTTATTTGTAATCAGCTTCCTTCTCCGCCGATCTAA	MTSSFTLPSVICNQLPSPPI*	-1.611	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24373	HRPDKINNNETYTELSTTRN			20	SLAY-screened peptide P2723			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCGCCCTGACAAGATCAATAACAATGAGACTTATACTGAGTTGTCCACCACTAGGAATTAA	HRPDKINNNETYTELSTTRN*	-1.611	2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24374	NCSYHLTVR			9	SLAY-screened peptide P2724			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTGCAGTTACCACCTCACGGTTCGCTAGGACACCAATGGCCTTTACGTTACGTGCGACTAA	NCSYHLTVR*DTNGLYVTCD*	-1.611	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24375	LPFAHACPTDLPAMHLTT			18	SLAY-screened peptide P2725			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCGTTTGCTCATGCCTGCCCGACTGATCTTCCCGCTATGCATTTGACCACCTAGGGCTAA	LPFAHACPTDLPAMHLTT*G*	-1.611	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24376	PLYIFARYPDIADTCKIPPM			20	SLAY-screened peptide P2726			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTCTACATCTTTGCGCGCTACCCGGATATCGCCGATACCTGCAAGATCCCGCCGATGTAA	PLYIFARYPDIADTCKIPPM*	-1.611	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24377	SDLRNTLFWYMTLCGPYALF			20	SLAY-screened peptide P2727			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGACTTGCGCAACACCCTGTTTTGGTACATGACGCTGTGCGGTCCCTATGCGCTTTTCTAA	SDLRNTLFWYMTLCGPYALF*	-1.611	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24378	TSRNAVANAVGDLATETSII			20	SLAY-screened peptide P2728			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTCCCGTAACGCTGTGGCGAATGCGGTGGGTGATCTGGCCACCGAGACTTCCATTATCTAA	TSRNAVANAVGDLATETSII*	-1.61	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24379	RFYQQTPPTVGHLPYCTSIV			20	SLAY-screened peptide P2729			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTTCTACCAGCAGACGCCGCCTACGGTGGGCCACTTGCCTTATTGCACCAGTATCGTTTAA	RFYQQTPPTVGHLPYCTSIV*	-1.61	5.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24380	RPECYVFHPVKLAVARLPRT			20	SLAY-screened peptide P2730			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCGGAGTGCTATGTCTTCCACCCGGTGAAGCTGGCTGTCGCCCGCCTTCCCCGGACCTAA	RPECYVFHPVKLAVARLPRT*	-1.61	0.002824	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24381	PATTHYPHSVRCLDIHNFPH			20	SLAY-screened peptide P2731			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCGACGACCCATTACCCCCACAGTGTGCGTTGCTTGGATATCCATAATTTCCCGCATTAA	PATTHYPHSVRCLDIHNFPH*	-1.609	0.043794	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24382	KPGSSILGHSSIPSFLIPTT			20	SLAY-screened peptide P2732			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCCGGGTAGTTCTATTCTTGGTCATTCGAGTATCCCCTCTTTTTTGATTCCGACGACGTAA	KPGSSILGHSSIPSFLIPTT*	-1.609	0.000277	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24383	PSISSGPYGSPHNLARFYFR			20	SLAY-screened peptide P2733			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTCTATCTCTTCGGGCCCTTATGGTAGTCCTCATAATCTTGCCCGCTTTTATTTCCGGTAA	PSISSGPYGSPHNLARFYFR*	-1.609	0.001291	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24384	CALYTYRFFHNHFNACFMYT			20	SLAY-screened peptide P2734			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCCCTGTACACTTATCGCTTCTTTCATAACCATTTCAATGCCTGTTTCATGTATACCTAA	CALYTYRFFHNHFNACFMYT*	-1.609	0.001275	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24385	PIKQSCFARPIAVTNTSHNL			20	SLAY-screened peptide P2735			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATCAAGCAGTCCTGTTTTGCCCGCCCCATCGCCGTTACGAATACGAGTCACAATCTGTAA	PIKQSCFARPIAVTNTSHNL*	-1.608	0.000315	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24386	HWDPLPSNPPASTPDARHAY			20	SLAY-screened peptide P2736			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTGGGATCCTCTCCCCAGTAATCCGCCTGCTAGTACTCCCGACGCGCGTCACGCGTATTAA	HWDPLPSNPPASTPDARHAY*	-1.608	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24387	TARNRYHATTITHYGARKNC			20	SLAY-screened peptide P2737			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGCCAGGAACCGTTACCATGCGACTACTATTACTCACTATGGGGCCCGTAAGAATTGCTAA	TARNRYHATTITHYGARKNC*	-1.608	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24388	NWD			3	SLAY-screened peptide P2738			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTGGGACTAGAAGAGTGCTCCCCACTGCTACAGCACTTCGGATCGCCATACTATCACCTAA	NWD*KSAPHCYSTSDRHTIT*	-1.608	0.000794	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24389	LTRPPNDRY			9	SLAY-screened peptide P2739			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACCCGGCCTCCCAACGACCGCTACTAGGAGACTATGGATCCCAAGCGCAACCAGGACTAA	LTRPPNDRY*ETMDPKRNQD*	-1.608	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24390	DPTHDKDDGLLCAPSTIRFL			20	SLAY-screened peptide P2740			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCCGACCCATGACAAGGATGACGGCCTCTTGTGCGCCCCTTCTACCATCCGTTTCCTCTAA	DPTHDKDDGLLCAPSTIRFL*	-1.608	0.010076	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24391	RLIVTVSN			8	SLAY-screened peptide P2741			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTGATCGTTACGGTGAGCAATTAGTTCAACTTTGTGAAGGGGAGTAAGGCTGCCGCTTAA	RLIVTVSN*FNFVKGSKAAA*	-1.607	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24392	YVCVARNY			8	SLAY-screened peptide P2742			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGTTTGTGTTGCCCGTAATTACTAGTCTACTCCGCTTCTCCGCCCCATTATGACGGCGTAA	YVCVARNY*STPLLRPIMTA*	-1.607	0.000808	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24393	RPGRDSMFDD			10	SLAY-screened peptide P2743			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCCGGCCGCGATTCCATGTTTGACGACTAGATTGACGTGTCTATCGCTCACGTTGGTAAC	RPGRDSMFDD*IDVSIAHVGN	-1.607	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24394	VLSS			4	SLAY-screened peptide P2744			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCTTAGCAGTTAGCTCACTGTTCGATTGGTTGTACGACCCGTGCGAACAGCGATAGTTAAC	VLSS*LTVRLVVRPVRTAIVN	-1.607	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24395	DC			2	SLAY-screened peptide P2745			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTGCTAGAGCCACCTTTGCAATTCGAATTCCGTTTCTAGGGAGCATAGCAATTGTGAGTAA	DC*SHLCNSNSVSREHSNCE*	-1.607	0.000417	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24396	GVQTPCQLINEYLCCVTIHI			20	SLAY-screened peptide P2746			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTGTGCAGACGCCCTGTCAGCTCATCAACGAGTACCTGTGTTGTGTGACCATTCACATTTAA	GVQTPCQLINEYLCCVTIHI*	-1.606	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24397	SNFPRAADHTLIT			13	SLAY-screened peptide P2747			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCAACTTTCCTAGGGCGGCTGACCACACTCTCATTACGTAGACTAGCTATTTGAAGCGCTAA	SNFPRAADHTLIT*TSYLKR*	-1.606	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24398	LAYVGFFICFLFTRRDADCT			20	SLAY-screened peptide P2748			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGCTTATGTCGGTTTTTTCATTTGTTTCCTGTTTACCCGCCGGGACGCCGACTGTACCTAA	LAYVGFFICFLFTRRDADCT*	-1.606	3.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24399	TVAT			4	SLAY-screened peptide P2749			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGTTGCTACTTAGTCGGTGGCGCTTTGCATGCAGATGACTCATGCCGCCGATAACACGTAA	TVAT*SVALCMQMTHAADNT*	-1.606	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24400	HTSPCPCKYYSLICFSIISN			20	SLAY-screened peptide P2750			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACGTCTCCTTGTCCTTGTAAGTATTATAGTCTTATCTGTTTTTCCATTATCTCCAACTAA	HTSPCPCKYYSLICFSIISN*	-1.606	3.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24401	PGLPCVLVYYNMIFTLPCAF			20	SLAY-screened peptide P2751			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGGCTCCCTTGTGTCCTTGTGTATTACAATATGATTTTCACTTTGCCCTGCGCCTTTTAA	PGLPCVLVYYNMIFTLPCAF*	-1.605	0.002646	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24402	CHVFDIYMRRYDTLCFVYYS			20	SLAY-screened peptide P2752			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCACGTCTTTGACATTTACATGCGCCGTTATGACACGCTCTGCTTCGTTTACTATAGTTAA	CHVFDIYMRRYDTLCFVYYS*	-1.605	2.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24403	AVSRMFNNDTLDCHF			15	SLAY-screened peptide P2753			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGTCAGCCGTATGTTCAATAACGATACCCTTGACTGTCATTTCTAGACGGATACTGATTAA	AVSRMFNNDTLDCHF*TDTD*	-1.605	0.0039	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24404	CRARSYWGPFYLLDNVTSQY			20	SLAY-screened peptide P2754			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGCGCCCGTAGCTATTGGGGCCCGTTTTATTTGCTTGACAACGTTACGTCCCAGTATTAA	CRARSYWGPFYLLDNVTSQY*	-1.605	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24405	AGTRAAGTSDDISYSPIECL			20	SLAY-screened peptide P2755			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGGTACCCGTGCGGCCGGTACCTCGGATGATATTAGTTACAGCCCTATTGAGTGCCTCTAA	AGTRAAGTSDDISYSPIECL*	-1.604	0.018948	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24406	PIKASPMTNYWATSNPLDDI			20	SLAY-screened peptide P2756			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATTAAGGCGTCCCCTATGACGAATTACTGGGCCACCAGCAATCCCCTTGATGACATCTAA	PIKASPMTNYWATSNPLDDI*	-1.604	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24407	HSPTLYPRGDTNCRPPAASV			20	SLAY-screened peptide P2757			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAGCCCGACCCTGTATCCGCGCGGTGACACTAATTGTCGGCCGCCCGCTGCCAGCGTGTAA	HSPTLYPRGDTNCRPPAASV*	-1.604	0.001656	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24408	CESTSGFERFFYISSNNPVI			20	SLAY-screened peptide P2758			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGAGTCCACCTCTGGCTTTGAGCGGTTCTTTTACATTTCTTCCAATAACCCCGTTATCTAA	CESTSGFERFFYISSNNPVI*	-1.604	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24409	RSIFSVERLISNKPHHLQIF			20	SLAY-screened peptide P2759			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTCTATCTTTTCGGTGGAGCGGCTGATCTCCAATAAGCCTCACCACCTTCAGATTTTCTAA	RSIFSVERLISNKPHHLQIF*	-1.603	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24410	HCFHLMASNPTTSVAPSRRS			20	SLAY-screened peptide P2760			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGTTTTCATCTTATGGCCTCCAACCCTACCACCAGCGTTGCGCCTTCTAGGCGGTCCTAA	HCFHLMASNPTTSVAPSRRS*	-1.602	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24411	CSAPRDKPYEPHPANSPYTN			20	SLAY-screened peptide P2761			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAGCGCGCCGCGCGACAAGCCTTACGAGCCGCATCCGGCCAACTCCCCTTATACGAACTAA	CSAPRDKPYEPHPANSPYTN*	-1.601	0.035271	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24412	IVINCNFVNI			10	SLAY-screened peptide P2762			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGTTATTAATTGTAACTTCGTTAATATCTAGATGGCCACTTGGCAGTAGTTCAGTACTTAA	IVINCNFVNI*MATWQ*FST*	-1.601	0.000559	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24413	AEGINKYLGTK			11	SLAY-screened peptide P2763			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGAGGGGATCAACAAGTATCTCGGTACCAAGTAGTACACTCGTGTTTGTGCCGAGCACTAA	AEGINKYLGTK*YTRVCAEH*	-1.601	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24414	PPANMSS			7	SLAY-screened peptide P2764			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCGCCAATATGTCTAGCTAGGTTTCTGATTTTCTGTCCGGTCGGGCGCTGCGGAGGTAA	PPANMSS*VSDFLSGRALRR*	-1.6	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24415	PHNSSFRPRIRESSGFGNLS			20	SLAY-screened peptide P2765			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCATAACTCCTCCTTCCGGCCGCGTATCCGCGAGTCCAGCGGCTTTGGCAATCTGTCTTAA	PHNSSFRPRIRESSGFGNLS*	-1.6	0.00276	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24416	VRMCAATWYMPS			12	SLAY-screened peptide P2766			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCGTATGTGCGCTGCGACTTGGTACATGCCCAGTTAGATTCACGTGATGGTCATCCGGTAA	VRMCAATWYMPS*IHVMVIR*	-1.6	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24417	LMPTLSLIRCGTVLNRQPPS			20	SLAY-screened peptide P2767			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGATGCCCACCCTTAGTCTCATTCGCTGCGGGACTGTGCTCAATCGGCAGCCCCCTAGTTAA	LMPTLSLIRCGTVLNRQPPS*	-1.6	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24418	DRADFSCDHYSYHFLHYTSI			20	SLAY-screened peptide P2768			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCGTGCCGACTTTTCCTGCGACCATTACAGTTACCATTTTCTGCACTACACTTCCATCTAA	DRADFSCDHYSYHFLHYTSI*	-1.6	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24419	EAVGVYMNPYSLRPCELYLI			20	SLAY-screened peptide P2769			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGCTGTCGGCGTCTACATGAATCCGTATAGTTTGCGTCCTTGTGAGCTTTATCTCATCTAA	EAVGVYMNPYSLRPCELYLI*	-1.6	0.026238	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24420	NVHNDMGN			8	SLAY-screened peptide P2770			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGTCCACAACGACATGGGTAACTAGCTGCTTTTGTACACTAAGAACACTTAGGATCTGTAA	NVHNDMGN*LLLYTKNT*DL*	-1.6	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24421	SRHMCANAVDIMPLGSNVHR			20	SLAY-screened peptide P2771			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCGGCATATGTGCGCTAACGCTGTTGATATCATGCCTCTCGGGAGTAATGTGCATCGGTAA	SRHMCANAVDIMPLGSNVHR*	-1.599	0.001738	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24422	SNTSSHGIPMRCMSIMRLASN			21	SLAY-screened peptide P2772			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAACACGAGTTCTCATGGGATTCCTATGCGCTGTATGTCTATTATGAGATTAGCATCTAAC	SNTSSHGIPMRCMSIMRLASN	-1.599	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24423	EVLPVEPPTRHIGNCKTSQY			20	SLAY-screened peptide P2773			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGTGTTGCCTGTGGAGCCTCCTACTCGGCACATTGGGAATTGTAAGACTAGCCAGTACTAA	EVLPVEPPTRHIGNCKTSQY*	-1.599	0.010247	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24424	CYIGLTWIRISPLCYLSNTH			20	SLAY-screened peptide P2774			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTATATTGGCCTGACCTGGATTAGGATTAGTCCTCTCTGCTATCTGTCGAACACTCATTAA	CYIGLTWIRISPLCYLSNTH*	-1.599	0.018239	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24425	MHFISLRRTPSCPVPNNFPH			20	SLAY-screened peptide P2775			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCACTTCATCTCCTTGCGCCGCACTCCGTCGTGCCCTGTGCCTAACAATTTTCCCCATTAA	MHFISLRRTPSCPVPNNFPH*	-1.599	0.034069	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24426	PTSTNEKLTWNRNIPPHRRN			20	SLAY-screened peptide P2776			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACGAGCACCAATGAGAAGCTGACGTGGAACCGTAACATTCCTCCTCACCGTAGGAACTAA	PTSTNEKLTWNRNIPPHRRN*	-1.599	0.033001	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24427	ATNAYNVSHSGNRSLFGDEV			20	SLAY-screened peptide P2777			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTACGAATGCTTACAATGTCAGTCATAGTGGCAACCGCTCCTTGTTCGGGGATGAGGTTTAA	ATNAYNVSHSGNRSLFGDEV*	-1.598	0.003019	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24428	CCHTDILIPYAHSTWYHDHH			20	SLAY-screened peptide P2778			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTGCCACACCGACATTCTCATTCCCTATGCTCATTCTACGTGGTACCATGACCATCACTAA	CCHTDILIPYAHSTWYHDHH*	-1.598	0.033525	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24429	IRNRSHCYLSLSDHSDLPCT			20	SLAY-screened peptide P2779			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCGTAACCGTTCCCACTGCTACCTTTCTCTTTCTGACCACAGCGACTTGCCGTGTACCTAA	IRNRSHCYLSLSDHSDLPCT*	-1.598	0.002458	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24430	FGA			3	SLAY-screened peptide P2780			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGGTGCCTAGCGTTGCGCCAATAGCATTATCCGCCCTACTCTCCTGCGGACGAGCCATTAA	FGA*RCANSIIRPTLLRTSH*	-1.598	0.028784	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24431	EIDDRRGFNSLNQPNHKSRS			20	SLAY-screened peptide P2781			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGATTGATGATCGCCGTGGGTTTAATTCGCTCAACCAGCCGAATCACAAGTCCCGTTCCTAA	EIDDRRGFNSLNQPNHKSRS*	-1.598	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24432	IIKAFLETMYNRNMAHCNPA			20	SLAY-screened peptide P2782			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTATTAAGGCCTTCCTTGAGACTATGTACAATCGGAATATGGCTCATTGCAACCCTGCTTAA	IIKAFLETMYNRNMAHCNPA*	-1.598	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24433	RACINASTTYCTKSS			15	SLAY-screened peptide P2783			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCTTGCATTAATGCGTCGACTACTTACTGTACCAAGTCGTCGTAGTTCCATTATTCTTAA	RACINASTTYCTKSS*FHYS*	-1.597	0.00137	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24434	PSACINPNQN			10	SLAY-screened peptide P2784			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCCGCCTGTATTAATCCTAATCAGAATTAGGCTACCAATTGCCGGTTCAAGGGTGTGTAA	PSACINPNQN*ATNCRFKGV*	-1.597	0.000298	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24435	LL			2	SLAY-screened peptide P2785			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCTTTAGCATAGGCCGCCCATCGTCGTTGTAATGCTGTTTATTGTAGGTCCAAGATCTAAC	LL*HRPPIVVVMLFIVGPRSN	-1.597	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24436	LCPTSTVDRHYDYNVPMWNE			20	SLAY-screened peptide P2786			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTGCCCGACGTCGACCGTTGATAGGCACTATGACTACAATGTCCCTATGTGGAACGAGTAA	LCPTSTVDRHYDYNVPMWNE*	-1.596	0.006591	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24437	RCSPVFIYFPHFDAVSNVLV			20	SLAY-screened peptide P2787			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTGTAGCCCCGTGTTCATTTATTTTCCCCATTTCGACGCGGTTTCTAACGTTCTCGTTTAA	RCSPVFIYFPHFDAVSNVLV*	-1.596	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24438	HYMFPTVFARSNISHPHPPN			20	SLAY-screened peptide P2788			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTACATGTTCCCCACTGTCTTTGCCCGTTCCAATATTTCGCATCCCCATCCTCCGAACTAA	HYMFPTVFARSNISHPHPPN*	-1.596	0.016863	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24439	TKANANLYPPPNITLPMYMR			20	SLAY-screened peptide P2789			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGAAGGCCAATGCTAATCTCTACCCTCCTCCTAATATTACGTTGCCCATGTACATGCGCTAA	TKANANLYPPPNITLPMYMR*	-1.596	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24440	TVRTPTNCVHRLACPAV			17	SLAY-screened peptide P2790			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGTTCGTACCCCCACTAATTGTGTCCATCGTTTGGCTTGCCCGGCTGTTTAGAGTACTTAA	TVRTPTNCVHRLACPAV*ST*	-1.595	0.003792	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24441	YA			2	SLAY-screened peptide P2791			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGCCTAGACGCAGTTGCTTACGTGTACCTCTAGCCATACCACCGAGAACGCTAAGTACTAA	YA*TQLLTCTSSHTTENAKY*	-1.595	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24442	PVIIIIHESGNV			12	SLAY-screened peptide P2792			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGTTATCATCATTATTCACGAGTCCGGTAATGTTTAGGCCTGCACGGTCTATGTTTCCTAA	PVIIIIHESGNV*ACTVYVS*	-1.595	0.000979	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24443	FTHYHI			6	SLAY-screened peptide P2793			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTACTCACTACCATATTTAGAAGACCTCTTCTTTTGAGCCCCGTCTCGGGACGAGTAACTAA	FTHYHI*KTSSFEPRLGTSN*	-1.595	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24444	IWHRILMSLSPAARLLRGRSN			21	SLAY-screened peptide P2794			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTGGCATCGAATACTCATGAGCTTAAGCCCAGCCGCACGACTACTGCGCGGTCGGTCTAAC	IWHRILMSLSPAARLLRGRSN	-1.595	0.024526	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24445	PSWGCWDHFHAWANLPINIT			20	SLAY-screened peptide P2795			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCCTGGGGCTGTTGGGATCACTTCCATGCTTGGGCTAACTTGCCTATCAATATCACCTAA	PSWGCWDHFHAWANLPINIT*	-1.595	0.010987	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24446	TLKCQRYQFVQIRKLSNPYI			20	SLAY-screened peptide P2796			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTTGAAGTGCCAGCGGTATCAGTTCGTCCAGATCAGGAAGTTGAGCAACCCTTACATTTAA	TLKCQRYQFVQIRKLSNPYI*	-1.595	0.006175	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24447	LTWRPSFRNRFLWNVMDPCR			20	SLAY-screened peptide P2797			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCACCTGGCGCCCCTCGTTCCGCAACCGCTTCCTGTGGAACGTCATGGACCCTTGTCGCTAA	LTWRPSFRNRFLWNVMDPCR*	-1.594	0.000106	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24448	LVQLPTPLITRGLFHLNL			18	SLAY-screened peptide P2798			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGTCCAGCTCCCGACTCCGCTTATCACTCGTGGCCTGTTTCACCTCAACCTGTAGGCGTAA	LVQLPTPLITRGLFHLNL*A*	-1.594	0.000516	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24449	LKSRRAPCVSIYPRCRYYYF			20	SLAY-screened peptide P2799			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAAGTCTCGCCGCGCGCCCTGCGTCTCCATCTACCCTCGGTGTCGCTATTATTACTTCTAA	LKSRRAPCVSIYPRCRYYYF*	-1.594	0.008347	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24450	PALPMMLRNYYTVMMECHMM			20	SLAY-screened peptide P2800			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGCCCTCCCTATGATGCTCCGTAATTACTATACTGTCATGATGGAGTGTCACATGATGTAA	PALPMMLRNYYTVMMECHMM*	-1.594	0.036681	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24451	FLNNLNKNCPSNRSHNAWPY			20	SLAY-screened peptide P2801			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCTTAATAATCTTAATAAGAATTGTCCTTCGAATCGTTCTCATAATGCTTGGCCTTATTAA	FLNNLNKNCPSNRSHNAWPY*	-1.594	0.004408	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24452	IQGLSTLKV			9	SLAY-screened peptide P2802			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCAGGGTCTGTCGACTCTTAAGGTCTAGCCGTACCGCCTTGCGGGCGCCGCCTATTGTTAA	IQGLSTLKV*PYRLAGAAYC*	-1.593	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24453	SCGITTNRRAHITDSHARWN			20	SLAY-screened peptide P2803			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTGCGGTATTACGACCAACAGGCGTGCTCATATTACGGATAGCCATGCCAGGTGGAATTAA	SCGITTNRRAHITDSHARWN*	-1.593	0.007256	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24454	LPLRSCHQL			9	SLAY-screened peptide P2804			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCGCTTCGCAGCTGTCATCAGCTTTAGATCCTCAACACCCTTAACCTTACCTCCAACTAA	LPLRSCHQL*ILNTLNLTSN*	-1.592	0.002019	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24455	ENHMDRADLTDVPSFTCGLL			20	SLAY-screened peptide P2805			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGAACCATATGGACCGGGCCGATCTCACCGACGTTCCCTCTTTTACGTGCGGCTTGCTGTAA	ENHMDRADLTDVPSFTCGLL*	-1.592	0.030523	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24456	GYPG			4	SLAY-screened peptide P2806			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTATCCGGGCTAGTTCTCCTATACGCTTTCCGTTCAGTGTATTCTTAGCGTTGGGATCTAA	GYPG*FSYTLSVQCILSVGI*	-1.592	0.000312	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24457	VAHYTPSPFTYLPASTMYTN			20	SLAY-screened peptide P2807			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGCGCACTACACTCCGTCGCCTTTTACTTACCTGCCGGCTAGTACTATGTATACCAATTAA	VAHYTPSPFTYLPASTMYTN*	-1.591	0.006409	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24458	PQRGRAFGLRMPSMLAPSVFN			21	SLAY-screened peptide P2808			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCAGAGGGGACGAGCTTTTGGTCTTAGAATGCCATCGATGCTAGCCCCATCTGTATTTAAC	PQRGRAFGLRMPSMLAPSVFN	-1.591	0.015781	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24459	SRGTPSNAIYFHLLRDQSLN			20	SLAY-screened peptide P2809			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCGTGGTACGCCCAGTAACGCCATCTATTTTCACCTTTTGCGTGACCAGTCCCTCAACTAA	SRGTPSNAIYFHLLRDQSLN*	-1.591	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24460	AHD			3	SLAY-screened peptide P2810			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCATGATTAGCTCACGACTCACAATTACCAGTACACTCGTTACATTTATTGTCATTATTAA	AHD*LTTHNYQYTRYIYCHY*	-1.591	0.000177	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24461	TRFWQICTNSNLRTLRWNRP			20	SLAY-screened peptide P2811			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCGGTTTTGGCAGATCTGCACGAATAGTAACCTTCGTACCCTGCGGTGGAACCGCCCCTAA	TRFWQICTNSNLRTLRWNRP*	-1.591	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24462	RPTA			4	SLAY-screened peptide P2812			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCCACGGCGTAGTCCACCGTTCGGGCCGCGCAGCAGAACCTCCATCCCTCTCACCCGTAA	RPTA*STVRAAQQNLHPSHP*	-1.591	0.016473	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24463	SKRRFVSTTCPRNSHISDST			20	SLAY-screened peptide P2813			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAAGCGCCGCTTTGTCAGCACGACCTGCCCCCGCAACTCTCATATTTCGGATTCCACGTAA	SKRRFVSTTCPRNSHISDST*	-1.59	0.018586	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24464	LQYRRHSHLFKGVGLSPSTY			20	SLAY-screened peptide P2814			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCAGTACCGTCGCCACTCCCACTTGTTTAAGGGTGTTGGCTTGTCCCCCTCTACTTACTAA	LQYRRHSHLFKGVGLSPSTY*	-1.59	0.002677	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24465	NAATDDTLTLRWPSPRPSNT			20	SLAY-screened peptide P2815			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGCCGCCACGGACGATACGCTTACGCTCCGTTGGCCCTCGCCTCGCCCTTCCAATACGTAA	NAATDDTLTLRWPSPRPSNT*	-1.59	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24466	PFYSETPILTHSCNTLYFAM			20	SLAY-screened peptide P2816			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTTCTATTCCGAGACCCCCATTTTGACTCACAGTTGCAATACCTTGTATTTCGCCATGTAA	PFYSETPILTHSCNTLYFAM*	-1.59	0.026751	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24467	HHMTYTDVTNAKCTRLMVVT			20	SLAY-screened peptide P2817			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCACATGACCTATACTGATGTTACTAATGCCAAGTGCACGCGTTTGATGGTCGTCACCTAA	HHMTYTDVTNAKCTRLMVVT*	-1.59	0.032556	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24468	SWTTPKGLSCRPGRSYPYS			19	SLAY-screened peptide P2818			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTGGACCACTCCCAAGGGCCTGTCGTGCCGCCCGGGCCGCTCTTATCCGTATAGTTAGTAA	SWTTPKGLSCRPGRSYPYS**	-1.59	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24469	CPTDATLTPISHNS			14	SLAY-screened peptide P2819			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCCACTGATGCGACTCTCACTCCCATTAGCCATAATTCTTAGATGAATTGGCCTATCTAA	CPTDATLTPISHNS*MNWPI*	-1.59	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24470	PIGHIWNGSTIVFHNCRFGS			20	SLAY-screened peptide P2820			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATTGGCCATATCTGGAATGGTTCTACTATCGTCTTTCATAACTGCCGCTTTGGGAGTTAA	PIGHIWNGSTIVFHNCRFGS*	-1.589	0.001867	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24471	VHPSHGSSSTAHNYTAPRTT			20	SLAY-screened peptide P2821			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCACCCGAGTCATGGTAGCTCTTCGACGGCGCACAATTATACCGCTCCGCGGACTACTTAA	VHPSHGSSSTAHNYTAPRTT*	-1.589	0.008466	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24472	FMPVLLILFPLTCPCTRHQS			20	SLAY-screened peptide P2822			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTATGCCGGTGCTCCTTATCCTCTTTCCCCTGACCTGTCCTTGCACGCGCCATCAGAGTTAA	FMPVLLILFPLTCPCTRHQS*	-1.588	0.000482	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24473	NFYIAEPRKCHGFLLNMRGS			20	SLAY-screened peptide P2823			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTTTTACATCGCGGAGCCTCGCAAGTGTCATGGTTTCCTCCTCAATATGCGTGGCTCTTAA	NFYIAEPRKCHGFLLNMRGS*	-1.588	0.001563	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24474	SKSPPNYCCP			10	SLAY-screened peptide P2824			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCAAGTCGCCTCCGAACTACTGTTGCCCCTAGTACCGCGACGCGAATGACCTGTTGAGGTAA	SKSPPNYCCP*YRDANDLLR*	-1.588	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24475	QDGYTSSEDQSSIYRHNLKN			20	SLAY-screened peptide P2825			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGACGGTTACACGTCTAGTGAGGATCAGTCTAGTATTTACCGCCACAACCTTAAGAATTAA	QDGYTSSEDQSSIYRHNLKN*	-1.588	0.024643	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24476	QNK			3	SLAY-screened peptide P2826			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGAACAAGTAGTATTGCACTTATGCGATTTTGTTTAACATTTATAACCACATACTTAGTAAC	QNK*YCTYAILFNIYNHILSN	-1.588	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24477	PLRDFLIRRLIGVTSRRHNT			20	SLAY-screened peptide P2827			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCCGTGACTTCCTTATTCGGCGCCTTATTGGCGTGACCAGTAGGCGGCACAATACTTAA	PLRDFLIRRLIGVTSRRHNT*	-1.588	0.003921	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24478	TGLGGLTDYNLLAITYDCLL			20	SLAY-screened peptide P2828			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGGTCTTGGGGGGCTTACTGACTACAACCTGCTCGCTATTACTTACGACTGTTTGCTGTAA	TGLGGLTDYNLLAITYDCLL*	-1.587	0.020983	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24479	FNKWPVCSLHPGRCVNLMTP			20	SLAY-screened peptide P2829			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTAACAAGTGGCCCGTCTGTTCGTTGCATCCTGGCCGTTGTGTGAACCTCATGACCCCTTAA	FNKWPVCSLHPGRCVNLMTP*	-1.587	0.046316	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24480	SALALCPSVSLSVE			14	SLAY-screened peptide P2830			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGCACTTGCTTTATGCCCATCGGTGTCCCTGTCAGTTGAGTGACTATTATGCTTATTAACT	SALALCPSVSLSVE*LLCLLT	-1.587	0.0001	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24481	FAARVVVCGPTNNTAVSIRI			20	SLAY-screened peptide P2831			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGCGGCGCGCGTGGTCGTCTGTGGTCCCACCAACAACACCGCCGTCTCCATTCGGATCTAA	FAARVVVCGPTNNTAVSIRI*	-1.587	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24482	IMTMSEYSFNSSVHSNHYYLT			21	SLAY-screened peptide P2832			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTATGACTATGTCGGAGTATTCGTTCAACAGTTCCGTCCATTCGAACCACTATTATTTAACT	IMTMSEYSFNSSVHSNHYYLT	-1.587	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24483	KPAAEPPIVNGGNNYSRQYS			20	SLAY-screened peptide P2833			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCCGGCTGCTGAGCCGCCCATTGTGAATGGCGGTAACAACTACTCGCGGCAGTATTCTTAA	KPAAEPPIVNGGNNYSRQYS*	-1.586	0.000179	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24484	MLWWTHKYYDWLA			13	SLAY-screened peptide P2834			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCTGTGGTGGACCCATAAGTATTATGATTGGCTTGCGTAGTCCTACCGTGGCCGCTAGTAA	MLWWTHKYYDWLA*SYRGR**	-1.586	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24485	NDATIGGETVSPRVHGNNSI			20	SLAY-screened peptide P2835			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGACGCCACCATTGGGGGCGAGACTGTTTCGCCTAGGGTTCATGGGAATAACAGTATTTAA	NDATIGGETVSPRVHGNNSI*	-1.586	0.02355	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24486	PWCPASQINHFPNNRGATST			20	SLAY-screened peptide P2836			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGGTGTCCTGCGTCTCAGATTAATCACTTTCCTAATAACAGGGGCGCTACCTCCACCTAA	PWCPASQINHFPNNRGATST*	-1.586	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24487	INPIDRHTCDNPLYYIISHF			20	SLAY-screened peptide P2837			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAATCCGATTGACAGGCACACCTGCGATAATCCCCTTTACTACATTATCAGTCACTTCTAA	INPIDRHTCDNPLYYIISHF*	-1.585	0.011167	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24488	ITIFTRSDFNHRTSYALIPA			20	SLAY-screened peptide P2838			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTACCATTTTCACTCGCTCCGATTTCAATCATCGCACTAGTTACGCTCTCATCCCCGCCTAA	ITIFTRSDFNHRTSYALIPA*	-1.585	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24489	QRVCPKAPRIRAPPHTLHTW			20	SLAY-screened peptide P2839			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCGGGTCTGTCCGAAGGCTCCGCGTATCCGTGCCCCGCCTCACACTCTGCACACTTGGTAA	QRVCPKAPRIRAPPHTLHTW*	-1.585	0.009614	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24490	YLSGLRRFYYIWRYLGKTIS			20	SLAY-screened peptide P2840			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCTCAGCGGCCTTAGGCGGTTTTATTATATCTGGCGTTACCTCGGGAAGACTATCAGTTAA	YLSGLRRFYYIWRYLGKTIS*	-1.585	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24491	SSRTSGTIENTRHPLTIIVR			20	SLAY-screened peptide P2841			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTCGCGTACCTCGGGTACTATTGAGAACACTCGTCATCCCTTGACCATTATTGTCAGGTAA	SSRTSGTIENTRHPLTIIVR*	-1.585	8.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24492	SSVNLTALFTVNSGNTCSVR			20	SLAY-screened peptide P2842			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTCCGTTAATCTGACTGCCCTGTTCACCGTGAATAGCGGTAACACCTGCTCGGTTCGTTAA	SSVNLTALFTVNSGNTCSVR*	-1.585	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24493	PSRSVNHYDRIDFCIHRAII			20	SLAY-screened peptide P2843			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCGCGCTCCGTTAACCATTACGATCGTATCGACTTCTGCATCCACCGTGCCATTATCTAA	PSRSVNHYDRIDFCIHRAII*	-1.585	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24494	TNRLPETCHHYCVHPRYLKN			20	SLAY-screened peptide P2844			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGAACCGTCTGCCTGAGACCTGTCATCATTACTGTGTCCACCCCCGGTACCTCAAGAATTAA	TNRLPETCHHYCVHPRYLKN*	-1.584	0.010822	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24495	CSPFHTDLFTLL			12	SLAY-screened peptide P2845			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTCGCCCTTTCACACTGACCTTTTTACGCTCCTTTAGCCTGTTCGCCTGAATTGTAATTAA	CSPFHTDLFTLL*PVRLNCN*	-1.584	0.00156	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24496	LSHPAPQPSSDRSNQLEVPY			20	SLAY-screened peptide P2846			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGAGCCACCCTGCCCCTCAGCCTAGCAGCGATCGCTCGAATCAGCTCGAGGTTCCGTATTAA	LSHPAPQPSSDRSNQLEVPY*	-1.584	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24497	CHKTSPKGEHDISYTQTPHH			20	SLAY-screened peptide P2847			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCACAAGACCTCGCCCAAGGGTGAGCACGATATCAGTTACACGCAGACCCCCCACCACTAA	CHKTSPKGEHDISYTQTPHH*	-1.584	0.000255	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24498	SSVCRPFSQFMYAHVNSFAV			20	SLAY-screened peptide P2848			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAGTGTCTGCCGTCCTTTTAGCCAGTTCATGTACGCTCATGTCAATTCCTTCGCTGTCTAA	SSVCRPFSQFMYAHVNSFAV*	-1.584	0.000349	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24499	LS			2	SLAY-screened peptide P2849			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAGCTAGCCTGATTACAATCAGAGTGTGAATTTGTACTTGCTTTTCATGGATGTTTAGTAA	LS*PDYNQSVNLYLLFMDV**	-1.583	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24500	YTYCTFTSTHTVICYFPTKR			20	SLAY-screened peptide P2850			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACGTACTGCACCTTTACCTCTACGCACACTGTGATCTGTTATTTCCCCACGAAGCGTTAA	YTYCTFTSTHTVICYFPTKR*	-1.583	0.019631	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24501	RPVGPMLCHTEVTMLIIRTN			20	SLAY-screened peptide P2851			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCTGTCGGTCCTATGCTGTGCCATACTGAGGTCACTATGCTTATCATTCGCACTAATTAA	RPVGPMLCHTEVTMLIIRTN*	-1.583	0.000971	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24502	LS			2	SLAY-screened peptide P2852			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCTTAGCTTGATGATAAGATCGCGTACACTCCGTAGCTGTTTAATAATCGTGATCCCTAA	LS*LDDKIAYTP*LFNNRDP*	-1.583	0.011105	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24503	SSSRKFRLTSAASAVAPLSN			20	SLAY-screened peptide P2853			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCAGTTCGCGCAAGTTCCGCCTTACGAGCGCGGCCAGTGCCGTGGCCCCGCTGAGCAACTAA	SSSRKFRLTSAASAVAPLSN*	-1.583	0.008045	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24504	CGIEHNNLTLSPFSGSYHIM			20	SLAY-screened peptide P2854			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGGGATTGAGCATAATAATCTTACCCTCTCCCCGTTCAGCGGCTCTTATCATATTATGTAA	CGIEHNNLTLSPFSGSYHIM*	-1.582	0.013323	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24505	HLLASLG			7	SLAY-screened peptide P2855			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCTACTAGCTAGTCTTGGATGAATTAGTCGGCTTTTGGGGTCCTTGACTATGACAGCTAAC	HLLASLG*ISRLLGSLTMTAN	-1.582	0.045915	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24506	PTLEHKNWFNFKSALHTNNR			20	SLAY-screened peptide P2856			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACTCTGGAGCATAAGAATTGGTTTAACTTTAAGTCTGCCTTGCACACTAATAATCGCTAA	PTLEHKNWFNFKSALHTNNR*	-1.582	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24507	TAPSYPHINNHKS			13	SLAY-screened peptide P2857			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGCCCCGTCTTATCCTCATATTAACAATCACAAGAGTTAGGTTCACCACACCATTATCTAA	TAPSYPHINNHKS*VHHTII*	-1.582	0.004712	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24508	LLLQKAPLASSAHTHCNGPAN			21	SLAY-screened peptide P2858			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCTGCTCCAGAAGGCTCCTCTCGCCTCCAGCGCGCACACGCATTGCAACGGGCCCGCTAAC	LLLQKAPLASSAHTHCNGPAN	-1.582	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24509	LVVYLVSLCSTSSTQFVLYL			20	SLAY-screened peptide P2859			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGTGGTCTACCTCGTGTCCCTGTGTTCTACTTCTAGCACCCAGTTCGTGTTGTACCTCTAA	LVVYLVSLCSTSSTQFVLYL*	-1.581	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24510	CRQTHRKAGRPSYS			14	SLAY-screened peptide P2860			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGGCAGACCCATAGGAAGGCGGGGCGGCCGTCCTATTCCTAGCTGCACATCGATAACTAA	CRQTHRKAGRPSYS*LHIDN*	-1.581	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24511	APRMHHVTLYPSRDDKFSGT			20	SLAY-screened peptide P2861			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCCGCGTATGCACCACGTCACCCTTTACCCTTCGCGTGACGATAAGTTTTCCGGGACGTAA	APRMHHVTLYPSRDDKFSGT*	-1.581	0.009841	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24512	TVFLKAASYLSVRFMTCYTV			20	SLAY-screened peptide P2862			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGTCTTCCTTAAGGCTGCTAGCTATTTGTCTGTTCGGTTTATGACTTGCTATACCGTTTAA	TVFLKAASYLSVRFMTCYTV*	-1.581	0.014234	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24513	LVEHMMSKFNNLRNYLRAIS			20	SLAY-screened peptide P2863			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGTGGAGCATATGATGAGCAAGTTCAATAATCTGAGGAATTATTTGCGGGCGATTTCTTAA	LVEHMMSKFNNLRNYLRAIS*	-1.581	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24514	PVFANSNSFQSWRRNPGFHC			20	SLAY-screened peptide P2864			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGTCTTTGCCAACAGTAATAGCTTTCAGAGCTGGCGCCGCAATCCTGGCTTTCACTGCTAA	PVFANSNSFQSWRRNPGFHC*	-1.581	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24515	TPPNYCAAGFCCPQFPIIGV			20	SLAY-screened peptide P2865			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCTCCTAATTACTGCGCGGCCGGCTTCTGTTGCCCTCAGTTTCCGATTATCGGCGTGTAA	TPPNYCAAGFCCPQFPIIGV*	-1.58	0.03764	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24516	RGPPVTSIALNLLRSTIACSN			21	SLAY-screened peptide P2866			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGGTCCGCCCGTCACTTCCATTGCCTTGAATCTTCTCAGATCCACAATTGCGTGCTCTAAC	RGPPVTSIALNLLRSTIACSN	-1.58	0.003196	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24517	HTCTTNPPADLH			12	SLAY-screened peptide P2867			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACCTGTACCACTAACCCTCCCGCCGACCTTCACTAGCATCGTAGTTGTACGCCTGCTTAA	HTCTTNPPADLH*HRSCTPA*	-1.58	4.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24518	ILCIYRPRRLPSFRIYLGLR			20	SLAY-screened peptide P2868			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCTGTGCATTTACCGTCCCCGGCGTTTGCCTTCCTTTCGCATCTATCTGGGTCTTCGTTAA	ILCIYRPRRLPSFRIYLGLR*	-1.58	0.000292	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24519	YLTIYSLPFAMSITNVTLIH			20	SLAY-screened peptide P2869			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCTTACCATTTATTCTTTGCCCTTTGCGATGTCGATTACTAATGTTACCCTGATTCACTAA	YLTIYSLPFAMSITNVTLIH*	-1.58	0.000395	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24520	RTSVITNLLVHRYYSTLMPL			20	SLAY-screened peptide P2870			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACTTCTGTCATTACCAACCTCCTTGTTCATAGGTATTATTCCACGTTGATGCCGCTCTAA	RTSVITNLLVHRYYSTLMPL*	-1.58	0.001705	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24521	RYPPASHPRQAVAYSHSRSM			20	SLAY-screened peptide P2871			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTACCCCCCCGCTTCTCATCCGCGCCAGGCCGTCGCTTATTCCCACAGCAGGAGTATGTAA	RYPPASHPRQAVAYSHSRSM*	-1.58	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24522	ITYGHLACPSVNRRFLTAST			20	SLAY-screened peptide P2872			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTACGTACGGCCACCTCGCGTGTCCCTCTGTCAATCGTCGGTTCCTCACCGCCTCCACCTAA	ITYGHLACPSVNRRFLTAST*	-1.579	7.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24523	FYALHNHKFIATNKVKTNDS			20	SLAY-screened peptide P2873			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTATGCTCTGCACAACCATAAGTTCATCGCTACCAATAAGGTGAAGACCAATGATAGTTAA	FYALHNHKFIATNKVKTNDS*	-1.579	0.000872	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24524	QRTYRASINFGHSYVCYSPF			20	SLAY-screened peptide P2874			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCGTACTTACAGGGCGTCCATCAATTTTGGTCACAGCTATGTGTGCTATTCGCCCTTCTAA	QRTYRASINFGHSYVCYSPF*	-1.579	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24525	GEHAY			5	SLAY-screened peptide P2875			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTGAGCACGCGTACTAGCCTGAGCACTCGTCGAACGTTCAGAAGCGGCTTAGTAGGAGTTAA	GEHAY*PEHSSNVQKRLSRS*	-1.579	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24526	TSTTFRQPNCTVSLARATLS			20	SLAY-screened peptide P2876			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCTACTACGTTCCGTCAGCCTAATTGCACCGTTAGTCTGGCCCGCGCTACGCTGTCTTAA	TSTTFRQPNCTVSLARATLS*	-1.578	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24527	HNIAYTKKRAGPLRVLCVGSN			21	SLAY-screened peptide P2877			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAACATTGCCTACACTAAGAAGCGAGCAGGGCCTTTGCGGGTCCTTTGTGTGGGCAGTAAC	HNIAYTKKRAGPLRVLCVGSN	-1.578	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24528	NISHISKNHS			10	SLAY-screened peptide P2878			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATATCAGTCATATCTCCAAGAATCATTCCTAGATCTGTTGTAGCGGGTTTCCTAACTCCTAA	NISHISKNHS*ICCSGFPNS*	-1.578	0.004037	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24529	RLRPCDSNSTWWSLNSID			18	SLAY-screened peptide P2879			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCTCCGGCCTTGTGACTCTAATAGCACTTGGTGGAGCCTTAATTCTATCGACTAGGACTAA	RLRPCDSNSTWWSLNSID*D*	-1.578	0.001922	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24530	TGPYHPLRIIPCSNLYYGIL			20	SLAY-screened peptide P2880			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGGCCCGTATCACCCCCTGAGGATCATCCCCTGTTCGAACCTTTACTACGGGATTCTTTAA	TGPYHPLRIIPCSNLYYGIL*	-1.577	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24531	ATPSGVWPATVLDQSMSPNF			20	SLAY-screened peptide P2881			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGACCCCGTCTGGTGTGTGGCCTGCGACCGTGCTTGATCAGTCCATGTCTCCCAACTTTTAA	ATPSGVWPATVLDQSMSPNF*	-1.577	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24532	YWDHHCDNDSRINRVHMYLL			20	SLAY-screened peptide P2882			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTGGGACCACCATTGTGATAACGACAGCCGTATCAACAGGGTTCATATGTATCTTCTGTAA	YWDHHCDNDSRINRVHMYLL*	-1.577	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24533	CTHAHQRAISTYTI			14	SLAY-screened peptide P2883			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCACGCATGCCCATCAGCGGGCCATCTCGACTTATACCATATGACCACTCTTCTGCTGTAAC	CTHAHQRAISTYTI*PLFCCN	-1.577	0.000349	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24534	PLPLLYSPPYYSQIIPCSLL			20	SLAY-screened peptide P2884			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTGCCCTTGCTGTATTCGCCCCCCTACTACTCCCAGATCATTCCCTGTAGCCTTCTCTAA	PLPLLYSPPYYSQIIPCSLL*	-1.576	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24535	GTNWSTP			7	SLAY-screened peptide P2885			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGACCAACTGGTCTACCCCGTAGTTCAGGGCTCACCCTTATTCTAACACTACTAACCCGTAA	GTNWSTP*FRAHPYSNTTNP*	-1.576	0.026293	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24536	TQCPPYLTCYVPSIVPYCWV			20	SLAY-screened peptide P2886			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCAGTGCCCCCCGTATCTCACCTGCTATGTGCCTAGTATTGTCCCTTACTGCTGGGTGTAA	TQCPPYLTCYVPSIVPYCWV*	-1.576	0.027274	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24537	LNRPFNPWVPCFMV			14	SLAY-screened peptide P2887			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAATCGCCCGTTCAATCCTTGGGTTCCCTGTTTTATGGTTTAGCTTTATTGCAGTGACTAA	LNRPFNPWVPCFMV*LYCSD*	-1.576	0.016877	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24538	HSGRDSMFDD			10	SLAY-screened peptide P2888			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTCCGGCCGCGATTCCATGTTTGACGACTAGATTGACGTGTCTATCGCTCACGTTGGTAAC	HSGRDSMFDD*IDVSIAHVGN	-1.576	0.000112	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24539	CCPFFTNSPYDHPCIVYARA			20	SLAY-screened peptide P2889			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTGCCCCTTTTTTACCAACTCTCCTTACGATCACCCTTGCATTGTTTACGCTCGTGCCTAA	CCPFFTNSPYDHPCIVYARA*	-1.576	0.007485	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24540	PTKPPTPTHLILTYPC			16	SLAY-screened peptide P2890			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACCAAGCCGCCTACGCCCACCCACCTGATTCTTACCTATCCCTGTTAGAATGTTTGTTAA	PTKPPTPTHLILTYPC*NVC*	-1.575	0.005307	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24541	CAPPDIIHLFNATGSNFPEL			20	SLAY-screened peptide P2891			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGCGCCTCCCGATATTATCCATCTGTTCAACGCTACTGGCAGCAATTTTCCGGAGCTTTAA	CAPPDIIHLFNATGSNFPEL*	-1.575	2.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24542	NYITAQHAVSERPVRVMLHT			20	SLAY-screened peptide P2892			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTACATCACCGCCCAGCATGCTGTTAGCGAGCGCCCGGTTCGGGTTATGCTTCATACGTAA	NYITAQHAVSERPVRVMLHT*	-1.574	0.002355	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24543	PSIKCYDQPMHNRPLIYYSA			20	SLAY-screened peptide P2893			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGTATCAAGTGTTATGACCAGCCCATGCATAACCGCCCCCTTATTTACTACAGTGCCTAA	PSIKCYDQPMHNRPLIYYSA*	-1.574	0.001006	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24544	LVPIWRRCSRRSAVNTCISL			20	SLAY-screened peptide P2894			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGTCCCCATTTGGCGTCGTTGTTCCCGTCGCTCTGCTGTCAATACCTGCATTTCTCTGTAG	LVPIWRRCSRRSAVNTCISL*	-1.574	6.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24545	VFPMNDTSHHHLRPVPGGWL			20	SLAY-screened peptide P2895			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTTTCCGATGAACGATACTTCCCACCATCATCTGCGTCCGGTGCCTGGCGGGTGGCTCTAA	VFPMNDTSHHHLRPVPGGWL*	-1.574	0.000143	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24546	QNFRVPCIHCIVSFLDSFYE			20	SLAY-screened peptide P2896			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGAATTTTCGTGTCCCTTGCATTCATTGCATTGTTAGTTTCCTCGACAGCTTTTATGAGTAA	QNFRVPCIHCIVSFLDSFYE*	-1.574	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24547	CLLS			4	SLAY-screened peptide P2897			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTGCTGTCGTAGTCCTCTTCGAACGGGTGTCGTAACTTCCGCCTCCCTCCCATTCTTTAA	CLLS*SSSNGCRNFRLPPIL*	-1.574	0.000106	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24548	DPISSIYASQVRLRTVLGSSN			21	SLAY-screened peptide P2898			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCCCATTAGCAGCATTTACGCCAGCCAGGTACGATTGCGGACCGTCTTGGGGTCCAGTAAC	DPISSIYASQVRLRTVLGSSN	-1.574	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24549	GMCSAYRTYLV			11	SLAY-screened peptide P2899			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCATGTGTAGTGCTTATAGGACCTACCTGGTGTAGAATGGCGTTCCCTTGACCATTAAGTAA	GMCSAYRTYLV*NGVPLTIK*	-1.573	0.000269	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24550	LTATRPYRPP			10	SLAY-screened peptide P2900			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGACCGCGACGCGCCCGTATCGCCCGCCGTAGCGCAACAGCGTTAGGGTTAAGTACACTTAA	LTATRPYRPP*RNSVRVKYT*	-1.573	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24551	LYRNSVLLKASL			12	SLAY-screened peptide P2901			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTATCGGAATAGCGTCCTTCTTAAGGCTAGCTTGTAGTGGCACTTTTGTTATATCGGCTAA	LYRNSVLLKASL*WHFCYIG*	-1.573	0.026418	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24552	SFNAYSRVYSDCYYIHGVAG			20	SLAY-screened peptide P2902			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTTCAATGCTTATTCGCGTGTGTACTCCGATTGCTACTATATCCACGGCGTCGCTGGCTAA	SFNAYSRVYSDCYYIHGVAG*	-1.573	0.022601	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24553	IRNTFLLRRDRMPGAYDSLP			20	SLAY-screened peptide P2903			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAGGAATACGTTCCTTTTGCGGCGGGATCGCATGCCGGGGGCCTATGACTCTCTTCCGTAA	IRNTFLLRRDRMPGAYDSLP*	-1.573	0.003593	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24554	SLKPTGTTRACFQPVISLA			19	SLAY-screened peptide P2904			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCTCAAGCCTACGGGTACGACTCGGGCTTGCTTCCAGCCCGTCATTAGCCTGGCGTAGTAA	SLKPTGTTRACFQPVISLA**	-1.573	0.000465	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24555	VAPGAGFPIRDMVHSRTSHI			20	SLAY-screened peptide P2905			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGGCCCCTGGGGCCGGGTTTCCCATTCGGGATATGGTTCACTCCCGCACCTCCCATATTTAA	VAPGAGFPIRDMVHSRTSHI*	-1.573	0.004701	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24556	RSRGVINKTYSRNAHVHFRN			20	SLAY-screened peptide P2906			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTCGCGCGGGGTTATTAACAAGACGTATTCGCGCAACGCCCATGTTCACTTTCGCAATTAA	RSRGVINKTYSRNAHVHFRN*	-1.573	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24557	SRPDGARHNFHSRPIL			16	SLAY-screened peptide P2907			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCGGCCTGACGGGGCCCGCCATAATTTCCATAGTCGCCCAATACTCTGAGTAAGTCGACCT	SRPDGARHNFHSRPIL*VSRP	-1.573	0.008011	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24558	TVTLGRAIPLPANNP			15	SLAY-screened peptide P2908			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTGACGCTCGGCCGTGCCATTCCCCTTCCTGCCAACAACCCTTAGGATTGCTACCTGTAA	TVTLGRAIPLPANNP*DCYL*	-1.573	0.023582	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24559	PCLMCQSYAPGVGCIFLATL			20	SLAY-screened peptide P2909			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGCCTCATGTGTCAGTCCTACGCCCCCGGTGTTGGTTGCATTTTCCTGGCCACCCTGTAA	PCLMCQSYAPGVGCIFLATL*	-1.572	0.035008	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24560	CEWDLPCRRRAHTDRKAGNP			20	SLAY-screened peptide P2910			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGAGTGGGACCTGCCTTGTCGGCGTCGTGCTCATACCGACCGCAAGGCGGGTAACCCTTAA	CEWDLPCRRRAHTDRKAGNP*	-1.572	0.022776	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24561	IGGYSPSDATYTIVCFMPNGT			21	SLAY-screened peptide P2911			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGGAGGATACAGCCCGTCCGATGCTACTTACACTATTGTTTGCTTTATGCCCAACGGTACG	IGGYSPSDATYTIVCFMPNGT	-1.572	0.026346	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24562	TLKKIKRVVHYLCDWCNHHW			20	SLAY-screened peptide P2912			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTCAAGAAGATCAAGCGCGTTGTGCACTACTTGTGCGACTGGTGTAACCACCATTGGTAA	TLKKIKRVVHYLCDWCNHHW*	-1.572	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24563	CFLSSLIPSRPSLDIGCAHE			20	SLAY-screened peptide P2913			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTTTCTTTCTTCTCTCATCCCTAGCAGGCCCTCCCTGGACATTGGTTGCGCTCATGAGTAA	CFLSSLIPSRPSLDIGCAHE*	-1.572	0.001701	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24564	RAHYIYSTANSL			12	SLAY-screened peptide P2914			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGGCCCACTATATTTACTCCACCGCCAACAGTCTCTAGTTTACTTTCGAGACGTTGGACTAA	RAHYIYSTANSL*FTFETLD*	-1.572	0.007449	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24565	PNTSIVLCHLSRAIYLYRLP			20	SLAY-screened peptide P2915			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAACACTTCCATCGTTCTCTGTCACCTCTCTCGCGCGATTTATTTGTACCGCCTTCCTTAA	PNTSIVLCHLSRAIYLYRLP*	-1.572	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24566	LTSHCQDADCHDMHEPAINI			20	SLAY-screened peptide P2916			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACGTCGCACTGCCAGGATGCTGACTGCCATGATATGCATGAGCCCGCCATTAACATTTAA	LTSHCQDADCHDMHEPAINI*	-1.571	0.024421	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24567	WPGSFRCYPSMHDLSLNPHP			20	SLAY-screened peptide P2917			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCCTGGCAGTTTTCGTTGCTACCCGAGCATGCACGACCTCTCTCTTAACCCCCATCCCTAA	WPGSFRCYPSMHDLSLNPHP*	-1.571	2.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24568	PISKMAINATWIGHDDRWTY			20	SLAY-screened peptide P2918			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATTTCTAAGATGGCTATTAATGCGACTTGGATCGGGCATGACGACCGCTGGACCTACTAA	PISKMAINATWIGHDDRWTY*	-1.571	0.001038	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24569	PPIPVALQCDVHPFVVTPR			19	SLAY-screened peptide P2919			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCGATCCCTGTGGCGCTGCAGTGCGACGTTCACCCTTTCGTGGTCACCCCCCGCTAGTGT	PPIPVALQCDVHPFVVTPR*C	-1.57	0.023995	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24570	CSRFVLAARTYTFEDAT			17	SLAY-screened peptide P2920			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAGCCGCTTCGTGTTGGCGGCGAGGACGTACACGTTCGAGGATGCTACCTAGTAGTACTAA	CSRFVLAARTYTFEDAT**Y*	-1.57	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24571	RSTAQTYRRLADRYMLSFC			19	SLAY-screened peptide P2921			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCTACCGCCCAGACCTACCGTCGCCTTGCTGATCGCTACATGTTGTCCTTTTGTTAGTAA	RSTAQTYRRLADRYMLSFC**	-1.57	0.024691	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24572	TTYGRCLRLSAAPTFPGWGS			20	SLAY-screened peptide P2922			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTACCTACGGTCGGTGCCTCCGTCTCAGTGCGGCTCCCACCTTTCCTGGCTGGGGTTCGTAA	TTYGRCLRLSAAPTFPGWGS*	-1.57	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24573	PKPACIH			7	SLAY-screened peptide P2923			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAAGCCCGCGTGTATCCACTAGATGACGCGCCACGTGAACGTGTTGCTGCCGGACTTCTAA	PKPACIH*MTRHVNVLLPDF*	-1.569	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24574	TNLPPRTHNHFFCILNMCYP			20	SLAY-screened peptide P2924			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGAACTTGCCGCCGAGGACCCACAACCACTTCTTCTGCATCCTTAACATGTGTTATCCGTAA	TNLPPRTHNHFFCILNMCYP*	-1.569	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24575	CIHSNHWVEISARSWSSLTRN			21	SLAY-screened peptide P2925			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTATCCACAGTAACCACTGGGTGGAGATAAGTGCCCGCTCTTGGTCCTCTCTGACCCGTAAC	CIHSNHWVEISARSWSSLTRN	-1.568	0.0122	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24576	WGGWRYRVHPYDSTPSRCNL			20	SLAY-screened peptide P2926			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGGGGGGTTGGCGTTATAGGGTTCACCCCTATGACTCCACGCCCTCCCGGTGTAACCTGTAA	WGGWRYRVHPYDSTPSRCNL*	-1.568	0.000257	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24577	HVGPTAPTYQYFCPPIYFST			20	SLAY-screened peptide P2927			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGTTGGCCCTACTGCTCCGACCTATCAGTATTTTTGCCCCCCGATTTACTTCTCCACCTAA	HVGPTAPTYQYFCPPIYFST*	-1.568	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24578	LYCTCPIHTIQCFMTPYLAL			20	SLAY-screened peptide P2928			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTATTGTACTTGCCCTATCCACACCATTCAGTGCTTCATGACCCCGTATTTGGCCCTGTAA	LYCTCPIHTIQCFMTPYLAL*	-1.567	0.001101	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24579	PAAHFSLR			8	SLAY-screened peptide P2929			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCTGCGCATTTTTCGCTTAGGTAGAACTTCCTTAATACGTATTAGTATCCTAATCCCTAA	PAAHFSLR*NFLNTY*YPNP*	-1.567	0.005837	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24580	LSCTLLISNK			10	SLAY-screened peptide P2930			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTCCTGCACTCTTCTCATTTCTAATAAGTAGGTCATTTCTAGGGACCATCCTATTTATTAA	LSCTLLISNK*VISRDHPIY*	-1.567	0.009034	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24581	SRITQVRCQANRTPNSNKAP			20	SLAY-screened peptide P2931			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCGTATCACTCAGGTCCGTTGCCAGGCGAACCGCACCCCCAACTCCAATAAGGCTCCTTAA	SRITQVRCQANRTPNSNKAP*	-1.566	0.000211	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24582	LTTCPYLQNTPNAPEILLISN			21	SLAY-screened peptide P2932			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGACCACCTGCCCTTACCTCCAGAACACCCCCAATGCCCCGGAGATCCTGTTAATCTCTAAC	LTTCPYLQNTPNAPEILLISN	-1.566	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24583	VQRRSCSHTLCLSFFIFYSH			20	SLAY-screened peptide P2933			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCAGCGCAGGTCTTGTTCGCACACCCTCTGCCTTTCGTTCTTCATTTTCTACTCCCACTAA	VQRRSCSHTLCLSFFIFYSH*	-1.566	0.000826	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24584	HTPRVHHIGSSLDLLPTAHT			20	SLAY-screened peptide P2934			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACCCCTCGCGTCCATCACATTGGGTCGTCCCTTGATTTGCTCCCTACGGCGCATACCTAA	HTPRVHHIGSSLDLLPTAHT*	-1.566	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24585	TGAHEYKGGLARP			13	SLAY-screened peptide P2935			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGGCGCTCACGAGTATAAGGGCGGTCTCGCGAGGCCGTAGTTTTGTACTGTCAACATGTAA	TGAHEYKGGLARP*FCTVNM*	-1.566	0.013085	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24586	PVMVHPRGTTHD			12	SLAY-screened peptide P2936			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGTCATGGTCCACCCCAGGGGGACGACGCACGATTAGACCCTTATTACTTTCGGCTAGTAA	PVMVHPRGTTHD*TLITFG**	-1.566	0.000709	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24587	ARFLYCHRLGHPVPLDSTLN			20	SLAY-screened peptide P2937			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGAGGTTTCTTTATTGTCATCGTCTGGGCCACCCTGTTCCTCTTGACTCCACTCTCAATTAA	ARFLYCHRLGHPVPLDSTLN*	-1.565	0.029201	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24588	CYYPEYRRIASRHALYLIPR			20	SLAY-screened peptide P2938			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTACTACCCGGAGTATCGTAGGATCGCCAGTCGCCACGCCCTCTACCTTATTCCCCGCTAA	CYYPEYRRIASRHALYLIPR*	-1.565	0.016209	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24589	QLPPTSLAHTAGGIPYTPPC			20	SLAY-screened peptide P2939			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCTCCCGCCCACTTCTTTGGCCCATACCGCCGGGGGGATCCCCTACACGCCGCCGTGTTAA	QLPPTSLAHTAGGIPYTPPC*	-1.565	0.000539	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24590	YCPAKSDLDYSARPVFHYHM			20	SLAY-screened peptide P2940			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTGCCCGGCCAAGAGCGACCTTGACTATAGTGCTCGGCCTGTCTTCCATTATCATATGTAA	YCPAKSDLDYSARPVFHYHM*	-1.565	0.007056	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24591	PIHRLHSFLTFELSHVGLRD			20	SLAY-screened peptide P2941			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATCCATCGTCTGCATAGTTTCCTGACCTTCGAGCTCAGTCATGTGGGCCTTCGCGACTAA	PIHRLHSFLTFELSHVGLRD*	-1.565	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24592	GRDLYPAGSYGAFFWDYHYL			20	SLAY-screened peptide P2942			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCGTGACTTGTACCCCGCGGGCAGTTACGGTGCCTTTTTTTGGGACTATCACTATCTGTAA	GRDLYPAGSYGAFFWDYHYL*	-1.565	0.049157	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24593	PRHVWRDNLVCIYTSYSTTF			20	SLAY-screened peptide P2943			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGTCATGTCTGGCGTGACAACCTTGTGTGTATTTACACTTCTTACTCGACCACCTTTTAA	PRHVWRDNLVCIYTSYSTTF*	-1.565	3.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24594	CTPNHHDFQLLRASVVSNLP			20	SLAY-screened peptide P2944			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTACGCCTAATCACCACGACTTTCAGTTGCTGAGGGCCTCTGTCGTGAGCAACCTCCCCTAA	CTPNHHDFQLLRASVVSNLP*	-1.564	0.000437	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24595	IVNWKPKILC			10	SLAY-screened peptide P2945			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGTTAACTGGAAGCCCAAGATTTTGTGCTAGGGCCATGACGCGCCGTGTCCCAAGCACTAA	IVNWKPKILC*GHDAPCPKH*	-1.564	0.001243	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24596	DYNACDHSLSQYRL			14	SLAY-screened peptide P2946			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTATAATGCTTGTGATCATTCCCTTAGCCAGTACCGTTTGTAGGTGAATATCTTGGGCTAA	DYNACDHSLSQYRL*VNILG*	-1.563	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24597	SSAEPRNSHNPLNSPRLASL			20	SLAY-screened peptide P2947			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAGTGCCGAGCCTCGTAATAGCCATAATCCTCTGAATAGCCCTCGCTTGGCGTCCCTTTAA	SSAEPRNSHNPLNSPRLASL*	-1.563	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24598	IHDSTRISHSTGNMQAVPRVT			21	SLAY-screened peptide P2948			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCATGATTCCACCCGTATTTCGCATTCTACCGGCAACATGCAGGCCGTCCCACGAGTAACT	IHDSTRISHSTGNMQAVPRVT	-1.563	0.000102	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24599	PWGSTYTMRQPR			12	SLAY-screened peptide P2949			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGGGGCTCTACCTATACCATGCGGCAGCCTAGGTAGAATGTCTCTCACATGTGTGATTAA	PWGSTYTMRQPR*NVSHMCD*	-1.563	0.015768	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24600	FRCNRAISCSDPPKVSSGLI			20	SLAY-screened peptide P2950			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCGTTGCAACAGGGCCATCTCTTGCAGTGATCCCCCGAAGGTGTCCTCGGGGCTCATCTAA	FRCNRAISCSDPPKVSSGLI*	-1.562	0.000507	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24601	KLKATHPLPDMFIISHQHVS			20	SLAY-screened peptide P2951			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCTCAAGGCCACGCACCCTCTGCCCGATATGTTTATCATCTCTCATCAGCACGTTTCTTAA	KLKATHPLPDMFIISHQHVS*	-1.562	0.00138	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24602	TRYMSRNPTFPVMQTTLRKV			20	SLAY-screened peptide P2952			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCGGTACATGAGTCGTAACCCGACGTTCCCCGTTATGCAGACGACCCTGCGTAAGGTTTAA	TRYMSRNPTFPVMQTTLRKV*	-1.562	0.034076	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24603	FNSSPGRRPKQRHHVYVSVR			20	SLAY-screened peptide P2953			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAACTCTTCCCCTGGCCGTCGGCCCAAGCAGCGCCATCACGTCTATGTCTCGGTGCGCTAA	FNSSPGRRPKQRHHVYVSVR*	-1.561	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24604	FRTSSLPALTNHNYACNSTA			20	SLAY-screened peptide P2954			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCGTACGTCTTCCCTGCCTGCCCTCACTAATCACAATTATGCTTGCAATAGTACCGCTTAA	FRTSSLPALTNHNYACNSTA*	-1.561	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24605	IPKCTSDIYREPFTFALTSN			20	SLAY-screened peptide P2955			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCCGAAGTGTACTAGCGATATTTATCGGGAGCCTTTTACTTTTGCCCTTACCTCCAATTAA	IPKCTSDIYREPFTFALTSN*	-1.561	0.00392	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24606	LPRRVSASFSNSRLRANSNY			20	SLAY-screened peptide P2956			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCCAGGAGGGTGAGTGCTAGTTTCTCTAACAGTCGCTTGCGTGCGAATTCGAACTATTAA	LPRRVSASFSNSRLRANSNY*	-1.56	0.007999	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24607	LRPTDQLPISSWSGSPSLVQ			20	SLAY-screened peptide P2957			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCGCCCCACCGATCAGCTCCCGATTTCTTCCTGGAGCGGGAGTCCTAGTCTGGTTCAGTAA	LRPTDQLPISSWSGSPSLVQ*	-1.56	0.002454	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24608	FLRCYDCYNDHTASDNSHVL			20	SLAY-screened peptide P2958			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCTTAGGTGTTACGATTGCTACAATGACCATACTGCGTCTGACAATTCTCACGTCCTTTAA	FLRCYDCYNDHTASDNSHVL*	-1.56	7.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24609	LTYFYTLCTSFRSRITQCVT			20	SLAY-screened peptide P2959			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCACGTACTTCTACACCCTGTGCACGAGTTTCCGCAGTCGGATTACCCAGTGCGTGACTTAA	LTYFYTLCTSFRSRITQCVT*	-1.56	0.005551	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24610	RAPVLPHTYW			10	SLAY-screened peptide P2960			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGGCCCCCGTGCTTCCGCACACCTATTGGTAGGATCTGGAGGGTTCCGTCGTTCAGCATTAA	RAPVLPHTYW*DLEGSVVQH*	-1.56	0.000503	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24611	CDYCPTEDDYPVAT			14	SLAY-screened peptide P2961			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGACTATTGCCCCACGGAGGACGACTACCCGGTTGCGACCTAGAATGTCCATGCGGGGTAA	CDYCPTEDDYPVAT*NVHAG*	-1.559	6.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24612	IFRNVSITLCFYSTGSKLNY			20	SLAY-screened peptide P2962			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTTTAGGAACGTCTCGATTACGCTGTGCTTCTACTCCACCGGTAGCAAGCTGAACTACTAA	IFRNVSITLCFYSTGSKLNY*	-1.558	0.000211	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24613	DCRHPTNNVPFKYFRPLFLY			20	SLAY-screened peptide P2963			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTGCAGGCACCCCACGAACAATGTTCCGTTCAAGTACTTCCGCCCCCTTTTTTTGTATTAA	DCRHPTNNVPFKYFRPLFLY*	-1.558	0.004061	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24614	LPCCLKCLRLTSATDNMALR			20	SLAY-screened peptide P2964			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCCTGTTGCCTGAAGTGTCTGCGGTTGACCAGTGCTACCGACAATATGGCGTTGAGGTAA	LPCCLKCLRLTSATDNMALR*	-1.558	0.001045	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24615	KRTLST			6	SLAY-screened peptide P2965			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCGTACTCTGTCGACCTGACATGAACTACACTGCTAAGTATTTTGGCAACCACGATTAACT	KRTLST*HELHC*VFWQPRLT	-1.558	0.000237	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24616	RPGA			4	SLAY-screened peptide P2966			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCGGGCGCTTAGTACACGCATCTTCATACCGTCACGCCCGCCACGGATGGTCTCACTTAA	RPGA*YTHLHTVTPATDGLT*	-1.558	0.000221	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24617	SALPCGLCTTTVNLHKHAFR			20	SLAY-screened peptide P2967			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCGCTGCCCTGCGGGCTGTGTACCACCACCGTGAATCTTCATAAGCATGCCTTCCGGTAA	SALPCGLCTTTVNLHKHAFR*	-1.558	3.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24618	PCHHLVNKMKKWFVRRRRVR			20	SLAY-screened peptide P2968			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGTCATCATCTCGTTAACAAGATGAAGAAGTGGTTCGTGCGTAGGAGGCGTGTTAGGTAA	PCHHLVNKMKKWFVRRRRVR*	-1.556	0.032006	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24619	PPTGCTTNFHKYNSILSKLI			20	SLAY-screened peptide P2969			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCGACTGGTTGCACCACGAATTTTCACAAGTACAATTCCATTTTGTCCAAGCTGATCTAA	PPTGCTTNFHKYNSILSKLI*	-1.556	0.02429	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24620	SGINQHIHGTWQLHLAGICV			20	SLAY-screened peptide P2970			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGGCATTAACCAGCATATCCATGGCACCTGGCAGCTTCACTTGGCGGGGATCTGTGTTTAA	SGINQHIHGTWQLHLAGICV*	-1.556	0.026431	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24621	HFGQHLFARFH			11	SLAY-screened peptide P2971			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTTCGGTCAGCATCTTTTCGCTCGGTTTCACTAGCGGCGGATGCCCACTAAGACGCAGTAA	HFGQHLFARFH*RRMPTKTQ*	-1.556	2.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24622	RETSGRTQQSRPC			13	SLAY-screened peptide P2972			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGAGACTTCCGGGCGTACGCAGCAGTCCAGGCCTTGCTAGTACAATAATAACATGGCCTAA	RETSGRTQQSRPC*YNNNMA*	-1.556	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24623	FDIIHTNHRKLGLTLVPIIL			20	SLAY-screened peptide P2973			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGACATCATTCACACTAACCATAGGAAGCTTGGTCTGACCCTCGTTCCGATTATCTTGTAA	FDIIHTNHRKLGLTLVPIIL*	-1.555	0.000306	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24624	SINALLLRSIQHWMPISRPS			20	SLAY-screened peptide P2974			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCATCAATGCGCTTTTGCTGCGTAGTATCCAGCACTGGATGCCCATTAGCCGTCCCTCGTAA	SINALLLRSIQHWMPISRPS*	-1.555	0.01328	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24625	VSNDHPYRNGLALIYLNTNL			20	SLAY-screened peptide P2975			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTAGTAATGACCATCCTTATCGTAATGGCCTCGCCCTGATCTACCTCAACACGAACCTTTAA	VSNDHPYRNGLALIYLNTNL*	-1.555	0.002712	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24626	TRAFSHMSRYSLTAVCLLVFN			21	SLAY-screened peptide P2976			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCGCGCTTTTTCTCACATGTCGCGGTACTCCTTAACAGCAGTATGTTTACTTGTATTTAAC	TRAFSHMSRYSLTAVCLLVFN	-1.555	0.006578	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24627	LGVITSFFAMLLPNYMVLLA			20	SLAY-screened peptide P2977			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGGTGTCATCACTAGCTTCTTTGCCATGCTCCTGCCCAATTATATGGTCCTTCTTGCCTAA	LGVITSFFAMLLPNYMVLLA*	-1.554	0.046954	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24628	RRVNQSPTYVMHRFGHNMRS			20	SLAY-screened peptide P2978			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGCGTCAACCAGAGCCCGACTTATGTCATGCACCGCTTTGGTCACAACATGCGCTCTTAA	RRVNQSPTYVMHRFGHNMRS*	-1.554	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24629	HL			2	SLAY-screened peptide P2979			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCTGTAGGATCACAACAGCCGCGCCACTACGAAGATTGTTCTTGACCATCTCCAGGCTTAA	HL*DHNSRATTKIVLDHLQA*	-1.554	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24630	PTYSSNGTSAMVHTNGPYLS			20	SLAY-screened peptide P2980			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACGTATTCGAGTAATGGTACTTCGGCCATGGTGCATACGAATGGCCCTTACCTTTCGTAA	PTYSSNGTSAMVHTNGPYLS*	-1.554	0.013092	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24631	IADILRYVAALRGIHWFPKR			20	SLAY-screened peptide P2981			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGCTGACATCCTGAGGTACGTTGCCGCGCTTCGGGGCATTCACTGGTTCCCGAAGCGTTAA	IADILRYVAALRGIHWFPKR*	-1.554	0.00036	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24632	IRFSSPRIRITDSD			14	SLAY-screened peptide P2982			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCGCTTTTCCTCGCCCCGTATTCGTATCACGGACAGTGATTAGTAGGACAAGTATGATTAA	IRFSSPRIRITDSD**DKYD*	-1.554	3.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24633	LTCIPLLAHIDWLPVEHASS			20	SLAY-screened peptide P2983			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACCTGTATTCCCCTGCTTGCGCATATCGATTGGCTGCCTGTTGAGCACGCGAGTTCTTAA	LTCIPLLAHIDWLPVEHASS*	-1.553	0.033203	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24634	LTFSRCHASLSELFALPMTH			20	SLAY-screened peptide P2984			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGACTTTTTCTCGCTGTCACGCCAGCCTGAGTGAGCTTTTTGCCCTTCCTATGACCCATTAA	LTFSRCHASLSELFALPMTH*	-1.553	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24635	VPHHHICYEDNPIPMLIRRRN			21	SLAY-screened peptide P2985			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCCTCACCATCATATTTGTTATGAGGATAACCCTATCCCGATGCTGATCAGGAGACGTAAC	VPHHHICYEDNPIPMLIRRRN	-1.553	7.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24636	VRTPLCTPSAAHGGLPVFTA			20	SLAY-screened peptide P2986			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCGGACCCCTTTGTGTACCCCTTCTGCGGCCCACGGGGGGCTTCCGGTTTTCACTGCGTAA	VRTPLCTPSAAHGGLPVFTA*	-1.553	0.021291	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24637	SYLFAFEEYPSSMQSFSISS			20	SLAY-screened peptide P2987			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTACCTTTTTGCCTTTGAGGAGTACCCGTCTTCTATGCAGTCCTTTTCTATTTCGTCCTAA	SYLFAFEEYPSSMQSFSISS*	-1.552	0.001367	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24638	AAAANTPDNCFRETDNKH			18	SLAY-screened peptide P2988			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGCCGCTGCTAATACGCCTGATAACTGTTTTCGGGAGACCGATAATAAGCATTGAGCTAAC	AAAANTPDNCFRETDNKH*AN	-1.552	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24639	CLDPFWKTGNINPCWTCNSL			20	SLAY-screened peptide P2989			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCTGGACCCGTTCTGGAAGACCGGTAATATCAATCCCTGCTGGACGTGCAACAGTCTTTAA	CLDPFWKTGNINPCWTCNSL*	-1.552	0.049144	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24640	ALAARSRVTEQTDRTNDIHL			20	SLAY-screened peptide P2990			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCTTGCGGCGCGCAGCCGCGTTACGGAGCAGACGGATCGCACCAACGATATTCATCTTTAA	ALAARSRVTEQTDRTNDIHL*	-1.552	0.008816	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24641	SYSDVY			6	SLAY-screened peptide P2991			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTATTCGGATGTCTACTAGCCTCGCTGTGTCGACTAGGTCCAGTACTCGAAGGTCCGCTAA	SYSDVY*PRCVD*VQYSKVR*	-1.552	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24642	HCRSSGFNKNPDIYTLSARAN			21	SLAY-screened peptide P2992			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGTCGGTCTAGCGGTTTCAACAAGAACCCGGATATCTACACTCTGTCCGCCCGCGCTAAC	HCRSSGFNKNPDIYTLSARAN	-1.552	0.000224	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24643	SALSPAHGARALSTNDQAMS			20	SLAY-screened peptide P2993			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCGTTGTCCCCCGCCCACGGGGCTCGCGCCCTCTCGACTAATGACCAGGCGATGTCGTAA	SALSPAHGARALSTNDQAMS*	-1.552	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24644	LTVSFDDSLSFVELLTYSKY			20	SLAY-screened peptide P2994			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACTGTCTCCTTTGATGATAGTCTTAGTTTCGTTGAGCTCTTGACCTATAGTAAGTACTAA	LTVSFDDSLSFVELLTYSKY*	-1.551	0.002164	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24645	SSRASAPQRSTPISTRYTDV			20	SLAY-screened peptide P2995			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTCGAGGGCGAGCGCCCCTCAGCGTAGCACGCCCATCTCCACTCGCTATACGGACGTGTAA	SSRASAPQRSTPISTRYTDV*	-1.551	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24646	LRQMPLTREFHYVPSVPNNG			20	SLAY-screened peptide P2996			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCGGCAGATGCCTTTGACCAGGGAGTTCCACTACGTCCCGAGCGTTCCTAATAATGGGTAA	LRQMPLTREFHYVPSVPNNG*	-1.551	6.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24647	LYGPPRLVHYYHRWMCLRPP			20	SLAY-screened peptide P2997			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTACGGGCCTCCCAGGCTGGTCCACTACTACCACCGGTGGATGTGCCTTCGCCCTCCGTAA	LYGPPRLVHYYHRWMCLRPP*	-1.551	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24648	PCVAVYYAASAYAPLYHNWS			20	SLAY-screened peptide P2998			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGCGTTGCGGTGTACTACGCGGCCTCCGCCTATGCTCCTCTTTACCACAATTGGTCTTAA	PCVAVYYAASAYAPLYHNWS*	-1.551	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24649	ILYSGTYSCPYAYFLTPTYH			20	SLAY-screened peptide P2999			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCTGTATAGTGGCACCTACTCCTGCCCTTATGCGTACTTCCTCACCCCGACCTACCATTAA	ILYSGTYSCPYAYFLTPTYH*	-1.55	0.023701	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24650	ENHIEIHLVDRSRSASLLSIN			21	SLAY-screened peptide P3000			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGAACCATATTGAGATCCACCTTGTTGACAGATCGAGGTCTGCTTCTCTGCTGTCCATTAAC	ENHIEIHLVDRSRSASLLSIN	-1.55	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24651	HLLHLLISRFLNLQHGLDDR			20	SLAY-screened peptide P3001			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTTGCTCCACCTCCTCATTAGCCGCTTCCTCAACTTGCAGCACGGTTTGGATGATAGGTAA	HLLHLLISRFLNLQHGLDDR*	-1.55	0.012282	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24652	SATLSSHIDLDRKANKNNPH			20	SLAY-screened peptide P3002			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGCTACCCTCTCCTCCCACATCGATCTCGATCGTAAGGCCAACAAGAATAATCCTCACTAA	SATLSSHIDLDRKANKNNPH*	-1.55	0.002177	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24653	HHYTTYLSYSI			11	SLAY-screened peptide P3003			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCACTATACCACGTATCTGTCGTATAGCATTTAGGAGCCGTCTACCTGCTATCTCAGGTAA	HHYTTYLSYSI*EPSTCYLR*	-1.549	0.047541	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24654	LSREEYWLSTPLCSHILMYA			20	SLAY-screened peptide P3004			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTCTCGTGAGGAGTATTGGCTTAGCACCCCGCTGTGCTCTCACATTCTGATGTACGCCTAA	LSREEYWLSTPLCSHILMYA*	-1.549	0.004782	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24655	RTDHLNSNCQALGASGTFTA			20	SLAY-screened peptide P3005			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACTGATCATCTGAACTCTAACTGCCAGGCGTTGGGGGCGTCGGGCACTTTTACTGCTTAA	RTDHLNSNCQALGASGTFTA*	-1.549	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24656	SIGYIIYHQMPPLMLSLLRW			20	SLAY-screened peptide P3006			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGATCGGTTATATCATTTATCACCAGATGCCTCCTCTTATGCTTAGCCTTCTCCGCTGGTAA	SIGYIIYHQMPPLMLSLLRW*	-1.549	0.033339	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24657	THTWSTPRCSSASRLFKINM			20	SLAY-screened peptide P3007			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCACACTTGGAGTACCCCTCGTTGTTCGTCTGCCAGCAGGCTCTTCAAGATTAACATGTAA	THTWSTPRCSSASRLFKINM*	-1.549	0.010901	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24658	TTTVSQEQYASRRSLKRTML			20	SLAY-screened peptide P3008			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACCACTGTCTCCCAGGAGCAGTACGCCAGTAGGCGGTCTCTGAAGCGTACCATGCTCTAA	TTTVSQEQYASRRSLKRTML*	-1.549	1.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24659	RCAPPINFPTSPPYLFYISH			20	SLAY-screened peptide P3009			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTGCGCTCCGCCCATCAACTTTCCGACCTCGCCGCCGTATCTGTTCTATATCAGTCATTAA	RCAPPINFPTSPPYLFYISH*	-1.549	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24660	RAGLCNICDCPHQAIDLVAL			20	SLAY-screened peptide P3010			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCCGGCCTTTGTAACATTTGCGACTGTCCCCACCAGGCTATCGATCTTGTTGCGCTTTAA	RAGLCNICDCPHQAIDLVAL*	-1.549	0.003046	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24661	YPCQFCYPRTNGTPFCLIPS			20	SLAY-screened peptide P3011			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCCCTGTCAGTTTTGCTATCCTCGCACGAACGGTACTCCGTTCTGCTTGATTCCTAGTTAA	YPCQFCYPRTNGTPFCLIPS*	-1.549	0.013461	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24662	TCRSP			5	SLAY-screened peptide P3012			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTGCCGCAGTCCTTGAACGACTTTCGCATGAATGATCTCCCCCCTTTGCTTCTGTATTAAC	TCRSP*TTFA*MISPLCFCIN	-1.549	0.015053	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24663	SQYRRIPGSNFSFSHLHFAL			20	SLAY-screened peptide P3013			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCAGTACCGTCGCATCCCTGGCTCCAATTTTAGTTTCTCCCATCTGCATTTTGCTCTGTAA	SQYRRIPGSNFSFSHLHFAL*	-1.548	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24664	TFRRFLMLGCTIFTACRKRL			20	SLAY-screened peptide P3014			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTTTCGGCGCTTTCTGATGCTGGGGTGCACGATTTTCACCGCTTGCCGGAAGCGCCTGTAA	TFRRFLMLGCTIFTACRKRL*	-1.548	6.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24665	SFRKNERRVRCTVPAMPCVF			20	SLAY-screened peptide P3015			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTTCCGCAAGAACGAGCGCCGCGTGAGGTGCACCGTGCCCGCCATGCCGTGCGTTTTTTAA	SFRKNERRVRCTVPAMPCVF*	-1.548	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24666	SCYAATVLTYPKPCLQSSAA			20	SLAY-screened peptide P3016			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTGTTATGCCGCGACGGTGCTTACCTATCCCAAGCCGTGCTTGCAGAGCTCCGCCGCTTAA	SCYAATVLTYPKPCLQSSAA*	-1.548	0.000183	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24667	PLG			3	SLAY-screened peptide P3017			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTGGGCTAGATTGTTTATACGTGCAAGGGCGGTATTTCTATCAGTGACTATACGACCTAA	PLG*IVYTCKGGISISDYTT*	-1.548	0.002142	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24668	NWSGSSIRTAWNLTGLCWHQ			20	SLAY-screened peptide P3018			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTGGTCGGGCTCCTCCATTCGTACGGCCTGGAACCTGACCGGGCTCTGCTGGCATCAGTAA	NWSGSSIRTAWNLTGLCWHQ*	-1.548	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24669	LFNYLAGVDLIRHFVDYTTC			20	SLAY-screened peptide P3019			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTTCAATTACCTGGCGGGGGTCGATTTGATTCGTCATTTTGTGGATTATACGACGTGTTAA	LFNYLAGVDLIRHFVDYTTC*	-1.548	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24670	PYASGHSCAWYTPIFIESMI			20	SLAY-screened peptide P3020			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTACGCCAGTGGCCATTCCTGTGCTTGGTACACCCCTATTTTCATCGAGTCTATGATTTAA	PYASGHSCAWYTPIFIESMI*	-1.547	0.039163	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24671	SSLIAVSSSTSIRLLRDYSQ			20	SLAY-screened peptide P3021			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTCGCTTATTGCCGTTAGTTCGTCCACGTCTATCCGTCTGCTTCGCGATTACTCTCAGTAA	SSLIAVSSSTSIRLLRDYSQ*	-1.547	0.000425	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24672	DHHCTIFISLADWNCLYTLA			20	SLAY-screened peptide P3022			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCATCATTGCACCATCTTTATCTCCCTTGCGGATTGGAACTGTCTTTACACCCTGGCCTAA	DHHCTIFISLADWNCLYTLA*	-1.547	0.00305	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24673	CPTSSFDSHNTKFQSDFAIV			20	SLAY-screened peptide P3023			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCCACCTCTAGCTTCGACAGCCATAATACTAAGTTTCAGTCTGATTTTGCTATTGTGTAA	CPTSSFDSHNTKFQSDFAIV*	-1.547	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24674	DSADQVRRFTVHYNQDEISD			20	SLAY-screened peptide P3024			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATAGCGCTGACCAGGTTAGGAGGTTTACCGTCCACTACAATCAGGATGAGATTTCGGATTAA	DSADQVRRFTVHYNQDEISD*	-1.546	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24675	PSPAFPSSINHVQAHCGYDR			20	SLAY-screened peptide P3025			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGCCCGGCCTTCCCTAGCTCCATCAATCATGTTCAGGCCCACTGCGGCTATGATCGGTAA	PSPAFPSSINHVQAHCGYDR*	-1.546	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24676	MTHAMKAPCLSFHDSDSSNR			20	SLAY-screened peptide P3026			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGACCCACGCGATGAAGGCGCCGTGCCTTTCTTTCCATGATAGTGATTCTAGCAATCGCTAA	MTHAMKAPCLSFHDSDSSNR*	-1.546	4.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24677	YKHPHANNCKYYIQITNAND			20	SLAY-screened peptide P3027			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAAGCATCCGCATGCTAATAACTGTAAGTACTATATTCAGATCACTAACGCTAATGACTAA	YKHPHANNCKYYIQITNAND*	-1.544	0.018362	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24678	SAYRPVCLVSTSPMYREIVFN			21	SLAY-screened peptide P3028			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCGTATCGTCCTGTGTGCCTGGTGAGTACGAGCCCCATGTACAGGGAAATCGTTTTTAAC	SAYRPVCLVSTSPMYREIVFN	-1.544	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24679	PLYNDRLHSLASTVQLLISA			20	SLAY-screened peptide P3029			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTCTATAACGATCGCCTTCACTCGCTGGCTTCTACGGTCCAGCTCTTGATCTCTGCCTAA	PLYNDRLHSLASTVQLLISA*	-1.544	0.000196	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24680	PSVMNPENGMTVSQLYCCIT			20	SLAY-screened peptide P3030			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGCGTTATGAACCCCGAGAACGGTATGACTGTCTCGCAGCTGTACTGTTGCATCACCTAA	PSVMNPENGMTVSQLYCCIT*	-1.544	0.000486	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24681	SLLGSPSIIIAVIAAVPDSLT			21	SLAY-screened peptide P3031			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCTCTTGGGTAGTCCTAGTATAATAATTGCGGTCATAGCCGCTGTGCCAGACTCCCTAACT	SLLGSPSIIIAVIAAVPDSLT	-1.544	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24682	LIRANILLHDGLPSAVRHPD			20	SLAY-screened peptide P3032			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGATTCGTGCGAACATCTTGCTTCACGACGGCCTCCCTTCGGCGGTCCGGCACCCTGACTAA	LIRANILLHDGLPSAVRHPD*	-1.544	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24683	CPWSYSLRRLRERIFFHLK			19	SLAY-screened peptide P3033			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCCTGGAGTTATTCGCTTCGGCGTCTTAGGGAGAGGATCTTTTTTCATCTTAAGTAGTAA	CPWSYSLRRLRERIFFHLK**	-1.543	0.022176	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24684	CTYSGDRPLATPTTRATCS			19	SLAY-screened peptide P3034			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTACTTATTCTGGTGACCGTCCTTTGGCTACGCCGACCACGCGGGCGACTTGCTCCTAGTAA	CTYSGDRPLATPTTRATCS**	-1.543	0.035104	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24685	SHKCDWHPNPSYAPVLSTSF			20	SLAY-screened peptide P3035			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCATAAGTGTGACTGGCACCCGAACCCTTCTTATGCTCCCGTCCTGAGTACGAGTTTCTAA	SHKCDWHPNPSYAPVLSTSF*	-1.543	0.003351	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24686	NYGGNILDSSYYTTATTLYT			20	SLAY-screened peptide P3036			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTATGGGGGTAATATCCTGGATTCTTCGTACTACACGACGGCTACCACTCTGTATACTTAA	NYGGNILDSSYYTTATTLYT*	-1.543	0.037286	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24687	TYFSRTDDLDRNHLHMCMSW			20	SLAY-screened peptide P3037			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTACTTCAGTCGGACTGACGACCTCGATCGCAATCACTTGCACATGTGTATGTCTTGGTAA	TYFSRTDDLDRNHLHMCMSW*	-1.543	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24688	VLDTLRAALTILLSYRRNSL			20	SLAY-screened peptide P3038			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCTCGACACGCTTCGTGCTGCTCTGACCATTCTTCTTTCTTACCGTCGTAACTCCTTGTAA	VLDTLRAALTILLSYRRNSL*	-1.542	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24689	TPKVQGGQDVAPPRPSPLFY			20	SLAY-screened peptide P3039			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCCCAAGGTCCAGGGCGGTCAGGACGTGGCTCCCCCTCGCCCTAGCCCCCTTTTCTACTAA	TPKVQGGQDVAPPRPSPLFY*	-1.542	0.012848	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24690	PLHMPIILSVIPNDQFTSAR			20	SLAY-screened peptide P3040			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCCATATGCCTATCATTCTCAGTGTTATTCCTAATGATCAGTTTACCTCTGCCCGTTAA	PLHMPIILSVIPNDQFTSAR*	-1.542	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24691	VSHSLCALLTTPSPRTPLCG			20	SLAY-screened peptide P3041			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTCCCACTCCCTCTGCGCTCTGCTCACGACCCCTTCGCCGAGGACCCCGCTTTGCGGCTAA	VSHSLCALLTTPSPRTPLCG*	-1.542	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24692	HPSIYSTSMYSLHCAWPSHT			20	SLAY-screened peptide P3042			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCGAGCATTTACTCCACCTCGATGTACTCCCTGCATTGCGCCTGGCCTTCTCACACCTAA	HPSIYSTSMYSLHCAWPSHT*	-1.541	0.028119	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24693	PPYYPCLIPTFSNPPYFNFR			20	SLAY-screened peptide P3043			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCGTACTATCCGTGCCTGATCCCGACGTTCAGTAATCCTCCTTATTTTAACTTCCGTTAA	PPYYPCLIPTFSNPPYFNFR*	-1.541	0.004342	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24694	GPSARPTSNLFCMHRSGHAT			20	SLAY-screened peptide P3044			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCCTTCGGCCAGGCCGACTAGTAATCTCTTTTGTATGCACCGTTCCGGCCATGCTACCTAA	GPSARPTSNLFCMHRSGHAT*	-1.541	0.000344	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24695	QPQKSRFSTNLDQKYNHRPS			20	SLAY-screened peptide P3045			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCCCAGAAGTCTCGGTTCTCGACCAACCTGGACCAGAAGTACAACCACCGGCCCTCGTAA	QPQKSRFSTNLDQKYNHRPS*	-1.541	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24696	PSPYTNVVKNSTRAGYGPYK			20	SLAY-screened peptide P3046			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTCGCCCTACACCAACGTTGTGAAGAACTCTACCCGCGCCGGGTATGGCCCTTACAAGTAA	PSPYTNVVKNSTRAGYGPYK*	-1.541	0.007947	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24697	PSKCDQSS			8	SLAY-screened peptide P3047			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGTAAGTGCGATCAGAGTAGTTAGTTGCCGTATAATTGTTCGACCTATGACTAGTGCTAA	PSKCDQSS*LPYNCSTYD*C*	-1.541	0.000224	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24698	YRFQAATGLLADRFPRHAVD			20	SLAY-screened peptide P3048			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCGGTTTCAGGCTGCCACTGGGCTCCTCGCCGACCGTTTTCCCAGGCATGCCGTCGACTAA	YRFQAATGLLADRFPRHAVD*	-1.541	0.046101	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24699	QVFHPS			6	SLAY-screened peptide P3049			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGTGTTTCACCCGTCCTAGGACAAGGCTCCCATGGAGACTGGTGTTACCTACGTCTTCTAA	QVFHPS*DKAPMETGVTYVF*	-1.54	0.047036	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24700	TLTICPVRSGTWHIALFGIP			20	SLAY-screened peptide P3050			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTTGACGATTTGCCCTGTGAGGAGTGGCACTTGGCACATCGCGCTTTTTGGCATTCCTTAA	TLTICPVRSGTWHIALFGIP*	-1.539	0.000554	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24701	TYNCSTLRSIRMTLI			15	SLAY-screened peptide P3051			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTACAATTGTTCTACTCTGCGCTCCATCCGCATGACGCTTATTTAGAGTGCCACCAATTAA	TYNCSTLRSIRMTLI*SATN*	-1.539	0.002448	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24702	RFAVPSPCPYYCLSYPYVYL			20	SLAY-screened peptide P3052			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTTCGCTGTGCCCTCCCCCTGTCCCTACTACTGCCTGAGCTACCCCTACGTTTACCTGTAA	RFAVPSPCPYYCLSYPYVYL*	-1.538	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24703	SINGKRWPGA			10	SLAY-screened peptide P3053			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTATTAATGGTAAGCGTTGGCCCGGTGCCTAGCTCTGTAAGTATCGTTGCAACACTACGTAA	SINGKRWPGA*LCKYRCNTT*	-1.538	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24704	RHNEMNSGDSIRCLPTMS			18	SLAY-screened peptide P3054			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCACAACGAGATGAACTCGGGTGACTCCATCCGTTGTCTTCCCACTATGTCGTAGCATTAA	RHNEMNSGDSIRCLPTMS*H*	-1.538	0.001192	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24705	DWKLPTRTLRLFRRICPTRVN			21	SLAY-screened peptide P3055			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTGGAAGCTCCCCACCAGAACATTACGATTATTCAGGCGTATCTGCCCGACACGCGTTAAC	DWKLPTRTLRLFRRICPTRVN	-1.538	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24706	FYPFHGSHFPVPYINNCDPA			20	SLAY-screened peptide P3056			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTATCCTTTCCACGGTAGCCACTTCCCTGTGCCGTATATTAACAATTGCGATCCCGCGTAA	FYPFHGSHFPVPYINNCDPA*	-1.538	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24707	PSCDLWDCRFRCLTLWPTVH			20	SLAY-screened peptide P3057			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAGCTGTGATTTGTGGGATTGCCGGTTTAGGTGTCTCACCCTCTGGCCGACCGTCCATTAA	PSCDLWDCRFRCLTLWPTVH*	-1.538	0.000333	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24708	ASRETDPSTSNCTDSCAFKS			20	SLAY-screened peptide P3058			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTCTCGCGAGACTGACCCCTCCACTTCCAACTGCACGGACTCCTGCGCCTTTAAGAGCTAA	ASRETDPSTSNCTDSCAFKS*	-1.537	0.020004	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24709	SSTCYVLHPSMRNTHRDSPG			20	SLAY-screened peptide P3059			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTCGACGTGCTATGTCTTGCACCCCAGTATGCGCAATACTCATCGCGATTCGCCCGGTTAA	SSTCYVLHPSMRNTHRDSPG*	-1.537	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24710	LLHVRLGLSFLPLRCWAPQV			20	SLAY-screened peptide P3060			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTTGCATGTCAGGCTCGGCCTCAGCTTTCTGCCCCTCCGTTGTTGGGCGCCTCAGGTTTAA	LLHVRLGLSFLPLRCWAPQV*	-1.537	0.011351	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24711	HDAILAVRKHGNRLQICGAN			20	SLAY-screened peptide P3061			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGACGCTATTCTGGCGGTTAGGAAGCATGGTAATAGGCTTCAGATTTGTGGGGCTAACTAA	HDAILAVRKHGNRLQICGAN*	-1.537	0.001766	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24712	YHSLRTSRRPSPTDVSITYS			20	SLAY-screened peptide P3062			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCACTCCCTTCGGACGAGTCGCCGCCCGTCTCCGACTGATGTGTCCATCACCTATAGTTAA	YHSLRTSRRPSPTDVSITYS*	-1.537	0.003343	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24713	MATPASQVVVLQGLPTVNPN			20	SLAY-screened peptide P3063			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGCCACTCCTGCTAGCCAGGTCGTTGTTTTGCAGGGCCTGCCGACTGTTAATCCCAACTAA	MATPASQVVVLQGLPTVNPN*	-1.537	0.000577	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24714	AYDPDSQNSNYCMNHTFYKD			20	SLAY-screened peptide P3064			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTACGATCCCGACTCCCAGAATTCGAATTATTGCATGAATCATACCTTCTATAAGGATTAA	AYDPDSQNSNYCMNHTFYKD*	-1.536	0.00558	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24715	LRLRIRRLTVSVIVVSFMLSN			21	SLAY-screened peptide P3065			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAGGTTGAGAATTCGTCGCCTCACCGTTAGTGTTATTGTAGTAAGCTTTATGCTCAGTAAC	LRLRIRRLTVSVIVVSFMLSN	-1.536	0.000223	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24716	PAPLYTRALSAFLM			14	SLAY-screened peptide P3066			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCCCCTCTTTATACTAGGGCCTTATCGGCGTTCCTGATGTGAGTCAGCCGGACCGTTACT	PAPLYTRALSAFLM*VSRTVT	-1.536	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24717	FSGSHFPFYPLGHPCYVLRY			20	SLAY-screened peptide P3067			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTCTGGGTCTCATTTTCCTTTCTACCCCCTCGGTCACCCCTGCTATGTCCTCAGGTATTAA	FSGSHFPFYPLGHPCYVLRY*	-1.536	3.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24718	FHCSCSARSLTFWYHDSLNH			20	SLAY-screened peptide P3068			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCACTGTTCCTGCTCTGCCAGGTCCTTGACCTTCTGGTATCACGATTCGCTCAATCACTAA	FHCSCSARSLTFWYHDSLNH*	-1.536	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24719	MRCTAQDNIAAKLPKNGLAY			20	SLAY-screened peptide P3069			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCGGTGTACGGCCCAGGATAATATTGCGGCCAAGCTTCCCAAGAATGGGTTGGCTTATTAA	MRCTAQDNIAAKLPKNGLAY*	-1.535	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24720	NTPETSPGLRHCNCRTAVNF			20	SLAY-screened peptide P3070			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACACCCCCGAGACGAGCCCTGGTTTGCGCCATTGTAATTGTAGGACGGCTGTGAACTTTTAA	NTPETSPGLRHCNCRTAVNF*	-1.535	7.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24721	RSYTRSRFPHFLNNWIRVAR			20	SLAY-screened peptide P3071			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAGCTATACTAGGTCCCGGTTTCCCCATTTTCTCAACAACTGGATTCGCGTTGCCCGGTAA	RSYTRSRFPHFLNNWIRVAR*	-1.535	0.003358	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24722	PYPCLPGFSNIVSNIPDCKL			20	SLAY-screened peptide P3072			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTACCCCTGCTTGCCCGGTTTCAGCAATATTGTTTCTAATATCCCTGATTGCAAGCTGTAA	PYPCLPGFSNIVSNIPDCKL*	-1.535	0.042403	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24723	TTTSLLSRLPPNYMAYSSAH			20	SLAY-screened peptide P3073			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACGACGAGTCTTCTTTCTCGGCTTCCGCCCAATTATATGGCCTATTCGTCGGCCCATTAA	TTTSLLSRLPPNYMAYSSAH*	-1.535	0.000418	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24724	RIHGHDLRVHRPLRASTRQS			20	SLAY-screened peptide P3074			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATCCACGGTCATGATCTCCGTGTCCACCGTCCCTTGCGGGCGTCGACGCGCCAGTCGTAA	RIHGHDLRVHRPLRASTRQS*	-1.535	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24725	IPYGATQLNKSQHRFNSFYR			20	SLAY-screened peptide P3075			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCCTTACGGGGCCACCCAGTTGAATAAGTCCCAGCACCGGTTCAATAGTTTTTACAGGTAA	IPYGATQLNKSQHRFNSFYR*	-1.535	0.018909	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24726	IDCLRAFISILFKIIFLYTV			20	SLAY-screened peptide P3076			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGACTGCCTCCGTGCTTTTATCTCCATTCTGTTCAAGATCATTTTCTTGTACACCGTCTAA	IDCLRAFISILFKIIFLYTV*	-1.535	0.004502	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24727	SRAQRRSICSCLMMSPQPLA			20	SLAY-screened peptide P3077			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCGCGCTCAGCGCAGGTCGATCTGCAGCTGCTTGATGATGTCGCCTCAGCCCCTCGCTTAA	SRAQRRSICSCLMMSPQPLA*	-1.534	0.017212	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24728	WWTLYNYSTTPYAVHGGSFLN			21	SLAY-screened peptide P3078			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTGGACTTTGTACAATTACAGTACCACTCCCTATGCCGTCCATGGTGGATCGTTTCTTAAC	WWTLYNYSTTPYAVHGGSFLN	-1.534	0.000182	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24729	IIHHVTTGHNS			11	SLAY-screened peptide P3079			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCATCCATCACGTCACCACGGGGCACAACTCTTAGCTTAAGACTGCGTCTGCCTACGCTTAA	IIHHVTTGHNS*LKTASAYA*	-1.534	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24730	RDELLHLSYSVPSTHHSRRF			20	SLAY-screened peptide P3080			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGACGAGCTCCTGCATCTTTCTTATTCTGTCCCCAGTACCCACCATTCGCGCCGTTTTTAA	RDELLHLSYSVPSTHHSRRF*	-1.534	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24731	HHCRIGTMSTHHTPRYIECS			20	SLAY-screened peptide P3081			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCACTGTCGTATCGGTACTATGTCTACCCATCATACTCCGAGGTACATCGAGTGCAGTTAA	HHCRIGTMSTHHTPRYIECS*	-1.534	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24732	TLTQGYHSLPVFTISHTTPL			20	SLAY-screened peptide P3082			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTCACGCAGGGTTACCACAGTCTGCCCGTCTTTACGATTTCCCATACGACGCCCCTGTAA	TLTQGYHSLPVFTISHTTPL*	-1.534	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24733	PSRTMDTLNSDRH			13	SLAY-screened peptide P3083			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGTCGCACCATGGATACTCTTAATTCGGATCGGCACTAGCTTATGGCGGGCCATCCCTAA	PSRTMDTLNSDRH*LMAGHP*	-1.533	0.003336	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24734	PYSPCDYTTTLSRVNSSFIR			20	SLAY-screened peptide P3084			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTATTCCCCGTGTGACTATACGACGACCCTTAGTCGTGTGAATTCCAGCTTCATCCGCTAA	PYSPCDYTTTLSRVNSSFIR*	-1.533	0.018555	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24735	HNACSLAHPIYPLQIRNTCN			20	SLAY-screened peptide P3085			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAATGCGTGCTCCTTGGCCCACCCTATCTACCCTCTTCAGATTCGGAACACCTGCAATTAA	HNACSLAHPIYPLQIRNTCN*	-1.533	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24736	RRLLLPKPRFCTPAFCPRLSN			21	SLAY-screened peptide P3086			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGGCTCCTTCTGCCCAAACCTCGCTTCTGTACACCAGCCTTTTGCCCGCGACTCAGTAAC	RRLLLPKPRFCTPAFCPRLSN	-1.533	0.008045	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24737	TTLTRQVGRISDGGGILPAE			20	SLAY-screened peptide P3087			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACCCTCACTCGTCAGGTGGGGCGCATTAGCGATGGCGGCGGTATTTTGCCTGCCGAGTAA	TTLTRQVGRISDGGGILPAE*	-1.533	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24738	FKRPYSNSRKSNRTTSTLSM			20	SLAY-screened peptide P3088			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTAAGCGGCCCTACTCTAACAGTCGCAAGAGCAATCGTACCACTAGTACGCTTAGCATGTAA	FKRPYSNSRKSNRTTSTLSM*	-1.532	0.010611	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24739	YSRLFGSCRFSNRYYNHRDF			20	SLAY-screened peptide P3089			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTCTCGTCTTTTCGGCAGTTGCCGTTTCTCTAACCGTTACTACAATCATCGGGACTTCTAA	YSRLFGSCRFSNRYYNHRDF*	-1.532	0.032006	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24740	YLIN			4	SLAY-screened peptide P3090			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTTGATCAACTAGGAGACCGGTCTGTGCGGGACTCATGTCACCTGCACGGTTGACCTGTAA	YLIN*ETGLCGTHVTCTVDL*	-1.532	0.002373	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24741	QPTFTVYDVHYNHNYGASKR			20	SLAY-screened peptide P3091			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCGACTTTTACGGTCTATGATGTCCATTATAACCATAATTACGGGGCGTCCAAGCGGTAA	QPTFTVYDVHYNHNYGASKR*	-1.532	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24742	FPGDAIHIHFRAWRRTKRHH			20	SLAY-screened peptide P3092			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCCTGGGGACGCCATTCATATCCACTTTCGCGCGTGGCGTAGGACGAAGCGGCATCATTAA	FPGDAIHIHFRAWRRTKRHH*	-1.532	0.011094	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24743	PRLQHSYTFVACDYDTHANL			20	SLAY-screened peptide P3093			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGTCTCCAGCATAGCTACACGTTTGTTGCTTGTGACTATGATACGCATGCTAACCTCTAA	PRLQHSYTFVACDYDTHANL*	-1.531	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24744	PYNALPSIQTAVKEESLCAS			20	SLAY-screened peptide P3094			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTACAACGCTTTGCCTTCCATTCAGACGGCCGTGAAGGAGGAGAGCCTTTGCGCCAGCTAA	PYNALPSIQTAVKEESLCAS*	-1.531	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24745	HRVTSSALYDSNVISMEQFP			20	SLAY-screened peptide P3095			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCGCGTTACTAGTAGCGCCCTTTACGATTCTAATGTGATCAGCATGGAGCAGTTTCCCTAA	HRVTSSALYDSNVISMEQFP*	-1.531	0.002627	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24746	TDHPFDYNHGVSQANRNNYY			20	SLAY-screened peptide P3096			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGACCACCCCTTCGATTATAACCACGGTGTTTCCCAGGCTAACCGTAACAACTATTATTAA	TDHPFDYNHGVSQANRNNYY*	-1.531	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24747	RPCPRSSSPHTDKRCRCMLR			20	SLAY-screened peptide P3097			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCCTGCCCCAGGTCCTCGAGTCCTCATACTGACAAGCGCTGTCGCTGCATGCTTCGGTAA	RPCPRSSSPHTDKRCRCMLR*	-1.531	0.000676	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24748	VPPALQTGNNICLSNYFTRL			20	SLAY-screened peptide P3098			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCCCCCTGCTCTCCAGACCGGTAACAACATCTGCCTCTCCAATTACTTCACGAGGCTGTAA	VPPALQTGNNICLSNYFTRL*	-1.531	2.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24749	STNPTNVVTHDNIYDDVDNG			20	SLAY-screened peptide P3099			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTACCAATCCGACGAACGTCGTTACGCACGATAACATCTATGACGATGTTGACAACGGGTAA	STNPTNVVTHDNIYDDVDNG*	-1.53	0.006087	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24750	PLAGYSAFNHLYVNSSRVRK			20	SLAY-screened peptide P3100			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTGGCGGGCTACTCTGCTTTTAATCACCTCTATGTTAATTCCAGTCGCGTGCGTAAGTAA	PLAGYSAFNHLYVNSSRVRK*	-1.53	0.006088	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24751	KKSVSSHCTPLGTDRIDSHT			20	SLAY-screened peptide P3101			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGAAGTCCGTTTCTAGCCACTGTACTCCCCTCGGTACTGATAGGATCGACTCCCATACGTAA	KKSVSSHCTPLGTDRIDSHT*	-1.53	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24752	VPGCRAGSPSSLPSEPSLFM			20	SLAY-screened peptide P3102			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCCTGGGTGCCGTGCCGGTTCGCCGAGCAGTCTCCCGTCCGAGCCTAGCCTGTTTATGTAA	VPGCRAGSPSSLPSEPSLFM*	-1.53	0.009701	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24753	PIALPAGDRFYMPLSFPSSY			20	SLAY-screened peptide P3103			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATTGCTCTCCCTGCCGGTGATCGCTTCTATATGCCGCTCTCCTTCCCCTCGTCTTACTAA	PIALPAGDRFYMPLSFPSSY*	-1.53	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24754	TPPWSNSRTIDNPSVLHEDA			20	SLAY-screened peptide P3104			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCCCCGTGGTCTAACAGCCGTACGATTGACAATCCCTCCGTTCTTCACGAGGATGCCTAA	TPPWSNSRTIDNPSVLHEDA*	-1.53	0.034592	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24755	HTLYSPTYHKRVIAHNLIRS			20	SLAY-screened peptide P3105			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACGTTGTACTCGCCCACGTATCACAAGCGCGTGATTGCTCACAATCTGATTCGGAGCTAA	HTLYSPTYHKRVIAHNLIRS*	-1.53	0.000761	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24756	YPTTLYLIFNCSANNRCLLS			20	SLAY-screened peptide P3106			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCCCACTACTCTTTACCTTATCTTCAACTGTAGTGCCAACAATAGGTGCTTGTTGAGTTAA	YPTTLYLIFNCSANNRCLLS*	-1.529	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24757	HPRTSPHRPCSFDWKYLARL			20	SLAY-screened peptide P3107			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCGCGCACGTCGCCTCATCGGCCCTGTAGCTTTGACTGGAAGTACTTGGCTAGGCTTTAA	HPRTSPHRPCSFDWKYLARL*	-1.529	2.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24758	AQRAVVISVFCTVWFDLSTG			20	SLAY-screened peptide P3108			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCAGAGGGCTGTGGTTATCAGTGTTTTCTGCACTGTTTGGTTCGACCTTTCTACGGGCTAA	AQRAVVISVFCTVWFDLSTG*	-1.529	0.023708	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24759	DLDMDGYLFYHMLPLNMC			18	SLAY-screened peptide P3109			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCTGGATATGGATGGCTATTTGTTTTATCACATGCTTCCGCTTAACATGTGCTAACTGAGT	DLDMDGYLFYHMLPLNMC*LS	-1.529	0.000101	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24760	SAM			3	SLAY-screened peptide P3110			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGCCATGTAGGACCCTTATAGCCCTGCGTAGGCGCCTTACATTGAGAATGGGACCAAGTAA	SAM*DPYSPA*APYIENGTK*	-1.529	2.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24761	SCNTTTFPRYPGYTYYLCNF			20	SLAY-screened peptide P3111			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTGTAACACTACCACGTTTCCCAGGTACCCTGGTTATACTTATTACCTTTGTAACTTTTAA	SCNTTTFPRYPGYTYYLCNF*	-1.529	0.045364	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24762	HLCFRSPTLGVIAYHNPCVR			20	SLAY-screened peptide P3112			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTTGTGCTTCCGGTCTCCTACTCTCGGTGTCATTGCGTACCACAATCCGTGCGTCCGTTAA	HLCFRSPTLGVIAYHNPCVR*	-1.529	0.006405	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24763	TCNSPSRYGNAWPSYTIIGIN			21	SLAY-screened peptide P3113			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTGCAACAGCCCTTCTAGGTACGGCAACGCCTGGCCTAGTTATACCATCATCGGTATTAAC	TCNSPSRYGNAWPSYTIIGIN	-1.528	0.024284	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24764	SARRRLTQFRTLIVSYHNGT			20	SLAY-screened peptide P3114			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGCCAGGAGGCGGCTCACGCAGTTCAGGACGCTTATCGTCAGTTACCATAACGGCACCTAA	SARRRLTQFRTLIVSYHNGT*	-1.528	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24765	LFMLIAIFPARGSSRRYQAY			20	SLAY-screened peptide P3115			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTTTATGCTCATCGCTATCTTCCCCGCCCGCGGTTCGAGTCGCAGGTACCAGGCGTATTAA	LFMLIAIFPARGSSRRYQAY*	-1.528	0.022385	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24766	SSLVWDFTNTAPNIVTAMPK			20	SLAY-screened peptide P3116			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCAGCCTCGTGTGGGATTTTACCAACACGGCGCCTAATATTGTGACCGCGATGCCGAAGTAA	SSLVWDFTNTAPNIVTAMPK*	-1.527	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24767	LRSVFVMFDKPWYDYFLIAQ			20	SLAY-screened peptide P3117			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCGCTCCGTCTTTGTTATGTTTGACAAGCCTTGGTATGATTATTTCCTGATCGCTCAGTAA	LRSVFVMFDKPWYDYFLIAQ*	-1.527	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24768	RTYY			4	SLAY-screened peptide P3118			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACTTATTATTAGGACCTTCCCACTTTGTCGCGTAAGCATTACATCCTTTGGAGTTCCTAA	RTYY*DLPTLSRKHYILWSS*	-1.527	0.042431	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24769	ELHHWNPAFVKLRSTWWGPIN			21	SLAY-screened peptide P3119			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGTTGCATCACTGGAATCCCGCGTTTGTGAAGTTGCGAAGCACATGGTGGGGACCAATTAAC	ELHHWNPAFVKLRSTWWGPIN	-1.526	0.009587	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24770	FGDPGHLRCVAITKACNPFI			20	SLAY-screened peptide P3120			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGGTGATCCGGGTCACCTCCGGTGCGTTGCCATTACCAAGGCTTGTAATCCCTTCATTTAA	FGDPGHLRCVAITKACNPFI*	-1.526	4.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24771	VHYVNYGFSYVWIIRCDLPS			20	SLAY-screened peptide P3121			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCACTACGTTAATTACGGTTTTTCCTATGTCTGGATCATTCGTTGTGACCTCCCTAGTTAA	VHYVNYGFSYVWIIRCDLPS*	-1.525	0.00011	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24772	SCPVLPLPLFGTLLNYVAEQ			20	SLAY-screened peptide P3122			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTGTCCTGTCCTTCCTCTTCCCCTTTTTGGCACTCTCTTGAACTACGTCGCGGAGCAGTAA	SCPVLPLPLFGTLLNYVAEQ*	-1.525	0.001619	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24773	NANMNVFRIGTSLYNLTISH			20	SLAY-screened peptide P3123			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGCGAATATGAACGTTTTCAGGATTGGTACCAGTTTGTATAATTTGACCATTAGTCATTAA	NANMNVFRIGTSLYNLTISH*	-1.525	0.001243	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24774	TCI			3	SLAY-screened peptide P3124			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTGCATTTAGTACTACCTGTCGACCTACCATATTCCGAGCGTCCACTGGATCTTGGCCTAA	TCI*YYLSTYHIPSVHWILA*	-1.525	5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24775	CLFL			4	SLAY-screened peptide P3125			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTCTTTCTTTAGGCCTTGTTTTCTGACAGCTGTATTGTGTGTTTCCATGATTTTAGGTAA	CLFL*ALFSDSCIVCFHDFR*	-1.525	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24776	PFNRAS			6	SLAY-screened peptide P3126			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTTAACCGCGCTTCTTAGACTAATTTTTAGGAGCGTCTCAACTTTATTCACGAGTGGTAA	PFNRAS*TNF*ERLNFIHEW*	-1.524	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24777	DFSVQSNELSITLCFLIFFLN			21	SLAY-screened peptide P3127			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTTCAGTGTTCAGAGCAACGAGTTGTCCATTACTCTGTGCTTTTTAATTTTTTTTCTTAAC	DFSVQSNELSITLCFLIFFLN	-1.524	0.001709	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24778	LYHCYLAR			8	SLAY-screened peptide P3128			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTATCACTGCTACCTCGCTCGGTAGGCCTTTGGTCATGGCAGTAACCATAACGGGAAGTAA	LYHCYLAR*AFGHGSNHNGK*	-1.524	0.037971	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24779	LTRRRPTFQSPRPANVTKTQ			20	SLAY-screened peptide P3129			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCACTAGGCGGCGGCCTACTTTTCAGAGTCCGCGCCCCGCTAATGTTACTAAGACGCAGTAA	LTRRRPTFQSPRPANVTKTQ*	-1.523	0.000276	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24780	DANTCIGDRYDSDADAISLV			20	SLAY-screened peptide P3130			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGCCAACACTTGCATCGGTGACCGCTATGACAGTGATGCGGACGCCATTAGTTTGGTCTAA	DANTCIGDRYDSDADAISLV*	-1.523	0.012066	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24781	RICLRRVVISTPSTCSICLFN			21	SLAY-screened peptide P3131			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATTTGCCTCCGCCGTGTGGTTATCAGCACTCCATCAACTTGTTCTATCTGCCTGTTTAAC	RICLRRVVISTPSTCSICLFN	-1.523	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24782	TDFNSHSKLYGCVMRNTNSS			20	SLAY-screened peptide P3132			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGACTTTAACAGCCACTCTAAGCTTTATGGGTGCGTTATGCGTAATACGAATTCTAGCTAA	TDFNSHSKLYGCVMRNTNSS*	-1.523	0.04764	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24783	SRNSGHPPAIFDHLILSYPA			20	SLAY-screened peptide P3133			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCGTAATTCCGGTCACCCGCCGGCTATCTTCGATCATCTTATTTTGTCCTATCCTGCCTAA	SRNSGHPPAIFDHLILSYPA*	-1.522	0.001304	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24784	FHVRSTQTNMGFANRYDRFQ			20	SLAY-screened peptide P3134			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCACGTTAGGTCGACTCAGACCAATATGGGTTTTGCGAATAGGTACGATAGGTTTCAGTAA	FHVRSTQTNMGFANRYDRFQ*	-1.522	0.03846	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24785	CPARPTTTNEEYMRYEYAPY			20	SLAY-screened peptide P3135			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCTGCTCGTCCTACTACCACCAACGAGGAGTATATGCGTTATGAGTACGCTCCTTATTAA	CPARPTTTNEEYMRYEYAPY*	-1.522	3.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24786	PDVKILPNPENLRFCRWYYI			20	SLAY-screened peptide P3136			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGACGTGAAGATTCTTCCCAACCCTGAGAACCTTAGGTTTTGCAGGTGGTATTACATTTAA	PDVKILPNPENLRFCRWYYI*	-1.522	0.002159	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24787	YTTLPNRHQYRISFCSTYYS			20	SLAY-screened peptide P3137			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACTACTCTCCCGAACCGCCATCAGTACAGGATTTCTTTTTGTTCCACTTATTACTCTTAA	YTTLPNRHQYRISFCSTYYS*	-1.521	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24788	PINTYTSVQSRCPDLNLRAG			20	SLAY-screened peptide P3138			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATCAACACTTACACCAGTGTCCAGAGTCGCTGTCCTGACCTTAACCTCAGGGCGGGCTAA	PINTYTSVQSRCPDLNLRAG*	-1.521	3.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24789	HNVTRFTMSWNFSGTN			16	SLAY-screened peptide P3139			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAATGTCACGCGTTTCACGATGAGTTGGAATTTCTCGGGCACCAATTAGCAGGATGTTTAA	HNVTRFTMSWNFSGTN*QDV*	-1.521	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24790	FDCWRVPTTGCYAPLFTIIL			20	SLAY-screened peptide P3140			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGATTGCTGGAGGGTTCCGACTACGGGCTGTTATGCCCCTCTTTTCACGATTATCCTGTAA	FDCWRVPTTGCYAPLFTIIL*	-1.521	0.001299	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24791	TIGPLRVYSLTRFDYRVILY			20	SLAY-screened peptide P3141			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCATTGGCCCGCTCCGTGTTTACTCGCTTACCCGGTTTGATTACAGGGTCATTTTGTATTAA	TIGPLRVYSLTRFDYRVILY*	-1.521	0.024896	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24792	VSMRTHPLSSASVLTHSHFT			20	SLAY-screened peptide P3142			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCAGTATGCGGACCCATCCCCTCTCCTCGGCGAGTGTTCTTACTCACTCTCACTTTACTTAA	VSMRTHPLSSASVLTHSHFT*	-1.52	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24793	PIAGLTTYRPILFKPDHNGK			20	SLAY-screened peptide P3143			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATCGCCGGTCTCACTACCTACCGTCCGATCTTGTTTAAGCCTGATCACAACGGGAAGTAA	PIAGLTTYRPILFKPDHNGK*	-1.52	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24794	NNPNVRARRLPLPSALVIRSN			21	SLAY-screened peptide P3144			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACAATCCTAACGTGCGTGCTCGACGCCTTCCCCTACCGTCCGCACTAGTGATTCGCTCTAAC	NNPNVRARRLPLPSALVIRSN	-1.52	0.001299	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24795	YGLVYHKMCVAPKVAIHAD			19	SLAY-screened peptide P3145			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGGCCTCGTCTACCATAAGATGTGTGTTGCCCCCAAGGTTGCTATCCACGCCGATTAGTAA	YGLVYHKMCVAPKVAIHAD**	-1.52	0.000624	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24796	RNCPTPPNRATSWSLPMLNG			20	SLAY-screened peptide P3146			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGAACTGCCCGACTCCCCCCAACCGTGCTACTTCGTGGTCGTTGCCGATGTTGAATGGTTAA	RNCPTPPNRATSWSLPMLNG*	-1.52	0.03504	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24797	TTSNGFYYDTHYTLLPGYYV			20	SLAY-screened peptide P3147			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGACCTCGAATGGCTTCTACTACGACACCCATTATACTCTCTTGCCCGGTTACTATGTTTAA	TTSNGFYYDTHYTLLPGYYV*	-1.52	0.000302	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24798	LPHAANPRPHIANYMNINC			19	SLAY-screened peptide P3148			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCTCATGCTGCCAACCCTCGCCCTCATATTGCTAATTATATGAACATCAATTGCTAGTAA	LPHAANPRPHIANYMNINC**	-1.519	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24799	HTLPHCPLMMMKIMYLNRNH			20	SLAY-screened peptide P3149			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACGCTTCCTCACTGCCCCCTGATGATGATGAAGATTATGTATCTCAACCGCAATCATTAA	HTLPHCPLMMMKIMYLNRNH*	-1.519	0.003326	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24800	SECTMYSKRFPTNIMLPSLI			20	SLAY-screened peptide P3150			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGAGTGCACCATGTATTCGAAGAGGTTTCCTACGAACATTATGCTTCCCTCCCTGATTTAA	SECTMYSKRFPTNIMLPSLI*	-1.519	0.000582	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24801	VFLAITSN			8	SLAY-screened peptide P3151			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTTTCTCGCTATCACCTCGAACTAGCACGTGCCCCACATTACCAATCTTGTTATGTCGTAA	VFLAITSN*HVPHITNLVMS*	-1.519	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24802	PPFYLFLSLDTTTTPRPPTG			20	SLAY-screened peptide P3152			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTTTCTATCTTTTCTTGAGCCTCGACACCACTACGACGCCTCGTCCTCCGACGGGCTAA	PPFYLFLSLDTTTTPRPPTG*	-1.519	0.00121	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24803	LYFTTNPDQFQNSKKWIPRA			20	SLAY-screened peptide P3153			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTACTTTACTACGAACCCTGATCAGTTCCAGAACTCCAAGAAGTGGATTCCTCGGGCCTAA	LYFTTNPDQFQNSKKWIPRA*	-1.519	0.000646	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24804	CGPSYRTLPLFVAWCCS			17	SLAY-screened peptide P3154			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGGTCCCAGCTACCGGACCCTGCCGCTCTTCGTTGCCTGGTGTTGCAGTTAGAGTAGCTAA	CGPSYRTLPLFVAWCCS*SS*	-1.519	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24805	RNLYAVKRNTGPFHLSTHPH			20	SLAY-screened peptide P3155			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAATCTCTACGCCGTGAAGCGGAATACTGGTCCCTTTCACCTGAGCACCCACCCGCACTAA	RNLYAVKRNTGPFHLSTHPH*	-1.519	0.000401	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24806	EIVLCTDIRDQSHPFQPGST			20	SLAY-screened peptide P3156			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGATTGTTCTTTGCACTGACATCAGGGACCAGAGTCATCCTTTCCAGCCCGGCTCGACTTAA	EIVLCTDIRDQSHPFQPGST*	-1.518	0.000159	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24807	KQGGRLFHFLIYLKRRFCAR			20	SLAY-screened peptide P3157			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCAGGGGGGCCGCCTTTTCCACTTCCTGATCTATCTCAAGCGTCGTTTCTGTGCCCGTTAA	KQGGRLFHFLIYLKRRFCAR*	-1.518	6.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24808	WHDSLRCHSPLRVAKHWPRL			20	SLAY-screened peptide P3158			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCATGATTCCCTGCGGTGTCACTCGCCTCTTCGTGTCGCCAAGCATTGGCCGCGGCTTTAA	WHDSLRCHSPLRVAKHWPRL*	-1.518	0.00017	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24809	IYDCN			5	SLAY-screened peptide P3159			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTTACGATTGTAATTAGGGCAGTTATAATGCGAATCGCCAGGGTTCGTGTGTGTGGCATTAA	IYDCN*GSYNANRQGSCVWH*	-1.518	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24810	IYIRVFCLLIIRIR			14	SLAY-screened peptide P3160			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTACATTAGGGTCTTTTGTCTTCTGATCATTCGTATTAGGTAGTATACGTTTGGTAGCTAA	IYIRVFCLLIIRIR*YTFGS*	-1.518	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24811	THVFNLPDNLLYHCNSHFDS			20	SLAY-screened peptide P3161			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCACGTCTTCAATTTGCCTGACAATCTCCTCTATCATTGCAATTCTCACTTCGATTCGTAA	THVFNLPDNLLYHCNSHFDS*	-1.517	0.005088	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24812	HDLYNVKKRD			10	SLAY-screened peptide P3162			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGATCTTTACAACGTCAAGAAGAGGGACTAGGTTAGCTTCCGGCTTACCTTTTTCAGCTAA	HDLYNVKKRD*VSFRLTFFS*	-1.517	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24813	NSVIRPRHN			9	SLAY-screened peptide P3163			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTCCGTGATCCGCCCGCGCCACAATTAGTCGGTCGGTAGGGATCTTGATGCTACTTAGTAA	NSVIRPRHN*SVGRDLDAT**	-1.516	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24814	PSRSPTSPDPFLKAILTNLV			20	SLAY-screened peptide P3164			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCCCGGTCGCCCACCAGTCCGGATCCCTTTCTCAAGGCTATCTTGACGAATCTCGTTTAA	PSRSPTSPDPFLKAILTNLV*	-1.516	4.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24815	PALLTSPHSDLCHSVYYDLA			20	SLAY-screened peptide P3165			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCCTTGCTTACCAGCCCGCATAGCGACCTCTGCCACAGTGTCTATTACGATCTGGCTTAA	PALLTSPHSDLCHSVYYDLA*	-1.516	0.005969	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24816	LVLNRWSHPHSNPMRSYSRQ			20	SLAY-screened peptide P3166			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGTTCTCAATAGGTGGTCCCATCCGCATTCGAACCCTATGCGTAGTTACTCCCGGCAGTAA	LVLNRWSHPHSNPMRSYSRQ*	-1.515	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24817	SLAKLHNFCTVLQSVICVRW			20	SLAY-screened peptide P3167			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCTCGCTAAGCTCCATAACTTCTGTACTGTCTTGCAGTCGGTGATCTGTGTGAGGTGGTAA	SLAKLHNFCTVLQSVICVRW*	-1.515	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24818	SIPCPHLPRCPYPTGPSRGM			20	SLAY-screened peptide P3168			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTATCCCCTGTCCTCACCTCCCCCGGTGCCCTTATCCCACGGGCCCTTCCCGGGGCATGTAA	SIPCPHLPRCPYPTGPSRGM*	-1.515	0.031849	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24819	RSYTLLYNTSEIQIKSVNIR			20	SLAY-screened peptide P3169			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTCTTACACCCTTTTGTATAATACTTCTGAGATTCAGATCAAGTCCGTGAATATCAGGTAA	RSYTLLYNTSEIQIKSVNIR*	-1.515	0.015629	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24820	DLDLICNRFAIVEMLVTTYA			20	SLAY-screened peptide P3170			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCTCGATCTGATCTGTAACCGGTTCGCGATTGTCGAGATGCTGGTTACGACCTACGCGTAA	DLDLICNRFAIVEMLVTTYA*	-1.515	0.008696	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24821	LKQLWRLIGLKLGYRARMYAN			21	SLAY-screened peptide P3171			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAAGCAGTTGTGGAGGCTTATTGGCCTCAAGCTCGGGTATCGTGCGCGCATGTACGCTAAC	LKQLWRLIGLKLGYRARMYAN	-1.515	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24822	PFWLYSDHHWS			11	SLAY-screened peptide P3172			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTCTGGTTGTACTCCGATCATCACTGGAGTTAGCCGTATCACAACCTTAGTAAGACCTAA	PFWLYSDHHWS*PYHNLSKT*	-1.514	0.000136	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24823	PAPALPHRLRRPCK			14	SLAY-screened peptide P3173			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCTCCGGCGCTCCCTCACCGTCTGAGGCGCCCGTGTAAGTAGGGGTACACGACTTTTTAA	PAPALPHRLRRPCK*GYTTF*	-1.514	0.033437	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24824	WFGTTCWFCAIHLDVENPKT			20	SLAY-screened peptide P3174			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTTTGGTACTACTTGTTGGTTCTGCGCCATCCATCTTGACGTCGAGAATCCGAAGACCTAA	WFGTTCWFCAIHLDVENPKT*	-1.514	0.001238	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24825	LHYCLIGLCKPPSPDTCIMK			20	SLAY-screened peptide P3175			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCATTATTGCCTCATTGGTCTGTGTAAGCCTCCTTCCCCTGACACCTGCATCATGAAGTAA	LHYCLIGLCKPPSPDTCIMK*	-1.514	0.016822	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24826	AQSPLLFALRAVFRAILGGLN			21	SLAY-screened peptide P3176			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCAGTCGCCGCTTCTCTTCGCGCTAAGGGCCGTATTCCGTGCAATACTCGGGGGCCTTAAC	AQSPLLFALRAVFRAILGGLN	-1.514	0.043768	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24827	FTRAQAQVTSYWH			13	SLAY-screened peptide P3177			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCACTCGTGCCCAGGCTCAGGTTACCTCCTATTGGCATTAGTAGCCGATTCTGCGCTGCTAA	FTRAQAQVTSYWH**PILRC*	-1.514	0.007912	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24828	DPRHYDTPCIFDRDHSFILT			20	SLAY-screened peptide P3178			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCCCCGTCACTATGACACTCCCTGCATCTTCGATCGTGATCATTCCTTTATTTTGACTTAA	DPRHYDTPCIFDRDHSFILT*	-1.513	0.00046	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24829	TNFNDPTVPQHMNHPMAIKI			20	SLAY-screened peptide P3179			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAACTTTAACGACCCTACGGTTCCCCAGCATATGAACCATCCTATGGCTATTAAGATCTAA	TNFNDPTVPQHMNHPMAIKI*	-1.513	0.007794	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24830	DRNYNDSTH			9	SLAY-screened peptide P3180			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCGCAACTACAATGATTCTACCCACTAGGGTAAGCATACTGACAAGACTCTCTACTCTTAA	DRNYNDSTH*GKHTDKTLYS*	-1.513	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24831	QSLVAVALLLLWTTFRACTFN			21	SLAY-screened peptide P3181			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGAGCCTTGTTGCAGTTGCACTCTTATTACTTTGGACTACTTTTCGCGCCTGTACTTTTAAC	QSLVAVALLLLWTTFRACTFN	-1.513	0.000312	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24832	RQETCISICCGL			12	SLAY-screened peptide P3182			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCAGGAGACCTGTATCAGTATCTGCTGCGGCTTGTAGAGTAAGCTGCTGAATACTAAGTAA	RQETCISICCGL*SKLLNTK*	-1.512	0.036125	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24833	FARGQNQNPGNFTVLFMSKH			20	SLAY-screened peptide P3183			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGCGCGGGGCCAGAACCAGAATCCGGGTAATTTTACCGTCTTGTTCATGTCCAAGCATTAA	FARGQNQNPGNFTVLFMSKH*	-1.512	0.023521	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24834	PNQAALNNGLGGWQFRITVGN			21	SLAY-screened peptide P3184			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAATCAGGCGGCTCTCAATAACGGTCTTGGCGGGTGGCAGTTTCGGATTACCGTAGGTAAC	PNQAALNNGLGGWQFRITVGN	-1.512	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24835	STRPLSIS			8	SLAY-screened peptide P3185			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTACCAGGCCGCTTAGTATCTCGTAGCACCATTAGGTTGTCTCTTTTCATGAGTATTTCTAA	STRPLSIS*HH*VVSFHEYF*	-1.512	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24836	STRADSCPPWLSPFCLFVLR			20	SLAY-screened peptide P3186			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGACTCGGGCTGACTCGTGTCCCCCCTGGTTGAGTCCGTTCTGCCTTTTCGTGCTCCGTTAA	STRADSCPPWLSPFCLFVLR*	-1.511	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24837	FTNVA			5	SLAY-screened peptide P3187			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCACCAACGTTGCCTAGACTCACCCTTCGTCCACGGACCAGCGTTTCCGCGTGGCCAAGTAA	FTNVA*THPSSTDQRFRVAK*	-1.511	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24838	VYCQNPRHVYS			11	SLAY-screened peptide P3188			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTATTGCCAGAATCCGCGGCATGTTTACTCTTAGCATTCTCGGACGCGCCATAACAATTAA	VYCQNPRHVYS*HSRTRHNN*	-1.511	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24839	ITSEDHGYSPHFIYYLVDSI			20	SLAY-screened peptide P3189			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTACTAGCGAGGACCACGGTTATTCCCCGCATTTTATTTACTACCTGGTTGACTCTATTTAA	ITSEDHGYSPHFIYYLVDSI*	-1.511	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24840	PLSHTLPGHQRRASTNPWVH			20	SLAY-screened peptide P3190			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTTCCCATACGCTCCCGGGCCACCAGAGGCGGGCCTCTACGAACCCCTGGGTCCATTAA	PLSHTLPGHQRRASTNPWVH*	-1.511	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24841	MGTNSPAALHNVEVFSILLV			20	SLAY-screened peptide P3191			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGGTACGAATTCGCCCGCCGCCCTCCATAACGTTGAGGTTTTTTCTATTCTCTTGGTTTAA	MGTNSPAALHNVEVFSILLV*	-1.511	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24842	YNGSNLCIAMHNNCHINIYR			20	SLAY-screened peptide P3192			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAATGGGTCCAACTTGTGTATCGCCATGCACAATAACTGTCACATCAACATTTACAGGTAA	YNGSNLCIAMHNNCHINIYR*	-1.511	0.000389	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24843	APC			3	SLAY-screened peptide P3193			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCTTGCTAGACCGTGGACGGGTGCCCGGGTATGCCCTCCCTTCTCAAGTGTTCTTCGTAA	APC*TVDGCPGMPSLLKCSS*	-1.511	0.010338	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24844	TACARRAALYTHHL			14	SLAY-screened peptide P3194			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGCCTGTGCCCGCCGCGCCGCTCTCTACACCCATCACCTGTAGTCTCTGATTACTTCTTAA	TACARRAALYTHHL*SLITS*	-1.511	0.011675	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24845	VDSWNFIASTYRPLNAGPPN			20	SLAY-screened peptide P3195			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGATTCGTGGAATTTTATTGCCAGTACGTACAGGCCTTTGAACGCTGGCCCGCCTAATTAA	VDSWNFIASTYRPLNAGPPN*	-1.511	0.000478	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24846	IVWANLRRFLNLDNTVHRCM			20	SLAY-screened peptide P3196			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGTCTGGGCCAACTTGCGGCGCTTCCTTAATTTGGACAATACCGTGCACCGTTGTATGTAA	IVWANLRRFLNLDNTVHRCM*	-1.51	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24847	YCDIISTAYDKLRTFLMAVR			20	SLAY-screened peptide P3197			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTGCGATATTATCAGCACCGCTTATGACAAGCTTAGGACCTTTCTGATGGCGGTCCGTTAA	YCDIISTAYDKLRTFLMAVR*	-1.51	0.00013	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24848	TTREGPIRVNGLMHLIIDVY			20	SLAY-screened peptide P3198			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGACGCGCGAGGGCCCGATCCGGGTCAACGGGCTCATGCATCTCATTATCGACGTCTACTAA	TTREGPIRVNGLMHLIIDVY*	-1.51	0.000306	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24849	LVKLARFVCNVLRIVRCKCA			20	SLAY-screened peptide P3199			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGTGAAGCTCGCCAGGTTTGTCTGCAATGTTTTGCGCATCGTTCGGTGCAAGTGCGCGTAA	LVKLARFVCNVLRIVRCKCA*	-1.509	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24850	KTTFRITSCVILQYAGVYLS			20	SLAY-screened peptide P3200			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGACCACGTTTCGCATCACGAGCTGCGTCATCCTCCAGTACGCGGGTGTTTATCTTTCGTAA	KTTFRITSCVILQYAGVYLS*	-1.509	0.005297	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24851	CRQDNPSFYFHKITANTFSN			20	SLAY-screened peptide P3201			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCGGCAGGACAACCCTAGCTTCTATTTTCACAAGATTACCGCCAATACGTTCAGTAACTGA	CRQDNPSFYFHKITANTFSN*	-1.509	6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24852	PNCYAMRTTRNSTSSKSCTL			20	SLAY-screened peptide P3202			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAACTGCTACGCTATGCGTACTACCAGGAACTCGACCTCTTCGAAGTCTTGTACGCTCTAA	PNCYAMRTTRNSTSSKSCTL*	-1.509	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24853	CVNTGYARKNPLRACGPFPT			20	SLAY-screened peptide P3203			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGTGAACACCGGCTACGCTCGGAAGAATCCTCTCCGCGCCTGCGGGCCGTTCCCCACTTAA	CVNTGYARKNPLRACGPFPT*	-1.509	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24854	SASLALYQRRNVTQRLRGWS			20	SLAY-screened peptide P3204			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCTTCTCTCGCCCTTTACCAGCGCCGTAATGTTACGCAGCGCCTGCGGGGGTGGTCCTAA	SASLALYQRRNVTQRLRGWS*	-1.508	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24855	FRSRHIGLTCR			11	SLAY-screened peptide P3205			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCGCTCTAGGCATATTGGGCTCACTTGTCGCTAGAACAGTCTTCAGATTCTGAAGATCTAA	FRSRHIGLTCR*NSLQILKI*	-1.508	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24856	SLDASSPHHLAAPGTFAYGS			20	SLAY-screened peptide P3206			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCTGGACGCCTCGTCGCCTCACCATCTCGCCGCCCCTGGCACTTTCGCGTATGGGTCCTAA	SLDASSPHHLAAPGTFAYGS*	-1.508	0.039416	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24857	PYD			3	SLAY-screened peptide P3207			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTACGACTAGGATGTCAACACCGGTACGTTTCGTATTTGCACTTATTCTGCCCGTGGCTAA	PYD*DVNTGTFRICTYSARG*	-1.508	5.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24858	VYCLRCLFRFYSCQLKSPHK			20	SLAY-screened peptide P3208			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTACTGCCTGCGTTGCCTTTTCCGCTTTTATAGCTGCCAGCTGAAGTCTCCTCACAAGTAA	VYCLRCLFRFYSCQLKSPHK*	-1.507	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24859	HMTHLPTRPHLQYNGHSMNV			20	SLAY-screened peptide P3209			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACATGACTCACCTCCCTACGCGGCCGCACCTTCAGTACAATGGCCATTCTATGAACGTTTAA	HMTHLPTRPHLQYNGHSMNV*	-1.507	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24860	AIPTMPTPRCRPFNLVIERT			20	SLAY-screened peptide P3210			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTATTCCCACGATGCCCACTCCTCGTTGCCGGCCTTTCAACCTCGTCATTGAGCGCACTTAA	AIPTMPTPRCRPFNLVIERT*	-1.506	0.000204	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24861	PLHHTILPPS			10	SLAY-screened peptide P3211			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCCATCATACCATCCTGCCTCCTTCGTAGCGTCCTTCGCTTAGTCGTACTGACCCTTAA	PLHHTILPPS*RPSLSRTDP*	-1.506	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24862	QARALCAM			8	SLAY-screened peptide P3212			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGCCCGGGCCTTGTGCGCTATGTAGTGCAGCATTAGGTGGAACCATGATGTGAATCTCTAA	QARALCAM*CSIRWNHDVNL*	-1.506	0.019644	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24863	ALHHCFNYFSAHTWPDRWQK			20	SLAY-screened peptide P3213			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTTGCACCATTGTTTCAATTATTTCAGTGCGCACACCTGGCCTGATCGCTGGCAGAAGTAA	ALHHCFNYFSAHTWPDRWQK*	-1.506	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24864	CHQGRPAHLN			10	SLAY-screened peptide P3214			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCACCAGGGCCGCCCTGCCCATCTCAATTAGTACATTACGAATCACTAGTACTATGGTTAA	CHQGRPAHLN*YITNH*YYG*	-1.506	0.027259	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24865	MIKP			4	SLAY-screened peptide P3215			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGATTAAGCCGTAGCCTGCCCAGGTCAGTGCTGCTACCACTCACTATTACTATTATCCTTAA	MIKP*PAQVSAATTHYYYYP*	-1.506	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24866	LCIFKLILVGMIPISRPWRTN			21	SLAY-screened peptide P3216			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTGCATCTTTAAGCTGATCCTTGTGGGCATGATTCCGATCTCTAGACCATGGAGAACTAAC	LCIFKLILVGMIPISRPWRTN	-1.506	0.000242	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24867	PSDNLHLQILVPITTNYHSD			20	SLAY-screened peptide P3217			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGCGACAATCTGCACCTGCAGATTCTCGTCCCTATCACTACTAATTACCATAGCGATTAA	PSDNLHLQILVPITTNYHSD*	-1.505	0.003437	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24868	RPY			3	SLAY-screened peptide P3218			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCGTATTAGATTAGGGACCTCAAGAATAATCTCTGCTAGACCAACGTTGTCGAGATCTAA	RPY*IRDLKNNLC*TNVVEI*	-1.505	0.000163	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24869	PYQSYPLYSRIKPIFINFCP			20	SLAY-screened peptide P3219			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTACCAGAGTTATCCCCTGTATTCGAGGATCAAGCCCATTTTTATTAACTTCTGCCCGTAA	PYQSYPLYSRIKPIFINFCP*	-1.504	4.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24870	AVVKRSHRCKTGQRAPLL			18	SLAY-screened peptide P3220			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGTTGTCAAGCGTAGTCATAGGTGTAAGACTGGCCAGCGGGCGCCGCTTCTGTAGACTTAA	AVVKRSHRCKTGQRAPLL*T*	-1.504	0.009408	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24871	SV			2	SLAY-screened peptide P3221			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGTCTAGATCCATCTCGGGATTGGTCCGACGAGTAATTACACTTCTGCTGTGGGTGTGTAA	SV*IHLGIGPTSNYTSAVGV*	-1.504	0.002142	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24872	ATNGILHLQIKVTCPLTFGP			20	SLAY-screened peptide P3222			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTACCAACGGCATCTTGCATCTGCAGATTAAGGTTACTTGCCCCTTGACCTTTGGTCCCTAA	ATNGILHLQIKVTCPLTFGP*	-1.503	0.010039	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24873	PWLFDSFVSVIRWLRHRLGH			20	SLAY-screened peptide P3223			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGGTTGTTTGATTCCTTCGTCTCTGTCATTCGTTGGCTGCGCCATCGTCTTGGTCATTAA	PWLFDSFVSVIRWLRHRLGH*	-1.503	0.02103	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24874	PNPKIYNSTIRRTNFTVVSY			20	SLAY-screened peptide P3224			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAACCCCAAGATCTATAATAGTACGATTCGGCGTACCAATTTTACTGTCGTCTCCTATTAA	PNPKIYNSTIRRTNFTVVSY*	-1.503	0.001333	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24875	FPLVI			5	SLAY-screened peptide P3225			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCCCCTCGTTATCTAGCGGAATTACAAGGTTAAGGCTTCTATCCAGCATCACAATAACTAA	FPLVI*RNYKVKASIQHHNN*	-1.503	0.004322	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24876	LPALVIHHHYNIILGGLGRL			20	SLAY-screened peptide P3226			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCGGCTCTGGTGATTCATCACCATTACAATATCATCCTTGGCGGCTTGGGTAGGCTGTAA	LPALVIHHHYNIILGGLGRL*	-1.503	0.049698	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24877	SVTCGSTAACRIHADNSHAP			20	SLAY-screened peptide P3227			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGTGACTTGCGGCTCCACTGCGGCGTGCCGCATTCATGCCGATAACTCGCATGCCCCCTAA	SVTCGSTAACRIHADNSHAP*	-1.502	0.010039	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24878	RLSAQTHSQTSANYRCRLNP			20	SLAY-screened peptide P3228			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTTTCGGCTCAGACTCATAGCCAGACCTCCGCCAACTACCGTTGTCGTCTTAATCCTTAA	RLSAQTHSQTSANYRCRLNP*	-1.502	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24879	LSNALPTILGYDSSTAGLAD			20	SLAY-screened peptide P3229			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTCTAACGCGCTGCCGACTATCCTTGGGTATGACTCCAGCACTGCGGGCCTTGCGGACTAA	LSNALPTILGYDSSTAGLAD*	-1.502	0.005505	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24880	HIRGRLCWITRSPSALMLPCN			21	SLAY-screened peptide P3230			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACATAAGAGGCCGATTATGCTGGATTACACGCTCACCCTCGGCCTTGATGCTGCCCTGTAAC	HIRGRLCWITRSPSALMLPCN	-1.501	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24881	WLSCRVALNFDISRIFIRRY			20	SLAY-screened peptide P3231			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCTTAGCTGTCGCGTTGCGCTTAATTTTGACATCTCTCGGATTTTTATTCGGCGCTATTAA	WLSCRVALNFDISRIFIRRY*	-1.501	0.001227	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24882	RNRRPMANSVSDPIGPSSNS			20	SLAY-screened peptide P3232			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGAACCGCCGGCCGATGGCTAACAGCGTCTCCGACCCCATTGGGCCTTCCTCTAACTCTTAA	RNRRPMANSVSDPIGPSSNS*	-1.501	0.00023	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24883	NPWWFLLQPMYDCTGFHVYS			20	SLAY-screened peptide P3233			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCCGTGGTGGTTTCTTCTCCAGCCTATGTATGACTGCACGGGCTTCCACGTTTATAGTTAA	NPWWFLLQPMYDCTGFHVYS*	-1.501	0.000304	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24884	PRSMGALRPSSVLRDGSH			18	SLAY-screened peptide P3234			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGCAGTATGGGCGCATTACGACCTTCTTCTGTGCTTCGTGATGGATCCCACTAACTAACT	PRSMGALRPSSVLRDGSH*LT	-1.501	0.022443	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24885	LFLDKGHM			8	SLAY-screened peptide P3235			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTTCCTTGACAAGGGTCACATGTAGTTTGTCTTGAACGTTGACATGTCCATTCTTCTCTAA	LFLDKGHM*FVLNVDMSILL*	-1.501	0.000821	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24886	CPVL			4	SLAY-screened peptide P3236			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCTGTGCTGTAGATGCGGCTGCACAACAATTCTTCTTTGCCCTTTTCTGGCATGCGGTAA	CPVL*MRLHNNSSLPFSGMR*	-1.501	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24887	PPAHHDVAQDASCFVNVPGI			20	SLAY-screened peptide P3237			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCGGCTCACCACGACGTTGCGCAGGATGCGTCGTGTTTCGTTAATGTGCCGGGGATTTAA	PPAHHDVAQDASCFVNVPGI*	-1.5	0.002568	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24888	SGPDLTTSCNDNFMNLYYAI			20	SLAY-screened peptide P3238			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGGCCCCGATCTGACCACCAGTTGTAACGATAATTTTATGAACCTGTACTATGCCATCTAA	SGPDLTTSCNDNFMNLYYAI*	-1.5	0.020038	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24889	SARLATRCPLPRAAPRLSRLN			21	SLAY-screened peptide P3239			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGCAAGGCTCGCGACAAGATGTCCATTGCCAAGAGCTGCGCCTAGGCTATCTCGCCTTAAC	SARLATRCPLPRAAPRLSRLN	-1.5	0.00711	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24890	LRATQTPPLPHHLDELHYLA			20	SLAY-screened peptide P3240			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCGGGCGACTCAGACGCCTCCTCTGCCCCATCACCTGGATGAGCTTCACTATCTTGCTTAA	LRATQTPPLPHHLDELHYLA*	-1.5	0.011678	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24891	TSCDMPNDNTIGARAGSGISN			21	SLAY-screened peptide P3241			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAGTTGTGACATGCCCAATGACAACACGATAGGCGCTAGGGCGGGATCGGGAATTTCTAAC	TSCDMPNDNTIGARAGSGISN	-1.5	0.015454	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24892	PAATTLLLRLKRMLSRLAIYY			21	SLAY-screened peptide P3242			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCTGCGACTACCCTTTTGCTCCGTTTGAAGCGCATGTTGTCCCGTCTGGCTATTTACTAC	PAATTLLLRLKRMLSRLAIYY	-1.499	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24893	ATQSSNNPIYADVIPGNVVF			20	SLAY-screened peptide P3243			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACGCAGAGCAGCAACAACCCCATTTATGCTGATGTTATTCCTGGCAACGTTGTTTTCTAA	ATQSSNNPIYADVIPGNVVF*	-1.499	0.000914	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24894	SHLR			4	SLAY-screened peptide P3244			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCACCTCCGTTAGTCAGTCCGATGTCGCTCAACCCGTCGGGCACCGATAGTACTGTGTAAC	SHLR*SVRCRSTRRAPIVLCN	-1.499	0.035867	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24895	LPRMCPYQAIPDSTYTYNYI			20	SLAY-screened peptide P3245			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCGCGTATGTGTCCGTACCAGGCGATTCCCGACTCGACCTATACCTACAACTATATCTAA	LPRMCPYQAIPDSTYTYNYI*	-1.498	0.039106	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24896	RPRYIAASPSGPNETLMTNA			20	SLAY-screened peptide P3246			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCCCGGTATATTGCCGCGAGTCCCTCCGGTCCCAACGAGACGCTTATGACTAACGCTTAA	RPRYIAASPSGPNETLMTNA*	-1.498	0.026332	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24897	GGVAYPPYVIGAFCYPVLPV			20	SLAY-screened peptide P3247			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTGGGGTCGCTTACCCGCCGTATGTGATTGGTGCCTTCTGCTACCCTGTTTTGCCCGTGTAA	GGVAYPPYVIGAFCYPVLPV*	-1.498	0.045223	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24898	PRFSAPSCGCANHPTWHGHM			20	SLAY-screened peptide P3248			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGCTTCTCTGCCCCCTCTTGCGGTTGTGCCAATCACCCTACCTGGCATGGGCACATGTAA	PRFSAPSCGCANHPTWHGHM*	-1.498	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24899	LHSDLCMVCYRINYSRFDYF			20	SLAY-screened peptide P3249			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCATTCGGATCTTTGCATGGTTTGCTATCGTATCAATTATAGCAGGTTTGATTACTTCTAA	LHSDLCMVCYRINYSRFDYF*	-1.498	0.001312	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24900	EPSRRAVVTTASGRIPIEKS			20	SLAY-screened peptide P3250			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCCCAGCAGGCGCGCTGTTGTTACTACCGCCAGTGGGCGTATTCCGATTGAGAAGTCCTAA	EPSRRAVVTTASGRIPIEKS*	-1.498	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24901	KPDAMCSYQASVHDFSYCYL			20	SLAY-screened peptide P3251			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCCTGACGCGATGTGTTCTTACCAGGCGAGCGTCCATGACTTTTCGTACTGCTATTTGTAA	KPDAMCSYQASVHDFSYCYL*	-1.498	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24902	DYPNTYEFMPYNRWCYRAFM			20	SLAY-screened peptide P3252			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTATCCTAACACCTACGAGTTTATGCCGTATAACCGTTGGTGCTATAGGGCTTTCATGTAA	DYPNTYEFMPYNRWCYRAFM*	-1.497	0.001323	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24903	PSSALFSLGSNVGIPPDVPL			20	SLAY-screened peptide P3253			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCTTCGGCTCTCTTTTCCCTCGGGTCTAACGTGGGCATCCCTCCGGATGTTCCGCTGTAA	PSSALFSLGSNVGIPPDVPL*	-1.497	9.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24904	TAYQLTNLVVGSNFFTLDSV			20	SLAY-screened peptide P3254			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGCTTATCAGTTGACCAATTTGGTGGTTGGTAGTAATTTCTTTACCCTTGACTCTGTGTAA	TAYQLTNLVVGSNFFTLDSV*	-1.497	0.000641	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24905	CVTVAPQNNAYDDTYKYYPV			20	SLAY-screened peptide P3255			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGTTACCGTGGCCCCTCAGAACAATGCCTATGATGATACTTACAAGTATTATCCTGTGTAA	CVTVAPQNNAYDDTYKYYPV*	-1.497	0.002077	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24906	CLDDVHIGRAMPTWIAFTVH			20	SLAY-screened peptide P3256			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTGGATGATGTCCACATCGGTCGGGCTATGCCTACTTGGATTGCTTTTACTGTGCACTAA	CLDDVHIGRAMPTWIAFTVH*	-1.497	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24907	CS			2	SLAY-screened peptide P3257			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTCGTAGCATTGTCTCTTGTTCAAGGATCACCATATGTTTGTTACTTTTTGGACCATCTAA	CS*HCLLFKDHHMFVTFWTI*	-1.496	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24908	NTNCTNTPWPRIYTDYAGFL			20	SLAY-screened peptide P3258			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACACCAATTGTACTAACACGCCCTGGCCGAGGATCTACACGGATTATGCTGGTTTCCTTTAA	NTNCTNTPWPRIYTDYAGFL*	-1.496	0.013085	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24909	PGHFFYVPCIQIDTLNHSSR			20	SLAY-screened peptide P3259			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGGTCATTTCTTTTATGTCCCGTGCATCCAGATTGATACCTTGAACCATTCTAGCCGTTAA	PGHFFYVPCIQIDTLNHSSR*	-1.496	0.000224	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24910	ALESPRRRCPPIFLQQEYEP			20	SLAY-screened peptide P3260			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCTCGAGTCCCCTCGTCGCCGGTGTCCGCCGATTTTCCTTCAGCAGGAGTACGAGCCCTAA	ALESPRRRCPPIFLQQEYEP*	-1.496	0.034066	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24911	RAPLAFVLLLHKRGTTHRPD			20	SLAY-screened peptide P3261			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGCGCCCTTGGCTTTTGTTTTGCTGCTTCATAAGCGTGGTACCACCCACCGTCCCGATTAA	RAPLAFVLLLHKRGTTHRPD*	-1.496	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24912	RMRCPTNLDYKSHDGLCHAF			20	SLAY-screened peptide P3262			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATGCGCTGCCCCACTAACCTCGACTATAAGTCTCACGATGGCCTCTGCCACGCTTTTTAA	RMRCPTNLDYKSHDGLCHAF*	-1.496	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24913	TYAIHRCLSFARHTLDNHTI			20	SLAY-screened peptide P3263			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTACGCTATCCACCGGTGCCTTTCCTTTGCGCGGCATACTCTTGACAATCATACTATTTAA	TYAIHRCLSFARHTLDNHTI*	-1.496	2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24914	FRQHCIPLIYLTDYDFHNKA			20	SLAY-screened peptide P3264			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCGGCAGCATTGCATCCCCCTCATCTACTTGACCGATTATGACTTTCACAACAAGGCGTAA	FRQHCIPLIYLTDYDFHNKA*	-1.495	0.0122	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24915	ERFFLSYCGYTSRDVCFYPS			20	SLAY-screened peptide P3265			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCGGTTCTTCCTGTCGTATTGCGGGTATACTTCGCGGGATGTCTGCTTCTATCCTAGTTAA	ERFFLSYCGYTSRDVCFYPS*	-1.495	0.001355	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24916	PAGMLLATAFPNTSMWHYPH			20	SLAY-screened peptide P3266			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCTGGCATGCTTCTTGCGACTGCTTTCCCCAATACTTCGATGTGGCATTACCCTCACTAA	PAGMLLATAFPNTSMWHYPH*	-1.495	0.000535	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24917	DCVVATTDRANKKIPLAYSH			20	SLAY-screened peptide P3267			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTGTGTTGTTGCGACGACCGATAGGGCCAACAAGAAGATCCCTCTTGCTTATTCTCACTAA	DCVVATTDRANKKIPLAYSH*	-1.495	0.000797	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24918	LDSYLKVYPPVTTKTDPMSR			20	SLAY-screened peptide P3268			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGACAGCTACCTTAAGGTCTACCCTCCTGTTACTACGAAGACGGATCCGATGTCTCGTTAA	LDSYLKVYPPVTTKTDPMSR*	-1.495	0.031086	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24919	CLYHISDS			8	SLAY-screened peptide P3269			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCTTTATCATATTTCTGACAGCTAGAACATTATTAAGCTCTCGCGTTCCACTAGTTGCTAA	CLYHISDS*NIIKLSRSTSC*	-1.495	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24920	AGPSPCQSWTTRSIISVRTSN			21	SLAY-screened peptide P3270			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGGCCCGTCTCCCTGCCAGAGTTGGACGACACGGTCAATTATTAGCGTCCGGACTTCTAAC	AGPSPCQSWTTRSIISVRTSN	-1.494	0.000308	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24921	HDSSMSRPWV			10	SLAY-screened peptide P3271			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGATTCCTCTATGTCTCGCCCGTGGGTTTAGTATCATCATGTTGACCTTAATTCTATGTAA	HDSSMSRPWV*YHHVDLNSM*	-1.494	0.002934	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24922	LMRWSVRTHRSHPSHKRGPH			20	SLAY-screened peptide P3272			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGATGCGCTGGAGTGTCCGTACGCACAGGAGTCATCCGAGCCACAAGAGGGGTCCTCATTAA	LMRWSVRTHRSHPSHKRGPH*	-1.494	0.000164	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24923	IHSITTFSYSDISLASPSTC			20	SLAY-screened peptide P3273			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCACTCTATCACCACCTTCAGTTACAGTGATATCAGCTTGGCCAGTCCCTCCACTTGTTAA	IHSITTFSYSDISLASPSTC*	-1.493	0.003566	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24924	PRSLLRANYDSIDTVYTSTA			20	SLAY-screened peptide P3274			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAGGAGCCTCCTCAGGGCCAACTACGATTCTATCGATACCGTTTACACGTCTACCGCGTAA	PRSLLRANYDSIDTVYTSTA*	-1.493	0.013286	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24925	AVTVTCACHTYCYFQQDRHI			20	SLAY-screened peptide P3275			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGTGACCGTGACCTGTGCCTGTCATACCTATTGCTACTTCCAGCAGGATAGGCATATCTAA	AVTVTCACHTYCYFQQDRHI*	-1.493	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24926	CGLTNDEARYCGSSFCSISTN			21	SLAY-screened peptide P3276			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGGTCTCACTAATGACGAGGCCAGGTACTGCGGGTCCAGTTTTTGTTCAATATCAACTAAC	CGLTNDEARYCGSSFCSISTN	-1.492	0.000267	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24927	PGSYVFAPLVLVWPILSVRT			20	SLAY-screened peptide P3277			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGCTCCTACGTGTTCGCCCCTCTGGTTCTGGTCTGGCCGATTCTGTCTGTCCGTACTTAA	PGSYVFAPLVLVWPILSVRT*	-1.492	0.00348	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24928	STMPGSTLHGHCHIFPVSSI			20	SLAY-screened peptide P3278			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTACCATGCCCGGCAGCACTCTTCACGGCCACTGTCACATCTTCCCCGTGTCCAGTATTTAA	STMPGSTLHGHCHIFPVSSI*	-1.492	0.003319	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24929	PSLTPLCCPDDLKPRQVWLT			20	SLAY-screened peptide P3279			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCCCTTACGCCCCTCTGTTGCCCCGATGACCTTAAGCCCAGGCAGGTTTGGCTCACGTAA	PSLTPLCCPDDLKPRQVWLT*	-1.492	0.001706	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24930	NTNTLTFSHASDLDHTGTLY			20	SLAY-screened peptide P3280			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATACTAATACGCTCACCTTTAGCCACGCCTCTGACCTGGACCACACCGGCACTCTGTATTAA	NTNTLTFSHASDLDHTGTLY*	-1.492	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24931	CKINNTTLDARYHPSNTVAN			20	SLAY-screened peptide P3281			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAAGATTAATAATACTACGCTGGACGCTAGGTATCACCCCTCGAACACGGTCGCTAATTAA	CKINNTTLDARYHPSNTVAN*	-1.492	0.037241	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24932	CRYAHCVSLFPNASFINQVV			20	SLAY-screened peptide P3282			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCGCTACGCCCACTGCGTTAGCTTGTTTCCCAACGCGAGTTTTATCAACCAGGTGGTGTAA	CRYAHCVSLFPNASFINQVV*	-1.492	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24933	WSPLDVILAGNHPHSGTLWI			20	SLAY-screened peptide P3283			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTCCCCCCTTGATGTTATTCTCGCTGGCAACCACCCCCATAGTGGGACGCTGTGGATCTAA	WSPLDVILAGNHPHSGTLWI*	-1.491	0.031237	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24934	PANIDGWPRRAYCVKSISDT			20	SLAY-screened peptide P3284			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGCTAATATCGATGGCTGGCCGAGGCGCGCTTACTGTGTCAAGAGCATTTCGGATACCTAA	PANIDGWPRRAYCVKSISDT*	-1.491	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24935	HCCAKYQLSPSSDVCFYNRS			20	SLAY-screened peptide P3285			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGCTGTGCTAAGTACCAGCTTTCGCCTAGTAGTGATGTGTGTTTTTATAACCGCTCTTAA	HCCAKYQLSPSSDVCFYNRS*	-1.491	0.013837	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24936	TTPLSNWLDSVLDTPATTIR			20	SLAY-screened peptide P3286			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACCCCCCTTTCGAACTGGTTGGACTCTGTGCTGGACACTCCTGCTACGACGATCCGTTAA	TTPLSNWLDSVLDTPATTIR*	-1.491	0.005491	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24937	ASKCVRLTFALIVINVYKGN			20	SLAY-screened peptide P3287			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTCCAAGTGTGTTCGCCTTACTTTCGCTCTTATTGTGATTAACGTGTACAAGGGTAATTAA	ASKCVRLTFALIVINVYKGN*	-1.49	0.007762	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24938	TNYLPGLQKSYVLHYATNIV			20	SLAY-screened peptide P3288			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAACTATTTGCCCGGCCTCCAGAAGAGTTATGTGCTTCATTATGCTACCAATATTGTTTAA	TNYLPGLQKSYVLHYATNIV*	-1.49	0.003317	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24939	IPSYLNFFLAPHCYYTTHFL			20	SLAY-screened peptide P3289			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCCGTCGTACTTGAACTTTTTCTTGGCGCCCCATTGTTATTACACTACTCACTTCTTGTAA	IPSYLNFFLAPHCYYTTHFL*	-1.49	0.012829	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24940	SANDFNGYPTGIVQYIFCRP			20	SLAY-screened peptide P3290			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCGAATGATTTTAACGGTTACCCCACGGGCATCGTCCAGTATATCTTCTGCCGTCCCTAA	SANDFNGYPTGIVQYIFCRP*	-1.49	0.000482	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24941	RCAHTARFPELTVSTPEMETY			21	SLAY-screened peptide P3291			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGCGCGCACACGGCTCGTTTTCCTGAGCTTACGGTTTCCACCCCTGAGATGGAGACTTAC	RCAHTARFPELTVSTPEMETY	-1.489	0.00038	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24942	TTRCGSHNSNPYIALPQLNI			20	SLAY-screened peptide P3292			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACCCGTTGTGGCTCCCACAACTCGAATCCGTATATTGCGCTGCCTCAGTTGAACATCTAA	TTRCGSHNSNPYIALPQLNI*	-1.489	0.020396	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24943	MSNYITRYQNLGTLWSPDNV			20	SLAY-screened peptide P3293			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTCCAATTACATTACCCGCTATCAGAATCTCGGTACTCTGTGGTCCCCTGACAATGTCTAA	MSNYITRYQNLGTLWSPDNV*	-1.489	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24944	SSFDKPYVYSLLLGIPYTCY			20	SLAY-screened peptide P3294			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTCCTTTGATAAGCCCTACGTTTACTCCTTGCTTCTTGGCATTCCCTATACGTGTTACTAA	SSFDKPYVYSLLLGIPYTCY*	-1.489	0.006986	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24945	IACFLDPGAHFPMMDQNTNL			20	SLAY-screened peptide P3295			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGCTTGCTTTTTGGATCCTGGCGCCCATTTTCCCATGATGGATCAGAACACCAACCTTTAA	IACFLDPGAHFPMMDQNTNL*	-1.489	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24946	SCQAPPPNIWTRYVLTASIL			20	SLAY-screened peptide P3296			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTGTCAGGCTCCGCCGCCCAATATCTGGACCCGGTATGTCCTCACCGCTAGTATCTTGTAA	SCQAPPPNIWTRYVLTASIL*	-1.488	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24947	YPACCQMPTSNTWCPLWKAF			20	SLAY-screened peptide P3297			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCCTGCTTGTTGCCAGATGCCCACCAGTAACACGTGGTGTCCTTTGTGGAAGGCCTTTTAA	YPACCQMPTSNTWCPLWKAF*	-1.488	0.006871	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24948	TTPSSYSPWRRWLHFFS			17	SLAY-screened peptide P3298			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTACCCCTAGTTCCTATTCTCCCTGGCGCCGTTGGCTTCACTTTTTTTCGTAGCTCGAGTAA	TTPSSYSPWRRWLHFFS*LE*	-1.488	0.005469	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24949	SHVTGIPNTGHPSRSALANV			20	SLAY-screened peptide P3299			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCACGTCACCGGCATTCCTAATACGGGCCACCCGTCCCGGAGTGCCTTGGCTAATGTTTAA	SHVTGIPNTGHPSRSALANV*	-1.488	0.002882	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24950	HVPT			4	SLAY-screened peptide P3300			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGTGCCGACCTAGCCGTCCCTCAACACGCACACTCATAATTCTAATAACCACGAGGGTTAA	HVPT*PSLNTHTHNSNNHEG*	-1.487	0.03846	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24951	LRSTMTGSETLAVTHLSKSTC			21	SLAY-screened peptide P3301			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCGGAGCACTATGACTGGTTCCGAAACCCTGGCCGTAACCCATTTGAGTAAGTCGACCTGC	LRSTMTGSETLAVTHLSKSTC	-1.486	0.002414	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24952	HPPTLCRAHKRYNRS			15	SLAY-screened peptide P3302			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCCCCCACGCTCTGCCGTGCCCACAAGCGCTACAATCGTTCTTAGGGGCCGCTTCTGTAA	HPPTLCRAHKRYNRS*GPLL*	-1.486	0.000438	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24953	FWCVPPFSLNLHRALYYWSL			20	SLAY-screened peptide P3303			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTGGTGTGTCCCCCCGTTTTCTCTTAACTTGCACCGTGCTCTCTATTATTGGTCCTTGTAA	FWCVPPFSLNLHRALYYWSL*	-1.485	0.00014	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24954	PLCALLKILVYYRINHCKRT			20	SLAY-screened peptide P3304			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTTGTGCGCTCCTTAAGATCCTTGTCTACTACCGTATTAACCATTGCAAGCGGACCTAA	PLCALLKILVYYRINHCKRT*	-1.485	0.000623	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24955	APVHSVADVAYLSCFVLEPQ			20	SLAY-screened peptide P3305			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCGGTGCATAGCGTTGCTGATGTCGCTTATCTGTCGTGCTTTGTACTTGAACCGCAATAA	APVHSVADVAYLSCFVLEPQ*	-1.485	0.001485	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24956	RYTGHAWSAQNKFNLMYVVL			20	SLAY-screened peptide P3306			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTATACCGGCCACGCCTGGTCTGCTCAGAACAAGTTTAATCTTATGTATGTTGTTCTCTAA	RYTGHAWSAQNKFNLMYVVL*	-1.485	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24957	LSIESSVM			8	SLAY-screened peptide P3307			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTCGATCGAGTCTTCTGTCATGTAGCCCCAGGTCGTAGCATGAGGTCGATGATATGGGTTA	LSIESSVM*PQVVA*GR*YGL	-1.484	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24958	HATNSYVLNESWPYSSH			17	SLAY-screened peptide P3308			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGCTACGAACAGTTATGTGCTCAACGAGTCCTGGCCCTATTCCAGCCACTAGCACCCCTAA	HATNSYVLNESWPYSSH*HP*	-1.484	0.000165	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24959	CTAQNFKYL			9	SLAY-screened peptide P3309			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGGTGTACTGCCCAGAACTTTAAGTATCTTTAGCGTCGCACTGGCATCAATTTTAAGTAACTGAGT	CTAQNFKYL*RRTGINFK*LS	-1.483	0.039132	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24960	FVFSLVVSTPVLSICISILPN			21	SLAY-screened peptide P3310			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGTATTCTCCCTAGTAGTGAGCACCCCAGTTTTGTCCATATGCATCAGCATTTTGCCTAAC	FVFSLVVSTPVLSICISILPN	-1.483	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24961	NRLATHTSFLPVSLRPGRGC			20	SLAY-screened peptide P3311			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCGCCTCGCCACGCACACTTCTTTTCTCCCCGTCAGCTTGCGCCCTGGCCGCGGCTGTTAA	NRLATHTSFLPVSLRPGRGC*	-1.483	0.000335	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24962	CRPLEWKQPFQVYGL			15	SLAY-screened peptide P3312			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAGGCCCCTGGAGTGGAAGCAGCCTTTTCAGGTCTATGGCTTGTAGAATTTCAGCAATTAA	CRPLEWKQPFQVYGL*NFSN*	-1.483	0.006635	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24963	QNLYLLSIPTTYFFVSSMLW			20	SLAY-screened peptide P3313			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGAATTTGTACCTCCTTTCTATTCCCACGACTTATTTTTTCGTTAGCTCCATGCTGTGGTAA	QNLYLLSIPTTYFFVSSMLW*	-1.483	0.001318	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24964	HCFWDRYFFNAPTYLHVRNV			20	SLAY-screened peptide P3314			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTGTTTCTGGGACCGCTACTTTTTCAATGCCCCTACGTATCTTCACGTGCGCAATGTTTAA	HCFWDRYFFNAPTYLHVRNV*	-1.483	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24965	LSLYYQTTSVLVVTYTTKIH			20	SLAY-screened peptide P3315			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTCCCTTTACTACCAGACGACGTCCGTTCTCGTTGTCACTTACACCACCAAGATCCACTAA	LSLYYQTTSVLVVTYTTKIH*	-1.482	0.003488	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24966	GCTRTGTIPYYLYAAYTMSN			20	SLAY-screened peptide P3316			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTGCACGCGTACTGGTACTATTCCCTACTACCTCTATGCCGCCTACACTATGAGCAACTAA	GCTRTGTIPYYLYAAYTMSN*	-1.482	0.000197	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24967	SRRCITVPTQ			10	SLAY-screened peptide P3317			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCGCCGTTGTATCACGGTTCCCACCCAGTAGTATTACGTCCGCGATGACGTGGTTCCGTAA	SRRCITVPTQ*YYVRDDVVP*	-1.482	0.000153	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24968	SNLVPCHTISPNNTYITTAL			20	SLAY-screened peptide P3318			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCAACCTCGTGCCTTGTCATACCATTAGCCCCAATAATACCTACATCACTACCGCGTTGTAA	SNLVPCHTISPNNTYITTAL*	-1.481	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24969	GGRCTYPPSLGSTPVITSII			20	SLAY-screened peptide P3319			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGGTCGGTGCACCTACCCTCCTAGTCTTGGTAGTACCCCGGTGATTACCAGTATTATTTAA	GGRCTYPPSLGSTPVITSII*	-1.481	0.016676	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24970	SNSIGKLSAVDSSCSYCISSN			21	SLAY-screened peptide P3320			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGAATTCCATTGGCAAGCTTAGTGCCGTCGATAGCTCTTGCAGCTATTGCATAAGCTCTAAC	SNSIGKLSAVDSSCSYCISSN	-1.481	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24971	NAGHGPCGVKHLI			13	SLAY-screened peptide P3321			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGCCGGGCACGGCCCCTGTGGCGTTAAGCACCTTATTTAGTCGTGCCGTACTTTTAGTTAA	NAGHGPCGVKHLI*SCRTFS*	-1.481	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24972	PLSPFTSHSSSTKRAHGAE			19	SLAY-screened peptide P3322			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTGTCCCCTTTCACCTCGCATTCGTCCTCGACTAAGCGTGCTCATGGTGCTGAGTAAGTC	PLSPFTSHSSSTKRAHGAE*V	-1.48	0.00264	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24973	SVTPIYSPHPDYTST			15	SLAY-screened peptide P3323			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGTGACCCCCATTTACAGCCCCCACCCGGATTACACGTCTACGTAGGTGGATGTGGACTAA	SVTPIYSPHPDYTST*VDVD*	-1.48	5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24974	CIGPKRCLPEDYKSRRPMCPN			21	SLAY-screened peptide P3324			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCATCGGTCCCAAGCGCTGCCTCCCTGAGGACTACAAGAGTCGGAGGCCGATGTGCCCTAAC	CIGPKRCLPEDYKSRRPMCPN	-1.48	0.044403	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24975	GPRLSAFTNWYLLYIRPVID			20	SLAY-screened peptide P3325			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCCCCGCCTGTCGGCCTTTACTAACTGGTACCTTCTGTATATTCGCCCCGTGATCGACTAA	GPRLSAFTNWYLLYIRPVID*	-1.479	0.001977	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24976	TDADGGSWLPFQRHPHNDEQ			20	SLAY-screened peptide P3326			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGACGCCGACGGCGGGTCGTGGCTGCCCTTTCAGAGGCACCCGCATAATGACGAGCAGTAA	TDADGGSWLPFQRHPHNDEQ*	-1.479	0.000844	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24977	NGDPSQFSNPAHFIAVYSDT			20	SLAY-screened peptide P3327			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGGCGACCCGTCCCAGTTTAGTAATCCTGCTCATTTCATTGCCGTTTATAGTGACACTTAA	NGDPSQFSNPAHFIAVYSDT*	-1.479	0.042171	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24978	VACFKRSNPAYALVSLMSALN			21	SLAY-screened peptide P3328			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGCCTGCTTTAAGCGCTCGAATCCCGCGTATGCGTTAGTTAGCCTTATGTCTGCACTTAAC	VACFKRSNPAYALVSLMSALN	-1.479	0.00797	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24979	YAPCATFNLQPFPVITT			17	SLAY-screened peptide P3329			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGCGCCTTGTGCTACGTTTAACCTTCAGCCGTTCCCTGTGATTACGACTTAGAATGCCTAA	YAPCATFNLQPFPVITT*NA*	-1.479	0.004815	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24980	PTLQAQSHSNKS			12	SLAY-screened peptide P3330			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACCCTGCAGGCCCAGTCCCATAGCAATAAGAGTTAGGCTACCCACTAGTCTCCCCCGTAA	PTLQAQSHSNKS*ATH*SPP*	-1.478	0.00384	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24981	GARPSSTRSGLLPSRIFLGFN			21	SLAY-screened peptide P3331			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGCTAGACCGTCATCTACACGGAGCGGTCTATTGCCCAGTAGGATTTTTCTCGGTTTTAAC	GARPSSTRSGLLPSRIFLGFN	-1.478	0.045295	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24982	TYATTTNIIGHLRFGLWYTG			20	SLAY-screened peptide P3332			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTACGCCACTACCACGAACATTATTGGCCATCTGCGTTTCGGCCTGTGGTACACCGGCTAA	TYATTTNIIGHLRFGLWYTG*	-1.478	0.000158	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24983	PNSFSIVYCGNTDLKYRTNS			20	SLAY-screened peptide P3333			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAATAGCTTCAGCATTGTTTACTGTGGGAACACCGATTTGAAGTACCGCACCAATAGTTAA	PNSFSIVYCGNTDLKYRTNS*	-1.478	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24984	TVNRVTFLSHLDPRLTPYRR			20	SLAY-screened peptide P3334			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTGAATCGGGTTACCTTCTTGTCTCATCTTGACCCTCGCCTGACGCCGTATAGGAGGTAA	TVNRVTFLSHLDPRLTPYRR*	-1.478	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24985	LKSHTGLPVSTHVSMLNYIN			20	SLAY-screened peptide P3335			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAAGTCCCATACGGGCCTGCCTGTGTCCACTCACGTTAGTATGTTGAACTACATCAACTAA	LKSHTGLPVSTHVSMLNYIN*	-1.477	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24986	CAGSHILGTRSPRRCCRGLIN			21	SLAY-screened peptide P3336			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCCGGTTCTCATATCCTTGGCACCCGGTCACCACGAAGGTGTTGCCGAGGCCTCATTAAC	CAGSHILGTRSPRRCCRGLIN	-1.477	0.010536	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24987	PKTASCVALPAILPRLAWVGN			21	SLAY-screened peptide P3337			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAAAACCGCGAGTTGCGTCGCTCTACCTGCCATACTGCCACGACTTGCCTGGGTGGGTAAC	PKTASCVALPAILPRLAWVGN	-1.477	0.006275	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24988	LRIPNCSIAFNFHIHKRVMQ			20	SLAY-screened peptide P3338			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGAGGATCCCCAATTGTTCTATTGCTTTTAATTTTCACATTCACAAGCGGGTTATGCAGTAA	LRIPNCSIAFNFHIHKRVMQ*	-1.476	0.003902	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24989	PSTISPIPKVLLHKTSLTDI			20	SLAY-screened peptide P3339			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCGACCATCAGTCCCATTCCTAAGGTGCTTCTCCACAAGACTTCGCTTACGGATATCTAA	PSTISPIPKVLLHKTSLTDI*	-1.476	0.001248	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24990	RTFPSWRRSSPISWPYRYTT			20	SLAY-screened peptide P3340			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGACGTTTCCCAGTTGGAGGCGCTCGAGTCCCATTTCTTGGCCCTATCGTTACACCACGTAA	RTFPSWRRSSPISWPYRYTT*	-1.476	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24991	HSPHFDALYNMYIFVSGSLF			20	SLAY-screened peptide P3341			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTCTCCGCACTTTGACGCGCTTTACAATATGTACATTTTTGTCAGCGGTTCGTTGTTCTAA	HSPHFDALYNMYIFVSGSLF*	-1.476	4.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24992	DDGFGSKITFV			11	SLAY-screened peptide P3342			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATGACGGTTTCGGGAGCAAGATCACGTTTGTTTAGCGGTCTGTTCCTGTTCGCGCGTACTAA	DDGFGSKITFV*RSVPVRAY*	-1.476	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24993	SLGRFCPVVELPYLIAHGRR			20	SLAY-screened peptide P3343			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCTCGGGCGGTTTTGCCCTGTGGTTGAGCTGCCGTATCTTATTGCGCACGGGCGGCGCTAA	SLGRFCPVVELPYLIAHGRR*	-1.476	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24994	PYVPHNPPNAAILALPFSSN			20	SLAY-screened peptide P3344			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTATGTGCCCCACAATCCGCCGAATGCGGCGATCCTGGCGCTACCGTTCAGCTCTAACTGA	PYVPHNPPNAAILALPFSSN*	-1.476	0.000504	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24995	TCVFQPTNPPSMPYTIIQTT			20	SLAY-screened peptide P3345			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTGCGTCTTTCAGCCGACTAACCCGCCCTCGATGCCTTACACCATCATCCAGACTACTTAA	TCVFQPTNPPSMPYTIIQTT*	-1.476	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24996	PTGLRTLLSYSWLRDDLRRS			20	SLAY-screened peptide P3346			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACGGGCTTGAGGACCCTGCTTTCCTATAGTTGGCTCCGTGACGATCTTCGTCGCTCCTAA	PTGLRTLLSYSWLRDDLRRS*	-1.476	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24997	CYTPDYGRPPQTLPLTTFLF			20	SLAY-screened peptide P3347			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTACACTCCTGATTACGGCAGGCCTCCGCAGACGCTCCCCCTGACCACTTTCCTTTTTTAA	CYTPDYGRPPQTLPLTTFLF*	-1.475	0.01068	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24998	TQGTRTTYPASPCPDKWHTT			20	SLAY-screened peptide P3348			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCAGGGCACGCGCACCACTTACCCTGCCTCTCCCTGTCCCGACAAGTGGCACACCACCTAA	TQGTRTTYPASPCPDKWHTT*	-1.475	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP24999	FYTLRTVAARNNLPFRLVRGN			21	SLAY-screened peptide P3349			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTATACTTTGCGGACTGTTGCTGCTAGGAACAACCTTCCTTTTAGACTAGTGCGGGGTAAC	FYTLRTVAARNNLPFRLVRGN	-1.475	0.02813	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25000	HNRCRALCNFA			11	SLAY-screened peptide P3350			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAACCGCTGTAGGGCTCTCTGTAACTTCGCTTAGTCCATCTCTAATAGCCCTCACAGTTAA	HNRCRALCNFA*SISNSPHS*	-1.475	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25001	NRFQLAIAYALFTTTTLIHP			20	SLAY-screened peptide P3351			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCGTTTTCAGCTCGCCATTGCGTATGCTTTGTTTACTACCACTACCCTCATTCATCCCTAA	NRFQLAIAYALFTTTTLIHP*	-1.474	4.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25002	HMKVARHKMPNHPSFFNHDSY			21	SLAY-screened peptide P3352			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACATGAAGGTGGCCCGCCACAAGATGCCCAACCATCCTAGCTTTTTCAACCATGATAGCTAC	HMKVARHKMPNHPSFFNHDSY	-1.474	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25003	SLVPKFNNFSICMSLPPSIIN			21	SLAY-screened peptide P3353			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTTGGTTCCCAAGTTTAATAACTTTAGTATCTGCATGAGCCTTCCCCCATCCATAATTAAC	SLVPKFNNFSICMSLPPSIIN	-1.474	0.048555	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25004	ASNSDASLMPDCPGHIHAHG			20	SLAY-screened peptide P3354			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTAGCAACTCCGACGCGTCGCTCATGCCTGATTGTCCTGGTCACATCCACGCTCACGGTTAA	ASNSDASLMPDCPGHIHAHG*	-1.474	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25005	FTIMVTITVVFSYWDTTPNP			20	SLAY-screened peptide P3355			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTACCATTATGGTCACTATCACTGTGGTGTTCAGCTATTGGGATACTACCCCTAATCCTTAA	FTIMVTITVVFSYWDTTPNP*	-1.474	2.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25006	NMSIMRHKSLR			11	SLAY-screened peptide P3356			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATATGTCCATTATGCGCCACAAGAGTCTTCGTTAGGCCCAGGCGACTATTAATTACATGTAA	NMSIMRHKSLR*AQATINYM*	-1.474	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25007	RD			2	SLAY-screened peptide P3357			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGACTAGAGGGTCCCCTCGCATTGGAGCGTTGTCTGGATCAATAGGTCGGTGCCGACGTAA	RD*RVPSHWSVVWINRSVPT*	-1.473	0.003299	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25008	PRVRLSPHTDCKNDVCLIAV			20	SLAY-screened peptide P3358			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGTGTCCGCCTCAGTCCCCATACCGACTGCAAGAATGACGTCTGTCTCATCGCGGTGTAA	PRVRLSPHTDCKNDVCLIAV*	-1.473	0.010727	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25009	SPCAICLWWLNLFIATNRKT			20	SLAY-screened peptide P3359			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCCGTGCGCTATTTGTTTGTGGTGGCTTAATCTTTTCATCGCCACGAATCGGAAGACGTAA	SPCAICLWWLNLFIATNRKT*	-1.473	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25010	TYTNWHHWTPFILYQACRAS			20	SLAY-screened peptide P3360			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTATACTAATTGGCATCACTGGACCCCTTTTATTCTGTACCAGGCTTGCCGGGCTTCGTAA	TYTNWHHWTPFILYQACRAS*	-1.472	0.037515	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25011	PKFATPTCNHIVRAVIAINY			20	SLAY-screened peptide P3361			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAAGTTCGCTACGCCGACCTGTAACCACATCGTTCGGGCTGTTATCGCTATCAATTACTAA	PKFATPTCNHIVRAVIAINY*	-1.472	0.001286	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25012	DHVQVAWRPVTDSAHSRGAH			20	SLAY-screened peptide P3362			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCACGTCCAGGTCGCCTGGCGCCCGGTTACCGATTCTGCGCACAGCCGTGGCGCCCACTAA	DHVQVAWRPVTDSAHSRGAH*	-1.472	0.004909	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25013	LCDTLVFPMTNDPLYTRARLN			21	SLAY-screened peptide P3363			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTGCGATACTTTGGTTTTCCCCATGACCAATGATCCCTTGTACACTAGAGCACGACTTAAC	LCDTLVFPMTNDPLYTRARLN	-1.472	0.014428	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25014	SRNLHESDYTCSYPTPGNGA			20	SLAY-screened peptide P3364			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCGCAACCTCCACGAGAGCGACTATACGTGCTCCTATCCCACCCCGGGGAACGGTGCCTAA	SRNLHESDYTCSYPTPGNGA*	-1.472	0.037832	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25015	PSKPCAAYYTTKNWSAHTGS			20	SLAY-screened peptide P3365			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCCAAGCCGTGCGCGGCTTATTATACCACTAAGAACTGGTCCGCGCATACTGGCAGCTAA	PSKPCAAYYTTKNWSAHTGS*	-1.472	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25016	GGSFVVGLLTRQDTKLFQHS			20	SLAY-screened peptide P3366			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGGTAGTTTTGTCGTGGGGCTTCTGACCCGGCAGGATACCAAGCTTTTCCAGCATTCGTAA	GGSFVVGLLTRQDTKLFQHS*	-1.472	0.011981	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25017	FMFG			4	SLAY-screened peptide P3367			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCATGTTTGGTTAGGTCAAGTCCCTCTCGAGGCAGGGTTCCTTGTTCGTCGATATGTACTAA	FMFG*VKSLSRQGSLFVDMY*	-1.471	0.000702	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25018	DNQDATINIYRAFIFRLINLT			21	SLAY-screened peptide P3368			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATAATCAGGATGCTACGATTAACATCTACCGTGCGTTCATCTTTAGGCTTATCAACTTAACT	DNQDATINIYRAFIFRLINLT	-1.471	0.01245	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25019	RAPCSHRTGGFLPSR			15	SLAY-screened peptide P3369			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGCCCCCTGTAGTCACCGCACGGGGGGCTTTCTTCCTAGTAGGTAGATGTTCAAGCTTTAA	RAPCSHRTGGFLPSR*MFKL*	-1.471	0.007514	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25020	PEAGSVQIHNFEVLPCHFLV			20	SLAY-screened peptide P3370			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGAGGCTGGTAGCGTCCAGATTCATAACTTTGAGGTGTTGCCTTGTCATTTTTTGGTGTAA	PEAGSVQIHNFEVLPCHFLV*	-1.471	0.002142	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25021	CYHSFSLAYTLAPSTLAMQV			20	SLAY-screened peptide P3371			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTACCACTCTTTCTCCCTTGCCTACACCCTGGCGCCTTCCACTCTTGCGATGCAGGTTTAA	CYHSFSLAYTLAPSTLAMQV*	-1.471	0.000573	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25022	FIPINPSVPGLRTTANSSLS			20	SLAY-screened peptide P3372			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCATCCCCATTAATCCTTCCGTGCCTGGTCTCAGGACCACTGCCAATAGCTCTTTGTCGTAA	FIPINPSVPGLRTTANSSLS*	-1.471	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25023	QTPPKNHIIAADIRRIYLNR			20	SLAY-screened peptide P3373			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGACCCCTCCTAAGAACCACATTATTGCCGCTGATATTCGTCGCATCTATCTCAATCGTTAA	QTPPKNHIIAADIRRIYLNR*	-1.471	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25024	RMCFGAD			7	SLAY-screened peptide P3374			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGATGTGTTTTGGGGCTGATTAGGGGGGTCACCTCCTTTGCCTTCGCCACAATATGTATTAA	RMCFGAD*GGHLLCLRHNMY*	-1.47	0.01155	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25025	SPFQQLYDCIRMRQ			14	SLAY-screened peptide P3375			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCCCTTTCAGCAGCTCTACGACTGCATTCGCATGCGGCAGTAGTGGGCCTTGGCTAGTTAA	SPFQQLYDCIRMRQ*WALAS*	-1.47	0.022129	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25026	ANDSRVALTDRLVSQCHNDP			20	SLAY-screened peptide P3376			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAACGACTCGCGCGTCGCTCTGACTGACCGTCTTGTTAGTCAGTGCCACAATGACCCCTAA	ANDSRVALTDRLVSQCHNDP*	-1.47	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25027	CVPNPVGRR			9	SLAY-screened peptide P3377			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGTCCCTAACCCGGTCGGACGACGATAGCAGGTGCTCCGATGGGTCTCCCAATTACGGTTA	CVPNPVGRR*QVLRWVSQLRL	-1.47	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25028	RPRTHINILIAASRHPFVAW			20	SLAY-screened peptide P3378			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCGCGGACTCACATTAACATCCTTATTGCTGCCTCTAGGCATCCGTTTGTGGCCTGGTAA	RPRTHINILIAASRHPFVAW*	-1.469	0.006929	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25029	DSITDHAPPSKDLIIWVYSV			20	SLAY-screened peptide P3379			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTCTATTACCGATCATGCGCCTCCCTCCAAGGACCTGATTATCTGGGTTTACAGCGTTTAA	DSITDHAPPSKDLIIWVYSV*	-1.469	0.000322	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25030	CCPVPHHIVTPYNAIFRCQY			20	SLAY-screened peptide P3380			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTGTCCTGTCCCGCATCATATCGTTACCCCTTATAATGCGATTTTTCGTTGCCAGTACTAA	CCPVPHHIVTPYNAIFRCQY*	-1.469	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25031	CHCTMITTHLTPLLLR			16	SLAY-screened peptide P3381			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCATTGCACCATGATTACTACTCATCTGACGCCCCTTCTTCTGAGGTAGCTGATTACTTAA	CHCTMITTHLTPLLLR*LIT*	-1.468	6.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25032	TPTWAPCLSRIG			12	SLAY-screened peptide P3382			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCTACGTGGGCCCCCTGTTTATCTCGTATAGGTTGAGCAGTGGCCCCCCCATCCGTAACT	TPTWAPCLSRIG*AVAPPSVT	-1.468	0.030469	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25033	YHLNSALTCLSYYRNSTIWA			20	SLAY-screened peptide P3383			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCATCTGAATAGTGCCCTGACTTGTCTGTCGTATTACAGGAATTCCACTATCTGGGCCTAA	YHLNSALTCLSYYRNSTIWA*	-1.467	0.023564	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25034	CVTINTNSSSLGL			13	SLAY-screened peptide P3384			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGTCACGATTAACACTAATTCTAGCTCTTTGGGCTTGTAGAGCCCTAAGAAGGTTACGTAA	CVTINTNSSSLGL*SPKKVT*	-1.467	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25035	SGAQYYPL			8	SLAY-screened peptide P3385			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGGCGCTCAGTACTACCCCCTGTAGGTCTCTGAGGCTGACATGGCTATGTGGTCTGGTTAA	SGAQYYPL*VSEADMAMWSG*	-1.467	0.029426	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25036	RALSTILRLISRIVEAQPTP			20	SLAY-screened peptide P3386			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCGCTGAGCACCATTTTGAGGCTCATCTCCCGGATTGTGGAGGCTCAGCCCACTCCCTAA	RALSTILRLISRIVEAQPTP*	-1.467	0.022574	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25037	LTMNS			5	SLAY-screened peptide P3387			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCACTATGAACTCTTAGCGCTGCAAGGCGTTGCTTCCCGGTACTCATGTACCCAGAAGTAAC	LTMNS*RCKALLPGTHVPRSN	-1.467	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25038	RFLHIMSYLPMLFHILKIIR			20	SLAY-screened peptide P3388			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTTTTTGCACATTATGTCCTACCTTCCTATGCTGTTTCACATCTTGAAGATTATTCGGTAA	RFLHIMSYLPMLFHILKIIR*	-1.467	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25039	LLYPKPTGMPIFSSVHYVSH			20	SLAY-screened peptide P3389			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCTCTATCCCAAGCCCACTGGCATGCCCATTTTTTCTTCCGTGCACTATGTTTCCCATTAA	LLYPKPTGMPIFSSVHYVSH*	-1.466	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25040	NSTPPRSPVRRVCSAYAYLY			20	SLAY-screened peptide P3390			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTCGACGCCGCCCAGGTCTCCTGTGCGCCGTGTTTGTTCCGCCTATGCCTACCTCTACTAA	NSTPPRSPVRRVCSAYAYLY*	-1.466	0.000499	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25041	ISDRTYLNDFLT			12	SLAY-screened peptide P3391			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTCGGACCGGACTTATCTTAATGACTTTCTGACTTAGGATCCCAGCACGCTTTCGATGTAA	ISDRTYLNDFLT*DPSTLSM*	-1.466	0.000429	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25042	SGLYNVIIFFPMGHDDTKAE			20	SLAY-screened peptide P3392			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGGCCTCTATAACGTCATTATCTTCTTTCCGATGGGTCATGACGATACCAAGGCTGAGTAA	SGLYNVIIFFPMGHDDTKAE*	-1.466	0.001331	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25043	YTQNYICGPTLNYLPKDYIH			20	SLAY-screened peptide P3393			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACTCAGAATTATATTTGTGGTCCCACCCTTAATTACCTGCCGAAGGATTATATCCACTAA	YTQNYICGPTLNYLPKDYIH*	-1.466	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25044	HASLVVFRTMTSAALNANHA			20	SLAY-screened peptide P3394			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGCTAGCTTGGTCGTTTTTCGTACGATGACTTCGGCGGCCCTCAATGCCAATCACGCTTAA	HASLVVFRTMTSAALNANHA*	-1.466	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25045	LPSNTMEIMNLGSMSLTYIT			20	SLAY-screened peptide P3395			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCTAGCAATACGATGGAGATCATGAACTTGGGCTCTATGAGCCTCACGTACATTACCTAA	LPSNTMEIMNLGSMSLTYIT*	-1.466	0.001063	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25046	TASY			4	SLAY-screened peptide P3396			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGCCTCTTATTAGCCCCGTTTTACCTCTCCATGATGGGCCGTACGAATAGTCTGACTTAAC	TASY*PRFTSP*WAVRIV*LN	-1.465	0.004676	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25047	TSPISRAHIIRLYHCTFLKA			20	SLAY-screened peptide P3397			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAGTCCCATTTCCCGCGCTCACATTATCCGTCTTTACCATTGTACCTTTTTGAAGGCCTAA	TSPISRAHIIRLYHCTFLKA*	-1.465	0.016094	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25048	QPTSSPTAWGSRAKATARFV			20	SLAY-screened peptide P3398			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCTACCTCTAGTCCTACCGCCTGGGGTTCTCGGGCCAAGGCCACCGCCCGTTTCGTTTAA	QPTSSPTAWGSRAKATARFV*	-1.465	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25049	RFYPLRDASCSNPYAYLVNN			20	SLAY-screened peptide P3399			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTTTTATCCCTTGCGTGATGCTTCTTGTAGCAACCCCTACGCTTACCTCGTTAATAACTAA	RFYPLRDASCSNPYAYLVNN*	-1.465	0.026879	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25050	LVFFSFVFVRFFRVERTNHA			20	SLAY-screened peptide P3400			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGTTTTTTTCTCCTTCGTGTTCGTTCGGTTTTTCCGTGTTGAGCGCACCAATCATGCGTAA	LVFFSFVFVRFFRVERTNHA*	-1.465	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25051	APAEHEVQHDPHHDSSITSA			20	SLAY-screened peptide P3401			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCCGCTGAGCATGAGGTTCAGCATGACCCCCATCATGATAGTAGCATCACGAGTGCTTAA	APAEHEVQHDPHHDSSITSA*	-1.465	0.01766	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25052	FNLNWHGPL			9	SLAY-screened peptide P3402			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTAACCTTAACTGGCATGGCCCCTTGTAGGTTATCAAGAGCCCCACCTTTGTTCTTGTGTAA	FNLNWHGPL*VIKSPTFVLV*	-1.465	0.030987	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25053	LMGYTNFSAHVQYN			14	SLAY-screened peptide P3403			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCATGGGGTATACCAATTTTAGTGCCCATGTTCAGTACAACTAGTAGAACGATTGCACCTAA	LMGYTNFSAHVQYN**NDCT*	-1.465	0.003191	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25054	WCSADPRAD			9	SLAY-screened peptide P3404			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTGCTCGGCAGACCCACGAGCCGACTAGCCGGTTGCATAAGTTCCATTCCACCCTGTAACT	WCSADPRAD*PVA*VPFHPVT	-1.465	6.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25055	ARPPYWLESSVHGLRLTHLP			20	SLAY-screened peptide P3405			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGAGGCCGCCCTACTGGCTGGAGTCGAGTGTGCACGGCTTGCGCCTTACTCATCTGCCGTAA	ARPPYWLESSVHGLRLTHLP*	-1.465	0.029622	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25056	TSSSLMRILSIIWLLAIMRCN			21	SLAY-screened peptide P3406			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAGCAGCAGTCTGATGCGTATCCTCTCTATCATCTGGCTCCTTGCCATCATGCGCTGTAAC	TSSSLMRILSIIWLLAIMRCN	-1.465	0.008573	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25057	SQRGLANPSHSVNACNDGVTE			21	SLAY-screened peptide P3407			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCAGCGCGGTCTGGCCAACCCGTCCCATTCCGTTAATGCGTGTAACGATGGAGTAACTGAG	SQRGLANPSHSVNACNDGVTE	-1.464	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25058	YCSPGFYGVSPRMYIHAINS			20	SLAY-screened peptide P3408			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTGTAGTCCTGGCTTCTATGGCGTCAGCCCCAGGATGTACATTCATGCGATTAATAGTTAA	YCSPGFYGVSPRMYIHAINS*	-1.464	0.034463	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25059	PSSCIELRVYLIIRLKICRY			20	SLAY-screened peptide P3409			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTCGTCGTGTATCGAGCTGCGCGTCTACCTCATCATTCGCCTTAAGATCTGCCGTTATTAA	PSSCIELRVYLIIRLKICRY*	-1.464	0.011426	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25060	VTADSSMLKGDFESLTIILL			20	SLAY-screened peptide P3410			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCACTGCTGACAGCAGCATGCTGAAGGGCGATTTCGAGAGTCTTACTATCATCTTGCTTTAA	VTADSSMLKGDFESLTIILL*	-1.464	0.045581	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25061	PILDNFHRANTVRVYGLNDI			20	SLAY-screened peptide P3411			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATCCTGGATAATTTCCACCGTGCTAATACTGTCCGCGTGTACGGCCTTAACGACATCTAA	PILDNFHRANTVRVYGLNDI*	-1.463	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25062	VPYHAILSALVNRLLRRLVH			20	SLAY-screened peptide P3412			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCCCTACCACGCCATTCTCTCCGCTCTCGTGAATCGCCTTCTTCGCCGGCTCGTCCACTAA	VPYHAILSALVNRLLRRLVH*	-1.463	6.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25063	SSVPDLDDNPLLHSPISLSK			20	SLAY-screened peptide P3413			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGAGCGTGCCTGACTTGGATGATAATCCCCTTCTGCATTCGCCCATTAGCCTTTCCAAGTAA	SSVPDLDDNPLLHSPISLSK*	-1.463	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25064	LRLGCISGAFVVPYPTTDNM			20	SLAY-screened peptide P3414			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAGGCTCGGCTGTATCTCTGGTGCCTTTGTTGTCCCGTACCCTACTACTGACAACATGTAA	LRLGCISGAFVVPYPTTDNM*	-1.463	0.006517	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25065	PRRSPQVLEDSRR			13	SLAY-screened peptide P3415			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGCCGTAGCCCCCAGGTCCTTGAGGATTCGCGTCGCTAGTGGGCCTTTGGCATCACTTAA	PRRSPQVLEDSRR*WAFGIT*	-1.462	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25066	TSINEFNKWFTVKSNTITNL			20	SLAY-screened peptide P3416			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTCCATTAATGAGTTTAATAAGTGGTTCACCGTCAAGTCTAACACCATCACGAACCTTTAA	TSINEFNKWFTVKSNTITNL*	-1.462	0.015546	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25067	FSDVLWRPPTWVSHSDPTFF			20	SLAY-screened peptide P3417			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTCGGATGTTTTGTGGCGCCCCCCTACCTGGGTCTCTCATAGCGATCCGACTTTCTTTTAA	FSDVLWRPPTWVSHSDPTFF*	-1.462	0.008772	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25068	PTWQDWITTLQYEYHSFPNL			20	SLAY-screened peptide P3418			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACTTGGCAGGATTGGATCACCACCCTCCAGTATGAGTACCACTCCTTCCCTAATCTCTAA	PTWQDWITTLQYEYHSFPNL*	-1.461	2.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25069	SPPSSLTTCFIVALSCVRLPN			21	SLAY-screened peptide P3419			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCCGCCCTCGTCTTTGACAACCTGCTTCATCGTGGCACTCTCCTGCGTCCGACTGCCTAAC	SPPSSLTTCFIVALSCVRLPN	-1.461	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25070	PLPQGGDPSYKCRFNYFNYA			20	SLAY-screened peptide P3420			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTTCCCCAGGGCGGCGACCCCAGCTACAAGTGCCGCTTTAACTACTTTAATTACGCTTAA	PLPQGGDPSYKCRFNYFNYA*	-1.461	0.00011	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25071	RLSLYLEPVPVYAL			14	SLAY-screened peptide P3421			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCTTTCGCTTTACCTTGAGCCGGTCCCTGTCTATGCCCTCTAGGACACCTCGCTTCTGTAA	RLSLYLEPVPVYAL*DTSLL*	-1.461	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25072	FIPLIYYITSLH			12	SLAY-screened peptide P3422			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTATCCCGTTGATCTATTACATTACGAGTCTTCACTAGGCCTTCTCCGAGACTACTAACTAA	FIPLIYYITSLH*AFSETTN*	-1.46	0.000389	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25073	HHSMDQIPPEAGQDPFDTNW			20	SLAY-screened peptide P3423			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCACTCGATGGATCAGATTCCTCCCGAGGCCGGCCAGGATCCGTTCGACACTAACTGGTAA	HHSMDQIPPEAGQDPFDTNW*	-1.46	0.002168	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25074	LHRFSKNRASLLCIIGLFPF			20	SLAY-screened peptide P3424			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCATCGCTTTTCTAAGAACCGCGCCAGTTTGTTGTGTATTATTGGTCTCTTTCCTTTTTAA	LHRFSKNRASLLCIIGLFPF*	-1.46	0.017387	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25075	DY			2	SLAY-screened peptide P3425			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTATTAGAGTTCGCGGTACTATATGAACCTCAACCCCATTTTCATTGTGTACTCTACTTAA	DY*SSRYYMNLNPIFIVYST*	-1.46	6.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25076	VYPVLPWILYILVVSLL			17	SLAY-screened peptide P3426			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTATCCGGTGCTCCCGTGGATTCTCTATATTCTTGTTGTGAGCCTTCTTTAGGGGGATTAA	VYPVLPWILYILVVSLL*GD*	-1.46	0.00016	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25077	CDVATVIAAPLTVLRLTMVTN			21	SLAY-screened peptide P3427			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGACGTGGCAACTGTAATCGCAGCACCGCTTACCGTACTACGCCTCACCATGGTCACTAAC	CDVATVIAAPLTVLRLTMVTN	-1.46	6.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25078	QLPKIIRPNNIRAGARCITC			20	SLAY-screened peptide P3428			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTTGCCCAAGATTATTAGGCCCAACAATATCCGTGCGGGGGCTAGGTGCATTACGTGTTAA	QLPKIIRPNNIRAGARCITC*	-1.459	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25079	HHTTPIPDVSSSVMPINARY			20	SLAY-screened peptide P3429			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCATACTACCCCTATTCCTGACGTTTCTAGCAGTGTCATGCCCATCAATGCCCGGTACTAA	HHTTPIPDVSSSVMPINARY*	-1.459	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25080	QRHSAPLAFASGPWPVIPTGN			21	SLAY-screened peptide P3430			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCGTCATTCCGCTCCACTTGCGTTCGCATCTGGACCATGGCCGGTCATACCTACAGGTAAC	QRHSAPLAFASGPWPVIPTGN	-1.459	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25081	PSKRRFVLRLVLCVHSSRFD			20	SLAY-screened peptide P3431			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGCAAGCGGAGGTTCGTTCTCCGCCTTGTTCTCTGTGTGCACTCTTCGAGGTTTGACTAA	PSKRRFVLRLVLCVHSSRFD*	-1.459	0.017145	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25082	FDRWHGNIHSNYFNTMLPLY			20	SLAY-screened peptide P3432			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGACCGTTGGCATGGCAACATTCACTCTAACTACTTTAACACGATGCTTCCTCTCTACTAA	FDRWHGNIHSNYFNTMLPLY*	-1.459	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25083	GRTSAWSMATRLGHVYNSAT			20	SLAY-screened peptide P3433			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCGCACCAGCGCTTGGAGTATGGCCACTCGTCTCGGCCATGTTTATAATTCGGCTACTTAA	GRTSAWSMATRLGHVYNSAT*	-1.459	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25084	IDGCRSLSNRCRTRH			15	SLAY-screened peptide P3434			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGATGGCTGTAGGAGCCTTAGTAACCGCTGTAGGACCCGCCATTAGAACAGTAGGTCCTAA	IDGCRSLSNRCRTRH*NSRS*	-1.458	0.000946	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25085	LFPFPYRCYYRDFADNSWVN			20	SLAY-screened peptide P3435			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTCCCCTTCCCTTATCGCTGCTATTATCGTGATTTTGCCGATAATAGCTGGGTCAATTAA	LFPFPYRCYYRDFADNSWVN*	-1.458	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25086	CGVNIPLMLKCHDYRLPTPD			20	SLAY-screened peptide P3436			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGGTGTGAATATCCCGCTTATGCTTAAGTGTCATGATTACCGCCTGCCTACCCCTGATTAA	CGVNIPLMLKCHDYRLPTPD*	-1.458	0.007227	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25087	TDFLMVNNGIRGAA			14	SLAY-screened peptide P3437			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGATTTTCTCATGGTCAATAACGGCATTAGGGGGGCCGCCTGACTGACCACTGGCTCTAAC	TDFLMVNNGIRGAA*LTTGSN	-1.458	0.021615	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25088	FSRRLFNRFLFLFALRGYGN			20	SLAY-screened peptide P3438			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAGCCGCAGGCTGTTTAATCGGTTTCTGTTCCTTTTTGCCCTCCGGGGGTACGGTAACTAA	FSRRLFNRFLFLFALRGYGN*	-1.457	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25089	YSSPIFILHLDDKWSTPPLS			20	SLAY-screened peptide P3439			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTCGTCCCCTATTTTTATCCTGCACCTCGATGATAAGTGGAGTACCCCGCCCCTGAGCTAA	YSSPIFILHLDDKWSTPPLS*	-1.457	0.003288	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25090	DHPHVGRPSSILKICCYEEE			20	SLAY-screened peptide P3440			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCATCCCCATGTTGGTCGTCCTTCCAGCATCTTGAAGATCTGCTGTTACGAGGAGGAGTAA	DHPHVGRPSSILKICCYEEE*	-1.457	0.000102	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25091	ISFIIGNCVRCDYHVMSSAL			20	SLAY-screened peptide P3441			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAGCTTCATCATTGGGAACTGTGTTCGCTGCGATTACCATGTCATGTCGAGTGCCCTTTAA	ISFIIGNCVRCDYHVMSSAL*	-1.456	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25092	GSYTFSHRSNPTTFCLALHT			20	SLAY-screened peptide P3442			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTCCTACACCTTTTCCCATCGCTCGAATCCGACCACGTTTTGTCTCGCGCTGCACACCTAA	GSYTFSHRSNPTTFCLALHT*	-1.456	0.028999	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25093	ASGLRHPRPRAPTTFTPDSK			20	SLAY-screened peptide P3443			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTCTGGTCTTCGTCACCCTCGGCCCAGGGCCCCTACTACGTTCACTCCTGATTCTAAGTAA	ASGLRHPRPRAPTTFTPDSK*	-1.456	0.021618	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25094	SFPATCRLLARVCSAPLFITN			21	SLAY-screened peptide P3444			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTTCCCTGCTACGTGTCGACTTCTTGCCCGGGTATGCAGTGCACCTCTCTTCATTACTAAC	SFPATCRLLARVCSAPLFITN	-1.456	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25095	GHMINYNTYSIINYRAWCGM			20	SLAY-screened peptide P3445			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCACATGATCAATTACAACACTTATAGCATCATCAATTACCGGGCCTGGTGTGGCATGTAA	GHMINYNTYSIINYRAWCGM*	-1.456	0.000982	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25096	VSHADSTHYGPTDDDCTSTV			20	SLAY-screened peptide P3446			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTCCCACGCGGACTCTACTCACTATGGTCCGACTGACGATGACTGCACGTCCACTGTTTAA	VSHADSTHYGPTDDDCTSTV*	-1.456	0.001934	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25097	CRPFRWIMRRIIPARLRTIN			20	SLAY-screened peptide P3447			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGGCCTTTTAGATGGATCATGCGTCGCATCATACCAGCTCGCCTACGGACTATTAACTGA	CRPFRWIMRRIIPARLRTIN*	-1.456	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25098	SIFRGSAGCELTMPAPDLLQ			20	SLAY-screened peptide P3448			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTATCTTCAGGGGCTCCGCCGGCTGCGAGCTGACTATGCCTGCCCCGGACCTTCTCCAGTAA	SIFRGSAGCELTMPAPDLLQ*	-1.455	0.015422	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25099	SGLTLKGPHHTDDLTSS			17	SLAY-screened peptide P3449			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGGCCTGACCCTGAAGGGCCCCCACCACACCGATGATCTTACTAGTAGTTAGGTCGATTAA	SGLTLKGPHHTDDLTSS*VD*	-1.455	0.041286	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25100	PVKSASVQAVDLVRTEVDRC			20	SLAY-screened peptide P3450			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGTGAAGAGCGCGTCCGTCCAGGCCGTCGATCTGGTCCGCACTGAGGTGGATAGGTGTTAA	PVKSASVQAVDLVRTEVDRC*	-1.455	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25101	PIANEDHRRIAMSMPFAISRN			21	SLAY-screened peptide P3451			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATCGCGAACGAGGACCACCGCAGGATAGCTATGTCAATGCCGTTTGCTATCAGCCGTAAC	PIANEDHRRIAMSMPFAISRN	-1.455	0.031448	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25102	DSVTPSLLTHGIGTRLHTIPN			21	SLAY-screened peptide P3452			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTCGGTGACCCCCTCTCTCTTGACGCACGGTATTGGTACTCGGCTTCACACAATTCCTAAC	DSVTPSLLTHGIGTRLHTIPN	-1.455	0.00818	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25103	GSLPGHVRNTRIIATGRNRN			20	SLAY-screened peptide P3453			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGTCTTTGCCCGGTCACGTGCGCAATACTAGGATCATTGCCACTGGCCGTAACCGCAATTAA	GSLPGHVRNTRIIATGRNRN*	-1.455	0.000412	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25104	VDPSDTHGSLPNAHIICHLL			20	SLAY-screened peptide P3454			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGATCCTAGTGACACCCATGGCTCCCTCCCCAACGCTCACATTATCTGCCACTTGCTTTAA	VDPSDTHGSLPNAHIICHLL*	-1.455	0.000389	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25105	LHSLCIPHWYANAYSNLHLV			20	SLAY-screened peptide P3455			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCACAGTTTGTGCATCCCCCACTGGTACGCGAATGCTTATAGTAATCTGCACCTCGTCTAA	LHSLCIPHWYANAYSNLHLV*	-1.454	2.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25106	PNNKMCYVFHSLNRHHIGLS			20	SLAY-screened peptide P3456			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAATAACAAGATGTGCTATGTCTTCCATAGTCTTAACCGCCATCATATCGGGCTCAGCTAA	PNNKMCYVFHSLNRHHIGLS*	-1.454	0.000633	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25107	DIHTLILLLVPCPLRRSTLLN			21	SLAY-screened peptide P3457			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACATTCATACTCTCATCTTACTGCTAGTACCCTGCCCTTTGCGGCGCTCAACATTGCTTAAC	DIHTLILLLVPCPLRRSTLLN	-1.454	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25108	PDFVTHCRVYRHCCKRSILS			20	SLAY-screened peptide P3458			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGACTTCGTGACTCACTGTCGCGTCTATCGCCATTGTTGTAAGCGGTCCATCCTCTCTTAA	PDFVTHCRVYRHCCKRSILS*	-1.454	0.011255	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25109	LTQTLPEPDPRDTR			14	SLAY-screened peptide P3459			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGACTCAGACGCTCCCGGAGCCCGACCCTCGGGACACCCGGTAGGGCTCGAGTGGTTTGTAA	LTQTLPEPDPRDTR*GSSGL*	-1.454	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25110	QAGVCCTWRPPLPLPSPDRSL			21	SLAY-screened peptide P3460			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGCCGGGGTCTGCTGCACCTGGCGGCCCCCGCTCCCTCTACCTTCTCCGGACCGGTCATTA	QAGVCCTWRPPLPLPSPDRSL	-1.454	0.030205	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25111	SLYHSQPCSGTISISVYYTP			20	SLAY-screened peptide P3461			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCTTTATCATAGTCAGCCTTGTAGTGGTACGATTAGTATTTCCGTCTATTACACCCCTTAA	SLYHSQPCSGTISISVYYTP*	-1.454	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25112	IVMLGISMAPNVPKKSNISI			20	SLAY-screened peptide P3462			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGTGATGCTTGGGATTTCGATGGCTCCGAACGTCCCTAAGAAGTCTAATATCTCTATTTAA	IVMLGISMAPNVPKKSNISI*	-1.454	0.012451	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25113	SSPIVPRDGFCKNKADANNH			20	SLAY-screened peptide P3463			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAGCCCGATTGTTCCCAGGGATGGGTTCTGCAAGAATAAGGCGGATGCTAATAACCATTAA	SSPIVPRDGFCKNKADANNH*	-1.453	0.002669	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25114	HVPRNSMSLALNLLVSYCTY			20	SLAY-screened peptide P3464			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGTCCCTCGTAACTCTATGTCCCTCGCTCTTAACCTGCTCGTGTCTTATTGTACGTATTAA	HVPRNSMSLALNLLVSYCTY*	-1.453	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25115	RSCTNLTY			8	SLAY-screened peptide P3465			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAGCTGCACTAATCTTACCTATTAGGCGCAGAACTGTTCGAATAGGTTGTGTATTTAGTAA	RSCTNLTY*AQNCSNRLCI**	-1.453	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25116	SPVGMC			6	SLAY-screened peptide P3466			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCCGGTTGGGATGTGCTAGGCGTAGCGTTTCGGGGATCGCACTTGGTATACGCACCTGTAA	SPVGMC*A*RFGDRTWYTHL*	-1.453	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25117	LGTLGFAYYCSCTFHFSLYR			20	SLAY-screened peptide P3467			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGGCACCCTTGGCTTTGCTTATTATTGTTCCTGCACCTTCCATTTCTCTCTCTACAGGTAA	LGTLGFAYYCSCTFHFSLYR*	-1.453	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25118	SPWTQCTLVTFRLYED			16	SLAY-screened peptide P3468			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCCCTGGACTCAGTGTACTTTGGTCACTTTCCGGTTGTACGAGGACTAGAACAGTATTTAA	SPWTQCTLVTFRLYED*NSI*	-1.453	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25119	PAIYTFY			7	SLAY-screened peptide P3469			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCGATCTATACCTTTTATTAGTTCTAGTCCGAGTCCCATGCCCGGGTGGATCGGCTTTAA	PAIYTFY*F*SESHARVDRL*	-1.453	0.001893	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25120	PYMNPVLTAYDGRPSYPLVP			20	SLAY-screened peptide P3470			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTACATGAACCCGGTGCTGACTGCCTATGATGGTCGCCCGAGCTATCCTCTTGTGCCTTAA	PYMNPVLTAYDGRPSYPLVP*	-1.452	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25121	ACIPTLSTRNDLRPPSPNMT			20	SLAY-screened peptide P3471			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTGTATTCCTACTCTGTCCACGCGCAACGATCTTCGTCCGCCTTCGCCTAATATGACGTAA	ACIPTLSTRNDLRPPSPNMT*	-1.452	0.005856	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25122	TVFYPPCHFDRCLARRPFAR			20	SLAY-screened peptide P3472			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTCTTCTACCCCCCCTGCCATTTTGATCGGTGTTTGGCTCGTAGGCCGTTCGCCCGCTAA	TVFYPPCHFDRCLARRPFAR*	-1.452	0.03662	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25123	RSRSY			5	SLAY-screened peptide P3473			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTCTCGTTCCTATTAGCCCATTTGGGGGCCGTGGTATTTGAATGAGCACGCCTCCTGTTAA	RSRSY*PIWGPWYLNEHASC*	-1.452	9.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25124	LIAHCTNSGNFPKHAM			16	SLAY-screened peptide P3474			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGATTGCCCATTGCACTAATTCTGGTAATTTTCCTAAGCATGCTATGTAGCATTCTTGCTAA	LIAHCTNSGNFPKHAM*HSC*	-1.452	3.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25125	CFPDCWMR			8	SLAY-screened peptide P3475			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTTCCCCGACTGCTGGATGCGGTAGGCGCTCAAGCTGGTTGTTCAGTACTTGGACTGTTAA	CFPDCWMR*ALKLVVQYLDC*	-1.451	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25126	LRDLNCTLPYFLIRVSPDVL			20	SLAY-screened peptide P3476			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCGCGACCTTAATTGTACGCTTCCTTACTTTCTGATTCGGGTGTCGCCCGACGTGCTGTAA	LRDLNCTLPYFLIRVSPDVL*	-1.451	0.026949	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25127	PSRCPSHRFNCAPSLNMLST			20	SLAY-screened peptide P3477			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTCCCGGTGTCCCTCTCACCGTTTTAATTGCGCGCCCTCCCTTAATATGTTGTCCACCTAA	PSRCPSHRFNCAPSLNMLST*	-1.451	0.004147	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25128	RRPDALGPSYSVPRFTLIRL			20	SLAY-screened peptide P3478			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGCCCGGATGCTCTTGGGCCCAGCTACTCCGTTCCTAGGTTCACCCTCATTCGTCTGTAA	RRPDALGPSYSVPRFTLIRL*	-1.449	0.002503	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25129	PRLWLLHCILYITSRIRLRHN			21	SLAY-screened peptide P3479			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGTCTGTGGCTTTTGCACTGTATCCTCTATATTACTAGTCGTATTAGGCTCAGGCATAAC	PRLWLLHCILYITSRIRLRHN	-1.449	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25130	TSFANSHRPLYILPMICTASN			21	SLAY-screened peptide P3480			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCCTTTGCTAACTCGCACAGGCCCCTCTACATTTTGCCCATGATCTGTACGGCTTCTAAC	TSFANSHRPLYILPMICTASN	-1.449	0.028075	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25131	SGARVSFLYW			10	SLAY-screened peptide P3481			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGGGGCCCGCGTCTCTTTTCTGTATTGGTAGTTGTGCTTCCTCCTCTCTGACCATTGCTAA	SGARVSFLYW*LCFLLSDHC*	-1.449	0.004686	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25132	NRCSRPFPVPFVDWYMPINL			20	SLAY-screened peptide P3482			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCGTTGCTCGCGCCCGTTCCCGGTGCCCTTTGTCGATTGGTATATGCCTATTAACCTTTAA	NRCSRPFPVPFVDWYMPINL*	-1.449	3.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25133	CKHTHPNHRDHRLTCSSCNQ			20	SLAY-screened peptide P3483			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAAGCACACTCATCCGAATCATCGCGATCACCGCCTGACGTGCAGTAGCTGCAACCAGTAA	CKHTHPNHRDHRLTCSSCNQ*	-1.449	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25134	VHPFHFKNHIDMHTFVPHVN			20	SLAY-screened peptide P3484			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCATCCTTTCCACTTCAAGAACCACATCGATATGCACACTTTTGTCCCGCATGTGAATTAA	VHPFHFKNHIDMHTFVPHVN*	-1.448	0.014845	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25135	HYFLLPRPMTLWRTFLYAKG			20	SLAY-screened peptide P3485			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTATTTCCTGTTGCCGAGGCCCATGACCTTGTGGAGGACGTTTTTGTACGCGAAGGGCTAA	HYFLLPRPMTLWRTFLYAKG*	-1.448	0.002896	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25136	PLWPCSQYPNCNDVLSPGGY			20	SLAY-screened peptide P3486			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCTGGCCCTGTTCTCAGTATCCTAATTGTAACGATGTCCTCAGCCCCGGGGGCTACTAA	PLWPCSQYPNCNDVLSPGGY*	-1.448	0.00609	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25137	PMALSAWH			8	SLAY-screened peptide P3487			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATGGCCTTGAGCGCTTGGCACTAGAATGCCCCCATTGCTATGCAGATTAGTCCCATTTAA	PMALSAWH*NAPIAMQISPI*	-1.448	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25138	LCPSSAL			7	SLAY-screened peptide P3488			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTGTCCTTCGAGCGCCTTGTAGTCTCGCCCCCACCCCTACCATCGCTCCCGCGACGGTTAA	LCPSSAL*SRPHPYHRSRDG*	-1.447	0.000279	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25139	PPVHVCSLLF			10	SLAY-screened peptide P3489			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCCGTTCACGTTTGCTCTCTCCTGTTTTAGCATATCATGTTTTACGATACGCCCGATTAA	PPVHVCSLLF*HIMFYDTPD*	-1.447	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25140	LSGCLSTTYWHCYCPIFNSI			20	SLAY-screened peptide P3490			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCTGGTTGTCTTAGTACTACCTACTGGCATTGTTATTGTCCTATTTTTAATAGTATTTAA	LSGCLSTTYWHCYCPIFNSI*	-1.447	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25141	DTVIPRFSWCPSLLYYLPTY			20	SLAY-screened peptide P3491			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACACCGTTATTCCGCGGTTCTCCTGGTGTCCGTCTCTTCTTTACTATCTGCCGACGTACTAA	DTVIPRFSWCPSLLYYLPTY*	-1.446	2.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25142	YRALNCDRRNRLYNSKKEVS			20	SLAY-screened peptide P3492			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCGCGCCCTTAACTGCGACCGGCGCAACCGCTTGTACAATTCGAAGAAGGAGGTCTCGTAA	YRALNCDRRNRLYNSKKEVS*	-1.446	0.014657	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25143	LVKRCSQRSYHLVYPPHTTP			20	SLAY-screened peptide P3493			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGTTAAGCGCTGCTCTCAGCGCAGCTACCACTTGGTTTACCCCCCGCATACCACGCCCTAA	LVKRCSQRSYHLVYPPHTTP*	-1.446	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25144	MSLTSACVWAHDDQGRDSFL			20	SLAY-screened peptide P3494			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTCCCTGACGTCCGCCTGTGTGTGGGCGCATGACGATCAGGGGCGGGATTCCTTTCTTTAA	MSLTSACVWAHDDQGRDSFL*	-1.446	0.000416	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25145	VGSAICARARRTAVPHAVSHL			21	SLAY-screened peptide P3495			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGGCTCCGCTATCTGCGCTCGCGCGCGCAGGACTGCGGTTCCTCATGCTGTCTCTCACTTA	VGSAICARARRTAVPHAVSHL	-1.446	0.000132	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25146	YSDYNNCPTSDHILVRYGTN			20	SLAY-screened peptide P3496			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTCTGACTACAACAACTGTCCTACCTCGGACCACATCCTGGTCCGCTACGGCACCAATTAA	YSDYNNCPTSDHILVRYGTN*	-1.446	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25147	TRNSTCTGRYILL			13	SLAY-screened peptide P3497			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCGTAACAGCACCTGTACCGGCCGGTATATTCTTTTGTAGAGACTGACGCTACGCCGTAAC	TRNSTCTGRYILL*RLTLRRN	-1.446	0.00062	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25148	RPVPHPNGHCFSTCLITQAH			20	SLAY-screened peptide P3498			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCTGTCCCTCATCCTAATGGCCACTGTTTTTCCACTTGCCTGATTACCCAGGCCCACTAA	RPVPHPNGHCFSTCLITQAH*	-1.446	0.010901	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25149	TALSPVSCPILYVTAYNHP			19	SLAY-screened peptide P3499			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGCTCTCAGCCCCGTCTCCTGCCCTATCCTTTATGTTACCGCCTACAATCATCCGTAACTG	TALSPVSCPILYVTAYNHP*L	-1.445	0.002782	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25150	PGIEPTDVIPSHSHVSFYTR			20	SLAY-screened peptide P3500			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGTATCGAGCCCACGGACGTGATCCCCAGTCACAGTCATGTCAGTTTTTACACCAGGTAA	PGIEPTDVIPSHSHVSFYTR*	-1.445	0.003898	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25151	NFNDRFIHECCSYCHWLGSW			20	SLAY-screened peptide P3501			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTTCAATGATAGGTTTATTCACGAGTGCTGCAGTTATTGTCACTGGTTGGGTAGTTGGTAA	NFNDRFIHECCSYCHWLGSW*	-1.445	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25152	TFISHMA			7	SLAY-screened peptide P3502			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTTTATTAGTCACATGGCCTAGGCCCCGCCGAAGAGCTCGTAGAATCATGTCGATAATTAA	TFISHMA*APPKSS*NHVDN*	-1.445	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25153	HINTSRFEIAWCSLRNPSVS			20	SLAY-screened peptide P3503			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACATTAACACTTCTCGCTTCGAGATCGCTTGGTGTTCTCTTCGTAACCCGAGCGTCTCCTAA	HINTSRFEIAWCSLRNPSVS*	-1.445	0.000197	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25154	FRIAGTRPTWTTLPSN			16	SLAY-screened peptide P3504			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCGTATTGCTGGGACCCGGCCAACGTGGACTACTTTACCGTCTAACTGAGTAAGTCGACCT	FRIAGTRPTWTTLPSN*VSRP	-1.444	0.001089	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25155	RAVADALLPPAHTSVYVSSV			20	SLAY-screened peptide P3505			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCGGTGGCGGATGCTCTCCTGCCCCCTGCTCACACTTCTGTGTACGTCTCTTCCGTTTAA	RAVADALLPPAHTSVYVSSV*	-1.444	0.005424	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25156	AC			2	SLAY-screened peptide P3506			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTGTTAGAAGCCCTCGCCCCCCGGCCCCTTGCCTAGTTAGCCCCCCTTCTAGCCGCCTTAA	AC*KPSPPGPLPS*PPF*PP*	-1.443	0.018603	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25157	CPLPLFRTLPMYSVPRISLT			20	SLAY-screened peptide P3507			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCGCTTCCGCTTTTTCGGACCCTGCCTATGTACTCCGTGCCTCGGATCTCGCTTACGTAA	CPLPLFRTLPMYSVPRISLT*	-1.443	0.000531	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25158	PYRAPCPYFYISALRSGSPN			20	SLAY-screened peptide P3508			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTACAGGGCGCCGTGCCCCTATTTTTACATTTCTGCTTTGCGTAGTGGTTCGCCTAATTAA	PYRAPCPYFYISALRSGSPN*	-1.443	0.007957	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25159	CRFITPTYTLVYNDSDNYDI			20	SLAY-screened peptide P3509			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCGTTTTATTACCCCTACTTACACTCTGGTGTATAATGACAGTGACAACTACGATATTTAA	CRFITPTYTLVYNDSDNYDI*	-1.443	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25160	AVNPPPSELDCIIIPLPPRA			20	SLAY-screened peptide P3510			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGTCAACCCTCCTCCTTCGGAGCTCGATTGTATCATCATCCCGCTGCCGCCGCGCGCTTAA	AVNPPPSELDCIIIPLPPRA*	-1.443	0.003315	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25161	VTNLLSDTDRINMRHVHVLH			20	SLAY-screened peptide P3511			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGACTAACCTCCTGTCGGATACTGATCGTATTAATATGCGTCACGTGCACGTCCTCCATTAA	VTNLLSDTDRINMRHVHVLH*	-1.443	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25162	SVYPSPSPHELPSFNTLLAP			20	SLAY-screened peptide P3512			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGTTTATCCTTCCCCGTCCCCTCACGAGTTGCCTAGCTTTAATACTTTGCTGGCCCCTTAA	SVYPSPSPHELPSFNTLLAP*	-1.443	0.000226	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25163	SRYLVSPFFMRHRATLSPNA			20	SLAY-screened peptide P3513			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCAGGTACCTGGTCAGCCCCTTTTTTATGAGGCACCGCGCCACTCTTTCTCCGAACGCTTAA	SRYLVSPFFMRHRATLSPNA*	-1.442	0.000793	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25164	LPATRSLMFVNLFFYSIHNF			20	SLAY-screened peptide P3514			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCCGCGACGCGCAGCCTCATGTTCGTTAACCTCTTTTTTTACTCTATCCATAATTTCTAA	LPATRSLMFVNLFFYSIHNF*	-1.442	0.008982	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25165	ALPCPWSTISCNKAYHPSFI			20	SLAY-screened peptide P3515			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTTGCCCTGTCCCTGGAGTACGATTTCGTGCAATAAGGCCTACCATCCCTCTTTTATTTAA	ALPCPWSTISCNKAYHPSFI*	-1.442	0.000496	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25166	LIRDAPRKIASWISDFLCSP			20	SLAY-screened peptide P3516			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGATTCGCGACGCGCCCCGGAAGATCGCTTCGTGGATCTCGGATTTCCTCTGTTCTCCGTAA	LIRDAPRKIASWISDFLCSP*	-1.441	0.021602	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25167	VGRLTDS			7	SLAY-screened peptide P3517			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGGGTCGTCTGACGGACTCTTAGCCCCACCAGGGTCGTGGCTACAAGAACCCCTTTTATTAA	VGRLTDS*PHQGRGYKNPFY*	-1.441	0.001654	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25168	SSSHVGLSVAPSNNTNDGQS			20	SLAY-screened peptide P3518			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTCGAGTCACGTTGGCCTCTCTGTTGCCCCCTCTAACAACACGAATGACGGCCAGTCCTAA	SSSHVGLSVAPSNNTNDGQS*	-1.441	6.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25169	TLLNVISNVLTSLLFICALFN			21	SLAY-screened peptide P3519			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTCCTGAACGTCATCTCGAATGTGTTAACGTCCCTGCTATTTATCTGTGCTCTCTTTAAC	TLLNVISNVLTSLLFICALFN	-1.441	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25170	GVPLLPPLCCLIPNCLPAFI			20	SLAY-screened peptide P3520			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGGTGCCTTTGTTGCCCCCCCTGTGCTGTTTGATTCCCAATTGTCTGCCGGCCTTCATTTAA	GVPLLPPLCCLIPNCLPAFI*	-1.441	0.000165	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25171	PLSTALYVLYAYSVSLSYSM			20	SLAY-screened peptide P3521			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTTCCACTGCCCTCTATGTGCTTTATGCGTATTCCGTTTCTCTCAGCTATAGCATGTAA	PLSTALYVLYAYSVSLSYSM*	-1.441	0.000268	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25172	LPVDSSPPFVSCLYYHNYC			19	SLAY-screened peptide P3522			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCTGTCGATTCTTCTCCTCCCTTTGTTTCTTGTCTTTATTACCACAATTACTGTTAGTAC	LPVDSSPPFVSCLYYHNYC*Y	-1.441	0.014094	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25173	NASPNGANVRMPTLVLWSHR			20	SLAY-screened peptide P3523			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGCTAGCCCCAACGGCGCCAACGTTAGGATGCCCACCCTTGTCCTTTGGTCGCACCGCTAA	NASPNGANVRMPTLVLWSHR*	-1.44	0.00011	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25174	FTKCSVVH			8	SLAY-screened peptide P3524			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCACCAAGTGTTCGGTCGTTCATTAGCTGCATAACCCCGACCCCGCGGTGACTTATCTTTAA	FTKCSVVH*LHNPDPAVTYL*	-1.44	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25175	AKGPCRTIPIACVILRLIMI			20	SLAY-screened peptide P3525			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGAAGGGCCCGTGCCGCACTATCCCTATTGCTTGTGTGATCCTGAGGCTTATTATGATTTAA	AKGPCRTIPIACVILRLIMI*	-1.439	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25176	RGPRTLPIPITDIELFTACL			20	SLAY-screened peptide P3526			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGGCCCTCGTACGTTGCCCATCCCGATTACGGATATCGAGCTCTTTACTGCCTGCTTGTAA	RGPRTLPIPITDIELFTACL*	-1.438	0.000106	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25177	DHQTHYCTRNCTFVKQVLSY			20	SLAY-screened peptide P3527			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCATCAGACTCATTATTGTACTCGCAATTGCACGTTCGTTAAGCAGGTCCTCTCTTATTAA	DHQTHYCTRNCTFVKQVLSY*	-1.438	0.014941	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25178	YCPPTPHPCRLFPVPNTYAH			20	SLAY-screened peptide P3528			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTGTCCTCCCACCCCTCACCCGTGCCGCCTCTTCCCCGTCCCCAATACTTACGCGCACTAA	YCPPTPHPCRLFPVPNTYAH*	-1.438	0.035396	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25179	PTYDIISMGGHTNTDCIHDG			20	SLAY-screened peptide P3529			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACTTATGATATCATCAGCATGGGCGGTCACACGAATACGGACTGTATCCACGACGGGTAA	PTYDIISMGGHTNTDCIHDG*	-1.438	0.000149	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25180	AQNNHRMPRPERRDRMGLSC			20	SLAY-screened peptide P3530			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCAGAATAATCACCGGATGCCCCGCCCCGAGCGGCGGGACCGGATGGGTTTGAGTTGTTAA	AQNNHRMPRPERRDRMGLSC*	-1.438	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25181	HKSPTDPVQNTPTRFTTDCP			20	SLAY-screened peptide P3531			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAAGAGCCCCACTGATCCCGTCCAGAACACGCCGACTCGCTTTACGACCGATTGTCCTTAA	HKSPTDPVQNTPTRFTTDCP*	-1.437	7.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25182	DALQQDHALGHNAE			14	SLAY-screened peptide P3532			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGCTCTCCAGCAGGATCATGCCCTCGGGCATAACGCCGAGTAGGTGTACTGGGCCAGCTAA	DALQQDHALGHNAE*VYWAS*	-1.437	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25183	SRRIAFDHPDRN			12	SLAY-screened peptide P3533			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCGCAGGATTGCCTTCGACCACCCTGATCGTAATTAGTTCCAGGCTTCTTATCGCGTCTAA	SRRIAFDHPDRN*FQASYRV*	-1.436	0.010853	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25184	IKQSHMALYISTDRRASCQW			20	SLAY-screened peptide P3534			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAAGCAGTCGCATATGGCTCTCTACATCAGCACTGACAGGAGGGCTAGTTGCCAGTGGTAA	IKQSHMALYISTDRRASCQW*	-1.436	0.00157	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25185	FPGGSPQTGGSHSVTGQLHR			20	SLAY-screened peptide P3535			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCCCGGTGGCAGCCCGCAGACGGGGGGTTCCCATTCCGTTACTGGGCAGTTGCACCGTTAA	FPGGSPQTGGSHSVTGQLHR*	-1.436	0.004324	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25186	RHEHFDFPCVDYLIYLTDFA			20	SLAY-screened peptide P3536			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCATGAGCACTTTGATTTTCCGTGTGTTGACTATTTGATCTACCTGACGGATTTTGCCTAA	RHEHFDFPCVDYLIYLTDFA*	-1.436	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25187	GMPGAPLYWNVKIIPLIWAY			20	SLAY-screened peptide P3537			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCATGCCGGGTGCCCCCCTCTATTGGAATGTCAAGATTATCCCTCTCATTTGGGCCTATTAA	GMPGAPLYWNVKIIPLIWAY*	-1.436	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25188	FGYSCSELPLYMDAAMTYTK			20	SLAY-screened peptide P3538			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGGCTATAGCTGTTCTGAGCTCCCGCTCTATATGGACGCCGCCATGACTTACACTAAGTAA	FGYSCSELPLYMDAAMTYTK*	-1.435	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25189	PRSTYYNYHPFSSATIIYSL			20	SLAY-screened peptide P3539			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGTTCGACTTATTATAATTACCATCCTTTTAGCTCGGCGACTATCATTTACTCGCTTTAA	PRSTYYNYHPFSSATIIYSL*	-1.435	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25190	YLEWIYCWSLLGIYPQS			17	SLAY-screened peptide P3540			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCTGGAGTGGATTTATTGCTGGTCGCTTCTCGGTATTTATCCTCAGTCGTAGCCGCATTAA	YLEWIYCWSLLGIYPQS*PH*	-1.435	0.007683	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25191	SHALVLNCTSLLTFAQQWFK			20	SLAY-screened peptide P3541			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCACGCGCTCGTTCTTAACTGTACTTCCCTTCTGACCTTTGCTCAGCAGTGGTTTAAGTAA	SHALVLNCTSLLTFAQQWFK*	-1.435	0.000132	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25192	ARFLRTESCPCKFTNLHLSL			20	SLAY-screened peptide P3542			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCGCTTTTTGCGTACTGAGAGTTGCCCGTGCAAGTTCACTAATTTGCATTTGTCTCTCTAA	ARFLRTESCPCKFTNLHLSL*	-1.435	0.024608	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25193	HMPCRAPYQDSIPSRIETNY			20	SLAY-screened peptide P3543			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACATGCCCTGCCGCGCGCCCTACCAGGATTCGATTCCGAGCCGTATCGAGACGAATTATTAA	HMPCRAPYQDSIPSRIETNY*	-1.435	0.011901	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25194	HPQNRSTNVPRSYNFYSGRT			20	SLAY-screened peptide P3544			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCGCAGAACCGGAGTACTAACGTGCCCAGGTCCTATAATTTTTATTCTGGCAGGACTTAA	HPQNRSTNVPRSYNFYSGRT*	-1.435	0.016621	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25195	RCRPTGLLPRLLPGIFRNHY			20	SLAY-screened peptide P3545			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTGCCGCCCCACTGGTCTCTTGCCCCGTCTGCTGCCTGGTATCTTCCGCAACCATTACTAA	RCRPTGLLPRLLPGIFRNHY*	-1.434	0.000848	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25196	CIYTSASGWRCHRQ			14	SLAY-screened peptide P3546			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCATTTACACTTCTGCTTCGGGCTGGCGTTGCCACCGCCAGTAGCTTTATTATCCCTACTAA	CIYTSASGWRCHRQ*LYYPY*	-1.434	0.010973	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25197	PVNDSLGVPTTHADPTVGFY			20	SLAY-screened peptide P3547			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGTTAACGATTCCCTCGGCGTCCCGACTACGCATGCTGACCCCACTGTTGGCTTCTACTAA	PVNDSLGVPTTHADPTVGFY*	-1.434	0.002812	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25198	PRNKHPGYHFHFRTPILTGV			20	SLAY-screened peptide P3548			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGCAACAAGCACCCGGGTTACCACTTTCATTTTCGTACCCCTATTCTCACCGGCGTTTAA	PRNKHPGYHFHFRTPILTGV*	-1.434	0.006144	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25199	QPLVLPGTPHTSHNVNIFCR			20	SLAY-screened peptide P3549			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCTCTTGTCCTGCCTGGGACGCCTCATACGTCTCACAACGTGAATATCTTCTGCCGCTAA	QPLVLPGTPHTSHNVNIFCR*	-1.434	0.009373	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25200	AWYDFARAVHTNKHVNLLFT			20	SLAY-screened peptide P3550			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTGGTACGACTTTGCGCGTGCCGTCCATACCAACAAGCACGTTAATCTCCTTTTCACTTAA	AWYDFARAVHTNKHVNLLFT*	-1.434	0.001243	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25201	RHRSGWPCFGYNYFARHSMN			20	SLAY-screened peptide P3551			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCACCGGTCTGGCTGGCCGTGTTTTGGCTATAACTATTTCGCGCGCCATAGCATGAACTAA	RHRSGWPCFGYNYFARHSMN*	-1.433	0.0039	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25202	GFELSSPL			8	SLAY-screened peptide P3552			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTTCGAGCTTTCATCCCCACTCTGAGTGCCTTAGCGTCGAGAACAACGATCATTTCTGGTA	GFELSSPL*VP*RREQRSFLV	-1.433	0.002357	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25203	HEPAHGPTMYKFMMNTVARY			20	SLAY-screened peptide P3553			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGAGCCCGCCCATGGTCCGACCATGTACAAGTTCATGATGAACACCGTCGCCCGCTACTAA	HEPAHGPTMYKFMMNTVARY*	-1.433	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25204	ICCLRNSTKIRPKIIHHNQG			20	SLAY-screened peptide P3554			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTTGCTGCTTGCGGAACTCTACCAAGATCCGGCCCAAGATTATTCATCACAACCAGGGTTAA	ICCLRNSTKIRPKIIHHNQG*	-1.433	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25205	SHTPGNCWLISYHSMDPNTT			20	SLAY-screened peptide P3555			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCACACGCCTGGCAATTGCTGGCTGATTAGTTACCACTCGATGGATCCCAATACTACGTAA	SHTPGNCWLISYHSMDPNTT*	-1.433	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25206	GFHLRPAVGGGYNLTNTDST			20	SLAY-screened peptide P3556			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTTCCACCTGCGCCCGGCCGTCGGCGGTGGTTACAATCTTACTAACACCGATAGCACCTAA	GFHLRPAVGGGYNLTNTDST*	-1.432	0.009018	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25207	FTSPPCVPLYDPYAWQQISM			20	SLAY-screened peptide P3557			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCACCAGCCCTCCTTGCGTCCCTCTTTACGACCCTTACGCCTGGCAGCAGATCAGCATGTAA	FTSPPCVPLYDPYAWQQISM*	-1.432	0.031359	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25208	ADRVLPRYAHTSIAHPGRVG			20	SLAY-screened peptide P3558			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGATCGCGTTCTCCCTCGCTACGCGCATACGAGCATTGCTCATCCGGGCAGGGTTGGGTAA	ADRVLPRYAHTSIAHPGRVG*	-1.432	0.016039	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25209	NTCFTEPYHTDTKRNSHGEN			20	SLAY-screened peptide P3559			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATACCTGCTTCACTGAGCCTTACCACACTGATACTAAGAGGAACTCCCATGGCGAGAATTAA	NTCFTEPYHTDTKRNSHGEN*	-1.432	0.007081	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25210	RGSTRRPNARPLRPNNPAPT			20	SLAY-screened peptide P3560			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGGGCTCCACTCGGCGCCCTAACGCGCGTCCGCTGAGGCCTAATAACCCTGCTCCTACGTAA	RGSTRRPNARPLRPNNPAPT*	-1.431	0.000723	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25211	QPIKYDVSTVLLSYYDYNKA			20	SLAY-screened peptide P3561			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCTATCAAGTACGATGTTTCCACCGTGTTGCTTTCGTACTACGATTATAATAAGGCCTAA	QPIKYDVSTVLLSYYDYNKA*	-1.431	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25212	FSFYLDPPCYLPMNLYHHNY			20	SLAY-screened peptide P3562			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTCGTTTTACCTGGATCCTCCCTGTTACTTGCCGATGAACCTTTACCACCATAATTATTAA	FSFYLDPPCYLPMNLYHHNY*	-1.431	2.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25213	HQFSCQTGSIDRPRGR			16	SLAY-screened peptide P3563			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCAGTTCAGTTGCCAGACGGGTAGTATCGACCGCCCCAGGGGCCGTTAGGGCGTGCGCTAA	HQFSCQTGSIDRPRGR*GVR*	-1.431	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25214	LPSPHEKPVPASIQYTCTPL			20	SLAY-screened peptide P3564			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCCAGTCCGCACGAGAAGCCTGTTCCTGCGTCCATTCAGTATACTTGCACCCCCTTGTAA	LPSPHEKPVPASIQYTCTPL*	-1.431	0.009539	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25215	GMSLQCCPMIFAPSEYPYIY			20	SLAY-screened peptide P3565			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCATGTCTTTGCAGTGTTGCCCCATGATTTTTGCGCCTAGCGAGTATCCCTACATTTACTAA	GMSLQCCPMIFAPSEYPYIY*	-1.431	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25216	PGLSNNYAPNAIHTSPSKTI			20	SLAY-screened peptide P3566			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGGGTTGTCCAATAATTACGCTCCCAATGCCATTCATACGTCCCCGTCCAAGACTATTTAA	PGLSNNYAPNAIHTSPSKTI*	-1.431	0.018324	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25217	PKYFLTYPKPARHMFLRICS			20	SLAY-screened peptide P3567			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAAGTACTTTCTCACTTACCCCAAGCCCGCCCGTCACATGTTTCTGCGTATCTGCTCCTAA	PKYFLTYPKPARHMFLRICS*	-1.431	5.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25218	TSCSRACP			8	SLAY-screened peptide P3568			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCGTGCTCTCGCGCGTGTCCCTAGGCCAAGTCTATTGTCGCGACGTGTTGCTCCAAGTAA	TSCSRACP*AKSIVATCCSK*	-1.431	0.003145	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25219	YHGRLSPVSFNRNA			14	SLAY-screened peptide P3569			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCATGGGCGCCTGTCCCCTGTCTCTTTTAACCGTAACGCCTAGTCTTTCCCTTCCTCTTAA	YHGRLSPVSFNRNA*SFPSS*	-1.431	0.005804	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25220	NTPSYDSQDPTSSFCHQLRT			20	SLAY-screened peptide P3570			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATACCCCTAGCTACGATTCTCAGGATCCTACTTCTAGCTTCTGTCATCAGCTCCGTACTTAA	NTPSYDSQDPTSSFCHQLRT*	-1.43	0.011875	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25221	TTLDTDDNFQL			11	SLAY-screened peptide P3571			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTACCCTTGACACTGATGATAACTTCCAGCTTTAGTCTTCTATCAACAGGACGAATCTGTAA	TTLDTDDNFQL*SSINRTNL*	-1.43	0.047051	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25222	FNDHYCP			7	SLAY-screened peptide P3572			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAATGACCATTATTGCCCATGATCATCACTCTCGCCAGTACGGGGTCTAACTGAGTAAGTC	FNDHYCP*SSLSPVRGLTE*V	-1.43	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25223	GTHTPFLHPIFDPCGDLSPH			20	SLAY-screened peptide P3573			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTACCCATACCCCCTTCCTGCATCCGATTTTCGACCCTTGCGGTGACCTCAGCCCTCATTAA	GTHTPFLHPIFDPCGDLSPH*	-1.43	0.018603	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25224	SPAFVCHALSRSDFRLP			17	SLAY-screened peptide P3574			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCCGGCTTTCGTGTGCCATGCCCTTTCCCGGAGTGACTTTCGTTTGCCTTAGGCCCGGTAA	SPAFVCHALSRSDFRLP*AR*	-1.43	0.000399	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25225	PTTMACSWI			9	SLAY-screened peptide P3575			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACGACTATGGCCTGCTCGTGGATCTAGCTTCCCTCTTCCCAGTATATCAGTGTTAGTTAA	PTTMACSWI*LPSSQYISVS*	-1.43	7.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25226	FRGTQIMW			8	SLAY-screened peptide P3576			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCGGGGCACCCAGATCATGTGGTAGCAGTGGCTCATGATCAAGATGACGTGCTTTGATTAA	FRGTQIMW*QWLMIKMTCFD*	-1.43	8.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25227	HQVIVHASLSLCLNTVMLVC			20	SLAY-screened peptide P3577			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCAGGTTATTGTGCACGCGAGTCTGAGCCTTTGTCTCAATACCGTGATGCTTGTGTGCTAA	HQVIVHASLSLCLNTVMLVC*	-1.429	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25228	QCSNTFLPYRIADRWGDNSY			20	SLAY-screened peptide P3578			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTGCAGTAACACCTTCTTGCCTTACAGGATCGCTGATAGGTGGGGCGACAATTCTTATTAA	QCSNTFLPYRIADRWGDNSY*	-1.429	0.003856	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25229	RQPMWRSLQVYPAYAPFVTS			20	SLAY-screened peptide P3579			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCAGCCTATGTGGCGCTCCCTCCAGGTGTACCCCGCTTACGCCCCCTTCGTCACCTCCTAA	RQPMWRSLQVYPAYAPFVTS*	-1.429	0.006476	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25230	NYHQYPSFTTSSTKHVNSAM			20	SLAY-screened peptide P3580			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTACCATCAGTATCCGTCGTTTACTACGAGTTCTACGAAGCACGTCAATTCCGCGATGTAA	NYHQYPSFTTSSTKHVNSAM*	-1.429	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25231	PSRFCVTYRPKHVLSTTMVY			20	SLAY-screened peptide P3581			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCCCGGTTCTGTGTCACTTATAGGCCCAAGCACGTCTTGTCCACTACCATGGTCTACTAA	PSRFCVTYRPKHVLSTTMVY*	-1.428	0.001048	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25232	HIPNIYNNPISRWVGLVTIVN			21	SLAY-screened peptide P3582			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACATTCCTAATATTTATAATAACCCTATCTCTCGTTGGGTCGGTCTGGTTACAATCGTTAAC	HIPNIYNNPISRWVGLVTIVN	-1.428	0.036164	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25233	PSVLLFQHSLDQ			12	SLAY-screened peptide P3583			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTCGGTATTACTTTTTCAGCACTCGTTAGATCAGTAGTTTTGAGTGTCCTTAGAATGGCTA	PSVLLFQHSLDQ*F*VSLEWL	-1.428	0.016075	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25234	SNQTFINLHPGNGYFSLSNR			20	SLAY-screened peptide P3584			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGAATCAGACGTTCATCAACTTGCACCCCGGCAACGGTTACTTCTCGCTTAGTAACCGCTAA	SNQTFINLHPGNGYFSLSNR*	-1.428	0.021537	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25235	LTRTSVRTISTFRRRTN			17	SLAY-screened peptide P3585			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACGCGTACCAGTGTTCGGACCATCAGTACTTTCCGCCGTAGGACGAACTAGAGGTATTAA	LTRTSVRTISTFRRRTN*RY*	-1.428	0.024916	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25236	TSSQHTPPIPEVIPAGYHNY			20	SLAY-screened peptide P3586			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCGTCCCAGCACACGCCCCCTATCCCTGAGGTGATCCCTGCGGGGTACCATAATTATTAA	TSSQHTPPIPEVIPAGYHNY*	-1.428	0.006173	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25237	PAFMSTPNLQLRFRHTLS			18	SLAY-screened peptide P3587			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGCGTTCATGTCCACTCCGAACCTTCAGCTCAGGTTTCGGCATACTTTGTCTTAGATGTAA	PAFMSTPNLQLRFRHTLS*M*	-1.427	0.000278	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25238	LLQLIDLTFMLTVRNARSKN			20	SLAY-screened peptide P3588			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCTTCAGCTGATCGATCTGACTTTTATGTTGACGGTGCGTAATGCGCGCAGCAAGAACTAA	LLQLIDLTFMLTVRNARSKN*	-1.427	0.000511	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25239	LHPTHNLNSEPRYIPLYITT			20	SLAY-screened peptide P3589			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCACCCTACCCATAACTTGAATAGCGAGCCGCGCTACATTCCTCTGTACATTACGACTTAA	LHPTHNLNSEPRYIPLYITT*	-1.427	0.011791	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25240	LLTHTFHNGYYDETSDVAYG			20	SLAY-screened peptide P3590			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCTGACTCATACGTTCCATAACGGTTACTACGACGAGACGAGCGACGTCGCTTACGGCTAA	LLTHTFHNGYYDETSDVAYG*	-1.427	0.000186	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25241	SSYRPRPITPPTGAWIAQCH			20	SLAY-screened peptide P3591			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCAGTTACCGGCCTCGGCCCATTACTCCGCCCACTGGTGCTTGGATCGCCCAGTGTCACTAA	SSYRPRPITPPTGAWIAQCH*	-1.427	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25242	SIYYQTHICV			10	SLAY-screened peptide P3592			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGATCTATTATCAGACTCATATTTGCGTGTAGTCCCACTCTTACTGCTATGGTATTTCCTAA	SIYYQTHICV*SHSYCYGIS*	-1.427	0.00338	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25243	LLAADRSMEISVSAPDMNLT			20	SLAY-screened peptide P3593			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTGGCTGCCGACCGTTCCATGGAGATCTCTGTCTCCGCGCCCGATATGAACCTTACGTAA	LLAADRSMEISVSAPDMNLT*	-1.427	0.000721	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25244	AAAYFNEYRRIPRYGSDACF			20	SLAY-screened peptide P3594			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCGGCGTACTTTAACGAGTACCGCAGGATCCCGAGGTACGGCTCTGACGCGTGCTTTTAA	AAAYFNEYRRIPRYGSDACF*	-1.427	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25245	SGTRCCACHCNPALPRMQYI			20	SLAY-screened peptide P3595			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGGTACCCGTTGTTGCGCTTGTCATTGCAACCCCGCGTTGCCCAGGATGCAGTATATCTAA	SGTRCCACHCNPALPRMQYI*	-1.426	0.003184	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25246	VNCASSIFSITFRLMIRITLN			21	SLAY-screened peptide P3596			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTAACTGCGCAAGCTCTATTTTCTCAATCACATTTCGTTTAATGATCCGAATTACACTTAAC	VNCASSIFSITFRLMIRITLN	-1.426	0.00027	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25247	PRLHTSHICTILHHAPWSCH			20	SLAY-screened peptide P3597			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGGCTTCACACTAGCCACATTTGCACGATCCTGCACCATGCTCCTTGGTCGTGCCACTAA	PRLHTSHICTILHHAPWSCH*	-1.426	0.000267	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25248	RPLCVPWSSCFPIYSPYCMF			20	SLAY-screened peptide P3598			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCGCTCTGCGTCCCCTGGAGTTCTTGTTTCCCCATTTATTCTCCTTACTGTATGTTCTAA	RPLCVPWSSCFPIYSPYCMF*	-1.426	0.000568	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25249	LASAVDPYKCYSTLGNNHSR			20	SLAY-screened peptide P3599			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGGCTTCGGCTGTCGACCCCTACAAGTGTTATTCGACCTTGGGGAATAATCACAGTAGGTAA	LASAVDPYKCYSTLGNNHSR*	-1.426	0.005426	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25250	SPETLPPSSSFRDSCRASCS			20	SLAY-screened peptide P3600			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCCCGAGACTCTGCCGCCTTCTTCTAGTTTTCGGGACTCGTGCCGGGCTAGTTGCTCGTAA	SPETLPPSSSFRDSCRASCS*	-1.426	0.001565	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25251	ITNSLYPATYTIATPFPVRV			20	SLAY-screened peptide P3601			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCACCAACTCGTTGTACCCTGCTACCTATACTATTGCCACCCCGTTTCCCGTCCGGGTGTAA	ITNSLYPATYTIATPFPVRV*	-1.426	0.00909	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25252	AWGQPLYHARTSYDCWAHSA			20	SLAY-screened peptide P3602			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTGGGGCCAGCCGCTTTACCACGCTCGTACCAGCTACGATTGCTGGGCGCATTCTGCGTAA	AWGQPLYHARTSYDCWAHSA*	-1.425	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25253	RGHPTATDSVGNKISIIVALN			21	SLAY-screened peptide P3603			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGGTCATCCTACTGCGACCGATAGCGTGGGCAACAAGATATCCATTATAGTCGCACTTAAC	RGHPTATDSVGNKISIIVALN	-1.425	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25254	RPSSPSVDAAWTRCPTYRSF			20	SLAY-screened peptide P3604			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCCAGTTCTCCGAGTGTGGATGCGGCTTGGACCCGTTGTCCCACGTATCGGTCTTTTTAA	RPSSPSVDAAWTRCPTYRSF*	-1.425	0.00087	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25255	QILYCNKTHDHDQCLT			16	SLAY-screened peptide P3605			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGATCTTGTACTGTAACAAGACGCACGACCATGATCAGTGCCTCACCTAGGATGAGCAGTAA	QILYCNKTHDHDQCLT*DEQ*	-1.425	3.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25256	HSS			3	SLAY-screened peptide P3606			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTCCTCCTAGCTGAATGGCTCCGAGTCTAGTCTCCTGTTCTTTAGTCACGAGACCCTTTAA	HSS*LNGSESSLLFFSHETL*	-1.425	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25257	RAICYDDLTAPVLLVYYPCM			20	SLAY-screened peptide P3607			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCCATTTGCTATGATGACCTTACGGCCCCTGTTCTGTTGGTCTACTACCCTTGTATGTAA	RAICYDDLTAPVLLVYYPCM*	-1.425	0.000212	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25258	LLFCTNGSLQPSSGAPIFTC			20	SLAY-screened peptide P3608			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTGTTTTGTACGAATGGGTCCCTGCAGCCCTCTTCCGGTGCCCCTATCTTCACTTGTTAA	LLFCTNGSLQPSSGAPIFTC*	-1.425	2.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25259	KSFMAVPLSRRATSHTW			17	SLAY-screened peptide P3609			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGTCCTTCATGGCCGTCCCCCTCTCTAGGCGCGCTACTTCTCACACTTGGTAGGTTTGTTAA	KSFMAVPLSRRATSHTW*VC*	-1.424	0.000204	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25260	RHHHTSAHDISDGRRSHGID			20	SLAY-screened peptide P3610			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCACCACCACACGTCTGCTCACGACATTAGCGATGGTCGCCGTTCTCATGGTATTGACTAA	RHHHTSAHDISDGRRSHGID*	-1.424	0.000189	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25261	IFNICVSPPLYGWINHQIIT			20	SLAY-screened peptide P3611			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTTTTAATATTTGCGTCTCTCCTCCCCTGTACGGGTGGATCAATCACCAGATTATCACCTAA	IFNICVSPPLYGWINHQIIT*	-1.424	0.017581	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25262	PRSAPTLWGPNDGHCRPAQS			20	SLAY-screened peptide P3612			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGCTCGGCCCCTACTCTCTGGGGGCCCAACGATGGGCATTGCCGGCCGGCGCAGTCCTAA	PRSAPTLWGPNDGHCRPAQS*	-1.424	0.003167	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25263	CSVPPTYDTYIYSIRSLSFL			20	SLAY-screened peptide P3613			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTCTGTGCCGCCGACCTACGACACTTACATCTATTCCATCCGTTCTCTTTCTTTTCTCTAA	CSVPPTYDTYIYSIRSLSFL*	-1.423	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25264	LGLLSNSNYLELLYCFMGRN			20	SLAY-screened peptide P3614			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGGGTTGCTTTCTAATTCGAATTACCTTGAGTTGTTGTATTGCTTTATGGGGCGTAACTAA	LGLLSNSNYLELLYCFMGRN*	-1.423	0.025719	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25265	RCPYPFRLTCPCHYIVNKTD			20	SLAY-screened peptide P3615			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGTCCTTATCCCTTCCGGCTGACTTGCCCCTGCCACTATATTGTCAATAAGACTGATTAA	RCPYPFRLTCPCHYIVNKTD*	-1.423	0.000641	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25266	SSARDSSVNNV			11	SLAY-screened peptide P3616			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTCCGCGAGGGACTCGAGCGTGAATAACGTCTAGCTGACTATTCTGTAGCGGATGAGTTAA	SSARDSSVNNV*LTIL*RMS*	-1.423	0.001833	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25267	LHYESMCPATSNP			13	SLAY-screened peptide P3617			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCACTACGAGTCTATGTGCCCTGCTACTTCTAACCCTTAGCCTTCCAACCATACCACGTAA	LHYESMCPATSNP*PSNHTT*	-1.423	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25268	AAGTSGFYYFCCAFQFKDRS			20	SLAY-screened peptide P3618			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCGGGCACTTCTGGTTTCTACTATTTTTGTTGTGCTTTTCAGTTCAAGGATCGCTCTTAA	AAGTSGFYYFCCAFQFKDRS*	-1.423	0.003047	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25269	CHIGEVADGSLFQNNNRNIH			20	SLAY-screened peptide P3619			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCACATCGGTGAGGTCGCGGATGGTTCTTTGTTTCAGAATAATAATCGGAATATTCACTAA	CHIGEVADGSLFQNNNRNIH*	-1.423	0.000228	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25270	PSGCTSNFNWTLIAAPLFNH			20	SLAY-screened peptide P3620			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGCGGGTGTACTTCCAATTTCAATTGGACGCTGATTGCCGCGCCCTTGTTTAATCACTAA	PSGCTSNFNWTLIAAPLFNH*	-1.422	0.001212	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25271	PNSNSLTRYSTTTTHLFCFR			20	SLAY-screened peptide P3621			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAACAGTAACTCTCTTACTCGTTATAGCACGACCACTACCCATTTGTTCTGCTTCAGGTAA	PNSNSLTRYSTTTTHLFCFR*	-1.422	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25272	SFIVLPNFCYDLASAWPMLG			20	SLAY-screened peptide P3622			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTTCATTGTCCTCCCCAATTTTTGCTATGACTTGGCGTCGGCTTGGCCCATGCTGGGGTAA	SFIVLPNFCYDLASAWPMLG*	-1.422	0.006832	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25273	RLRASIANFTTKLCSSSNSL			20	SLAY-screened peptide P3623			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTTGCGGGCCTCCATTGCCAATTTCACTACTAAGCTGTGTTCTTCTAGTAACTCTCTCTAA	RLRASIANFTTKLCSSSNSL*	-1.422	0.024365	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25274	RYRVSTTVSFFD			12	SLAY-screened peptide P3624			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTACCGCGTTTCGACTACCGTTAGCTTCTTCGACTAGTCCTTCATGACCGGTTGGCCCTAA	RYRVSTTVSFFD*SFMTGWP*	-1.422	0.004321	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25275	STPITYHCRIRIQFDMRLRIY			21	SLAY-screened peptide P3625			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCACTCCGATCACTTATCATTGTCGCATTCGCATCCAGTTCGACATGCGCTTGAGGATCTAC	STPITYHCRIRIQFDMRLRIY	-1.421	0.002071	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25276	PFPCFNIFPRSYGCCDSIYR			20	SLAY-screened peptide P3626			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTTCCTTGCTTCAACATTTTCCCCCGCAGTTATGGCTGCTGTGACTCCATTTACCGCTAA	PFPCFNIFPRSYGCCDSIYR*	-1.421	0.029554	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25277	SAPGAGGRVTALF			13	SLAY-screened peptide P3627			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCTCCTGGCGCCGGGGGGCGCGTCACTGCGCTTTTTTAGTATACCGACCACGTGCCCTAA	SAPGAGGRVTALF*YTDHVP*	-1.421	0.006245	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25278	PSGAALWGVQSSATSPPILC			20	SLAY-screened peptide P3628			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTCGGGCGCGGCGTTGTGGGGTGTGCAGTCGAGTGCTACCAGCCCGCCTATCCTTTGCTAA	PSGAALWGVQSSATSPPILC*	-1.421	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25279	NPNSLYSAHILTNSRPSEIT			20	SLAY-screened peptide P3629			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCCTAATTCCCTTTATTCTGCTCACATTCTTACCAATAGCCGGCCCAGTGAGATCACGTAA	NPNSLYSAHILTNSRPSEIT*	-1.421	0.004391	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25280	RRTCVPVYHVS			11	SLAY-screened peptide P3630			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGTACTTGCGTCCCCGTGTATCACGTGAGCTAGCCCTCTATGTGTCCCGTCCGCCGCTAA	RRTCVPVYHVS*PSMCPVRR*	-1.421	0.000437	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25281	PGLLANGPFSYGVLALMPFFY			21	SLAY-screened peptide P3631			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGCCTTCTCGCCAATGGTCCCTTTAGTTACGGCGTCCTCGCCCTTATGCCCTTTTTCTAC	PGLLANGPFSYGVLALMPFFY	-1.421	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25282	ALAFSHRHTTPGSVDAVPAS			20	SLAY-screened peptide P3632			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCTGGCTTTTTCCCACCGTCATACCACGCCCGGCTCTGTCGACGCCGTCCCCGCTAGTTAA	ALAFSHRHTTPGSVDAVPAS*	-1.42	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25283	NPVCSPVTVVATWSPSLRTPN			21	SLAY-screened peptide P3633			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCCGGTGTGTAGCCCGGTAACTGTTGTTGCTACTTGGAGCCCGTCTTTACGTACGCCTAAC	NPVCSPVTVVATWSPSLRTPN	-1.42	0.003657	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25284	GVGTTTHVYLSEYSATALAD			20	SLAY-screened peptide P3634			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGTCGGGACGACGACCCATGTCTACCTCTCCGAGTATTCGGCGACTGCCCTGGCTGATTAA	GVGTTTHVYLSEYSATALAD*	-1.42	0.005051	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25285	LFAVDLNGPNPCSYSSHPMV			20	SLAY-screened peptide P3635			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTTTGCGGTCGACCTTAATGGGCCCAACCCCTGCAGCTATTCTTCTCATCCTATGGTGTAA	LFAVDLNGPNPCSYSSHPMV*	-1.42	0.000115	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25286	MVLMNPIVEICPSVAMSQLE			20	SLAY-screened peptide P3636			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGTTCTGATGAACCCCATCGTCGAGATCTGCCCTTCCGTCGCGATGAGTCAGCTTGAGTAA	MVLMNPIVEICPSVAMSQLE*	-1.42	0.000547	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25287	RVARILHGLNIRFMRKSKPD			20	SLAY-screened peptide P3637			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGTGGCTCGCATCCTGCATGGTCTCAATATTCGCTTTATGCGTAAGAGTAAGCCTGATTAA	RVARILHGLNIRFMRKSKPD*	-1.42	7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25288	TGIKFTDHFGSSGQNPFACV			20	SLAY-screened peptide P3638			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGGTATTAAGTTTACTGATCATTTTGGCAGTAGTGGGCAGAACCCGTTTGCCTGTGTTTAA	TGIKFTDHFGSSGQNPFACV*	-1.42	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25289	MDPLWADSMLL			11	SLAY-screened peptide P3639			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGATCCTCTTTGGGCGGACTCTATGCTTTTGTAGGAGATTCTGCCGACTCCCCAGCATTAA	MDPLWADSMLL*EILPTPQH*	-1.419	0.000365	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25290	PSL			3	SLAY-screened peptide P3640			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTCCCTTTAGGTTTGCCCGACCATGAACGCCTCTGCCCATCCCCAGTAGGAGAATGGGTAA	PSL*VCPTMNASAHPQ*ENG*	-1.419	0.002457	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25291	PDVAASAHTIPFPCGTIELL			20	SLAY-screened peptide P3641			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGATGTCGCCGCCTCGGCTCATACTATTCCTTTCCCGTGTGGTACCATTGAGCTGCTTTAA	PDVAASAHTIPFPCGTIELL*	-1.419	0.027227	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25292	CMRYLLVDRTDASPSHNFNY			20	SLAY-screened peptide P3642			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTATGCGCTATCTTCTCGTGGATCGCACGGATGCGTCTCCTAGCCATAACTTTAATTACTAA	CMRYLLVDRTDASPSHNFNY*	-1.419	6.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25293	PAGVHYHCMTAFGDI			15	SLAY-screened peptide P3643			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGCGGGGGTGCACTACCATTGCATGACCGCTTTCGGGGACATCTAGCCGCCCCATTACTAA	PAGVHYHCMTAFGDI*PPHY*	-1.419	0.028323	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25294	TFETCPQEHSYISSSAPDTS			20	SLAY-screened peptide P3644			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTTTGAGACCTGCCCCCAGGAGCATTCTTACATCAGTAGCTCTGCCCCCGATACTTCCTAA	TFETCPQEHSYISSSAPDTS*	-1.419	0.001507	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25295	DWLRCGHNCNACPFQLWERS			20	SLAY-screened peptide P3645			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTGGCTCCGCTGCGGTCATAATTGCAATGCTTGCCCTTTCCAGCTTTGGGAGCGCTCGTAA	DWLRCGHNCNACPFQLWERS*	-1.419	0.009628	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25296	ETFIPSSTALPDHASYHLHL			20	SLAY-screened peptide P3646			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGACCTTTATCCCCTCCTCGACTGCTCTGCCGGATCACGCGTCCTACCATTTGCATTTGTAA	ETFIPSSTALPDHASYHLHL*	-1.419	0.043905	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25297	LQDFTFSTPLSSQLLSYDGR			20	SLAY-screened peptide P3647			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCAGGATTTTACCTTCAGTACGCCTCTTTCGTCCCAGCTTCTGTCTTACGATGGTCGTTAA	LQDFTFSTPLSSQLLSYDGR*	-1.419	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25298	HAVSNAGPRIPGYLVPIVCD			20	SLAY-screened peptide P3648			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGCGGTCAGTAACGCGGGGCCGCGGATCCCTGGTTATCTTGTGCCCATCGTCTGTGACTAA	HAVSNAGPRIPGYLVPIVCD*	-1.419	0.000912	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25299	EEATSVSLLLPYRNTYPDAC			20	SLAY-screened peptide P3649			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGAGGCGACGTCCGTGTCTCTTTTGCTTCCGTACCGTAACACTTACCCTGACGCGTGTTAA	EEATSVSLLLPYRNTYPDAC*	-1.419	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25300	PNNHWPTLFPASRFGPCYAF			20	SLAY-screened peptide P3650			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAACAATCATTGGCCTACTCTCTTCCCCGCGTCCAGGTTTGGCCCCTGCTACGCCTTCTAA	PNNHWPTLFPASRFGPCYAF*	-1.419	0.008337	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25301	MSPRPWFPLTVLYLTYFEDT			20	SLAY-screened peptide P3651			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTCTCCCCGTCCTTGGTTCCCTCTGACCGTTCTTTACCTTACCTACTTCGAGGATACCTAA	MSPRPWFPLTVLYLTYFEDT*	-1.418	0.007213	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25302	AMRDVGGLFSRILRSILRLIN			21	SLAY-screened peptide P3652			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCATGCGTGATGTGGGCGGCTTGTTCAGCAGAATTTTAAGATCAATACTTAGATTGATTAAC	AMRDVGGLFSRILRSILRLIN	-1.418	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25303	DATRNPRLSIQTNA			14	SLAY-screened peptide P3653			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATGCTACCCGGAATCCGAGGCTCTCCATTCAGACGAACGCTTAGTTTAATACTGTTGGTTAA	DATRNPRLSIQTNA*FNTVG*	-1.418	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25304	GVYKVAE			7	SLAY-screened peptide P3654			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGGTGTATAAGGTGGCGGAGTAGCGGCGCAACACGGACGTGGACACTTTTAACGGTAGTTAA	GVYKVAE*RRNTDVDTFNGS*	-1.417	0.034218	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25305	LVGANFSETCPLND			14	SLAY-screened peptide P3655			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGGTTGGTGCTAACTTTAGTGAGACTTGCCCTCTGAATGATTAGACTCCTCATAACGGGTAA	LVGANFSETCPLND*TPHNG*	-1.417	0.028268	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25306	GSYYHDMSFRHPNPPTG			17	SLAY-screened peptide P3656			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGTCTTACTACCATGACATGAGCTTTCGGCATCCGAACCCCCCCACCGGTTAGAATACTTAA	GSYYHDMSFRHPNPPTG*NT*	-1.416	7.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25307	HRSHNSTVYSYVRNRYIPTG			20	SLAY-screened peptide P3657			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCGTTCGCATAACTCCACTGTTTACTCGTACGTTAGGAACCGTTATATTCCCACCGGTTAA	HRSHNSTVYSYVRNRYIPTG*	-1.416	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25308	VEIPASRLLPHRPGHHHSIY			20	SLAY-screened peptide P3658			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGAGATTCCCGCTAGCCGCCTTCTTCCGCATAGGCCTGGCCATCATCATAGTATTTATTAA	VEIPASRLLPHRPGHHHSIY*	-1.416	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25309	TDNTSHNAGSRKNIQFHGESL			21	SLAY-screened peptide P3659			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGACAACACTAGCCATAATGCTGGTAGTCGTAAGAATATTCAATTTCACGGTGAATCCCTA	TDNTSHNAGSRKNIQFHGESL	-1.416	0.000895	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25310	LLNQFSTDAHRRLHWLSYSK			20	SLAY-screened peptide P3660			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTTAACCAGTTCTCGACCGACGCGCACCGGCGCCTTCACTGGCTTTCTTACAGCAAGTAA	LLNQFSTDAHRRLHWLSYSK*	-1.415	0.004536	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25311	CRMTITSGASCP			12	SLAY-screened peptide P3661			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCGTATGACCATTACTAGTGGGGCGAGCTGTCCTTAGAGCGACTGCTAGAATATGAGCTAA	CRMTITSGASCP*SDC*NMS*	-1.415	0.002002	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25312	LTRAPHAASHCFNTLNSLDA			20	SLAY-screened peptide P3662			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACTAGGGCCCCCCACGCGGCGTCCCACTGCTTCAACACCCTGAACTCTCTGGATGCGTAA	LTRAPHAASHCFNTLNSLDA*	-1.415	0.000139	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25313	KNLNTVQPSPAHIPIPRRTAN			21	SLAY-screened peptide P3663			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGAACCTTAACACCGTGCAGCCGTCTCCTGCGCACATTCCTATTCCCAGACGGACCGCTAAC	KNLNTVQPSPAHIPIPRRTAN	-1.415	0.04411	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25314	RIHTRDQPRQPVFRCDLNAD			20	SLAY-screened peptide P3664			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTATCCACACCAGGGACCAGCCCCGTCAGCCCGTCTTCAGGTGCGATCTGAATGCTGACTAA	RIHTRDQPRQPVFRCDLNAD*	-1.415	2.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25315	LIWSDLQQASYRHNSGFFVP			20	SLAY-screened peptide P3665			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATCTGGTCCGACTTGCAGCAGGCCAGCTATCGCCACAATAGTGGTTTCTTCGTTCCCTAA	LIWSDLQQASYRHNSGFFVP*	-1.415	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25316	WIRRWVYSSFQNRTPTRKGS			20	SLAY-screened peptide P3666			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGATCCGCCGGTGGGTGTATTCGAGCTTTCAGAATCGCACCCCCACTCGGAAGGGGTCCTAA	WIRRWVYSSFQNRTPTRKGS*	-1.414	7.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25317	VPDAVPLNATWSQTQSYKRV			20	SLAY-screened peptide P3667			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCCTGACGCCGTGCCGCTCAACGCTACTTGGAGTCAGACCCAGTCCTATAAGAGGGTGTAA	VPDAVPLNATWSQTQSYKRV*	-1.414	0.002377	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25318	RWLLPTAVNNCISTPVFWRGN			21	SLAY-screened peptide P3668			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTGGCTGTTGCCCACCGCGGTCAACAATTGTATCTCGACACCTGTTTTCTGGAGGGGTAAC	RWLLPTAVNNCISTPVFWRGN	-1.414	0.005225	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25319	PCFYVVSIIPWLYDYSMLSC			20	SLAY-screened peptide P3669			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGTTTCTATGTCGTCTCTATTATTCCTTGGCTCTATGACTACTCCATGCTGTCCTGCTAA	PCFYVVSIIPWLYDYSMLSC*	-1.414	0.000402	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25320	IGMANVKCTHTHRWTFAMST			20	SLAY-screened peptide P3670			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGGTATGGCCAACGTGAAGTGCACGCACACCCACCGCTGGACTTTCGCCATGTCTACGTAA	IGMANVKCTHTHRWTFAMST*	-1.414	0.019065	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25321	VCPDAYLTASLCDRYNFAA			19	SLAY-screened peptide P3671			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTGCCCTGACGCGTACCTTACCGCGAGCCTTTGTGATAGGTACAATTTTGCTGCTTAGTAA	VCPDAYLTASLCDRYNFAA**	-1.413	0.00172	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25322	HPNLTNICFQHEILDYDLLM			20	SLAY-screened peptide P3672			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCTAATCTCACCAACATCTGTTTCCAGCACGAGATTTTGGACTACGACCTTCTGATGTAA	HPNLTNICFQHEILDYDLLM*	-1.413	0.007148	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25323	PWLYLTDLAHHRTPSLNTAD			20	SLAY-screened peptide P3673			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGGTTGTACTTGACTGACCTGGCTCACCACCGTACTCCGTCTCTTAACACCGCCGATTAA	PWLYLTDLAHHRTPSLNTAD*	-1.413	0.000126	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25324	AYTDFVLRLISPSCTASMYL			20	SLAY-screened peptide P3674			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTATACCGATTTCGTTCTGAGGTTGATCAGCCCCAGCTGTACGGCCAGTATGTATCTCTAA	AYTDFVLRLISPSCTASMYL*	-1.413	0.000676	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25325	IDGTQMDACL			10	SLAY-screened peptide P3675			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGACGGCACTCAGATGGATGCCTGTTTGTAGAAGATTGGGTGTCTCCACCGGAATTTCTAA	IDGTQMDACL*KIGCLHRNF*	-1.413	7.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25326	PMNIIRCYPINWTT			14	SLAY-screened peptide P3676			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATGAACATTATCCGTTGCTACCCGATTAATTGGACTACGTAGGAGCTTTAGTTTCCCTAA	PMNIIRCYPINWTT*EL*FP*	-1.413	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25327	HWTPQKCYDIFPDHSVIHFV			20	SLAY-screened peptide P3677			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGGACTCCGCAGAAGTGCTACGACATTTTCCCCGACCATTCTGTCATTCACTTTGTTTAA	HWTPQKCYDIFPDHSVIHFV*	-1.412	0.026328	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25328	SPHWAHAEQDVP			12	SLAY-screened peptide P3678			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCCTCATTGGGCTCACGCTGAGCAGGACGTTCCCTAATAATCTTACCTTGTAACTGAGTAA	SPHWAHAEQDVP**SYLVTE*	-1.412	0.027354	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25329	WPILCPNRYIIISYANAGLTY			21	SLAY-screened peptide P3679			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCCCATCCTGTGCCCCAACAGGTACATTATTATCAGTTACGCCAACGCCGGGCTTACGTAC	WPILCPNRYIIISYANAGLTY	-1.412	0.009928	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25330	NYSEGVEKPVFECIALHNFR			20	SLAY-screened peptide P3680			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTACAGCGAGGGCGTGGAGAAGCCCGTGTTTGAGTGTATCGCTCTTCATAATTTCCGTTAA	NYSEGVEKPVFECIALHNFR*	-1.412	0.023023	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25331	LRATPAERLSHVNSITNVMV			20	SLAY-screened peptide P3681			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCGCGCCACGCCCGCTGAGCGTCTCTCTCACGTTAACTCGATCACTAACGTCATGGTCTAA	LRATPAERLSHVNSITNVMV*	-1.412	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25332	PPWLHSHAHYSTCVYPTGTT			20	SLAY-screened peptide P3682			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCCTGGCTTCACTCCCATGCCCACTATTCTACCTGTGTTTATCCTACGGGTACGACGTAA	PPWLHSHAHYSTCVYPTGTT*	-1.411	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25333	PIYPYDKYRYSNCPFYYVDI			20	SLAY-screened peptide P3683			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATTTATCCGTACGACAAGTATCGGTATAGCAATTGTCCGTTTTATTATGTTGATATCTAA	PIYPYDKYRYSNCPFYYVDI*	-1.411	0.002264	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25334	DCDPYLPSTPYPLTLHHWII			20	SLAY-screened peptide P3684			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTGTGATCCTTATCTTCCGTCGACGCCCTATCCTCTGACCCTCCACCACTGGATCATCTAA	DCDPYLPSTPYPLTLHHWII*	-1.411	0.003721	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25335	LSQAPSRLCYCSYTYNTEMQ			20	SLAY-screened peptide P3685			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGAGTCAGGCCCCTAGCCGTTTGTGCTACTGCTCTTATACCTACAACACTGAGATGCAGTAA	LSQAPSRLCYCSYTYNTEMQ*	-1.411	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25336	DPGHKTMLDFLACPLLTRLL			20	SLAY-screened peptide P3686			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCCCGGCCACAAGACGATGCTCGACTTCCTGGCCTGTCCTCTGCTCACTAGGCTTCTCTAA	DPGHKTMLDFLACPLLTRLL*	-1.411	0.000161	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25337	NLVT			4	SLAY-screened peptide P3687			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTTGGTCACTTAGAGCACCATGAGCCTGGGCATTACATCCATGGGTATCCGTCTGCCTAAC	NLVT*STMSLGITSMGIRLPN	-1.41	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25338	YSPSHAWSISDKTTSKQIND			20	SLAY-screened peptide P3688			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTCGCCGAGTCATGCCTGGAGTATCAGCGATAAGACGACGAGCAAGCAGATTAATGATTAA	YSPSHAWSISDKTTSKQIND*	-1.41	0.00465	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25339	PGFSYYIILCYDLFTSSRTS			20	SLAY-screened peptide P3689			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGCTTCAGCTATTATATTATCCTTTGTTATGACCTTTTCACTAGTTCCCGCACCTCTTAA	PGFSYYIILCYDLFTSSRTS*	-1.41	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25340	PGPTSHKLRKRQSWHFDGSP			20	SLAY-screened peptide P3690			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGGGCCCACTTCTCATAAGTTGCGTAAGCGCCAGAGTTGGCATTTTGACGGGAGTCCCTAA	PGPTSHKLRKRQSWHFDGSP*	-1.41	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25341	ACVPST			6	SLAY-screened peptide P3691			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTGCGTTCCCTCCACCTAGTGTCCGTTTGTGAGCGGTTTTACTCAGTTCTTGTGTAGGTAA	ACVPST*CPFVSGFTQFLCR*	-1.41	0.003652	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25342	RCSPLIYLSHNHAYHTPPAL			20	SLAY-screened peptide P3692			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTGCAGTCCTCTTATCTACCTCAGCCATAACCATGCTTATCACACCCCCCCGGCCTTGTAA	RCSPLIYLSHNHAYHTPPAL*	-1.41	0.023556	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25343	SPVLTATAWVNRITPYTPGV			20	SLAY-screened peptide P3693			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCCCGTTTTGACCGCGACCGCGTGGGTCAACCGCATTACTCCCTATACCCCTGGTGTCTAA	SPVLTATAWVNRITPYTPGV*	-1.41	0.006406	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25344	MHLNCNDFTPVHGSY			15	SLAY-screened peptide P3694			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCACCTGAATTGTAACGATTTTACTCCGGTTCATGGTTCCTACTAGATGCGCGCTTGGTAA	MHLNCNDFTPVHGSY*MRAW*	-1.409	0.014383	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25345	CRSRTSTAEQNTSVHRGTII			20	SLAY-screened peptide P3695			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAGGAGTCGGACTTCGACCGCCGAGCAGAATACTTCCGTTCACCGCGGTACTATTATTTAA	CRSRTSTAEQNTSVHRGTII*	-1.409	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25346	RPSAPSQDVQTGSAITLIFH			20	SLAY-screened peptide P3696			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCGTCCGCGCCGTCCCAGGACGTTCAGACGGGCAGCGCCATCACGCTCATTTTTCACTAA	RPSAPSQDVQTGSAITLIFH*	-1.409	0.000973	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25347	LSTANYPHLP			10	SLAY-screened peptide P3697			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCCACGGCGAACTATCCCCACCTCCCCTAGCCCCTGAACTCTAAGAGCCCGCGCCTTTAA	LSTANYPHLP*PLNSKSPRL*	-1.409	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25348	LLGAVMALLTHVQNLKRINR			20	SLAY-screened peptide P3698			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTTGGGCGCTGTGATGGCTCTCCTCACCCACGTTCAGAACCTGAAGCGGATTAATCGGTAA	LLGAVMALLTHVQNLKRINR*	-1.409	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25349	ASRARFNTLAIIPPRCNITT			20	SLAY-screened peptide P3699			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAGCCGCGCCCGCTTCAACACTCTCGCCATTATCCCCCCTCGCTGTAACATCACTACTTAA	ASRARFNTLAIIPPRCNITT*	-1.409	0.000125	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25350	CCAMVRRLFHWLRLSSFIGH			20	SLAY-screened peptide P3700			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTGTGCTATGGTCCGGCGGCTGTTCCACTGGCTTCGCCTCTCCTCGTTTATCGGTCACTAA	CCAMVRRLFHWLRLSSFIGH*	-1.409	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25351	LIYLLIFIFPQHITYGLLDD			20	SLAY-screened peptide P3701			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATTTATCTTTTGATTTTCATTTTTCCCCAGCACATTACTTACGGGCTTCTCGATGACTAA	LIYLLIFIFPQHITYGLLDD*	-1.408	0.014022	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25352	VTNPLMPLARRSITSSPYVG			20	SLAY-screened peptide P3702			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCACTAACCCCCTCATGCCCCTCGCCAGGCGCTCCATTACGAGCTCCCCTTACGTCGGCTAA	VTNPLMPLARRSITSSPYVG*	-1.408	0.000158	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25353	TKRPILAPLYGFDDVAPFST			20	SLAY-screened peptide P3703			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAAGCGCCCTATCCTTGCCCCGCTCTATGGGTTCGACGACGTCGCCCCTTTCAGCACCTAA	TKRPILAPLYGFDDVAPFST*	-1.408	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25354	QSVPPSYLLHFISPLGPCFV			20	SLAY-screened peptide P3704			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTCTGTCCCCCCTTCTTATCTCCTGCATTTTATTTCCCCTTTGGGGCCTTGTTTTGTGTAA	QSVPPSYLLHFISPLGPCFV*	-1.408	0.003129	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25355	CLWDPVPITGYLAVNALRTF			20	SLAY-screened peptide P3705			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTTTGGGACCCTGTTCCGATCACGGGCTACCTTGCGGTTAACGCTTTGCGTACGTTTTAA	CLWDPVPITGYLAVNALRTF*	-1.408	7.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25356	LNVRRHNFCPITVPTYNIMF			20	SLAY-screened peptide P3706			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAACGTTCGTCGGCATAACTTCTGCCCCATCACTGTGCCTACTTATAACATTATGTTTTAA	LNVRRHNFCPITVPTYNIMF*	-1.408	0.000585	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25357	SL			2	SLAY-screened peptide P3707			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCTGTAGGTCGCTCATAAGTGTCGTGAGGTGAGCACGCTTTTCTGAAGAAGATCCCGTAAC	SL*VAHKCREVSTLF*RRSRN	-1.407	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25358	SSYTHPFTRPCAKDMLMLLT			20	SLAY-screened peptide P3708			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTCCTACACCCACCCTTTCACGCGCCCGTGCGCCAAGGACATGCTTATGCTGCTTACTTAA	SSYTHPFTRPCAKDMLMLLT*	-1.407	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25359	FPHHMYDVRLIQFPPNSTSR			20	SLAY-screened peptide P3709			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCCTCATCATATGTATGATGTTCGCCTTATCCAGTTTCCTCCCAATTCTACCTCCCGTTAA	FPHHMYDVRLIQFPPNSTSR*	-1.407	0.021074	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25360	SMLLVRLNHRVHFSHAYKRF			20	SLAY-screened peptide P3710			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCATGCTTCTTGTTCGCCTTAATCATCGTGTCCACTTCTCGCACGCTTACAAGCGTTTCTAA	SMLLVRLNHRVHFSHAYKRF*	-1.407	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25361	PNCLHLLQNSVCTRWLTSSR			20	SLAY-screened peptide P3711			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAATTGTCTCCACTTGCTTCAGAACAGTGTTTGCACCAGGTGGCTCACTTCCAGTAGGTAA	PNCLHLLQNSVCTRWLTSSR*	-1.407	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25362	RWCDVPPLVDYEIAPAILPS			20	SLAY-screened peptide P3712			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGGTGTGATGTGCCGCCCCTCGTCGATTATGAGATCGCTCCTGCTATTTTGCCGAGCTAA	RWCDVPPLVDYEIAPAILPS*	-1.407	0.013488	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25363	SYDQDMFINWHPCHDSHYHL			20	SLAY-screened peptide P3713			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTATGACCAGGACATGTTTATTAATTGGCATCCTTGCCACGATAGTCATTATCACCTGTAA	SYDQDMFINWHPCHDSHYHL*	-1.407	0.00636	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25364	TLQYSPHVPDRTWYVHN			17	SLAY-screened peptide P3714			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTCCAGTATTCCCCCCATGTCCCGGATAGGACTTGGTATGTTCACAATTAGTTTTGCTAA	TLQYSPHVPDRTWYVHN*FC*	-1.407	0.000414	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25365	INGFAERRSVSLPLLIRGTFN			21	SLAY-screened peptide P3715			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAACGGTTTTGCGGAGAGGCGTTCGGTTAGTCTTCCGCTACTGATCCGTGGCACTTTTAAC	INGFAERRSVSLPLLIRGTFN	-1.406	0.025627	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25366	IVS			3	SLAY-screened peptide P3716			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGTTTCTTAGTCGTACCCGGGCCGGGTTACGGTCCTTAATAGCTACAACACTGGTTTCTAA	IVS*SYPGRVTVLNSYNTGF*	-1.406	0.016867	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25367	YARNDVLCQTSISMMIITPSN			21	SLAY-screened peptide P3717			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGCGCGGAACGATGTCCTGTGTCAGACGAGCATCAGCATGATGATCATAACCCCAAGTAAC	YARNDVLCQTSISMMIITPSN	-1.406	0.000231	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25368	STHPNTGSNIEPPNFRSSDS			20	SLAY-screened peptide P3718			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGACTCACCCTAATACCGGCTCGAACATCGAGCCCCCCAACTTCCGCTCCTCCGACTCTTAA	STHPNTGSNIEPPNFRSSDS*	-1.406	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25369	IFATTSRFQSHSLLALKILF			20	SLAY-screened peptide P3719			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTTTGCCACCACTAGCCGGTTTCAGTCTCACTCTCTCCTGGCCCTCAAGATTCTGTTCTAA	IFATTSRFQSHSLLALKILF*	-1.406	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25370	PFTRNHGPRCLRLRDFCSCH			20	SLAY-screened peptide P3720			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTCACGAGGAACCACGGGCCCCGTTGCTTGCGGCTTCGTGATTTCTGTTCTTGCCACTAA	PFTRNHGPRCLRLRDFCSCH*	-1.405	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25371	LRPTYTSPAIYRPYNLYSHS			20	SLAY-screened peptide P3721			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCGCCCCACCTACACGAGTCCTGCCATTTACAGGCCTTACAACCTCTACAGTCACAGTTAA	LRPTYTSPAIYRPYNLYSHS*	-1.405	0.001397	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25372	LFIILARWLNHYDKSKGITP			20	SLAY-screened peptide P3722			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTTTATTATCCTCGCCAGGTGGCTCAATCACTACGATAAGTCTAAGGGCATCACTCCTTAA	LFIILARWLNHYDKSKGITP*	-1.405	0.000619	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25373	HPSRQPTSCLNTSDFLYVYL			20	SLAY-screened peptide P3723			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCCTCCCGTCAGCCTACTTCGTGCCTTAACACCAGCGACTTTCTTTACGTCTACCTCTAA	HPSRQPTSCLNTSDFLYVYL*	-1.405	0.000139	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25374	PGSGSH			6	SLAY-screened peptide P3724			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGGAGTGGGTCGCATTAGCGTCATGTTATGTGGCTGTGGTGTCCCTCTCGCACCCTCTAA	PGSGSH*RHVMWLWCPSRTL*	-1.405	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25375	TRGINVVSAHTHVPHNSFAV			20	SLAY-screened peptide P3725			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCGGGGTATTAACGTGGTCAGCGCCCATACCCATGTCCCCCACAATTCGTTCGCCGTCTAA	TRGINVVSAHTHVPHNSFAV*	-1.405	0.040434	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25376	APIANPCRIFYVPNIWLPYV			20	SLAY-screened peptide P3726			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCCATCGCGAATCCTTGCAGGATTTTCTACGTGCCCAATATTTGGCTGCCGTACGTGTAA	APIANPCRIFYVPNIWLPYV*	-1.405	3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25377	SYLTCPPSVFPQWSV			15	SLAY-screened peptide P3727			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTACCTCACCTGCCCTCCTTCCGTTTTCCCCCAGTGGAGCGTGTAGCACCATTGCACCTAA	SYLTCPPSVFPQWSV*HHCT*	-1.405	0.034489	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25378	YGLVSFTHPGQHTDYRVMLH			20	SLAY-screened peptide P3728			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGGTCTGGTTAGTTTCACGCATCCTGGTCAGCACACGGATTACCGTGTTATGCTCCATTAA	YGLVSFTHPGQHTDYRVMLH*	-1.405	0.017657	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25379	LWKIPIPKAQYSRRIHCLGT			20	SLAY-screened peptide P3729			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTGGAAGATTCCGATTCCGAAGGCCCAGTACTCCCGTAGGATCCATTGTCTTGGCACGTAA	LWKIPIPKAQYSRRIHCLGT*	-1.404	0.000229	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25380	RPITAISLSVLVLGSIRLRFN			21	SLAY-screened peptide P3730			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCCATAACCGCTATCTCACTAAGCGTACTCGTGCTAGGGTCAATTAGACTTCGGTTTAAC	RPITAISLSVLVLGSIRLRFN	-1.404	1.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25381	NCTANVLVGII			11	SLAY-screened peptide P3731			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTGTACTGCCAATGTTCTTGTTGGCATTATCTAGATGATTATGACTGAGAAGGCTCCTTAA	NCTANVLVGII*MIMTEKAP*	-1.404	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25382	TCYHNLHPEFRYPTLDFSMI			20	SLAY-screened peptide P3732			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTGTTATCACAATTTGCACCCTGAGTTCCGGTACCCCACTCTCGATTTCAGTATGATCTAA	TCYHNLHPEFRYPTLDFSMI*	-1.404	0.025698	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25383	FPRLGYSYDLVSPCYLPLLN			20	SLAY-screened peptide P3733			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCCTCGGCTTGGCTATTCCTATGACCTCGTTAGCCCTTGTTACTTGCCCTTGTTGAACTAA	FPRLGYSYDLVSPCYLPLLN*	-1.404	0.015514	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25384	RAP			3	SLAY-screened peptide P3734			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGCGCCCTGATGCCGACGCCCAAGCCGTGCGAGATGAAGCACTCGTGTTTCCCGCTTTAAC	RAP*CRRPSRAR*STRVSRFN	-1.404	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25385	PTVFFTARPSFQSDNHTAFW			20	SLAY-screened peptide P3735			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACTGTCTTCTTCACCGCCCGGCCCTCTTTTCAGAGTGACAATCATACCGCTTTCTGGTAA	PTVFFTARPSFQSDNHTAFW*	-1.404	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25386	HA			2	SLAY-screened peptide P3736			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCTTAGCAGCAGAACATTCATAATCCGCCTACGACGTGGGTTTATAGTAGCCCCAAGTAA	HA*QQNIHNPPTTWVYSSPK*	-1.404	0.003106	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25387	SDISPTRPWLYNNYECWAPL			20	SLAY-screened peptide P3737			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGACATTTCTCCTACGCGTCCCTGGCTCTACAACAATTACGAGTGCTGGGCCCCGCTTTAA	SDISPTRPWLYNNYECWAPL*	-1.403	0.027406	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25388	RLYRHPTGRQSRPLGCFATF			20	SLAY-screened peptide P3738			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTTTATCGCCATCCGACTGGCCGTCAGAGTCGTCCGCTGGGTTGTTTCGCCACGTTCTAA	RLYRHPTGRQSRPLGCFATF*	-1.403	0.016425	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25389	FSVSHTYSDHVGTNLHISGL			20	SLAY-screened peptide P3739			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTAGCGTCAGCCACACTTATAGTGACCATGTGGGCACTAATCTGCACATCTCCGGCCTCTAA	FSVSHTYSDHVGTNLHISGL*	-1.403	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25390	HSGSPLSCDAPNFYPYFWHV			20	SLAY-screened peptide P3740			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTCCGGCTCGCCTCTCAGCTGTGATGCGCCCAATTTTTATCCTTACTTTTGGCACGTCTAA	HSGSPLSCDAPNFYPYFWHV*	-1.402	0.038027	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25391	SRKHTCFIADCSQCSLQFVN			20	SLAY-screened peptide P3741			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCGGAAGCACACGTGCTTCATTGCGGACTGCAGCCAGTGCAGCCTCCAGTTCGTTAATTAA	SRKHTCFIADCSQCSLQFVN*	-1.402	0.000158	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25392	SVMYTCNTYTTWAGRPTVVT			20	SLAY-screened peptide P3742			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGTCATGTATACTTGCAATACGTACACTACTTGGGCCGGCCGTCCCACTGTTGTGACCTAA	SVMYTCNTYTTWAGRPTVVT*	-1.402	0.04727	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25393	PIHKSQNLHGYLLFIIRAPP			20	SLAY-screened peptide P3743			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATCCATAAGTCCCAGAATCTCCACGGCTACTTGCTCTTCATTATCAGGGCCCCCCCTTAA	PIHKSQNLHGYLLFIIRAPP*	-1.402	0.0163	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25394	PSLGLHHFHTIAQNLVNATK			20	SLAY-screened peptide P3744			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGCCTGGGCCTGCATCATTTTCACACCATCGCGCAGAATTTGGTGAATGCCACTAAGTAA	PSLGLHHFHTIAQNLVNATK*	-1.402	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25395	RPTVKADCLLFLNFFNVHNN			20	SLAY-screened peptide P3745			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCTACCGTTAAGGCGGACTGTCTGCTTTTTCTCAATTTCTTCAATGTTCATAATAATTAA	RPTVKADCLLFLNFFNVHNN*	-1.401	0.011903	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25396	VLRNTLHCCSIVIRSSSGHW			20	SLAY-screened peptide P3746			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCTTCGTAACACCCTTCACTGCTGCTCGATTGTTATTCGCTCGTCCTCGGGTCATTGGTAA	VLRNTLHCCSIVIRSSSGHW*	-1.401	0.00074	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25397	AVPLLHGVPLFWYF			14	SLAY-screened peptide P3747			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGTTCCTCTCCTGCACGGTGTCCCTCTCTTCTGGTATTTCTAGTTGACCTCTGCTTGGTAA	AVPLLHGVPLFWYF*LTSAW*	-1.401	0.040579	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25398	FLPVRYILGRPIL			13	SLAY-screened peptide P3748			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTTGCCCGTGCGCTACATTCTGGGCCGCCCTATTCTTTAGCGTTAGAGTCAGACCACGTAA	FLPVRYILGRPIL*R*SQTT*	-1.401	0.010932	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25399	QLETRPSYNFTILPAYTKVN			20	SLAY-screened peptide P3749			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCTTGAGACTCGTCCCAGCTATAATTTTACTATTCTCCCTGCCTACACTAAGGTTAATTAA	QLETRPSYNFTILPAYTKVN*	-1.401	0.005856	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25400	SPSTNFCNVLRLDTLNVNRS			20	SLAY-screened peptide P3750			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCCCTCCACGAACTTCTGCAATGTTCTCCGCCTGGACACCTTGAATGTGAACCGTAGTTAA	SPSTNFCNVLRLDTLNVNRS*	-1.401	0.00014	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25401	ACTPRSWGPGPLTPTSTI			18	SLAY-screened peptide P3751			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTGTACCCCCAGATCCTGGGGGCCGGGACCTCTTACTCCCACTTCCACGATATGACTTAAC	ACTPRSWGPGPLTPTSTI*LN	-1.4	0.036448	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25402	TLHHCNDSSQHYASYYLYRA			20	SLAY-screened peptide P3752			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTTCATCACTGTAATGACTCTTCCCAGCACTACGCTAGCTATTATTTGTACCGTGCCTAA	TLHHCNDSSQHYASYYLYRA*	-1.4	0.000508	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25403	NHLCGPLDYSDVFSDLPDDW			20	SLAY-screened peptide P3753			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCATCTTTGCGGTCCGCTTGATTACAGTGACGTCTTCAGTGATTTGCCTGACGACTGGTAA	NHLCGPLDYSDVFSDLPDDW*	-1.4	0.008096	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25404	PLFGRAGLEIHITTFSYSTM			20	SLAY-screened peptide P3754			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTCTTTGGCCGCGCCGGGCTGGAGATCCACATTACTACTTTCTCCTACTCTACCATGTAA	PLFGRAGLEIHITTFSYSTM*	-1.4	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25405	LSYNSTHYNQHVAYTTFYCT			20	SLAY-screened peptide P3755			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCTTACAACTCTACTCACTATAATCAGCATGTTGCTTATACGACCTTTTATTGCACGTAA	LSYNSTHYNQHVAYTTFYCT*	-1.4	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25406	QPSTVITRLPMRRYHSLHVA			20	SLAY-screened peptide P3756			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCGTCTACTGTCATCACGCGTCTCCCCATGCGGCGCTATCACAGTCTCCATGTCGCCTAA	QPSTVITRLPMRRYHSLHVA*	-1.4	0.00206	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25407	AALRHTPWHTSRTHQDRGFS			20	SLAY-screened peptide P3757			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCGCTTCGTCATACCCCGTGGCATACCTCTCGTACGCATCAGGACCGCGGGTTCTCCTAA	AALRHTPWHTSRTHQDRGFS*	-1.399	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25408	HHSLGFINRYL			11	SLAY-screened peptide P3758			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCATAGCCTTGGCTTTATTAACCGTTATCTTTAGGATAGCTGCTTTTCCAATAACCTGTAA	HHSLGFINRYL*DSCFSNNL*	-1.399	0.001283	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25409	NSPEPVFLDPRCPGWPYRIV			20	SLAY-screened peptide P3759			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACAGTCCGGAGCCCGTCTTTTTGGACCCCCGGTGTCCTGGTTGGCCGTACCGCATTGTGTAA	NSPEPVFLDPRCPGWPYRIV*	-1.398	0.011909	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25410	TPAIYADSIARSRAHHDYCS			20	SLAY-screened peptide P3760			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCCCGCGATCTACGCTGATTCCATTGCGCGGAGTAGGGCGCATCATGACTATTGTTCCTAA	TPAIYADSIARSRAHHDYCS*	-1.398	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25411	HLVKFFHGNFAGF			13	SLAY-screened peptide P3761			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCTGGTCAAGTTTTTTCACGGCAATTTCGCTGGGTTCTAGGATAGGCATTCTGACATTTAA	HLVKFFHGNFAGF*DRHSDI*	-1.398	0.000168	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25412	QPITYGYHEVAAPIYAKMDC			20	SLAY-screened peptide P3762			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCCATTACCTATGGGTATCATGAGGTGGCCGCTCCGATTTACGCTAAGATGGATTGTTAA	QPITYGYHEVAAPIYAKMDC*	-1.398	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25413	PHYSLGHSSKYTRSTIMYFL			20	SLAY-screened peptide P3763			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCACTACTCTCTCGGTCACTCGAGTAAGTACACCCGGAGTACTATTATGTATTTCCTTTAA	PHYSLGHSSKYTRSTIMYFL*	-1.398	5.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25414	CHVPNSIIWSRGYITQYYCA			20	SLAY-screened peptide P3764			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCACGTCCCGAACTCCATCATTTGGTCCCGTGGCTATATTACCCAGTATTATTGCGCGTAA	CHVPNSIIWSRGYITQYYCA*	-1.398	0.030375	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25415	TGRFALPPDQPVICWFHFNH			20	SLAY-screened peptide P3765			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGGCCGGTTTGCCTTGCCCCCGGATCAGCCTGTTATCTGCTGGTTCCATTTTAACCATTAA	TGRFALPPDQPVICWFHFNH*	-1.398	0.023172	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25416	SITGHYSDSSVSGHKGDNSGN			21	SLAY-screened peptide P3766			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGATTACCGGCCACTACTCCGATTCGTCGGTTAGTGGTCACAAGGGCGATAACTCAGGTAAC	SITGHYSDSSVSGHKGDNSGN	-1.397	0.018272	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25417	LHDPSARFIPHRFLFRDAKN			20	SLAY-screened peptide P3767			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCACGATCCCAGTGCGCGCTTTATCCCGCACCGCTTTCTCTTCCGGGACGCGAAGAACTAA	LHDPSARFIPHRFLFRDAKN*	-1.397	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25418	PKRTCLNVVRTIMRAYFRRH			20	SLAY-screened peptide P3768			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAAGCGTACGTGTCTGAACGTGGTTCGGACGATCATGCGTGCTTATTTCAGGAGGCATTAA	PKRTCLNVVRTIMRAYFRRH*	-1.396	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25419	PYHPIALEVARTFVKSRPP			19	SLAY-screened peptide P3769			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTATCATCCCATCGCCCTTGAGGTGGCCCGCACTTTTGTTAAGTCTCGTCCTCCGTAGTAA	PYHPIALEVARTFVKSRPP**	-1.396	0.000265	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25420	PRTLGPGSPFFSRLVQDYVTE			21	SLAY-screened peptide P3770			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGTACCCTCGGACCCGGCTCACCGTTCTTCTCCCGACTCGTACAAGACTATGTAACTGAG	PRTLGPGSPFFSRLVQDYVTE	-1.396	8.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25421	PVNMFMNALERLVRRYIDRD			20	SLAY-screened peptide P3771			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGTCAATATGTTTATGAATGCTCTGGAGCGGCTCGTCAGGCGGTACATCGATCGCGATTAA	PVNMFMNALERLVRRYIDRD*	-1.395	0.015914	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25422	PFEWDPVSSITYAHVSPHDT			20	SLAY-screened peptide P3772			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTTGAGTGGGATCCCGTGTCGTCGATTACCTATGCTCACGTCTCTCCGCATGATACTTAA	PFEWDPVSSITYAHVSPHDT*	-1.395	0.005198	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25423	FLFSGPIGQNCFHQRNYSHV			20	SLAY-screened peptide P3773			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTTGTTCTCCGGCCCGATTGGGCAGAACTGCTTCCACCAGCGTAACTATTCCCACGTTTAA	FLFSGPIGQNCFHQRNYSHV*	-1.395	0.001391	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25424	GQSVNLHYLRAYIDSPYNA			19	SLAY-screened peptide P3774			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCAGAGCGTCAACTTGCACTATCTCCGTGCGTACATCGACTCCCCCTACAACGCTTAACTG	GQSVNLHYLRAYIDSPYNA*L	-1.395	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25425	SRYWACHDAGLIVCYFWCIR			20	SLAY-screened peptide P3775			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCGTTACTGGGCCTGTCATGATGCTGGTCTTATTGTTTGCTATTTTTGGTGCATCAGGTAA	SRYWACHDAGLIVCYFWCIR*	-1.395	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25426	LVPSDPVPPYPYYQPLHLVY			20	SLAY-screened peptide P3776			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGTCCCCTCGGACCCGGTTCCGCCCTACCCCTATTATCAGCCTCTCCATCTCGTTTACTAA	LVPSDPVPPYPYYQPLHLVY*	-1.395	0.003958	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25427	PAELTRQNHGYDWNDYTFNN			20	SLAY-screened peptide P3777			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCTGAGCTCACTAGGCAGAATCACGGTTATGATTGGAATGACTATACTTTCAATAACTAA	PAELTRQNHGYDWNDYTFNN*	-1.395	0.00337	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25428	SALSFSYPNPSPSTRIHCFQ			20	SLAY-screened peptide P3778			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGCTTTGAGCTTTTCCTATCCTAATCCTAGCCCGTCTACGCGCATCCATTGCTTTCAGTAA	SALSFSYPNPSPSTRIHCFQ*	-1.395	0.026815	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25429	RPQLVFVMLHLHLLVRRYCH			20	SLAY-screened peptide P3779			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCCCAGCTGGTTTTTGTCATGTTGCATCTTCACCTCCTCGTTCGCCGTTATTGTCATTAA	RPQLVFVMLHLHLLVRRYCH*	-1.394	0.03863	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25430	TSKLNNRLLLLLRKLCLSLV			20	SLAY-screened peptide P3780			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCGAAGCTGAACAATCGCCTCTTGCTTCTGCTCCGTAAGCTCTGCCTGTCCCTCGTGTAA	TSKLNNRLLLLLRKLCLSLV*	-1.394	0.001184	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25431	PRLYCGTIDHTLLSFAWDRP			20	SLAY-screened peptide P3781			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGCCTGTACTGCGGGACCATTGATCACACTCTGCTCTCTTTTGCTTGGGACAGGCCTTAA	PRLYCGTIDHTLLSFAWDRP*	-1.394	0.000185	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25432	PDPAKHRHTLTLYRTGGALTN			21	SLAY-screened peptide P3782			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGACCCGGCTAAGCATCGGCATACCTTGACCCTTTATCGCACCGGCGGAGCGCTTACTAAC	PDPAKHRHTLTLYRTGGALTN	-1.394	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25433	RSPNHHRLIPGLDNISLDTR			20	SLAY-screened peptide P3783			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTCCCCTAACCACCATCGGCTTATTCCCGGGCTTGACAATATTTCGCTCGATACTCGCTAA	RSPNHHRLIPGLDNISLDTR*	-1.393	0.008148	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25434	RSHTKCRRLTQDPHSSGCIE			20	SLAY-screened peptide P3784			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCCCATACCAAGTGCCGTCGTCTCACCCAGGACCCTCATTCCAGTGGGTGCATTGAGTAA	RSHTKCRRLTQDPHSSGCIE*	-1.393	0.00271	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25435	PRFSSPTSPKECTPCTSNHN			20	SLAY-screened peptide P3785			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGGTTTTCTTCTCCCACGTCCCCTAAGGAGTGTACCCCGTGTACTTCCAACCACAACTAA	PRFSSPTSPKECTPCTSNHN*	-1.393	0.004654	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25436	VPNSHYGAISSGFLITGRVM			20	SLAY-screened peptide P3786			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCCCAACTCCCACTATGGTGCCATTTCCTCTGGGTTTTTGATCACGGGGAGGGTCATGTAA	VPNSHYGAISSGFLITGRVM*	-1.393	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25437	DSNFSDYSLNNCWLYYRLTH			20	SLAY-screened peptide P3787			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTCTAATTTCTCGGATTATAGTCTCAATAACTGCTGGCTTTATTACCGCCTCACGCACTAA	DSNFSDYSLNNCWLYYRLTH*	-1.392	0.027743	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25438	TTTAAPASRTCNGMILCNGTT			21	SLAY-screened peptide P3788			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTACCACCGCCGCGCCCGCCTCGCGTACCTGCAACGGCATGATCTTGTGCAACGGTACCACT	TTTAAPASRTCNGMILCNGTT	-1.392	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25439	YHSSTSTCPRGDPIATPTTR			20	SLAY-screened peptide P3789			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCACTCCAGTACGTCTACCTGCCCCCGGGGCGATCCTATTGCTACGCCTACCACCAGGTAA	YHSSTSTCPRGDPIATPTTR*	-1.392	0.003568	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25440	PSTNGNTVLDHVLYIYGFGS			20	SLAY-screened peptide P3790			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCCACGAATGGCAACACCGTGCTTGACCACGTCCTTTATATTTATGGCTTTGGCTCTTAA	PSTNGNTVLDHVLYIYGFGS*	-1.392	0.01344	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25441	QPPPLAQAITSRSHTKVYRV			20	SLAY-screened peptide P3791			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCCCCTCCCTTGGCTCAGGCCATTACCTCGCGCTCGCACACCAAGGTTTATCGCGTTTAA	QPPPLAQAITSRSHTKVYRV*	-1.392	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25442	TPNHQIDVFRRYFTCVSTHG			20	SLAY-screened peptide P3792			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCCGAATCATCAGATTGACGTCTTCCGTCGGTACTTCACTTGCGTTAGTACTCATGGCTAA	TPNHQIDVFRRYFTCVSTHG*	-1.392	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25443	SDCPDALYKCNRVKNHVAMM			20	SLAY-screened peptide P3793			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGACTGCCCTGACGCGCTGTATAAGTGTAACAGGGTCAAGAACCACGTCGCTATGATGTAA	SDCPDALYKCNRVKNHVAMM*	-1.391	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25444	SNPFYPLCCCRWTIGCCQSI			20	SLAY-screened peptide P3794			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTAATCCGTTCTACCCTCTTTGTTGTTGCCGCTGGACTATCGGTTGTTGTCAGAGTATTTAA	SNPFYPLCCCRWTIGCCQSI*	-1.391	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25445	NGSFLQQWLPLSLFIC			16	SLAY-screened peptide P3795			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGGGTCCTTCCTCCAGCAGTGGCTTCCTTTGAGCCTTTTTATTTGTTAGCGCTTCACTTAA	NGSFLQQWLPLSLFIC*RFT*	-1.391	0.029315	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25446	MLAILFTLNARRPFHAPVVI			20	SLAY-screened peptide P3796			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCTTGCCATCCTGTTTACCCTTAATGCGCGCCGCCCGTTTCACGCCCCCGTGGTCATTTAA	MLAILFTLNARRPFHAPVVI*	-1.39	0.000875	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25447	GTQTYTICSFHPHDSNILVI			20	SLAY-screened peptide P3797			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTACGCAGACCTATACGATTTGTTCTTTCCACCCCCATGACTCGAACATCCTGGTCATTTAA	GTQTYTICSFHPHDSNILVI*	-1.39	0.000513	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25448	PMPASCLYT			9	SLAY-screened peptide P3798			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATGCCTGCCTCTTGCCTCTATACTTAGGAGTCCAAGGATATGAACGCGGCTCTGTTCTAA	PMPASCLYT*ESKDMNAALF*	-1.39	0.048958	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25449	SHKDYNSCNFFKDTVVNYFN			20	SLAY-screened peptide P3799			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCATAAGGACTATAACTCTTGTAATTTCTTTAAGGACACGGTGGTCAATTATTTTAATTAA	SHKDYNSCNFFKDTVVNYFN*	-1.39	0.001045	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25450	RSQYGRFVRHLGKPSIAHVT			20	SLAY-screened peptide P3800			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAGCCAGTACGGTCGTTTTGTCCGCCACCTTGGCAAGCCCTCGATCGCTCATGTTACTTAA	RSQYGRFVRHLGKPSIAHVT*	-1.39	0.048522	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25451	NIVTDPSFSCPYFTSLN			17	SLAY-screened peptide P3801			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACATTGTCACGGACCCGAGCTTTTCCTGTCCGTACTTTACTTCCCTGAATTAGCCGGCCTAA	NIVTDPSFSCPYFTSLN*PA*	-1.39	0.001562	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25452	AFTSPHVICYRSVTLTMRPTN			21	SLAY-screened peptide P3802			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTTCACCAGCCCGCACGTCATCTGCTACCGTAGTGTTACCCTGACCATGAGACCCACTAAC	AFTSPHVICYRSVTLTMRPTN	-1.39	4.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25453	TASPHGPTA			9	SLAY-screened peptide P3803			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGCTAGCCCGCACGGCCCTACGGCCTAGCATTTTCTCATCGTGTTTGATCACACCAACTAA	TASPHGPTA*HFLIVFDHTN*	-1.389	8.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25454	SLAQP			5	SLAY-screened peptide P3804			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTTGGCGCAGCCGTAGCCTAATATCCTGCACTTTAGTCATACTTACGGTACCGTCAGCTAA	SLAQP*PNILHFSHTYGTVS*	-1.388	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25455	YSRISPRRNTCSFYSSLTST			20	SLAY-screened peptide P3805			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTCCCGTATTTCCCCGCGTCGCAATACGTGCAGCTTTTACTCTTCCCTTACTTCTACTTAA	YSRISPRRNTCSFYSSLTST*	-1.388	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25456	ANNDACPTPLYFGPPPSFYL			20	SLAY-screened peptide P3806			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAACAATGACGCGTGCCCTACTCCCCTCTATTTTGGTCCTCCTCCTTCCTTCTACCTCTAA	ANNDACPTPLYFGPPPSFYL*	-1.388	0.024065	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25457	THVVHWYGYY			10	SLAY-screened peptide P3807			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCACGTTGTTCATTGGTATGGTTATTATTAGGCTGCTCAGTATTCCAGCTTTTGGGCTTAA	THVVHWYGYY*AAQYSSFWA*	-1.388	0.015255	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25458	CMSKKRNTFDTGYMIPQSII			20	SLAY-screened peptide P3808			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTATGTCTAAGAAGCGTAACACCTTTGACACTGGCTACATGATCCCTCAGAGTATTATTTAA	CMSKKRNTFDTGYMIPQSII*	-1.387	0.035789	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25459	YFRKLYQRRNNRWSNLFFN			19	SLAY-screened peptide P3809			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGGTACTTCAGGAAGCTTTATCAGCGCCGCAACAATCGGTGGTCCAATCTTTTTTTCAACTAACTG	YFRKLYQRRNNRWSNLFFN*L	-1.387	0.031918	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25460	APWGNATL			8	SLAY-screened peptide P3810			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCCCTGGGGGAATGCCACCCTTTAGACTACCAGGCCCCTGTCTGATTACGCGTAGCGTTAA	APWGNATL*TTRPLSDYA*R*	-1.387	4.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25461	DGIVSVTPKGDNLRLAAGPP			20	SLAY-screened peptide P3811			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGGCATCGTTTCGGTGACGCCCAAGGGTGATAATCTCAGGTTGGCCGCCGGCCCGCCTTAA	DGIVSVTPKGDNLRLAAGPP*	-1.387	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25462	LVIEPLMIWRL			11	SLAY-screened peptide P3812			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGTCATCGAGCCTCTGATGATCTGGCGTCTTTAGATTACGTTCCTGCCTTGTATTATCTAA	LVIEPLMIWRL*ITFLPCII*	-1.387	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25463	DVLTILLFRCCIVLRVSLSFN			21	SLAY-screened peptide P3813			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGTTCTTACAATACTATTATTTAGGTGCTGCATAGTACTAAGGGTCTCATTGTCTTTTAAC	DVLTILLFRCCIVLRVSLSFN	-1.387	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25464	HSCSEPIFCSNASALGICPLN			21	SLAY-screened peptide P3814			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAGCTGTTCCGAGCCGATCTTTTGTTCTAATGCATCTGCCCTAGGCATATGTCCTCTTAAC	HSCSEPIFCSNASALGICPLN	-1.386	0.000177	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25465	EASLGRLHSVAAQA			14	SLAY-screened peptide P3815			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGCCAGTCTCGGTCGTTTGCACAGCGTCGCTGCTCAGGCCTAGCACCCCACTCCGTTGTAA	EASLGRLHSVAAQA*HPTPL*	-1.386	0.004529	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25466	IRLFHNARSRPRSWNNYLQM			20	SLAY-screened peptide P3816			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCGGCTTTTTCATAACGCTCGTAGCCGCCCCCGGAGTTGGAATAATTACCTCCAGATGTAA	IRLFHNARSRPRSWNNYLQM*	-1.386	0.007758	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25467	RRLPSGPPPVQSVYSHAV			18	SLAY-screened peptide P3817			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGTCTCCCTTCCGGGCCCCCTCCAGTTCAGTCCGTTTATTCCCATGCTGTGTAGGTAACT	RRLPSGPPPVQSVYSHAV*VT	-1.386	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25468	SRTPTSARRQLKNMNKEHLF			20	SLAY-screened peptide P3818			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCGCACGCCGACCTCTGCCCGGCGTCAGCTGAAGAATATGAATAAGGAGCACCTCTTCTAA	SRTPTSARRQLKNMNKEHLF*	-1.385	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25469	PRNNTAPRVSMLSILLTVTAN			21	SLAY-screened peptide P3819			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGGAACAATACAGCCCCTCGTGTTTCTATGTTGTCCATCCTTCTTACGGTAACCGCTAAC	PRNNTAPRVSMLSILLTVTAN	-1.385	0.017891	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25470	CVLQHAFYTL			10	SLAY-screened peptide P3820			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGTGTTGCAGCACGCCTTTTATACTCTCTAGTTCCTTACTCCTATTAGTTTTCATAATTAA	CVLQHAFYTL*FLTPISFHN*	-1.385	0.000655	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25471	TFVCRVPCRITPSLVCRIFSN			21	SLAY-screened peptide P3821			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTTCGTATGTAGGGTGCCCTGTCGTATTACTCCAAGCCTTGTATGCCGAATATTTTCTAAC	TFVCRVPCRITPSLVCRIFSN	-1.385	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25472	CPFGYPFNRCRACTLPFMAV			20	SLAY-screened peptide P3822			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCTTTCGGTTACCCGTTTAATCGGTGCCGTGCGTGCACTTTGCCTTTCATGGCCGTCTAA	CPFGYPFNRCRACTLPFMAV*	-1.385	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25473	HT			2	SLAY-screened peptide P3823			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACGTAGTCCATCTATTATTCTTATTCCCACCCCCGTGATTCTGTGGGGCGGCCCCACTAA	HT*SIYYSYSHPRDSVGRPH*	-1.384	0.04543	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25474	DRRNGLYGPCTSPLLMFGYT			20	SLAY-screened peptide P3824			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATAGGCGCAATGGCCTTTACGGTCCCTGCACGTCCCCCCTCCTGATGTTCGGCTATACCTAA	DRRNGLYGPCTSPLLMFGYT*	-1.384	0.043313	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25475	QIVPRAAILHYLTFRTHPSR			20	SLAY-screened peptide P3825			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGATCGTCCCTCGGGCCGCTATCCTTCATTACCTGACTTTCCGTACCCACCCTAGTAGGTAA	QIVPRAAILHYLTFRTHPSR*	-1.384	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25476	ESCSLYDYLAFLLM			14	SLAY-screened peptide P3826			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGAGTTGTTCCTTGTATGACTATCTGGCCTTCCTTCTTATGTAGATTATGTATTTTGCCTAA	ESCSLYDYLAFLLM*IMYFA*	-1.384	0.045951	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25477	CPLSSMNASYPTYSIFTI			18	SLAY-screened peptide P3827			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCTCTCTCGTCTATGAATGCTAGTTACCCCACTTATTCCATTTTTACGATCTAGGTCTAA	CPLSSMNASYPTYSIFTI*V*	-1.384	0.000667	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25478	PWCSKYCWSCPLYNLGFSP			19	SLAY-screened peptide P3828			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGGTGTTCTAAGTACTGCTGGTCGTGCCCCCTTTATAACTTGGGGTTTAGCCCGTAACTG	PWCSKYCWSCPLYNLGFSP*L	-1.384	9.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25479	PRPLVCALSVSQMYQRRHSS			20	SLAY-screened peptide P3829			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGGCCTCTGGTCTGTGCGCTCTCCGTCTCTCAGATGTACCAGCGCCGCCATTCCTCTTAA	PRPLVCALSVSQMYQRRHSS*	-1.384	0.000825	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25480	PMFLLHPCPYFPISKDMQFW			20	SLAY-screened peptide P3830			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATGTTCCTGTTGCACCCGTGTCCCTACTTTCCCATTTCCAAGGACATGCAGTTCTGGTAA	PMFLLHPCPYFPISKDMQFW*	-1.383	0.003337	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25481	DDLTGTGRLFHCHI			14	SLAY-screened peptide P3831			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGACCTGACCGGCACGGGTCGCCTTTTCCATTGTCACATCTAGGTGTAACTGAGTAAGTCG	DDLTGTGRLFHCHI*V*LSKS	-1.383	0.026413	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25482	HDSTLCSHGPYYLHYVSSFL			20	SLAY-screened peptide P3832			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGACAGCACTCTGTGCAGTCATGGGCCGTACTACCTCCATTACGTTTCCTCTTTCTTGTAA	HDSTLCSHGPYYLHYVSSFL*	-1.383	0.002964	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25483	IGQHSPHPICPRDTSD			16	SLAY-screened peptide P3833			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGGTCAGCATTCCCCTCATCCCATTTGTCCGAGGGATACGTCCGATTAGTGTGGCGATTAA	IGQHSPHPICPRDTSD*CGD*	-1.383	0.009158	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25484	NAIGRATNTSVPRGSNPQNS			20	SLAY-screened peptide P3834			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGCTATTGGCCGGGCGACTAATACTTCTGTCCCTCGGGGCTCTAATCCCCAGAACAGCTAA	NAIGRATNTSVPRGSNPQNS*	-1.383	8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25485	ILLMYLITSVTRNLD			15	SLAY-screened peptide P3835			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCTCTTGATGTACCTGATTACTAGTGTTACGAGGAACCTCGATTAGGCCTGCACTCTTTAA	ILLMYLITSVTRNLD*ACTL*	-1.382	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25486	CYHLWSEIPLIYSSLNVIDL			20	SLAY-screened peptide P3836			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTACCACCTTTGGTCCGAGATTCCCCTTATCTATAGTTCTTTGAACGTTATCGACCTCTAA	CYHLWSEIPLIYSSLNVIDL*	-1.382	0.000383	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25487	RCSPITSLARSPIKPGLSFI			20	SLAY-screened peptide P3837			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTGCAGCCCGATCACCTCTCTTGCTCGGTCCCCCATTAAGCCTGGGTTGTCGTTCATTTAA	RCSPITSLARSPIKPGLSFI*	-1.382	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25488	TCFDYVPLFLDAMRLPNLLL			20	SLAY-screened peptide P3838			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTGCTTCGACTATGTTCCCCTTTTCCTTGATGCCATGCGCCTCCCTAACCTGCTCCTGTAA	TCFDYVPLFLDAMRLPNLLL*	-1.381	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25489	PHYNCMHIDVV			11	SLAY-screened peptide P3839			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCATTACAACTGCATGCATATTGACGTCGTGTAGTCCAAGAAGGTCTCTCTCGTTGACTAA	PHYNCMHIDVV*SKKVSLVD*	-1.381	2.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25490	EFTENKSLKPTGRHYIQQLYN			21	SLAY-screened peptide P3840			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGTTCACCGAGAACAAGAGCCTCAAGCCGACTGGTCGTCATTATATTCAGCAGCTTTATAAC	EFTENKSLKPTGRHYIQQLYN	-1.381	0.002323	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25491	KHYYPFRASILSMLRRLCSK			20	SLAY-screened peptide P3841			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCATTACTACCCCTTTCGGGCCTCGATTCTCAGTATGCTCCGCCGGCTTTGCTCTAAGTAA	KHYYPFRASILSMLRRLCSK*	-1.381	0.000108	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25492	CAIMTDKVGHGSTITASSHA			20	SLAY-screened peptide P3842			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCCATTATGACGGACAAGGTTGGTCACGGTAGTACTATCACGGCGAGTTCCCATGCCTAA	CAIMTDKVGHGSTITASSHA*	-1.381	0.000699	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25493	RPCARPATDFRDRLVSTDAP			20	SLAY-screened peptide P3843			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCCTGCGCCCGTCCTGCGACGGATTTCCGTGATCGCCTTGTTAGTACCGACGCCCCCTAA	RPCARPATDFRDRLVSTDAP*	-1.38	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25494	TGVDPCFKHTRYCYSILEIA			20	SLAY-screened peptide P3844			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGGTGTCGATCCTTGTTTTAAGCATACTAGGTACTGCTATTCTATTCTTGAGATTGCGTAA	TGVDPCFKHTRYCYSILEIA*	-1.38	0.043425	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25495	LFHHSYSSTHDVLIAHHVPR			20	SLAY-screened peptide P3845			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTTCCATCACTCGTACAGTAGCACGCACGATGTTTTGATCGCGCACCATGTTCCCAGGTAA	LFHHSYSSTHDVLIAHHVPR*	-1.38	0.000231	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25496	GLNSRYSTCQ			10	SLAY-screened peptide P3846			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCTCAATAGTCGCTACTCTACGTGTCAGTAGCACTGGCCTCTTATGCATGAGTGTACGTAA	GLNSRYSTCQ*HWPLMHECT*	-1.379	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25497	VDWRCLNHFFRYEDSPFTVT			20	SLAY-screened peptide P3847			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGGACTGGAGGTGCCTTAATCATTTTTTCCGGTACGAGGATTCTCCTTTCACTGTTACTTAA	VDWRCLNHFFRYEDSPFTVT*	-1.379	0.02407	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25498	ISSDYLLRPGLSWDTSSYCVN			21	SLAY-screened peptide P3848			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAGCTCCGATTATCTCTTGCGCCCCGGCTTGTCCTGGGACACCTCGAGCTACTGTGTTAAC	ISSDYLLRPGLSWDTSSYCVN	-1.379	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25499	CSRIGCPNHLNSRTC			15	SLAY-screened peptide P3849			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTCTAGGATTGGGTGTCCCAACCACCTCAATTCTCGGACTTGTTAGAGTCACTGTAGTTAA	CSRIGCPNHLNSRTC*SHCS*	-1.379	0.000177	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25500	DRPHLYHSCRPSPNPMLMHA			20	SLAY-screened peptide P3850			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCGTCCTCACCTGTACCATTCTTGCCGTCCCTCGCCTAATCCGATGCTTATGCACGCTTAA	DRPHLYHSCRPSPNPMLMHA*	-1.379	0.002388	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25501	RKLRLYHPVGVTR			13	SLAY-screened peptide P3851			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAAGTTGAGGCTTTACCATCCCGTTGGCGTGACCCGCTAGACGGACCTCAAGACTATTTAA	RKLRLYHPVGVTR*TDLKTI*	-1.379	0.016676	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25502	PHRIRRAITATRRILRWARRN			21	SLAY-predicted active peptide P3852			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCACCGCATCCGGCGGGCTATAACTGCGACTCGGCGTATATTACGGTGGGCTCGACGTAAC	PHRIRRAITATRRILRWARRN	-1.379	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25503	GPDSNLSQDNSYYSVCYVLA			20	SLAY-predicted active peptide P3853			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCCTGACTCGAACCTCTCGCAGGACAATAGCTACTATTCTGTTTGCTATGTGCTGGCTTAA	GPDSNLSQDNSYYSVCYVLA*	-1.378	0.000222	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25504	SFWVPANGSPRNHHSGRSPH			20	SLAY-predicted active peptide P3854			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTTTTGGGTGCCCGCTAATGGTAGTCCTCGCAACCATCACTCTGGTCGTTCCCCGCATTAA	SFWVPANGSPRNHHSGRSPH*	-1.378	0.001094	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25505	NQARLASIPSNSYTKRLDEI			20	SLAY-predicted active peptide P3855			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCAGGCTCGTCTGGCCTCTATCCCTTCCAATAGCTACACTAAGAGGCTCGATGAGATTTAA	NQARLASIPSNSYTKRLDEI*	-1.378	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25506	VSCDHNKYNT			10	SLAY-screened peptide P3856			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGAGCTGTGATCATAATAAGTACAATACTTAGACGCAGGCTGAGGCCATGACCCTTTCTTAA	VSCDHNKYNT*TQAEAMTLS*	-1.378	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25507	LAADTCDWYV			10	SLAY-screened peptide P3857			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGCTGCTGACACTTGTGATTGGTATGTGTAGACCATGCAGGTTTCTTTCTCTGCCCCTTAA	LAADTCDWYV*TMQVSFSAP*	-1.378	0.008624	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25508	SFRAVSCLNPHNCEFLCYFI			20	SLAY-screened peptide P3858			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTTTCGCGCCGTTAGTTGTCTCAACCCCCATAACTGCGAGTTTTTGTGCTATTTTATCTAA	SFRAVSCLNPHNCEFLCYFI*	-1.378	0.007492	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25509	LEVLWYLHSIAIYLCSGSKS			20	SLAY-screened peptide P3859			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGAGGTTCTCTGGTATCTCCACTCTATTGCCATCTATCTCTGCTCGGGTTCTAAGAGCTAA	LEVLWYLHSIAIYLCSGSKS*	-1.378	1.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25510	PH			2	SLAY-screened peptide P3860			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCACTAGCGCATCATCGACAGCCTGCTGCCCAGTAACCGCACGTCTAACACGCGCACTTAA	PH*RIIDSLLPSNRTSNTRT*	-1.378	0.001318	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25511	PRFNLINLITHAFRCLSLKY			20	SLAY-screened peptide P3861			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGGTTCAATTTGATCAACCTCATCACGCACGCTTTTCGGTGCCTCTCCCTGAAGTATTAA	PRFNLINLITHAFRCLSLKY*	-1.378	0.014063	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25512	NFGHSTNKLYNDPNCTIVVR			20	SLAY-screened peptide P3862			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTTTGGTCATTCTACCAATAAGTTGTACAACGACCCCAATTGCACTATCGTGGTTAGGTAA	NFGHSTNKLYNDPNCTIVVR*	-1.377	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25513	SVGPHRSSGTFFILISFGPP			20	SLAY-screened peptide P3863			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGTCGGCCCTCACCGCTCCTCGGGCACCTTTTTTATCTTGATCTCGTTTGGCCCTCCTTAA	SVGPHRSSGTFFILISFGPP*	-1.377	0.046086	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25514	RTPYPQNIWGPFTSEDPTLV			20	SLAY-screened peptide P3864			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGACGCCCTACCCCCAGAACATCTGGGGCCCCTTCACCAGCGAGGACCCTACCTTGGTTTAA	RTPYPQNIWGPFTSEDPTLV*	-1.377	0.024348	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25515	VIPPTRHHGCNPTPPWLMLT			20	SLAY-screened peptide P3865			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTATCCCGCCGACCAGGCACCACGGCTGCAACCCTACGCCCCCCTGGCTCATGCTGACTTAA	VIPPTRHHGCNPTPPWLMLT*	-1.376	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25516	GPDNIRAFGSDYSNPISSNT			20	SLAY-screened peptide P3866			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCCTGACAATATCCGCGCTTTTGGCAGTGATTACAGCAATCCTATTTCCAGCAACACCTAA	GPDNIRAFGSDYSNPISSNT*	-1.376	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25517	CHPPAPRHARSALLALPL			18	SLAY-screened peptide P3867			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCATCCCCCAGCTCCACGCCACGCCCGTTCCGCTTTATTGGCTCTACCACTCTGAGCTCTA	CHPPAPRHARSALLALPL*AL	-1.376	0.001816	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25518	HPPTALILTLIGAITRSSN			19	SLAY-screened peptide P3868			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCGCCCACTGCACTTATACTTACTTTAATTGGGGCTATAACTAGATCCTCTAACTGAGTA	HPPTALILTLIGAITRSSN*V	-1.376	0.002117	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25519	RAMSPYYLNPLLSPCVHFLT			20	SLAY-screened peptide P3869			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGCTATGTCTCCCTACTACCTGAACCCTCTGCTGAGCCCCTGCGTGCATTTCCTCACCTAA	RAMSPYYLNPLLSPCVHFLT*	-1.376	0.012069	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25520	PLQPFYVYLNRML			13	SLAY-screened peptide P3870			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTCAGCCCTTCTACGTTTATCTTAATCGTATGCTGTAGCCGCGGTATACGAGGAACTAA	PLQPFYVYLNRML*PRYTRN*	-1.376	8.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25521	TASRLTKITTRRSSSSSALLN			21	SLAY-screened peptide P3871			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGCCTCCCGCCTCACTAAGATTACTACTAGACGATCTTCTTCTTCATCGGCATTACTTAAC	TASRLTKITTRRSSSSSALLN	-1.376	0.000848	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25522	HLRNCFDGLLITRYAHSVLG			20	SLAY-screened peptide P3872			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCTTCGCAACTGCTTTGATGGCCTGCTCATTACTCGGTACGCTCATAGCGTGTTGGGTTAA	HLRNCFDGLLITRYAHSVLG*	-1.376	0.000736	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25523	SIYVFISLYHVWRLRTLNRTN			21	SLAY-screened peptide P3873			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTATTTACGTCTTTATTAGCCTGTACCACGTTTGGCGGTTGCGCACTCTTAATAGGACTAAC	SIYVFISLYHVWRLRTLNRTN	-1.376	0.003082	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25524	HPPHHSWDFNPFRGCYDALS			20	SLAY-screened peptide P3874			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCGCCCCATCACAGCTGGGACTTTAACCCTTTTCGGGGCTGCTACGATGCGCTTAGCTAA	HPPHHSWDFNPFRGCYDALS*	-1.375	0.000749	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25525	QTWTEGLGHNRGGLIQTHPR			20	SLAY-screened peptide P3875			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGACCTGGACCGAGGGGCTCGGCCACAACAGGGGTGGGCTTATTCAGACTCATCCTCGCTAA	QTWTEGLGHNRGGLIQTHPR*	-1.375	2.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25526	GHVLSHSNGVDMLERTTTEE			20	SLAY-screened peptide P3876			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCATGTTCTCAGTCATTCCAATGGTGTCGACATGCTGGAGAGGACTACTACTGAGGAGTAA	GHVLSHSNGVDMLERTTTEE*	-1.375	0.003897	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25527	CS			2	SLAY-screened peptide P3877			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTCCTAGGCCTGGCTCAGTGAGCATCACCCCGGCTAGTAGCTTTCTAATATTTCCTGTTAA	CS*AWLSEHHPG**LSNISC*	-1.375	0.015532	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25528	HRPAFRCWTY			10	SLAY-screened peptide P3878			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCGGCCGGCTTTTCGTTGTTGGACCTACTAGTAGCGCGGGCCCCCGTAGCCTACCGTCTAA	HRPAFRCWTY**RGPP*PTV*	-1.375	0.021618	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25529	ASRTHLHATTPCLDTGIAHR			20	SLAY-screened peptide P3879			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTCGCGGACCCACCTCCATGCCACGACGCCCTGCCTGGATACTGGTATCGCTCACCGGTAA	ASRTHLHATTPCLDTGIAHR*	-1.375	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25530	HTRCCGDPSSPKHLARPMRD			20	SLAY-screened peptide P3880			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACTCGCTGCTGCGGCGACCCCTCGTCCCCTAAGCACTTGGCTAGGCCCATGAGGGATTAA	HTRCCGDPSSPKHLARPMRD*	-1.374	0.006122	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25531	LTIHESSCKSVRFFPYNDIY			20	SLAY-screened peptide P3881			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGACCATTCATGAGAGCTCGTGTAAGTCGGTTCGTTTTTTTCCGTATAATGATATTTATTAA	LTIHESSCKSVRFFPYNDIY*	-1.374	0.00434	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25532	CLYYPLFLSTTLSPSLTF			18	SLAY-screened peptide P3882			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTTTATTATCCCTTGTTCTTGAGTACTACTTTGTCGCCCTCTCTTACTTTTTAGACCTAA	CLYYPLFLSTTLSPSLTF*T*	-1.374	0.008765	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25533	TTFALPFMARSLSSHKRLIA			20	SLAY-screened peptide P3883			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTACTTTTGCCCTCCCCTTTATGGCGCGCTCTCTCAGCAGTCATAAGCGGCTTATTGCCTAA	TTFALPFMARSLSSHKRLIA*	-1.374	0.001539	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25534	LPIREYDFWPVTPLYAYSPP			20	SLAY-screened peptide P3884			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCTATTCGCGAGTATGACTTTTGGCCCGTGACTCCCCTTTATGCGTATAGTCCGCCTTAA	LPIREYDFWPVTPLYAYSPP*	-1.374	8.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25535	LGYYGRNVTALELPRILPQAA			21	SLAY-screened peptide P3885			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGGTTACTACGGTCGCAACGTCACTGCCCTCGAGCTCCCGCGTATCCTCCCGCAGGCCGCC	LGYYGRNVTALELPRILPQAA	-1.374	0.012225	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25536	PH			2	SLAY-screened peptide P3886			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCATTAGACCTATGATATGAATATTCTCCCGCACGCGGCCGGTTGCGTCCTCATGGCCTAA	PH*TYDMNILPHAAGCVLMA*	-1.373	0.008456	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25537	TRSHYKSCALDSPFYHHMHW			20	SLAY-screened peptide P3887			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCGCTCGCATTATAAGTCGTGCGCGCTGGACTCCCCGTTTTATCACCATATGCACTGGTAA	TRSHYKSCALDSPFYHHMHW*	-1.373	0.000184	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25538	DPDPCII			7	SLAY-screened peptide P3888			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCCCGACCCCTGTATTATTTAGAATCGGCGTCTTTACCATTAGGGTAACGTTTTCCGGTAA	DPDPCII*NRRLYH*GNVFR*	-1.373	0.005154	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25539	LTHLSVSPNRISARCTCLTC			20	SLAY-screened peptide P3889			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCACCCATCTCTCGGTGTCCCCCAATCGCATTTCCGCGCGGTGCACGTGTCTTACTTGCTAA	LTHLSVSPNRISARCTCLTC*	-1.373	0.01119	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25540	TIMAHLRQPISRGPPEYFVG			20	SLAY-screened peptide P3890			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTATCATGGCCCACCTCCGCCAGCCTATCAGTCGTGGTCCCCCTGAGTATTTTGTTGGCTAA	TIMAHLRQPISRGPPEYFVG*	-1.373	0.048438	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25541	AGHHSGPLTAIRSNITTCKR			20	SLAY-screened peptide P3891			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGGCCATCATTCCGGTCCCCTTACGGCGATTCGCTCGAACATTACTACCTGTAAGCGTTAA	AGHHSGPLTAIRSNITTCKR*	-1.372	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25542	LVDNYRSSIDTEFCNPRDPS			20	SLAY-screened peptide P3892			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGTTGACAACTACCGTAGCAGTATCGACACTGAGTTTTGTAACCCTCGCGATCCCAGTTAA	LVDNYRSSIDTEFCNPRDPS*	-1.372	0.000849	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25543	RVAGPDADSINKLDNHDLNN			20	SLAY-screened peptide P3893			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGTTGCGGGCCCTGACGCCGATAGCATTAACAAGCTTGATAATCATGACCTTAACAATTAA	RVAGPDADSINKLDNHDLNN*	-1.372	0.042403	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25544	LINLSAPSHSDSSEHIPNNQ			20	SLAY-screened peptide P3894			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATTAATCTCTCTGCCCCCTCCCACTCTGACAGTTCTGAGCATATTCCTAACAATCAGTAA	LINLSAPSHSDSSEHIPNNQ*	-1.372	0.000327	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25545	LHYGSFCDLYHFFKYINGSV			20	SLAY-screened peptide P3895			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCATTACGGCTCTTTTTGTGATCTCTATCACTTTTTTAAGTATATCAATGGCTCCGTTTAA	LHYGSFCDLYHFFKYINGSV*	-1.372	0.000415	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25546	DLQNVPTNLKVYYRVHQLRC			20	SLAY-screened peptide P3896			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCTCCAGAACGTTCCTACTAACCTCAAGGTTTATTATAGGGTGCACCAGCTTAGGTGCTAA	DLQNVPTNLKVYYRVHQLRC*	-1.371	0.007733	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25547	NSVYDMFPYITVRRSW			16	SLAY-screened peptide P3897			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTCCGTCTACGATATGTTTCCGTACATTACGGTTCGCAGGTCCTGGTAGGTCATTTACTAA	NSVYDMFPYITVRRSW*VIY*	-1.371	0.005656	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25548	MKLFSRGRLPANYATFFSRN			20	SLAY-screened peptide P3898			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGAAGCTTTTCTCGAGGGGCCGCTTGCCCGCGAACTACGCTACGTTCTTCTCTCGTAATTAA	MKLFSRGRLPANYATFFSRN*	-1.371	0.036574	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25549	SNTSNPFYSALFDLNPRLYS			20	SLAY-screened peptide P3899			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGAATACTTCTAATCCTTTCTATAGTGCGCTTTTTGATCTTAACCCGCGGCTGTATTCTTAA	SNTSNPFYSALFDLNPRLYS*	-1.371	7.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25550	LVLVYRGFLLVFVLMAGTTPN			21	SLAY-screened peptide P3900			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGTGCTTGTGTATCGTGGTTTTTTACTCGTTTTCGTTCTCATGGCTGGTACTACACCTAAC	LVLVYRGFLLVFVLMAGTTPN	-1.371	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25551	LTPPYTSDEEFLTNTHSHQN			20	SLAY-screened peptide P3901			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGACCCCGCCGTATACGTCTGACGAGGAGTTTCTGACGAACACTCATAGTCATCAGAATTAA	LTPPYTSDEEFLTNTHSHQN*	-1.371	0.005418	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25552	FCVPSIHFSGSILRPNRVFY			20	SLAY-screened peptide P3902			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTGTGTTCCGAGTATTCATTTCTCCGGTAGTATCCTTCGTCCGAACCGCGTTTTCTATTAA	FCVPSIHFSGSILRPNRVFY*	-1.371	0.022385	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25553	PLTLSVTLLNKFMSGFALSW			20	SLAY-screened peptide P3903			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTGACTCTTAGTGTCACCCTGCTTAATAAGTTCATGTCTGGGTTCGCGCTGAGTTGGTAA	PLTLSVTLLNKFMSGFALSW*	-1.371	0.004689	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25554	TNPDAYDLASGNQFQSQFMI			20	SLAY-screened peptide P3904			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAACCCCGATGCTTACGACCTGGCTTCTGGCAACCAGTTTCAGAGCCAGTTCATGATCTAA	TNPDAYDLASGNQFQSQFMI*	-1.37	0.003656	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25555	YGSTFRTFASFRLHNVQHYT			20	SLAY-screened peptide P3905			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGGTTCCACTTTTAGGACTTTTGCTTCCTTTAGGCTGCATAATGTTCAGCACTACACCTAA	YGSTFRTFASFRLHNVQHYT*	-1.37	0.005105	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25556	CLM			3	SLAY-screened peptide P3906			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTCATGTAGGCGCTTGGCGCCGATGCCACCAGTAATGCGCATTACCGTACGAAGTTGTAA	CLM*ALGADATSNAHYRTKL*	-1.37	0.003416	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25557	CRNRLFPFYEL			11	SLAY-screened peptide P3907			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCGCAACCGTCTCTTCCCTTTTTATGAGCTTTAGCCTAACAATAATTCCCGCTGTTCCTAA	CRNRLFPFYEL*PNNNSRCS*	-1.37	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25558	RMSSRILNCTPWRATKMMAI			20	SLAY-screened peptide P3908			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATGTCGTCTCGTATCTTGAATTGTACGCCCTGGCGTGCCACTAAGATGATGGCGATCTAA	RMSSRILNCTPWRATKMMAI*	-1.37	0.00079	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25559	SPLRFISFRHYIARGPPFIH			20	SLAY-screened peptide P3909			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCCCCTCAGGTTCATTTCTTTTCGTCACTATATTGCTCGCGGGCCTCCTTTTATCCATTAA	SPLRFISFRHYIARGPPFIH*	-1.369	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25560	HYAAVLLFCRPLPDLRRSNG			20	SLAY-screened peptide P3910			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTATGCCGCTGTGCTGCTCTTTTGTCGCCCCCTTCCTGATCTTAGGCGGTCTAATGGTTAA	HYAAVLLFCRPLPDLRRSNG*	-1.369	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25561	HSSFNLLPPCHYVALPLLYA			20	SLAY-screened peptide P3911			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAGTTCCTTTAACCTGCTCCCTCCGTGCCATTACGTGGCGCTTCCTCTGCTTTATGCGTAA	HSSFNLLPPCHYVALPLLYA*	-1.369	0.003404	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25562	YPVIILTPAPLPLPTSRRVFN			21	SLAY-screened peptide P3912			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCCTGTGATCATTCTCACTCCTGCGCCACTCCCATTACCGACCTCCAGGAGAGTGTTTAAC	YPVIILTPAPLPLPTSRRVFN	-1.369	0.00069	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25563	HDMSSHCYLIDHRYSTDHTA			20	SLAY-screened peptide P3913			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGATATGAGTAGCCATTGCTACTTGATTGATCACCGTTACTCTACCGATCATACTGCGTAA	HDMSSHCYLIDHRYSTDHTA*	-1.368	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25564	ADVPEPCVTMSNGNPVARMR			20	SLAY-screened peptide P3914			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGACGTCCCTGAGCCCTGCGTCACGATGTCTAATGGCAATCCCGTTGCGCGTATGCGGTAA	ADVPEPCVTMSNGNPVARMR*	-1.368	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25565	TTDVTSHPFVDKSVHLHVET			20	SLAY-screened peptide P3915			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACCGATGTGACTTCCCATCCGTTTGTCGATAAGTCTGTGCACCTCCACGTGGAGACGTAA	TTDVTSHPFVDKSVHLHVET*	-1.368	0.024208	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25566	PCWFSGSPLDPVYTHLASAS			20	SLAY-screened peptide P3916			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGTTGGTTCTCGGGTTCGCCGCTCGATCCGGTTTACACCCATCTTGCTTCTGCCTCGTAA	PCWFSGSPLDPVYTHLASAS*	-1.368	0.001948	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25567	NHPHTLVPK			9	SLAY-screened peptide P3917			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCACCCGCACACGTTGGTCCCCAAGTAGAACGTCCCCTGCGGTTGCGCTACCTACTCCTAA	NHPHTLVPK*NVPCGCATYS*	-1.368	2.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25568	NHFMCSGFSYFPWYLIR			17	SLAY-screened peptide P3918			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCACTTTATGTGTTCGGGCTTTTCGTATTTCCCCTGGTACCTCATCCGCTAGCCCATGTAA	NHFMCSGFSYFPWYLIR*PM*	-1.367	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25569	THIAFSKAYMPATIERPRIC			20	SLAY-screened peptide P3919			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCACATTGCCTTCTCCAAGGCCTACATGCCTGCCACCATTGAGCGTCCTCGGATTTGCTAA	THIAFSKAYMPATIERPRIC*	-1.366	0.047232	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25570	SMWGNKLLTSRRHMQTQLSH			20	SLAY-screened peptide P3920			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGATGTGGGGTAATAAGCTCTTGACGTCGAGGAGGCATATGCAGACCCAGCTCTCTCATTAA	SMWGNKLLTSRRHMQTQLSH*	-1.366	0.001596	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25571	DLYT			4	SLAY-screened peptide P3921			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCTCTACACCTAGTACCGCCTTACGCAGTGCGGTATTCATCTCTCCCTGCCTAATTACTAA	DLYT*YRLTQCGIHLSLPNY*	-1.365	0.002583	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25572	LSNVFSSLVSN			11	SLAY-screened peptide P3922			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCCAATGTTTTCAGCTCCCTTGTGAGTAACTAGAGTTGGCCGCACAACAATAGCCACTAA	LSNVFSSLVSN*SWPHNNSH*	-1.365	0.006514	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25573	PMRSCTFDYNRHYMRSAYSR			20	SLAY-screened peptide P3923			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATGCGCAGTTGCACCTTTGACTATAATCGCCATTACATGCGTAGCGCTTATTCTAGGTAA	PMRSCTFDYNRHYMRSAYSR*	-1.365	0.012102	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25574	WTGRLVPTTTSGIDATNHRV			20	SLAY-screened peptide P3924			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGACCGGGAGGCTGGTGCCCACTACGACCTCTGGTATTGATGCTACTAACCATCGCGTTTAA	WTGRLVPTTTSGIDATNHRV*	-1.364	0.022484	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25575	DVCLDCNNIFIHFLIDHCV			19	SLAY-screened peptide P3925			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATGTTTGTCTGGACTGCAACAATATTTTTATCCATTTTTTGATTGACCACTGTGTTTAGTAA	DVCLDCNNIFIHFLIDHCV**	-1.364	0.001553	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25576	TRVPHSP			7	SLAY-screened peptide P3926			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCGCGTTCCCCATTCCCCTTAGTCGCTTAACGGCCCTAGGCTTCTGTTCTCCCCGTCCTAA	TRVPHSP*SLNGPRLLFSPS*	-1.364	0.002301	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25577	AMNDRPLL			8	SLAY-screened peptide P3927			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTATGAACGATCGCCCCCTGCTCTAGGCTAGTTTTAGCGGTATTTTTTATCGCATTACTTAA	AMNDRPLL*ASFSGIFYRIT*	-1.364	5.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25578	STSTRCNPFRTFNAVDYILF			20	SLAY-screened peptide P3928			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTACGAGTACTCGTTGTAACCCTTTTCGGACCTTCAATGCCGTTGACTATATTCTCTTCTAA	STSTRCNPFRTFNAVDYILF*	-1.364	0.003385	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25579	DVISLPETRLMWWSAYPTCL			20	SLAY-screened peptide P3929			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATGTCATCTCGCTTCCTGAGACGCGTCTGATGTGGTGGTCTGCCTATCCTACTTGCCTGTAA	DVISLPETRLMWWSAYPTCL*	-1.364	0.000462	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25580	SADGSAGYPSGFCVSFLQVL			20	SLAY-screened peptide P3930			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGCGGATGGCAGTGCTGGTTACCCCTCGGGCTTTTGTGTTTCTTTTCTGCAGGTCCTGTAA	SADGSAGYPSGFCVSFLQVL*	-1.364	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25581	RCIFLTNNAFIPYRHKHCIL			20	SLAY-screened peptide P3931			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTGTATCTTTCTTACCAACAATGCTTTTATCCCTTATCGTCACAAGCACTGTATTCTGTAA	RCIFLTNNAFIPYRHKHCIL*	-1.364	0.010531	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25582	ASTMSTTS			8	SLAY-screened peptide P3932			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTCTACTATGTCTACTACTAGTTAGCTCCTGATCTTCTTAACCCCATTCGGAGCATCTAAC	ASTMSTTS*LLIFLTPFGASN	-1.363	0.007957	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25583	IRITTGYCPAERRNMPIAAS			20	SLAY-screened peptide P3933			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCGTATTACCACTGGCTACTGCCCTGCGGAGAGGCGCAATATGCCGATTGCTGCTAGTTAA	IRITTGYCPAERRNMPIAAS*	-1.363	0.001755	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25584	FNLHIYSWCSHVTI			14	SLAY-screened peptide P3934			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTAACCTTCATATTTATTCTTGGTGCTCCCACGTCACTATCTGATCAATAACGCTTAGTAAC	FNLHIYSWCSHVTI*SITLSN	-1.362	0.001452	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25585	AFRAIVDAPITWAPVTSLVC			20	SLAY-screened peptide P3935			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTTCCGCGCGATCGTGGACGCGCCTATCACTTGGGCCCCTGTTACTTCCTTGGTGTGTTAA	AFRAIVDAPITWAPVTSLVC*	-1.362	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25586	TNTVGTFVHFPHSIYWYRPT			20	SLAY-screened peptide P3936			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAATACCGTGGGGACCTTCGTCCACTTCCCTCATTCCATTTATTGGTACAGGCCCACCTAA	TNTVGTFVHFPHSIYWYRPT*	-1.362	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25587	PCFPRTHTRASTVYLTSRSK			20	SLAY-screened peptide P3937			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGTTTTCCTCGCACTCACACTCGGGCTAGCACGGTTTACCTCACGTCGCGGTCTAAGTAA	PCFPRTHTRASTVYLTSRSK*	-1.362	0.017899	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25588	HRYTPVVVGWPIPSSRTLMP			20	SLAY-screened peptide P3938			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCGGTACACTCCTGTTGTTGTTGGCTGGCCGATCCCGTCGTCTCGCACTTTGATGCCTTAA	HRYTPVVVGWPIPSSRTLMP*	-1.362	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25589	CTRFGSYDSISTSSVHFQFV			20	SLAY-screened peptide P3939			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCACTAGGTTTGGCTCTTATGACAGTATTTCGACGAGTAGTGTCCATTTTCAGTTTGTGTAA	CTRFGSYDSISTSSVHFQFV*	-1.362	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25590	PPGPVDCPPRIPHNRQKFPT			20	SLAY-screened peptide P3940			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCCGGTCCCGTTGATTGTCCTCCCCGCATCCCCCATAACCGGCAGAAGTTTCCTACTTAA	PPGPVDCPPRIPHNRQKFPT*	-1.361	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25591	ADPENSSLRCAPIFPGFTRT			20	SLAY-screened peptide P3941			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGACCCTGAGAACTCCTCGCTTCGTTGCGCGCCCATCTTCCCCGGTTTTACCAGGACCTAA	ADPENSSLRCAPIFPGFTRT*	-1.361	0.003041	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25592	PEWRKSWAAYSRAFLV			16	SLAY-screened peptide P3942			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGAGTGGAGGAAGTCTTGGGCTGCTTACTCCCGCGCTTTTTTAGTATGAGTAACTGAGTAA	PEWRKSWAAYSRAFLV*VTE*	-1.361	0.000463	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25593	RPNTRPSL			8	SLAY-screened peptide P3943			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCTAACACCCGGCCTAGCCTTTAGCATCTTAATCGCGAGTAAGTCGACCTGCAGGCATGC	RPNTRPSL*HLNRE*VDLQAC	-1.361	0.010181	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25594	SPHMCRRRVNNRWFVGALAF			20	SLAY-screened peptide P3944			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCCGCATATGTGTCGCCGTCGGGTTAATAACCGCTGGTTTGTTGGCGCGCTTGCCTTCTAA	SPHMCRRRVNNRWFVGALAF*	-1.361	0.000445	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25595	HIKEPYSLNCRDLFYLARRI			20	SLAY-screened peptide P3945			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATATCAAGGAGCCTTATAGCCTTAATTGTCGCGACCTTTTCTACTTGGCCCGTCGTATCTAA	HIKEPYSLNCRDLFYLARRI*	-1.361	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25596	RDTHRWHVLANNSFRGGPRV			20	SLAY-screened peptide P3946			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGATACTCACCGGTGGCATGTTCTGGCTAACAACTCGTTTAGGGGGGGTCCGAGGGTGTAA	RDTHRWHVLANNSFRGGPRV*	-1.361	0.007492	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25597	FRRSPL			6	SLAY-screened peptide P3947			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCGTCGCTCCCCTCTGTAGCTCATTGCCCTGTCTGCCTCCCCTTCGACGGCTCCCAGCTAA	FRRSPL*LIALSASPSTAPS*	-1.361	0.020672	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25598	HPPSIVLRALYKYVLSPFLS			20	SLAY-screened peptide P3948			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCTCCGAGTATTGTGTTGAGGGCCCTGTATAAGTACGTCTTGTCCCCGTTTCTTTCCTAA	HPPSIVLRALYKYVLSPFLS*	-1.361	0.031671	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25599	CTLQFHDLIYTILPLPLYNI			20	SLAY-screened peptide P3949			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCACCCTGCAGTTTCACGATCTGATCTATACTATCCTGCCCCTTCCTCTGTATAACATTTAA	CTLQFHDLIYTILPLPLYNI*	-1.36	0.027099	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25600	GCDSYSTILLNCINPCYNYL			20	SLAY-screened peptide P3950			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTGCGATAGTTACTCGACGATTCTTTTGAACTGTATTAACCCGTGCTATAATTATCTCTAA	GCDSYSTILLNCINPCYNYL*	-1.36	0.007801	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25601	AFYAGSLLRPEPEAPDQATP			20	SLAY-screened peptide P3951			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTTCTACGCTGGCAGTCTGCTCAGGCCGGAGCCGGAGGCGCCTGATCAGGCGACTCCGTAA	AFYAGSLLRPEPEAPDQATP*	-1.36	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25602	ATPSCFCATHHRPWYWW			17	SLAY-screened peptide P3952			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACTCCTAGTTGTTTTTGTGCCACTCACCACCGCCCGTGGTATTGGTGGTAGGCTTGCTAA	ATPSCFCATHHRPWYWW*AC*	-1.36	0.001001	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25603	HAIHDALSVPGNVVVVNWLH			20	SLAY-screened peptide P3953			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCCATCCACGATGCCCTGAGTGTCCCGGGCAACGTCGTCGTTGTTAACTGGCTGCATTAA	HAIHDALSVPGNVVVVNWLH*	-1.36	2.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25604	GSGDHNINIYLCHDSNENNP			20	SLAY-screened peptide P3954			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTCCGGTGATCACAACATCAACATTTACCTTTGTCATGACAGTAATGAGAACAATCCTTAA	GSGDHNINIYLCHDSNENNP*	-1.36	0.010687	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25605	LSSITTGFN			9	SLAY-screened peptide P3955			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCTTCCATTACTACGGGTTTTAATTAGGACAGCTATCTTGCTACGTTGCCTGGCGGCTAA	LSSITTGFN*DSYLATLPGG*	-1.36	0.02565	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25606	RV			2	SLAY-screened peptide P3956			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGTCTAGCCGCATTTTGACTCCTTGAAAGTCGAATTAGATGTGGGCCCCTTACAGTGATTA	RV*PHFDSLKVELDVGPLQ*L	-1.36	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25607	SRNSDVAILLWAQTSSKATY			20	SLAY-screened peptide P3957			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCGGAATTCTGATGTTGCGATTCTCTTGTGGGCCCAGACGAGCAGCAAGGCTACGTATTAA	SRNSDVAILLWAQTSSKATY*	-1.359	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25608	PVCSDRFQYRHWPYTLILYS			20	SLAY-screened peptide P3958			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGTTTGTTCTGATCGTTTTCAGTATCGTCATTGGCCTTATACGCTTATCTTGTACTCGTAA	PVCSDRFQYRHWPYTLILYS*	-1.359	0.007135	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25609	SKSPPHSRYYCCIFLGIPKI			20	SLAY-screened peptide P3959			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTAAGTCGCCGCCGCACAGCCGTTACTACTGCTGTATCTTCCTGGGTATTCCCAAGATCTAA	SKSPPHSRYYCCIFLGIPKI*	-1.359	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25610	IIEHAICTSYPRWFTSITLIN			21	SLAY-screened peptide P3960			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCATCGAGCACGCCATTTGTACTTCTTATCCAAGATGGTTCACATCAATCACATTGATTAAC	IIEHAICTSYPRWFTSITLIN	-1.359	0.000205	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25611	RLQVIY			6	SLAY-screened peptide P3961			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTCCAGGTTATCTATTAGAGCTATGCGCCCCGTCATGAGCTGTGGTAGCGGAAGAGCTAA	RLQVIY*SYAPRHELW*RKS*	-1.359	0.000844	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25612	CAHNKPSFMLA			11	SLAY-screened peptide P3962			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGCTCACAACAAGCCGTCCTTTATGCTGGCCTAGGGCTGTCCGCGGACGCTTTTTCCCTAA	CAHNKPSFMLA*GCPRTLFP*	-1.359	0.047086	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25613	SHLAGFICPLTLFFFSRTPS			20	SLAY-screened peptide P3963			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCATTTGGCTGGTTTCATTTGCCCTCTTACCTTGTTTTTCTTCAGTAGGACTCCCAGTTAA	SHLAGFICPLTLFFFSRTPS*	-1.359	0.003921	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25614	LFNIWLTHFTG			11	SLAY-screened peptide P3964			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTTCAATATTTGGCTGACGCATTTCACCGGTTAGCACGCGCACGATAACGCGATCCGTTAA	LFNIWLTHFTG*HAHDNAIR*	-1.358	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25615	THTLEFRETIDNKYSAYNNY			20	SLAY-screened peptide P3965			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCACACCCTGGAGTTTCGTGAGACGATCGATAATAAGTACTCCGCCTATAACAATTACTAA	THTLEFRETIDNKYSAYNNY*	-1.358	6.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25616	HVLYPVFIHYYPDIITD			17	SLAY-screened peptide P3966			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGTTTTGTACCCCGTCTTCATCCACTATTACCCCGATATTATTACGGATTAGACGAGCTAA	HVLYPVFIHYYPDIITD*TS*	-1.358	0.035055	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25617	RSWLPLCPILTAIRGSTRTVN			21	SLAY-screened peptide P3967			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAGCTGGCTGCCACTTTGCCCGATACTTACCGCCATTCGAGGCAGTACACGAACTGTTAAC	RSWLPLCPILTAIRGSTRTVN	-1.357	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25618	IKRIIDATTNGYVYHTLRPP			20	SLAY-screened peptide P3968			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAAGAGGATCATTGATGCTACGACCAACGGGTATGTGTACCATACCTTGCGTCCCCCCTAA	IKRIIDATTNGYVYHTLRPP*	-1.357	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25619	PFSAW			5	SLAY-screened peptide P3969			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTTCAGCGCCTGGTAGTGCCGTAATATTATTAAGCCTTAGTCCCTCTCCATGTTGCATTAA	PFSAW*CRNIIKP*SLSMLH*	-1.357	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25620	MNCYTGS			7	SLAY-screened peptide P3970			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGAATTGCTATACCGGCTCTTAGCGGTCTTTGTATATGACGCACAACTCCATCTCCTATTAA	MNCYTGS*RSLYMTHNSISY*	-1.357	0.036302	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25621	RKVYNRNLNQWLVFTQIESF			20	SLAY-screened peptide P3971			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAAGGTTTACAATCGTAACCTTAATCAGTGGCTTGTTTTCACTCAGATTGAGTCTTTCTAA	RKVYNRNLNQWLVFTQIESF*	-1.357	3.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25622	SSVSAYRRHDSIRVDLVPFL			20	SLAY-screened peptide P3972			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTCTGTTAGTGCTTATCGCCGTCATGACAGCATCCGCGTTGATCTGGTTCCTTTCCTGTAA	SSVSAYRRHDSIRVDLVPFL*	-1.357	0.004866	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25623	FLSNFERFD			9	SLAY-screened peptide P3973			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCTCTCTAACTTTGAGCGTTTTGATTAGACTCTCATCCCTACGCATAACTTTTTCGATTAA	FLSNFERFD*TLIPTHNFFD*	-1.357	0.011476	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25624	TIRITLFMPSRKYDKFCIDR			20	SLAY-screened peptide P3974			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTATCCGTATTACGCTTTTTATGCCTTCTCGTAAGTACGACAAGTTTTGCATTGACCGTTAA	TIRITLFMPSRKYDKFCIDR*	-1.357	0.009481	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25625	RFLPHYFNINFPIRILNPVY			20	SLAY-screened peptide P3975			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTTTCTTCCGCATTATTTTAATATTAACTTTCCGATCCGCATTCTCAACCCTGTGTACTAA	RFLPHYFNINFPIRILNPVY*	-1.356	0.015544	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25626	LCRPRP			6	SLAY-screened peptide P3976			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTGTCGGCCTAGGCCGTAGGACACCGCTTTTTTTCATTTTCCCTCCATCTTTGTTCACTAA	LCRPRP*DTAFFHFPSIFVH*	-1.356	8.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25627	SSDILYVFLLPPKVRVCCNN			20	SLAY-screened peptide P3977			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTCCGACATTCTCTACGTCTTTTTGTTGCCGCCTAAGGTCCGCGTTTGCTGCAACAATTAA	SSDILYVFLLPPKVRVCCNN*	-1.356	0.01628	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25628	FHCGS			5	SLAY-screened peptide P3978			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCATTGTGGCTCCTAGATCCCGCATAATCTCAAGTAGTATAACATCAACGATGACAATTAA	FHCGS*IPHNLK*YNINDDN*	-1.356	0.018153	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25629	VRSNLSRISVQVPMHFFSYI			20	SLAY-screened peptide P3979			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGAGGAGCAATCTGTCCCGTATTTCCGTGCAGGTGCCTATGCATTTTTTCTCGTATATTTAA	VRSNLSRISVQVPMHFFSYI*	-1.356	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25630	LASPINLYTLCIAIFHPSTP			20	SLAY-screened peptide P3980			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGCTTCGCCCATCAACCTTTATACTCTGTGTATCGCTATCTTCCATCCCTCTACTCCGTAA	LASPINLYTLCIAIFHPSTP*	-1.355	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25631	ATTGTGLCADHRDALDIIRL			20	SLAY-screened peptide P3981			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACTACTGGCACCGGCTTGTGTGCGGACCACCGGGACGCGCTTGATATCATCAGGCTCTAA	ATTGTGLCADHRDALDIIRL*	-1.355	0.029756	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25632	PQRSIAGYI			9	SLAY-screened peptide P3982			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCAGCGTTCCATCGCTGGTTATATCTAGATCATGATCGGGATCTTCAGGCGGATCGCCTAA	PQRSIAGYI*IMIGIFRRIA*	-1.355	0.008331	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25633	PSNCVIRWTLHGLPNTYYRL			20	SLAY-screened peptide P3983			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCCAACTGCGTTATTCGCTGGACTCTGCATGGCCTGCCTAATACCTATTATAGGCTTTAA	PSNCVIRWTLHGLPNTYYRL*	-1.355	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25634	VSSRALTILRSSPNALNYLK			20	SLAY-screened peptide P3984			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTCGTCTCGTGCCTTGACGATCCTTCGCTCGAGCCCGAATGCCCTGAATTACCTTAAGTAA	VSSRALTILRSSPNALNYLK*	-1.355	0.000712	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25635	AAHPCHIDPDYHYLRHILIS			20	SLAY-screened peptide P3985			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCGCACCCGTGTCATATTGACCCGGATTACCATTATCTTCGTCATATCCTGATCTCTTAA	AAHPCHIDPDYHYLRHILIS*	-1.354	0.009018	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25636	RPIRSRLSSTTKSAIRMMTF			20	SLAY-screened peptide P3986			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCGATTCGCAGTCGTCTCAGCAGTACCACGAAGTCTGCTATCCGTATGATGACGTTCTAA	RPIRSRLSSTTKSAIRMMTF*	-1.354	0.010202	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25637	PTPWSYPAWPCLCSYATQFL			20	SLAY-screened peptide P3987			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACCCCTTGGTCCTACCCTGCCTGGCCCTGTCTTTGTTCCTACGCCACCCAGTTCCTGTAA	PTPWSYPAWPCLCSYATQFL*	-1.354	0.000372	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25638	VRHRLPWNKRNNCVIMVNYR			20	SLAY-screened peptide P3988			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCGGCACCGTCTCCCTTGGAACAAGCGCAACAATTGCGTTATTATGGTTAATTATCGTTAA	VRHRLPWNKRNNCVIMVNYR*	-1.354	8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25639	DFFRSN			6	SLAY-screened peptide P3989			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTTCTTTCGGTCGAATTAGGCGACGAAGCGGCTTGCTCTTGCCGCGACCCCTTCCGCGTAA	DFFRSN*ATKRLALAATPSA*	-1.354	0.019799	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25640	SATTSDASWPYEFMYLSNFV			20	SLAY-screened peptide P3990			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGCGACCACCAGCGATGCCTCTTGGCCGTACGAGTTTATGTATCTTAGCAACTTTGTTTAA	SATTSDASWPYEFMYLSNFV*	-1.354	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25641	HPDTADVSSYCMVFPALQIL			20	SLAY-screened peptide P3991			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCTGATACCGCTGATGTTTCGAGTTACTGTATGGTCTTTCCTGCCCTGCAGATCCTCTAA	HPDTADVSSYCMVFPALQIL*	-1.354	0.0045	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25642	AA			2	SLAY-screened peptide P3992			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGCTTAGTCTTTGTGTTAGATCCAGCTTCCTCCGCGCAAGAGCAACGCCGATCCCAATTAA	AA*SLC*IQLPPRKSNADPN*	-1.353	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25643	IDVPSSGVHENTRANALSPV			20	SLAY-screened peptide P3993			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGATGTGCCTTCGAGCGGTGTGCACGAGAATACCAGGGCTAACGCCCTGAGCCCGGTGTAA	IDVPSSGVHENTRANALSPV*	-1.353	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25644	IRISYRILSHQPSLHPSNSR			20	SLAY-screened peptide P3994			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCGGATCAGCTATAGGATCCTTTCGCACCAGCCCAGCCTCCACCCTTCTAATAGTCGGTAA	IRISYRILSHQPSLHPSNSR*	-1.353	0.005525	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25645	SPSCIFSRVFRKLRNLTPSL			20	SLAY-screened peptide P3995			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCCCAGTTGCATCTTCAGCCGTGTTTTTCGGAAGCTCCGTAACCTGACCCCTTCGCTCTAA	SPSCIFSRVFRKLRNLTPSL*	-1.353	0.038953	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25646	LASPWLFRPLPFPHISMSHP			20	SLAY-screened peptide P3996			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGCCTCCCCTTGGCTTTTCAGGCCTCTTCCTTTTCCGCACATCTCCATGTCCCACCCTTAA	LASPWLFRPLPFPHISMSHP*	-1.353	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25647	FAFANSTVNTLPLTTLTSLS			20	SLAY-screened peptide P3997			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGCCTTCGCTAACTCTACCGTCAATACGCTTCCTCTGACTACCCTTACTTCTCTGAGTTAA	FAFANSTVNTLPLTTLTSLS*	-1.353	0.020956	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25648	TLLSHWEARGTSTFVANPSH			20	SLAY-screened peptide P3998			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTTCTGTCTCACTGGGAGGCCCGCGGCACCAGTACTTTTGTGGCCAACCCTTCCCATTAA	TLLSHWEARGTSTFVANPSH*	-1.352	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25649	PVHDAQWRATRYAIVIVFPS			20	SLAY-screened peptide P3999			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGTCCATGATGCTCAGTGGCGCGCGACTAGGTACGCGATTGTCATCGTGTTTCCCTCTTAA	PVHDAQWRATRYAIVIVFPS*	-1.352	0.000177	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25650	HLGGPSSESRSSDPYVANFC			20	SLAY-screened peptide P4000			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCTTGGGGGGCCTAGCAGCGAGAGCCGTTCGAGTGATCCCTACGTTGCGAATTTTTGCTAA	HLGGPSSESRSSDPYVANFC*	-1.352	0.004719	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25651	IIL			3	SLAY-screened peptide P4001			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCATCCTTTAGGTCTCCTCTAACCAGGGTCATATCTTTACGACGTCCAGCTTTGGCACCTAA	IIL*VSSNQGHIFTTSSFGT*	-1.352	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25652	TFNLLIRRDILS			12	SLAY-screened peptide P4002			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTTTAACCTGCTCATTCGTCGCGACATCCTTAGTTGAAGATCTATCCCGAGACTCTCTAAC	TFNLLIRRDILS*RSIPRLSN	-1.352	0.036203	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25653	HLVNLIQWLINHCSQCSPRF			20	SLAY-screened peptide P4003			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCTCGTGAACCTCATTCAGTGGCTGATTAATCATTGTTCCCAGTGCAGTCCGCGCTTTTAA	HLVNLIQWLINHCSQCSPRF*	-1.351	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25654	WLWDRTLSTPLLVNSQCHDY			20	SLAY-screened peptide P4004			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCTGTGGGACCGTACTCTTAGCACTCCGCTCCTGGTTAATTCGCAGTGTCATGACTATTAA	WLWDRTLSTPLLVNSQCHDY*	-1.351	5.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25655	TSTTWVLDITPHPKYPRRGL			20	SLAY-screened peptide P4005			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCCACGACGTGGGTTCTCGATATCACCCCTCATCCCAAGTACCCCCGGCGGGGTCTGTAA	TSTTWVLDITPHPKYPRRGL*	-1.351	0.005306	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25656	ALRLEGLISFPMLTAHYTGW			20	SLAY-screened peptide P4006			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCTCCGCTTGGAGGGTTTGATTAGTTTTCCCATGCTTACCGCGCATTATACGGGGTGGTAA	ALRLEGLISFPMLTAHYTGW*	-1.35	0.007726	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25657	HFVHPRCHNSWYDSLDNFDL			20	SLAY-screened peptide P4007			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTTTGTTCACCCCCGTTGTCACAATAGTTGGTATGACTCCCTGGACAACTTCGATTTGTAA	HFVHPRCHNSWYDSLDNFDL*	-1.35	0.025335	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25658	PACNTRALQATNGNFSIHK			19	SLAY-screened peptide P4008			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCTTGTAATACTCGTGCGCTTCAGGCCACTAATGGCAACTTCTCTATTCATAAGTAGTAA	PACNTRALQATNGNFSIHK**	-1.35	0.02322	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25659	PIQVRAPLDKTLKRT			15	SLAY-screened peptide P4009			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATCCAGGTCAGGGCGCCTCTGGACAAGACCCTGAAGCGCACGTAGTTCTATTACGATTAA	PIQVRAPLDKTLKRT*FYYD*	-1.35	0.033577	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25660	CHLHTTEHHRRNNYQSYNHI			20	SLAY-screened peptide P4010			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCATCTTCACACGACTGAGCACCACCGCCGCAATAACTATCAGTCGTATAACCATATTTAA	CHLHTTEHHRRNNYQSYNHI*	-1.35	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25661	WSNADILLHEHSVTE			15	SLAY-screened peptide P4011			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGAGTAACGCTGACATTCTCCTCCACGAACATTCTGTAACTGAGTAAGTCGACCTGCAGGCA	WSNADILLHEHSVTE*VDLQA	-1.35	0.01993	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25662	AAK			3	SLAY-screened peptide P4012			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCCAAGTAGGCCCATGATCCGATGATTCGGTGTCCGGCTTATGATCATATGGGCATCTAA	AAK*AHDPMIRCPAYDHMGI*	-1.35	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25663	CSPTHPHPSRTTPIASPSQR			20	SLAY-screened peptide P4013			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTCGCCTACGCATCCCCATCCTTCGCGTACGACTCCGATTGCCTCGCCGTCGCAGCGTTAA	CSPTHPHPSRTTPIASPSQR*	-1.35	0.042117	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25664	HRWKVPVR			8	SLAY-screened peptide P4014			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCGGTGGAAGGTGCCGGTGCGTTAGATCTCCAGGGCTAGTTGTTATGCGCATACCTATTAA	HRWKVPVR*ISRASCYAHTY*	-1.35	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25665	PNLCDPWFYRIGVRLPMYSL			20	SLAY-screened peptide P4015			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAATCTTTGCGACCCGTGGTTTTATAGGATTGGGGTTAGGTTGCCTATGTACAGCCTTTAA	PNLCDPWFYRIGVRLPMYSL*	-1.35	0.00328	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25666	SAHTTNMTCPVFD			13	SLAY-screened peptide P4016			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGCCCATACTACCAATATGACCTGCCCGGTCTTTGACTAGGTCGCGACCCAGTTCAGGTAA	SAHTTNMTCPVFD*VATQFR*	-1.349	0.009093	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25667	SMPITIRSNLTQFNNH			16	SLAY-screened peptide P4017			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTATGCCTATCACTATTCGTAGTAACCTCACTCAGTTCAACAATCACTAGGCGCACTGGTAA	SMPITIRSNLTQFNNH*AHW*	-1.349	0.000123	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25668	PPILTRLPRLSVWSVITTMGN			21	SLAY-screened peptide P4018			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCGATACTGACTCGGTTACCCCGTTTATCCGTATGGTCTGTTATAACGACCATGGGTAAC	PPILTRLPRLSVWSVITTMGN	-1.349	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25669	HRNSVLIFTHAASLVLFKLGS			21	SLAY-screened peptide P4019			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCGTAATTCGGTTCTCATTTTTACCCACGCCGCTAGTCTGGTGCTCTTCAAGCTCGGGAGT	HRNSVLIFTHAASLVLFKLGS	-1.349	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25670	MYYSPHHHCHIYTP			14	SLAY-screened peptide P4020			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTATTATTCGCCTCACCATCACTGTCATATCTATACGCCTTAGTTTCCTTCTGCTCCCTAA	MYYSPHHHCHIYTP*FPSAP*	-1.348	0.018861	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25671	PIQRCP			6	SLAY-screened peptide P4021			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATTCAGCGTTGTCCCTAGCTTTACGTTACTGTTATTTCTAGTTATCCCACGTGCCACTAA	PIQRCP*LYVTVISSYPTCH*	-1.348	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25672	DTIRSMLNELNAD			13	SLAY-screened peptide P4022			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACACCATCAGGAGCATGTTGAACGAGCTCAACGCTGATTAGCTGACTCACGGCAGTCGTTAA	DTIRSMLNELNAD*LTHGSR*	-1.348	8.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25673	CTHDEANSQSSAYQFLDYTFN			21	SLAY-screened peptide P4023			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCACTCACGATGAGGCTAACTCCCAGTCCTCTGCCTATCAGTTTCTTGATTATACTTTTAAC	CTHDEANSQSSAYQFLDYTFN	-1.347	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25674	LYMPITPSSAWFMSKDFHPP			20	SLAY-screened peptide P4024			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTATATGCCGATCACTCCCTCGTCCGCCTGGTTTATGTCGAAGGATTTTCATCCCCCGTAA	LYMPITPSSAWFMSKDFHPP*	-1.347	4.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25675	CPVQPLEKPLGQASTPTYVV			20	SLAY-screened peptide P4025			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCTGTTCAGCCGCTCGAGAAGCCCCTGGGGCAGGCGTCTACTCCCACCTATGTTGTTTAA	CPVQPLEKPLGQASTPTYVV*	-1.347	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25676	EQLRSRPGLSCTFSVCVLTN			20	SLAY-screened peptide P4026			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCAGCTGCGGAGCCGCCCCGGGCTTTCCTGCACCTTTTCCGTGTGTGTTCTGACGAACTAA	EQLRSRPGLSCTFSVCVLTN*	-1.347	6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25677	HTSDIYFICHPCNTYFHMNR			20	SLAY-screened peptide P4027			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACCAGTGATATCTACTTTATTTGCCACCCCTGCAATACCTATTTCCACATGAACCGCTAA	HTSDIYFICHPCNTYFHMNR*	-1.347	0.000198	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25678	GTELHFLVACTFDPGYECSS			20	SLAY-screened peptide P4028			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTACTGAGCTGCACTTCCTGGTTGCGTGTACGTTTGATCCCGGGTATGAGTGCTCCAGTTAA	GTELHFLVACTFDPGYECSS*	-1.347	0.00445	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25679	HPRHSINHALLINNTVCLQA			20	SLAY-screened peptide P4029			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCCCGCCATTCTATCAACCACGCGCTTCTCATCAATAATACTGTTTGTTTGCAGGCTTAA	HPRHSINHALLINNTVCLQA*	-1.347	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25680	DVGGLSLHRRFREC			14	SLAY-screened peptide P4030			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGTCGGTGGGCTGTCTCTCCACCGGCGTTTTCGTGAGTGTTAGAGTAACACTGGCACTTAA	DVGGLSLHRRFREC*SNTGT*	-1.346	0.000655	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25681	YTKSLMLVTPILPRDYDTNP			20	SLAY-screened peptide P4031			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACTAAGTCGCTGATGCTTGTCACCCCTATTCTCCCTCGCGACTACGATACCAATCCGTAA	YTKSLMLVTPILPRDYDTNP*	-1.346	2.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25682	TTPTGRSTDVKTGEYVSNAM			20	SLAY-screened peptide P4032			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTACCCCTACGGGGCGTAGCACTGACGTCAAGACTGGTGAGTATGTTAGCAACGCGATGTAA	TTPTGRSTDVKTGEYVSNAM*	-1.346	3.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25683	ADCSSIRYPETFGFAPTFICT			21	SLAY-screened peptide P4033			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGACTGTAGTTCTATCCGGTATCCTGAAACATTTGGCTTTGCTCCGACATTTATCTGTACT	ADCSSIRYPETFGFAPTFICT	-1.346	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25684	HRNTRPSPCFIVYLPPNDAN			20	SLAY-screened peptide P4034			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAGGAATACCCGGCCTTCTCCTTGTTTCATTGTGTACCTCCCGCCCAACGATGCTAATTAA	HRNTRPSPCFIVYLPPNDAN*	-1.345	0.000525	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25685	TLRCSYNSQTWSKA			14	SLAY-screened peptide P4035			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTGCGCTGCTCTTATAACAGCCAGACTTGGAGTAAGGCCTAGCTCTCGTATTTTGACTAA	TLRCSYNSQTWSKA*LSYFD*	-1.345	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25686	GRNHTFYKPNNGTQCINDHS			20	SLAY-screened peptide P4036			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCGTAACCATACTTTCTACAAGCCCAACAATGGTACCCAGTGCATTAACGACCACAGCTAA	GRNHTFYKPNNGTQCINDHS*	-1.345	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25687	PCSPPCTCFTLDFSDCYCLT			20	SLAY-screened peptide P4037			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGCTCGCCCCCCTGCACGTGCTTTACTCTCGATTTTTCTGATTGCTATTGCCTTACTTAA	PCSPPCTCFTLDFSDCYCLT*	-1.345	0.012377	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25688	PTKILTPTMRKNVTHLIANN			20	SLAY-screened peptide P4038			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACGAAGATCTTGACCCCTACGATGCGTAAGAATGTGACGCATCTCATTGCTAACAATTAA	PTKILTPTMRKNVTHLIANN*	-1.345	0.000908	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25689	FDDASRICPIFPSVSSSTTCN			21	SLAY-screened peptide P4039			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGATGATGCCTCCCGGATTTGTCCGATATTCCCATCCGTATCAAGTTCAACTACCTGTAAC	FDDASRICPIFPSVSSSTTCN	-1.345	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25690	FLPPVPSPTYHFFSLPISSI			20	SLAY-screened peptide P4040			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCTGCCCCCTGTCCCCAGTCCTACTTATCACTTTTTTAGCCTTCCCATTTCGTCCATTTAA	FLPPVPSPTYHFFSLPISSI*	-1.344	0.013318	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25691	TNLFCRPVVNIFQTYVCYNL			20	SLAY-screened peptide P4041			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAACCTCTTCTGTAGGCCCGTGGTGAACATCTTCCAGACCTATGTCTGTTATAACCTGTAA	TNLFCRPVVNIFQTYVCYNL*	-1.344	0.025038	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25692	PYMHHDFLHGHTFS			14	SLAY-screened peptide P4042			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTATATGCATCATGACTTTCTTCATGGCCATACGTTTTCCTAGTTCTTGAGCGTTCATTAA	PYMHHDFLHGHTFS*FLSVH*	-1.344	0.005179	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25693	LKTNSDLRPTKLRSYFYLLH			20	SLAY-screened peptide P4043			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGAAGACCAATAGCGACCTTAGGCCGACCAAGCTTAGGTCCTATTTTTATCTTCTGCATTAA	LKTNSDLRPTKLRSYFYLLH*	-1.344	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25694	PLTCPAILVRPSFLAPCYGF			20	SLAY-screened peptide P4044			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCACGTGTCCTGCCATTTTGGTTCGGCCTAGCTTCCTGGCGCCTTGTTATGGCTTTTAA	PLTCPAILVRPSFLAPCYGF*	-1.344	0.02358	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25695	SPWPNDKFLPTTMQPKDARF			20	SLAY-screened peptide P4045			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCCCTGGCCCAACGACAAGTTCCTGCCTACTACTATGCAGCCTAAGGACGCCAGGTTTTAA	SPWPNDKFLPTTMQPKDARF*	-1.343	0.003325	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25696	LNCLQYL			7	SLAY-screened peptide P4046			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAATTGCTTGCAGTACCTCTAGCCCTTTCTTAGGCTTGCCAGGATTTTTCGTCCGCCCTAA	LNCLQYL*PFLRLARIFRPP*	-1.343	3.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25697	SADPHTLVLDINFNSTNMEA			20	SLAY-screened peptide P4047			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGCTGACCCGCACACGCTCGTTTTGGACATCAATTTTAACAGTACCAATATGGAGGCTTAA	SADPHTLVLDINFNSTNMEA*	-1.343	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25698	KRIVLRPSPKWVVEYPPRGD			20	SLAY-screened peptide P4048			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCGTATCGTGCTCCGCCCGTCGCCGAAGTGGGTCGTTGAGTATCCCCCGCGCGGCGACTAA	KRIVLRPSPKWVVEYPPRGD*	-1.343	0.000129	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25699	PERLTPFASSSTYGVVILVN			20	SLAY-screened peptide P4049			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGAGCGCCTCACTCCCTTTGCCAGCTCCAGTACCTACGGCGTCGTCATTCTCGTTAATTAA	PERLTPFASSSTYGVVILVN*	-1.343	0.008537	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25700	RCPRPVYPVDLMLVMSDVFI			20	SLAY-screened peptide P4050			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGCCCGCGGCCGGTGTATCCGGTTGATCTTATGCTGGTTATGTCTGATGTCTTTATCTAA	RCPRPVYPVDLMLVMSDVFI*	-1.342	0.003416	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25701	YADIVSTHIWNSLGERRFAK			20	SLAY-screened peptide P4051			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGCCGACATCGTTTCTACTCATATTTGGAATAGCCTGGGGGAGCGTCGTTTTGCCAAGTAA	YADIVSTHIWNSLGERRFAK*	-1.342	0.049564	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25702	VRPSPFFYFSRYPCGATPYLY			21	SLAY-screened peptide P4052			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCGTCCTTCCCCTTTCTTCTACTTCTCCCGCTATCCTTGTGGTGCTACTCCGTATCTCTAC	VRPSPFFYFSRYPCGATPYLY	-1.342	2.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25703	TLFYITHGHTLKRAYWYNYI			20	SLAY-screened peptide P4053			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCTCTTCTACATCACTCACGGTCATACCCTGAAGCGGGCCTATTGGTATAATTACATCTAA	TLFYITHGHTLKRAYWYNYI*	-1.342	0.00204	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25704	LLCIPVFSA			9	SLAY-screened peptide P4054			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCTCTGCATTCCTGTTTTCTCGGCGTAGTAGAGCCTGAATGATTCGTATAAGTCGTTCTAA	LLCIPVFSA**SLNDSYKSF*	-1.342	0.017316	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25705	IN			2	SLAY-screened peptide P4055			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAACTAGACTTGTCCGCATTCTGTTCCCCAGAGCAGTAAGACTCCCCACGCGCGCCATTAA	IN*TCPHSVPQSSKTPHARH*	-1.341	0.01835	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25706	RRPVLMRVLKAITPYFRW			18	SLAY-screened peptide P4056			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCGTCCTGTCCTTATGCGTGTGTTGAAGGCTATCACTCCTTATTTTCGGTGGTAGGCTTAA	RRPVLMRVLKAITPYFRW*A*	-1.341	3.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25707	FPDSLIGTFCPIVPVPL			17	SLAY-screened peptide P4057			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCCCGATTCGTTAATCGGCACCTTCTGCCCCATAGTTCCCGTGCCTTTATGAAGTCGTAAC	FPDSLIGTFCPIVPVPL*SRN	-1.341	6.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25708	PTFPPQHSSASLSFGGGSPHV			21	SLAY-screened peptide P4058			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACGTTTCCCCCTCAGCACAGTTCGGCCTCCTTGTCATTCGGCGGCGGATCACCACACGTA	PTFPPQHSSASLSFGGGSPHV	-1.341	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25709	PTSIPVTSPHIVEPSCAPRL			20	SLAY-screened peptide P4059			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACTTCTATCCCCGTGACGAGCCCTCACATCGTCGAGCCGAGTTGTGCTCCGCGCTTGTAA	PTSIPVTSPHIVEPSCAPRL*	-1.341	0.007374	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25710	HHSMYPYYPLYSRPVFTYTI			20	SLAY-screened peptide P4060			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCATAGCATGTACCCCTATTATCCTCTCTATAGTCGTCCTGTCTTTACCTATACTATTTAA	HHSMYPYYPLYSRPVFTYTI*	-1.341	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25711	YDYGFHILVYNNEVRGNDRN			20	SLAY-screened peptide P4061			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGATTACGGTTTCCACATCCTCGTTTACAATAACGAGGTTAGGGGTAACGACCGTAACTAA	YDYGFHILVYNNEVRGNDRN*	-1.34	0.000946	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25712	ITRRHALPMIRDSPNVGADD			20	SLAY-screened peptide P4062			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCACTCGCCGGCACGCTTTGCCGATGATCAGGGATAGTCCTAACGTCGGGGCTGATGACTAA	ITRRHALPMIRDSPNVGADD*	-1.34	0.002416	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25713	PPACTPPILCGAPAFCTQVV			20	SLAY-screened peptide P4063			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCGCCTGTACTCCGCCGATTCTTTGCGGTGCCCCTGCGTTTTGCACTCAGGTGGTCTAA	PPACTPPILCGAPAFCTQVV*	-1.34	0.001739	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25714	REASLMFCFVFHCTDFPSTC			20	SLAY-screened peptide P4064			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGGAGGCCAGCCTCATGTTTTGCTTTGTCTTTCACTGCACTGACTTCCCCTCGACGTGCTAA	REASLMFCFVFHCTDFPSTC*	-1.34	0.000788	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25715	HPRTRFRSDIDRRCRRRVTS			20	SLAY-screened peptide P4065			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCGCGCACCCGCTTTCGTTCCGATATTGATCGCCGGTGTCGCCGGCGGGTGACGAGTTAA	HPRTRFRSDIDRRCRRRVTS*	-1.339	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25716	SYPQMHANPYYSIYIIPLVM			20	SLAY-screened peptide P4066			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTATCCTCAGATGCATGCTAATCCTTATTATAGCATTTATATCATTCCTCTTGTGATGTAA	SYPQMHANPYYSIYIIPLVM*	-1.338	0.01141	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25717	AFHCFRPEDYHFHEIYSGHR			20	SLAY-screened peptide P4067			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTTCCACTGCTTTCGCCCGGAGGACTACCATTTCCACGAGATTTATTCCGGCCATCGTTAA	AFHCFRPEDYHFHEIYSGHR*	-1.338	0.04112	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25718	WRYNAPMDGGLHNIIL			16	SLAY-screened peptide P4068			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCGTTACAATGCCCCGATGGATGGTGGCCTCCACAACATTATTTTGTAGTTCATGACCTAA	WRYNAPMDGGLHNIIL*FMT*	-1.338	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25719	EQKSLATPDVTDTCLRYKIV			20	SLAY-screened peptide P4069			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCAGAAGAGCTTGGCGACCCCCGACGTTACCGATACCTGTCTCCGCTATAAGATCGTGTAA	EQKSLATPDVTDTCLRYKIV*	-1.338	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25720	TSRRHRCSRRTGHMVNFSHVN			21	SLAY-screened peptide P4070			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAGCCGCCGGCATCGGTGTTCGCGTCGGACCGGGCACATGGTGAACTTTTCCCATGTTAAC	TSRRHRCSRRTGHMVNFSHVN	-1.338	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25721	PCTMPPLSDFKAIGRTSTEP			20	SLAY-screened peptide P4071			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGTACGATGCCCCCCTTGTCGGATTTCAAGGCCATTGGCCGCACTTCGACTGAGCCCTAA	PCTMPPLSDFKAIGRTSTEP*	-1.338	0.003683	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25722	SCTSADSLIWLVLNRVPPSIY			21	SLAY-screened peptide P4072			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTGCACGAGTGCTGACTCGCTTATCTGGCTCGTTCTTAACCGTGTCCCTCCGTCTATTTAC	SCTSADSLIWLVLNRVPPSIY	-1.338	0.004231	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25723	ETSQRFQSIFRCYPL			15	SLAY-screened peptide P4073			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGACTTCGCAGCGGTTCCAGTCTATTTTTCGTTGTTATCCTCTTTAGCCCACTATTTTCTAA	ETSQRFQSIFRCYPL*PTIF*	-1.338	0.04895	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25724	PYLPDGSHSQPCALYSIPWV			20	SLAY-screened peptide P4074			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTATCTGCCCGACGGCTCTCACTCCCAGCCGTGTGCCCTCTACTCGATTCCTTGGGTGTAA	PYLPDGSHSQPCALYSIPWV*	-1.337	0.000213	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25725	HSFPISLCHWLRMKFKSCHS			20	SLAY-screened peptide P4075			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAGCTTCCCGATTAGCCTTTGTCACTGGCTGAGGATGAAGTTCAAGTCTTGTCACTCCTAA	HSFPISLCHWLRMKFKSCHS*	-1.337	0.000865	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25726	PWLFDTPNDCLRPSSIDTIA			20	SLAY-screened peptide P4076			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGGCTGTTTGACACTCCTAACGATTGCTTGCGCCCCTCCTCCATCGACACTATCGCTTAA	PWLFDTPNDCLRPSSIDTIA*	-1.337	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25727	ARLTAITHPMPHWH			14	SLAY-screened peptide P4077			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCGTCTGACTGCTATTACCCATCCGATGCCGCACTGGCACTAGTCCGCTTACCGTGATTAA	ARLTAITHPMPHWH*SAYRD*	-1.337	0.001647	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25728	HKPLGSSARLSVSPLPLIMAN			21	SLAY-screened peptide P4078			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAAGCCCCTCGGTAGTTCTGCACGCCTATCAGTAAGCCCTCTTCCGCTTATAATGGCTAAC	HKPLGSSARLSVSPLPLIMAN	-1.337	0.00157	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25729	RPFQVASGLLVFLCSAIRTTN			21	SLAY-screened peptide P4079			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCCTTTCAGGTAGCAAGCGGCCTGCTAGTATTCCTCTGTTCGGCCATCAGGACAACTAAC	RPFQVASGLLVFLCSAIRTTN	-1.336	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25730	KSTVLKNPTCL			11	SLAY-screened peptide P4080			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGTCTACTGTCTTGAAGAACCCCACCTGCCTCTAGTCTGTCACCACGTGGACCTTTTTTTAA	KSTVLKNPTCL*SVTTWTFF*	-1.336	0.000134	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25731	YLCSRKLCYCDILHVQNIRP			20	SLAY-screened peptide P4081			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCTTTGCAGCCGCAAGCTGTGTTACTGCGATATCCTCCACGTCCAGAACATTCGTCCTTAA	YLCSRKLCYCDILHVQNIRP*	-1.336	5.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25732	LRHLLCFNARTVLKPLITNN			20	SLAY-screened peptide P4082			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCGCCACCTTCTCTGCTTTAACGCCCGCACGGTTCTTAAGCCTTTGATTACCAACAATTAA	LRHLLCFNARTVLKPLITNN*	-1.336	0.039261	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25733	PQKSSFSNAWHRLADLRRRL			20	SLAY-screened peptide P4083			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCAGAAGTCTTCCTTTTCCAATGCGTGGCACCGCTTGGCTGACTTGCGTCGGCGCTTGTAA	PQKSSFSNAWHRLADLRRRL*	-1.335	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25734	HLCPRDIHKNILKTLYSEYF			20	SLAY-screened peptide P4084			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCTCTGTCCCCGTGATATTCATAAGAATATTCTGAAGACTCTGTACTCTGAGTATTTCTAA	HLCPRDIHKNILKTLYSEYF*	-1.335	4.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25735	FSPSTLIITNYVFNTRLPLL			20	SLAY-screened peptide P4085			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTCCCCGTCTACTCTGATCATTACGAATTACGTTTTCAATACTCGTTTGCCTCTTCTCTAA	FSPSTLIITNYVFNTRLPLL*	-1.335	0.047667	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25736	LYRRSIPDALLMNKTQLTGG			20	SLAY-screened peptide P4086			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTATCGCCGGTCCATTCCTGACGCCCTTCTCATGAACAAGACGCAGTTGACTGGTGGTTAA	LYRRSIPDALLMNKTQLTGG*	-1.334	0.003359	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25737	SYNCGIMTTLVNDWQPTQNH			20	SLAY-screened peptide P4087			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTACAATTGCGGCATTATGACCACTCTTGTGAATGATTGGCAGCCTACTCAGAACCACTAA	SYNCGIMTTLVNDWQPTQNH*	-1.334	0.013013	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25738	LRSHLYIYTHTRPMYPDDPA			20	SLAY-screened peptide P4088			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCGCTCGCACCTTTACATTTATACTCACACCCGTCCCATGTACCCGGACGATCCCGCCTAA	LRSHLYIYTHTRPMYPDDPA*	-1.334	0.004469	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25739	SPSSKYTPILSMIWHNIRTPN			21	SLAY-screened peptide P4089			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCCTTCCAGTAAGTATACTCCGATCCTCAGCATGATCTGGCACAACATTAGAACACCTAAC	SPSSKYTPILSMIWHNIRTPN	-1.334	0.01794	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25740	LCSHYATLLPIISTMRGRFY			20	SLAY-screened peptide P4090			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTGTTCTCACTATGCGACTCTCCTCCCTATCATCTCTACCATGCGTGGTCGGTTTTATTAA	LCSHYATLLPIISTMRGRFY*	-1.334	0.000209	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25741	FSVWPRSLNDSVYSNISSII			20	SLAY-screened peptide P4091			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTCCGTGTGGCCTCGCTCCCTCAACGACTCTGTCTATTCTAATATTAGTTCTATCATTTAA	FSVWPRSLNDSVYSNISSII*	-1.334	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25742	TCESPIRRILMVLTKRIRKL			20	SLAY-screened peptide P4092			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTGCGAGTCGCCCATCCGGAGGATTCTGATGGTCCTTACTAAGCGGATCCGCAAGCTCTAA	TCESPIRRILMVLTKRIRKL*	-1.334	0.02888	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25743	YLVLLFLVYFYVPIPMTDYRN			21	SLAY-screened peptide P4093			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCTGGTTCTTCTTTTCCTTGTTTATTTTTATGTTCCCATCCCTATGACTGATTACCGTAAC	YLVLLFLVYFYVPIPMTDYRN	-1.334	0.001169	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25744	TNQRTFTIFLNVANW			15	SLAY-screened peptide P4094			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAACCAGCGCACGTTTACTATCTTCCTCAATGTCGCGAACTGGTAGAGTAGTTAGGGTTAA	TNQRTFTIFLNVANW*SS*G*	-1.333	6.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25745	RSM			3	SLAY-screened peptide P4095			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTCGATGTAGCGTATTCCCAGTCGCCCTATACCCCTTGGAGCCATCCTCTCCCTTAGTAAC	RSM*RIPSRPIPLGAILSLSN	-1.333	0.000109	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25746	PPFVSPHLRSASLRLSSSET			20	SLAY-screened peptide P4096			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCTTTCGTCTCCCCTCACCTCCGCTCCGCCAGCCTGCGCCTCAGTTCTTCCGAGACTTAA	PPFVSPHLRSASLRLSSSET*	-1.333	0.004529	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25747	FTIRLLVTTFIIVYVRRSST			20	SLAY-screened peptide P4097			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTACTATCCGTCTTCTTGTCACCACTTTTATCATTGTGTATGTCCGCCGGTCCAGTACCTAA	FTIRLLVTTFIIVYVRRSST*	-1.333	0.000395	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25748	LHAWKAHISANRTRFLHTEP			20	SLAY-screened peptide P4098			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCATGCTTGGAAGGCCCATATCAGCGCTAATAGGACGCGCTTTCTGCATACTGAGCCCTAA	LHAWKAHISANRTRFLHTEP*	-1.333	0.005406	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25749	SSNPLIRTRCLHGRLIILL			19	SLAY-screened peptide P4099			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTCGAACCCTCTCATCCGCACCCGTTGCCTCCACGGTCGCCTTATTATCCTGCTCTAGTAA	SSNPLIRTRCLHGRLIILL**	-1.333	0.001505	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25750	SCSHELRQSSPYSLYGTRMA			20	SLAY-screened peptide P4100			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTGCTCGCACGAGCTCCGCCAGTCCAGCCCGTACTCCCTGTATGGCACTCGCATGGCGTAA	SCSHELRQSSPYSLYGTRMA*	-1.332	0.001639	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25751	RCHAGTLIWPYQTIYMH			17	SLAY-screened peptide P4101			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTGTCACGCCGGCACTCTCATCTGGCCTTACCAGACGATCTACATGCACTAGAACTACTAA	RCHAGTLIWPYQTIYMH*NY*	-1.332	0.024837	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25752	LYPLTGASYYVTKALGISAL			20	SLAY-screened peptide P4102			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTACCCTCTCACGGGTGCCTCTTACTATGTTACCAAGGCGCTGGGCATTAGTGCCCTCTAA	LYPLTGASYYVTKALGISAL*	-1.331	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25753	VCFREPPPPWRHLKYIHYKR			20	SLAY-screened peptide P4103			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTGTTTCAGGGAGCCCCCCCCTCCGTGGCGTCACCTGAAGTACATTCACTACAAGAGGTAA	VCFREPPPPWRHLKYIHYKR*	-1.331	5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25754	NCIHFLTVYNVDKYNYGINS			20	SLAY-screened peptide P4104			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTGTATTCACTTCCTCACGGTCTATAATGTTGACAAGTACAACTATGGTATCAACAGTTAA	NCIHFLTVYNVDKYNYGINS*	-1.331	0.006868	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25755	PNR			3	SLAY-screened peptide P4105			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAACAGGTAGGGTCCCAGCTGCCTCACTCGCGTGGATTCTACTATCAGCGCTGTGCTTTAA	PNR*GPSCLTRVDSTISAVL*	-1.331	7.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25756	TN			2	SLAY-screened peptide P4106			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGAATTAGTTTGGTGGCCGGTACGCCAAGATCATTCATTGTCGGGCCTGTGCGTTTTTCTAA	TN*FGGRYAKIIHCRACAFF*	-1.331	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25757	YHDDCDPVTMSMVFFRMRAY			20	SLAY-screened peptide P4107			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCATGATGACTGCGACCCGGTTACTATGTCGATGGTTTTCTTCCGCATGCGCGCCTACTAA	YHDDCDPVTMSMVFFRMRAY*	-1.331	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25758	KCPQSSGDSTLVKNTKWQTT			20	SLAY-screened peptide P4108			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGTGTCCTCAGTCTAGTGGTGATAGTACGCTGGTCAAGAATACCAAGTGGCAGACGACTTAA	KCPQSSGDSTLVKNTKWQTT*	-1.331	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25759	PNMDALPFWCKAYNSSCGRL			20	SLAY-screened peptide P4109			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAACATGGATGCCTTGCCTTTCTGGTGCAAGGCCTACAACAGTTCGTGCGGTCGTCTGTAA	PNMDALPFWCKAYNSSCGRL*	-1.33	0.008887	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25760	VRMLPVMVDKPLTTYNNART			20	SLAY-screened peptide P4110			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCGCATGCTCCCTGTGATGGTTGATAAGCCGCTCACTACCTACAATAATGCCCGTACTTAA	VRMLPVMVDKPLTTYNNART*	-1.33	0.005029	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25761	STRDDH			6	SLAY-screened peptide P4111			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCACCCGTGACGACCACTAGGTCCGTTGTATTGACCTTTCGTTGCCGCTCGACATCCACTAA	STRDDH*VRCIDLSLPLDIH*	-1.33	0.003227	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25762	NLIQPALHSYCNYVPIRSFR			20	SLAY-screened peptide P4112			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCTCATTCAGCCGGCTCTGCACTCTTATTGCAACTACGTTCCCATTCGCAGCTTTAGGTAA	NLIQPALHSYCNYVPIRSFR*	-1.33	0.000484	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25763	YTFLRFDYAPYSPQSRFFSV			20	SLAY-screened peptide P4113			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACGTTCCTCCGTTTTGACTACGCGCCGTACAGCCCTCAGTCCCGGTTTTTTTCCGTCTAA	YTFLRFDYAPYSPQSRFFSV*	-1.33	0.030304	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25764	SRMQRPHTSNSSGPKSSNWP			20	SLAY-screened peptide P4114			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCGCATGCAGCGCCCTCATACTAGCAATAGTAGTGGCCCGAAGAGCTCTAACTGGCCCTAA	SRMQRPHTSNSSGPKSSNWP*	-1.33	0.002301	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25765	TFRCMLHDRSHT			12	SLAY-screened peptide P4115			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTTCCGCTGTATGCTGCACGACCGGAGTCATACTTAGGCCGATCGCTACATGGTCCCTTAA	TFRCMLHDRSHT*ADRYMVP*	-1.33	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25766	RTIG			4	SLAY-screened peptide P4116			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACCATCGGGTAGCACGCCTTGCCTACGATCCTCATGCTCACCGCTACTGCTAATTAGTAA	RTIG*HALPTILMLTATAN**	-1.329	0.032709	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25767	HLSWLLNFYKLIFAIIPASR			20	SLAY-screened peptide P4117			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCTTAGTTGGTTGCTGAATTTCTACAAGCTTATTTTTGCGATTATCCCTGCTTCGAGGTAA	HLSWLLNFYKLIFAIIPASR*	-1.329	7.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25768	GNSRRTMHSNCCLWHYDCLV			20	SLAY-screened peptide P4118			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCAATTCCAGGAGGACCATGCATTCCAATTGCTGTCTCTGGCATTACGATTGTCTCGTGTAA	GNSRRTMHSNCCLWHYDCLV*	-1.328	0.00063	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25769	PRHLPSPHLHCANSNFSFVP			20	SLAY-screened peptide P4119			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGGCATCTTCCGTCTCCGCACCTGCACTGCGCGAACTCTAATTTCTCTTTCGTCCCGTAA	PRHLPSPHLHCANSNFSFVP*	-1.328	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25770	YHPECQYLSVPVNVIPTDMP			20	SLAY-screened peptide P4120			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCATCCTGAGTGCCAGTACCTGTCCGTGCCCGTTAACGTCATTCCGACGGATATGCCCTAA	YHPECQYLSVPVNVIPTDMP*	-1.328	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25771	SHRRFLGNNDYYNLHTFAFN			20	SLAY-screened peptide P4121			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCATAGGCGGTTCCTCGGGAACAACGATTACTACAATCTGCACACCTTTGCCTTTAACTAA	SHRRFLGNNDYYNLHTFAFN*	-1.328	5.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25772	TIRKSLAALFLLIAGCPPAV			20	SLAY-screened peptide P4122			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTATCCGCAAGTCGTTGGCGGCCCTGTTCCTGCTTATTGCGGGGTGCCCGCCCGCCGTCTAA	TIRKSLAALFLLIAGCPPAV*	-1.328	7.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25773	HAMLA			5	SLAY-screened peptide P4123			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGCTATGCTGGCTTAGGCCTGGCGGGCGTTCAATTTAGCAATATGCGGCGCAAGCCCTAAC	HAMLA*AWRAFNLAICGASPN	-1.328	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25774	TDTPTPRHAHTHVNLH			16	SLAY-screened peptide P4124			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGACACCCCGACCCCCCGCCATGCCCACACCCACGTTAATTTGCATTAGACCCCGGGTTAA	TDTPTPRHAHTHVNLH*TPG*	-1.328	0.002956	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25775	DHNFYFYIMLDNAPPIR			17	SLAY-screened peptide P4125			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCATAATTTCTATTTCTACATTATGCTTGACAATGCTCCTCCCATCAGGTAGTCCCATTAA	DHNFYFYIMLDNAPPIR*SH*	-1.328	0.00016	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25776	SDSLSRSIVLCIIICLSVN			19	SLAY-screened peptide P4126			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGACAGCCTCAGTCGATCGATAGTACTTTGCATTATAATATGCCTTAGTGTTAACTGAGTA	SDSLSRSIVLCIIICLSVN*V	-1.327	0.010136	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25777	IY			2	SLAY-screened peptide P4127			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTACTAGTTCAGTCCCCCTCGGAATGCCTAGCGGATTCCCTAGAAGATTCAGATTCACTAA	IY*FSPPRNA*RIP*KIQIH*	-1.327	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25778	DSVLMRINHLATCPCMPDVH			20	SLAY-screened peptide P4128			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACAGTGTTTTGATGCGGATCAATCATCTTGCTACGTGCCCGTGCATGCCTGATGTCCATTAA	DSVLMRINHLATCPCMPDVH*	-1.327	0.046413	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25779	PQRRIDVMLNRFPFGRPNLE			20	SLAY-screened peptide P4129			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCAGCGGCGTATCGACGTGATGTTGAACCGGTTCCCGTTTGGCCGTCCGAACCTTGAGTAA	PQRRIDVMLNRFPFGRPNLE*	-1.327	0.014792	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25780	PNYTHSYRYKCVWCVIIGLGN			21	SLAY-screened peptide P4130			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAACTATACTCACAGTTACCGGTATAAGTGCGTTTGGTGCGTTATTATAGGACTAGGTAAC	PNYTHSYRYKCVWCVIIGLGN	-1.327	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25781	HRPHMMGFTVPYERHFYALR			20	SLAY-screened peptide P4131			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAGGCCTCACATGATGGGCTTTACTGTTCCGTACGAGAGGCATTTTTATGCGCTCCGCTAA	HRPHMMGFTVPYERHFYALR*	-1.327	0.029165	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25782	APLSSYLIARTRTIWTFGGQ			20	SLAY-screened peptide P4132			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCGCTTAGTAGTTATCTTATTGCTAGGACCCGTACGATTTGGACCTTCGGCGGTCAGTAA	APLSSYLIARTRTIWTFGGQ*	-1.326	0.000489	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25783	TSYQPHESYPFGCSPFTFAA			20	SLAY-screened peptide P4133			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCGTATCAGCCTCACGAGAGTTATCCTTTCGGGTGTTCCCCCTTTACCTTTGCCGCGTAA	TSYQPHESYPFGCSPFTFAA*	-1.326	0.001725	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25784	QTIRFRGRNSDITFVNP			17	SLAY-screened peptide P4134			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGACCATCCGGTTTCGGGGCCGCAACTCGGACATCACCTTTGTGAATCCTTAAGAATTAACT	QTIRFRGRNSDITFVNP*ELT	-1.325	0.014355	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25785	ASGVPNIVPVSSPVLHCSY			19	SLAY-screened peptide P4135			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTCTGGCGTGCCTAACATTGTTCCTGTCTCGAGTCCGGTTCTGCACTGTAGCTATTGAGTA	ASGVPNIVPVSSPVLHCSY*V	-1.325	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25786	GTRPDAALFHKCHTVDYSMA			20	SLAY-screened peptide P4136			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCACTCGTCCTGATGCCGCCCTTTTCCACAAGTGTCATACTGTTGACTATAGTATGGCTTAA	GTRPDAALFHKCHTVDYSMA*	-1.325	0.002477	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25787	VQIRLIMLTLRRLLLRLSVFN			21	SLAY-screened peptide P4137			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCAGATTCGTCTAATTATGCTGACATTACGTAGATTACTGCTGCGCTTAAGTGTTTTTAAC	VQIRLIMLTLRRLLLRLSVFN	-1.325	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25788	RIHRRSHLDHVLN			13	SLAY-screened peptide P4138			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTATTCACCGTAGGTCTCATCTTGACCACGTTCTTAACTAGAATTCTCGCTGCCTTGATTAA	RIHRRSHLDHVLN*NSRCLD*	-1.324	0.048346	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25789	CVPYMTIADLSIHHILYPLV			20	SLAY-screened peptide P4139			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGTCCCGTACATGACTATCGCCGACTTGAGCATTCACCACATCCTCTATCCTCTCGTTTAA	CVPYMTIADLSIHHILYPLV*	-1.324	0.04407	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25790	CYHWPHDFLYTRADHTPNFS			20	SLAY-screened peptide P4140			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTACCACTGGCCTCACGATTTTCTGTACACCCGGGCGGACCACACCCCGAACTTTTCCTAA	CYHWPHDFLYTRADHTPNFS*	-1.324	0.03968	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25791	LSLLPAPWLPRIMLSARCPFN			21	SLAY-screened peptide P4141			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTCCCTTCTGCCTGCCCCGTGGCTACCCCGGATCATGCTCTCTGCACGATGCCCCTTTAAC	LSLLPAPWLPRIMLSARCPFN	-1.324	0.013062	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25792	ICLLIQSYGAPRHWSFALQS			20	SLAY-screened peptide P4142			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTGTCTTCTTATTCAGTCCTACGGTGCGCCCAGGCACTGGTCGTTTGCCCTGCAGTCCTAA	ICLLIQSYGAPRHWSFALQS*	-1.323	0.011321	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25793	CMLH			4	SLAY-screened peptide P4143			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTATGCTTCACTAGAATCCGAAGCTGTCGCTTTCCATCACCTGGCTTACGTCTAGCCCCTAA	CMLH*NPKLSLSITWLTSSP*	-1.323	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25794	THTPGTQLVAISHSRLPPSM			20	SLAY-screened peptide P4144			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCACACGCCTGGGACGCAGCTCGTGGCTATCTCGCACAGTCGCTTGCCGCCGAGTATGTAA	THTPGTQLVAISHSRLPPSM*	-1.323	0.000163	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25795	LFFSVRLRSDLTGSNISDCL			20	SLAY-screened peptide P4145			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTCTTCTCCGTCCGGCTTCGCTCCGACCTGACGGGCTCGAATATTAGCGACTGTCTCTAA	LFFSVRLRSDLTGSNISDCL*	-1.323	0.03841	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25796	SHPIRTWLASIVPCLLSYNR			20	SLAY-screened peptide P4146			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCATCCTATTCGTACCTGGCTCGCCAGTATTGTGCCCTGCCTTCTTTCCTATAATCGCTAA	SHPIRTWLASIVPCLLSYNR*	-1.323	0.010005	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25797	LLYRNYTPSLNGLWYCNFPR			20	SLAY-screened peptide P4147			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTGTACCGTAACTACACTCCCTCCCTCAATGGGCTCTGGTACTGTAACTTCCCGCGTTAA	LLYRNYTPSLNGLWYCNFPR*	-1.322	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25798	PLPWTPRC			8	SLAY-screened peptide P4148			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTCCCCTGGACTCCTCGTTGTTAGTTCACGAGCAACTTGGCTTACAACAATATCCCCTAA	PLPWTPRC*FTSNLAYNNIP*	-1.322	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25799	PAHASNMRTT			10	SLAY-screened peptide P4149			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCCCACGCTTCGAACATGCGCACGACCTAGTTGCCGTCTTCCGCGTCTATTGACACCTAA	PAHASNMRTT*LPSSASIDT*	-1.322	0.012729	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25800	FFASALNRYN			10	SLAY-screened peptide P4150			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTTTGCGAGTGCCCTGAATCGGTACAATTAGCATGCTCTTGTTGTTCCCATTGGGCCGTAA	FFASALNRYN*HALVVPIGP*	-1.321	0.027588	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25801	PWTYCTYGTRNNHWDFFSQF			20	SLAY-screened peptide P4151			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGGACGTACTGTACGTACGGCACGCGGAACAACCACTGGGATTTCTTTTCGCAGTTCTAA	PWTYCTYGTRNNHWDFFSQF*	-1.321	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25802	TSPKAHTVFGNMSRCVPSRA			20	SLAY-screened peptide P4152			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTCTCCGAAGGCGCACACCGTTTTTGGGAACATGAGTCGCTGTGTCCCCAGCAGGGCTTAA	TSPKAHTVFGNMSRCVPSRA*	-1.321	7.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25803	RNRPRNPAVGHSSYVERSHH			20	SLAY-screened peptide P4153			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAACCGGCCTAGGAATCCTGCCGTCGGGCACAGCAGTTACGTTGAGCGGTCCCATCATTAA	RNRPRNPAVGHSSYVERSHH*	-1.321	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25804	YFGPFGLPLFRWRRYPRQIN			20	SLAY-screened peptide P4154			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTTTGGTCCTTTTGGTCTTCCCCTTTTCAGGTGGCGGCGCTACCCTAGGCAGATTAACTGA	YFGPFGLPLFRWRRYPRQIN*	-1.321	5.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25805	LLKRLLSYCCHFSPLFRPND			20	SLAY-screened peptide P4155			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCTTAAGCGTTTGCTCAGCTACTGTTGTCATTTCTCCCCCTTGTTTCGTCCCAATGATTAA	LLKRLLSYCCHFSPLFRPND*	-1.32	0.016434	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25806	KPSIAASPPCPDTTSDRRGE			20	SLAY-screened peptide P4156			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCCGAGTATCGCTGCGTCCCCTCCGTGCCCGGATACCACGTCTGATCGTAGGGGCGAGTAA	KPSIAASPPCPDTTSDRRGE*	-1.32	0.008096	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25807	LSPRRSAVPKAKR			13	SLAY-screened peptide P4157			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAGCCCCCGCCGCTCCGCCGTTCCTAAGGCCAAGAGGTAGCTTTAGGCCCCGGTCGACCTG	LSPRRSAVPKAKR*L*APVDL	-1.32	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25808	HGHTAARYNHKSSCRMTFPL			20	SLAY-screened peptide P4158			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGGCCACACGGCGGCCCGTTATAACCATAAGAGCAGCTGTCGTATGACTTTCCCTTTGTAA	HGHTAARYNHKSSCRMTFPL*	-1.319	0.001719	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25809	SPVSYNAPLLRLIERTVVN			19	SLAY-screened peptide P4159			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCCCGTGTCTTACAACGCGCCCCTCCTCCGCCTGATCGAGAGGACCGTCGTTAATTAGTAA	SPVSYNAPLLRLIERTVVN**	-1.319	0.002004	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25810	HPMNGHCGYYNVVPYHSLLV			20	SLAY-screened peptide P4160			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCGATGAATGGGCATTGCGGTTATTACAATGTCGTTCCTTATCATTCTTTGCTGGTGTAA	HPMNGHCGYYNVVPYHSLLV*	-1.319	0.040183	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25811	PPHSPIYQHPSHFAVHRPLLT			21	SLAY-screened peptide P4161			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTCATAGCCCTATCTACCAGCATCCTTCTCATTTCGCTGTGCATCGACCACTGCTAACT	PPHSPIYQHPSHFAVHRPLLT	-1.319	0.01269	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25812	ARTGMLNSLTIHPGSTTDAP			20	SLAY-screened peptide P4162			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCGGACCGGCATGCTGAACTCTCTTACCATTCACCCTGGGTCCACCACTGACGCCCCTTAA	ARTGMLNSLTIHPGSTTDAP*	-1.319	0.004089	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25813	PHCPSFMDRNIMSFFTFVNN			20	SLAY-screened peptide P4163			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCACTGTCCCTCTTTCATGGACCGCAACATCATGTCGTTTTTCACGTTTGTTAATAACTAA	PHCPSFMDRNIMSFFTFVNN*	-1.319	0.011357	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25814	SPSGCEFSDITHRSVTVGLI			20	SLAY-screened peptide P4164			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCCCAGCGGGTGTGAGTTTTCTGATATCACGCATAGGTCTGTTACCGTCGGCCTTATCTAA	SPSGCEFSDITHRSVTVGLI*	-1.319	0.000241	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25815	DVELPNDAHPKCPTIRFTSPN			21	SLAY-screened peptide P4165			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATGTTGAGCTGCCCAATGATGCCCACCCCAAGTGCCCCACAATAAGGTTTACATCGCCTAAC	DVELPNDAHPKCPTIRFTSPN	-1.319	0.000297	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25816	APLSPLGSLYTSTP			14	SLAY-screened peptide P4166			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCCTCTGTCCCCCCTTGGTTCCCTCTACACGAGTACTCCTTAGGCCTTCAAGACTCGCTAA	APLSPLGSLYTSTP*AFKTR*	-1.318	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25817	SDFNEYKSDSRRDLRSSTHK			20	SLAY-screened peptide P4167			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGATTTCAATGAGTATAAGAGTGATTCCCGGCGCGATCTCAGGAGTTCGACCCACAAGTAA	SDFNEYKSDSRRDLRSSTHK*	-1.318	0.046193	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25818	TRSPTHTLSLCALELLTE			18	SLAY-screened peptide P4168			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAGGTCGCCTACGCACACGCTTTCCCTTTGTGCCCTTGAATTATTAACTGAGTAAGTCGAC	TRSPTHTLSLCALELLTE*VD	-1.318	0.022342	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25819	PNCFNFHPYYYRVCNSLLRC			20	SLAY-screened peptide P4169			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAATTGTTTTAATTTTCACCCTTATTACTACCGTGTGTGTAACAGCCTCCTCAGGTGCTAA	PNCFNFHPYYYRVCNSLLRC*	-1.318	0.027808	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25820	FVVNPNHPAVSIPIPSRNRI			20	SLAY-screened peptide P4170			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGTGGTCAATCCTAATCATCCCGCTGTGTCTATCCCTATCCCTTCCCGGAATCGGATTTAA	FVVNPNHPAVSIPIPSRNRI*	-1.318	0.009109	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25821	YWISRLSYFNQPCFYL			16	SLAY-screened peptide P4171			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTGGATTAGTAGGCTTTCTTACTTCAACCAGCCGTGCTTCTACTTGTAGATTTCTCCCTAA	YWISRLSYFNQPCFYL*ISP*	-1.318	0.006636	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25822	CKSWVAYDSHDKSKWDEGIL			20	SLAY-screened peptide P4172			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAAGTCCTGGGTTGCCTACGACTCCCACGATAAGTCTAAGTGGGACGAGGGTATTCTTTAA	CKSWVAYDSHDKSKWDEGIL*	-1.318	0.000984	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25823	LPHCAVPSYRLVGTNYSFPF			20	SLAY-screened peptide P4173			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCCCATTGTGCTGTCCCGTCCTACCGCCTCGTCGGCACTAACTACAGCTTTCCCTTTTAA	LPHCAVPSYRLVGTNYSFPF*	-1.318	2.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25824	DHLPVVTLYSMLSCHSYCLL			20	SLAY-screened peptide P4174			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCATCTGCCTGTGGTCACGCTGTATAGTATGCTCTCCTGCCACTCCTATTGCCTCCTCTAA	DHLPVVTLYSMLSCHSYCLL*	-1.317	0.001614	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25825	TVGHIAHTLITVANTTYYVS			20	SLAY-screened peptide P4175			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTGGGCCATATCGCGCATACCTTGATTACGGTTGCTAACACTACGTATTATGTCTCGTAA	TVGHIAHTLITVANTTYYVS*	-1.317	0.001245	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25826	PTQSGTLNTFHHFVWNTKRS			20	SLAY-screened peptide P4176			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACTCAGAGCGGTACCCTCAATACCTTTCACCACTTTGTCTGGAATACTAAGCGCTCCTAA	PTQSGTLNTFHHFVWNTKRS*	-1.317	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25827	SPDTRMTRPTPLSFYAHLRT			20	SLAY-screened peptide P4177			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCCGGACACGCGGATGACCCGGCCGACCCCTCTGAGTTTCTATGCGCATCTGCGCACCTAA	SPDTRMTRPTPLSFYAHLRT*	-1.317	0.023457	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25828	GKDSCQFCILHFNAIYPYLI			20	SLAY-screened peptide P4178			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCAAGGACTCTTGCCAGTTCTGTATCCTTCACTTTAATGCTATCTACCCGTACCTGATTTAA	GKDSCQFCILHFNAIYPYLI*	-1.317	0.004433	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25829	HHASNSRLLPDSPDSPLCRR			20	SLAY-screened peptide P4179			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCATGCTTCCAACTCCAGGCTTCTCCCCGACTCCCCTGACAGCCCGTTGTGTCGTCGCTAA	HHASNSRLLPDSPDSPLCRR*	-1.317	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25830	CWRRRPYPLKLFWM			14	SLAY-screened peptide P4180			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTGGCGCCGCCGGCCCTACCCTCTCAAGCTCTTTTGGATGTAGCATCGCAGTCCCTTTTAA	CWRRRPYPLKLFWM*HRSPF*	-1.316	0.001285	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25831	TPTYCSTDDCSARRCVIYNI			20	SLAY-screened peptide P4181			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCCCACCTATTGCTCTACCGATGATTGTTCGGCCCGCCGTTGCGTTATTTACAATATCTAA	TPTYCSTDDCSARRCVIYNI*	-1.316	0.003041	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25832	RRFGPRPCTPPPNYMVLVTT			20	SLAY-screened peptide P4182			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGAGGTTTGGCCCGCGCCCTTGCACCCCTCCGCCCAACTACATGGTCCTGGTCACCACGTAA	RRFGPRPCTPPPNYMVLVTT*	-1.316	0.011794	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25833	SCPHTAMDSRFIANRFHAVC			20	SLAY-screened peptide P4183			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTGCCCGCATACGGCGATGGATTCCAGGTTTATTGCCAACCGGTTCCACGCCGTTTGCTAA	SCPHTAMDSRFIANRFHAVC*	-1.315	0.002232	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25834	ALPSIHCARSLHHPATNDSA			20	SLAY-screened peptide P4184			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCTCCCTTCCATTCACTGTGCCCGCAGTCTGCATCATCCGGCTACCAACGACTCCGCCTAA	ALPSIHCARSLHHPATNDSA*	-1.315	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25835	SRNWAHMLSQVNQMKNMRGLT			21	SLAY-screened peptide P4185			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAGGAACTGGGCTCACATGCTCTCGCAGGTTAATCAGATGAAGAATATGAGAGGATTAACT	SRNWAHMLSQVNQMKNMRGLT	-1.314	2.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25836	HAYPTLPHMRVRDNTLHSTL			20	SLAY-screened peptide P4186			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCCTACCCTACCCTGCCCCACATGCGCGTCCGGGACAATACTCTGCACTCTACCCTTTAA	HAYPTLPHMRVRDNTLHSTL*	-1.314	5.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25837	SRDAPGSYSHEGCII			15	SLAY-screened peptide P4187			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCGTGACGCCCCCGGCAGCTACAGCCACGAGGGCTGCATTATTTAGAATGGGGTCTCTTAA	SRDAPGSYSHEGCII*NGVS*	-1.314	0.021424	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25838	RILFVPCYLPYLCCMNDIIV			20	SLAY-screened peptide P4188			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGATTCTCTTTGTCCCTTGCTACCTGCCTTACCTCTGCTGTATGAACGACATTATTGTGTAA	RILFVPCYLPYLCCMNDIIV*	-1.314	0.034187	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25839	ATCGLPYQPFFPYTWNGSYL			20	SLAY-screened peptide P4189			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACTTGCGGTCTGCCCTATCAGCCCTTCTTCCCTTACACCTGGAACGGCTCTTACCTTTAA	ATCGLPYQPFFPYTWNGSYL*	-1.314	0.000355	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25840	PAFHPTATVPWS			12	SLAY-screened peptide P4190			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCCTTTCATCCGACTGCGACTGTGCCCTGGTCCTAGAGTAACACCCGTTTTCGTTAGTAA	PAFHPTATVPWS*SNTRFR**	-1.314	7.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25841	TSISS			5	SLAY-screened peptide P4191			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCTATCTCCTCCTAGAGTATCAATTATCATATTATTTTTTGGTACTCCGGTTTGATCTAA	TSISS*SINYHIIFWYSGLI*	-1.313	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25842	YCPPHLTHLFQINYT			15	SLAY-screened peptide P4192			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTGTCCTCCGCACTTGACTCACCTCTTCCAGATCAATTACACCTAGTTCAGCGATGTGTAA	YCPPHLTHLFQINYT*FSDV*	-1.313	0.010727	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25843	HLSSRRAWRVYVSPSSKYDH			20	SLAY-screened peptide P4193			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCTTTCCTCCCGGCGCGCGTGGCGTGTCTATGTTAGCCCCTCTTCGAAGTACGATCACTAA	HLSSRRAWRVYVSPSSKYDH*	-1.313	0.016461	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25844	YTSSCIIMDPAANSETAAVV			20	SLAY-screened peptide P4194			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATACTAGCTCGTGCATTATCATGGATCCGGCGGCGAACAGCGAGACGGCTGCCGTCGTTTAA	YTSSCIIMDPAANSETAAVV*	-1.313	0.000162	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25845	LPRHRGRCCINILHCRAKF			19	SLAY-screened peptide P4195			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCGCGTCACCGGGGCCGTTGCTGCATTAATATCCTTCACTGCCGCGCGAAGTTTTAACTG	LPRHRGRCCINILHCRAKF*L	-1.313	0.00016	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25846	FSASPDAGHLPGSSSNTLHD			20	SLAY-screened peptide P4196			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTCTGCTAGCCCGGACGCCGGCCACCTGCCTGGTTCGTCGAGTAATACCCTTCACGATTAA	FSASPDAGHLPGSSSNTLHD*	-1.313	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25847	VHGPPSRPAHVLMTLYYLSN			20	SLAY-screened peptide P4197			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCACGGGCCGCCTAGCCGGCCCGCGCATGTGCTCATGACCCTTTATTATCTCAGCAATTAA	VHGPPSRPAHVLMTLYYLSN*	-1.313	0.031918	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25848	LLHPCVDYRWMGTYYPLVIW			20	SLAY-screened peptide P4198			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCTCCATCCCTGCGTCGACTATAGGTGGATGGGTACTTACTATCCCTTGGTGATTTGGTAA	LLHPCVDYRWMGTYYPLVIW*	-1.313	0.003465	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25849	PSSLPCMCSINCPFYNFTIY			20	SLAY-screened peptide P4199			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCTTCTCTGCCGTGCATGTGCAGTATTAATTGCCCTTTTTATAATTTCACGATTTATTAA	PSSLPCMCSINCPFYNFTIY*	-1.313	0.033522	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25850	PNYHSESTGHSRLYRTHP			18	SLAY-screened peptide P4200			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAATTACCATAGTGAGAGCACCGGGCACAGTCGTCTTTACCGCACGCACCCCTAGTCGTAA	PNYHSESTGHSRLYRTHP*S*	-1.312	0.044487	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25851	LHQGSTAEGPSFRGKY			16	SLAY-screened peptide P4201			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCATCAGGGGAGTACTGCTGAGGGGCCTTCCTTTAGGGGTAAGTACTAGCAGTCCTCCTAA	LHQGSTAEGPSFRGKY*QSS*	-1.312	0.030941	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25852	GESQHQPDSPVLPHSPYLLC			20	SLAY-screened peptide P4202			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTGAGTCTCAGCATCAGCCTGACTCCCCCGTTCTGCCGCATTCCCCTTATTTGCTCTGTTAA	GESQHQPDSPVLPHSPYLLC*	-1.312	0.03431	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25853	CHSQPSDSPVYHALDIDYFL			20	SLAY-screened peptide P4203			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCATTCCCAGCCCAGTGACTCTCCCGTCTATCATGCGCTTGATATTGACTACTTCTTGTAA	CHSQPSDSPVYHALDIDYFL*	-1.312	0.011086	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25854	THSST			5	SLAY-screened peptide P4204			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCACTCTTCTACCTAGCACCTGGATAATAGTTGTTAGTTTTGCAAGCACACCGCTTTTTAA	THSST*HLDNSC*FCKHTAF*	-1.312	0.04353	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25855	PPPVIIRTNDANHCFATVLH			20	SLAY-screened peptide P4205			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCGCCTGTGATCATCCGCACTAATGATGCCAATCATTGCTTTGCTACGGTTCTTCATTAA	PPPVIIRTNDANHCFATVLH*	-1.312	5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25856	SALVSSRENLPIHSHYLNRP			20	SLAY-screened peptide P4206			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGCCCTGGTCTCTAGTCGTGAGAATTTGCCTATCCACTCCCATTACCTGAATCGTCCTTAA	SALVSSRENLPIHSHYLNRP*	-1.311	0.000441	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25857	PLADILCSDPYVSNHEYTIT			20	SLAY-screened peptide P4207			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTGGCCGATATCCTTTGTAGTGACCCCTACGTGAGTAATCACGAGTATACGATTACCTAA	PLADILCSDPYVSNHEYTIT*	-1.311	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25858	LVAHLGNS			8	SLAY-screened peptide P4208			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGTTGCCCACCTTGGTAACTCTTAGGATTCTTCGGCTAAGCCCAAGGCCTCGTTGATCTAA	LVAHLGNS*DSSAKPKASLI*	-1.311	0.00077	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25859	FPLMGFKYYYPCYTRSPSLY			20	SLAY-screened peptide P4209			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCCGTTGATGGGCTTTAAGTATTATTATCCTTGTTACACCCGTTCTCCGTCTCTTTATTAA	FPLMGFKYYYPCYTRSPSLY*	-1.311	0.001035	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25860	PVSPGRHVTIDCRTTVYRPP			20	SLAY-screened peptide P4210			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGTCAGCCCCGGGCGCCATGTTACGATTGACTGCAGGACGACCGTGTACCGCCCTCCGTAA	PVSPGRHVTIDCRTTVYRPP*	-1.311	0.03155	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25861	PHGWICTPSRLNYYLYSAVI			20	SLAY-screened peptide P4211			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCATGGCTGGATTTGCACCCCCAGTCGGTTGAACTATTACCTCTATTCTGCTGTCATCTAA	PHGWICTPSRLNYYLYSAVI*	-1.311	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25862	FIDERPLFHMA			11	SLAY-screened peptide P4212			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTATCGACGAGCGCCCCCTGTTCCATATGGCCTAGTATTTTTGCAACCCGCTTGCCAGGTAA	FIDERPLFHMA*YFCNPLAR*	-1.311	0.000321	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25863	NLSSTMARGTCSALRYLDVY			20	SLAY-screened peptide P4213			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCTTTCGAGTACTATGGCTCGCGGGACTTGTAGCGCTCTCCGTTATCTTGATGTTTACTAA	NLSSTMARGTCSALRYLDVY*	-1.31	0.00502	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25864	PCAFSSPLDALGFHGPYYVV			20	SLAY-screened peptide P4214			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGTGCCTTCTCTTCCCCGCTCGATGCTTTGGGTTTCCACGGTCCCTATTATGTTGTCTAA	PCAFSSPLDALGFHGPYYVV*	-1.31	0.002233	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25865	TTLDPLLPDFVPTTCDNSGKF			21	SLAY-screened peptide P4215			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACCCTGGATCCCTTGCTGCCCGACTTCGTTCCGACTACCTGCGATAATTCTGGCAAGTTT	TTLDPLLPDFVPTTCDNSGKF	-1.31	0.003326	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25866	PYGLPFCITYALAALTDRRM			20	SLAY-screened peptide P4216			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTACGGCCTCCCCTTTTGTATCACGTATGCGTTGGCGGCCCTCACCGACCGCCGTATGTAA	PYGLPFCITYALAALTDRRM*	-1.31	0.027752	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25867	WFDSYPYIPCRRCCRYLIRA			20	SLAY-screened peptide P4217			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTTTGACAGCTACCCTTATATTCCTTGTCGCCGCTGCTGTCGTTATCTCATTCGTGCCTAA	WFDSYPYIPCRRCCRYLIRA*	-1.31	0.00026	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25868	CLHSTNHVPKHWSAVPLSFN			20	SLAY-screened peptide P4218			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTGCATAGCACTAATCATGTGCCTAAGCACTGGAGCGCTGTGCCCCTTAGTTTCAATTAA	CLHSTNHVPKHWSAVPLSFN*	-1.31	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25869	STAKSHISIPMCSFYAIKSS			20	SLAY-screened peptide P4219			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTACCGCTAAGTCTCACATTTCTATCCCCATGTGTAGCTTCTACGCCATCAAGTCTTCTTAA	STAKSHISIPMCSFYAIKSS*	-1.31	0.001639	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25870	PILTTRRRALFYSARIPAIF			20	SLAY-screened peptide P4220			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATCTTGACCACTAGGCGCCGTGCCTTGTTCTACTCGGCCCGCATTCCCGCCATCTTTTAA	PILTTRRRALFYSARIPAIF*	-1.309	0.00022	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25871	LELYSSRAGSLLDAQASGCLT			21	SLAY-screened peptide P4221			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGGCTTGAACTTTACTCATCACGTGCAGGGTCACTTCTTGATGCCCAGGCCTCCGGATGCCTAACT	LELYSSRAGSLLDAQASGCLT	-1.309	0.00208	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25872	HGVRIMYNNSVPGIPSGAFFN			21	SLAY-screened peptide P4222			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGGCGTTCGCATTATGTATAATAACAGTGTACCTGGAATACCTTCGGGAGCTTTCTTTAAC	HGVRIMYNNSVPGIPSGAFFN	-1.309	0.008688	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25873	PSPYFSTATQYFCHYSFDYA			20	SLAY-screened peptide P4223			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGTCCCTACTTCAGTACCGCTACGCAGTATTTCTGCCACTACAGCTTTGACTATGCCTAA	PSPYFSTATQYFCHYSFDYA*	-1.309	5.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25874	LADRCKIGPFFNDMNHALSR			20	SLAY-screened peptide P4224			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGCCGACCGTTGCAAGATCGGCCCGTTTTTCAACGACATGAATCACGCGCTCTCCCGCTAA	LADRCKIGPFFNDMNHALSR*	-1.308	0.001963	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25875	VETFDSSDRVPFHTANT			17	SLAY-screened peptide P4225			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGAGACCTTCGACTCTTCTGACCGTGTTCCCTTCCACACCGCCAACACCTAGGCTATCTAA	VETFDSSDRVPFHTANT*AI*	-1.308	0.048067	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25876	LPNGLHHAVFEIWSTCIIYD			20	SLAY-screened peptide P4226			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCCAACGGCCTCCATCATGCCGTCTTCGAGATTTGGTCCACCTGCATCATTTACGACTAA	LPNGLHHAVFEIWSTCIIYD*	-1.308	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25877	VPNHHPVVVCE			11	SLAY-screened peptide P4227			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCCCAATCACCACCCCGTTGTGGTTTGTGAGTAGAATTCTCCTCTCGCGTGTGATAATTAA	VPNHHPVVVCE*NSPLACDN*	-1.308	0.000145	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25878	TRRARRTHRVHAAVLNSPTP			20	SLAY-screened peptide P4228			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCGCCGCGCCCGTCGCACTCATCGGGTTCATGCCGCGGTCCTCAACTCGCCCACCCCTTAA	TRRARRTHRVHAAVLNSPTP*	-1.308	0.000457	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25879	GTTFICNIPHSFNLHRSC			18	SLAY-screened peptide P4229			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCACTACCTTTATCTGTAACATTCCTCATTCTTTCAATTTGCATCGGTCTTGTTAGCAGTAA	GTTFICNIPHSFNLHRSC*Q*	-1.307	7.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25880	SCA			3	SLAY-screened peptide P4230			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTGCGCATAATAACGCGCCTCTGAAGTACCGCGACGCCAATTTTCCGTCTGCCCTTACCTA	SCA**RASEVPRRQFSVCPYL	-1.307	0.000985	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25881	CHTAYNLFRPRPSSGAARLE			20	SLAY-screened peptide P4231			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCATACCGCTTACAATCTGTTCCGCCCCCGTCCGTCCTCCGGCGCTGCTCGGTTGGAGTAA	CHTAYNLFRPRPSSGAARLE*	-1.307	3.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25882	PRSDIATMQAPESISFPPGY			20	SLAY-screened peptide P4232			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGCTCCGACATCGCTACTATGCAGGCCCCCGAGAGTATCAGTTTCCCCCCGGGCTATTAA	PRSDIATMQAPESISFPPGY*	-1.307	0.022858	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25883	LSRPINFPRP			10	SLAY-screened peptide P4233			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGAGTCGTCCCATTAACTTTCCGCGCCCCTAGGTTCAGAATAAGTCCTACACTTTTAGTTAA	LSRPINFPRP*VQNKSYTFS*	-1.307	3.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25884	CCTTWWNHCLLSHPFKNTSY			20	SLAY-screened peptide P4234			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTGTACTACTTGGTGGAACCATTGCCTGCTTAGTCACCCCTTTAAGAATACCAGCTACTAA	CCTTWWNHCLLSHPFKNTSY*	-1.307	0.043469	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25885	TVRHTISLLRQRLNRFLYRV			20	SLAY-screened peptide P4235			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTCCGGCACACTATTTCGTTGCTCCGTCAGCGGCTTAACCGTTTTTTGTACCGCGTTTAA	TVRHTISLLRQRLNRFLYRV*	-1.307	0.04353	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25886	RAG			3	SLAY-screened peptide P4236			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGCCGGGTAGCTTACTATTACCTAGGCTACTAGCACGAGGCATAGTTCTAATATTGGTTAA	RAG*LTIT*ATSTRHSSNIG*	-1.307	0.007861	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25887	PDFRLVCYHPERL			13	SLAY-screened peptide P4237			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGACTTCCGTCTTGTTTGTTACCACCCCGAGCGTCTCTAGGCCAGGAATCTGAGCAAGTAA	PDFRLVCYHPERL*ARNLSK*	-1.307	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25888	CDSPYYDIPLRWSIL			15	SLAY-screened peptide P4238			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGACTCGCCGTACTACGATATTCCGCTCCGTTGGTCGATTCTTTAGTTTCGGAGCGACTAA	CDSPYYDIPLRWSIL*FRSD*	-1.307	0.001914	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25889	CVTL			4	SLAY-screened peptide P4239			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGTTACCTTGTAGCGTTCCATCACGTAGCCCGACTTCGTTTACATTTATACTATTAGTTAA	CVTL*RSIT*PDFVYIYTIS*	-1.307	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25890	RGKHARAYRSWPWIYVLFAM			20	SLAY-screened peptide P4240			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGGCAAGCATGCCCGGGCTTACCGGAGTTGGCCCTGGATTTATGTTCTGTTCGCTATGTAA	RGKHARAYRSWPWIYVLFAM*	-1.307	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25891	PIHFLAGPGYL			11	SLAY-screened peptide P4241			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATTCACTTTTTGGCCGGTCCTGGCTACCTCTAGTTTACCTTTGCTTATAAGAATTGCTAA	PIHFLAGPGYL*FTFAYKNC*	-1.306	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25892	LCTAFSVYLAQSLKALRHILT			21	SLAY-screened peptide P4242			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTGCACTGCTTTTTCCGTGTACCTGGCTCAGAGTCTCAAGGCTCTGCGCCATATATTAACT	LCTAFSVYLAQSLKALRHILT	-1.306	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25893	LFVPLHSHTPVWEPIYANSV			20	SLAY-screened peptide P4243			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTTGTGCCTCTCCATTCCCACACTCCGGTCTGGGAGCCCATCTACGCCAACTCTGTTTAA	LFVPLHSHTPVWEPIYANSV*	-1.306	0.00967	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25894	HHHCNAYHLRYYHGIPVLRS			20	SLAY-screened peptide P4244			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCATCATTGCAACGCTTATCACCTCCGGTACTACCACGGGATCCCCGTCCTGCGTTCGTAA	HHHCNAYHLRYYHGIPVLRS*	-1.306	0.011325	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25895	SQCTNYPDYFLLSYASLGTT			20	SLAY-screened peptide P4245			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCAGTGTACTAACTATCCTGATTACTTTCTTCTGTCTTATGCTTCCCTTGGGACTACGTAA	SQCTNYPDYFLLSYASLGTT*	-1.306	4.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25896	QTQPPHDSEEADKCLYPVII			20	SLAY-screened peptide P4246			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGACCCAGCCGCCTCATGACAGCGAGGAGGCGGATAAGTGCTTGTATCCCGTGATTATTTAA	QTQPPHDSEEADKCLYPVII*	-1.306	1.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25897	HSQRCGMD			8	SLAY-screened peptide P4247			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAGCCAGCGCTGTGGCATGGATTAGATTCAGATTAATTACTCCGACATCGGTATTTCGTAA	HSQRCGMD*IQINYSDIGIS*	-1.306	0.000846	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25898	ETRLHNPRISHNNYWWFMLF			20	SLAY-screened peptide P4248			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGACTCGGCTGCATAATCCTCGCATTAGCCACAATAACTATTGGTGGTTCATGCTTTTTTAA	ETRLHNPRISHNNYWWFMLF*	-1.306	4.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25899	TLSTWDLDHWQCHPRFTTTY			20	SLAY-screened peptide P4249			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTGAGCACTTGGGACCTTGACCACTGGCAGTGCCATCCCAGGTTTACTACCACCTATTAA	TLSTWDLDHWQCHPRFTTTY*	-1.305	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25900	LPSP			4	SLAY-screened peptide P4250			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCGTCGCCCTAGCCGGCCCCCGGCGAGCGCCGCAGTGACGTTCGTCATAACCCCTACTAA	LPSP*PAPGERRSDVRHNPY*	-1.305	0.000504	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25901	YCFSLMVVIRSSRLTLRFRCN			21	SLAY-screened peptide P4251			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTGTTTCAGCTTGATGGTGGTCATTAGGTCATCACGTCTTACTCTGAGGTTCCGATGTAAC	YCFSLMVVIRSSRLTLRFRCN	-1.305	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25902	PSTHHTQNCYEGLVIDYHER			20	SLAY-screened peptide P4252			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGCACGCATCACACGCAGAACTGCTATGAGGGCCTTGTTATCGACTACCACGAGCGCTAA	PSTHHTQNCYEGLVIDYHER*	-1.305	0.040617	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25903	DHRIVTLLTLCLVLILLTIRN			21	SLAY-screened peptide P4253			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCATCGTATTGTAACGCTTTTGACTCTATGCCTAGTATTAATTCTTCTAACTATACGTAAC	DHRIVTLLTLCLVLILLTIRN	-1.305	1.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25904	ILNRNPYNAPMNLNDN			16	SLAY-screened peptide P4254			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCTTAACCGCAACCCCTATAACGCGCCCATGAACCTTAACGATAACTAGTGGGAGACGTAA	ILNRNPYNAPMNLNDN*WET*	-1.304	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25905	LRLLPCPIPNPYVYLAFLGN			20	SLAY-screened peptide P4255			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCGGTTGCTCCCCTGCCCTATTCCCAACCCCTACGTTTATCTTGCGTTTCTCGGCAACTAA	LRLLPCPIPNPYVYLAFLGN*	-1.304	0.000578	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25906	CNMLLPYLLNGQIYLSYNYH			20	SLAY-screened peptide P4256			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAATATGCTGCTGCCGTACCTGCTTAATGGCCAGATCTATCTTAGCTATAACTACCACTAA	CNMLLPYLLNGQIYLSYNYH*	-1.304	0.006412	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25907	TPFNYDDLRTIVNAISPFSP			20	SLAY-screened peptide P4257			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCCGTTTAACTATGACGATCTCCGCACGATCGTGAATGCCATTTCCCCCTTTAGCCCCTAA	TPFNYDDLRTIVNAISPFSP*	-1.303	0.025142	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25908	NANDPVVPHIRLPTISYILL			20	SLAY-screened peptide P4258			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGCCAATGATCCGGTGGTCCCTCATATCAGGCTGCCTACTATTTCGTATATCCTTCTTTAA	NANDPVVPHIRLPTISYILL*	-1.303	0.000325	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25909	DDFLSTFYMLAIATLLHMRP			20	SLAY-screened peptide P4259			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGATTTCCTTAGCACGTTTTATATGCTCGCTATCGCTACTCTTCTTCATATGCGCCCTTAA	DDFLSTFYMLAIATLLHMRP*	-1.303	0.000714	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25910	IYPFRPFAYTPPRLQPGPSS			20	SLAY-screened peptide P4260			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTTACCCGTTCCGCCCTTTCGCCTATACGCCTCCCCGCCTCCAGCCGGGGCCCTCCTCTTAA	IYPFRPFAYTPPRLQPGPSS*	-1.303	0.004067	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25911	AHIGDAMLSFRHHKTSPVHG			20	SLAY-screened peptide P4261			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCACATCGGGGACGCCATGCTTTCCTTTCGCCATCATAAGACTAGTCCCGTCCACGGGTAA	AHIGDAMLSFRHHKTSPVHG*	-1.303	0.038908	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25912	RLKHRSSSQAWYSQIVAFIR			20	SLAY-screened peptide P4262			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCTTAAGCACCGGTCTAGTAGTCAGGCGTGGTACTCCCAGATTGTCGCGTTTATTCGCTAA	RLKHRSSSQAWYSQIVAFIR*	-1.303	0.016741	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25913	RAYYI			5	SLAY-screened peptide P4263			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCTTATTACATCTAGGCCTACTGTTTGCACATTCCGAGGAACGGCCTGATGGGCTAACTG	RAYYI*AYCLHIPRNGLMG*L	-1.303	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25914	APGISCMLRGTPVVYYSRNA			20	SLAY-screened peptide P4264			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCCGGGATTTCGTGTATGCTTCGCGGGACCCCGGTTGTTTATTACAGTAGGAATGCGTAA	APGISCMLRGTPVVYYSRNA*	-1.303	2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25915	PHLYSLYHKSLCSGISLYYF			20	SLAY-screened peptide P4265			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCACCTGTATTCGCTCTATCACAAGAGTCTTTGTTCTGGTATTTCCCTTTATTATTTCTAA	PHLYSLYHKSLCSGISLYYF*	-1.303	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25916	YLPSFKRSSIHCVPYDEHH			19	SLAY-screened peptide P4266			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCTTCCTTCGTTCAAGAGGAGCTCCATCCATTGTGTGCCGTACGATGAGCACCACTAACTG	YLPSFKRSSIHCVPYDEHH*L	-1.303	0.0002	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25917	VSRLSLAIRPACQSCAQHSR			20	SLAY-screened peptide P4267			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTCCAGGCTGTCCCTCGCTATTCGTCCCGCGTGCCAGTCCTGTGCCCAGCATAGCCGTTAA	VSRLSLAIRPACQSCAQHSR*	-1.303	0.00708	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25918	NTLVTSGHVTRAHALFLRVC			20	SLAY-screened peptide P4268			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATACCCTTGTCACCTCGGGCCATGTTACTCGTGCTCATGCCCTCTTCCTTCGCGTTTGCTAA	NTLVTSGHVTRAHALFLRVC*	-1.303	0.044007	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25919	PPPCVLTTHA			10	SLAY-screened peptide P4269			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCTCCCTGTGTCCTGACGACCCACGCCTAGGATACCCCTCTGGCGCCTCGGCGTATCTAA	PPPCVLTTHA*DTPLAPRRI*	-1.302	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25920	LNATTNNPINDIDCDTTSMP			20	SLAY-screened peptide P4270			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAACGCCACCACCAACAACCCCATCAATGACATCGATTGTGATACGACGAGTATGCCCTAA	LNATTNNPINDIDCDTTSMP*	-1.302	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25921	PRDAHLFYPYGFYSYL			16	SLAY-screened peptide P4271			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGGGATGCTCATCTTTTCTATCCTTATGGTTTCTATTCCTACTTGTAGGTCGACTATTAA	PRDAHLFYPYGFYSYL*VDY*	-1.302	0.006183	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25922	SLSR			4	SLAY-screened peptide P4272			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCTCTCCCGCTAGTCGGGCAGCGGGCGCTACGTCATGCCGTGTAGCGTTTATACTTATTAA	SLSR*SGSGRYVMPCSVYTY*	-1.302	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25923	SLSTYGIHVPGYCLPINTMW			20	SLAY-screened peptide P4273			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCTTTCCACGTATGGCATCCATGTTCCTGGCTATTGCCTCCCCATCAACACTATGTGGTAA	SLSTYGIHVPGYCLPINTMW*	-1.301	0.000775	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25924	HAYTARVTPRTAETYHPHAS			20	SLAY-screened peptide P4274			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCCTATACCGCTCGCGTGACCCCGCGTACTGCGGAGACTTATCATCCGCACGCCAGCTAA	HAYTARVTPRTAETYHPHAS*	-1.301	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25925	LDKFYLPQAFSRIESTPICH			20	SLAY-screened peptide P4275			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGACAAGTTTTATTTGCCCCAGGCTTTCTCGCGCATTGAGTCTACGCCTATTTGTCACTAA	LDKFYLPQAFSRIESTPICH*	-1.301	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25926	PSQIVSYIYSPWADWHTLRQ			20	SLAY-screened peptide P4276			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCGCAGATCGTTTCTTACATCTATTCTCCCTGGGCGGATTGGCATACCCTTCGCCAGTAA	PSQIVSYIYSPWADWHTLRQ*	-1.301	0.000608	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25927	PFSYPMYYTTLYNII			15	SLAY-screened peptide P4277			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTTAGTTACCCGATGTATTATACCACCCTTTATAACATTATTTAGGCCGACGGTAACTAA	PFSYPMYYTTLYNII*ADGN*	-1.301	0.000119	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25928	PTYTRQYDRTHEPVYYFYLL			20	SLAY-screened peptide P4278			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACTTACACCAGGCAGTACGATCGTACGCATGAGCCGGTTTATTATTTCTACCTGCTCTAA	PTYTRQYDRTHEPVYYFYLL*	-1.301	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25929	RMLYFYWADDSFKIRPCAII			20	SLAY-screened peptide P4279			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTATGTTGTACTTTTACTGGGCGGATGACTCCTTTAAGATCCGCCCGTGCGCTATCATTTAA	RMLYFYWADDSFKIRPCAII*	-1.301	0.005614	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25930	SWSSTWIKFPVRFSGPTCRV			20	SLAY-screened peptide P4280			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTGGTCGTCCACGTGGATCAAGTTTCCGGTTCGCTTTTCCGGGCCCACGTGCCGCGTCTAA	SWSSTWIKFPVRFSGPTCRV*	-1.301	0.032659	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25931	WFARGIPCTFV			11	SLAY-screened peptide P4281			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTTCGCCCGGGGCATTCCGTGTACTTTCGTGTAGGTTGCCGACTTCACGGGCAACTCTTAA	WFARGIPCTFV*VADFTGNS*	-1.3	0.035738	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25932	ARCTIHRILPAGHKIN			16	SLAY-screened peptide P4282			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCGGTGCACCATCCACCGTATTTTGCCTGCCGGTCATAAGATTAATTAGGCCTAGAAGAAC	ARCTIHRILPAGHKIN*A*KN	-1.3	0.007877	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25933	VCWQCHTVCPKRTDNWPWLV			20	SLAY-screened peptide P4283			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTGTTGGCAGTGCCATACCGTGTGCCCGAAGCGTACCGATAACTGGCCTTGGCTTGTTTAA	VCWQCHTVCPKRTDNWPWLV*	-1.3	0.000831	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25934	AYHMAVLRQPSNDPYKHNAT			20	SLAY-screened peptide P4284			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTATCACATGGCCGTGCTCAGGCAGCCTTCTAATGACCCTTATAAGCACAACGCTACTTAA	AYHMAVLRQPSNDPYKHNAT*	-1.3	0.030205	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25935	NTPTDAAHNRMYSCVPISST			20	SLAY-screened peptide P4285			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATACCCCCACTGACGCCGCTCATAACCGCATGTACTCGTGCGTGCCTATTTCTAGCACCTAA	NTPTDAAHNRMYSCVPISST*	-1.3	0.033596	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25936	YSLSRHIVCSSTRRFDSFST			20	SLAY-screened peptide P4286			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTCCTTGTCTCGCCACATCGTCTGTTCGAGCACTCGCAGGTTTGATTCCTTCAGCACTTAA	YSLSRHIVCSSTRRFDSFST*	-1.299	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25937	DTTWR			5	SLAY-screened peptide P4287			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACACTACTTGGAGGTAGGTGTCCAAGCGCGCCCAGCCCCATTAGCTTGGTACCCCTTTCTAA	DTTWR*VSKRAQPH*LGTPF*	-1.299	0.000292	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25938	TGRRLAKLTVLPLTSRQSRPL			21	SLAY-screened peptide P4288			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGGGCGCCGGCTTGCTAAGCTTACCGTCCTTCCGCTCACTAGTCGGCAGTCACGACCTCTA	TGRRLAKLTVLPLTSRQSRPL	-1.299	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25939	TALGLPPTVSFRLSKFSHAC			20	SLAY-screened peptide P4289			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGCCCTGGGCTTGCCCCCGACTGTCTCGTTCCGCCTTAGCAAGTTTAGCCACGCTTGTTAA	TALGLPPTVSFRLSKFSHAC*	-1.299	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25940	HLSLRAVLLQRIPQFNAFIL			20	SLAY-screened peptide P4290			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCTTAGCCTCAGGGCCGTCCTTCTGCAGCGTATCCCTCAGTTTAACGCTTTTATTCTTTAA	HLSLRAVLLQRIPQFNAFIL*	-1.299	0.043255	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25941	PSYAPYSWTCFGPTYLNALI			20	SLAY-screened peptide P4291			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCCTATGCCCCTTATTCTTGGACCTGCTTTGGGCCGACTTACCTTAATGCTCTTATTTAA	PSYAPYSWTCFGPTYLNALI*	-1.299	0.047997	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25942	AHFCIDCTFHHDYK			14	SLAY-screened peptide P4292			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCATTTCTGCATTGATTGCACGTTCCACCACGATTATAAGTAGCTGCTGCGACCAGCTAAC	AHFCIDCTFHHDYK*LLRPAN	-1.298	0.00049	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25943	RKTGLRARSVPNDRHALATV			20	SLAY-screened peptide P4293			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGAAGACTGGGCTGCGCGCGCGTTCCGTCCCGAACGATCGCCATGCGCTCGCCACCGTTTAA	RKTGLRARSVPNDRHALATV*	-1.298	0.001717	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25944	RGPATLWGHFQNPISLLSVN			20	SLAY-screened peptide P4294			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGGGTCCCGCGACGCTCTGGGGGCACTTTCAGAACCCTATTTCTCTGTTGTCCGTGAATTAA	RGPATLWGHFQNPISLLSVN*	-1.298	4.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25945	LVNSRAVLSRTLPIQIRKPP			20	SLAY-screened peptide P4295			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGTCAATTCGCGCGCTGTTCTCTCCAGGACTTTGCCCATTCAGATTCGGAAGCCCCCGTAA	LVNSRAVLSRTLPIQIRKPP*	-1.298	3.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25946	SRWSIMSRSHTDRTHPPIYP			20	SLAY-screened peptide P4296			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCGCTGGTCGATTATGTCGCGCTCTCATACTGATCGCACTCACCCCCCTATTTATCCGTAA	SRWSIMSRSHTDRTHPPIYP*	-1.298	0.000399	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25947	RCESTYWRTGHYTSTHTIHK			20	SLAY-screened peptide P4297			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGTGAGAGCACTTATTGGCGTACCGGGCACTACACGTCGACTCATACTATCCATAAGTAA	RCESTYWRTGHYTSTHTIHK*	-1.297	0.002976	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25948	ANYTDFFICFLGAFRIL			17	SLAY-screened peptide P4298			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAATTACACCGACTTCTTCATCTGCTTTCTAGGCGCATTTCGAATACTCTGACTTTCTAAC	ANYTDFFICFLGAFRIL*LSN	-1.297	0.017517	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25949	SYHNNCDVRAYELPVPTWHF			20	SLAY-screened peptide P4299			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTACCACAACAACTGTGATGTTCGTGCTTATGAGCTGCCTGTTCCTACCTGGCATTTTTAA	SYHNNCDVRAYELPVPTWHF*	-1.297	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25950	HSLRSDPEPRPRTTPCICPN			20	SLAY-screened peptide P4300			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTCCTTGCGTAGTGATCCTGAGCCGCGTCCTCGCACCACGCCGTGCATCTGCCCCAATTAA	HSLRSDPEPRPRTTPCICPN*	-1.297	0.001493	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25951	PKNTARYDRTSYKVYITRLK			20	SLAY-screened peptide P4301			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAAGAATACCGCCCGCTATGACAGGACGTCTTATAAGGTTTATATCACCCGGCTGAAGTAA	PKNTARYDRTSYKVYITRLK*	-1.297	0.003155	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25952	GCQSLHDSCIYFPSHYNVCMY			21	SLAY-screened peptide P4302			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGTGCCAGTCCCTCCATGATTCTTGCATCTACTTTCCTAGTCATTACAATGTGTGCATGTAC	GCQSLHDSCIYFPSHYNVCMY	-1.296	0.000518	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25953	VPLCAWSPPTLCLRSDNVPQ			20	SLAY-screened peptide P4303			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCCCCTCTGCGCCTGGAGTCCTCCCACCCTCTGTCTGAGGTCCGACAACGTTCCCCAGTAA	VPLCAWSPPTLCLRSDNVPQ*	-1.296	0.002315	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25954	VSHKCIGSPSADWNRPSAGI			20	SLAY-screened peptide P4304			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCAGTCACAAGTGCATTGGTAGTCCTTCCGCGGATTGGAACCGTCCGTCGGCGGGCATTTAA	VSHKCIGSPSADWNRPSAGI*	-1.296	0.00049	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25955	PPDLPGNTIPFQCMPQKAH			19	SLAY-screened peptide P4305			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCGGACCTTCCGGGCAATACCATCCCGTTCCAGTGCATGCCGCAGAAGGCGCATTAGTAA	PPDLPGNTIPFQCMPQKAH**	-1.296	0.000245	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25956	YCPPIPAHAFCLHPTMWCHI			20	SLAY-screened peptide P4306			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTGCCCCCCTATTCCGGCGCACGCCTTTTGTCTTCACCCTACGATGTGGTGCCATATTTAA	YCPPIPAHAFCLHPTMWCHI*	-1.296	0.01233	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25957	AVVDCPWHHFNPLRSRLHSP			20	SLAY-screened peptide P4307			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGTGGTCGACTGTCCCTGGCACCACTTTAATCCGCTCAGGTCGAGGCTCCACTCCCCCTAA	AVVDCPWHHFNPLRSRLHSP*	-1.296	0.000873	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25958	YHLNIPYSVRKCCFMFFTRC			20	SLAY-screened peptide P4308			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCACTTGAACATCCCCTACAGTGTGAGGAAGTGCTGCTTCATGTTCTTCACTCGCTGCTAA	YHLNIPYSVRKCCFMFFTRC*	-1.296	0.001338	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25959	QVLLSTLRGVYLYTKHYLNP			20	SLAY-screened peptide P4309			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGTTCTTCTTTCCACCCTGAGGGGCGTCTATCTGTATACTAAGCATTACCTCAATCCTTAA	QVLLSTLRGVYLYTKHYLNP*	-1.296	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25960	THLSSESLLLGLSNTLHFYR			20	SLAY-screened peptide P4310			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCACCTTTCCAGCGAGTCGCTCCTCCTTGGCCTTTCTAACACCCTTCATTTTTACAGGTAA	THLSSESLLLGLSNTLHFYR*	-1.295	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25961	NSLTVPHLIRYVETIIGSRY			20	SLAY-screened peptide P4311			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTCGTTGACGGTCCCCCACCTTATCAGGTATGTTGAGACGATCATCGGTTCTCGTTACTAA	NSLTVPHLIRYVETIIGSRY*	-1.295	0.014383	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25962	TSCPFNLVHGLGP			13	SLAY-screened peptide P4312			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAGCTGCCCGTTCAACCTCGTCCATGGTCTGGGGCCGTAGAAGCCCCGCACCGAGCCTTAA	TSCPFNLVHGLGP*KPRTEP*	-1.295	0.000768	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25963	SLDSMAPAVCVKPNYIPLII			20	SLAY-screened peptide P4313			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCTGGACTCGATGGCTCCGGCCGTGTGCGTTAAGCCGAACTATATCCCGCTTATTATCTAA	SLDSMAPAVCVKPNYIPLII*	-1.295	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25964	LLWALVSPIPWLVHSDGKFQ			20	SLAY-screened peptide P4314			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCTCTGGGCCTTGGTCAGTCCTATCCCTTGGCTCGTCCATTCCGATGGTAAGTTTCAGTAA	LLWALVSPIPWLVHSDGKFQ*	-1.295	0.04805	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25965	PLPINPNSPAITPAPQITFH			20	SLAY-screened peptide P4315			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTTGCCCATTAACCCGAACTCTCCGGCTATTACCCCGGCTCCCCAGATTACGTTTCACTAA	PLPINPNSPAITPAPQITFH*	-1.294	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25966	TFTCRPVTLTNDS			13	SLAY-screened peptide P4316			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTTTACCTGCAGGCCCGTGACGCTGACGAACGACTCGTAGCCGGACTCTAGTCAGCGCTAA	TFTCRPVTLTNDS*PDSSQR*	-1.294	0.004516	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25967	ILRLQPCLLCLFIVS			15	SLAY-screened peptide P4317			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCTTCGGCTGCAGCCTTGTCTCCTTTGTCTCTTTATCGTGTCTTAGAAGGGTGGTATGTAA	ILRLQPCLLCLFIVS*KGGM*	-1.294	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25968	SHQAYIAHQVHNAHIRGPPH			20	SLAY-screened peptide P4318			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCATCAGGCCTACATTGCCCATCAGGTTCACAATGCTCACATCCGTGGGCCGCCCCACTAA	SHQAYIAHQVHNAHIRGPPH*	-1.294	0.000882	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25969	PHLCMTCLGLPNDPRSIWTH			20	SLAY-screened peptide P4319			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCATTTGTGCATGACCTGTCTTGGGTTGCCGAATGATCCCCGCTCCATTTGGACGCATTAA	PHLCMTCLGLPNDPRSIWTH*	-1.294	0.011867	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25970	PHHWFSARLDSSHATQGAHR			20	SLAY-screened peptide P4320			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCACCACTGGTTTTCCGCCCGCCTTGATTCTTCCCATGCTACTCAGGGTGCCCATCGCTAA	PHHWFSARLDSSHATQGAHR*	-1.294	0.046612	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25971	RCSPTLDSHYNMCNCVRNYS			20	SLAY-screened peptide P4321			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTGCTCCCCGACCCTGGACTCTCATTACAACATGTGCAACTGTGTTCGTAATTACAGCTAA	RCSPTLDSHYNMCNCVRNYS*	-1.293	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25972	LLHFYPSLWLGRTRRPIRPCN			21	SLAY-screened peptide P4322			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCTCCATTTTTACCCGTCGCTGTGGCTCGGACGAACTCGCCGCCCAATCCGTCCGTGTAAC	LLHFYPSLWLGRTRRPIRPCN	-1.293	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25973	PTLPLFDKTRGYRRTTSVFK			20	SLAY-screened peptide P4323			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACCCTGCCGCTCTTTGACAAGACTCGGGGCTATCGGAGGACTACTAGCGTTTTTAAGTAA	PTLPLFDKTRGYRRTTSVFK*	-1.293	0.001805	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25974	PIVMGRARCTIWNAIIDLRS			20	SLAY-screened peptide P4324			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATCGTCATGGGTCGCGCCCGGTGTACCATCTGGAATGCCATTATTGATCTCCGCAGTTAA	PIVMGRARCTIWNAIIDLRS*	-1.293	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25975	TALSVENTYSTWLCAPNSLL			20	SLAY-screened peptide P4325			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGCTCTGTCCGTGGAGAATACGTATAGCACTTGGTTGTGCGCCCCCAACTCCCTGCTCTAA	TALSVENTYSTWLCAPNSLL*	-1.293	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25976	PRMK			4	SLAY-screened peptide P4326			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGCATGAAGTAGTCTCTGAGCACCTCGACTGAGCGCGCCTGTAACAACCATCCCAAGTAA	PRMK*SLSTSTERACNNHPK*	-1.293	0.001285	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25977	IRSVAPNACFKQENLDAFTS			20	SLAY-screened peptide P4327			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCGCTCCGTCGCCCCCAACGCGTGCTTCAAGCAGGAGAATCTCGACGCTTTCACCTCCTAA	IRSVAPNACFKQENLDAFTS*	-1.293	0.024716	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25978	FQSNYNGARHVFVN			14	SLAY-screened peptide P4328			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCAGAGTAACTACAACGGTGCGAGGCACGTTTTCGTGAACTAGTGTTGTCACATTTACTAA	FQSNYNGARHVFVN*CCHIY*	-1.293	0.017738	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25979	GTSRSN			6	SLAY-screened peptide P4329			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCACTTCTCGGTCCAATTAGGCTCACCCGGGGATTACTATGGACCCGGAGCTCTTTACCTAA	GTSRSN*AHPGITMDPELFT*	-1.293	0.033007	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25980	DDSTTGCISTHSAVYSMSLL			20	SLAY-screened peptide P4330			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGACAGCACCACCGGTTGTATTTCTACCCACTCGGCTGTGTATTCTATGTCCCTGCTTTAA	DDSTTGCISTHSAVYSMSLL*	-1.292	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25981	ACGIPLIRARSICLFCLN			18	SLAY-screened peptide P4331			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTGCGGGATCCCACTTATACGGGCCCGATCTATTTGCTTATTTTGCCTTAACTGAGTAAGT	ACGIPLIRARSICLFCLN*VS	-1.292	0.001576	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25982	ERICFLGAENHTTSLYTHVD			20	SLAY-screened peptide P4332			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCGTATTTGCTTCCTTGGGGCCGAGAATCACACTACTTCCTTGTATACTCACGTTGATTAA	ERICFLGAENHTTSLYTHVD*	-1.291	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25983	AVDTSSPRT			9	SLAY-screened peptide P4333			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGTTGACACCTCCTCGCCTCGCACCTAGTATTCTCATTCTATCGACCATATTATTGCTTAA	AVDTSSPRT*YSHSIDHIIA*	-1.291	0.040637	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25984	TILLSRLLGRATLLAMIIPCN			21	SLAY-screened peptide P4334			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTATCTTGCTTAGCCGGCTGCTTGGCCGTGCCACACTTCTGGCGATGATAATACCGTGTAAC	TILLSRLLGRATLLAMIIPCN	-1.291	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25985	ILKNYLFTDF			10	SLAY-screened peptide P4335			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCTTAAGAACTATCTTTTCACCGATTTTTAGATGCTCAGGTTGCTCGCTATTTGTAGGTAA	ILKNYLFTDF*MLRLLAICR*	-1.291	0.001478	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25986	LCAMVSSFAYVRMVADDLFT			20	SLAY-screened peptide P4336			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTGTGCTATGGTGTCTAGTTTCGCCTACGTGCGTATGGTTGCTGACGATCTTTTTACCTAA	LCAMVSSFAYVRMVADDLFT*	-1.291	0.009915	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25987	DSSAYAPIYMLSVYRVSKYE			20	SLAY-screened peptide P4337			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACAGTTCGGCCTACGCTCCGATTTACATGCTTAGCGTTTACCGTGTCAGCAAGTATGAGTAA	DSSAYAPIYMLSVYRVSKYE*	-1.29	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25988	NKAERGLTVNHVR			13	SLAY-screened peptide P4338			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATAAGGCTGAGCGTGGGTTGACCGTTAATCACGTTAGGTAGGACGTTACGAATTACCTGTAA	NKAERGLTVNHVR*DVTNYL*	-1.29	0.019204	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25989	RSLLGAFRRCPAVSIWSTLFN			21	SLAY-screened peptide P4339			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGAAGTCTCCTAGGCGCTTTTAGACGGTGCCCCGCTGTGTCTATATGGAGTACGTTGTTTAAC	RSLLGAFRRCPAVSIWSTLFN	-1.289	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25990	CNHFSAKLMRNTHGPPDIIL			20	SLAY-screened peptide P4340			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAATCATTTCAGCGCCAAGCTGATGCGGAATACCCACGGCCCTCCGGATATCATTCTGTAA	CNHFSAKLMRNTHGPPDIIL*	-1.289	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25991	IACRTIPATKMPCCLCMDKTY			21	SLAY-screened peptide P4341			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGCCTGCCGGACCATTCCGGCCACCAAGATGCCTTGCTGTTTGTGTATGGATAAGACGTAC	IACRTIPATKMPCCLCMDKTY	-1.289	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25992	TDFATTCFTPDTLFISIWCV			20	SLAY-screened peptide P4342			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGACTTTGCGACCACGTGCTTTACTCCCGACACTCTCTTCATTAGCATCTGGTGCGTTTAA	TDFATTCFTPDTLFISIWCV*	-1.289	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25993	ATSDCV			6	SLAY-screened peptide P4343			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTACGTCCGATTGTGTGTAGTGGATCAGCCGCTCTCCCACTTTTTCCATGGACTGGACCTAA	ATSDCV*WISRSPTFSMDWT*	-1.289	6.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25994	CNRGRRSGTYSIRESHRCPL			20	SLAY-screened peptide P4344			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAACAGGGGGAGGAGGAGCGGGACCTATAGTATTCGTGAGTCTCACCGGTGTCCGTTGTAA	CNRGRRSGTYSIRESHRCPL*	-1.289	0.048548	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25995	QWDAISSLFGKTFS			14	SLAY-screened peptide P4345			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTGGGATGCTATTAGCTCTCTCTTTGGCAAGACGTTCAGTTAGCTTTATATCCATATCTAA	QWDAISSLFGKTFS*LYIHI*	-1.289	0.012027	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25996	RATLIVRRLLSLLRFVRGICN			21	SLAY-screened peptide P4346			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCCACTCTAATAGTAAGACGTCTCCTTTCATTGCTCCGCTTCGTCCGGGGCATTTGTAAC	RATLIVRRLLSLLRFVRGICN	-1.289	0.043729	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25997	HTETSQVDDTTTPLYVSCFI			20	SLAY-screened peptide P4347			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACTGAGACCTCTCAGGTGGATGATACGACGACTCCTCTCTACGTTTCTTGCTTTATCTAA	HTETSQVDDTTTPLYVSCFI*	-1.289	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25998	TPRVSVSPLAARIIAPSRSN			20	SLAY-screened peptide P4348			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCTCGCGTCTCAGTAAGTCCGCTGGCCGCACGAATAATAGCCCCGAGCCGCTCTAACTGA	TPRVSVSPLAARIIAPSRSN*	-1.289	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP25999	SLAHHFIRPPMLLTTFSARV			20	SLAY-screened peptide P4349			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCTTGCCCACCATTTTATCCGTCCGCCCATGCTTCTCACGACTTTCAGCGCGCGTGTCTAA	SLAHHFIRPPMLLTTFSARV*	-1.288	0.003911	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26000	SEQSVKCPSPYYLSM			15	SLAY-screened peptide P4350			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGAGCAGAGCGTTAAGTGCCCGAGCCCCTACTACCTTTCCATGTAGAACGCGGATGATTAA	SEQSVKCPSPYYLSM*NADD*	-1.288	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26001	HTDICSPYRPPLHTVKIEST			20	SLAY-screened peptide P4351			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACTGATATTTGCAGTCCCTATCGTCCCCCGTTGCATACTGTGAAGATTGAGAGCACGTAA	HTDICSPYRPPLHTVKIEST*	-1.288	0.000206	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26002	SGLTSPYPYCHRHCRRKFYR			20	SLAY-screened peptide P4352			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGGCCTGACGTCCCCCTACCCCTATTGCCACAGGCATTGCCGGCGCAAGTTCTACCGGTAA	SGLTSPYPYCHRHCRRKFYR*	-1.288	0.023816	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26003	PIHSHTPLWSNRSSPWTTSM			20	SLAY-screened peptide P4353			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATCCATAGTCACACCCCTCTCTGGTCGAATCGGTCCTCCCCGTGGACGACGAGCATGTAA	PIHSHTPLWSNRSSPWTTSM*	-1.288	0.004885	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26004	TFAFHPHHTVTYVISPDHCG			20	SLAY-screened peptide P4354			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTTTGCTTTTCATCCGCATCATACTGTCACTTACGTGATCTCCCCTGACCACTGCGGTTAA	TFAFHPHHTVTYVISPDHCG*	-1.288	4.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26005	LYGNRCDQVIHVSDFPPTSA			20	SLAY-screened peptide P4355			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTATGGGAATAGGTGCGACCAGGTGATCCATGTTTCCGATTTTCCTCCTACCTCCGCCTAA	LYGNRCDQVIHVSDFPPTSA*	-1.288	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26006	CITYFVTLCSQAYSRRSRRL			20	SLAY-screened peptide P4356			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCATTACGTATTTCGTCACGCTCTGTTCTCAGGCCTACTCTCGTCGTTCGCGTCGTTTGTAA	CITYFVTLCSQAYSRRSRRL*	-1.287	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26007	SSDTNQ			6	SLAY-screened peptide P4357			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCAGTGACACTAACCAGTAGATTACCGGTTCTTAGCGGACCTCTTCGAACCGGGCTCGGTAA	SSDTNQ*ITGS*RTSSNRAR*	-1.287	0.001356	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26008	LFTTDYCRANRCPTDHVAER			20	SLAY-screened peptide P4358			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTCACTACTGACTATTGCCGCGCCAATCGCTGTCCGACCGACCACGTCGCGGAGCGTTAA	LFTTDYCRANRCPTDHVAER*	-1.287	0.008755	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26009	ARASSPRSRSNNPG			14	SLAY-screened peptide P4359			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCGGGCCAGTTCGCCCCGCTCCCGTAGCAACAACCCCGGTTAGGTCTACGGTGCCCCCTAA	ARASSPRSRSNNPG*VYGAP*	-1.287	0.01295	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26010	MMSPNKADQHSTLFLPALPC			20	SLAY-screened peptide P4360			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGATGTCGCCTAATAAGGCCGACCAGCATAGTACTCTCTTCTTGCCGGCCCTCCCGTGCTAA	MMSPNKADQHSTLFLPALPC*	-1.287	0.003386	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26011	CVYAVICIIVI			11	SLAY-screened peptide P4361			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGTGTACGCCGTTATCTGTATCATTGTAATATGACTGTTTTTTCTGCCAACTATTCGTAAC	CVYAVICIIVI*LFFLPTIRN	-1.286	7.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26012	SHTYHRALNPWIIRNNQVKP			20	SLAY-screened peptide P4362			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCACACCTACCATCGGGCCCTTAACCCCTGGATTATCCGTAATAACCAGGTCAAGCCTTAA	SHTYHRALNPWIIRNNQVKP*	-1.286	0.014305	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26013	DLHYYNPRLYCLSPAFALLD			20	SLAY-screened peptide P4363			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCTTCACTATTATAATCCGCGCTTGTACTGCTTGTCTCCGGCCTTTGCCCTCCTTGATTAA	DLHYYNPRLYCLSPAFALLD*	-1.286	0.011881	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26014	GRFVMMRHLLAVLKNVACGH			20	SLAY-screened peptide P4364			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCGGTTCGTTATGATGCGTCATCTTTTGGCCGTCTTGAAGAACGTCGCGTGCGGCCATTAA	GRFVMMRHLLAVLKNVACGH*	-1.286	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26015	TTPSIANSLMHMFLTRPGAP			20	SLAY-screened peptide P4365			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGACGCCCTCTATTGCGAACAGTCTTATGCATATGTTCTTGACCCGTCCCGGTGCTCCCTAA	TTPSIANSLMHMFLTRPGAP*	-1.286	0.000468	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26016	RRHFPVKMLHSTCMNFESLN			20	SLAY-screened peptide P4366			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGTCACTTTCCCGTCAAGATGCTTCATTCCACTTGCATGAATTTTGAGAGTCTGAATTAA	RRHFPVKMLHSTCMNFESLN*	-1.286	2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26017	SRNNISYTNLKSSNPAGLFH			20	SLAY-screened peptide P4367			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAGGAACAATATCTCTTACACTAATCTCAAGAGCTCTAACCCTGCGGGTCTTTTCCATTAA	SRNNISYTNLKSSNPAGLFH*	-1.285	0.024628	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26018	FLAPNPIPAPYRGASAHQLC			20	SLAY-screened peptide P4368			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCTGGCTCCTAATCCCATCCCTGCCCCTTATCGTGGGGCCTCGGCCCACCAGCTTTGCTAA	FLAPNPIPAPYRGASAHQLC*	-1.285	0.000122	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26019	HDWHMPTLFHPCNWAYNGYR			20	SLAY-screened peptide P4369			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGATTGGCACATGCCCACTCTTTTCCATCCTTGCAACTGGGCCTACAATGGCTACCGGTAA	HDWHMPTLFHPCNWAYNGYR*	-1.285	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26020	GCSDHLTSLYQRTLYTTTPW			20	SLAY-screened peptide P4370			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTGCAGTGATCATTTGACCAGTCTTTACCAGCGCACTCTCTACACCACCACTCCTTGGTAA	GCSDHLTSLYQRTLYTTTPW*	-1.285	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26021	RRYGASSQSSNLFNLVLFAI			20	SLAY-screened peptide P4371			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCGTTATGGTGCCTCGTCTCAGTCGTCCAACCTGTTCAATTTGGTGTTGTTCGCTATTTAA	RRYGASSQSSNLFNLVLFAI*	-1.284	0.038002	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26022	TAILARDVNHMTDLDKCFEV			20	SLAY-screened peptide P4372			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGCGATTCTTGCGCGTGATGTGAATCATATGACTGATCTTGACAAGTGTTTTGAGGTTTAA	TAILARDVNHMTDLDKCFEV*	-1.284	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26023	SITRLGSAASNSRTVNPART			20	SLAY-screened peptide P4373			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCATCACTCGGCTCGGCTCTGCCGCTAGTAACAGTAGGACTGTCAACCCGGCTCGCACTTAA	SITRLGSAASNSRTVNPART*	-1.284	0.013616	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26024	SCGPYYIVVRTYDNGLPLV			19	SLAY-screened peptide P4374			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTGTGGGCCGTACTACATCGTCGTTCGGACGTACGATAATGGTCTCCCCCTGGTTTAGTAA	SCGPYYIVVRTYDNGLPLV**	-1.284	0.000153	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26025	DPFPHANTGVRLFKGNTYNN			20	SLAY-screened peptide P4375			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCCGTTCCCCCACGCGAACACTGGCGTCAGGCTTTTTAAGGGGAACACTTACAACAACTAA	DPFPHANTGVRLFKGNTYNN*	-1.284	0.048108	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26026	MCRRIAIFTFRVCIIVGRRTN			21	SLAY-screened peptide P4376			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTGCCGGCGGATTGCTATTTTCACTTTCAGAGTTTGTATCATAGTTGGGAGGCGTACTAAC	MCRRIAIFTFRVCIIVGRRTN	-1.284	0.016004	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26027	PVNVHSGSRVTRTCTSPLFA			20	SLAY-screened peptide P4377			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGTTAACGTCCACTCGGGTTCCCGGGTTACTCGCACGTGCACCTCCCCGCTTTTTGCCTAA	PVNVHSGSRVTRTCTSPLFA*	-1.283	0.003781	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26028	YVSSSLATLGKNVLVSTYTD			20	SLAY-screened peptide P4378			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGTCTCTTCTAGTCTCGCCACTCTTGGGAAGAATGTTCTTGTGAGTACTTATACGGACTAA	YVSSSLATLGKNVLVSTYTD*	-1.283	0.005559	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26029	GVTIFHDNLTTCSTLDFCCW			20	SLAY-screened peptide P4379			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGTTACCATTTTCCATGACAACCTCACGACCTGTTCGACCCTCGACTTCTGTTGTTGGTAA	GVTIFHDNLTTCSTLDFCCW*	-1.283	0.001118	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26030	NSGLCNCIYWRADTKSPGMI			20	SLAY-screened peptide P4380			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTCTGGCTTGTGTAATTGTATTTACTGGCGGGCTGACACTAAGAGTCCGGGTATGATTTAA	NSGLCNCIYWRADTKSPGMI*	-1.282	0.011149	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26031	PDCTMYLMCNGISPVQRHVY			20	SLAY-screened peptide P4381			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGATTGCACCATGTACTTGATGTGTAACGGCATCAGTCCTGTTCAGCGCCATGTCTATTAA	PDCTMYLMCNGISPVQRHVY*	-1.282	0.000313	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26032	HCGNSPIITMPNGIHNYDAD			20	SLAY-screened peptide P4382			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGCGGTAATTCTCCGATTATCACCATGCCCAACGGTATTCATAATTACGACGCCGATTAA	HCGNSPIITMPNGIHNYDAD*	-1.282	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26033	KIANYHDNNHRTNH			14	SLAY-screened peptide P4383			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGATTGCCAATTACCATGACAACAACCACCGGACGAATCACTAGTGGTAGTTGGATCACTAA	KIANYHDNNHRTNH*W*LDH*	-1.282	0.015745	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26034	NATADLPATVHVLPWPTYTI			20	SLAY-screened peptide P4384			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGCCACTGCCGATCTTCCTGCTACCGTGCACGTCCTTCCCTGGCCCACTTATACGATTTAA	NATADLPATVHVLPWPTYTI*	-1.282	0.008315	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26035	NGIYRPPLVHLFKTPNTSDT			20	SLAY-screened peptide P4385			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGGCATCTATCGCCCTCCTCTCGTCCATCTTTTTAAGACTCCTAACACTAGTGACACGTAA	NGIYRPPLVHLFKTPNTSDT*	-1.282	0.004109	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26036	CFISPPLSVLYYLLRSLLDL			20	SLAY-screened peptide P4386			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTTCATTTCTCCCCCGTTGTCTGTGCTTTACTATCTGCTCCGCTCGCTCCTGGACCTGTAA	CFISPPLSVLYYLLRSLLDL*	-1.281	5.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26037	GPYDRLSKP			9	SLAY-screened peptide P4387			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCCCTACGACCGGCTTAGCAAGCCTTAGCCCCCCACTTACTATACCACTACGTACATTTAA	GPYDRLSKP*PPTYYTTTYI*	-1.281	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26038	GRAQNSEVMRPMHAFLVYYT			20	SLAY-screened peptide P4388			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCGTGCCCAGAATTCTGAGGTGATGAGGCCTATGCATGCTTTCCTCGTCTACTATACTTAA	GRAQNSEVMRPMHAFLVYYT*	-1.281	0.030375	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26039	FETPTTRYLLLPLDCSHPMH			20	SLAY-screened peptide P4389			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGAGACGCCTACCACCAGGTATCTCCTCCTCCCGCTGGACTGCTCTCATCCGATGCACTAA	FETPTTRYLLLPLDCSHPMH*	-1.281	0.000514	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26040	CPNLTPIASIPFKRRSNFSIN			21	SLAY-screened peptide P4390			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCCAACCTCACTCCTATTGCCTCGATCCCTTTTAAGAGGCGCTCCAACTTTAGTATTAAC	CPNLTPIASIPFKRRSNFSIN	-1.281	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26041	ASPFRFTPFTPTPRLIGALIN			21	SLAY-screened peptide P4391			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAGTCCTTTCAGGTTTACCCCCTTCACTCCAACACCCAGATTGATTGGAGCCTTAATTAAC	ASPFRFTPFTPTPRLIGALIN	-1.281	0.010294	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26042	LPGLDLFCRNHRPHP			15	SLAY-screened peptide P4392			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCCCGGGCTTGATCTTTTCTGCCGCAATCACCGTCCTCATCCTTAGCACAGGCGGCTCTAA	LPGLDLFCRNHRPHP*HRRL*	-1.281	0.000142	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26043	RSRSQLYSWSHMGHVYMSSK			20	SLAY-screened peptide P4393			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTCCAGGTCCCAGCTGTATTCCTGGTCCCACATGGGTCATGTCTACATGAGTAGCAAGTAA	RSRSQLYSWSHMGHVYMSSK*	-1.281	0.000619	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26044	KANPQVRTSTPRPTIAIATN			20	SLAY-screened peptide P4394			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGGCCAACCCGCAGGTGCGTACCTCTACTCCCCGGCCGACTATCGCCATCGCCACTAACTAA	KANPQVRTSTPRPTIAIATN*	-1.281	0.003866	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26045	RDSEPLYFVKDCGNYCLMRV			20	SLAY-screened peptide P4395			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGACAGCGAGCCGCTTTACTTTGTCAAGGATTGTGGTAATTATTGCCTCATGCGCGTGTAA	RDSEPLYFVKDCGNYCLMRV*	-1.28	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26046	PLHLTYLGIVRRVCSWLTNN			20	SLAY-screened peptide P4396			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTTGCACCTGACCTATCTTGGTATTGTTAGGCGGGTCTGCAGTTGGCTTACTAATAACTAA	PLHLTYLGIVRRVCSWLTNN*	-1.28	0.00897	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26047	SPWQIGADRIVPTMAWRMGT			20	SLAY-screened peptide P4397			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCCCTGGCAGATCGGCGCCGATAGGATCGTTCCTACCATGGCGTGGCGGATGGGCACTTAA	SPWQIGADRIVPTMAWRMGT*	-1.28	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26048	AVSVFHLTLHNLKPSKFVYW			20	SLAY-screened peptide P4398			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGTCAGCGTCTTCCATCTTACTTTGCACAATCTCAAGCCCTCGAAGTTCGTGTATTGGTAA	AVSVFHLTLHNLKPSKFVYW*	-1.28	0.002493	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26049	TVAQHIRVSPGFCV			14	SLAY-screened peptide P4399			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGTCGCCCAGCATATCAGGGTCAGTCCCGGTTTTTGCGTGTAGCTGGCTTGCCACAAGTAA	TVAQHIRVSPGFCV*LACHK*	-1.279	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26050	PNMPQNSEQSKNSSLSTQNM			20	SLAY-screened peptide P4400			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAACATGCCCCAGAATTCTGAGCAGAGTAAGAACTCGAGTCTCAGCACTCAGAACATGTAA	PNMPQNSEQSKNSSLSTQNM*	-1.279	0.019876	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26051	FSDICKFCQGYYALERPCVL			20	SLAY-screened peptide P4401			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAGCGACATCTGTAAGTTTTGCCAGGGCTATTATGCCTTGGAGCGGCCGTGCGTTTTGTAA	FSDICKFCQGYYALERPCVL*	-1.279	0.001021	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26052	TSHYAPIIMVSI			12	SLAY-screened peptide P4402			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAGTCATTATGCTCCCATCATTATGGTTAGTATTTAGACCCCTAGTAACAAGGGCTGCTAA	TSHYAPIIMVSI*TPSNKGC*	-1.279	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26053	PFLL			4	SLAY-screened peptide P4403			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTCCTGCTGTAGTCGCCGCCCACGCTCTTCTGGTCTACTACTTAGAAGCCGGCTACGTAA	PFLL*SPPTLFWSTT*KPAT*	-1.278	0.003494	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26054	CARVSAKTSSTSWTLPHRAA			20	SLAY-screened peptide P4404			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCCCGCGTGAGCGCCAAGACGTCCAGCACGTCCTGGACTCTCCCCCATCGTGCTGCTTAA	CARVSAKTSSTSWTLPHRAA*	-1.278	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26055	TMQWSPLRMPRRATTRCGAQ			20	SLAY-screened peptide P4405			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCATGCAGTGGTCGCCGCTCCGTATGCCCCGCCGCGCCACTACGCGCTGCGGGGCCCAGTAA	TMQWSPLRMPRRATTRCGAQ*	-1.278	0.044551	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26056	RWLNQMNHLSNSSCLAH			17	SLAY-screened peptide P4406			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGGTTGAACCAGATGAACCACCTCAGTAATTCGTCGTGCCTGGCCCATTAGCTCAATTAA	RWLNQMNHLSNSSCLAH*LN*	-1.278	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26057	SRAMPSSAPRPEERQPRDTV			20	SLAY-screened peptide P4407			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCGGGCTATGCCTAGTAGCGCTCCTAGGCCGGAGGAGAGGCAGCCGAGGGACACCGTCTAA	SRAMPSSAPRPEERQPRDTV*	-1.278	0.044647	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26058	SWYTTVVFAFNLLNCLRVFIN			21	SLAY-screened peptide P4408			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTGGTATACCACTGTGGTTTTTGCCTTTAACCTCCTGAACTGCTTAAGGGTATTTATTAAC	SWYTTVVFAFNLLNCLRVFIN	-1.278	0.002672	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26059	HHNTCQCLYNTMSDIYHASA			20	SLAY-screened peptide P4409			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCACAATACCTGCCAGTGCCTTTACAACACCATGTCCGATATTTATCACGCTAGCGCCTAA	HHNTCQCLYNTMSDIYHASA*	-1.278	0.006118	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26060	HAVYDMSFGIADRFVSRQSA			20	SLAY-screened peptide P4410			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCTGTTTACGATATGAGCTTCGGTATTGCTGATAGGTTTGTGTCGCGCCAGAGCGCCTAA	HAVYDMSFGIADRFVSRQSA*	-1.278	0.005764	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26061	VFLVVLIISTGVLPTRTIRCN			21	SLAY-screened peptide P4411			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTTTTTAGTCGTATTAATAATAAGCACCGGTGTATTGCCGACTAGGACTATTCGGTGTAAC	VFLVVLIISTGVLPTRTIRCN	-1.277	0.001282	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26062	KDSYFLTIAVYYYAHPESPS			20	SLAY-screened peptide P4412			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGGATTCCTATTTCTTGACTATCGCTGTCTACTATTACGCCCATCCGGAGTCGCCTTCGTAA	KDSYFLTIAVYYYAHPESPS*	-1.277	0.01044	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26063	LAHSRNPYT			9	SLAY-screened peptide P4413			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGCGCACAGTCGCAACCCCTACACTTAGGCGGCCCAGAGGCGGACTAGCGACGTTAAGTAA	LAHSRNPYT*AAQRRTSDVK*	-1.277	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26064	LCPRHGAGITIFRLRLFN			18	SLAY-screened peptide P4414			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTGCCCGAGGCATGGCGCCGGTATTACCATATTTCGCTTACGGCTATTTAACTGAGTAAGT	LCPRHGAGITIFRLRLFN*VS	-1.277	0.004293	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26065	HCGPQANASHVHNIIPILNM			20	SLAY-screened peptide P4415			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGTGGCCCTCAGGCTAACGCTAGCCATGTCCACAATATCATCCCCATTTTGAACATGTAA	HCGPQANASHVHNIIPILNM*	-1.277	0.042712	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26066	SGYGQPRDPNNKTM			14	SLAY-screened peptide P4416			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGGCTATGGTCAGCCCCGGGACCCTAACAATAAGACCATGTAGTATGTTTCCTAACTGAGT	SGYGQPRDPNNKTM*YVS*LS	-1.277	0.036831	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26067	TPPISLCWASRPASNPSPGS			20	SLAY-screened peptide P4417			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCCCCCTATCTCGCTCTGCTGGGCCAGTCGGCCTGCTAGCAATCCCAGCCCGGGCTCCTAA	TPPISLCWASRPASNPSPGS*	-1.276	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26068	RILRFLRVFLTSRPCARVSRP			21	SLAY-screened peptide P4418			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTATCCTACGTTTTCTAAGAGTTTTCCTTACTAGTAGACCATGCGCACGAGTAAGTCGACCT	RILRFLRVFLTSRPCARVSRP	-1.276	0.000917	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26069	SRSSAAACCNYEAFSIPILL			20	SLAY-screened peptide P4419			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCGCTCTTCCGCTGCGGCGTGTTGTAATTACGAGGCTTTTTCTATCCCGATTCTCTTGTAA	SRSSAAACCNYEAFSIPILL*	-1.276	9.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26070	PSRRGFGLYVQVFNVRRRTD			20	SLAY-screened peptide P4420			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAGTAGGCGTGGTTTCGGCCTCTATGTGCAGGTGTTTAACGTTCGGCGTCGGACCGACTAA	PSRRGFGLYVQVFNVRRRTD*	-1.276	0.020996	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26071	TALLTTPVRTTSSTDTFFNS			20	SLAY-screened peptide P4421			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGCGCTTCTTACTACCCCGGTTCGCACCACGAGCAGCACGGACACGTTTTTTAACTCCTAA	TALLTTPVRTTSSTDTFFNS*	-1.275	0.001363	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26072	DLGDPLDNITWCMSNSTYNN			20	SLAY-screened peptide P4422			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCTTGGGGATCCTCTCGATAATATTACCTGGTGTATGTCTAATTCCACTTATAATAACTAA	DLGDPLDNITWCMSNSTYNN*	-1.275	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26073	VHLLGNSMNGGCTASVHPVV			20	SLAY-screened peptide P4423			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCATCTGCTCGGCAATTCGATGAATGGTGGTTGTACTGCTTCCGTCCATCCTGTGGTGTAA	VHLLGNSMNGGCTASVHPVV*	-1.275	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26074	GLPPANPALHAIIVPCVPII			20	SLAY-screened peptide P4424			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTTGCCTCCGGCGAACCCCGCTCTTCACGCGATTATTGTGCCGTGTGTTCCCATTATTTAA	GLPPANPALHAIIVPCVPII*	-1.275	8.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26075	TISSSARGCT			10	SLAY-screened peptide P4425			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGATTTCTAGCTCAGCGCGCGGGTGCACATGAGTTAGACTATCGACTATCTGCATTCGTAAC	TISSSARGCT*VRLSTICIRN	-1.275	0.001048	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26076	AYIHPSGPTDDNPCQTPRYT			20	SLAY-screened peptide P4426			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTATATCCACCCCAGCGGGCCTACTGACGATAACCCGTGCCAGACTCCGAGGTACACCTAA	AYIHPSGPTDDNPCQTPRYT*	-1.275	0.000101	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26077	CTAYLYGTPDASRCRSCCYT			20	SLAY-screened peptide P4427			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCACGGCCTATCTTTACGGGACCCCTGATGCCAGTCGGTGCCGTAGTTGTTGCTATACTTAA	CTAYLYGTPDASRCRSCCYT*	-1.275	0.010244	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26078	RQNLSFARYFPYGVVH			16	SLAY-screened peptide P4428			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCAGAACCTGAGTTTCGCGCGTTACTTTCCGTACGGAGTTGTCCATTAATAGCTCGTAACT	RQNLSFARYFPYGVVH**LVT	-1.274	0.012727	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26079	VLRPR			5	SLAY-screened peptide P4429			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTTGCGCCCCCGTTAGCCCGTCTCTCGTCACGTCTGCACTTACAATCGGTATTTGCTCTAA	VLRPR*PVSRHVCTYNRYLL*	-1.274	0.027784	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26080	TLVTPPGFAFVPSILLHRHA			20	SLAY-screened peptide P4430			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTCGTTACTCCGCCGGGTTTCGCTTTTGTTCCCAGCATTCTTCTTCATCGTCACGCTTAA	TLVTPPGFAFVPSILLHRHA*	-1.274	0.011981	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26081	HSDLPSYHVLPHVLIHVVLA			20	SLAY-screened peptide P4431			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTCCGACCTGCCGTCTTATCATGTCCTGCCTCATGTTCTTATCCATGTCGTTCTGGCCTAA	HSDLPSYHVLPHVLIHVVLA*	-1.274	0.0198	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26082	PTGTKGLEHTPMNPFDISLI			20	SLAY-screened peptide P4432			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACCGGGACCAAGGGCCTGGAGCACACGCCCATGAACCCTTTTGACATTTCCCTTATCTAA	PTGTKGLEHTPMNPFDISLI*	-1.273	0.004925	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26083	LTTDSGWSSGQVDSGLLTRS			20	SLAY-screened peptide P4433			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGACTACGGACTCGGGGTGGAGTTCGGGCCAGGTCGACTCGGGTCTCCTTACCCGTAGTTAA	LTTDSGWSSGQVDSGLLTRS*	-1.273	0.001082	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26084	RDDNCSRWPMPENTLRLRRCN			21	SLAY-screened peptide P4434			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGATGATAACTGTAGCAGGTGGCCTATGCCCGAGAACACTTTGCGCCTCAGGCGATGTAAC	RDDNCSRWPMPENTLRLRRCN	-1.273	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26085	HHSVNNGWRAYL			12	SLAY-screened peptide P4435			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCACAGCGTCAACAACGGTTGGCGCGCTTACCTCTAGAGTAATGCTCATAATTGCTATTAA	HHSVNNGWRAYL*SNAHNCY*	-1.273	7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26086	PQVRAVAPLGISCA			14	SLAY-screened peptide P4436			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCAGGTGCGGGCCGTCGCCCCCCTTGGGATAAGCTGCGCTTAGGCGCGATAAGTGTTAACT	PQVRAVAPLGISCA*AR*VLT	-1.273	0.002957	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26087	YCTYYT			6	SLAY-screened peptide P4437			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTGTACCTATTATACTTAGCACTACAGGCTTCTTTACAATATCATGAATTAGGCCGACTAA	YCTYYT*HYRLLYNIMN*AD*	-1.273	0.004303	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26088	NRHFRHLCATDFNGSAFQNS			20	SLAY-screened peptide P4438			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCGCCACTTTCGTCATCTTTGTGCCACGGATTTCAATGGTAGTGCGTTCCAGAATTCTTAA	NRHFRHLCATDFNGSAFQNS*	-1.273	0.000845	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26089	SCHRYGSGDYLKTLWRYSYF			20	SLAY-screened peptide P4439			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTGTCACCGTTATGGCTCGGGCGATTATTTGAAGACGTTGTGGCGGTACAGTTATTTTTAA	SCHRYGSGDYLKTLWRYSYF*	-1.273	0.012728	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26090	VYIILTDPGLL			11	SLAY-screened peptide P4440			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTACATTATTCTTACTGACCCTGGTCTGCTTTAGGGGGGCCCCAAGGCCAATAATCCGTAA	VYIILTDPGLL*GGPKANNP*	-1.273	0.000846	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26091	SFHTRPA			7	SLAY-screened peptide P4441			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTTCCATACCAGGCCCGCTTAGGGTACCAGCCTTCAGTCGGATAACTCGTCGCGCAATTAA	SFHTRPA*GTSLQSDNSSRN*	-1.273	0.003768	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26092	YPSCFCNTRNICQTMQEEII			20	SLAY-screened peptide P4442			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCCTTCGTGTTTCTGCAATACGCGTAACATTTGTCAGACCATGCAGGAGGAGATTATCTAA	YPSCFCNTRNICQTMQEEII*	-1.273	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26093	PTTCTWLPTDYHQCNCHVFW			20	SLAY-screened peptide P4443			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACCACTTGCACCTGGCTTCCCACGGACTACCACCAGTGTAACTGTCATGTCTTCTGGTAA	PTTCTWLPTDYHQCNCHVFW*	-1.272	0.021561	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26094	RAEP			4	SLAY-screened peptide P4444			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGCCGAGCCTTAGTCCATTTTTCACGCTACCACCAAGACTGTTCACTTCTACGGCCGCTAA	RAEP*SIFHATTKTVHFYGR*	-1.272	0.000167	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26095	RPDSTHIDHSYGCIYITT			18	SLAY-screened peptide P4445			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCGGATAGCACTCACATTGATCACTCGTACGGCTGCATCTATATTACCACTTAGGACTAA	RPDSTHIDHSYGCIYITT*D*	-1.272	0.006936	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26096	PLPHNTKCRLLPLPFRPRPD			20	SLAY-screened peptide P4446			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTGCCGCACAACACCAAGTGCCGCCTCCTCCCCTTGCCTTTCCGGCCGCGCCCGGACTAA	PLPHNTKCRLLPLPFRPRPD*	-1.272	0.015654	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26097	LRILQPPSASYPHDFASRLR			20	SLAY-screened peptide P4447			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCGCATTCTGCAGCCGCCCAGTGCCAGCTATCCTCATGACTTCGCCAGCCGCCTGCGGTAA	LRILQPPSASYPHDFASRLR*	-1.272	0.001069	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26098	HPWCPITQHSVYIYNPHTAN			20	SLAY-screened peptide P4448			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCGTGGTGCCCTATTACGCAGCACTCGGTCTATATTTATAACCCCCATACTGCTAACTAA	HPWCPITQHSVYIYNPHTAN*	-1.272	0.033331	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26099	LVTDCHLSKSTPATLRLIQDH			21	SLAY-screened peptide P4449			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGTCACGGACTGCCACCTGAGTAAGTCGACCCCAGCTACGCTGCGCCTCATCCAGGATCAT	LVTDCHLSKSTPATLRLIQDH	-1.272	0.016572	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26100	GCPPLSPWYCLVAAHYRLYC			20	SLAY-screened peptide P4450			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTGCCCCCCGCTGTCTCCGTGGTACTGTTTGGTCGCTGCGCACTACAGGCTTTATTGCTAA	GCPPLSPWYCLVAAHYRLYC*	-1.272	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26101	TTIFWTRG			8	SLAY-screened peptide P4451			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGACGATTTTTTGGACTCGGGGCTAGGTGGACCGCGCCTTCCTTACCCTTGTTAGTGGTTAC	TTIFWTRG*VDRAFLTLVSGY	-1.272	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26102	WRNLPDGTWNYVSDLNAPFQ			20	SLAY-screened peptide P4452			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCGGAACCTTCCCGATGGTACGTGGAACTACGTTTCGGATCTCAATGCTCCGTTTCAGTAA	WRNLPDGTWNYVSDLNAPFQ*	-1.271	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26103	QNPDLYLHYLINMNPKAYVS			20	SLAY-screened peptide P4453			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGAACCCGGACCTTTACTTGCATTATCTGATCAACATGAATCCGAAGGCGTACGTCTCGTAA	QNPDLYLHYLINMNPKAYVS*	-1.271	0.004583	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26104	CWCRARNPLYDLVYHTSS			18	SLAY-screened peptide P4454			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTGGTGTCGGGCCCGCAACCCGCTTTACGACTTGGTTTACCACACCAGCTCTTAGAGCTAA	CWCRARNPLYDLVYHTSS*S*	-1.271	0.008738	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26105	SDIHNAVDLCARPLVLLLLGQ			21	SLAY-screened peptide P4455			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGACATTCATAACGCTGTTGACCTTTGCGCCCGCCCTCTCGTGCTCCTGCTTCTAGGACAA	SDIHNAVDLCARPLVLLLLGQ	-1.271	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26106	HDDSFCFRYVYRRPRQGPAA			20	SLAY-screened peptide P4456			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGATGACTCCTTCTGTTTCAGGTACGTGTATAGGCGTCCTAGGCAGGGGCCGGCTGCTTAA	HDDSFCFRYVYRRPRQGPAA*	-1.27	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26107	GACIYMRICTASPRNILCPN			20	SLAY-screened peptide P4457			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGGCCTGTATTTATATGCGTATCTGTACCGCGAGTCCCCGTAATATCCTGTGCCCGAACTAA	GACIYMRICTASPRNILCPN*	-1.27	0.000128	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26108	APPPRLAHNRTLRCGKT			17	SLAY-screened peptide P4458			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCCCCCCCCTCGCCTGGCCCATAATAGGACCCTCCGGTGTGGCAAGACTTAGTACAGCTAA	APPPRLAHNRTLRCGKT*YS*	-1.269	0.013623	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26109	PCYRAFHHYIKDLILGLVRSN			21	SLAY-screened peptide P4459			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGCTATCGCGCTTTTCACCACTACATCAAGGATTTAATACTAGGCCTTGTTCGCAGTAAC	PCYRAFHHYIKDLILGLVRSN	-1.269	0.004305	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26110	PLVRYDQRTSPLHHETIVNI			20	SLAY-screened peptide P4460			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTGGTGCGCTATGATCAGCGCACTAGTCCCCTGCATCACGAGACCATTGTCAACATCTAA	PLVRYDQRTSPLHHETIVNI*	-1.269	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26111	IPCGYTMTSYIRFDTHRNAF			20	SLAY-screened peptide P4461			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCCGTGTGGCTATACTATGACCAGTTACATTCGGTTTGATACCCATCGCAATGCGTTTTAA	IPCGYTMTSYIRFDTHRNAF*	-1.269	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26112	TFQCTDSATPNLTPPFLLI			19	SLAY-screened peptide P4462			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTTCCAGTGTACGGACTCGGCCACTCCTAATCTGACCCCCCCTTTTCTTCTTATCTAGTAA	TFQCTDSATPNLTPPFLLI**	-1.269	0.000288	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26113	TQCDHCPYNKPLSPDFVHSR			20	SLAY-screened peptide P4463			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCAGTGCGACCACTGCCCTTATAACAAGCCTCTTAGTCCTGACTTCGTCCATTCTCGCTAA	TQCDHCPYNKPLSPDFVHSR*	-1.269	0.00088	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26114	LLYFNR			6	SLAY-screened peptide P4464			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTTTACTTTAACCGCTAGGATCGGCTTTGTGGGTGGGATCATTTGTTGCTCGACTTCTAA	LLYFNR*DRLCGWDHLLLDF*	-1.268	0.000435	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26115	ADQEISARQFLHDLCHSGQL			20	SLAY-screened peptide P4465			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGACCAGGAGATCAGCGCTAGGCAGTTTTTGCACGATCTTTGCCATTCGGGTCAGCTGTAA	ADQEISARQFLHDLCHSGQL*	-1.268	0.000599	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26116	HNHLYHDTCTLNRRNCEAHG			20	SLAY-screened peptide P4466			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAACCATCTCTACCATGACACCTGTACTTTGAACCGTCGGAATTGTGAGGCCCACGGGTAA	HNHLYHDTCTLNRRNCEAHG*	-1.267	0.00313	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26117	RSINYITIHPLSTRIFIPAP			20	SLAY-screened peptide P4467			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCGATTAATTACATTACTATTCATCCCCTCAGTACTCGTATCTTCATCCCTGCTCCGTAA	RSINYITIHPLSTRIFIPAP*	-1.267	0.00272	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26118	PYDSGYTKVHAIIGTTSNQT			20	SLAY-screened peptide P4468			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTATGACTCTGGTTACACCAAGGTTCACGCCATCATTGGTACCACCTCCAATCAGACTTAA	PYDSGYTKVHAIIGTTSNQT*	-1.267	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26119	VPHHSYFSSRRI			12	SLAY-screened peptide P4469			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCCGCATCATAGTTACTTTTCTTCGCGGAGGATCTAGTTTACTAGTATTGTCTGTTACTAA	VPHHSYFSSRRI*FTSIVCY*	-1.267	0.030785	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26120	WPTSHTRSLLAYNYQCNGT			19	SLAY-screened peptide P4470			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCCTACCTCTCATACGAGGTCCCTTTTGGCCTACAACTACCAGTGTAACGGGACTTAGTAA	WPTSHTRSLLAYNYQCNGT**	-1.267	0.00073	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26121	AWRHQVIAPLYYLNFYTGSGN			21	SLAY-screened peptide P4471			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTGGCGTCACCAGGTGATCGCCCCTCTGTACTACCTCAATTTCTACACAGGTAGCGGTAAC	AWRHQVIAPLYYLNFYTGSGN	-1.266	0.00012	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26122	SANAPFCFLCTCPYTITHTI			20	SLAY-screened peptide P4472			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGCCAATGCCCCGTTCTGCTTCCTTTGCACTTGCCCTTATACGATTACCCATACTATCTAA	SANAPFCFLCTCPYTITHTI*	-1.265	0.02747	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26123	TSSRRRIWVMLISLFLTLFAN			21	SLAY-screened peptide P4473			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCTAGTCGCAGGAGAATCTGGGTGATGTTGATATCACTGTTCTTAACTCTATTTGCTAAC	TSSRRRIWVMLISLFLTLFAN	-1.265	0.000717	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26124	FVIHPVFHVPPVVTFGPFSL			20	SLAY-screened peptide P4474			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGTCATTCACCCTGTGTTTCACGTGCCCCCCGTGGTGACCTTTGGCCCCTTCAGTTTGTAA	FVIHPVFHVPPVVTFGPFSL*	-1.265	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26125	GFADCLPIYGLLHPYYKFHI			20	SLAY-screened peptide P4475			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGTTCGCGGATTGCCTGCCTATTTATGGCCTTCTCCATCCTTACTATAAGTTTCACATTTAA	GFADCLPIYGLLHPYYKFHI*	-1.265	0.002373	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26126	AHLRELSGLNPRNHARSNPF			20	SLAY-screened peptide P4476			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCACCTGCGCGAGCTCTCTGGGCTGAACCCTCGCAACCATGCTCGCTCCAACCCGTTTTAA	AHLRELSGLNPRNHARSNPF*	-1.264	0.00068	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26127	LSMFRCPDTYPDHFLELTLR			20	SLAY-screened peptide P4477			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTCCATGTTCCGTTGCCCGGACACTTATCCGGACCACTTCCTGGAGTTGACCCTCCGGTAA	LSMFRCPDTYPDHFLELTLR*	-1.264	0.015898	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26128	PGTPKCTAVVYVSSLAMRC			19	SLAY-screened peptide P4478			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGTACTCCCAAGTGTACCGCTGTTGTGTATGTCAGTTCTCTCGCTATGAGGTGTTAGTAA	PGTPKCTAVVYVSSLAMRC**	-1.264	0.026167	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26129	YSCAGLPHQFYCHYTCNQPL			20	SLAY-screened peptide P4479			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACAGTTGTGCTGGCCTGCCCCATCAGTTCTATTGCCATTATACGTGCAATCAGCCCTTGTAA	YSCAGLPHQFYCHYTCNQPL*	-1.264	0.006986	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26130	PPSSRSHHRRLPASCLATFI			20	SLAY-screened peptide P4480			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCTCGTCGCGCAGCCACCACCGCCGGCTTCCTGCCTCCTGCTTGGCCACCTTTATCTAA	PPSSRSHHRRLPASCLATFI*	-1.264	0.006113	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26131	NMCPWMPPLLL			11	SLAY-screened peptide P4481			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACATGTGTCCCTGGATGCCACCGCTACTACTATGAACATCGCTGCCTTCGTGTAGCTCTAAC	NMCPWMPPLLL*TSLPSCSSN	-1.263	0.000142	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26132	LMPTPSVHASIPRGNPIIRTN			21	SLAY-screened peptide P4482			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATGCCTACGCCGTCTGTCCATGCCTCGATTCCGAGGGGGAACCCTATCATACGAACTAAC	LMPTPSVHASIPRGNPIIRTN	-1.263	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26133	YFLNPMSIYHFLTLHCLNYA			20	SLAY-screened peptide P4483			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTTTCTGAACCCCATGAGTATTTATCACTTTCTCACGCTTCACTGTTTGAACTATGCGTAA	YFLNPMSIYHFLTLHCLNYA*	-1.263	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26134	GPLYDKFSLEPRVTLEFYAH			20	SLAY-screened peptide P4484			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCCCCTCTACGACAAGTTCAGCCTTGAGCCTCGTGTCACCCTCGAGTTCTATGCGCACTAA	GPLYDKFSLEPRVTLEFYAH*	-1.262	0.000317	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26135	TPGLIILCPTHGSTSLNPRS			20	SLAY-screened peptide P4485			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCCGGTCTGATCATCTTGTGCCCTACCCACGGGAGTACTAGCCTTAATCCGCGCTCGTAA	TPGLIILCPTHGSTSLNPRS*	-1.262	0.012345	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26136	CPSPTVAPNPYTVKY			15	SLAY-screened peptide P4486			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCTAGTCCTACTGTGGCTCCGAATCCGTACACGGTTAAGTACTAGAGCATTGGCACTAAC	CPSPTVAPNPYTVKY*SIGTN	-1.261	3.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26137	PQPVPFEDVLDCAARLYTPS			20	SLAY-screened peptide P4487			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCAGCCTGTTCCGTTCGAGGACGTTCTCGATTGCGCTGCCCGGCTCTATACGCCGAGTTAA	PQPVPFEDVLDCAARLYTPS*	-1.261	1.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26138	PMCRSHLNDPFQLILYRTAD			20	SLAY-screened peptide P4488			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATGTGCCGTTCTCACCTTAACGACCCCTTCCAGCTCATCCTTTATCGCACCGCGGACTAA	PMCRSHLNDPFQLILYRTAD*	-1.26	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26139	PIRPNPPVCMSHPMWLNDTA			20	SLAY-screened peptide P4489			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATCCGGCCCAATCCCCCCGTCTGTATGAGCCACCCCATGTGGCTCAATGACACGGCCTAA	PIRPNPPVCMSHPMWLNDTA*	-1.26	5.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26140	QTTIHCPRVNPFTLPIPFCS			20	SLAY-screened peptide P4490			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGACTACCATTCATTGCCCGCGTGTTAACCCGTTCACGCTCCCTATTCCGTTTTGCTCGTAA	QTTIHCPRVNPFTLPIPFCS*	-1.26	0.00945	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26141	LNLHCYTSVGHDIPRNGSDN			20	SLAY-screened peptide P4491			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGAACTTGCACTGTTACACGTCTGTGGGTCACGATATCCCCCGGAATGGCTCTGACAACTAA	LNLHCYTSVGHDIPRNGSDN*	-1.26	0.03057	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26142	TIGHCSE			7	SLAY-screened peptide P4492			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGATTGGTCATTGCTCGGAGTAGGATCACGAGTTTAATAGGCCTTCGTACAGTTTTACTAAC	TIGHCSE*DHEFNRPSYSFTN	-1.26	0.016058	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26143	CSLRCLS			7	SLAY-screened peptide P4493			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAGCCTTAGGTGTCTGTCGTAGTCGGGGACCAATTCTGTTTTGGAGCCGGCCGGCAACTAA	CSLRCLS*SGTNSVLEPAGN*	-1.26	0.004616	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26144	PNLPIIFIGRCYRRSRIYRP			20	SLAY-screened peptide P4494			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAACCTGCCTATTATCTTTATTGGGCGTTGTTACCGGAGGAGCCGCATCTATCGGCCTTAA	PNLPIIFIGRCYRRSRIYRP*	-1.259	0.001088	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26145	LRRGQPFTACTLGNNSAYAL			20	SLAY-screened peptide P4495			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCGTCGCGGCCAGCCTTTTACGGCTTGCACCCTGGGTAACAACTCCGCCTACGCCCTCTAA	LRRGQPFTACTLGNNSAYAL*	-1.259	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26146	SSLCCANNLLRAPESSPARR			20	SLAY-screened peptide P4496			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTCGCTCTGCTGTGCCAACAATCTGCTTCGTGCTCCCGAGAGTAGTCCGGCGCGCAGGTAA	SSLCCANNLLRAPESSPARR*	-1.259	0.005693	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26147	TPCIHHNLPWIVRLFSLLGS			20	SLAY-screened peptide P4497			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCCTTGTATCCATCATAACCTCCCCTGGATCGTCCGCCTTTTTTCTCTCCTCGGTTCTTAA	TPCIHHNLPWIVRLFSLLGS*	-1.259	0.014624	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26148	APPTTVLATTTRRRLRSLRRN			21	SLAY-screened peptide P4498			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCACCTACCACCGTCCTGGCTACAACTACAAGACGCCGACTCCGCTCCCTTCGGCGTAAC	APPTTVLATTTRRRLRSLRRN	-1.259	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26149	DCFTSFDFVLNY			12	SLAY-screened peptide P4499			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTGCTTCACTTCGTTTGATTTTGTCCTGAATTATTAGCCTGCTCTCACTACCGCTCGCTAA	DCFTSFDFVLNY*PALTTAR*	-1.259	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26150	PLLVPYAPCYTVAIPINLPK			20	SLAY-screened peptide P4500			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTGCTTGTTCCGTATGCCCCCTGTTACACTGTTGCCATTCCCATTAATCTGCCCAAGTAA	PLLVPYAPCYTVAIPINLPK*	-1.258	0.000116	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26151	TSPNCSPNLNTGRYLSYDLI			20	SLAY-screened peptide P4501			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTCGCCCAACTGTAGCCCCAATTTGAACACTGGTCGCTATCTCTCTTATGACTTGATTTAA	TSPNCSPNLNTGRYLSYDLI*	-1.258	0.048467	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26152	NIHHNYCDVPRAYKNLSGIL			20	SLAY-screened peptide P4502			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACATCCACCACAACTACTGCGACGTGCCGCGTGCGTATAAGAATCTGTCCGGGATTCTGTAA	NIHHNYCDVPRAYKNLSGIL*	-1.258	0.000241	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26153	TCSQDSSVLNNAHIILVSGT			20	SLAY-screened peptide P4503			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTGTTCGCAGGACTCCAGCGTCCTGAATAATGCCCACATTATCCTGGTCAGTGGTACTTAA	TCSQDSSVLNNAHIILVSGT*	-1.258	0.015229	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26154	RYHSLPAWSAIMCPIARPLFP			21	SLAY-screened peptide P4504			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTACCACTCTTTGCCCGCTTGGAGCGCTATCATGTGCCCTATTGCGCGCCCATTGTTTCCG	RYHSLPAWSAIMCPIARPLFP	-1.258	0.016124	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26155	TINPRPSLRAIKNGAVYSAF			20	SLAY-screened peptide P4505			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCATTAACCCCCGTCCCAGTCTTCGTGCGATTAAGAACGGTGCGGTTTACAGTGCGTTTTAA	TINPRPSLRAIKNGAVYSAF*	-1.258	0.003518	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26156	FFDCTNVNGAHSLFYLTNPI			20	SLAY-screened peptide P4506			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTTCGACTGCACTAATGTTAATGGCGCTCATAGTCTTTTCTACCTTACGAATCCCATTTAA	FFDCTNVNGAHSLFYLTNPI*	-1.258	0.016016	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26157	SCKTMLNHKLMDHITSLFMH			20	SLAY-screened peptide P4507			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTGCAAGACGATGCTTAACCATAAGCTTATGGACCACATTACGAGCTTGTTTATGCATTAA	SCKTMLNHKLMDHITSLFMH*	-1.258	0.000202	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26158	ATRVDPQCWRYYLLPIGNVL			20	SLAY-screened peptide P4508			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGACGCGCGTGGATCCTCAGTGCTGGCGCTATTATCTGCTTCCCATTGGTAACGTGCTTTAA	ATRVDPQCWRYYLLPIGNVL*	-1.258	0.008782	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26159	YNPCSLLCSRYTNCPFTNRD			20	SLAY-screened peptide P4509			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACAATCCTTGCAGTCTGCTTTGTTCCCGGTATACGAACTGCCCCTTTACGAACCGCGACTAA	YNPCSLLCSRYTNCPFTNRD*	-1.258	0.000753	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26160	ISGMPSLLPYRYRRKRTASH			20	SLAY-screened peptide P4510			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTAGCGGCATGCCGTCTCTTCTGCCTTACCGTTATCGGCGCAAGCGGACTGCTTCCCACTAA	ISGMPSLLPYRYRRKRTASH*	-1.258	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26161	VHDDAPTSIYADCTTLISLV			20	SLAY-screened peptide P4511			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCATGACGATGCGCCGACTTCGATTTATGCCGACTGCACTACGTTGATCTCCCTTGTTTAA	VHDDAPTSIYADCTTLISLV*	-1.257	0.003866	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26162	HPDAHSAGHRITPVLWPIQA			20	SLAY-screened peptide P4512			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCCGACGCGCACTCTGCCGGGCATCGTATTACCCCGGTCCTTTGGCCGATCCAGGCTTAA	HPDAHSAGHRITPVLWPIQA*	-1.257	0.0408	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26163	GHLVSFFP			8	SLAY-screened peptide P4513			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCACCTGGTTTCGTTTTTTCCTTAGTTGGGGCCGTACGCCATCTCGTCTTCCAGTACTTAA	GHLVSFFP*LGPYAISSSST*	-1.257	2.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26164	ASMTHNTKFTSFRRIMTTSR			20	SLAY-screened peptide P4514			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTCTATGACTCACAACACCAAGTTTACCAGTTTTCGTCGCATCATGACCACGTCGCGCTAA	ASMTHNTKFTSFRRIMTTSR*	-1.257	0.002704	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26165	PNMLYNNNNTQTGSTPYATL			20	SLAY-screened peptide P4515			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAACATGCTCTACAACAATAACAATACCCAGACGGGTAGCACCCCCTACGCTACGCTCTAA	PNMLYNNNNTQTGSTPYATL*	-1.256	5.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26166	KTSKLIDGNSMRVNSNIGRF			20	SLAY-screened peptide P4516			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGACGTCCAAGCTTATTGATGGCAACTCGATGCGTGTTAACTCTAATATCGGGCGTTTTTAA	KTSKLIDGNSMRVNSNIGRF*	-1.256	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26167	EELVMLMGVGVINMHHVHMC			20	SLAY-screened peptide P4517			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGAGTTGGTTATGTTGATGGGCGTCGGTGTGATTAATATGCATCACGTTCATATGTGTTAA	EELVMLMGVGVINMHHVHMC*	-1.256	0.026433	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26168	VMELISVLYPPLRSRLTGVT			20	SLAY-screened peptide P4518			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCATGGAGCTCATCAGCGTTCTTTACCCCCCTCTTCGTTCTCGTCTCACCGGGGTTACTTAA	VMELISVLYPPLRSRLTGVT*	-1.256	0.003173	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26169	PTLPCNSRYMIDFAYAGYDD			20	SLAY-screened peptide P4519			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACGCTCCCTTGTAATTCGAGGTACATGATCGATTTCGCGTATGCTGGTTATGATGACTAA	PTLPCNSRYMIDFAYAGYDD*	-1.256	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26170	CP			2	SLAY-screened peptide P4520			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCTTAGCGCATCACGGACTGCTTCATCATCTTCTCGCATCTGAGTGAGATGCTGAAGTAA	CP*RITDCFIIFSHLSEMLK*	-1.255	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26171	LVYSADAMFYPTYTLSGTRR			20	SLAY-screened peptide P4521			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGTCTACAGCGCTGACGCGATGTTCTACCCCACTTATACCCTGTCGGGCACCAGGCGCTAA	LVYSADAMFYPTYTLSGTRR*	-1.255	3.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26172	MFHPCFTSAPDGSYLRRYVK			20	SLAY-screened peptide P4522			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTTCCATCCGTGTTTTACGTCTGCCCCCGATGGCTCGTATTTGCGCAGGTACGTTAAGTAA	MFHPCFTSAPDGSYLRRYVK*	-1.255	0.018897	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26173	VLWNLCPDLQGTEYVS			16	SLAY-screened peptide P4523			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCTCTGGAACCTTTGTCCTGACCTGCAGGGCACCGAGTACGTTAGTTAGTTCGGCAAGTAA	VLWNLCPDLQGTEYVS*FGK*	-1.254	0.018324	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26174	TSCYPYSYTSSGWDSLVIGL			20	SLAY-screened peptide P4524			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTCGTGCTACCCCTATAGCTACACGTCTTCCGGCTGGGACTCCTTGGTGATCGGTCTTTAA	TSCYPYSYTSSGWDSLVIGL*	-1.254	0.001416	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26175	HTTLFAATPGPMAAFPIIYLT			21	SLAY-screened peptide P4525			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACTACATTATTCGCAGCGACGCCCGGTCCCATGGCAGCCTTTCCGATTATTTATTTAACT	HTTLFAATPGPMAAFPIIYLT	-1.254	0.0272	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26176	AAFPIPTSRPRNFGRARLLL			20	SLAY-screened peptide P4526			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGCTTTCCCTATTCCCACTAGCCGGCCGCGTAATTTTGGCCGTGCCCGCCTCCTGCTGTAA	AAFPIPTSRPRNFGRARLLL*	-1.254	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26177	CINYIFYNTIRSDYVTHLVS			20	SLAY-screened peptide P4527			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCATCAATTACATCTTTTACAATACCATCCGTAGCGACTATGTGACCCACTTGGTGAGCTAA	CINYIFYNTIRSDYVTHLVS*	-1.254	0.007791	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26178	CFRARHSLDLKNIPLRLV			18	SLAY-screened peptide P4528			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTTTAGGGCGCGCCATAGTCTTGACCTTAAGAATATCCCTCTCAGGCTTGTTTAGTTCTAA	CFRARHSLDLKNIPLRLV*F*	-1.254	0.031395	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26179	WPWHTLYEALYLRYTTTSKS			20	SLAY-screened peptide P4529			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCCCTGGCATACTCTGTATGAGGCTCTTTACCTGCGTTACACCACTACTTCTAAGTCGTAA	WPWHTLYEALYLRYTTTSKS*	-1.254	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26180	PLQCNHTLHHPWTSVIYGDM			20	SLAY-screened peptide P4530			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTGCAGTGCAACCACACTCTTCATCATCCGTGGACTTCCGTTATCTATGGGGACATGTAA	PLQCNHTLHHPWTSVIYGDM*	-1.254	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26181	EHHHSTQFYTNQRDCTIGPR			20	SLAY-screened peptide P4531			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCACCATCATAGTACCCAGTTTTATACCAACCAGCGCGATTGCACTATTGGGCCCCGCTAA	EHHHSTQFYTNQRDCTIGPR*	-1.254	0.002361	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26182	TLFTYLAVFFHLILAGHFFH			20	SLAY-screened peptide P4532			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTTTTCACCTACCTTGCCGTCTTCTTCCATCTCATCCTTGCCGGCCACTTTTTCCACTAA	TLFTYLAVFFHLILAGHFFH*	-1.253	0.015288	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26183	HCPPVYWFLNSSSGLDTPSP			20	SLAY-screened peptide P4533			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGCCCCCCCGTGTATTGGTTCCTCAATTCCAGCTCCGGCCTGGACACTCCGTCCCCCTAA	HCPPVYWFLNSSSGLDTPSP*	-1.253	0.000398	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26184	TECELTNRCLLPHIYIVFKP			20	SLAY-screened peptide P4534			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGAGTGCGAGCTCACGAATAGGTGCCTTTTGCCTCACATCTATATCGTGTTTAAGCCCTAA	TECELTNRCLLPHIYIVFKP*	-1.253	0.000208	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26185	AHVPSRASAHEGA			13	SLAY-screened peptide P4535			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCATGTTCCGAGCCGGGCGTCTGCTCACGAGGGAGCCTAATCTCGACACTACTCGTGCCCG	AHVPSRASAHEGA*SRHYSCP	-1.253	0.008102	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26186	CSPTRRRPAYCPSEDQAL			18	SLAY-screened peptide P4536			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTCTCCAACACGCCGGCGTCCGGCGTACTGCCCTTCAGAGGACCAGGCCCTATGATTAACT	CSPTRRRPAYCPSEDQAL*LT	-1.253	0.018915	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26187	CMYTNVVHPYPCQPTYYRCP			20	SLAY-screened peptide P4537			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCATGTACACTAATGTCGTCCACCCTTACCCCTGCCAGCCCACTTATTATCGTTGCCCCTAA	CMYTNVVHPYPCQPTYYRCP*	-1.253	0.001549	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26188	TSQNSIVFSTSGRRKKIRRGN			21	SLAY-screened peptide P4538			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTCGCAGAATTCTATTGTTTTTAGCACCTCTGGCCGTCGGAAGAAAATACGACGTGGTAAC	TSQNSIVFSTSGRRKKIRRGN	-1.252	0.000323	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26189	YDPPSSSAIHSFLTPWFFLC			20	SLAY-screened peptide P4539			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGACCCTCCTAGCTCTTCGGCTATCCACTCTTTTTTGACCCCCTGGTTTTTCCTTTGCTAA	YDPPSSSAIHSFLTPWFFLC*	-1.252	0.039065	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26190	PVHLDMTSAHINSKRNTLTS			20	SLAY-screened peptide P4540			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGTCCATTTGGATATGACGAGTGCGCACATTAACAGTAAGAGGAATACGCTGACTTCTTAA	PVHLDMTSAHINSKRNTLTS*	-1.252	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26191	SVDRRHKFELLLEHR			15	SLAY-screened peptide P4541			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGTTGACCGCCGTCATAAGTTTGAGCTCCTATTAGAGCATCGCTGACTACGCTACCTAACT	SVDRRHKFELLLEHR*LRYLT	-1.252	0.001802	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26192	TTDSSPSHAIESQRLDNTHY			20	SLAY-screened peptide P4542			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACTGATAGTAGCCCGTCCCATGCCATCGAGTCGCAGAGGCTCGACAACACCCATTACTAA	TTDSSPSHAIESQRLDNTHY*	-1.252	0.031237	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26193	ATCWHPLRPSVFTS			14	SLAY-screened peptide P4543			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACCTGCTGGCATCCCCTCCGTCCTTCTGTGTTTACCTCCTAGGAGCGCCCCTCCTCTTAA	ATCWHPLRPSVFTS*ERPSS*	-1.252	0.021849	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26194	RTCHGSTSTHSPALFQFFHY			20	SLAY-screened peptide P4544			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTACTTGCCATGGTTCGACCAGTACCCACAGTCCTGCCCTCTTTCAGTTTTTCCATTATTAA	RTCHGSTSTHSPALFQFFHY*	-1.251	0.002751	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26195	TETPNSATDTAHTETYCSYI			20	SLAY-screened peptide P4545			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGAGACCCCTAACAGTGCGACCGACACCGCCCATACTGAGACTTATTGCAGTTACATCTAA	TETPNSATDTAHTETYCSYI*	-1.251	0.000768	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26196	TVCPLCCRPLRRRIPALFPSN			21	SLAY-screened peptide P4546			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTGTGCCCGCTGTGCTGCAGGCCGCTTCGCCGCCGTATCCCAGCCCTTTTTCCGTCTAAC	TVCPLCCRPLRRRIPALFPSN	-1.251	0.001752	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26197	IDRRYLGTVSFA			12	SLAY-screened peptide P4547			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGACCGCCGTTACCTGGGCACTGTTTCGTTTGCCTAGATTAGCACCTTCCCTGGTGTTTAA	IDRRYLGTVSFA*ISTFPGV*	-1.25	0.026431	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26198	CHPGLYKKLRLYYLAYTECD			20	SLAY-screened peptide P4548			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCACCCGGGCCTGTACAAGAAGTTGCGTCTGTATTATCTGGCTTATACTGAGTGTGATTAA	CHPGLYKKLRLYYLAYTECD*	-1.25	0.03364	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26199	TRCIPFLMLFVICPQRGTIPN			21	SLAY-screened peptide P4549			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCGGTGTATTCCCTTCCTTATGCTCTTCGTTATTTGCCCTCAACGCGGCACCATACCTAAC	TRCIPFLMLFVICPQRGTIPN	-1.25	0.000367	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26200	CLQIHPKLCLCEFYV			15	SLAY-screened peptide P4550			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTCCAGATTCATCCGAAGCTTTGCCTCTGCGAGTTCTACGTTTAGAATGATGAGTGCTAA	CLQIHPKLCLCEFYV*NDEC*	-1.25	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26201	PCWGYLVLPRYSDFTKPLY			19	SLAY-screened peptide P4551			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGCTGGGGGTACCTCGTCTTGCCTAGGTATTCTGATTTCACTAAGCCGCTTTACTAGTAA	PCWGYLVLPRYSDFTKPLY**	-1.25	0.000134	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26202	PMDSSNDGSYNEGHKSIDYV			20	SLAY-screened peptide P4552			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATGGACTCTAGTAACGACGGCTCTTACAACGAGGGCCACAAGAGCATTGACTATGTCTAA	PMDSSNDGSYNEGHKSIDYV*	-1.25	0.000537	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26203	CEELPQCTRHHFDPFSNSVP			20	SLAY-screened peptide P4553			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGAGGAGCTGCCGCAGTGCACCAGGCATCATTTCGACCCGTTTAGTAACTCCGTCCCCTAA	CEELPQCTRHHFDPFSNSVP*	-1.25	0.000761	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26204	PCRRSRSHSPSRTNEPHLPP			20	SLAY-screened peptide P4554			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGTCGGCGGTCTCGTTCGCACTCCCCCTCTCGCACTAACGAGCCGCATCTTCCTCCGTAA	PCRRSRSHSPSRTNEPHLPP*	-1.249	0.004256	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26205	TCKCSQSSQHTNRQFNSNYP			20	SLAY-screened peptide P4555			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTGTAAGTGCTCGCAGTCTTCCCAGCATACCAATCGTCAGTTTAATTCCAACTATCCGTAA	TCKCSQSSQHTNRQFNSNYP*	-1.249	0.005024	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26206	VDLHLFLLFLSTTFYLRLKA			20	SLAY-screened peptide P4556			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGACCTCCACCTGTTTTTGCTCTTTCTCTCTACCACTTTCTACCTCCGCCTTAAGGCTTAA	VDLHLFLLFLSTTFYLRLKA*	-1.249	0.007748	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26207	IVTRSNTSTPPPITCLIIGL			20	SLAY-screened peptide P4557			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGTTACGCGCAGCAATACCAGCACCCCCCCCCCGATCACCTGTTTGATTATCGGGCTGTAA	IVTRSNTSTPPPITCLIIGL*	-1.249	0.024194	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26208	VLCPNRSPASYSNMIKELHI			20	SLAY-screened peptide P4558			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCTGTGTCCCAACAGGTCCCCGGCCTCCTACAGTAACATGATTAAGGAGCTGCACATCTAA	VLCPNRSPASYSNMIKELHI*	-1.249	0.000813	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26209	NDHYVPIHPGYCDPFDTYRV			20	SLAY-screened peptide P4559			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGATCACTATGTTCCGATTCATCCGGGGTACTGCGATCCGTTTGACACTTACAGGGTGTAA	NDHYVPIHPGYCDPFDTYRV*	-1.249	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26210	FAPLCCDNWHLPPVYPSADP			20	SLAY-screened peptide P4560			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGCTCCTTTGTGTTGTGATAACTGGCATCTTCCCCCGGTCTACCCCAGCGCCGACCCTTAA	FAPLCCDNWHLPPVYPSADP*	-1.249	0.001378	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26211	PRYCNWPSFYAYRHIDTIYV			20	SLAY-screened peptide P4561			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGCTACTGCAATTGGCCCTCTTTCTACGCGTATCGCCACATTGACACGATTTATGTCTAA	PRYCNWPSFYAYRHIDTIYV*	-1.249	0.007994	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26212	LDSMCRFFRRAIRRLTVRPGN			21	SLAY-screened peptide P4562			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGGACAGCATGTGTAGGTTTTTCCGGCGAGCGATTCGTCGCTTAACTGTACGTCCGGGTAAC	LDSMCRFFRRAIRRLTVRPGN	-1.248	0.000349	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26213	NALMPHPDIVHCMHNYSTLT			20	SLAY-screened peptide P4563			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGCTCTGATGCCTCATCCCGATATTGTGCATTGCATGCACAATTATTCTACGCTGACTTAA	NALMPHPDIVHCMHNYSTLT*	-1.248	0.045243	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26214	YNGKRRTGLCILRDAFGVKQ			20	SLAY-screened peptide P4564			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACAATGGCAAGCGTCGCACGGGTCTGTGTATCCTCCGCGACGCGTTTGGGGTTAAGCAGTAA	YNGKRRTGLCILRDAFGVKQ*	-1.248	0.005226	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26215	PTNYNAVWLITSLT			14	SLAY-screened peptide P4565			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACCAATTATAACGCCGTTTGGCTGATTACGTCGCTCACCTAGTATGTTTATCAGAAGTAA	PTNYNAVWLITSLT*YVYQK*	-1.248	0.006167	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26216	PHIIMVISLTLRLRRTSGLCN			21	SLAY-screened peptide P4566			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCACATCATTATGGTGATCTCACTTACTTTACGCTTACGTCGAACATCCGGACTCTGTAAC	PHIIMVISLTLRLRRTSGLCN	-1.247	0.000423	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26217	RLLICAPAREHGSDMSN			17	SLAY-screened peptide P4567			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCTCCTCATTTGCGCCCCTGCGAGGGAGCACGGTAGCGATATGTCTAACTAGGCTTCTTAA	RLLICAPAREHGSDMSN*AS*	-1.247	0.00087	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26218	MCFCNLCLRPALPPICYQTM			20	SLAY-screened peptide P4568			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTGTTTTTGTAACCTCTGCCTTCGGCCCGCCCTGCCCCCGATTTGCTACCAGACGATGTAA	MCFCNLCLRPALPPICYQTM*	-1.247	0.037433	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26219	TPVPPSVPDHSSHECKTTDA			20	SLAY-screened peptide P4569			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCCCGTCCCTCCGTCCGTGCCCGATCATTCGAGTCATGAGTGCAAGACTACCGATGCGTAA	TPVPPSVPDHSSHECKTTDA*	-1.247	0.015126	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26220	LMSLPRIPKSVWLYASSSVP			20	SLAY-screened peptide P4570			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATGAGTCTTCCCCGTATTCCGAAGAGTGTTTGGCTCTATGCGAGCAGTTCCGTCCCTTAA	LMSLPRIPKSVWLYASSSVP*	-1.247	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26221	VSPGTSVYDVHMYIYYRTTN			20	SLAY-screened peptide P4571			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTCGCCTGGCACCTCGGTCTATGACGTCCACATGTATATTTACTACCGGACTACGAATTAA	VSPGTSVYDVHMYIYYRTTN*	-1.247	0.032438	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26222	QAADSLPNSCPITLRGPSTH			20	SLAY-screened peptide P4572			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGCCGCTGACTCCTTGCCTAACAGCTGCCCTATTACCCTCCGTGGGCCTTCCACCCACTAA	QAADSLPNSCPITLRGPSTH*	-1.247	0.021216	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26223	PDSALTDSSIWNPARWKPFI			20	SLAY-screened peptide P4573			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGATTCTGCGCTTACCGATTCCTCTATCTGGAATCCCGCTCGTTGGAAGCCTTTCATTTAA	PDSALTDSSIWNPARWKPFI*	-1.247	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26224	PAVFPTTSCAYCPVHTALTI			20	SLAY-screened peptide P4574			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCTGTGTTTCCCACCACTTCTTGCGCTTATTGTCCGGTTCACACGGCGCTTACTATCTAA	PAVFPTTSCAYCPVHTALTI*	-1.247	0.027616	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26225	RGLILCTPAIVLSLTAASNL			20	SLAY-screened peptide P4575			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGGCCTCATCCTGTGCACGCCCGCGATCGTCTTGAGCCTTACGGCCGCTTCTAATTTGTAA	RGLILCTPAIVLSLTAASNL*	-1.246	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26226	GFLYSPTNSPFLISTP			16	SLAY-screened peptide P4576			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTTCTTGTATTCGCCTACGAATAGTCCTTTTCTCATCTCTACTCCCTAGCGTAGCAAGTAA	GFLYSPTNSPFLISTP*RSK*	-1.246	0.001922	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26227	QDHSTFCLSITLDLSFRFGP			20	SLAY-screened peptide P4577			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGATCATTCGACCTTCTGTTTGTCCATCACTCTTGACCTTTCTTTCCGGTTCGGTCCCTAA	QDHSTFCLSITLDLSFRFGP*	-1.246	0.000321	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26228	PPRSLRDDLSHCDVPAYYFI			20	SLAY-screened peptide P4578			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCCCGGAGCCTTCGCGATGACCTTTCTCATTGTGACGTCCCCGCCTATTACTTTATTTAA	PPRSLRDDLSHCDVPAYYFI*	-1.246	0.001951	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26229	LPCGCFNSTYGSNPTSIVLL			20	SLAY-screened peptide P4579			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCGTGCGGCTGTTTCAATTCGACTTATGGCAGCAACCCTACTAGCATCGTTTTGCTCTAA	LPCGCFNSTYGSNPTSIVLL*	-1.245	0.033909	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26230	RSIYRNAAALNHVNNTLRNI			20	SLAY-screened peptide P4580			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCTATTTATCGCAATGCTGCTGCCCTCAATCATGTCAACAACACCTTGCGTAACATTTAA	RSIYRNAAALNHVNNTLRNI*	-1.245	0.042825	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26231	SGRLLHRLSTDLTDDYYDCM			20	SLAY-screened peptide P4581			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGGCCGGCTCTTGCATCGGCTTTCGACCGACCTGACTGATGACTACTATGATTGCATGTAA	SGRLLHRLSTDLTDDYYDCM*	-1.245	0.000273	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26232	KTSYDTNYFFCYL			13	SLAY-screened peptide P4582			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGACCTCTTATGACACCAATTATTTTTTCTGTTATTTGTAGTATCATAGACTAACCAGTAAC	KTSYDTNYFFCYL*YHRLTSN	-1.244	0.006572	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26233	SQYFCASASPVSVDLPIPSR			20	SLAY-screened peptide P4583			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCAGTACTTTTGCGCCTCTGCTTCGCCGGTTTCTGTGGACCTTCCTATTCCGTCCCGGTAA	SQYFCASASPVSVDLPIPSR*	-1.244	6.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26234	QSRYCACNSMCSYNTMGILL			20	SLAY-screened peptide P4584			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTCCCGGTACTGTGCGTGTAACTCCATGTGCTCCTACAATACGATGGGTATCCTCCTGTAA	QSRYCACNSMCSYNTMGILL*	-1.244	0.010379	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26235	CDPDPYSAMFLLATSAHTPD			20	SLAY-screened peptide P4585			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGACCCCGACCCTTACAGTGCGATGTTCCTGCTCGCCACGTCTGCGCATACTCCCGATTAA	CDPDPYSAMFLLATSAHTPD*	-1.244	0.042762	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26236	ALQETSRYGLPSLNCLTLIA			20	SLAY-screened peptide P4586			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCTGCAGGAGACCTCCCGCTATGGCCTCCCGAGCCTCAACTGCCTTACCCTGATTGCTTAA	ALQETSRYGLPSLNCLTLIA*	-1.244	0.028082	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26237	PPTILT			6	SLAY-screened peptide P4587			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTACCATTTTGACCTAGATTCTGATGTCCTCTATGATTACTAAATGCTTTATAACTAAC	PPTILT*ILMSSMITKCFITN	-1.244	0.019845	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26238	DAQSLLSHPAHHLKEPSPDS			20	SLAY-screened peptide P4588			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGCCCAGAGCCTGCTCTCGCATCCTGCGCACCACCTTAAGGAGCCCAGCCCCGACTCCTAA	DAQSLLSHPAHHLKEPSPDS*	-1.243	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26239	TLVCPHPTSYPHTSSCSNCT			20	SLAY-screened peptide P4589			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCTGGTGTGCCCCCATCCGACCTCTTATCCGCATACTTCCTCGTGTTCCAACTGCACCTAA	TLVCPHPTSYPHTSSCSNCT*	-1.243	0.003229	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26240	IHSGCHYKLPCCNFCWQCSL			20	SLAY-screened peptide P4590			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCATTCCGGGTGCCATTACAAGTTGCCCTGCTGCAACTTCTGCTGGCAGTGTTCCCTCTAA	IHSGCHYKLPCCNFCWQCSL*	-1.243	0.040231	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26241	MITSSPYCYCPIFYNYLSTN			20	SLAY-screened peptide P4591			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGATTACGAGCAGTCCCTACTGTTATTGCCCTATCTTTTACAATTATCTCTCTACTAATTAA	MITSSPYCYCPIFYNYLSTN*	-1.243	1.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26242	ICRSRYGLGFTSSNWDNTTP			20	SLAY-screened peptide P4592			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTTGTCGGTCTCGCTACGGCCTTGGCTTCACCTCTTCCAATTGGGATAATACGACTCCGTAA	ICRSRYGLGFTSSNWDNTTP*	-1.243	0.026415	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26243	PCCSHTTCVTGLYIA			15	SLAY-screened peptide P4593			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGTTGCTCCCATACTACCTGCGTTACTGGGCTCTACATTGCCTAGAATAATTAGAACTAA	PCCSHTTCVTGLYIA*NN*N*	-1.243	0.00104	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26244	CLYTKQATFDDPPALDFQVI			20	SLAY-screened peptide P4594			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCTGTATACTAAGCAGGCCACCTTCGACGACCCTCCCGCGTTGGATTTCCAGGTGATCTAA	CLYTKQATFDDPPALDFQVI*	-1.243	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26245	HSHRSATRLTPCHGITQRLDF			21	SLAY-screened peptide P4595			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTCTCACCGTTCCGCGACTCGTTTGACCCCGTGTCATGGTATCACTCAGCGCCTCGACTTC	HSHRSATRLTPCHGITQRLDF	-1.242	0.027328	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26246	HTGTDILAPCPPSIYEVLVI			20	SLAY-screened peptide P4596			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACTGGTACGGACATCCTTGCCCCGTGTCCGCCGTCCATCTATGAGGTCCTTGTCATCTAA	HTGTDILAPCPPSIYEVLVI*	-1.242	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26247	ALVWHHCFLPFLIMRKWHMC			20	SLAY-screened peptide P4597			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCTCGTGTGGCACCATTGCTTCCTTCCTTTCCTCATTATGCGCAAGTGGCACATGTGCTAA	ALVWHHCFLPFLIMRKWHMC*	-1.242	0.043768	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26248	LHGPRRRFKWRRRYDGSSPT			20	SLAY-screened peptide P4598			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCACGGGCCGCGCCGTCGGTTCAAGTGGCGGCGCCGTTACGATGGCTCGAGTCCCACCTAA	LHGPRRRFKWRRRYDGSSPT*	-1.242	0.04457	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26249	DYTAPYSDPRCYRPFRLLYH			20	SLAY-screened peptide P4599			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTACACCGCGCCCTATAGCGACCCCCGGTGCTATAGGCCTTTCCGTCTCCTGTACCATTAA	DYTAPYSDPRCYRPFRLLYH*	-1.242	3.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26250	HDRGDPLMPYSTNLTTFSTT			20	SLAY-screened peptide P4600			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGACCGGGGCGACCCTCTTATGCCGTACTCTACTAACCTTACGACCTTTAGCACGACCTAA	HDRGDPLMPYSTNLTTFSTT*	-1.241	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26251	RPSAHYNDLQMSVEDSRSDH			20	SLAY-screened peptide P4601			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCTAGTGCTCATTATAACGATCTGCAGATGTCTGTTGAGGACTCCCGTTCTGATCATTAA	RPSAHYNDLQMSVEDSRSDH*	-1.241	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26252	CFDGCYRTTSSCPVPRFFTF			20	SLAY-screened peptide P4602			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTTTGACGGCTGCTACCGCACTACCTCTTCCTGCCCGGTTCCCAGGTTCTTTACTTTTTAA	CFDGCYRTTSSCPVPRFFTF*	-1.241	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26253	PDWYHDWPTYKLCTISCAGD			20	SLAY-screened peptide P4603			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGACTGGTACCACGACTGGCCTACCTATAAGCTTTGTACTATCAGCTGTGCGGGTGATTAA	PDWYHDWPTYKLCTISCAGD*	-1.241	0.001813	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26254	SNIARGIINY			10	SLAY-screened peptide P4604			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTAACATTGCCCGTGGGATCATTAACTATTAGTGGCAGGTTTTGACGGTCTTCTTCTTTTAA	SNIARGIINY*WQVLTVFFF*	-1.241	0.001417	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26255	RTCNRAPWRTLCIISRALFY			20	SLAY-screened peptide P4605			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACCTGTAACCGGGCTCCTTGGCGCACGTTGTGCATCATTTCGAGGGCCCTCTTTTATTAA	RTCNRAPWRTLCIISRALFY*	-1.24	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26256	AADVYMLHYFRYFPPSVSEV			20	SLAY-screened peptide P4606			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGCTGACGTCTACATGCTCCACTACTTTCGGTATTTTCCTCCCAGTGTCTCTGAGGTGTAA	AADVYMLHYFRYFPPSVSEV*	-1.24	0.001558	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26257	IIHFFITLYLASHYAPSIES			20	SLAY-screened peptide P4607			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTATTCACTTCTTTATTACCCTTTATTTGGCCAGCCATTATGCCCCTTCTATCGAGTCGTAA	IIHFFITLYLASHYAPSIES*	-1.24	0.025872	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26258	WYLSTGIRYDRNHTDWHFSM			20	SLAY-screened peptide P4608			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTATCTCAGCACTGGGATTCGCTACGACCGCAACCACACGGATTGGCATTTCAGCATGTAA	WYLSTGIRYDRNHTDWHFSM*	-1.24	0.01595	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26259	WTVTVDFRPRLKEDSRYYDS			20	SLAY-screened peptide P4609			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGACCGTTACCGTGGATTTCCGGCCCCGCCTTAAGGAGGACTCTCGCTACTATGACAGTTAA	WTVTVDFRPRLKEDSRYYDS*	-1.24	0.049093	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26260	APYCTPFSYFYYGPTLTYLP			20	SLAY-screened peptide P4610			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCCCTATTGTACCCCCTTTTCCTACTTCTACTATGGGCCGACTCTGACGTATTTGCCTTAA	APYCTPFSYFYYGPTLTYLP*	-1.24	0.000467	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26261	TDAVRVFSFTSD			12	SLAY-screened peptide P4611			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGACGCCGTTAGGGTGTTCAGTTTTACCTCTGATTAGAGCTGTCTCACCTAGTATCAGTAA	TDAVRVFSFTSD*SCLT*YQ*	-1.24	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26262	SARPSANTTPAIIVSCYELV			20	SLAY-screened peptide P4612			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGCCCGTCCCTCCGCCAATACTACGCCCGCTATCATCGTCTCGTGCTACGAGCTCGTTTAA	SARPSANTTPAIIVSCYELV*	-1.239	0.000162	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26263	SCRPSRSLFPYYNIVMVNRF			20	SLAY-screened peptide P4613			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTGCCGCCCCTCGCGGTCTCTCTTCCCTTATTACAACATTGTTATGGTCAATAGGTTCTAA	SCRPSRSLFPYYNIVMVNRF*	-1.239	0.000615	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26264	CHRFKVRNCNHAGIVVLTYI			20	SLAY-screened peptide P4614			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCACAGGTTTAAGGTCCGTAACTGCAACCACGCCGGTATCGTGGTCCTCACGTATATTTAA	CHRFKVRNCNHAGIVVLTYI*	-1.239	3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26265	ALCYRQMPMSSSFSNNVHSD			20	SLAY-screened peptide P4615			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCTCTGCTATAGGCAGATGCCCATGTCCAGCTCTTTCTCGAACAACGTTCACTCCGACTAA	ALCYRQMPMSSSFSNNVHSD*	-1.239	0.009023	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26266	LSAVDCHLHSIYGAFPLAVW			20	SLAY-screened peptide P4616			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCCGCCGTTGACTGCCATCTTCATAGCATCTACGGGGCCTTCCCGCTGGCCGTGTGGTAA	LSAVDCHLHSIYGAFPLAVW*	-1.239	0.001591	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26267	ANGNRSMYGIIHAISCVWLS			20	SLAY-screened peptide P4617			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAACGGGAACCGTAGCATGTACGGTATCATTCACGCTATTTCTTGTGTTTGGCTGTCCTAA	ANGNRSMYGIIHAISCVWLS*	-1.239	0.022594	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26268	RPLNPPYCGCPVLLPWAEIT			20	SLAY-screened peptide P4618			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCGCTGAACCCCCCTTACTGCGGGTGCCCTGTGCTGCTGCCCTGGGCGGAGATCACCTAA	RPLNPPYCGCPVLLPWAEIT*	-1.239	0.000588	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26269	NQPTSQRHECIDSAALPIIC			20	SLAY-screened peptide P4619			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCAGCCCACTAGTCAGCGTCATGAGTGCATTGACAGTGCCGCGCTCCCCATTATCTGCTAA	NQPTSQRHECIDSAALPIIC*	-1.239	0.000401	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26270	ASSGFFDRYTYCQFPMWQYL			20	SLAY-screened peptide P4620			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTCCTCCGGGTTTTTCGACCGCTACACGTACTGCCAGTTTCCCATGTGGCAGTACCTTTAA	ASSGFFDRYTYCQFPMWQYL*	-1.238	0.015255	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26271	RPLARSQNSHHG			12	SLAY-screened peptide P4621			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCTCTCGCTAGGTCTCAGAATTCCCATCATGGCTGACTAACACGTGCCGTCGCTCTTAAC	RPLARSQNSHHG*LTRAVALN	-1.238	0.001717	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26272	MALRHDYANNFYVVNVWNLY			20	SLAY-screened peptide P4622			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGCCCTCCGGCACGACTACGCTAACAACTTTTATGTTGTGAATGTGTGGAACCTTTACTAA	MALRHDYANNFYVVNVWNLY*	-1.237	6.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26273	PYWCPRNRLWPPSYLSHINS			20	SLAY-screened peptide P4623			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTATTGGTGTCCGAGGAATCGCTTGTGGCCGCCTAGTTATTTGAGTCACATCAATTCCTAA	PYWCPRNRLWPPSYLSHINS*	-1.237	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26274	HVSSELLHRSDKHSTRPYP			19	SLAY-screened peptide P4624			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGTGAGCTCGGAGCTTCTGCACCGGAGTGATAAGCATAGTACCCGCCCTTACCCCTAGTAA	HVSSELLHRSDKHSTRPYP**	-1.237	0.004625	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26275	SHIISHAVRRPRYMDSRKFL			20	SLAY-screened peptide P4625			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCACATTATTTCGCATGCGGTCCGCCGCCCGCGTTATATGGATAGCCGCAAGTTCCTGTAA	SHIISHAVRRPRYMDSRKFL*	-1.237	0.038155	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26276	HHLRAKHDYIF			11	SLAY-screened peptide P4626			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCATCTCCGCGCGAAGCATGATTATATTTTTTAGGCGTCCTTGCTCCCTAACAATAGGTAA	HHLRAKHDYIF*ASLLPNNR*	-1.236	3.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26277	LIPYFIHHLYRTLVTQIPTR			20	SLAY-screened peptide P4627			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCATCCCCTATTTTATCCATCACCTCTACAGGACTCTCGTTACCCAGATTCCCACCCGTTAA	LIPYFIHHLYRTLVTQIPTR*	-1.236	0.002528	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26278	PRAPDAMRRTFTMSFITLILN			21	SLAY-screened peptide P4628			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGTGCCCCCGATGCTATGCGCCGCACCTTTACAATGTCTTTCATAACGCTGATACTTAAC	PRAPDAMRRTFTMSFITLILN	-1.236	0.024888	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26279	TEDAHLYATACPHAPSVNRH			20	SLAY-screened peptide P4629			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGAGGATGCCCATCTCTACGCTACTGCGTGCCCCCACGCTCCGTCGGTTAACAGGCATTAA	TEDAHLYATACPHAPSVNRH*	-1.236	0.001759	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26280	GSVACTCRMRGHCHYSTVRI			20	SLAY-screened peptide P4630			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTCCGTCGCTTGCACGTGCCGGATGCGCGGCCACTGTCATTATTCCACGGTTCGTATCTAA	GSVACTCRMRGHCHYSTVRI*	-1.236	2.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26281	LPVHLGNNKPFSAWNNHSAV			20	SLAY-screened peptide P4631			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCGGTCCATCTTGGGAATAATAAGCCTTTTTCTGCCTGGAATAACCATTCTGCGGTTTAA	LPVHLGNNKPFSAWNNHSAV*	-1.236	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26282	ERHRAAFLSGTFHPVNSSNH			20	SLAY-screened peptide P4632			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCGCCACCGCGCCGCCTTTCTTTCTGGCACGTTTCATCCTGTCAATTCTAGCAACCATTAA	ERHRAAFLSGTFHPVNSSNH*	-1.236	0.000512	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26283	PTGQCTYVAPWYGYQSLWNK			20	SLAY-screened peptide P4633			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACCGGTCAGTGCACTTATGTTGCCCCCTGGTACGGTTATCAGAGCCTCTGGAACAAGTAA	PTGQCTYVAPWYGYQSLWNK*	-1.235	0.000182	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26284	LLLYFWIGRMSIDSSNCVFQ			20	SLAY-screened peptide P4634			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCTGCTTTACTTCTGGATTGGTCGTATGTCTATTGATAGTTCGAACTGTGTGTTCCAGTAA	LLLYFWIGRMSIDSSNCVFQ*	-1.235	0.011822	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26285	TPFRVFWLLGLIGIRIPRFLN			21	SLAY-screened peptide P4635			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCGTTTCGGGTCTTCTGGTTACTCGGCTTAATTGGAATACGCATACCTAGGTTTCTTAAC	TPFRVFWLLGLIGIRIPRFLN	-1.235	6.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26286	AFRTPYNMFDILQVRCIRPP			20	SLAY-screened peptide P4636			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTTTCGCACTCCTTACAACATGTTTGACATTCTCCAGGTGCGTTGCATCAGGCCCCCTTAA	AFRTPYNMFDILQVRCIRPP*	-1.235	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26287	LYPRLVRVHQHRCFPYFDLTE			21	SLAY-screened peptide P4637			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTACCCCCGATTGGTCCGAGTTCATCAGCACCGCTGTTTCCCTTATTTTGACTTAACTGAG	LYPRLVRVHQHRCFPYFDLTE	-1.235	0.000106	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26288	QFNNR			5	SLAY-screened peptide P4638			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTTTAACAATCGTTAGGCTCTCTATACGGCTACCAACATTGTCCGTTTTTTGGGTAAGTAA	QFNNR*ALYTATNIVRFLGK*	-1.234	0.008637	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26289	VLPTRDKPDVLPCFTATLIV			20	SLAY-screened peptide P4639			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCTTCCGACTCGGGATAAGCCGGATGTGCTTCCGTGCTTCACTGCCACCTTGATTGTCTAA	VLPTRDKPDVLPCFTATLIV*	-1.234	0.000786	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26290	HDLARHSHTSYDPADSINLR			20	SLAY-screened peptide P4640			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGATTTGGCCCGGCATAGCCATACGAGTTATGACCCCGCCGACTCCATCAATTTGAGGTAA	HDLARHSHTSYDPADSINLR*	-1.234	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26291	LSSFGQACKCVTL			13	SLAY-screened peptide P4641			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTCCAGTTTTGGGCAGGCGTGCAAGTGTGTTACCCTGTAGCGCTCCGAGAGTCTCTCGTAA	LSSFGQACKCVTL*RSESLS*	-1.234	0.000597	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26292	CRSMSRPTPPDSWLDNSRVR			20	SLAY-screened peptide P4642			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAGGTCTATGAGTAGGCCCACCCCCCCTGATAGTTGGCTCGACAACTCCAGGGTTCGCTAA	CRSMSRPTPPDSWLDNSRVR*	-1.234	0.037597	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26293	FRNIQDDFIHYNSINRVVIW			20	SLAY-screened peptide P4643			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCGTAACATTCAGGATGACTTCATCCACTACAATTCGATCAATCGCGTCGTTATCTGGTAA	FRNIQDDFIHYNSINRVVIW*	-1.234	0.000696	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26294	HPFDEKSHHCSDSYLSVSRF			20	SLAY-screened peptide P4644			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCGTTTGACGAGAAGAGCCACCACTGTAGCGATAGTTACCTTTCCGTGAGCCGGTTCTAA	HPFDEKSHHCSDSYLSVSRF*	-1.234	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26295	MGSPIGWTVIPTVFT			15	SLAY-screened peptide P4645			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGGTTCTCCCATCGGTTGGACTGTAATACCCACAGTGTTCACGTGAAGACGAAGGTCTAAC	MGSPIGWTVIPTVFT*RRRSN	-1.233	0.000633	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26296	LDEHYSQGHNSKRFNLFLTN			20	SLAY-screened peptide P4646			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGATGAGCATTACTCTCAGGGCCATAATTCTAAGCGCTTTAACTTGTTCTTGACGAACTAA	LDEHYSQGHNSKRFNLFLTN*	-1.233	0.02077	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26297	IHRRISTVHRDP			12	SLAY-screened peptide P4647			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCATCGGCGTATCTCTACGGTTCACCGTGATCCCTAGAATGATTCGTAGCTTCGCCATTAA	IHRRISTVHRDP*NDS*LRH*	-1.233	1.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26298	PPKGRRICYLHVPHDLPTSTN			21	SLAY-screened peptide P4648			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCGAAGGGGCGGCGCATTTGTTATCTCCACGTTCCGCATGACCTACCTACTTCAACTAAC	PPKGRRICYLHVPHDLPTSTN	-1.233	0.001654	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26299	PTDDPDSSNNMCRRLHIGLV			20	SLAY-screened peptide P4649			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACCGACGATCCCGACAGCAGCAACAACATGTGTCGTCGCCTGCACATCGGTCTCGTTTAA	PTDDPDSSNNMCRRLHIGLV*	-1.233	0.000441	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26300	HTLVPHTCHCIPPHGFMIYI			20	SLAY-screened peptide P4650			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACCCTTGTCCCCCATACGTGCCACTGTATCCCTCCTCACGGTTTTATGATTTATATTTAA	HTLVPHTCHCIPPHGFMIYI*	-1.233	0.000401	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26301	LTVPLY			6	SLAY-screened peptide P4651			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGACTGTTCCTCTCTACTAGACTTCTGTCTCTAATCGCTAGAAGTATCCGGTGCGCAAGTAA	LTVPLY*TSVSNR*KYPVRK*	-1.232	0.000691	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26302	RFHINTACQFPEPTNIP			17	SLAY-screened peptide P4652			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTTCCACATTAATACCGCCTGCCAGTTCCCTGAGCCGACGAATATCCCTTAGAATTAGTAA	RFHINTACQFPEPTNIP*N**	-1.232	0.000713	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26303	ATRVHTTGPMNDAFLQCRYL			20	SLAY-screened peptide P4653			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACCCGTGTGCATACCACCGGTCCTATGAACGATGCTTTTCTGCAGTGCCGTTACCTCTAA	ATRVHTTGPMNDAFLQCRYL*	-1.232	0.014504	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26304	AGGIALTTHRWASYSLPGNK			20	SLAY-screened peptide P4654			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGGCGGGATCGCCCTGACCACCCATAGGTGGGCCTCCTATTCTCTGCCCGGTAACAAGTAA	AGGIALTTHRWASYSLPGNK*	-1.232	0.003362	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26305	AITNDGIRNNTLKLKCRWAG			20	SLAY-screened peptide P4655			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGATTACTAATGACGGCATTCGTAACAATACGCTTAAGCTGAAGTGCCGCTGGGCTGGCTAA	AITNDGIRNNTLKLKCRWAG*	-1.232	0.019702	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26306	ALAATRSPQRPSFPDSCGDR			20	SLAY-screened peptide P4656			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCTCGCCGCCACTCGGAGCCCCCAGCGTCCCTCCTTCCCTGATAGCTGCGGTGACCGTTAA	ALAATRSPQRPSFPDSCGDR*	-1.232	0.002014	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26307	SSMFHCLLTACCYTLGMTPI			20	SLAY-screened peptide P4657			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTCCATGTTTCATTGTCTCCTTACTGCTTGCTGTTACACCCTGGGTATGACTCCTATCTAA	SSMFHCLLTACCYTLGMTPI*	-1.231	0.030548	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26308	LCPPARLHRPGESQPPLLLL			20	SLAY-screened peptide P4658			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTGCCCGCCTGCGCGCCTCCACCGTCCTGGGGAGTCTCAGCCTCCCCTTTTGTTGCTCTAA	LCPPARLHRPGESQPPLLLL*	-1.231	0.001447	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26309	CRPSIGYTDQPFVDLDQFII			20	SLAY-screened peptide P4659			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAGGCCTTCCATTGGTTATACTGACCAGCCTTTTGTTGATCTTGATCAGTTTATCATTTAA	CRPSIGYTDQPFVDLDQFII*	-1.231	0.001002	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26310	EDRKHTNHSSYCIPLDIVDS			20	SLAY-screened peptide P4660			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGATCGTAAGCACACCAACCACTCCTCCTATTGCATCCCCCTCGACATCGTTGATTCTTAA	EDRKHTNHSSYCIPLDIVDS*	-1.231	0.007854	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26311	NRPTTALMCPYWI			13	SLAY-screened peptide P4661			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCGCCCCACTACCGCTCTCATGTGTCCTTACTGGATTTAGCTTCACTACAGGCGCGCGTAA	NRPTTALMCPYWI*LHYRRA*	-1.231	0.000167	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26312	FFHYYPFRVTPCTISSIYYS			20	SLAY-screened peptide P4662			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTTTCACTACTACCCTTTTCGCGTCACTCCCTGCACTATTTCTAGCATTTATTATAGCTAA	FFHYYPFRVTPCTISSIYYS*	-1.231	0.000486	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26313	PTYYVRCAYNRDPPFLSATA			20	SLAY-screened peptide P4663			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACCTACTACGTTCGGTGTGCGTATAACCGTGATCCTCCCTTCTTGTCCGCGACCGCGTAA	PTYYVRCAYNRDPPFLSATA*	-1.23	0.026039	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26314	SASFLCTAIYDRL			13	SLAY-screened peptide P4664			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGCTTCTTTCCTGTGCACCGCTATTTACGATCGGTTGTAGTATGTTTATACTGTGACTTAA	SASFLCTAIYDRL*YVYTVT*	-1.23	0.003282	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26315	VPMATCTPPRGSIQSVDGES			20	SLAY-screened peptide P4665			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCCCATGGCCACTTGCACGCCCCCTCGGGGTTCTATTCAGTCCGTCGACGGGGAGTCCTAA	VPMATCTPPRGSIQSVDGES*	-1.23	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26316	PFHEHDATRP			10	SLAY-screened peptide P4666			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTTCACGAGCACGATGCCACTAGGCCTTAGCGGAGCACTAACTGTAATCGGACCGCGTAA	PFHEHDATRP*RSTNCNRTA*	-1.23	0.00163	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26317	PRRRFDPSCYQLYSLTMYDN			20	SLAY-screened peptide P4667			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGTCGCCGTTTCGACCCGTCCTGTTATCAGCTGTACTCTCTCACTATGTATGATAACTAA	PRRRFDPSCYQLYSLTMYDN*	-1.23	0.046243	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26318	GAHSCPAIITLPHRLAIV			18	SLAY-screened peptide P4668			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGGCGCACTCCTGTCCGGCCATTATCACCCTCCCTCACCGCCTGGCGATTGTTTAGTACTAA	GAHSCPAIITLPHRLAIV*Y*	-1.23	0.003652	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26319	RSAAFAIGSTDGTLTSNAYS			20	SLAY-screened peptide P4669			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAGCGCCGCCTTCGCCATCGGCTCTACTGACGGCACGCTCACTAGCAATGCCTACTCCTAA	RSAAFAIGSTDGTLTSNAYS*	-1.229	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26320	DIPVSSIN			8	SLAY-screened peptide P4670			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATATCCCCGTTTCTAGCATCAACTAGAGGCACTATTCTATGCTCGCCCTGTAGAACCACTAA	DIPVSSIN*RHYSMLAL*NH*	-1.229	0.000679	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26321	PGCTPLVMTSFNPWYAMTCC			20	SLAY-screened peptide P4671			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGTTGCACCCCGTTGGTCATGACTAGCTTTAACCCCTGGTACGCCATGACCTGTTGTTAA	PGCTPLVMTSFNPWYAMTCC*	-1.228	0.003504	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26322	SLLRFVCALARRFASDYYRG			20	SLAY-screened peptide P4672			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCTCCTGCGCTTTGTTTGCGCGCTCGCGCGGCGTTTCGCCTCCGACTACTATAGGGGCTAA	SLLRFVCALARRFASDYYRG*	-1.228	0.009341	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26323	GLSNTCEGYFPWPYNRLFYY			20	SLAY-screened peptide P4673			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCTTAGTAACACTTGTGAGGGTTACTTTCCTTGGCCGTACAATCGCTTGTTCTACTATTAA	GLSNTCEGYFPWPYNRLFYY*	-1.228	0.004436	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26324	LYEI			4	SLAY-screened peptide P4674			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTATGAGATTTAGGTGTAGCGTACCTTTGACAGTAAGAGCACTAATAATTACTTGAACTAA	LYEI*V*RTFDSKSTNNYLN*	-1.228	0.011154	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26325	CLLVPHAWLLY			11	SLAY-screened peptide P4675			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCTCCTGGTCCCTCACGCGTGGTTGCTTTATTAGAAGCCCGGCATTTTTTAGTGGTTTTAA	CLLVPHAWLLY*KPGIF*WF*	-1.228	0.002396	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26326	RPPYDYQHYWRHCHNLCEQF			20	SLAY-screened peptide P4676			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCCCCTTATGACTACCAGCACTATTGGCGGCACTGCCATAATCTTTGTGAGCAGTTCTAA	RPPYDYQHYWRHCHNLCEQF*	-1.227	0.030873	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26327	PSRSLKCMWSATHDSTDNFV			20	SLAY-screened peptide P4677			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTCGCGGAGCCTTAAGTGTATGTGGAGCGCCACGCACGATTCCACTGATAACTTCGTTTAA	PSRSLKCMWSATHDSTDNFV*	-1.227	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26328	LAYTLVDCKTFSIRASPKRP			20	SLAY-screened peptide P4678			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGCCTACACGCTGGTTGACTGTAAGACTTTTTCCATCCGGGCTTCTCCCAAGAGGCCGTAA	LAYTLVDCKTFSIRASPKRP*	-1.227	0.008722	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26329	ILGEWIHNFLHTRQHNRMAD			20	SLAY-screened peptide P4679			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCTTGGCGAGTGGATCCATAATTTCCTGCATACTCGCCAGCATAATCGGATGGCTGACTAA	ILGEWIHNFLHTRQHNRMAD*	-1.227	0.018601	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26330	VNPSVCLPSGVYTILPLVTE			20	SLAY-screened peptide P4680			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTAACCCGTCTGTCTGCCTCCCCAGCGGTGTCTATACGATTCTCCCGCTTGTCACCGAGTAA	VNPSVCLPSGVYTILPLVTE*	-1.227	0.037849	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26331	PHGADLLPNNAPVNVCVFHSN			21	SLAY-screened peptide P4681			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCATGGCGCTGATCTGCTCCCTAACAACGCTCCCGTCAATGTCTGCGTTTTCCATAGTAAC	PHGADLLPNNAPVNVCVFHSN	-1.227	0.015962	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26332	PRARNLLPEPYRCGAFFIPL			20	SLAY-screened peptide P4682			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGGGCCCGCAACCTTCTTCCCGAGCCCTACCGCTGTGGGGCCTTTTTTATTCCTCTGTAA	PRARNLLPEPYRCGAFFIPL*	-1.226	1.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26333	IPYGPGYVLILRVSVNFFCG			20	SLAY-screened peptide P4683			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCCCTATGGCCCCGGCTATGTCCTTATCCTGCGTGTGAGCGTGAATTTCTTTTGCGGCTAA	IPYGPGYVLILRVSVNFFCG*	-1.226	0.01391	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26334	SLFDDTPRYTWSSANNYLYC			20	SLAY-screened peptide P4684			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTTGTTTGATGACACGCCGCGCTACACCTGGTCTTCCGCCAACAATTATCTGTATTGCTAA	SLFDDTPRYTWSSANNYLYC*	-1.226	0.000132	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26335	AHSSFINHCHFCIAYPVNTR			20	SLAY-screened peptide P4685			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCACAGCAGCTTCATTAACCACTGTCATTTTTGCATTGCCTATCCCGTTAATACGAGGTAA	AHSSFINHCHFCIAYPVNTR*	-1.226	0.000793	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26336	RVPKCLLPGATVPLYMVVTA			20	SLAY-screened peptide P4686			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGGTTCCGAAGTGTCTCCTGCCGGGCGCTACCGTCCCCCTTTATATGGTTGTCACGGCGTAA	RVPKCLLPGATVPLYMVVTA*	-1.226	0.000682	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26337	PRPMSVNVTSVDV			13	SLAY-screened peptide P4687			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGTCCCATGTCGGTTAATGTTACTTCCGTTGATGTCTAGATCCATCCTGGTAGGCGGTAA	PRPMSVNVTSVDV*IHPGRR*	-1.225	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26338	PRPTNANFGS			10	SLAY-screened peptide P4688			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGCCCCACCAACGCGAACTTTGGCTCCTAGTAGCGTTAGTACAGTGCCGGTATCTGTTAA	PRPTNANFGS**R*YSAGIC*	-1.225	0.00891	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26339	KPCIACTYRSLSPALLYINL			20	SLAY-screened peptide P4689			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCCGTGCATTGCGTGTACTTACCGGTCTCTGTCCCCTGCCTTGCTTTACATTAACCTGTAA	KPCIACTYRSLSPALLYINL*	-1.225	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26340	PMGVKIEALRPAEGNCTAAI			20	SLAY-screened peptide P4690			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATGGGCGTTAAGATTGAGGCCCTTAGGCCCGCTGAGGGCAATTGCACGGCTGCGATTTAA	PMGVKIEALRPAEGNCTAAI*	-1.225	0.003964	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26341	LQHETVH			7	SLAY-screened peptide P4691			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCAGCATGAGACTGTCCATTAGCTCTATCATTTGCCTACTCAGCGTCATCACTCCCACTAA	LQHETVH*LYHLPTQRHHSH*	-1.225	0.001547	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26342	LSGIGPYCPLVAHGSASTGQ			20	SLAY-screened peptide P4692			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTCTGGCATCGGGCCTTACTGTCCCCTTGTCGCTCATGGCTCTGCCAGCACGGGTCAGTAA	LSGIGPYCPLVAHGSASTGQ*	-1.225	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26343	PARPSGTIY			9	SLAY-screened peptide P4693			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCTAGGCCGTCGGGCACGATTTACTAGCCCATCTAGACCCCCTCCTCGTTCACTCGTTAA	PARPSGTIY*PI*TPSSFTR*	-1.224	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26344	PGHKYRALVNIQFNSHWYSR			20	SLAY-screened peptide P4694			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGGCCACAAGTATCGGGCGCTGGTCAACATTCAGTTCAACAGTCACTGGTACAGCCGTTAA	PGHKYRALVNIQFNSHWYSR*	-1.224	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26345	HKLQRVRRDTTDPLSGFRRH			20	SLAY-screened peptide P4695			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAAGCTCCAGAGGGTCCGCCGCGATACTACCGACCCGCTGTCCGGCTTTCGCCGGCACTAA	HKLQRVRRDTTDPLSGFRRH*	-1.224	0.013347	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26346	CLLCHSFHLWRFTVIVLIALN			21	SLAY-screened peptide P4696			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTTGCTGTGCCACAGCTTCCACCTGTGGAGATTCACTGTGATCGTACTTATAGCCCTTAAC	CLLCHSFHLWRFTVIVLIALN	-1.224	0.000104	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26347	EPSVTRTYWPQCITPEYASI			20	SLAY-screened peptide P4697			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCCCTCTGTCACTAGGACCTACTGGCCGCAGTGTATCACGCCGGAGTATGCTAGCATTTAA	EPSVTRTYWPQCITPEYASI*	-1.223	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26348	TSHASHFTYHLSESQISNFY			20	SLAY-screened peptide P4698			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTCTCATGCGTCCCACTTCACTTACCACCTTTCTGAGAGTCAGATTTCTAATTTTTATTAA	TSHASHFTYHLSESQISNFY*	-1.223	0.046408	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26349	TTTAPSACKLNYTFCPTLIA			20	SLAY-screened peptide P4699			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGACTACCGCTCCTTCGGCCTGTAAGCTTAATTATACTTTTTGCCCGACTCTGATCGCGTAA	TTTAPSACKLNYTFCPTLIA*	-1.223	0.015809	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26350	CANRNMLPCLLDLVLVSANV			20	SLAY-screened peptide P4700			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCTAACCGCAACATGCTCCCGTGCCTTCTGGATCTGGTGCTCGTTTCGGCTAATGTCTAA	CANRNMLPCLLDLVLVSANV*	-1.223	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26351	TSYILRLFRFLTVTRRYFSN			20	SLAY-screened peptide P4701			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTCCTATATTCTTCGCCTGTTTCGTTTCCTCACTGTTACTCGCCGCTATTTCTCGAACTAA	TSYILRLFRFLTVTRRYFSN*	-1.223	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26352	LPSRCSRVPFSSPDSQSAVS			20	SLAY-screened peptide P4702			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCGTCGAGGTGCAGTCGGGTCCCTTTTAGTTCCCCCGACAGCCAGTCTGCTGTCAGTTAA	LPSRCSRVPFSSPDSQSAVS*	-1.222	0.019735	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26353	LYIKSTRNSYFLTSRL			16	SLAY-screened peptide P4703			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTACATCAAGTCTACCCGGAATTCGTACTTTCTCACTTCCCGTCTCTAGCTTAGTTATTAA	LYIKSTRNSYFLTSRL*LSY*	-1.222	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26354	LRYNLPAAPLCSDAYSPWTT			20	SLAY-screened peptide P4704			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCGGTATAACCTCCCTGCCGCGCCTTTGTGCAGCGACGCCTACAGCCCGTGGACCACCTAA	LRYNLPAAPLCSDAYSPWTT*	-1.222	0.000357	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26355	CCPTCDHCVPYYSCRLAHYA			20	SLAY-screened peptide P4705			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTGTCCTACCTGTGACCACTGTGTTCCGTATTACTCGTGTCGGCTTGCGCACTACGCCTAA	CCPTCDHCVPYYSCRLAHYA*	-1.222	0.024909	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26356	TSIWRFPILLYLKRHFFRFL			20	SLAY-screened peptide P4706			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAGCATTTGGCGTTTCCCCATTCTGCTCTATTTGAAGCGCCATTTTTTTCGCTTTTTGTAA	TSIWRFPILLYLKRHFFRFL*	-1.222	0.040935	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26357	LPDTSFSTVCHCMD			14	SLAY-screened peptide P4707			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCTGACACGAGTTTTAGTACTGTCTGTCACTGCATGGACTAGGGGGACAAGATGACCTAA	LPDTSFSTVCHCMD*GDKMT*	-1.222	0.023763	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26358	SGAALFYMPCYAPVSTHMA			19	SLAY-screened peptide P4708			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGGCGCCGCCTTGTTTTACATGCCCTGTTACGCGCCCGTGTCCACTCACATGGCGTAGTAA	SGAALFYMPCYAPVSTHMA**	-1.222	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26359	VLPPPHQFRRHRRRGLCPCL			20	SLAY-screened peptide P4709			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCTCCCGCCTCCTCACCAGTTTCGTCGGCATCGCCGTCGTGGCCTGTGCCCGTGCCTTTAA	VLPPPHQFRRHRRRGLCPCL*	-1.222	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26360	HEGISPFQSSHLNDPFTCHT			20	SLAY-screened peptide P4710			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGAGGGGATTTCCCCCTTTCAGAGTAGTCATCTTAATGATCCCTTTACGTGTCATACTTAA	HEGISPFQSSHLNDPFTCHT*	-1.222	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26361	VTLMLRIVATLVGIRLIRRTN			21	SLAY-screened peptide P4711			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCACACTTATGTTGCGAATAGTCGCTACTTTAGTAGGAATACGACTGATCAGACGTACTAAC	VTLMLRIVATLVGIRLIRRTN	-1.222	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26362	GAYVHFLFVVRNRPVHHNVG			20	SLAY-screened peptide P4712			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTGCCTACGTTCATTTCTTGTTCGTCGTCCGCAACAGGCCTGTCCATCATAACGTGGGTTAA	GAYVHFLFVVRNRPVHHNVG*	-1.222	0.000842	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26363	LLSPFVGPTISYILYSLIQK			20	SLAY-screened peptide P4713			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCTGAGTCCGTTCGTGGGCCCCACGATCTCCTACATCCTTTACTCCCTCATTCAGAAGTAA	LLSPFVGPTISYILYSLIQK*	-1.221	0.009695	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26364	PTNASPISGTSLSSTDSSHT			20	SLAY-screened peptide P4714			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACCAACGCGAGCCCTATTTCCGGCACCTCCCTCTCTTCCACTGATAGCTCGCACACCTAA	PTNASPISGTSLSSTDSSHT*	-1.221	0.004022	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26365	AAGTRTNPVMDKTTRDAAHQ			20	SLAY-screened peptide P4715			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGCGGGTACGAGGACTAACCCTGTTATGGACAAGACCACCCGCGATGCCGCTCATCAGTAA	AAGTRTNPVMDKTTRDAAHQ*	-1.22	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26366	RALIVLPTYNRSSHQSFNPA			20	SLAY-screened peptide P4716			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGCGCTGATCGTGCTTCCCACGTACAACCGCTCGAGTCACCAGAGCTTTAACCCCGCGTAA	RALIVLPTYNRSSHQSFNPA*	-1.22	2.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26367	RIPYIWLLHEYSPYNYDNIS			20	SLAY-screened peptide P4717			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATTCCCTATATCTGGCTCCTCCACGAGTATAGCCCGTATAATTACGACAATATCAGCTAA	RIPYIWLLHEYSPYNYDNIS*	-1.22	0.002479	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26368	GPHYDRSHNYPYILTLYDCT			20	SLAY-screened peptide P4718			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCCGCATTATGATAGGAGCCACAATTACCCCTATATCCTTACTCTGTACGACTGTACGTAA	GPHYDRSHNYPYILTLYDCT*	-1.22	0.021208	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26369	APVRANSFYLTWPPSDHPSC			20	SLAY-screened peptide P4719			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCCTGTTCGGGCTAATTCTTTCTATTTGACGTGGCCCCCCTCCGACCACCCCAGTTGCTAA	APVRANSFYLTWPPSDHPSC*	-1.22	2.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26370	TPKRKYCKSSLFKLFNLFFR			20	SLAY-screened peptide P4720			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCTAAGCGTAAGTACTGCAAGTCGTCGCTCTTCAAGTTGTTTAATTTGTTTTTCAGGTAA	TPKRKYCKSSLFKLFNLFFR*	-1.22	0.017482	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26371	PTDFLFDSPRNIYRHYVS			18	SLAY-screened peptide P4721			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACTGACTTTCTGTTTGATTCTCCGCGGAATATCTACCGCCATTACGTTTCCTAGTCCTAA	PTDFLFDSPRNIYRHYVS*S*	-1.219	0.033381	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26372	GLTRFHGCVYILFIISVRIR			20	SLAY-screened peptide P4722			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCTCACCAGGTTCCACGGCTGCGTCTATATTCTCTTTATTATTTCGGTTCGCATCAGGTAA	GLTRFHGCVYILFIISVRIR*	-1.219	0.000221	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26373	YTSVPRSTHTPCAFALFDGL			20	SLAY-screened peptide P4723			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACTTCGGTGCCTCGTTCTACGCACACGCCGTGCGCCTTTGCGCTTTTCGATGGTCTGTAA	YTSVPRSTHTPCAFALFDGL*	-1.219	0.001219	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26374	AGFRYFPSPAPTWIYRKQQS			20	SLAY-screened peptide P4724			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGGCTTCAGGTATTTCCCCAGCCCCGCGCCGACGTGGATCTATCGTAAGCAGCAGTCCTAA	AGFRYFPSPAPTWIYRKQQS*	-1.218	0.018978	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26375	PRLPCGSDQSRVPPITNSRR			20	SLAY-screened peptide P4725			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGGCTCCCTTGTGGTTCCGACCAGAGCCGCGTCCCCCCCATTACGAACTCTCGTCGTTAA	PRLPCGSDQSRVPPITNSRR*	-1.218	0.024526	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26376	VSRCPERLHDQSHYSGNRGT			20	SLAY-screened peptide P4726			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCAGCCGTTGCCCGGAGCGGCTCCATGACCAGAGTCATTACTCTGGGAACCGTGGTACGTAA	VSRCPERLHDQSHYSGNRGT*	-1.218	0.000108	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26377	GHCRAMRLTLAISFGGVPRGN			21	SLAY-screened peptide P4727			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCATTGCCGGGCTATGAGATTGACACTAGCAATTAGCTTCGGGGGAGTGCCAAGGGGTAAC	GHCRAMRLTLAISFGGVPRGN	-1.218	0.000473	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26378	SPCYDWTLSSIIYHTVTNYS			20	SLAY-screened peptide P4728			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCCGTGCTATGACTGGACCCTTAGCTCTATTATTTACCATACGGTTACGAACTATAGTTAA	SPCYDWTLSSIIYHTVTNYS*	-1.218	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26379	LSSHLH			6	SLAY-screened peptide P4729			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCTTCTCACCTGCACTAGAAGTGTATGATCGAGTCTAGTGCCTCGACCACCGCTCCTTAA	LSSHLH*KCMIESSASTTAP*	-1.217	0.010344	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26380	ALQCFKSLVSHIVHSDHQQN			20	SLAY-screened peptide P4730			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCTGCAGTGCTTTAAGAGTTTGGTGTCCCATATTGTTCACAGCGATCACCAGCAGAACTAA	ALQCFKSLVSHIVHSDHQQN*	-1.217	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26381	LYNQISPTVLILPCTFLPDL			20	SLAY-screened peptide P4731			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTATAACCAGATTAGCCCTACTGTTCTTATTCTGCCGTGTACGTTCTTGCCTGATCTTTAA	LYNQISPTVLILPCTFLPDL*	-1.217	0.001025	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26382	CSAPSGARMPAIILASLRFTN			21	SLAY-screened peptide P4732			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTCGGCGCCGAGCGGTGCTCGAATGCCCGCGATTATTCTCGCCTCGCTGCGGTTTACTAAC	CSAPSGARMPAIILASLRFTN	-1.217	0.00137	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26383	NTVCIPYDNFVFSGHLRFAS			20	SLAY-screened peptide P4733			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACACCGTTTGCATTCCCTACGACAACTTTGTTTTCAGTGGGCACTTGAGGTTTGCTTCCTAA	NTVCIPYDNFVFSGHLRFAS*	-1.217	7.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26384	CGKSDFTNESRVHF			14	SLAY-screened peptide P4734			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGGCAAGTCGGATTTCACCAACGAGTCGCGCGTTCATTTCTAGCCCTACTGTTCCCAGTAA	CGKSDFTNESRVHF*PYCSQ*	-1.217	0.025664	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26385	TPVLALCYLCGAHTVDLICV			20	SLAY-screened peptide P4735			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCCGGTTCTTGCCCTCTGTTACTTGTGCGGTGCTCACACGGTCGATCTTATCTGCGTTTAA	TPVLALCYLCGAHTVDLICV*	-1.217	2.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26386	LLNVGRHQGNVVIHLSNFSL			20	SLAY-screened peptide P4736			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCTCAATGTTGGCCGGCACCAGGGCAACGTCGTCATCCACCTTTCTAACTTCAGCCTCTAA	LLNVGRHQGNVVIHLSNFSL*	-1.217	0.020944	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26387	ASVRPFTCYDPAPLPTLLVRN			21	SLAY-screened peptide P4737			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTCCGTTCGTCCTTTTACCTGCTATGATCCGGCGCCACTTCCTACTTTGCTCGTCCGTAAC	ASVRPFTCYDPAPLPTLLVRN	-1.216	0.009957	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26388	LHTFALIRNTTRVYP			15	SLAY-screened peptide P4738			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCATACCTTTGCCTTGATCCGTAATACTACCAGGGTGTACCCGTAGTGTAAGCTCAGCTAA	LHTFALIRNTTRVYP*CKLS*	-1.216	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26389	TLPSNPHCNSLATYLFYLSY			20	SLAY-screened peptide P4739			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTCCCCAGTAATCCTCACTGTAACAGCCTGGCTACTTACCTTTTCTACCTGTCCTATTAA	TLPSNPHCNSLATYLFYLSY*	-1.216	0.034873	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26390	ESLRRISACTAIPHHSLFKS			20	SLAY-screened peptide P4740			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGGGGAGAGAGTCTGCGTCGCATTAGTGCGTGCACCGCCATTCCCCACCATAGCCTCTTTAAGTCTTAA	ESLRRISACTAIPHHSLFKS*	-1.216	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26391	TQYSLSSCKINGYNPWTTAPN			21	SLAY-screened peptide P4741			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCAGTACAGCCTGTCCTCTTGTAAGATTAATGGTTACAACCCGTGGACGACCGCCCCTAAC	TQYSLSSCKINGYNPWTTAPN	-1.216	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26392	DPGVRINPNVRPFIRNTYIW			20	SLAY-screened peptide P4742			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCCCGGTGTTAGGATCAACCCTAATGTGAGGCCCTTCATTCGTAACACGTATATCTGGTAA	DPGVRINPNVRPFIRNTYIW*	-1.216	0.005607	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26393	PNVTFVIVTGYSLHTTWWLP			20	SLAY-screened peptide P4743			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAATGTCACTTTCGTTATTGTGACGGGCTACAGCCTTCATACGACGTGGTGGCTGCCTTAA	PNVTFVIVTGYSLHTTWWLP*	-1.216	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26394	SCSPHCLVYPVFPDTFHRCR			20	SLAY-screened peptide P4744			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTGCTCGCCGCACTGTCTCGTCTACCCTGTCTTCCCTGATACGTTTCACCGTTGTCGTTAA	SCSPHCLVYPVFPDTFHRCR*	-1.216	0.018472	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26395	RIMVPYFHFI			10	SLAY-screened peptide P4745			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTATTATGGTTCCTTATTTTCACTTCATTTAGCATTGCCCTATTACGATTCATCGTAACTAA	RIMVPYFHFI*HCPITIHRN*	-1.215	0.005437	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26396	VLILLDHQNWSSCPSLSFIR			20	SLAY-screened peptide P4746			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCTTATTCTTCTGGACCACCAGAACTGGTCGAGCTGCCCTAGCCTCTCCTTCATCCGTTAA	VLILLDHQNWSSCPSLSFIR*	-1.215	0.035413	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26397	TRTYKRLWSLIMTMVTRHCT			20	SLAY-screened peptide P4747			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCGCACGTATAAGCGGCTTTGGTCTCTCATCATGACCATGGTCACGCGTCATTGCACGTAA	TRTYKRLWSLIMTMVTRHCT*	-1.215	0.033917	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26398	LQVRCRIVVVNLTFSWYLWV			20	SLAY-screened peptide P4748			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCAGGTGAGGTGCCGGATTGTTGTCGTTAATTTGACTTTTTCCTGGTACTTGTGGGTTTAA	LQVRCRIVVVNLTFSWYLWV*	-1.214	0.000361	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26399	LHCSLAIPSLRPASHETLDS			20	SLAY-screened peptide P4749			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCACTGTAGCCTGGCGATCCCGTCCCTTCGCCCCGCTTCGCACGAGACTCTTGACAGCTAA	LHCSLAIPSLRPASHETLDS*	-1.214	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26400	RHQPYCATPSFLLLACYKDT			20	SLAY-screened peptide P4750			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCACCAGCCCTACTGTGCCACGCCCTCTTTCCTTCTTCTCGCTTGTTATAAGGACACTTAA	RHQPYCATPSFLLLACYKDT*	-1.213	0.03173	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26401	PPCVWRPTWAMRPSYISSYP			20	SLAY-screened peptide P4751			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCTGTGTTTGGCGCCCTACGTGGGCCATGCGTCCTTCGTACATCAGCTCTTATCCTTAA	PPCVWRPTWAMRPSYISSYP*	-1.213	0.026568	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26402	ARDPYLNRCHCYSLNSYFLL			20	SLAY-screened peptide P4752			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAGGGATCCCTATCTTAACCGCTGTCACTGTTACTCTCTGAACTCCTACTTTTTGCTTTAA	ARDPYLNRCHCYSLNSYFLL*	-1.213	0.048564	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26403	QHPECIDLNFGGLDR			15	SLAY-screened peptide P4753			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCACCCTGAGTGCATCGATCTTAATTTTGGTGGCCTGGACAGGTAGATGAGCTTCCAGTAA	QHPECIDLNFGGLDR*MSFQ*	-1.213	0.021488	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26404	SYSGSWNLAPCIVRRPLWIY			20	SLAY-screened peptide P4754			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTATTCTGGCTCCTGGAATCTTGCCCCTTGCATTGTTCGCCGCCCGCTCTGGATTTACTAA	SYSGSWNLAPCIVRRPLWIY*	-1.213	0.03036	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26405	PCHGSELN			8	SLAY-screened peptide P4755			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGTCACGGTTCCGAGCTTAATTAGCATATCTATTATGCCTATTATCTGCCTTTCTCCTAA	PCHGSELN*HIYYAYYLPFS*	-1.213	0.002872	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26406	LWHILALLMSRISFPPIPTR			20	SLAY-screened peptide P4756			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTGGCACATTCTTGCCCTTCTGATGTCGCGCATCTCGTTCCCCCCGATCCCTACTCGGTAA	LWHILALLMSRISFPPIPTR*	-1.212	0.000251	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26407	LPHWNHTR			8	SLAY-screened peptide P4757			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCCTCACTGGAACCACACCCGGTAGGCCAGCCTCAAGTATCCCACCTGTGCGGATTTGTAA	LPHWNHTR*ASLKYPTCADL*	-1.212	0.017062	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26408	YCNPFWIYPSYSRNIIV			17	SLAY-screened peptide P4758			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTGTAATCCGTTCTGGATTTACCCCTCGTACAGCAGGAATATCATTGTCTAGCTTGCCTAA	YCNPFWIYPSYSRNIIV*LA*	-1.212	9.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26409	ITRNGRILSLSHPSRRYTIS			20	SLAY-screened peptide P4759			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCACTCGGAACGGTCGTATTCTGTCTCTTTCCCACCCTTCTCGCCGTTATACCATTAGTTAA	ITRNGRILSLSHPSRRYTIS*	-1.212	0.001218	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26410	RNIPYNAECSSLPSRRTNSD			20	SLAY-screened peptide P4760			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAACATCCCGTACAATGCGGAGTGTAGCAGTTTGCCTAGCCGCCGGACGAATTCTGACTAA	RNIPYNAECSSLPSRRTNSD*	-1.212	0.019009	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26411	LRSRPPRKIPRTFATTQRLF			20	SLAY-screened peptide P4761			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCGGTCTCGCCCGCCTCGGAAGATCCCCAGGACTTTCGCCACTACCCAGCGCCTCTTTTAA	LRSRPPRKIPRTFATTQRLF*	-1.212	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26412	RVFCD			5	SLAY-screened peptide P4762			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGTTTTTTGTGATTAGAATTCCACTTATGTCCTTACCCACTTTATTAACCCCTCGGTTTAA	RVFCD*NSTYVLTHFINPSV*	-1.211	0.00028	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26413	ASHYSFRTAPISIFAYGFYS			20	SLAY-screened peptide P4763			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTCGCATTACAGTTTCCGCACCGCTCCGATTTCTATCTTCGCTTACGGCTTCTATTCTTAA	ASHYSFRTAPISIFAYGFYS*	-1.211	0.000705	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26414	HLPGLNFDWHNLFLPYNTPC			20	SLAY-screened peptide P4764			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTTGCCGGGGCTTAACTTCGACTGGCATAATCTTTTCCTTCCTTATAACACTCCTTGCTAA	HLPGLNFDWHNLFLPYNTPC*	-1.211	0.008433	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26415	SERISPINNYHAGSSNRQLG			20	SLAY-screened peptide P4765			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGAGCGCATTAGTCCGATCAATAATTACCATGCTGGTTCCAGTAATAGGCAGCTTGGCTAA	SERISPINNYHAGSSNRQLG*	-1.211	0.009057	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26416	NHHHGCTLTTYI			12	SLAY-screened peptide P4766			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCATCATCACGGTTGTACCCTTACCACGTATATTTAGCCCCACATTAGGACTTCGCAGTAA	NHHHGCTLTTYI*PHIRTSQ*	-1.21	8.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26417	PGPLRIVNPCHITCCLNKNH			20	SLAY-screened peptide P4767			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGGGCCCCTCAGGATCGTCAACCCTTGTCATATCACTTGCTGTCTGAACAAGAATCATTAA	PGPLRIVNPCHITCCLNKNH*	-1.21	0.000106	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26418	NGTARNLLIRIHSTNFLGF			19	SLAY-screened peptide P4768			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGGCACCGCCCGCAATCTTCTTATTAGGATCCATTCCACGAACTTTCTTGGTTTTTAGTAA	NGTARNLLIRIHSTNFLGF**	-1.21	0.000501	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26419	TPSD			4	SLAY-screened peptide P4769			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCCCAGTGACTAGTATGACAGGGACCCTTGCTTCCTGTAGCCCAACTAGTGTGGCATTTAC	TPSD*YDRDPCFL*PN*CGIY	-1.21	0.043255	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26420	TLCPFYQFYSLYGKGSSGSL			20	SLAY-screened peptide P4770			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTGTGTCCGTTTTATCAGTTTTACTCGCTTTACGGCAAGGGCTCTTCTGGCTCCTTGTAA	TLCPFYQFYSLYGKGSSGSL*	-1.209	0.004689	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26421	TPVYGDDAYSNIPGHPAPNT			20	SLAY-screened peptide P4771			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCCCGTGTACGGTGACGACGCCTACTCTAATATTCCCGGGCACCCCGCGCCTAACACTTAA	TPVYGDDAYSNIPGHPAPNT*	-1.209	0.000442	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26422	FSP			3	SLAY-screened peptide P4772			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTCCCCTTAGACTACCCATCCTTTTAAGGCTTTGCCTAACCATTGGAATGTTCTCAACTAA	FSP*TTHPFKALPNHWNVLN*	-1.209	0.031545	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26423	GYSNTVRFLIPDWTRPNWGN			20	SLAY-screened peptide P4773			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTACTCTAATACGGTCCGTTTCCTTATTCCCGATTGGACTCGCCCGAATTGGGGGAATTAA	GYSNTVRFLIPDWTRPNWGN*	-1.209	0.000831	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26424	HPQTCFRTPSAKPIRG			16	SLAY-screened peptide P4774			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCTCAGACTTGCTTCCGGACGCCCAGTGCTAAGCCCATCCGCGGTTAGACCCGCGAGTAA	HPQTCFRTPSAKPIRG*TRE*	-1.209	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26425	RSLLRFTPGCEAFYLIPFRP			20	SLAY-screened peptide P4775			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAGCCTCCTTCGGTTCACGCCCGGGTGTGAGGCGTTTTATCTGATTCCTTTCCGTCCGTAA	RSLLRFTPGCEAFYLIPFRP*	-1.209	0.035389	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26426	CYPWVPTVMLVASTPDNHIN			20	SLAY-screened peptide P4776			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTACCCCTGGGTCCCCACGGTGATGCTGGTTGCTTCCACCCCCGACAATCATATCAACTAA	CYPWVPTVMLVASTPDNHIN*	-1.209	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26427	LLNQLSMYRHYLARKLGASL			20	SLAY-screened peptide P4777			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCTCAATCAGCTGAGCATGTATCGCCACTACCTCGCTCGGAAGCTTGGTGCGTCTCTTTAA	LLNQLSMYRHYLARKLGASL*	-1.209	0.029887	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26428	LHFSQLPLHVI			11	SLAY-screened peptide P4778			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCATTTCTCGCAGCTGCCTCTGCACGTCATATGATTGCCATGTATGATCACAATATGTAAC	LHFSQLPLHVI*LPCMITICN	-1.209	0.001233	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26429	HIGLCDTHSLPYETSRHLSS			20	SLAY-screened peptide P4779			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATATCGGCCTGTGTGACACCCATAGTCTCCCTTATGAGACTTCCCGTCACCTGTCGAGCTAA	HIGLCDTHSLPYETSRHLSS*	-1.209	0.031503	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26430	CHCPLQTNNAHLTFTTRCSSN			21	SLAY-screened peptide P4780			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCATTGCCCTCTGCAGACCAATAACGCTCATTTGACGTTTACTACCAGGTGTAGTAGTAAC	CHCPLQTNNAHLTFTTRCSSN	-1.208	4.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26431	TNRCLRSFFLCLN			13	SLAY-screened peptide P4781			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAACCGTTGCCTTAGGTCCTTTTTTTTGTGCCTGAATTAGCCTTTCGTTAGGTTCCACTAA	TNRCLRSFFLCLN*PFVRFH*	-1.208	0.000545	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26432	TPFPYYILPYCTGWTPLVTE			20	SLAY-screened peptide P4782			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCGTTCCCCTATTATATCCTCCCCTATTGTACGGGGTGGACTCCCCTGGTAACTGAGTAA	TPFPYYILPYCTGWTPLVTE*	-1.208	0.029131	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26433	LIPWIDYHPCHYQCSTLHWR			20	SLAY-screened peptide P4783			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCATCCCTTGGATCGATTATCATCCTTGTCACTACCAGTGTTCCACTCTTCATTGGCGCTAA	LIPWIDYHPCHYQCSTLHWR*	-1.208	0.004962	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26434	RFVCQANVREIITFILNSCY			20	SLAY-screened peptide P4784			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTTCGTGTGCCAGGCCAATGTTCGCGAGATTATTACTTTTATTCTTAATAGCTGCTATTAA	RFVCQANVREIITFILNSCY*	-1.208	0.045201	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26435	IPHIFKWTRTLHSAGSRIMLY			21	SLAY-screened peptide P4785			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCCCCATATCTTTAAGTGGACCCGTACCCTTCACTCTGCCGGCTCTCGCATCATGCTTTAC	IPHIFKWTRTLHSAGSRIMLY	-1.207	0.040611	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26436	PAPPPFSRSSISPQHSLVSG			20	SLAY-screened peptide P4786			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCTCCGCCCCCCTTTAGCCGTAGCTCTATTAGCCCCCAGCACTCCTTGGTGTCGGGCTAA	PAPPPFSRSSISPQHSLVSG*	-1.207	0.002253	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26437	FRGS			4	SLAY-screened peptide P4787			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCGCGGGTCGTAGTGCGATAATCCTCGTGGCAGGTGGAACCCGAATTAGCCTTACTCTTAC	FRGS*CDNPRGRWNPN*PYSY	-1.207	0.021189	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26438	SPSSLAPWSCPDHFYLALMS			20	SLAY-screened peptide P4788			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCCGTCGTCTCTCGCGCCCTGGTCTTGCCCCGACCATTTTTACCTCGCTCTTATGAGTTAA	SPSSLAPWSCPDHFYLALMS*	-1.207	0.007592	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26439	QADDPAPGAYTSCVSYDAPS			20	SLAY-screened peptide P4789			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGCTGATGATCCCGCTCCCGGGGCTTACACCTCTTGCGTTTCTTATGACGCTCCTAGCTAA	QADDPAPGAYTSCVSYDAPS*	-1.207	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26440	HNGQRKSCSYLCDRLVPKIH			20	SLAY-screened peptide P4790			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAACGGCCAGCGTAAGTCGTGTAGTTATTTGTGTGACCGGCTCGTTCCGAAGATTCATTAA	HNGQRKSCSYLCDRLVPKIH*	-1.206	0.026248	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26441	PTPPTISGETASVVPSSSTP			20	SLAY-screened peptide P4791			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACTCCTCCTACCATTTCTGGCGAGACTGCCTCTGTTGTCCCTAGTAGTAGTACGCCGTAA	PTPPTISGETASVVPSSSTP*	-1.206	8.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26442	RNSETAFGD			9	SLAY-screened peptide P4792			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAATTCCGAGACGGCGTTTGGCGACTAGTAGAGTCTGCTCAGCTCCGCCCGTACCTTCTAA	RNSETAFGD**SLLSSARTF*	-1.206	0.016225	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26443	APSPAYCHNIRTEFSRHWTL			20	SLAY-screened peptide P4793			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCGAGCCCGGCCTACTGTCATAACATCCGTACTGAGTTTAGCAGGCACTGGACTTTGTAA	APSPAYCHNIRTEFSRHWTL*	-1.205	0.000153	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26444	SGAAHSNRLYTPTHLRMTST			20	SLAY-screened peptide P4794			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGGTGCCGCCCACAGCAATCGTCTTTATACCCCTACCCATCTGCGGATGACGTCGACTTAA	SGAAHSNRLYTPTHLRMTST*	-1.205	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26445	TMAPHMFHR			9	SLAY-screened peptide P4795			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTATGGCGCCGCATATGTTTCATAGGTAGAACAGTGCTTGTGTTTTTTTTTTGAATCATAAC	TMAPHMFHR*NSACVFFLNHN	-1.205	0.000139	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26446	NAART			5	SLAY-screened peptide P4796			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGCGGCGAGGACGTAGAGCCTGAGTTCTCTTGTTTGTAATAACCTTTCTTTCGTCTTGTAA	NAART*SLSSLVCNNLSFVL*	-1.205	0.014001	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26447	FYYRLSAHIHPTSILK			16	SLAY-screened peptide P4797			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTATTACCGGTTGTCTGCGCACATTCACCCTACCTCCATTCTTAAGTAGACGGATTACTAA	FYYRLSAHIHPTSILK*TDY*	-1.205	0.000112	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26448	PSNPPTRCLSAVNCTPHCGY			20	SLAY-screened peptide P4798			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGTAACCCCCCTACGCGTTGCCTGTCGGCCGTTAACTGCACTCCTCACTGCGGCTACTAA	PSNPPTRCLSAVNCTPHCGY*	-1.204	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26449	NPVYFPLCGVWNCCPYAPPR			20	SLAY-screened peptide P4799			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCCCGTCTACTTCCCCCTTTGTGGTGTTTGGAATTGCTGTCCGTATGCCCCCCCTCGCTAA	NPVYFPLCGVWNCCPYAPPR*	-1.204	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26450	TVWPPWPRIYCLHIWLSSTN			20	SLAY-screened peptide P4800			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGTCTGGCCTCCCTGGCCTAGGATTTATTGTCTTCACATTTGGCTCAGCAGTACTAACTAA	TVWPPWPRIYCLHIWLSSTN*	-1.204	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26451	KPALPSSLYPFHVCPGF			17	SLAY-screened peptide P4801			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCCTGCGCTGCCCTCTTCTCTTTATCCCTTCCACGTCTGCCCTGGGTTTTAGTAGAGTTAA	KPALPSSLYPFHVCPGF**S*	-1.204	0.000321	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26452	RNAPIDRMFLKLTPNRDTMS			20	SLAY-screened peptide P4802			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGAACGCTCCTATCGACCGTATGTTTCTTAAGCTGACTCCCAACAGGGACACTATGTCGTAA	RNAPIDRMFLKLTPNRDTMS*	-1.204	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26453	ISLNL			5	SLAY-screened peptide P4803			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAGCCTTAACCTCTAGTTCTTGGACGCCGGCTTTTCTAGCAAGCTTCCTTTTGGTGAGTAA	ISLNL*FLDAGFSSKLPFGE*	-1.204	0.001245	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26454	SSNNLNNCYCFI			12	SLAY-screened peptide P4804			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTCCAACAATCTCAATAATTGCTACTGTTTCATTTAGTACCGTGTTCACACCAGGTTTTAA	SSNNLNNCYCFI*YRVHTRF*	-1.204	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26455	CLYDWRYYYTVRNL			14	SLAY-screened peptide P4805			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTCTATGACTGGAGGTATTACTACACCGTCCGTAATCTCTAGTTTTTGTTCTGTACCTAA	CLYDWRYYYTVRNL*FLFCT*	-1.203	0.027292	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26456	HTSEFMNYLRPSRNNPFICR			20	SLAY-screened peptide P4806			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACGTCTGAGTTCATGAATTATCTTCGCCCTAGTAGGAACAATCCCTTCATTTGCCGGTAA	HTSEFMNYLRPSRNNPFICR*	-1.203	0.00093	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26457	LSTCSYAETTPLHREVHMHV			20	SLAY-screened peptide P4807			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCCACCTGTTCCTACGCTGAGACTACTCCCCTCCATCGTGAGGTGCACATGCACGTCTAA	LSTCSYAETTPLHREVHMHV*	-1.203	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26458	ALARVNNLNLPLLCSDLHNY			20	SLAY-screened peptide P4808			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCTTGCGCGCGTCAATAACCTGAATTTGCCGCTCTTGTGTTCTGATTTGCATAACTACTAA	ALARVNNLNLPLLCSDLHNY*	-1.203	0.015317	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26459	IWTRARSLLIRKRPWPLRGT			20	SLAY-screened peptide P4809			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTGGACGCGGGCCCGCTCCCTCCTTATCCGTAAGAGGCCGTGGCCTCTCCGTGGCACCTAA	IWTRARSLLIRKRPWPLRGT*	-1.203	0.041331	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26460	DHHLTFGHVHDLWNYTLNNA			20	SLAY-screened peptide P4810			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCATCACCTCACGTTTGGCCACGTTCACGACCTCTGGAACTATACCCTGAATAACGCCTAA	DHHLTFGHVHDLWNYTLNNA*	-1.203	0.035248	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26461	CASQIPNATHLIRPIPYGSI			20	SLAY-screened peptide P4811			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGCGTCCCAGATCCCGAACGCTACGCACTTGATTCGCCCCATCCCTTATGGCTCGATCTAA	CASQIPNATHLIRPIPYGSI*	-1.202	0.013939	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26462	PRRLDYPVHY			10	SLAY-screened peptide P4812			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGCAGGCTTGACTACCCCGTCCACTACTAGCCTAAGACGAATTTTTCGGCTAGCTTCTAA	PRRLDYPVHY*PKTNFSASF*	-1.202	0.034808	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26463	SGVPVFSPFGVYHLIVQYAC			20	SLAY-screened peptide P4813			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGGTGTCCCGGTCTTTTCTCCGTTTGGCGTCTACCACCTCATTGTTCAGTATGCCTGTTAA	SGVPVFSPFGVYHLIVQYAC*	-1.201	0.001797	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26464	PNCPALWPTCYISSS			15	SLAY-screened peptide P4814			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAATTGCCCCGCCTTGTGGCCCACTTGTTACATTTCGAGCTCTTAGAATTCCTCTAGGTAA	PNCPALWPTCYISSS*NSSR*	-1.201	0.035525	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26465	VLMSTLSSYLHHLKANRNRT			20	SLAY-screened peptide P4815			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCTTATGTCCACGTTGAGCTCCTATCTTCACCATTTGAAGGCGAATAGGAACCGCACCTAA	VLMSTLSSYLHHLKANRNRT*	-1.201	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26466	WYGYLPTWPYGYPQVAIYSH			20	SLAY-screened peptide P4816			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTATGGCTATCTCCCTACCTGGCCGTATGGGTATCCGCAGGTCGCTATTTATTCCCATTAA	WYGYLPTWPYGYPQVAIYSH*	-1.201	0.001797	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26467	WRCASNVYLHQNTNSNNYPI			20	SLAY-screened peptide P4817			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCGCTGCGCGAGCAACGTCTACCTTCACCAGAACACTAATTCCAATAATTACCCCATTTAA	WRCASNVYLHQNTNSNNYPI*	-1.201	1.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26468	DGPSSCTYFL			10	SLAY-screened peptide P4818			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGGTCCTTCTAGCTGCACTTATTTCTTGTAGTCTGTGAACGCGGGTGTGTGGTCTAGGTAA	DGPSSCTYFL*SVNAGVWSR*	-1.201	0.008829	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26469	CVCPPCYSFINLTPVKDPGL			20	SLAY-screened peptide P4819			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGTCTGCCCGCCTTGCTATTCTTTCATTAACCTTACGCCGGTCAAGGACCCGGGTCTCTAA	CVCPPCYSFINLTPVKDPGL*	-1.201	0.000808	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26470	EDTCASLSGTLITHMCGCLI			20	SLAY-screened peptide P4820			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGATACTTGCGCCAGCCTCTCCGGCACGCTCATCACGCACATGTGCGGTTGCCTGATCTAA	EDTCASLSGTLITHMCGCLI*	-1.2	0.000179	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26471	YQMSTSSPALSA			12	SLAY-screened peptide P4821			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCAGATGTCTACAAGCTCTCCAGCGCTAAGCGCCTGACTCATGTGATTTCGTCGCATTAAC	YQMSTSSPALSA*LM*FRRIN	-1.2	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26472	IYRGGCDIHSIHCSPQMRVQ			20	SLAY-screened peptide P4822			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTATAGGGGCGGTTGTGATATCCACAGTATCCACTGTTCGCCCCAGATGCGTGTTCAGTAA	IYRGGCDIHSIHCSPQMRVQ*	-1.2	0.00083	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26473	AFHSSCPPRYELALNGASGH			20	SLAY-screened peptide P4823			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTTTCACTCCAGCTGCCCCCCTCGTTATGAGCTTGCGTTGAATGGCGCGTCCGGCCATTAA	AFHSSCPPRYELALNGASGH*	-1.199	0.029845	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26474	FPDLCGHNKLYYET			14	SLAY-screened peptide P4824			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCCTGACCTGTGCGGCCATAATAAGCTGTACTATGAGACCTAGATTATTGCTACTCACTAA	FPDLCGHNKLYYET*IIATH*	-1.199	0.02146	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26475	PTTCFYTRFGRLDRGIKYTP			20	SLAY-screened peptide P4825			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACCACTTGTTTTTACACTCGCTTTGGTCGCTTGGATCGGGGCATCAAGTACACTCCCTAA	PTTCFYTRFGRLDRGIKYTP*	-1.199	0.00433	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26476	HTSKNQSWRAYLFWDPQIDF			20	SLAY-screened peptide P4826			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACTAGCAAGAATCAGTCTTGGCGTGCGTACCTTTTTTGGGACCCTCAGATCGACTTTTAA	HTSKNQSWRAYLFWDPQIDF*	-1.198	0.000285	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26477	PIIFMRVSSM			10	SLAY-screened peptide P4827			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATTATCTTCATGAGGGTCAGTTCCATGTAGCCGCTTGCTCTCCCCCACTGTGCTGCGTAA	PIIFMRVSSM*PLALPHCAA*	-1.198	2.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26478	FPYSAWPDCYVLNY			14	SLAY-screened peptide P4828			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCCCTATAGCGCTTGGCCCGACTGTTATGTGCTCAATTACTAGATTATGACCTCCCGCTAA	FPYSAWPDCYVLNY*IMTSR*	-1.197	0.02154	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26479	QRCTCDLPSTCRSLFLYTAT			20	SLAY-screened peptide P4829			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCGCTGCACGTGTGACCTGCCCTCCACCTGTAGGTCTCTCTTTCTGTATACGGCCACTTAA	QRCTCDLPSTCRSLFLYTAT*	-1.197	0.01791	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26480	IVDTDPDSGVDYNCVPTMIA			20	SLAY-screened peptide P4830			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGTGGACACTGACCCCGACAGCGGCGTTGACTACAACTGTGTCCCTACGATGATTGCTTAA	IVDTDPDSGVDYNCVPTMIA*	-1.197	0.006305	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26481	RDIFLVVLDHDDNHSTYDIL			20	SLAY-screened peptide P4831			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGACATTTTCCTTGTCGTGCTTGACCACGATGACAATCATAGTACTTACGACATCTTGTAA	RDIFLVVLDHDDNHSTYDIL*	-1.197	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26482	VAPGPLLILSLILRPLRIRPP			21	SLAY-screened peptide P4832			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGGCCCCGGGACCGCTACTTATACTCTCTTTAATACTCCGCCCATTAAGGATCAGGCCACCT	VAPGPLLILSLILRPLRIRPP	-1.196	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26483	CGFGQSINNLFRIFKSSSGV			20	SLAY-screened peptide P4833			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGGCTTCGGGCAGAGCATCAATAACCTTTTTCGCATTTTCAAGAGTTCTTCTGGCGTGTAA	CGFGQSINNLFRIFKSSSGV*	-1.196	0.000833	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26484	HSTISNPRRCSHATNKSILQ			20	SLAY-screened peptide P4834			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAGCACGATCTCCAATCCGCGGCGCTGCTCTCATGCGACTAACAAGTCTATCCTCCAGTAA	HSTISNPRRCSHATNKSILQ*	-1.196	0.000192	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26485	TPVRTDLIK			9	SLAY-screened peptide P4835			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCGGTGCGCACCGACCTGATCAAATGATCAGTATACGGCGACCTTTACTACTTCCTAACT	TPVRTDLIK*SVYGDLYYFLT	-1.196	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26486	PVCIRTINLGASILNYANYV			20	SLAY-screened peptide P4836			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGTGTGTATTCGTACTATCAACCTCGGGGCCTCTATCCTTAACTACGCGAATTACGTTTAA	PVCIRTINLGASILNYANYV*	-1.196	0.003701	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26487	CAVWCIGTAHYAALMSPGIL			20	SLAY-screened peptide P4837			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCTGTCTGGTGTATCGGCACGGCCCACTACGCCGCCCTGATGTCGCCTGGTATCCTTTAA	CAVWCIGTAHYAALMSPGIL*	-1.195	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26488	FHSLLSWPLMHNRGPKSAVTN			21	SLAY-screened peptide P4838			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCACTCCCTTTTGTCTTGGCCCTTGATGCACAACCGGGGCCCCAAATCAGCAGTAACTAAC	FHSLLSWPLMHNRGPKSAVTN	-1.195	3.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26489	TSSYHINIPYVNSTSVTATR			20	SLAY-screened peptide P4839			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTCTAGTTACCATATTAACATTCCCTATGTTAACTCCACCAGTGTTACTGCGACGCGCTAA	TSSYHINIPYVNSTSVTATR*	-1.195	0.011351	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26490	RGLLRQRDRN			10	SLAY-screened peptide P4840			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGGCCTGCTTCGTCAGCGGGACCGGAATTAGATCTACGTTAATTAGAAGCAGCATCCTTAA	RGLLRQRDRN*IYVN*KQHP*	-1.195	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26491	YICLPSTPGRWGRINYKDSYY			21	SLAY-screened peptide P4841			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACATTTGTTTGCCTTCCACTCCCGGTAGGTGGGGTCGCATTAATTACAAGGATAGCTACTAC	YICLPSTPGRWGRINYKDSYY	-1.195	0.000452	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26492	TFRNNLVVCTYARGPPTVDP			20	SLAY-screened peptide P4842			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTTCCGTAACAACCTTGTCGTCTGCACTTATGCCCGTGGCCCGCCGACTGTCGATCCCTAA	TFRNNLVVCTYARGPPTVDP*	-1.194	0.000143	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26493	RRSPHIERNTLYPLNCLNVV			20	SLAY-screened peptide P4843			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCGCTCGCCGCACATTGAGCGTAACACCCTGTACCCTCTTAATTGCCTCAATGTTGTTTAA	RRSPHIERNTLYPLNCLNVV*	-1.194	0.003589	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26494	LICTSYACLFPHPCFTTFQG			20	SLAY-screened peptide P4844			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCATCTGCACCAGCTACGCGTGCCTTTTCCCTCACCCTTGTTTCACGACTTTCCAGGGGTAA	LICTSYACLFPHPCFTTFQG*	-1.194	0.013804	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26495	NHPVLRATAPSLTLMDSYLI			20	SLAY-screened peptide P4845			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCATCCTGTTCTGCGGGCCACTGCCCCCAGTCTCACTCTTATGGATTCCTATCTCATTTAA	NHPVLRATAPSLTLMDSYLI*	-1.194	0.003587	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26496	LPSSFVGAMKV			11	SLAY-screened peptide P4846			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCTTCTAGCTTTGTGGGGGCTATGAAGGTCTAGGCCAATGCCCTCAGCTGTGATTTGTAA	LPSSFVGAMKV*ANALSCDL*	-1.194	0.00028	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26497	ALCAPSHLGQVVMEDFV			17	SLAY-screened peptide P4847			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTTGTGCGCGCCTTCCCACCTTGGCCAGGTCGTTATGGAGGATTTCGTGTAGTAGATGTAA	ALCAPSHLGQVVMEDFV**M*	-1.194	0.02836	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26498	PTTPPACSKLRPDHNPLYAV			20	SLAY-screened peptide P4848			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACGACTCCCCCGGCTTGCTCTAAGCTTCGTCCTGACCATAATCCTCTGTATGCTGTGTAA	PTTPPACSKLRPDHNPLYAV*	-1.194	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26499	RGHCHTPIYPYHCTPCVIYL			20	SLAY-screened peptide P4849			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGGGTCATTGTCACACTCCCATTTATCCTTATCATTGTACGCCGTGCGTGATTTACCTCTAA	RGHCHTPIYPYHCTPCVIYL*	-1.193	0.031396	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26500	RCNLVADLV			9	SLAY-screened peptide P4850			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGCAACCTCGTGGCCGACCTCGTTTAGTATATGGATAAGCTGCCCCTTAGTGAGTTTTAA	RCNLVADLV*YMDKLPLSEF*	-1.193	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26501	DIRAHTSTLVGRLT			14	SLAY-screened peptide P4851			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACATTAGGGCCCACACCTCCACTTTGGTCGGCCGCCTTACTTAGTTCTACTGGTGGTTCTAA	DIRAHTSTLVGRLT*FYWWF*	-1.193	0.000798	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26502	VGIT			4	SLAY-screened peptide P4852			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGGTATTACCTAGTTTCCGCATTGTTACGATCTTACTACCATTTATATGAACGACCGTTAA	VGIT*FPHCYDLTTIYMNDR*	-1.193	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26503	LRRSFAVPLVGITLTWRDIN			20	SLAY-screened peptide P4853			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCGCCGTTCTTTTGCGGTCCCCCTTGTCGGGATCACGCTGACCTGGCGCGATATTAACTAA	LRRSFAVPLVGITLTWRDIN*	-1.193	7.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26504	PSGLRNDAWHCTPAYLYLFG			20	SLAY-screened peptide P4854			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGCGGCTTGCGCAATGATGCCTGGCACTGTACTCCTGCCTACCTCTACCTGTTTGGCTAA	PSGLRNDAWHCTPAYLYLFG*	-1.193	0.023385	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26505	PSCPSIPRLFLLSNSWNGLK			20	SLAY-screened peptide P4855			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCGTGCCCCAGCATCCCTCGTTTGTTTCTCTTGTCCAACTCCTGGAATGGGCTCAAGTAA	PSCPSIPRLFLLSNSWNGLK*	-1.193	0.018608	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26506	GQTLCIESLVPLVTRRHCVA			20	SLAY-screened peptide P4856			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCAGACCCTCTGTATCGAGAGTCTGGTCCCGCTCGTCACCCGTAGGCATTGCGTTGCCTAA	GQTLCIESLVPLVTRRHCVA*	-1.192	0.013038	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26507	RAQPS			5	SLAY-screened peptide P4857			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGGCCCAGCCCAGTTAGCATATTGCCATCTCTCTTAGTCACCATGCCACGGGCTAACTGAGT	RAQPS*HIAISLSHHATG*LS	-1.192	1.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26508	TQNEITTPSVCTPFVEIHID			20	SLAY-screened peptide P4858			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCAGAACGAGATTACTACGCCCAGTGTTTGCACCCCTTTCGTGGAGATTCATATTGATTAA	TQNEITTPSVCTPFVEIHID*	-1.192	0.021869	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26509	FTHKYSSYGSTGLTQSIGRA			20	SLAY-screened peptide P4859			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCACTCACAAGTATTCTAGCTATGGGTCCACCGGGCTTACCCAGAGTATCGGGCGTGCGTAA	FTHKYSSYGSTGLTQSIGRA*	-1.191	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26510	EDYYNTITTPAGHAAFTSHI			20	SLAY-screened peptide P4860			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGACTACTACAACACCATTACTACCCCGGCTGGGCACGCTGCCTTTACCTCCCATATCTAA	EDYYNTITTPAGHAAFTSHI*	-1.191	0.00445	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26511	GTRHLAPCFPRSPSIVVPSP			20	SLAY-screened peptide P4861			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCACTCGTCACCTCGCCCCGTGCTTCCCTCGTAGTCCGTCTATCGTCGTCCCTAGTCCCTAA	GTRHLAPCFPRSPSIVVPSP*	-1.191	0.001022	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26512	LYLLDFTSGVTYLCIDPSLT			20	SLAY-screened peptide P4862			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTACCTCCTGGACTTCACGTCGGGCGTCACTTACCTTTGTATTGACCCTTCCTTGACTTAA	LYLLDFTSGVTYLCIDPSLT*	-1.191	0.000211	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26513	TINARASGFTNNTRYWIRWV			20	SLAY-screened peptide P4863			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCATCAATGCCAGGGCCTCTGGCTTTACGAACAACACCAGGTATTGGATTCGTTGGGTCTAA	TINARASGFTNNTRYWIRWV*	-1.19	0.044173	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26514	PVSRSRRRRGCYRLRCWNTY			20	SLAY-screened peptide P4864			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGTTTCTAGGAGCCGGCGCAGGCGCGGGTGTTATCGTCTCAGGTGTTGGAATACTTACTAA	PVSRSRRRRGCYRLRCWNTY*	-1.19	0.019833	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26515	VHQTDWANEPYPMHYRATLT			20	SLAY-screened peptide P4865			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCATCAGACCGACTGGGCTAATGAGCCGTACCCTATGCACTACCGTGCTACCCTCACTTAA	VHQTDWANEPYPMHYRATLT*	-1.189	0.000496	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26516	TSYSNGQSTPLV			12	SLAY-screened peptide P4866			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCCTACAGCAACGGTCAGTCGACTCCTTTGGTATAATGATAATGAGACTCTGACCTAACT	TSYSNGQSTPLV****DSDLT	-1.189	0.011008	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26517	GLDSCYAI			8	SLAY-screened peptide P4867			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCTCGACAGCTGCTACGCGATCTAGAGCAGCTGGTGCATTAACAATAGTTGGGAGAACTAA	GLDSCYAI*SSWCINNSWEN*	-1.189	0.000378	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26518	PLSTSMAHSISPACCITSHP			20	SLAY-screened peptide P4868			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTTAGCACGTCTATGGCCCATTCTATCTCGCCTGCCTGTTGCATTACTTCGCATCCGTAA	PLSTSMAHSISPACCITSHP*	-1.189	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26519	LTHNLALFHTHDAVLHQNKT			20	SLAY-screened peptide P4869			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCACCCATAACTTGGCCTTGTTCCATACGCATGACGCTGTCTTGCACCAGAATAAGACGTAA	LTHNLALFHTHDAVLHQNKT*	-1.189	0.006851	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26520	CVNTCVCGTVATPSLHKS			18	SLAY-screened peptide P4870			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGTGAACACGTGTGTTTGTGGCACTGTTGCTACGCCGAGTCTCCACAAGAGCTAGTATTAA	CVNTCVCGTVATPSLHKS*Y*	-1.189	0.03874	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26521	PTLIKFLAVFLHSNLCCSRY			20	SLAY-screened peptide P4871			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACGCTCATTAAGTTCCTTGCGGTCTTCTTGCATAGTAACCTGTGCTGTAGTCGCTACTAA	PTLIKFLAVFLHSNLCCSRY*	-1.188	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26522	RSHCGIPTHSIPWVLHTYSI			20	SLAY-screened peptide P4872			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTCCCACTGTGGTATTCCCACTCACAGTATTCCTTGGGTTCTTCATACTTACTCGATCTAA	RSHCGIPTHSIPWVLHTYSI*	-1.188	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26523	NSSIKPTDTSSYCYMPRLTP			20	SLAY-screened peptide P4873			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTCTAGCATTAAGCCTACTGATACGAGTAGCTATTGTTACATGCCTCGCCTGACCCCCTAA	NSSIKPTDTSSYCYMPRLTP*	-1.188	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26524	MNSLYNLSIIDGLHTKESSL			20	SLAY-screened peptide P4874			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGAATAGTTTGTATAACTTGTCTATCATTGATGGCTTGCACACCAAGGAGTCCTCCCTGTAA	MNSLYNLSIIDGLHTKESSL*	-1.188	0.003196	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26525	EASDFSAPSSNARFFTYHLF			20	SLAY-screened peptide P4875			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGCTTCTGATTTTAGCGCCCCCTCTAGCAATGCTCGGTTTTTTACTTACCACCTTTTCTAA	EASDFSAPSSNARFFTYHLF*	-1.188	0.001291	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26526	YPHCHTKPLYDLEVHTNNSY			20	SLAY-screened peptide P4876			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCCTCACTGCCATACTAAGCCCCTTTATGACCTGGAGGTGCATACCAATAATAGTTACTAA	YPHCHTKPLYDLEVHTNNSY*	-1.188	0.036753	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26527	VYSYSITIIGLVHIYILVVH			20	SLAY-screened peptide P4877			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTATTCTTACTCCATCACTATCATCGGGTTGGTCCACATCTACATTCTCGTCGTCCATTAA	VYSYSITIIGLVHIYILVVH*	-1.188	0.002534	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26528	GEPNSMHTYRRLGITCLHCL			20	SLAY-screened peptide P4878			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGAGCCGAACTCGATGCACACCTACCGTCGTCTTGGTATCACTTGCCTTCACTGTCTTTAA	GEPNSMHTYRRLGITCLHCL*	-1.188	0.001783	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26529	LAPVSANISTTTPFAL			16	SLAY-screened peptide P4879			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGCCCCCGTCTCCGCTAATATTTCGACGACCACCCCATTCGCCTTATGAAGCACGTCTAAC	LAPVSANISTTTPFAL*STSN	-1.187	0.005502	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26530	PCDSGSIYPFYHHRYSYVST			20	SLAY-screened peptide P4880			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGTGACTCGGGTTCTATTTACCCCTTCTACCATCATCGCTACTCGTACGTTAGCACGTAA	PCDSGSIYPFYHHRYSYVST*	-1.187	0.022832	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26531	ADTLIRRWTGRPDQFDCVIA			20	SLAY-screened peptide P4881			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGACACCCTTATTCGCCGTTGGACTGGCAGGCCTGATCAGTTCGACTGCGTGATTGCCTAA	ADTLIRRWTGRPDQFDCVIA*	-1.187	0.035187	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26532	SYWGALTGNINRTFIITYSP			20	SLAY-screened peptide P4882			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTATTGGGGCGCTCTTACCGGTAACATTAACCGCACCTTCATCATTACGTACAGTCCCTAA	SYWGALTGNINRTFIITYSP*	-1.187	0.002764	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26533	CGRIITSPFHPSWPLAIFAL			20	SLAY-screened peptide P4883			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGGCCGCATCATCACCAGCCCCTTTCACCCGAGCTGGCCCCTCGCGATCTTCGCTCTTTAA	CGRIITSPFHPSWPLAIFAL*	-1.187	0.023796	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26534	HARDPTDATSSRRTRLSNAF			20	SLAY-screened peptide P4884			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCCAGGGATCCCACTGACGCGACTTCTTCCAGGCGTACGCGGTTGTCCAACGCCTTTTAA	HARDPTDATSSRRTRLSNAF*	-1.187	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26535	IVMLFIFRLANSPSNNLLTH			20	SLAY-screened peptide P4885			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCGTCATGCTGTTTATTTTTCGGTTGGCTAATTCGCCTTCTAACAACCTGCTTACTCATTAA	IVMLFIFRLANSPSNNLLTH*	-1.186	2.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26536	RCAGRGAASTPLRRLVNWLL			20	SLAY-screened peptide P4886			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTGTGCCGGCCGCGGCGCCGCCTCTACGCCCCTGCGTAGGTTGGTTAATTGGCTCCTCTAA	RCAGRGAASTPLRRLVNWLL*	-1.186	0.001187	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26537	NAICNSEGYPVGLANMLTQC			20	SLAY-screened peptide P4887			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGCCATTTGTAACTCTGAGGGCTATCCCGTTGGCCTCGCTAATATGCTTACGCAGTGCTAA	NAICNSEGYPVGLANMLTQC*	-1.186	0.002457	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26538	ASANQDPVASEWSALPGYTI			20	SLAY-screened peptide P4888			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTCCGCCAACCAGGATCCGGTCGCTTCCGAGTGGTCCGCTTTGCCTGGGTACACCATCTAA	ASANQDPVASEWSALPGYTI*	-1.186	0.025128	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26539	CDAPYNNSPPGWNPFSMLVS			20	SLAY-screened peptide P4889			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGACGCGCCCTACAACAATAGTCCTCCTGGTTGGAATCCGTTTAGTATGCTTGTTTCTTAA	CDAPYNNSPPGWNPFSMLVS*	-1.186	0.001019	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26540	PFDWCRFSLETYLFCSMYRS			20	SLAY-screened peptide P4890			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTCGATTGGTGCCGCTTCAGCCTTGAGACGTATCTCTTCTGTTCCATGTACCGGTCGTAA	PFDWCRFSLETYLFCSMYRS*	-1.186	0.00022	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26541	ARNALGLFHNYRHQL			15	SLAY-screened peptide P4891			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCGTAACGCGTTGGGCCTCTTCCATAATTATCGCCATCAGCTCTAGCGGGCTAATTCTTAA	ARNALGLFHNYRHQL*RANS*	-1.186	0.02462	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26542	IDCKDFKFFTLALMIISLTLN			21	SLAY-screened peptide P4892			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGACTGTAAGGATTTTAAGTTCTTTACACTTGCTTTAATGATTATCTCTTTGACACTTAAC	IDCKDFKFFTLALMIISLTLN	-1.185	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26543	AQPTNHNRATGGPTHHDLDL			20	SLAY-screened peptide P4893			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCAGCCTACGAATCATAACCGTGCCACTGGCGGCCCGACGCACCACGACCTTGACCTCTAA	AQPTNHNRATGGPTHHDLDL*	-1.185	0.031291	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26544	WMSSPTNYSKVNIFRR			16	SLAY-screened peptide P4894			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGATGTCTAGCCCCACGAACTACAGTAAGGTTAACATTTTTCGTCGTTGACTCACCATTAAC	WMSSPTNYSKVNIFRR*LTIN	-1.185	0.043914	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26545	LQYAAPISPSNLWLTICPLM			20	SLAY-screened peptide P4895			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCAGTACGCTGCGCCTATTTCCCCTTCTAACCTTTGGTTGACTATTTGCCCCCTTATGTAA	LQYAAPISPSNLWLTICPLM*	-1.184	0.004279	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26546	NFVAFLPTCLPDFDFSMHYL			20	SLAY-screened peptide P4896			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTTTGTTGCTTTTCTTCCTACGTGTCTCCCTGACTTTGATTTTTCGATGCACTATCTCTAA	NFVAFLPTCLPDFDFSMHYL*	-1.184	3.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26547	RCGSINSDHLDYRASQVESL			20	SLAY-screened peptide P4897			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGTGTGGTTCTATTAATAGTGATCACCTTGACTATCGCGCGAGCCAGGTGGAGTCTCTGTAA	RCGSINSDHLDYRASQVESL*	-1.183	0.011878	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26548	SRTARALGPWLSGYSYPGCY			20	SLAY-screened peptide P4898			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCGCACTGCGCGCGCTCTTGGTCCGTGGCTTAGTGGCTACTCGTACCCGGGCTGTTATTAA	SRTARALGPWLSGYSYPGCY*	-1.183	0.001301	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26549	LVAFASSSMLLVLLDVQLT			19	SLAY-screened peptide P4899			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGGTTGCGTTTGCTAGTTCCTCCATGCTCCTTGTGCTTCTCGACGTTCAGCTTACGTAGTAA	LVAFASSSMLLVLLDVQLT**	-1.183	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26550	PNSGSHSVPHAWPTV			15	SLAY-screened peptide P4900			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAATAGCGGTTCTCATAGTGTTCCCCACGCCTGGCCCACTGTCTAGATCGACAGCTATTAA	PNSGSHSVPHAWPTV*IDSY*	-1.183	0.018992	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26551	CNVIKAICVCFTSDSVIHCY			20	SLAY-screened peptide P4901			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAACGTTATTAAGGCCATCTGCGTTTGCTTTACTAGCGACTCTGTTATTCATTGTTATTAA	CNVIKAICVCFTSDSVIHCY*	-1.183	0.026642	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26552	LVTAHLYPSIVPNIVG			16	SLAY-screened peptide P4902			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGGTCACCGCTCATCTTTATCCCTCTATCGTTCCCAACATTGTCGGCTAGTATTCCGATTAA	LVTAHLYPSIVPNIVG*YSD*	-1.183	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26553	PDALPSSHISYIICGPHSSS			20	SLAY-screened peptide P4903			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGACGCCCTTCCTAGTTCGCATATCTCTTATATTATTTGCGGCCCCCACTCCAGCAGTTAA	PDALPSSHISYIICGPHSSS*	-1.183	0.026844	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26554	LCVTYVAQNVPSLESNKFNS			20	SLAY-screened peptide P4904			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTGCGTCACCTACGTCGCCCAGAATGTCCCTTCCCTTGAGTCTAACAAGTTCAACAGTTAA	LCVTYVAQNVPSLESNKFNS*	-1.183	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26555	FISRTRLHLDTLGI			14	SLAY-screened peptide P4905			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCATTTCCCGTACTAGGCTTCATCTGGATACCCTGGGCATCTAGTATGCGCGTCTTTCGAAT	FISRTRLHLDTLGI*YARLSN	-1.182	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26556	TPLLWLWARTRPRFRKYIFI			20	SLAY-screened peptide P4906			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCCCCTTTTGTGGCTGTGGGCTAGGACCCGTCCGCGCTTCCGCAAGTACATTTTTATTTAA	TPLLWLWARTRPRFRKYIFI*	-1.182	0.025399	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26557	TKYSHCDPYDLHNLYSSFSA			20	SLAY-screened peptide P4907			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAAGTACTCCCACTGTGACCCCTATGATCTTCATAATCTGTACTCTTCCTTTTCTGCTTAA	TKYSHCDPYDLHNLYSSFSA*	-1.182	0.003417	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26558	WDSDFPQYILLL			12	SLAY-screened peptide P4908			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGGATTCTGATTTTCCGCAGTACATTCTTCTCCTCTAGGAGACCGCTTTCCATGCTTCTTAA	WDSDFPQYILLL*ETAFHAS*	-1.182	0.001962	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26559	HCPPPFYMLESKTGRYNHYV			20	SLAY-screened peptide P4909			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGCCCCCCCCCCTTCTATATGCTCGAGTCTAAGACGGGCCGCTATAACCACTACGTTTAA	HCPPPFYMLESKTGRYNHYV*	-1.182	0.028625	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26560	ICISPWDDTVYIPGVRMWHL			20	SLAY-screened peptide P4910			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTTGTATCTCCCCCTGGGACGATACTGTCTATATTCCGGGTGTCCGGATGTGGCACCTCTAA	ICISPWDDTVYIPGVRMWHL*	-1.181	0.000386	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26561	STCCQFTNGCGWHYIQAATS			20	SLAY-screened peptide P4911			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCACTTGCTGTCAGTTTACGAACGGCTGCGGGTGGCACTATATCCAGGCCGCTACCTCCTAA	STCCQFTNGCGWHYIQAATS*	-1.181	0.016623	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26562	HAGFSLLYTWSAWRGRTYTL			20	SLAY-screened peptide P4912			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCTGGCTTCTCCCTCCTCTACACTTGGAGCGCGTGGCGCGGTCGTACTTACACGCTGTAA	HAGFSLLYTWSAWRGRTYTL*	-1.181	4.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26563	PPSNFNRWPATCVIVSGMHY			20	SLAY-screened peptide P4913			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCCAGTAACTTCAACCGTTGGCCCGCTACTTGTGTTATTGTTAGTGGCATGCATTATTAA	PPSNFNRWPATCVIVSGMHY*	-1.181	0.030921	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26564	TAGLSSANTCVPPCLRLSLPL			21	SLAY-screened peptide P4914			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGCTGGGCTCAGTAGCGCCAACACGTGCGTCCCGCCCTGCTTACGATTGTCGCTCCCCTTA	TAGLSSANTCVPPCLRLSLPL	-1.18	0.004067	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26565	TCFGSLLYLANSPAPE			16	SLAY-screened peptide P4915			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTGTTTTGGGAGTCTGCTGTACCTGGCTAATAGCCCGGCCCCCGAGTAGTTTCACTTTTAA	TCFGSLLYLANSPAPE*FHF*	-1.18	0.000164	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26566	ACVASLVLTVDHTRLSVLFA			20	SLAY-screened peptide P4916			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTGTGTTGCTAGCCTCGTCCTCACCGTTGACCATACTCGCCTCTCCGTTCTTTTCGCGTAA	ACVASLVLTVDHTRLSVLFA*	-1.18	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26567	CPGRLHTCFDLWYPMVLIAFN			21	SLAY-screened peptide P4917			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCCGGTCGGCTCCATACTTGTTTCGATCTCTGGTATCCAATGGTTCTAATTGCCTTTAAC	CPGRLHTCFDLWYPMVLIAFN	-1.18	0.001556	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26568	IIGTVCNCLSYYWAYCLYTL			20	SLAY-screened peptide P4918			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCATTGGTACGGTGTGCAATTGCTTGTCGTACTATTGGGCTTACTGCTTGTACACCCTTTAA	IIGTVCNCLSYYWAYCLYTL*	-1.18	0.002485	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26569	SSNHGNACFCNWHHNIINCF			20	SLAY-screened peptide P4919			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTCCAACCATGGCAACGCTTGCTTTTGCAACTGGCATCACAATATTATCAATTGTTTTTAA	SSNHGNACFCNWHHNIINCF*	-1.18	0.004466	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26570	NNPNVHTRCDIPVPAFVLA			19	SLAY-screened peptide P4920			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACAACCCTAACGTCCACACGAGGTGTGATATTCCCGTTCCCGCCTTTGTTCTTGCGTAACTG	NNPNVHTRCDIPVPAFVLA*L	-1.179	0.013512	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26571	NDLLGVSFSYAII			13	SLAY-screened peptide P4921			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGATCTCCTGGGTGTTAGTTTCAGCTATGCCATTATCTAGTGCCCGGATTACAAGTAACTG	NDLLGVSFSYAII*CPDYK*L	-1.179	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26572	PHPDRYYANITFVSREFPSA			20	SLAY-screened peptide P4922			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCATCCCGACCGCTACTACGCTAACATCACCTTTGTTTCTCGCGAGTTTCCTTCCGCGTAA	PHPDRYYANITFVSREFPSA*	-1.178	0.001477	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26573	DDWSLPRSLVFRWSRDSIPN			20	SLAY-screened peptide P4923			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATGACTGGTCGCTTCCTCGGTCCCTGGTTTTTCGTTGGTCCAGGGACAGTATCCCTAATTAA	DDWSLPRSLVFRWSRDSIPN*	-1.178	0.00338	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26574	PPTAKTSPSNYAPYK			15	SLAY-screened peptide P4924			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCTACTGCCAAGACTTCCCCGTCGAATTACGCGCCCTATAAGTAGTTGTACGATGAGTAA	PPTAKTSPSNYAPYK*LYDE*	-1.178	0.001918	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26575	CHYDIFHNCSMDYSIFLKYL			20	SLAY-screened peptide P4925			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCACTATGACATTTTCCATAATTGCTCCATGGATTATAGCATTTTCCTTAAGTACCTCTAA	CHYDIFHNCSMDYSIFLKYL*	-1.178	0.000157	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26576	PRHPDTKKYSDSYTLCPLWLL			21	SLAY-screened peptide P4926			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGCCACCCGGACACTAAGAAGTACAGCGATAGTTATACGCTTTGTCCTTTGTGGCTATTA	PRHPDTKKYSDSYTLCPLWLL	-1.178	0.035818	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26577	PNTFFRANSHLIDICRDAPT			20	SLAY-screened peptide P4927			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAATACGTTTTTCCGGGCTAATTCCCACCTGATCGACATTTGCCGTGATGCTCCGACGTAA	PNTFFRANSHLIDICRDAPT*	-1.177	8.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26578	ARLHSKVREHSLSSAC			16	SLAY-screened peptide P4928			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCGGCTCCACAGTAAGGTTCGCGAGCACTCGCTTTCTAGCGCCTGTTAGTGTCTCAATTAA	ARLHSKVREHSLSSAC*CLN*	-1.177	0.022829	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26579	SRSFCSPRVRPPLLVAPLYM			20	SLAY-screened peptide P4929			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCGGTCCTTCTGCTCCCCCCGGGTCCGCCCCCCCTTGCTTGTCGCCCCGCTCTATATGTAA	SRSFCSPRVRPPLLVAPLYM*	-1.177	0.001475	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26580	CRDSTGPATGSPTYIIPVHI			20	SLAY-screened peptide P4930			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGTGATAGCACGGGTCCGGCCACTGGGTCCCCCACTTACATTATCCCCGTCCATATTTAA	CRDSTGPATGSPTYIIPVHI*	-1.177	0.0001	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26581	HLRHSVTTGDTRNHNGHSLRN			21	SLAY-screened peptide P4931			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCTTCGCCACTCGGTTACCACCGGTGACACCCGGAACCATAACGGCCATTCCCTCCGTAAC	HLRHSVTTGDTRNHNGHSLRN	-1.177	0.00123	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26582	TDETHLALYQSTNEIPNIRN			20	SLAY-screened peptide P4932			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGACGAGACTCATTTGGCCCTCTACCAGAGTACCAACGAGATTCCGAATATTAGGAATTAA	TDETHLALYQSTNEIPNIRN*	-1.177	0.012115	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26583	SGLTNLSTTYYRLRCIHLNN			20	SLAY-screened peptide P4933			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGGCCTGACTAATCTCTCTACGACGTACTATCGTTTGCGCTGTATCCACCTTAATAACTAA	SGLTNLSTTYYRLRCIHLNN*	-1.177	0.012233	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26584	PIAAKPDTRFDNCVLLHHSV			20	SLAY-screened peptide P4934			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATTGCTGCCAAGCCTGATACGCGGTTCGATAATTGTGTGCTCTTGCACCATTCGGTCTAA	PIAAKPDTRFDNCVLLHHSV*	-1.177	0.00061	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26585	LNSDHYTLEDPMLPFVSPRR			20	SLAY-screened peptide P4935			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGAACTCCGACCACTATACCCTTGAGGACCCCATGCTTCCCTTTGTTTCGCCGCGTAGGTAA	LNSDHYTLEDPMLPFVSPRR*	-1.177	0.003818	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26586	RYRFPAPVYSSSNHCYTYGY			20	SLAY-screened peptide P4936			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTATAGGTTTCCCGCGCCTGTTTATTCTAGTAGTAATCACTGTTACACGTATGGTTACTAA	RYRFPAPVYSSSNHCYTYGY*	-1.177	0.015201	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26587	ARDLPPAFASGYGGRCLYLW			20	SLAY-screened peptide P4937			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCGCGATCTTCCCCCGGCCTTCGCCTCGGGGTACGGTGGTCGTTGTCTTTACCTTTGGTAA	ARDLPPAFASGYGGRCLYLW*	-1.177	0.000224	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26588	TTNIIKKLVSACLKRASLF			19	SLAY-screened peptide P4938			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTACCAACATTATCAAGAAACTCGTTTCAGCATGTCTCAAGCGCGCCTCCCTGTTCTAACTG	TTNIIKKLVSACLKRASLF*L	-1.177	1.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26589	TVGIFNAATDFGHPTLTPFM			20	SLAY-screened peptide P4939			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTTGGGATCTTCAATGCTGCTACCGATTTCGGGCATCCGACGCTCACTCCGTTTATGTAA	TVGIFNAATDFGHPTLTPFM*	-1.175	0.007466	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26590	ADPLTSCDFYLLQYASL			17	SLAY-screened peptide P4940			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGACCCCCTGACCTCTTGCGATTTCTACCTTTTGCAGTACGCCAGTTTGTAGCGCGTCTAA	ADPLTSCDFYLLQYASL*RV*	-1.175	0.008808	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26591	VIMRSHCSTNISRNTSNHTY			20	SLAY-screened peptide P4941			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGATTATGCGGAGCCACTGTAGTACCAACATTAGCCGCAATACCAGTAACCATACTTATTAA	VIMRSHCSTNISRNTSNHTY*	-1.174	0.004909	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26592	GLNLPTCGKLGVTPVITVIL			20	SLAY-screened peptide P4942			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCTGAACCTTCCCACTTGTGGGAAGCTCGGTGTCACGCCCGTTATTACGGTTATTCTCTAA	GLNLPTCGKLGVTPVITVIL*	-1.173	0.000808	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26593	MNLASPRFILIPMC			14	SLAY-screened peptide P4943			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGAACCTTGCAAGCCCACGATTCATTCTGATACCGATGTGTTGACCCCTAGTATGTAACTGA	MNLASPRFILIPMC*PLVCN*	-1.173	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26594	EQCEVPQTNYSLQTNSWVEL			20	SLAY-screened peptide P4944			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCAGTGTGAGGTCCCCCAGACGAACTATTCCCTCCAGACCAATTCCTGGGTTGAGCTCTAA	EQCEVPQTNYSLQTNSWVEL*	-1.173	0.004297	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26595	FFCPTLYRSPLDRLSIVHPS			20	SLAY-screened peptide P4945			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTTTTGTCCGACTCTCTACAGGAGCCCTCTCGACCGCTTGTCTATTGTTCACCCCTCTTAA	FFCPTLYRSPLDRLSIVHPS*	-1.173	0.04991	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26596	HCYCYPHHFHDNPFLRLICF			20	SLAY-screened peptide P4946			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGCTACTGCTACCCTCATCATTTCCATGACAATCCGTTCCTTAGGCTGATTTGTTTTTAA	HCYCYPHHFHDNPFLRLICF*	-1.172	0.000131	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26597	HPNDRHIHD			9	SLAY-screened peptide P4947			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCCAATGACCGCCATATTCATGACTAGCACCGCGCCAAGCAGCACTGCCACCGCCCGTAA	HPNDRHIHD*HRAKQHCHRP*	-1.172	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26598	IHCLMSSA			8	SLAY-screened peptide P4948			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCATTGCTTGATGAGTTCTGCCTGATGCCTGTCAGGACGTGCAACAACAGGCCGAAGTAAC	IHCLMSSA*CLSGRATTGRSN	-1.172	0.01329	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26599	CPGNANYSFCNWHCWTPLFG			20	SLAY-screened peptide P4949			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCTGGGAATGCTAACTATAGCTTTTGCAACTGGCATTGTTGGACGCCGCTCTTTGGTTAA	CPGNANYSFCNWHCWTPLFG*	-1.172	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26600	CSLFFPTIYHMA			12	SLAY-screened peptide P4950			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTCGCTTTTCTTCCCCACTATCTACCATATGGCTTAGTTTGGGCCCTTTCACTGGATCTAA	CSLFFPTIYHMA*FGPFHWI*	-1.172	0.01406	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26601	LKPYRILSNWIAGLTTLPFN			20	SLAY-screened peptide P4951			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGAAGCCCTATCGCATTCTCTCCAACTGGATTGCGGGGCTGACCACGTTGCCCTTTAACTAA	LKPYRILSNWIAGLTTLPFN*	-1.172	0.008599	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26602	LKRNHTTRQKHYGLLPCWNT			20	SLAY-screened peptide P4952			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAAGCGGAATCACACCACCCGCCAGAAGCACTACGGCCTTCTTCCTTGCTGGAATACCTAA	LKRNHTTRQKHYGLLPCWNT*	-1.171	0.001675	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26603	TTNSYDFIITSCSVPARHNP			20	SLAY-screened peptide P4953			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGACCAATTCCTATGACTTCATTATCACTTCGTGTTCGGTCCCCGCCCGGCACAATCCCTAA	TTNSYDFIITSCSVPARHNP*	-1.171	0.025223	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26604	GSCALLGEFPN			11	SLAY-screened peptide P4954			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTCGTGTGCTCTGCTTGGGGAGTTTCCGAACTAGTTGCATCCGACTCATACGTAGCATTAA	GSCALLGEFPN*LHPTHT*H*	-1.171	0.03453	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26605	PARPNTTHNIVSSATSWLST			20	SLAY-screened peptide P4955			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCGCGTCCTAACACCACGCACAATATCGTGTCTTCGGCTACGAGTTGGTTGAGTACTTAA	PARPNTTHNIVSSATSWLST*	-1.171	0.000422	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26606	QSPYLIPMHLSIYYSNVKPP			20	SLAY-screened peptide P4956			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGAGCCCTTATCTTATCCCGATGCACTTGAGCATTTATTATTCCAATGTTAAGCCGCCGTAA	QSPYLIPMHLSIYYSNVKPP*	-1.171	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26607	AAMNPNRTPLGAPSCGDLTF			20	SLAY-screened peptide P4957			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGCTATGAACCCGAATCGCACCCCGCTCGGGGCCCCTTCCTGCGGTGATCTCACTTTCTAA	AAMNPNRTPLGAPSCGDLTF*	-1.17	0.000125	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26608	PYCLAVS			7	SLAY-screened peptide P4958			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTACTGCCTTGCCGTTTCTTAGAGTTGCGTGACGGTTCTCATTTCCGCGCATGTGTGGTAA	PYCLAVS*SCVTVLISAHVW*	-1.17	0.012656	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26609	YHCWSPVYHSFQDPYNLSTH			20	SLAY-screened peptide P4959			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCACTGTTGGAGTCCGGTCTACCATAGTTTTCAGGATCCCTATAATTTGAGTACTCATTAA	YHCWSPVYHSFQDPYNLSTH*	-1.17	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26610	RDATITYKVMLIFIIIAVRRN			21	SLAY-screened peptide P4960			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGATGCCACGATTACCTATAAAGTCATGCTGATTTTTATTATTATTGCCGTAAGGCGTAAC	RDATITYKVMLIFIIIAVRRN	-1.17	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26611	LRFYSLTDKNYSEINCTIPS			20	SLAY-screened peptide P4961			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCGGTTTTATTCCCTCACTGATAAGAATTATAGCGAGATCAACTGCACTATTCCGTCCTAA	LRFYSLTDKNYSEINCTIPS*	-1.17	0.00171	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26612	AFYKRLMFHRCLMRIRVIRQ			20	SLAY-screened peptide P4962			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTTTTACAAGCGCCTTATGTTTCATCGTTGTCTGATGAGGATCAGGGTCATTCGTCAGTAA	AFYKRLMFHRCLMRIRVIRQ*	-1.169	0.014128	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26613	RHRVTLFLALTTKSMTGFSR			20	SLAY-screened peptide P4963			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCACCGCGTGACTCTCTTCCTTGCGCTGACTACTAAGAGTATGACGGGTTTCTCGCGTTAA	RHRVTLFLALTTKSMTGFSR*	-1.169	0.000157	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26614	PSRCHHPVLLYTASLRNFSD			20	SLAY-screened peptide P4964			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTCCCGGTGCCACCATCCTGTGCTCCTGTACACGGCCTCGCTGCGCAACTTCTCGGATTAA	PSRCHHPVLLYTASLRNFSD*	-1.169	0.000304	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26615	HPTCSCRIPSSFPPPAVSVT			20	SLAY-screened peptide P4965			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCCGACGTGCTCCTGCCGTATTCCTTCCTCTTTTCCGCCTCCGGCCGTGAGCGTTACGTAA	HPTCSCRIPSSFPPPAVSVT*	-1.169	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26616	LLST			4	SLAY-screened peptide P4966			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTTGTCTACCTAGTTCCTTCACGATCTTACGAATTATTGGCACTAGAGGACGTCCATGTAA	LLST*FLHDLTNYWH*RTSM*	-1.169	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26617	AFLTGPDSIDPHNLPAYVSY			20	SLAY-screened peptide P4967			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTTCCTGACCGGGCCGGACAGCATTGACCCCCACAATCTCCCGGCTTATGTCTCGTACTAA	AFLTGPDSIDPHNLPAYVSY*	-1.169	0.002997	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26618	ATSSDYHDGTALKSINHSQI			20	SLAY-screened peptide P4968			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGACCAGTAGTGATTATCATGACGGTACGGCTTTGAAGTCTATCAACCACTCCCAGATTTAA	ATSSDYHDGTALKSINHSQI*	-1.169	0.001551	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26619	CLLNWPQNHCRFCGSHQDWT			20	SLAY-screened peptide P4969			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTCCTGAATTGGCCCCAGAACCACTGCCGCTTCTGTGGCAGCCATCAGGATTGGACTTAA	CLLNWPQNHCRFCGSHQDWT*	-1.169	0.000759	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26620	RESCWRFIKLDNALYYRSKTE			21	SLAY-screened peptide P4970			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGGAGTCCTGCTGGCGTTTCATCAAGCTCGATAATGCCTTGTATTACCGTTCCAAGACTGAG	RESCWRFIKLDNALYYRSKTE	-1.168	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26621	NASCPPSCLNHHRGLPK			17	SLAY-screened peptide P4971			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACGCCAGCTGTCCCCCCTCCTGCCTTAATCACCACCGTGGTCTTCCCAAGTAGACGAAGTAA	NASCPPSCLNHHRGLPK*TK*	-1.168	0.016649	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26622	ACPRARVIREALLGLIPDER			20	SLAY-screened peptide P4972			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTGCCCCCGCGCGCGGGTTATTCGTGAGGCCCTTCTCGGCCTGATTCCGGACGAGCGCTAA	ACPRARVIREALLGLIPDER*	-1.168	0.007268	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26623	PRVIHTPSYNSRHSDNLLST			20	SLAY-screened peptide P4973			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGTGTCATTCATACTCCCTCTTACAACTCTAGGCATAGTGATAATTTGCTGAGTACCTAA	PRVIHTPSYNSRHSDNLLST*	-1.168	3.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26624	MLFTRPFLYYRAYAYPTYMV			20	SLAY-screened peptide P4974			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCTGTTTACCCGTCCGTTTCTGTACTATCGCGCGTATGCCTATCCCACCTATATGGTCTAA	MLFTRPFLYYRAYAYPTYMV*	-1.168	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26625	SIGHSCPKQMDNQGLLLAWT			20	SLAY-screened peptide P4975			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCATTGGCCATAGTTGTCCTAAGCAGATGGATAATCAGGGGCTCCTCTTGGCGTGGACCTAA	SIGHSCPKQMDNQGLLLAWT*	-1.168	4.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26626	RRPFSSVLIILLLLRGVMMGN			21	SLAY-screened peptide P4976			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGCCCCTTTAGTTCAGTTCTCATCATTTTGCTGTTATTGCGAGGCGTCATGATGGGTAAC	RRPFSSVLIILLLLRGVMMGN	-1.168	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26627	PCSPHFPDYPNMVSKLLLQA			20	SLAY-screened peptide P4977			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGCTCGCCTCACTTTCCGGATTATCCGAACATGGTTTCGAAGCTCCTGCTCCAGGCCTAA	PCSPHFPDYPNMVSKLLLQA*	-1.167	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26628	EQENEYDHGTYKGPIPQFFF			20	SLAY-screened peptide P4978			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCAGGAGAATGAGTATGACCATGGGACGTACAAGGGTCCTATTCCCCAGTTTTTTTTCTAA	EQENEYDHGTYKGPIPQFFF*	-1.167	0.000102	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26629	RLSACINPFCISSDTDF			17	SLAY-screened peptide P4979			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCTTAGCGCGTGCATTAACCCCTTTTGCATCTCTAGCGATACCGACTTCTAGCGTCCCTAA	RLSACINPFCISSDTDF*RP*	-1.167	0.030602	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26630	APRNAKLFIYWKSTSEDVSCL			21	SLAY-screened peptide P4980			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCCTCGTAACGCGAAGCTTTTCATCTACTGGAAGTCCACTTCCGAGGACGTTAGTTGCTTA	APRNAKLFIYWKSTSEDVSCL	-1.167	0.014175	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26631	WRYMINPRALLCKMCKRACI			20	SLAY-screened peptide P4981			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCGCTATATGATTAATCCGCGGGCCTTGCTTTGCAAGATGTGTAAGCGTGCCTGCATCTAA	WRYMINPRALLCKMCKRACI*	-1.167	0.001449	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26632	YYLNCTPFMLQSTQIMKGTY			20	SLAY-screened peptide P4982			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTACCTGAATTGCACGCCGTTTATGCTCCAGAGTACGCAGATCATGAAGGGCACTTATTAA	YYLNCTPFMLQSTQIMKGTY*	-1.166	0.000167	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26633	NLKSHDYVCFPYAVLQSYNN			20	SLAY-screened peptide P4983			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCTTAAGTCTCATGATTACGTCTGCTTTCCGTACGCTGTTTTGCAGTCTTATAACAACTAA	NLKSHDYVCFPYAVLQSYNN*	-1.166	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26634	HRTLRPYPSDPIICRHILHF			20	SLAY-screened peptide P4984			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCGTACCCTCCGGCCCTACCCCTCTGACCCCATCATCTGCAGGCACATCCTGCACTTCTAA	HRTLRPYPSDPIICRHILHF*	-1.166	0.012787	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26635	YCCCLTPHLAAKEQYVLTFF			20	SLAY-screened peptide P4985			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTGCTGCTGTCTTACCCCCCATCTCGCTGCCAAGGAGCAGTACGTTTTGACCTTCTTTTAA	YCCCLTPHLAAKEQYVLTFF*	-1.166	0.028447	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26636	LRGSLYPAPPTWAWFTPHKT			20	SLAY-screened peptide P4986			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAGGGGGTCGCTTTATCCGGCTCCTCCCACTTGGGCCTGGTTTACCCCTCATAAGACTTAA	LRGSLYPAPPTWAWFTPHKT*	-1.166	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26637	AQCTPFCNNYDISTNSVTVT			20	SLAY-screened peptide P4987			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCAGTGCACCCCCTTCTGTAATAACTATGACATTTCTACCAATAGTGTTACCGTTACCTAA	AQCTPFCNNYDISTNSVTVT*	-1.166	0.030683	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26638	AGPLNFCPGLYVPYAGPDLM			20	SLAY-screened peptide P4988			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGGCCCCCTCAATTTTTGCCCCGGCCTTTATGTTCCTTACGCCGGGCCCGATCTTATGTAA	AGPLNFCPGLYVPYAGPDLM*	-1.166	0.000496	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26639	MSLIPTQWMISLIFFARYRT			20	SLAY-screened peptide P4989			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTCTTTGATTCCTACTCAGTGGATGATCTCTTTGATCTTTTTCGCCAGGTACCGTACGTAA	MSLIPTQWMISLIFFARYRT*	-1.166	0.003087	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26640	LTNFAPGQTLMYRDSTDTKN			20	SLAY-screened peptide P4990			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGACTAATTTTGCGCCCGGGCAGACGTTGATGTACAGGGATTCCACTGATACCAAGAATTAA	LTNFAPGQTLMYRDSTDTKN*	-1.166	0.007044	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26641	VPHPVSKRVTSLYKLEVTLY			20	SLAY-screened peptide P4991			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCCCCACCCTGTGTCTAAGAGGGTCACCTCCCTGTATAAGCTCGAGGTCACGCTGTACTAA	VPHPVSKRVTSLYKLEVTLY*	-1.166	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26642	FDNLGACPVLWGLTLLS			17	SLAY-screened peptide P4992			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGATAATCTCGGTGCCTGCCCTGTTCTGTGGGGCCTCACTCTTCTCAGTTAGACCCCTTAA	FDNLGACPVLWGLTLLS*TP*	-1.165	0.029734	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26643	TSSNAPCIHDFHALCNMRPN			20	SLAY-screened peptide P4993			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCTAGTAACGCCCCTTGTATCCATGACTTCCACGCCCTTTGCAACATGCGGCCTAACTAA	TSSNAPCIHDFHALCNMRPN*	-1.165	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26644	WSNTDHDDHSSLNLIVLYEQ			20	SLAY-screened peptide P4994			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTCCAATACGGACCATGATGATCACTCCAGTTTGAACCTGATCGTCCTCTATGAGCAGTAA	WSNTDHDDHSSLNLIVLYEQ*	-1.165	0.013518	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26645	HSTYIHPYFCRPLYKLEHSP			20	SLAY-screened peptide P4995			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTCCACCTATATCCACCCCTATTTTTGCCGGCCGCTCTATAAGCTCGAGCATTCGCCTTAA	HSTYIHPYFCRPLYKLEHSP*	-1.165	0.015681	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26646	GDTYKHSTSLLLNHGSSNCML			21	SLAY-screened peptide P4996			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTGACACCTACAAGCATAGTACCAGTTTGCTGCTTAATCACGGTTCCTCTAACTGCATGTTA	GDTYKHSTSLLLNHGSSNCML	-1.164	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26647	SRTIRPCPRPCAIKIHYALE			20	SLAY-screened peptide P4997			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCGTACCATCCGTCCCTGCCCTCGCCCTTGTGCGATCAAGATCCACTATGCCTTGGAGTAA	SRTIRPCPRPCAIKIHYALE*	-1.164	0.049403	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26648	AHYTLLPRNTGCAPQHNAWE			20	SLAY-screened peptide P4998			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCACTACACGCTCTTGCCCCGTAATACGGGTTGCGCGCCCCAGCATAACGCGTGGGAGTAA	AHYTLLPRNTGCAPQHNAWE*	-1.164	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26649	ASDRP			5	SLAY-screened peptide P4999			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTCGGACCGACCATGACTCCTTGCAATTCTTCTCTTCGCGACCTTCACCATAACAGTTAAC	ASDRP*LLAILLFATFTITVN	-1.164	0.002613	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26650	LSTTTFLCLARRPRKYEENG			20	SLAY-screened peptide P5000			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTCCACTACGACCTTTTTGTGCCTTGCCAGGCGGCCGAGGAAGTACGAGGAGAATGGCTAA	LSTTTFLCLARRPRKYEENG*	-1.164	0.006072	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26651	SPPRARPRDPDTTSWGNNLT			20	SLAY-screened peptide P5001			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCCGCCCCGCGCTCGCCCCCGCGATCCCGACACTACCAGCTGGGGCAACAACCTTACCTAA	SPPRARPRDPDTTSWGNNLT*	-1.164	0.033822	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26652	DNPAYRHQLHYAPPPTDSAH			20	SLAY-screened peptide P5002			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACAATCCTGCTTATCGCCACCAGCTCCACTATGCCCCTCCGCCCACGGATTCCGCGCACTAA	DNPAYRHQLHYAPPPTDSAH*	-1.163	0.028351	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26653	LKSGKPTCDVDNFYLITFRP			20	SLAY-screened peptide P5003			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAAGTCCGGCAAGCCCACGTGCGACGTCGACAACTTCTACCTCATCACCTTTCGTCCCTAA	LKSGKPTCDVDNFYLITFRP*	-1.163	0.001394	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26654	HPHSNNHVD			9	SLAY-screened peptide P5004			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCTCACTCCAATAACCACGTTGATTAGTGCTATCCGGTCTGCAACAAGAACCTTGATTAA	HPHSNNHVD*CYPVCNKNLD*	-1.163	0.001304	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26655	YAGKNYMYRLPRLQRSCGIN			20	SLAY-screened peptide P5005			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGCGGGCAAGAATTACATGTATCGTCTCCCTCGCCTCCAGCGGTCTTGTGGGATTAATTAA	YAGKNYMYRLPRLQRSCGIN*	-1.162	0.026815	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26656	CAHLNCTITVVHDNS			15	SLAY-screened peptide P5006			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGCTCATCTCAACTGCACTATCACGGTTGTCCATGATAATAGTTAGGACGAGATTTTTTAA	CAHLNCTITVVHDNS*DEIF*	-1.162	0.002414	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26657	SCPGPHQFPLIHINTTYRTF			20	SLAY-screened peptide P5007			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTGCCCGGGCCCGCACCAGTTTCCGCTTATTCATATTAACACGACGTACCGGACCTTTTAA	SCPGPHQFPLIHINTTYRTF*	-1.162	0.031643	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26658	CLHFLS			6	SLAY-screened peptide P5008			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTTGCACTTTCTCTCCTAGAACTTTACGGATAGTCAGTAGGGTCCGACGCGTAATGGGTAA	CLHFLS*NFTDSQ*GPTRNG*	-1.162	0.005521	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26659	YDDCDDITDRREKFYLIFIH			20	SLAY-screened peptide P5009			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATGATGATTGTGACGATATCACCGATCGGCGCGAGAAGTTTTATCTTATTTTTATCCATTAA	YDDCDDITDRREKFYLIFIH*	-1.162	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26660	GSNDYKHSSSSISRMTCTLD			20	SLAY-screened peptide P5010			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTCGAACGACTATAAGCACTCTTCGTCCTCCATCAGTAGGATGACTTGTACTCTCGATTAA	GSNDYKHSSSSISRMTCTLD*	-1.162	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26661	RPPAPLCPWLRHPHSGPITV			20	SLAY-screened peptide P5011			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCGCCGGCGCCTCTCTGCCCCTGGCTCCGGCATCCCCACTCTGGCCCTATTACTGTTTAA	RPPAPLCPWLRHPHSGPITV*	-1.162	0.004672	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26662	YRYLPLYFWGCPLYHTSTYV			20	SLAY-screened peptide P5012			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATAGGTATCTCCCGCTTTACTTTTGGGGTTGTCCGCTGTATCACACCAGTACCTACGTGTAA	YRYLPLYFWGCPLYHTSTYV*	-1.161	2.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26663	TALCVHLTSHTTFRTPYNLP			20	SLAY-screened peptide P5013			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGCGTTGTGCGTGCACCTCACGTCGCACACGACCTTCCGCACCCCGTACAATCTTCCTTAA	TALCVHLTSHTTFRTPYNLP*	-1.161	0.041654	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26664	EGLWSFQCYPRFIFSLHDYNY			21	SLAY-screened peptide P5014			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGGGCTCTGGAGCTTTCAGTGTTACCCCAGGTTCATCTTTTCCTTGCACGATTATAACTAC	EGLWSFQCYPRFIFSLHDYNY	-1.161	0.017235	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26665	QCQYCHTNTENHQSAYR			17	SLAY-screened peptide P5015			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTGTCAGTACTGCCATACTAATACCGAGAATCACCAGTCTGCGTATAGGTAGTAGTTGTAA	QCQYCHTNTENHQSAYR**L*	-1.161	0.013663	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26666	YPLRPRIYSCRFCIFLRI			18	SLAY-screened peptide P5016			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCCTCTTCGTCCCCGGATTTATTCTTGTCGTTTCTGTATCTTTTTGAGGATTTAGCATTAA	YPLRPRIYSCRFCIFLRI*H*	-1.161	0.000288	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26667	PFQPDLNNYQKIFFCNLIAP			20	SLAY-screened peptide P5017			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTCCAGCCCGACCTGAACAATTATCAGAAGATTTTCTTTTGCAACTTGATTGCCCCTTAA	PFQPDLNNYQKIFFCNLIAP*	-1.161	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26668	HHKC			4	SLAY-screened peptide P5018			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCATAAGTGTTAGGTGTCCTCTACGACGAACTACTCCAACGTTAATAACGTCATTCAGTAA	HHKC*VSSTTNYSNVNNVIQ*	-1.16	0.019447	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26669	SYCCCSFPRNNDFSTADELI			20	SLAY-screened peptide P5019			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTACTGCTGTTGCAGCTTTCCCCGCAATAACGACTTCAGCACCGCCGACGAGCTCATCTAA	SYCCCSFPRNNDFSTADELI*	-1.16	0.004704	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26670	NTNY			4	SLAY-screened peptide P5020			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACACTAACTATTAACCTGCCTGTTACACGTGGCATACCAATGATGGCCCCTCCTGGGATTAA	NTNY*PACYTWHTNDGPSWD*	-1.16	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26671	GRRIQPHSLSPCTATASV			18	SLAY-screened peptide P5021			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCGTCGCATCCAGCCGCATAGCCTGAGTCCGTGTACGGCTACTGCTTCCGTGTAACTGAGT	GRRIQPHSLSPCTATASV*LS	-1.16	0.000126	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26672	EAQARKRPRPLWRFPRVGDE			20	SLAY-screened peptide P5022			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGCTCAGGCTCGCAAGAGGCCCCGTCCCCTTTGGCGTTTCCCGCGCGTTGGCGACGAGTAA	EAQARKRPRPLWRFPRVGDE*	-1.16	0.000946	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26673	NGNFKRYRNICGNLSINHLC			20	SLAY-screened peptide P5023			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGGCAATTTTAAGAGGTACCGTAATATCTGCGGCAACTTGTCCATCAATCACTTGTGTTAA	NGNFKRYRNICGNLSINHLC*	-1.16	2.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26674	LGGQLCTTSIHVSYNLCCDM			20	SLAY-screened peptide P5024			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGGTGGGCAGCTTTGCACTACCTCCATTCATGTGTCTTATAACCTTTGTTGCGATATGTAA	LGGQLCTTSIHVSYNLCCDM*	-1.16	0.007633	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26675	LCARLRFLCLTLGTLHRKAV			20	SLAY-screened peptide P5025			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTGCGCGCGCCTTCGGTTCCTTTGTCTCACTCTTGGTACTCTTCACAGGAAGGCTGTGTAA	LCARLRFLCLTLGTLHRKAV*	-1.159	0.000517	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26676	VKNFFRMTRRRCSSIVSSLLN			21	SLAY-screened peptide P5026			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTAAGAATTTTTTTCGAATGACTAGAAGGCGATGTTCAAGTATAGTCTCATCTCTTCTTAAC	VKNFFRMTRRRCSSIVSSLLN	-1.159	0.006193	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26677	LFNILLYNTQTLPRSHVP			18	SLAY-screened peptide P5027			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTTAATATCTTGCTTTATAATACGCAGACTCTTCCCCGTTCCCATGTTCCTTAGCACTAA	LFNILLYNTQTLPRSHVP*H*	-1.159	1.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26678	CWYPTLPSSLYQYSLQVAMS			20	SLAY-screened peptide P5028			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTGGTATCCCACGCTGCCCTCGAGCCTTTACCAGTACAGCCTTCAGGTCGCCATGTCCTAA	CWYPTLPSSLYQYSLQVAMS*	-1.159	0.003555	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26679	HTTNRYHYICLWFSCFTFNV			20	SLAY-screened peptide P5029			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACTACCAATCGGTATCACTACATTTGCCTTTGGTTTTCCTGTTTTACGTTCAATGTGTAA	HTTNRYHYICLWFSCFTFNV*	-1.159	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26680	HIPNSQPAFRTLRTYTLYTW			20	SLAY-screened peptide P5030			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATATCCCGAATAGCCAGCCCGCCTTTAGGACGCTCCGTACGTACACGCTCTATACCTGGTAA	HIPNSQPAFRTLRTYTLYTW*	-1.159	0.000291	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26681	SPVMSKYAIPSYRPMFPPIK			20	SLAY-screened peptide P5031			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCCTGTTATGTCTAAGTACGCTATCCCCTCGTACCGTCCCATGTTCCCTCCGATCAAGTAA	SPVMSKYAIPSYRPMFPPIK*	-1.159	0.000133	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26682	SAALPLIDYVYRAYIENASR			20	SLAY-screened peptide P5032			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCTGCCCTGCCTCTTATTGACTATGTGTATCGCGCGTACATCGAGAACGCCAGTCGCTAA	SAALPLIDYVYRAYIENASR*	-1.159	0.02708	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26683	VYSTGFGSSESCSRPHSQYY			20	SLAY-screened peptide P5033			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTACAGTACGGGGTTCGGCTCTAGTGAGAGTTGCAGCCGTCCTCATTCCCAGTATTATTAA	VYSTGFGSSESCSRPHSQYY*	-1.158	0.00011	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26684	SIHNHPCSTRANP			13	SLAY-screened peptide P5034			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTATCCATAACCATCCGTGTTCTACCCGCGCTAATCCCTAGGATTCTTTTCATGAGCGGTAA	SIHNHPCSTRANP*DSFHER*	-1.158	0.043984	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26685	CHLAPST			7	SLAY-screened peptide P5035			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCACTTGGCCCCTAGCACTTAGGGTTAGCCTAACCGTACCTTTATTACCTCTAGCTCCTAA	CHLAPST*G*PNRTFITSSS*	-1.158	0.034505	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26686	LFSCLTYYM			9	SLAY-screened peptide P5036			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGTTTAGTTGTCTCACGTACTACATGTAGTCGACCTATTTCCAGTACTCTGTGCACCGTTAA	LFSCLTYYM*STYFQYSVHR*	-1.157	0.029924	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26687	LPVRSHNYGNYMSN			14	SLAY-screened peptide P5037			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCTGTTAGGTCGCATAATTACGGCAACTACATGTCTAACTAGAGTTTGGTTTACCATAAC	LPVRSHNYGNYMSN*SLVYHN	-1.157	0.000449	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26688	TLYPITHCYHHCHPLCYTDH			20	SLAY-screened peptide P5038			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTTGTACCCTATTACTCATTGTTACCACCACTGTCACCCGTTGTGCTACACGGACCACTAA	TLYPITHCYHHCHPLCYTDH*	-1.157	0.016332	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26689	TLPPSPSPTNPGADLLPASS			20	SLAY-screened peptide P5039			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTTCCGCCTTCCCCGTCGCCGACCAACCCGGGCGCGGACCTTCTTCCCGCGTCGAGTTAA	TLPPSPSPTNPGADLLPASS*	-1.157	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26690	LCGLRPTPHVLIHTPTYLTL			20	SLAY-screened peptide P5040			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTGTGGCCTCCGGCCGACGCCCCACGTGCTGATCCATACTCCCACCTATTTGACTTTGTAA	LCGLRPTPHVLIHTPTYLTL*	-1.157	0.006484	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26691	FPPLRLDTNYPWCHPSTLYW			20	SLAY-screened peptide P5041			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCCTCCCCTGAGGTTGGACACGAATTATCCCTGGTGTCATCCCTCTACCCTCTATTGGTAA	FPPLRLDTNYPWCHPSTLYW*	-1.157	0.001469	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26692	SFCNALYPRPFARHDCMCHP			20	SLAY-screened peptide P5042			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTTCTGCAATGCTCTCTACCCTCGCCCTTTTGCGCGCCACGACTGTATGTGTCACCCCTAA	SFCNALYPRPFARHDCMCHP*	-1.156	0.002208	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26693	QGHSHM			6	SLAY-screened peptide P5043			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGGTCACAGCCACATGTAGGGGACGCGCGCCATCCACAACCTCCGCCTGGGTTAGGCTTAA	QGHSHM*GTRAIHNLRLG*A*	-1.156	1.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26694	YLPALCSPFHNRSQHFHCCL			20	SLAY-screened peptide P5044			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATCTCCCTGCGTTGTGCAGCCCCTTTCACAATCGCTCGCAGCATTTTCATTGTTGTCTTTAA	YLPALCSPFHNRSQHFHCCL*	-1.156	0.008382	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26695	PLLLWYFCFLLSAETTYNKD			20	SLAY-screened peptide P5045			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTGCTCTTGTGGTACTTTTGCTTCCTGCTTAGTGCGGAGACCACCTACAATAAGGACTAA	PLLLWYFCFLLSAETTYNKD*	-1.155	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26696	LNAPTDDITSPRNHYGLNDY			20	SLAY-screened peptide P5046			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAATGCGCCGACTGACGACATCACCTCTCCCCGGAACCACTATGGTCTTAACGATTATTAA	LNAPTDDITSPRNHYGLNDY*	-1.155	0.037769	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26697	PLMYLVSYVLNLLLSTSSGR			20	SLAY-screened peptide P5047			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTGATGTATCTGGTCAGCTATGTGCTCAATCTTCTTTTGTCCACCTCGAGTGGGCGTTAA	PLMYLVSYVLNLLLSTSSGR*	-1.155	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26698	TTRTRCSTPVDPTLHLNS			18	SLAY-screened peptide P5048			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGACGCGTACTCGCTGCAGCACTCCTGTGGACCCCACCTTGCATCTTAATAGTTAGTTTTAA	TTRTRCSTPVDPTLHLNS*F*	-1.155	0.006545	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26699	VADSVHPSFAPNHATTFSAR			20	SLAY-screened peptide P5049			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGGCTGACTCCGTGCATCCTAGTTTTGCTCCTAACCACGCCACGACTTTCTCGGCCCGCTAA	VADSVHPSFAPNHATTFSAR*	-1.154	0.000109	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26700	PWRCYPHQFPTARRPPLRIV			20	SLAY-screened peptide P5050			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGGAGGTGCTATCCTCACCAGTTTCCCACCGCCCGGAGGCCGCCCCTCCGCATCGTCTAA	PWRCYPHQFPTARRPPLRIV*	-1.154	0.000208	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26701	PPHCHPTRTCKTCVPPRDNR			20	SLAY-screened peptide P5051			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCCCACTGTCACCCTACTCGTACGTGCAAGACGTGCGTCCCGCCCCGCGACAACCGTTAA	PPHCHPTRTCKTCVPPRDNR*	-1.154	4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26702	SRRPYANTLNSTFLTLLQEH			20	SLAY-screened peptide P5052			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCGTCGCCCGTACGCCAACACGTTGAACTCCACTTTTCTTACTCTCCTCCAGGAGCACTAA	SRRPYANTLNSTFLTLLQEH*	-1.154	0.000236	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26703	PAGHSPPPCYLFANADMRVI			20	SLAY-screened peptide P5053			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCCGGTCATTCGCCGCCGCCTTGCTATTTGTTTGCTAACGCTGATATGAGGGTGATTTAA	PAGHSPPPCYLFANADMRVI*	-1.154	0.01791	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26704	LHESCTLGRLNHEGYNIIVV			20	SLAY-screened peptide P5054			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCACGAGTCCTGCACTCTCGGGCGCTTGAACCATGAGGGCTACAATATTATTGTCGTTTAA	LHESCTLGRLNHEGYNIIVV*	-1.154	0.018752	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26705	ASLLIPSGSGNRSHSSFYDM			20	SLAY-screened peptide P5055			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAGCCTTCTTATTCCTTCGGGGTCCGGCAATCGGAGTCACTCCAGTTTCTACGATATGTAA	ASLLIPSGSGNRSHSSFYDM*	-1.154	0.003355	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26706	NRPFSLCPLFYMSPVSPINW			20	SLAY-screened peptide P5056			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCGCCCGTTTTCCCTGTGTCCGCTTTTCTACATGAGTCCCGTGAGCCCCATTAATTGGTAA	NRPFSLCPLFYMSPVSPINW*	-1.153	0.000284	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26707	TTDVEVCTSPSFASVSSHCH			20	SLAY-screened peptide P5057			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACGGATGTCGAGGTTTGCACTTCCCCGTCTTTTGCCTCGGTCTCGAGCCACTGCCATTAA	TTDVEVCTSPSFASVSSHCH*	-1.153	0.004701	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26708	LSVTGLNGIMTGKINPPLCL			20	SLAY-screened peptide P5058			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCTGTTACCGGTCTCAACGGTATCATGACTGGCAAGATCAACCCTCCCCTCTGCCTCTAA	LSVTGLNGIMTGKINPPLCL*	-1.153	0.001483	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26709	CRIYPIGA			8	SLAY-screened peptide P5059			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGTATTTATCCCATTGGTGCTTAGCGCGTCGCCTCTCAGACCATCGGTAAGATGTACTAA	CRIYPIGA*RVASQTIGKMY*	-1.153	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26710	IPISCWAPCYPASHDWECSL			20	SLAY-screened peptide P5060			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCCCATCTCCTGTTGGGCTCCGTGCTATCCTGCGTCTCACGACTGGGAGTGCTCTCTCTAA	IPISCWAPCYPASHDWECSL*	-1.153	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26711	FYCTALPYATSRTVGNDNAI			20	SLAY-screened peptide P5061			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTATTGTACGGCTCTGCCTTATGCTACTTCTCGTACGGTGGGTAATGACAATGCCATTTAA	FYCTALPYATSRTVGNDNAI*	-1.153	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26712	DP			2	SLAY-screened peptide P5062			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCCCTAGCATCCTGGCACTATTCCTGAGCAGCTCCCGCTGCCCCACCGCTGGGGCTTGTAA	DP*HPGTIPEQLPLPHRWGL*	-1.152	3.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26713	CFGIPCCASPRGSHPLAIYV			20	SLAY-screened peptide P5063			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTTCGGGATCCCGTGCTGCGCTTCGCCCCGCGGTTCTCACCCGCTGGCCATTTACGTTTAA	CFGIPCCASPRGSHPLAIYV*	-1.152	0.0002	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26714	TALLLTSLATILRELRCHGH			20	SLAY-screened peptide P5064			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGCGCTTTTGTTGACGAGTCTGGCGACGATTCTCCGTGAGCTCAGGTGTCACGGGCATTAA	TALLLTSLATILRELRCHGH*	-1.151	0.022762	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26715	PYNSMRHCSFHADNHSQRWL			20	SLAY-screened peptide P5065			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTATAACTCGATGCGCCACTGCTCCTTTCATGCTGACAATCATAGCCAGCGTTGGTTGTAA	PYNSMRHCSFHADNHSQRWL*	-1.151	0.001107	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26716	CTGPHSHSLVYIGACTPICM			20	SLAY-screened peptide P5066			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTACGGGCCCTCATTCCCATAGCCTCGTGTACATTGGTGCCTGTACGCCGATTTGTATGTAA	CTGPHSHSLVYIGACTPICM*	-1.151	0.000636	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26717	SNREGVGWSCLPVSPL			16	SLAY-screened peptide P5067			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTAATCGCGAAGGGGTAGGGTGGTCATGCCTGCCCGTTTCACCTTTGTAACTATAACTAACT	SNREGVGWSCLPVSPL*L*LT	-1.151	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26718	PRRVFPHLQDNQDYAKNAIL			20	SLAY-screened peptide P5068			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAGGAGGGTCTTCCCCCATTTGCAGGATAATCAGGACTATGCTAAGAATGCTATTTTGTAA	PRRVFPHLQDNQDYAKNAIL*	-1.151	0.001416	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26719	THHYSYQGLSANI			13	SLAY-screened peptide P5069			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCACCACTACAGTTATCAGGGGTTGTCCGCTAACATCTAGCTCATTGGTTCCTAGTACTAA	THHYSYQGLSANI*LIGS*Y*	-1.151	0.000677	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26720	SPDLDPFQTPTILIIFPGTR			20	SLAY-screened peptide P5070			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCCCGATCTTGACCCTTTTCAGACTCCGACGATCCTGATTATTTTTCCCGGGACTAGGTAA	SPDLDPFQTPTILIIFPGTR*	-1.151	0.008652	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26721	DNSFQHCELVPSCYKCPPQF			20	SLAY-screened peptide P5071			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATAACTCCTTTCAGCATTGCGAGCTTGTGCCTAGCTGCTATAAGTGTCCCCCTCAGTTCTAA	DNSFQHCELVPSCYKCPPQF*	-1.151	0.047156	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26722	SAPCWWRYMAAPHDHHTYNP			20	SLAY-screened peptide P5072			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGCTCCGTGTTGGTGGCGTTACATGGCGGCCCCTCACGACCACCATACTTACAATCCTTAA	SAPCWWRYMAAPHDHHTYNP*	-1.15	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26723	PVMA			4	SLAY-screened peptide P5073			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGTGATGGCTTAGCTGAAGGACCACCCGAACGTGACCCACAACATCTTTAATGTGCCGTAA	PVMA*LKDHPNVTHNIFNVP*	-1.15	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26724	YWTPLPVLFRCLLSKLKNCI			20	SLAY-screened peptide P5074			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTGGACCCCCTTGCCCGTGCTCTTCCGTTGTTTGCTGTCGAAGCTGAAGAACTGTATTTAA	YWTPLPVLFRCLLSKLKNCI*	-1.15	0.000275	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26725	HAPNNTIEAYCLLPYPDTLT			20	SLAY-screened peptide P5075			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCTCCTAACAATACCATTGAGGCGTATTGCCTCCTTCCCTATCCTGATACGCTCACGTAA	HAPNNTIEAYCLLPYPDTLT*	-1.15	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26726	LFLNLLCPKKILPLCIALTTN			21	SLAY-screened peptide P5076			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTTCCTCAACCTTCTGTGTCCTAAGAAGATCCTACCTCTATGCATCGCACTTACAACTAAC	LFLNLLCPKKILPLCIALTTN	-1.15	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26727	AACPGLVRVHPDLVLTYHRR			20	SLAY-screened peptide P5077			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCCTGTCCGGGCCTTGTTCGCGTCCACCCCGATCTGGTCCTGACTTACCATCGCAGGTAA	AACPGLVRVHPDLVLTYHRR*	-1.149	0.004447	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26728	NCWSNISTALTTLWKRFVAI			20	SLAY-screened peptide P5078			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTGCTGGAGTAACATTAGCACCGCCTTGACTACTCTTTGGAAGCGGTTTGTTGCGATCTAA	NCWSNISTALTTLWKRFVAI*	-1.149	4.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26729	SRYMFCHPPTWTSVVRNYSI			20	SLAY-screened peptide P5079			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCGTTATATGTTTTGCCACCCTCCCACGTGGACCTCTGTGGTTCGTAACTATTCCATCTAA	SRYMFCHPPTWTSVVRNYSI*	-1.148	0.033533	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26730	SLAYIINRALRHIVTSSCTL			20	SLAY-screened peptide P5080			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTTGGCGTACATCATTAACCGCGCTCTTCGCCATATCGTTACTTCTAGTTGTACGCTCTAA	SLAYIINRALRHIVTSSCTL*	-1.148	0.00237	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26731	TIPGRPVCLVVKSNSYF			17	SLAY-screened peptide P5081			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTATCCCCGGGCGCCCTGTCTGTCTTGTGGTGAAGTCTAATTCCTATTTCTAGGCTACTTAA	TIPGRPVCLVVKSNSYF*AT*	-1.148	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26732	PASISI			6	SLAY-screened peptide P5082			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGCGTCTATCTCCATCTAGCACAATAATGACAATGCGACCCCCTTGGGCACGTTTGTCTAA	PASISI*HNNDNATPLGTFV*	-1.148	0.00613	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26733	RRHRGLCMLCMPIIASMI			18	SLAY-screened peptide P5083			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGGCATCGGGGTCTGTGTATGCTCTGCATGCCGATCATCGCGTCCATGATTTAGTGTTAA	RRHRGLCMLCMPIIASMI*C*	-1.148	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26734	RHLTSTG			7	SLAY-screened peptide P5084			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCACCTTACGTCGACCGGCTAGCTTCGCAACATGTTTACCAACCACATGTACAGGACCTAA	RHLTSTG*LRNMFTNHMYRT*	-1.147	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26735	LRPIPILSVNLLHDAVPTHS			20	SLAY-screened peptide P5085			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCGGCCGATTCCGATTCTTTCGGTCAACCTCCTGCATGATGCTGTCCCCACCCATTCTTAA	LRPIPILSVNLLHDAVPTHS*	-1.147	0.01901	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26736	WYPLPYNYYHCNHTHLHHFN			20	SLAY-screened peptide P5086			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTATCCCCTCCCTTACAACTATTACCACTGTAACCACACGCACCTGCATCATTTCAATTAA	WYPLPYNYYHCNHTHLHHFN*	-1.147	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26737	CWPRPSFGFYRYPDWLLCRA			20	SLAY-screened peptide P5087			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTGGCCTCGTCCTTCGTTCGGGTTCTATCGTTACCCCGATTGGCTGTTGTGCCGTGCCTAA	CWPRPSFGFYRYPDWLLCRA*	-1.147	0.001059	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26738	NPTITNDQPNSHGVRGAFSS			20	SLAY-screened peptide P5088			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCCTACGATTACGAACGATCAGCCGAACAGCCATGGTGTCCGCGGGGCCTTCAGCAGTTAA	NPTITNDQPNSHGVRGAFSS*	-1.147	0.001525	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26739	LDLTDYTVSTCMLNGSR			17	SLAY-screened peptide P5089			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGACCTGACGGATTACACCGTGTCCACTTGTATGCTTAATGGGAGCCGTTAGACTGATTAA	LDLTDYTVSTCMLNGSR*TD*	-1.147	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26740	GNHGSSGYTKHYTVYSVFVI			20	SLAY-screened peptide P5090			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTAACCACGGCTCTTCGGGTTATACCAAGCACTACACCGTTTACAGCGTGTTTGTGATTTAA	GNHGSSGYTKHYTVYSVFVI*	-1.146	0.000402	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26741	PGFLLSELKRVVRGIIGIY			19	SLAY-screened peptide P5091			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGCTTCCTTCTTAGCGAGCTCAAGAGGGTTGTTCGTGGCATTATTGGTATCTACTAGTAA	PGFLLSELKRVVRGIIGIY**	-1.146	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26742	KLY			3	SLAY-screened peptide P5092			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCTGTATTAGTGTCTTTTCTCCAACTCCCCTAAGTTCTTGTGGCGTGCTTGTTTTTAGTAA	KLY*CLFSNSPKFLWRACF**	-1.146	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26743	HEQFDVSRPDILLCTRHAAN			20	SLAY-screened peptide P5093			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGAGCAGTTTGATGTGTCGCGTCCCGATATTTTGCTTTGTACCAGGCACGCTGCTAACTAA	HEQFDVSRPDILLCTRHAAN*	-1.146	0.028802	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26744	VPTYRDRDHSYFSYCLI			17	SLAY-screened peptide P5094			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCCGACCTACCGTGACCGTGACCATTCGTACTTTTCGTACTGCCTGATCTAGATGAGTTAA	VPTYRDRDHSYFSYCLI*MS*	-1.145	0.026108	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26745	VDANRHLISTVARSTVSSRVN			21	SLAY-screened peptide P5095			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGACGCCAACAGGCATCTCATATCGACGGTTGCGCGATCGACTGTTTCGTCAAGAGTTAAC	VDANRHLISTVARSTVSSRVN	-1.145	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26746	PLKSKMLCVVLL			12	SLAY-screened peptide P5096			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTGAAGAGTAAGATGCTTTGCGTGGTGCTCTTGTAGATGTTCCGGGCCGAGACGCATTAA	PLKSKMLCVVLL*MFRAETH*	-1.145	0.029682	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26747	PLLHCYPLYGAHTCHCYHTI			20	SLAY-screened peptide P5097			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCTCTTGCACTGTTACCCTTTGTACGGGGCCCACACCTGCCATTGCTACCATACCATTTAA	PLLHCYPLYGAHTCHCYHTI*	-1.145	0.000321	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26748	LALILSLF			8	SLAY-screened peptide P5098			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGCGCTTATTTTGAGTCTCTTTTAGACCCACGCTGATTTTGATAGTGCGTACATGGTGTAA	LALILSLF*THADFDSAYMV*	-1.144	0.023045	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26749	WVSSLMFNIYDGSFCHPSSC			20	SLAY-screened peptide P5099			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGGTTTCGAGTCTCATGTTTAATATCTACGATGGTTCCTTCTGCCATCCCAGCAGCTGTTAA	WVSSLMFNIYDGSFCHPSSC*	-1.144	4.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26750	PCDYHFILIPQNPGDAPYLIY			21	SLAY-screened peptide P5100			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGTGACTATCATTTTATTCTTATCCCCCAGAATCCTGGCGACGCCCCTTACCTTATTTAC	PCDYHFILIPQNPGDAPYLIY	-1.144	0.016741	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26751	HPSPNHVHKFEGPATTLGVN			20	SLAY-screened peptide P5101			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCTTCTCCCAATCACGTCCATAAGTTTGAGGGTCCTGCTACGACGCTTGGCGTTAATTAA	HPSPNHVHKFEGPATTLGVN*	-1.144	0.007873	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26752	ITTQLILTFELNIVTIIIHS			20	SLAY-screened peptide P5102			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCACTACCCAGCTCATCTTGACCTTCGAGCTTAATATCGTTACGATCATCATTCATTCTTAA	ITTQLILTFELNIVTIIIHS*	-1.143	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26753	QVTNDSGSYYNSNFGVSLDQ			20	SLAY-screened peptide P5103			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGTCACGAATGACTCCGGTTCGTACTACAATTCTAACTTTGGTGTTTCCCTTGACCAGTAA	QVTNDSGSYYNSNFGVSLDQ*	-1.143	0.002456	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26754	PSLTARTLYFMEPDLIDSNA			20	SLAY-screened peptide P5104			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAGCCTGACCGCTCGTACTCTTTATTTTATGGAGCCTGATTTGATCGACTCCAACGCGTAA	PSLTARTLYFMEPDLIDSNA*	-1.143	0.001668	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26755	RSRDEYVSGFSSHLIFYKNQ			20	SLAY-screened peptide P5105			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCGCGGGACGAGTACGTTAGTGGCTTTAGTTCGCATCTGATCTTCTATAAGAATCAGTAA	RSRDEYVSGFSSHLIFYKNQ*	-1.143	0.033865	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26756	NRKYKVSNRNHCNCHTTNHK			20	SLAY-screened peptide P5106			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCGTAAGTACAAGGTGTCCAATCGCAATCATTGCAATTGCCACACCACTAACCATAAGTAA	NRKYKVSNRNHCNCHTTNHK*	-1.143	0.006409	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26757	GAINRTCASCHWLPCLIGYI			20	SLAY-screened peptide P5107			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGCGATCAATCGGACCTGTGCCAGCTGTCATTGGTTGCCTTGTCTTATCGGGTACATCTAA	GAINRTCASCHWLPCLIGYI*	-1.143	0.001757	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26758	LTVLSSRTCGTILSHYCT			18	SLAY-screened peptide P5108			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGACGGTGCTGTCTTCGCGCACGTGCGGCACTATTTTGTCGCATTATTGCACCTAGCTTTAA	LTVLSSRTCGTILSHYCT*L*	-1.143	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26759	PYSGHYFSIHAGASTHE			17	SLAY-screened peptide P5109			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTACTCGGGTCATTACTTCTCGATTCATGCCGGTGCGTCTACGCACGAGTAGTGTATTTAA	PYSGHYFSIHAGASTHE*CI*	-1.143	0.003386	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26760	HWLSTNRSPYAHTCIPVYTW			20	SLAY-screened peptide P5110			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGGTTGTCGACTAATCGGTCCCCGTATGCCCATACTTGCATTCCCGTTTACACCTGGTAA	HWLSTNRSPYAHTCIPVYTW*	-1.142	0.020743	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26761	ETSGAVRYCTPFISPYWLCC			20	SLAY-screened peptide P5111			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGACCAGTGGTGCCGTCCGCTACTGTACTCCGTTTATCTCGCCCTATTGGTTGTGTTGCTAA	ETSGAVRYCTPFISPYWLCC*	-1.142	3.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26762	CHDS			4	SLAY-screened peptide P5112			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCACGACTCTTAGTTGATGGGGAACTATACGGTTACGTGTCCGTTTCTTCTTGGGTTTTAA	CHDS*LMGNYTVTCPFLLGF*	-1.142	7.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26763	VCSCPILPINTRYGRRLLLS			20	SLAY-screened peptide P5113			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTGCTCCTGCCCCATCCTCCCTATTAACACGCGCTATGGCCGCCGCTTGCTTCTGTCGTAA	VCSCPILPINTRYGRRLLLS*	-1.142	0.004696	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26764	TPRFYPLIFSYI			12	SLAY-screened peptide P5114			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCTCGCTTTTATCCCCTCATTTTTTCTTACATTTAGGTCTTTAATACCTGTACGTTCTAA	TPRFYPLIFSYI*VFNTCTF*	-1.142	0.042007	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26765	CAPYTKPHKTSMRPLDTFKT			20	SLAY-screened peptide P5115			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGCGCCTTACACGAAGCCCCACAAGACTAGTATGCGGCCCCTTGACACCTTTAAGACTTAA	CAPYTKPHKTSMRPLDTFKT*	-1.142	0.044403	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26766	SERTCPNVTHTVLPVPTSTA			20	SLAY-screened peptide P5116			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGAGCGCACTTGCCCTAATGTCACTCATACTGTTCTGCCCGTCCCCACCTCCACTGCTTAA	SERTCPNVTHTVLPVPTSTA*	-1.142	0.023248	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26767	LPRGPPGRCNPWDGDWCHGL			20	SLAY-screened peptide P5117			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCCCCGCGGGCCGCCGGGTCGTTGCAACCCCTGGGATGGCGATTGGTGCCACGGTCTTTAA	LPRGPPGRCNPWDGDWCHGL*	-1.141	0.003336	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26768	CTSLYTYL			8	SLAY-screened peptide P5118			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTACCTCTCTTTACACGTATCTCTAGCCGTTCATCCGTCTTTTGAATTGCTGCAGTGACTAA	CTSLYTYL*PFIRLLNCCSD*	-1.141	0.023156	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26769	SS			2	SLAY-screened peptide P5119			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTCCTAGCACCTCCCCATGCCTCGTACCGCGATGCACGCCCACCCGGACACGCTCACCTAA	SS*HLPMPRTAMHAHPDTLT*	-1.141	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26770	PFNHADDAEPHFLHTIWHYM			20	SLAY-screened peptide P5120			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTCAACCATGCTGACGACGCGGAGCCTCACTTTCTCCACACCATTTGGCATTATATGTAA	PFNHADDAEPHFLHTIWHYM*	-1.14	0.014675	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26771	HSNDLFSKDTSCYHPCDRPY			20	SLAY-screened peptide P5121			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTCGAACGATCTTTTTAGTAAGGATACCTCCTGCTATCACCCTTGTGATCGGCCTTACTAA	HSNDLFSKDTSCYHPCDRPY*	-1.14	0.035197	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26772	SGMCDNYDLPIRPPWFRRRRN			21	SLAY-screened peptide P5122			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGGCATGTGTGATAATTATGACCTCCCCATCAGGCCCCCGTGGTTCAGGCGGCGACGTAAC	SGMCDNYDLPIRPPWFRRRRN	-1.14	0.024085	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26773	LNQPTTTPSSG			11	SLAY-screened peptide P5123			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAACCAACCAACTACGACCCCTTCTAGCGGGTGATCCTTCACAACAACAATCACGCGTAAC	LNQPTTTPSSG*SFTTTITRN	-1.139	0.000627	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26774	FACPDFIRKCRVCMDNFRTA			20	SLAY-screened peptide P5124			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGCCTGTCCGGACTTTATCCGTAAGTGCCGTGTTTGTATGGACAACTTTCGTACCGCTTAA	FACPDFIRKCRVCMDNFRTA*	-1.139	0.048457	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26775	NPGCYFALLHSDNILATHIS			20	SLAY-screened peptide P5125			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCCCGGTTGCTATTTTGCGTTGCTGCACAGCGATAATATTTTGGCGACCCATATTTCCTAA	NPGCYFALLHSDNILATHIS*	-1.139	0.007475	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26776	CVYGDSQHLSVSDPDPFTLI			20	SLAY-screened peptide P5126			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGTCTACGGCGATTCCCAGCACCTGTCGGTCTCGGACCCCGACCCCTTCACTTTGATTTAA	CVYGDSQHLSVSDPDPFTLI*	-1.139	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26777	VFLSLLMIAKYILLNAINHK			20	SLAY-screened peptide P5127			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTTCCTGTCCCTGCTTATGATCGCGAAGTACATTCTGTTGAATGCGATCAATCACAAGTAA	VFLSLLMIAKYILLNAINHK*	-1.139	0.004724	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26778	TSVHH			5	SLAY-screened peptide P5128			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTCGGTTCATCACTAGGAGAAGGATCGGGTCAATTAGACTATCCTGTTGTACTTTTTGTAA	TSVHH*EKDRVN*TILLYFL*	-1.139	0.002854	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26779	CNFGLHGVACFYFTDLLFTP			20	SLAY-screened peptide P5129			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCAACTTCGGTCTGCACGGTGTGGCGTGTTTTTATTTCACGGATCTGCTCTTCACTCCTTAA	CNFGLHGVACFYFTDLLFTP*	-1.139	0.006724	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26780	VAPTFSSVRLPLCFILCLTL			20	SLAY-screened peptide P5130			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGCTCCTACCTTTTCTTCCGTGCGTCTCCCTCTCTGTTTCATTCTCTGCTTGACTCTGTAA	VAPTFSSVRLPLCFILCLTL*	-1.139	0.010685	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26781	HLLETTALAYL			11	SLAY-screened peptide P5131			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCTTCTCGAGACGACCGCTCTTGCCTATCTGTAGAGTACGCTCACCGCGTCTATGCCGTAA	HLLETTALAYL*STLTASMP*	-1.138	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26782	PQPASGCPTNSGAARPNSC			19	SLAY-screened peptide P5132			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCAGCCGGCTAGCGGCTGCCCTACTAATTCGGGGGCCGCCCGCCCTAACTCTTGCTAACTG	PQPASGCPTNSGAARPNSC*L	-1.138	0.011149	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26783	PPRHCARRDYDFDFPYLWTY			20	SLAY-screened peptide P5133			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCCCGTCACTGTGCCCGCCGCGACTACGACTTCGACTTCCCTTATTTGTGGACTTACTAA	PPRHCARRDYDFDFPYLWTY*	-1.138	0.001091	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26784	PSDDQPPNSLTLTCAIPRR			19	SLAY-screened peptide P5134			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGTGATGACCAGCCTCCGAATTCCCTTACGTTGACGTGCGCGATCCCCCGTCGGTAGTAA	PSDDQPPNSLTLTCAIPRR**	-1.138	9.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26785	HCRTWTNAVLASQHNVTVHS			20	SLAY-screened peptide P5135			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGCCGTACGTGGACGAACGCGGTTTTGGCCTCCCAGCATAATGTCACCGTGCACTCTTAA	HCRTWTNAVLASQHNVTVHS*	-1.138	0.001689	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26786	PCPRSYNLATPLSYLAQRLQ			20	SLAY-screened peptide P5136			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGCCCCCGCTCGTACAACCTGGCGACCCCGCTCTCCTACCTTGCGCAGCGTCTCCAGTAA	PCPRSYNLATPLSYLAQRLQ*	-1.138	0.010744	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26787	TFKAEMILRHCYDYDNRHSSN			21	SLAY-screened peptide P5137			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTTCAAGGCCGAGATGATCCTTAGGCACTGCTATGATTATGACAACCGCCACTCTTCTAAC	TFKAEMILRHCYDYDNRHSSN	-1.138	0.035271	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26788	SILAKVYSLLGQHKTCNRDG			20	SLAY-screened peptide P5138			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTATTCTGGCTAAGGTTTACAGTCTGCTCGGGCAGCACAAGACTTGCAACAGGGATGGCTAA	SILAKVYSLLGQHKTCNRDG*	-1.137	8.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26789	RESPRPGCWCYIPAPELCLL			20	SLAY-screened peptide P5139			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGAGTCGCCGCGGCCTGGGTGCTGGTGTTATATTCCGGCCCCTGAGCTTTGTTTGCTTTAA	RESPRPGCWCYIPAPELCLL*	-1.137	0.006368	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26790	EKGKERGYPLKPYETRLLCP			20	SLAY-screened peptide P5140			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGAAGGGCAAGGAGCGCGGTTATCCGCTCAAGCCCTACGAGACGAGGTTGCTTTGCCCCTAA	EKGKERGYPLKPYETRLLCP*	-1.137	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26791	YCPPSSTNLNDMHLASTAMW			20	SLAY-screened peptide P5141			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTGCCCTCCCTCCTCGACCAACCTTAACGACATGCATCTTGCTAGTACTGCTATGTGGTAA	YCPPSSTNLNDMHLASTAMW*	-1.137	0.026136	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26792	SPTCTSLPDDLGTMLIINRA			20	SLAY-screened peptide P5142			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCCTACCTGCACCTCGCTGCCGGATGATCTGGGTACCATGTTGATCATCAATCGCGCTTAA	SPTCTSLPDDLGTMLIINRA*	-1.137	0.002355	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26793	HCPRWHPPHYYTREDLTLLR			20	SLAY-screened peptide P5143			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTGCCCTCGGTGGCACCCCCCTCACTACTACACCAGGGAGGATCTGACCCTTCTCAGGTAA	HCPRWHPPHYYTREDLTLLR*	-1.136	5.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26794	GAFSTPLSVDSATIPCDRY			19	SLAY-screened peptide P5144			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGCCTTCTCTACCCCTTTGTCCGTTGACAGTGCCACCATCCCTTGCGATCGTTATTAGTAA	GAFSTPLSVDSATIPCDRY**	-1.136	0.001829	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26795	SAHSCYDLVGIHYAARPIVG			20	SLAY-screened peptide P5145			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGCCCATAGCTGTTATGATTTGGTTGGCATTCATTACGCTGCCCGTCCTATCGTTGGGTAA	SAHSCYDLVGIHYAARPIVG*	-1.136	0.011653	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26796	PISGNWTIYPVFVSTPIIYS			20	SLAY-screened peptide P5146			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATCAGCGGCAATTGGACTATTTATCCTGTTTTTGTTAGTACTCCTATTATTTATAGTTAA	PISGNWTIYPVFVSTPIIYS*	-1.136	0.000137	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26797	LHYKR			5	SLAY-screened peptide P5147			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCATTATAAGCGCTAGACGGTGGCTAGTAACGTGGATAGGTTTACCAAGCTCGCCAGGTAA	LHYKR*TVASNVDRFTKLAR*	-1.135	0.008096	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26798	YIFPLSTTTFCGHFMRYCNS			20	SLAY-screened peptide P5148			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACATTTTCCCGCTCTCCACGACTACCTTTTGTGGCCACTTCATGAGGTACTGTAACTCGTAA	YIFPLSTTTFCGHFMRYCNS*	-1.135	0.000457	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26799	DVRPDPWRAPQTLCIPLIYT			20	SLAY-screened peptide P5149			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGTCCGTCCTGATCCCTGGCGGGCGCCTCAGACCTTGTGCATTCCTCTTATTTATACCTAA	DVRPDPWRAPQTLCIPLIYT*	-1.135	0.016572	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26800	AKSNDCRHKPFWTTYDYMQV			20	SLAY-screened peptide P5150			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTAAGTCGAATGATTGTCGTCACAAGCCCTTCTGGACTACGTACGACTACATGCAGGTCTAA	AKSNDCRHKPFWTTYDYMQV*	-1.135	0.039563	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26801	LRHSAELPYSIYSKYQYFTI			20	SLAY-screened peptide P5151			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCGTCACTCGGCTGAGTTGCCCTATTCTATCTACAGTAAGTATCAGTATTTTACCATTTAA	LRHSAELPYSIYSKYQYFTI*	-1.135	0.002672	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26802	NIISDLMRKANILISKIMCW			20	SLAY-screened peptide P5152			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATATCATCTCTGACCTTATGCGGAAGGCCAACATCCTTATTTCTAAGATCATGTGTTGGTAA	NIISDLMRKANILISKIMCW*	-1.135	0.000467	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26803	LMSPWILLLDAHWFEHFFAY			20	SLAY-screened peptide P5153			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATGAGCCCGTGGATTCTCCTTCTCGATGCTCACTGGTTCGAGCACTTTTTTGCCTATTAA	LMSPWILLLDAHWFEHFFAY*	-1.134	0.001901	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26804	AIGTYGLHEYSHSFPLHMCF			20	SLAY-screened peptide P5154			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTATTGGGACCTACGGTTTGCACGAGTATAGCCATTCCTTCCCTCTTCATATGTGCTTTTAA	AIGTYGLHEYSHSFPLHMCF*	-1.134	0.001583	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26805	RLISRLFLALILRLIGSVRVN			21	SLAY-screened peptide P5155			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGCCGGCTCATTTCCCGCCTCTTCCTCGCGCTCATACTGAGACTTATTGGGTCAGTGCGAGTTAAC	RLISRLFLALILRLIGSVRVN	-1.134	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26806	TGTIVNYFFIFC			12	SLAY-screened peptide P5156			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGGTACTATCGTTAATTACTTTTTCATTTTCTGCTAGAACCGGAACGCTCCCACTTACTAA	TGTIVNYFFIFC*NRNAPTY*	-1.134	0.031916	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26807	SGAHWP			6	SLAY-screened peptide P5157			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGGCGCCCACTGGCCGTAGCGCTCCCCCAAGTTTATCTACCCGTATAAGTTTGTCCACTAA	SGAHWP*RSPKFIYPYKFVH*	-1.134	8.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26808	WPGYTDCWCQAQMHRFSQCY			20	SLAY-screened peptide P5158			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCCCGGTTACACTGACTGCTGGTGCCAGGCGCAGATGCACAGGTTTAGCCAGTGCTATTAA	WPGYTDCWCQAQMHRFSQCY*	-1.134	0.001798	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26809	PFISFVYPSRHLCHASNSSD			20	SLAY-screened peptide P5159			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTTCATCTCTTTCGTTTACCCTTCCAGGCACCTTTGTCACGCCAGCAATTCGAGTGATTAA	PFISFVYPSRHLCHASNSSD*	-1.134	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26810	APSGCARNLCKVSMTESSTL			20	SLAY-screened peptide P5160			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCCGAGTGGCTGCGCTCGTAACCTGTGTAAGGTCTCCATGACGGAGAGCTCGACTCTCTAA	APSGCARNLCKVSMTESSTL*	-1.134	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26811	CWNRSYGSARGIRIFATRIN			20	SLAY-screened peptide P5161			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTGGAACCGCTCTTATGGTAGTGCCAGAGGAATACGCATATTTGCAACCCGCATTAACTGA	CWNRSYGSARGIRIFATRIN*	-1.134	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26812	PDLDVDPSFHYTDTIYSWPC			20	SLAY-screened peptide P5162			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGACCTTGACGTCGACCCTAGCTTTCATTACACGGATACCATTTATAGCTGGCCCTGCTAA	PDLDVDPSFHYTDTIYSWPC*	-1.133	0.015519	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26813	RSLRYWDHVHLTLDPRYMCI			20	SLAY-screened peptide P5163			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCGCTGCGCTACTGGGACCATGTTCACTTGACCCTCGACCCTCGCTATATGTGCATCTAA	RSLRYWDHVHLTLDPRYMCI*	-1.133	0.011946	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26814	ATGRLGNHRTQRWDLARFTV			20	SLAY-screened peptide P5164			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACCGGCCGCTTGGGTAATCACCGGACCCAGCGCTGGGACCTTGCCCGTTTTACGGTTTAA	ATGRLGNHRTQRWDLARFTV*	-1.133	0.000463	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26815	PTFCALAHLTS			11	SLAY-screened peptide P5165			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACCTTCTGCGCGCTTGCCCACCTTACCTCTTAGTCCACTCTGCAGGGCGTTCTGTATTAA	PTFCALAHLTS*STLQGVLY*	-1.133	0.040976	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26816	FNPCGSCTPFTCWLPRSYK			19	SLAY-screened peptide P5166			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAACCCGTGTGGCTCTTGTACTCCCTTTACGTGTTGGCTCCCCCGGTCTTACAAGTAGTAA	FNPCGSCTPFTCWLPRSYK**	-1.133	0.009782	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26817	WLQIRIRGDP			10	SLAY-screened peptide P5167			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCTTCAGATCCGGATCAGGGGCGATCCTTAGGCGCACTAGCCTGAGCTGACTTAGGTCTAA	WLQIRIRGDP*AH*PELT*V*	-1.132	0.026727	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26818	STNTNDWFSHNTPPTVVSD			19	SLAY-screened peptide P5168			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTACCAACACCAATGACTGGTTTTCCCATAATACGCCCCCCACTGTTGTGAGCGATTAACTG	STNTNDWFSHNTPPTVVSD*L	-1.132	0.001499	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26819	PLQSPYFCVSPSELMSQYRI			20	SLAY-screened peptide P5169			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTCAGTCCCCGTATTTTTGTGTGAGCCCCTCTGAGCTCATGTCCCAGTACCGTATTTAA	PLQSPYFCVSPSELMSQYRI*	-1.132	0.000684	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26820	RLKYFSPL			8	SLAY-screened peptide P5170			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTTAAGTACTTTAGCCCTTTGTAGAACAAGGACTGTTACTATGATGCCCACCATCCTTAA	RLKYFSPL*NKDCYYDAHHP*	-1.131	0.018192	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26821	FKDACPGTPYSYPIIIVAAQ			20	SLAY-screened peptide P5171			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAAGGATGCCTGCCCGGGTACGCCCTATTCCTATCCCATTATTATCGTTGCTGCTCAGTAA	FKDACPGTPYSYPIIIVAAQ*	-1.131	0.000697	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26822	PMLCYYSPITNGGKSCFVFVN			21	SLAY-screened peptide P5172			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATGCTCTGCTATTATAGTCCGATCACCAATGGCGGTAAGAGTTGCTTCGTATTTGTTAAC	PMLCYYSPITNGGKSCFVFVN	-1.131	0.021789	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26823	YCATTYSDACSIFPHATA			18	SLAY-screened peptide P5173			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTGTGCCACCACTTACTCCGATGCGTGCTCTATTTTCCCTCACGCCACCGCCTAGAATTAA	YCATTYSDACSIFPHATA*N*	-1.131	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26824	PFHYLLI			7	SLAY-screened peptide P5174			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTCCATTATCTCCTGATTTAGTTTTGTGAGACCCATACGTCCAACCAGCAGACATTTAAC	PFHYLLI*FCETHTSNQQTFN	-1.131	6.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26825	ALPMFTVSCLISNAVLHRRS			20	SLAY-screened peptide P5175			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCTCCCCATGTTTACCGTCTCGTGTCTTATCTCCAACGCCGTTTTGCACAGGCGGTCCTAA	ALPMFTVSCLISNAVLHRRS*	-1.131	9.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26826	ILWLRLLRHFVFSKTIHTIP			20	SLAY-screened peptide P5176			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCTGTGGCTGCGGCTCCTTCGCCACTTCGTTTTCTCCAAGACCATTCATACCATTCCGTAA	ILWLRLLRHFVFSKTIHTIP*	-1.131	0.039884	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26827	RHPLLTWCDHHMCPCAYWLN			20	SLAY-screened peptide P5177			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCATCCCTTGCTTACCTGGTGCGATCACCATATGTGTCCCTGTGCTTACTGGCTTAACTAA	RHPLLTWCDHHMCPCAYWLN*	-1.131	3.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26828	PKTFTPYATGSDCFSIDYIV			20	SLAY-screened peptide P5178			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAAGACTTTTACGCCGTATGCTACCGGTTCTGACTGCTTCAGTATCGATTATATCGTTTAA	PKTFTPYATGSDCFSIDYIV*	-1.131	0.003917	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26829	THYYFHLFTTLTGCVESASD			20	SLAY-screened peptide P5179			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCATTATTACTTCCATCTTTTCACGACCCTTACTGGTTGCGTCGAGTCGGCGTCGGACTAA	THYYFHLFTTLTGCVESASD*	-1.131	0.03453	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26830	NCLTHNPGASHSMTPLWHAL			20	SLAY-screened peptide P5180			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTGCCTCACCCATAATCCCGGCGCTTCCCATAGTATGACGCCCCTTTGGCATGCTCTCTAA	NCLTHNPGASHSMTPLWHAL*	-1.131	0.010594	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26831	PVGLDASEIAS			11	SLAY-screened peptide P5181			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGTTGGCCTCGACGCTTCCGAGATTGCTAGCTAGCTTTTCTAGATTGCCCACTCCAGTTAA	PVGLDASEIAS*LF*IAHSS*	-1.131	0.02335	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26832	AHCLRSHNSPSYLPAHVHRA			20	SLAY-screened peptide P5182			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCACTGTCTTCGTTCGCACAATTCTCCGTCTTACCTCCCGGCTCACGTCCATCGGGCCTAA	AHCLRSHNSPSYLPAHVHRA*	-1.13	0.013238	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26833	ELTPCLL			7	SLAY-screened peptide P5183			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCTTACCCCTTGCCTCTTGTAGAGCCTCATTGGCTGCTCTGCTGGCTGCTGTTCCGCCTAA	ELTPCLL*SLIGCSAGCCSA*	-1.13	0.000586	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26834	LPTPCTLHPYSRALLVNLTN			20	SLAY-screened peptide P5184			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCCACCCCTTGCACGCTGCATCCCTACAGTCGGGCTTTGCTCGTGAATCTCACCAACTAA	LPTPCTLHPYSRALLVNLTN*	-1.13	0.033112	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26835	TYPACLYVHSNYIMTMPVYT			20	SLAY-screened peptide P5185			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTATCCCGCTTGTCTCTATGTTCACAGCAACTATATCATGACCATGCCTGTCTATACCTAA	TYPACLYVHSNYIMTMPVYT*	-1.13	4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26836	TD			2	SLAY-screened peptide P5186			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGACTAGTGGGATTCGTATCCCATCGGACTGCAATAAGACGCGTCCCCGTTTTGTGTAACT	TD*WDSYPIGLQ*DASPFCVT	-1.13	0.001014	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26837	RAIDGPKGKLHTYIYATGTA			20	SLAY-screened peptide P5187			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCGATTGATGGTCCTAAGGGTAAGCTCCACACTTACATCTATGCCACGGGTACCGCTTAA	RAIDGPKGKLHTYIYATGTA*	-1.13	6e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26838	TGDILFHFFYQPTYSLFNLW			20	SLAY-screened peptide P5188			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGGTGATATTCTCTTCCATTTCTTCTATCAGCCGACTTATTCTCTCTTTAATCTGTGGTAA	TGDILFHFFYQPTYSLFNLW*	-1.129	0.038349	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26839	PYATFLPPLISAATCVACNP			20	SLAY-screened peptide P5189			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTACGCCACTTTTCTCCCGCCTCTTATCTCGGCGGCTACTTGCGTCGCTTGCAATCCCTAA	PYATFLPPLISAATCVACNP*	-1.129	0.006035	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26840	RLTSLYVPSPPFLFSVSYQR			20	SLAY-screened peptide P5190			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTTGACGTCGCTTTATGTTCCCAGCCCCCCCTTCCTGTTTTCTGTGTCTTATCAGCGTTAA	RLTSLYVPSPPFLFSVSYQR*	-1.129	0.013835	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26841	PSLGRAPPTSAYHVPDTPIP			20	SLAY-screened peptide P5191			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCGCTGGGGCGCGCGCCGCCGACTAGTGCTTATCATGTTCCGGACACTCCTATTCCCTAA	PSLGRAPPTSAYHVPDTPIP*	-1.129	0.007639	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26842	ASSSDTCTHD			10	SLAY-screened peptide P5192			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTCTTCTTCCGACACGTGTACGCATGATTAGACGTACAACGACGCCAATCGCCGCGTCTAA	ASSSDTCTHD*TYNDANRRV*	-1.128	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26843	TWSLRDPYQANPFTVRCFY			19	SLAY-screened peptide P5193			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGTGGAGCCTGAGGGACCCGTATCAGGCTAATCCCTTTACCGTTCGTTGTTTCTACTAACTG	TWSLRDPYQANPFTVRCFY*L	-1.128	0.006612	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26844	CVLNTCRRRVVYPANTNSNY			20	SLAY-screened peptide P5194			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGTCCTTAACACCTGTCGTCGGAGGGTTGTGTACCCCGCTAATACGAACAGCAATTATTAA	CVLNTCRRRVVYPANTNSNY*	-1.128	5.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26845	PFADEYVLPHGFIPYPQTPL			20	SLAY-screened peptide P5195			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTTCGCCGACGAGTACGTTCTGCCTCATGGTTTCATTCCCTATCCTCAGACTCCCCTCTAA	PFADEYVLPHGFIPYPQTPL*	-1.127	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26846	LFPCWAYHLHNNHFLNMTLY			20	SLAY-screened peptide P5196			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTTCCCCTGCTGGGCCTATCACCTCCATAATAACCATTTTCTTAATATGACTTTGTACTAA	LFPCWAYHLHNNHFLNMTLY*	-1.127	0.001233	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26847	YHSPFCTPFQLLDARVASPA			20	SLAY-screened peptide P5197			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCACTCTCCTTTCTGCACGCCTTTTCAGCTTCTGGATGCTAGGGTTGCGTCCCCTGCGTAA	YHSPFCTPFQLLDARVASPA*	-1.127	0.000532	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26848	LRPRRYFNLLFGRRRDLKRS			20	SLAY-screened peptide P5198			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCGCCCCCGTCGCTATTTCAATTTGTTGTTCGGTAGGCGCCGTGACCTTAAGCGGAGCTAA	LRPRRYFNLLFGRRRDLKRS*	-1.126	0.016259	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26849	VTSPPTYQTCFVCWFSVLCN			20	SLAY-screened peptide P5199			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCACCAGCCCCCCCACGTATCAGACTTGCTTCGTTTGTTGGTTTTCTGTCTTGTGCAACTAA	VTSPPTYQTCFVCWFSVLCN*	-1.126	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26850	PGEQHSLMLSRVLLAALRYVM			21	SLAY-screened peptide P5200			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGTGAGCAGCATTCCTTGATGTTATCACGGGTCTTGTTAGCAGCTCTACGCTATGTAATG	PGEQHSLMLSRVLLAALRYVM	-1.126	0.000218	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26851	HACLLTNYHTLNAVTWMRKP			20	SLAY-screened peptide P5201			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCCTGCCTTCTTACTAATTACCACACGCTTAACGCCGTTACCTGGATGCGCAAGCCTTAA	HACLLTNYHTLNAVTWMRKP*	-1.126	0.00178	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26852	VRRIDDPGCQWNHYSSAG			18	SLAY-screened peptide P5202			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCGGCGTATCGATGACCCTGGGTGCCAGTGGAACCACTATTCTTCCGCTGGTTAGCTGTAA	VRRIDDPGCQWNHYSSAG*L*	-1.126	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26853	PLIAPHLLYCNHN			13	SLAY-screened peptide P5203			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTATTGCTCCTCACCTCTTGTATTGCAACCATAATTGACCTACCATCATAGTGCCTAAC	PLIAPHLLYCNHN*PTIIVPN	-1.126	8.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26854	NNPSCDCSFFPKLLFIPCLA			20	SLAY-screened peptide P5204			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACAACCCGTCTTGCGATTGTAGTTTCTTCCCTAAGCTGTTGTTCATTCCTTGCCTCGCCTAA	NNPSCDCSFFPKLLFIPCLA*	-1.125	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26855	PDEINCSLQTRTLNVHIYSH			20	SLAY-screened peptide P5205			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGACGAGATTAACTGTTCTCTCCAGACTCGTACGCTTAATGTTCATATCTACAGTCATTAA	PDEINCSLQTRTLNVHIYSH*	-1.125	7.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26856	PSPDYTCPNYASSMFHLYSI			20	SLAY-screened peptide P5206			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGTCCGGACTATACGTGCCCGAATTACGCCAGCTCTATGTTTCATCTTTACAGCATCTAA	PSPDYTCPNYASSMFHLYSI*	-1.125	0.001057	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26857	SDTCAVASA			9	SLAY-screened peptide P5207			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGATACTTGTGCTGTCGCGTCCGCGTAGTCGAGTGTTAACGTCACCCATAGTCGTTTTTAA	SDTCAVASA*SSVNVTHSRF*	-1.125	0.022835	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26858	RFPFWMSFAVSKCTRHSYKY			20	SLAY-screened peptide P5208			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTTTCCCTTTTGGATGTCGTTCGCGGTCTCCAAGTGTACTCGGCATAGCTATAAGTACTAA	RFPFWMSFAVSKCTRHSYKY*	-1.125	0.024258	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26859	PT			2	SLAY-screened peptide P5209			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACTTAGAGCCTTACCCTCCGGCTGCGGCCCACGACCGTCAGCCATCCTATCAAGGACTAA	PT*SLTLRLRPTTVSHPIKD*	-1.124	0.004099	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26860	SGVHFSPSTRLSGDPNGAVS			20	SLAY-screened peptide P5210			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGGTGTCCACTTTAGCCCCTCCACGCGCCTCTCCGGGGACCCCAACGGTGCCGTCTCCTAA	SGVHFSPSTRLSGDPNGAVS*	-1.124	0.000366	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26861	STHHKGNHDIYPIYVHH			17	SLAY-screened peptide P5211			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCACTCACCACAAGGGTAATCATGACATCTACCCTATTTATGTTCACCATTAGCACTGCTAA	STHHKGNHDIYPIYVHH*HC*	-1.124	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26862	SDV			3	SLAY-screened peptide P5212			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGACGTTTAGGCCCCCATCAATTGGGTTCCTAACTCTTGTCATGACTGCGACGCGTACTAA	SDV*APINWVPNSCHDCDAY*	-1.124	0.000682	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26863	LPSIHTIS			8	SLAY-screened peptide P5213			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCGTCGATTCATACTATCTCTTAGGAGTCCTTCCTCAATAGCCGGGTCTCCTGTCTCTAA	LPSIHTIS*ESFLNSRVSCL*	-1.123	0.008227	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26864	YCHTPAQFYPLR			12	SLAY-screened peptide P5214			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTGCCATACCCCCGCGCAGTTTTATCCGTTGCGGTAGTCTCTCCTCACTGCCGGCAGCTAA	YCHTPAQFYPLR*SLLTAGS*	-1.123	0.024152	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26865	RDLPSNCCLIIYPLVSTGDW			20	SLAY-screened peptide P5215			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGACCTCCCCAGCAACTGTTGCCTTATCATTTATCCGCTTGTCTCTACTGGCGACTGGTAA	RDLPSNCCLIIYPLVSTGDW*	-1.123	0.002815	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26866	SVSAKWRPVLLFWT			14	SLAY-screened peptide P5216			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGTGTCCGCCAAGTGGCGGCCTGTGCTGCTCTTTTGGACGTAGGCCCCTACCTAGTGTTAA	SVSAKWRPVLLFWT*APT*C*	-1.123	0.01258	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26867	PRVYIATMTESYLLMVSRCY			20	SLAY-screened peptide P5217			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGCGTTTACATCGCCACCATGACCGAGAGCTATCTGCTTATGGTCTCTCGCTGTTACTAA	PRVYIATMTESYLLMVSRCY*	-1.123	0.008061	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26868	ARSHPRATDHMLIFCMTYYP			20	SLAY-screened peptide P5218			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCGTTCTCACCCTCGTGCTACTGACCACATGCTGATCTTCTGCATGACTTACTATCCTTAA	ARSHPRATDHMLIFCMTYYP*	-1.122	0.002428	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26869	PLDMKSTLPRHGHYPHQSI			19	SLAY-screened peptide P5219			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCGATATGAAGTCCACCTTGCCTCGTCATGGTCATTATCCCCACCAGTCTATTTAGTAA	PLDMKSTLPRHGHYPHQSI**	-1.122	0.000673	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26870	PYCRGGNRSLPRTVFPYVPL			20	SLAY-screened peptide P5220			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTATTGCCGGGGCGGGAACAGGTCGCTGCCCAGGACCGTCTTTCCTTATGTTCCGCTTTAA	PYCRGGNRSLPRTVFPYVPL*	-1.122	0.015636	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26871	VSPILYSIGHKNPKYSTGYC			20	SLAY-screened peptide P5221			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTCTCCTATCCTTTACTCCATTGGTCACAAGAACCCCAAGTACTCGACGGGTTATTGTTAA	VSPILYSIGHKNPKYSTGYC*	-1.122	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26872	YCPLYLAANQIYI			13	SLAY-screened peptide P5222			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTGCCCCCTGTACCTGGCTGCCAACCAGATTTACATTTAGCACTAGCTTTTCTATAACTAA	YCPLYLAANQIYI*H*LFYN*	-1.121	6.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26873	SRNRSACAFKQPDTMAKSYY			20	SLAY-screened peptide P5223			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGAGGAACCGCAGTGCCTGCGCCTTCAAGCAGCCCGATACGATGGCTAAGAGTTACTATTAA	SRNRSACAFKQPDTMAKSYY*	-1.121	0.00149	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26874	VLTTVVVNCPWDRG			14	SLAY-screened peptide P5224			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCCTCACGACCGTTGTCGTTAACTGTCCCTGGGATCGCGGCTAGGTTGTGACGGGCAGTTAA	VLTTVVVNCPWDRG*VVTGS*	-1.121	0.000213	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26875	SQVILCS			7	SLAY-screened peptide P5225			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCAGGTTATCCTCTGTTCGTAGTCTCTCAGCTATTACATTCACAACTACAATAAGCGTTAA	SQVILCS*SLSYYIHNYNKR*	-1.121	0.000173	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26876	FAVHVTHRAQPLSPQGLYLH			20	SLAY-screened peptide P5226			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGCCGTTCATGTTACTCACAGGGCCCAGCCGCTCTCTCCCCAGGGCCTTTATCTTCATTAA	FAVHVTHRAQPLSPQGLYLH*	-1.121	0.003176	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26877	CQTRSANLLTIFIIVVLLRRN			21	SLAY-screened peptide P5227			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCAGACGCGTTCTGCCAACTTGCTCACAATTTTCATAATAGTTGTCCTACTGCGGCGTAAC	CQTRSANLLTIFIIVVLLRRN	-1.121	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26878	VPNCNTLIYYIG			12	SLAY-screened peptide P5228			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCCGAATTGCAATACCCTTATTTATTATATCGGCTAGCGTAACGGGAGGAAGGCTTATTAA	VPNCNTLIYYIG*RNGRKAY*	-1.121	0.003846	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26879	PTVTCQPIPLLAPVYSV			17	SLAY-screened peptide P5229			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACGGTCACTTGTCAGCCTATCCCCCTCTTGGCCCCCGTTTACTCTGTGTAGAAGACCTAA	PTVTCQPIPLLAPVYSV*KT*	-1.121	0.000197	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26880	CAHACPKCPESSCHGYNHPH			20	SLAY-screened peptide P5230			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCTCACGCCTGCCCTAAGTGTCCGGAGAGTAGTTGCCACGGCTATAACCATCCCCACTAA	CAHACPKCPESSCHGYNHPH*	-1.121	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26881	PNLSFGSDCFWYTAVASLTT			20	SLAY-screened peptide P5231			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAATCTCTCGTTTGGCAGCGATTGCTTTTGGTACACTGCCGTTGCCTCTCTTACCACCTAA	PNLSFGSDCFWYTAVASLTT*	-1.121	0.014679	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26882	MPRGNNHIGATTCEDQTQ			18	SLAY-screened peptide P5232			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCCTCGGGGTAACAACCATATTGGGGCGACGACTTGCGAGGACCAGACCCAGTAGATGTAA	MPRGNNHIGATTCEDQTQ*M*	-1.12	0.006701	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26883	PVRNPIIYYRVNIHFHRIKR			20	SLAY-screened peptide P5233			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGTGCGTAATCCTATCATTTATTACAGGGTTAACATTCACTTTCACCGTATTAAGCGCTAA	PVRNPIIYYRVNIHFHRIKR*	-1.12	0.000983	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26884	PTWPNTCWIPYHPASLGIDA			20	SLAY-screened peptide P5234			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACGTGGCCGAATACGTGCTGGATTCCCTACCACCCCGCTTCTCTCGGTATTGATGCTTAA	PTWPNTCWIPYHPASLGIDA*	-1.12	0.002406	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26885	HSPARGSCVDLYYSYCYYSC			20	SLAY-screened peptide P5235			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAGTCCGGCCCGGGGCAGTTGCGTTGATCTTTACTACTCGTATTGTTATTATAGCTGTTAA	HSPARGSCVDLYYSYCYYSC*	-1.12	0.028116	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26886	PLDLMFCSGPNTPHFPSPQK			20	SLAY-screened peptide P5236			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTGGACCTCATGTTCTGTAGCGGCCCGAATACCCCGCATTTCCCCAGTCCTCAGAAGTAA	PLDLMFCSGPNTPHFPSPQK*	-1.12	0.000709	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26887	LCTSFVFFRASWLNHICTDA			20	SLAY-screened peptide P5237			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTGCACTTCGTTTGTTTTCTTTCGGGCGTCTTGGCTTAATCATATCTGTACCGACGCCTAA	LCTSFVFFRASWLNHICTDA*	-1.12	0.000162	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26888	VAPYGPYTPTIPSSRLYILV			20	SLAY-screened peptide P5238			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGCGCCGTACGGGCCTTATACCCCGACCATCCCTTCGTCCCGTTTGTACATTCTTGTCTAA	VAPYGPYTPTIPSSRLYILV*	-1.119	0.001738	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26889	SNPACVAAIIYAKSLCTLTL			20	SLAY-screened peptide P5239			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCAACCCCGCGTGCGTGGCCGCCATCATCTACGCTAAGTCCCTCTGTACGCTTACTCTGTAA	SNPACVAAIIYAKSLCTLTL*	-1.118	0.000222	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26890	THPHLYAYRTKYGSYGHLKV			20	SLAY-screened peptide P5240			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCACCCCCATCTCTACGCTTATCGGACTAAGTATGGCTCTTACGGTCACCTGAAGGTGTAA	THPHLYAYRTKYGSYGHLKV*	-1.118	0.000169	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26891	STTHPFHDRDLCCSPTYLYL			20	SLAY-screened peptide P5241			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTACGACGCACCCTTTCCACGACCGGGATCTGTGCTGTAGCCCGACCTACTTGTATCTGTAA	STTHPFHDRDLCCSPTYLYL*	-1.118	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26892	SCPNTAFTFDYAQLFNPRRM			20	SLAY-screened peptide P5242			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTGCCCTAACACCGCGTTTACGTTCGACTACGCCCAGCTTTTTAACCCGCGGAGGATGTAA	SCPNTAFTFDYAQLFNPRRM*	-1.118	0.005193	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26893	PTRSNVI			7	SLAY-screened peptide P5243			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACCCGTTCTAACGTCATTTAGGCTGGCGGCTTGGTTAGTAGCAATTCTGGCCCCTTGTAA	PTRSNVI*AGGLVSSNSGPL*	-1.118	0.000404	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26894	FFVDGTNLSIDIPNQWYLTPN			21	SLAY-screened peptide P5244			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTTCGTTGACGGGACCAACCTCTCGATTGATATCCCTAATCAGTGGTACCTTACACCTAAC	FFVDGTNLSIDIPNQWYLTPN	-1.118	0.010996	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26895	VPACLLIPSFLLCSFSPGHT			20	SLAY-screened peptide P5245			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCCCGCTTGTCTTCTTATCCCCAGTTTCCTCCTGTGCAGCTTTTCTCCTGGGCATACTTAA	VPACLLIPSFLLCSFSPGHT*	-1.117	0.000212	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26896	AVIGTTTFALSLVIRTSSLAN			21	SLAY-screened peptide P5246			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGGGCGGTTATTGGAACGACAACATTTGCGTTAAGCCTGGTAATAAGGACCTCCTCTCTCGCTAAC	AVIGTTTFALSLVIRTSSLAN	-1.117	0.037433	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26897	RPQ			3	SLAY-screened peptide P5247			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCTCAGTAGCCTGCCCGGCCCAGTCCTCGTCTGGTTGTTTATGTGCGTTGCTTGATCTAA	RPQ*PARPSPRLVVYVRCLI*	-1.117	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26898	SRHGVSDTTDSMPNCWGYIV			20	SLAY-screened peptide P5248			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCGCCACGGTGTTAGCGATACTACCGATAGTATGCCCAATTGCTGGGGGTATATTGTCTAA	SRHGVSDTTDSMPNCWGYIV*	-1.117	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26899	LLPLVYNQRGRLSNSYAPGP			20	SLAY-screened peptide P5249			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCTGCCCTTGGTGTACAACCAGCGCGGCCGTCTCAGCAATAGTTATGCGCCTGGTCCCTAA	LLPLVYNQRGRLSNSYAPGP*	-1.117	0.003404	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26900	HS			2	SLAY-screened peptide P5250			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTCGTAGGATGCGACGTATAATTGCTTGCGTGTGCCGACTTATTACTCCACGCGGCGCTAA	HS*DATYNCLRVPTYYSTRR*	-1.117	0.001398	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26901	NTDTKVCKALSFWISIPCPFN			21	SLAY-screened peptide P5251			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACACGGACACCAAGGTGTGCAAAGCCCTGAGCTTCTGGATATCCATACCATGCCCCTTTAAC	NTDTKVCKALSFWISIPCPFN	-1.116	0.042087	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26902	SCYCPLTVFGFPFLA			15	SLAY-screened peptide P5252			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTGCTACTGCCCGCTTACTGTTTTCGGTTTCCCTTTCCTGGCGTAGTGGCCCATGGATTAA	SCYCPLTVFGFPFLA*WPMD*	-1.116	0.012563	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26903	YSPTCNSVYPLGLFPTWGHY			20	SLAY-screened peptide P5253			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTCTCCCACCTGTAACTCTGTCTATCCTCTGGGGCTTTTCCCGACGTGGGGTCACTATTAA	YSPTCNSVYPLGLFPTWGHY*	-1.116	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26904	SPHLPPPVRLYHELRLPHLC			20	SLAY-screened peptide P5254			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCCCCATTTGCCCCCGCCGGTTAGGCTGTATCACGAGCTCCGCCTTCCTCATTTGTGTTAA	SPHLPPPVRLYHELRLPHLC*	-1.116	0.00063	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26905	RHYPNTSWRRPSSRLS			16	SLAY-screened peptide P5255			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCACTACCCGAACACTAGCTGGCGGCGCCCTTCCAGCCGGTTGAGCTAGGCCGACAAGTAA	RHYPNTSWRRPSSRLS*ADK*	-1.115	0.021704	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26906	MIAFPNRIIQ			10	SLAY-screened peptide P5256			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGATCGCCTTTCCCAATCGCATCATCCAGTAGGCGTAGAACTATCATCGCAGTCTGGACTAA	MIAFPNRIIQ*A*NYHRSLD*	-1.115	0.002429	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26907	CNLYYDCPYYTYLRYRGVKC			20	SLAY-screened peptide P5257			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAATCTTTACTATGATTGTCCTTACTACACTTATCTTCGTTACCGTGGCGTCAAGTGTTAA	CNLYYDCPYYTYLRYRGVKC*	-1.115	0.005243	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26908	PANNAEFLGRLHVPDVFST			19	SLAY-screened peptide P5258			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCTAATAATGCAGAATTTCTCGGCCGTTTACACGTGCCTGATGTTTTCTCCACCTAACTG	PANNAEFLGRLHVPDVFST*L	-1.115	0.039769	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26909	LQGKKIPIYLCLYNDITKNR			20	SLAY-screened peptide P5259			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCAGGGGAAGAAGATCCCCATTTACCTTTGTCTCTATAACGATATTACCAAGAATCGGTAA	LQGKKIPIYLCLYNDITKNR*	-1.115	0.020883	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26910	GLYIHVPHDMYPLYKCHYIF			20	SLAY-screened peptide P5260			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGCTTTATATCCATGTCCCCCACGATATGTATCCTCTTTATAAGTGCCACTATATCTTTTAA	GLYIHVPHDMYPLYKCHYIF*	-1.114	0.016505	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26911	RFCPREGRAEGWLPRYQTT			19	SLAY-screened peptide P5261			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTTTTGCCCGCGTGAGGGTAGGGCCGAGGGTTGGCTGCCCAGGTATCAGACGACCTAGTAA	RFCPREGRAEGWLPRYQTT**	-1.114	0.012391	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26912	CSFNQWYSMFPYSAAYNSLK			20	SLAY-screened peptide P5262			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAGTTTTAACCAGTGGTATTCCATGTTTCCCTACTCCGCGGCCTATAACAGTCTTAAGTAA	CSFNQWYSMFPYSAAYNSLK*	-1.114	0.000771	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26913	CVPKLFTPNLVRSCPSTVCN			20	SLAY-screened peptide P5263			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGTCCCCAAGCTTTTCACCCCGAACCTTGTTCGTAGTTGTCCCAGCACTGTGTGTAACTAA	CVPKLFTPNLVRSCPSTVCN*	-1.114	0.000276	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26914	PQSNYYLISMSDPNYWIGNI			20	SLAY-screened peptide P5264			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCAGAGTAATTATTATCTGATTTCGATGTCCGATCCGAACTACTGGATTGGCAATATTTAA	PQSNYYLISMSDPNYWIGNI*	-1.114	0.044327	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26915	VMF			3	SLAY-screened peptide P5265			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCATGTTTTAGGACTCGTCTAGCACCCGGTGCTCTTCCTTTCCTATGATTCGTACCCTGTAA	VMF*DSSSTRCSSFPMIRTL*	-1.114	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26916	NRMVCYSTDNAPFVLACIQS			20	SLAY-screened peptide P5266			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACAGGATGGTGTGCTACAGTACGGATAACGCCCCCTTCGTGCTTGCCTGTATCCAGTCGTAA	NRMVCYSTDNAPFVLACIQS*	-1.113	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26917	RISPAAKENFGFYLNSILLA			20	SLAY-screened peptide P5267			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGATTTCCCCTGCCGCCAAGGAGAACTTTGGTTTTTATCTCAACTCCATTTTGCTTGCGTAA	RISPAAKENFGFYLNSILLA*	-1.113	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26918	PNLCLLTPLSAFWYTYPPIM			20	SLAY-screened peptide P5268			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAATCTTTGTTTGCTCACTCCCCTGAGCGCGTTTTGGTACACTTATCCCCCGATTATGTAA	PNLCLLTPLSAFWYTYPPIM*	-1.113	0.010302	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26919	QGCLHPPRGAY			11	SLAY-screened peptide P5269			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAAGGATGCCTACACCCTCCACGAGGCGCTTATTAAGTTTCAGACTTCTATCCACTAGTAACT	QGCLHPPRGAY*VSDFYPLVT	-1.113	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26920	PPYSRWYTWRYTDYNAIYSC			20	SLAY-screened peptide P5270			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTTACTCTCGCTGGTATACGTGGAGGTACACCGATTACAATGCTATTTACAGCTGCTAA	PPYSRWYTWRYTDYNAIYSC*	-1.113	9e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26921	LGYSRTP			7	SLAY-screened peptide P5271			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGGCTACTCTCGTACGCCCTAGAGTCTTCGCAACGCCTATTTCAATCTTCATAACTTCTAA	LGYSRTP*SLRNAYFNLHNF*	-1.112	0.036348	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26922	TTITDCCRYDNPCTLFKWRY			20	SLAY-screened peptide P5272			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGACCATTACCGACTGTTGTCGTTATGACAACCCGTGCACTTTGTTCAAGTGGCGCTATTAA	TTITDCCRYDNPCTLFKWRY*	-1.112	0.001325	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26923	DCAHNFSHPVYTNSI			15	SLAY-screened peptide P5273			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTGCGCCCATAACTTTTCTCATCCCGTTTACACGAATAGCATTTAGCCCTTCAATCTGTAA	DCAHNFSHPVYTNSI*PFNL*	-1.112	4.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26924	PPDSGFNIDPCYVLVYAQLL			20	SLAY-screened peptide P5274			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTGATTCGGGCTTCAACATCGATCCTTGTTACGTCCTTGTTTACGCGCAGCTGCTTTAA	PPDSGFNIDPCYVLVYAQLL*	-1.111	0.012139	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26925	ADVFAASGRDTWSYYIMLHH			20	SLAY-screened peptide P5275			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGATGTTTTTGCGGCCTCGGGCCGCGATACTTGGTCGTATTACATCATGTTGCACCATTAA	ADVFAASGRDTWSYYIMLHH*	-1.111	0.002053	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26926	PARATSLTPLVVYPVVRCLS			20	SLAY-screened peptide P5276			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGCTAGGGCTACGAGCCTCACCCCTCTTGTGGTGTATCCCGTCGTCCGCTGCCTGTCGTAA	PARATSLTPLVVYPVVRCLS*	-1.111	0.010638	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26927	HALSNMNIEASGNPFCIYIM			20	SLAY-screened peptide P5277			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGCGTTGTCTAATATGAACATCGAGGCTAGTGGTAACCCCTTTTGTATCTATATTATGTAA	HALSNMNIEASGNPFCIYIM*	-1.111	0.04568	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26928	LGSWGYSRCTN			11	SLAY-screened peptide P5278			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGGCTCGTGGGGGTACTCCCGCTGTACTAATTAGATTCCCAACTGTATTCAGTGTGGGTAA	LGSWGYSRCTN*IPNCIQCG*	-1.11	2.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26929	GCFNVSYQMANIHSAIY			17	SLAY-screened peptide P5279			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTGCTTCAACGTCAGTTATCAGATGGCTAACATTCACAGTGCTATTTATTAGATTCCTTAA	GCFNVSYQMANIHSAIY*IP*	-1.11	0.026751	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26930	YSPVHTTSGRMCININDWRTN			21	SLAY-screened peptide P5280			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTCCCCTGTGCACACGACGAGCGGTCGTATGTGCATCAACATCAACGATTGGAGAACTAAC	YSPVHTTSGRMCININDWRTN	-1.11	0.000368	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26931	ATQENVFLICPYYSY			15	SLAY-screened peptide P5281			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACTCAGGAGAACGTCTTTCTCATCTGTCCTTACTACTCGTACTAGACCTGTATTCATTAA	ATQENVFLICPYYSY*TCIH*	-1.11	0.002403	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26932	HHGTVCLSSPISSCNLPYIP			20	SLAY-screened peptide P5282			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCATGGCACTGTCTGTCTTAGCTCGCCGATCTCTAGTTGCAATCTTCCGTATATTCCTTAA	HHGTVCLSSPISSCNLPYIP*	-1.11	0.00878	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26933	QQISSTPALSTTTPNTGVSD			20	SLAY-screened peptide P5283			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCAGATCTCGTCCACCCCGGCTCTTTCTACCACCACCCCGAACACTGGGGTGAGTGACTAA	QQISSTPALSTTTPNTGVSD*	-1.11	0.000254	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26934	GPRRVVNSPAVECSAEHVSL			20	SLAY-screened peptide P5284			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCCCAGGCGTGTTGTGAATTCTCCTGCTGTGGAGTGCTCCGCTGAGCACGTTAGCCTTTAA	GPRRVVNSPAVECSAEHVSL*	-1.11	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26935	TLYSHQPLTFYSPSVCLVYF			20	SLAY-screened peptide P5285			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTGTACAGTCATCAGCCTCTCACGTTCTACTCGCCCTCTGTTTGCCTTGTGTACTTTTAA	TLYSHQPLTFYSPSVCLVYF*	-1.11	0.002477	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26936	SKPPQSDSVMFMLDPISCFP			20	SLAY-screened peptide P5286			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTAAGCCCCCTCAGAGCGATTCTGTGATGTTTATGCTGGACCCGATTAGCTGTTTTCCGTAA	SKPPQSDSVMFMLDPISCFP*	-1.11	0.00062	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26937	ADRRVVLPHTPNCADGPPPA			20	SLAY-screened peptide P5287			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGACCGGCGCGTCGTTCTTCCGCACACGCCCAATTGCGCGGATGGGCCCCCCCCTGCCTAA	ADRRVVLPHTPNCADGPPPA*	-1.109	0.043025	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26938	PRVLPTPFGMRRSHRRRFHL			20	SLAY-screened peptide P5288			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGGGTGCTTCCCACTCCTTTTGGCATGCGCCGGTCTCACAGGCGTAGGTTCCACCTGTAA	PRVLPTPFGMRRSHRRRFHL*	-1.109	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26939	RPYRVAHSALVLHCNSWDRP			20	SLAY-screened peptide P5289			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCTTATCGCGTTGCTCACTCGGCTCTTGTCCTCCACTGTAACTCGTGGGATCGCCCGTAA	RPYRVAHSALVLHCNSWDRP*	-1.109	0.004169	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26940	TCTRRQYTTFMFPTV			15	SLAY-screened peptide P5290			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTGTACCAGGCGTCAGTACACGACGTTTATGTTCCCTACTGTCTAGCGGCTAGACGTAACT	TCTRRQYTTFMFPTV*RLDVT	-1.109	0.035998	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26941	RGCALDTLNIV			11	SLAY-screened peptide P5291			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGGCTGCGCTCTTGATACTCTCAACATCGTTTAGTACAAGCGCCCTAATACTATTAATTAA	RGCALDTLNIV*YKRPNTIN*	-1.109	0.002935	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26942	LGGYACPYCHWFHITRNDCY			20	SLAY-screened peptide P5292			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGGGGGCTACGCTTGTCCGTACTGTCATTGGTTCCATATCACGCGCAACGATTGTTACTAA	LGGYACPYCHWFHITRNDCY*	-1.109	0.013038	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26943	THLWSYPKLIPTLLCMFCRH			20	SLAY-screened peptide P5293			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCATCTGTGGAGTTACCCGAAGCTCATCCCGACTCTCTTGTGCATGTTCTGTCGGCACTAA	THLWSYPKLIPTLLCMFCRH*	-1.108	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26944	PRGHGLLLRDCIHDFPIYRT			20	SLAY-screened peptide P5294			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAGGGGCCATGGGCTTCTGCTTCGGGACTGTATTCACGATTTTCCTATTTATCGCACGTAA	PRGHGLLLRDCIHDFPIYRT*	-1.108	0.000327	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26945	PCIYAFPANYTHIIVHLART			20	SLAY-screened peptide P5295			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGCATCTACGCCTTCCCGGCTAATTACACTCACATCATCGTTCACTTGGCCCGTACGTAA	PCIYAFPANYTHIIVHLART*	-1.108	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26946	HESTYMQDIYSPYTPFLQQC			20	SLAY-screened peptide P5296			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGAGTCCACTTATATGCAGGATATCTATTCCCCCTATACTCCTTTCCTTCAGCAGTGCTAA	HESTYMQDIYSPYTPFLQQC*	-1.108	0.000397	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26947	AHGHSLDLLYIIVMFALYCY			20	SLAY-screened peptide P5297			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCACGGCCACTCTCTTGACCTTCTCTACATCATCGTCATGTTCGCCTTGTACTGTTATTAA	AHGHSLDLLYIIVMFALYCY*	-1.108	0.000316	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26948	ETPHLAALLAYLLIVSPHAL			20	SLAY-screened peptide P5298			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGACCCCCCATCTGGCCGCCCTGTTGGCTTATTTGCTGATTGTCTCGCCCCACGCCCTCTAA	ETPHLAALLAYLLIVSPHAL*	-1.108	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26949	HLVSISL			7	SLAY-screened peptide P5299			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCTTGTCAGTATTTCCCTCTAGAATTTTACGCAGGTCCTGCTGAACTCGTCTCTTTCCTAA	HLVSISL*NFTQVLLNSSLS*	-1.108	0.012185	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26950	RSFAALGGYLPCYYLGSDHR			20	SLAY-screened peptide P5300			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCTTTTGCGGCGCTGGGCGGTTACCTTCCGTGCTATTACCTCGGTTCTGACCACCGCTAA	RSFAALGGYLPCYYLGSDHR*	-1.108	0.007761	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26951	ASTLRFPTHADSKV			14	SLAY-screened peptide P5301			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTCGACCCTGCGGTTTCCTACTCACGCCGATTCGAAGGTCTAGGAGACGGATAGTTTCTAA	ASTLRFPTHADSKV*ETDSF*	-1.108	0.001194	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26952	RRKRWLPALALRSLLMLVGVN			21	SLAY-screened peptide P5302			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGTAAACGTTGGTTACCGGCGCTCGCTCTCCGGAGTTTGCTAATGTTAGTAGGTGTTAAC	RRKRWLPALALRSLLMLVGVN	-1.107	0.006193	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26953	PSPCTTPLINGVPVHLVSRR			20	SLAY-screened peptide P5303			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTCTCCGTGCACGACTCCCCTGATTAATGGTGTCCCGGTCCACCTTGTCAGCCGTCGGTAA	PSPCTTPLINGVPVHLVSRR*	-1.107	0.014638	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26954	IVALHSNSHLRRYFHDSNID			20	SLAY-screened peptide P5304			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGTCGCGTTGCACAGCAATTCTCATCTTAGGCGCTATTTCCACGACTCTAACATTGACTAA	IVALHSNSHLRRYFHDSNID*	-1.107	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26955	TTATIIGY			8	SLAY-screened peptide P5305			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGACCGCTACTATCATCGGGTATTAGACGCGCCAGGGGTTTAACGGGCTCCGTCTCTATTAA	TTATIIGY*TRQGFNGLRLY*	-1.107	0.000199	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26956	TAHVKTHPSIL			11	SLAY-screened peptide P5306			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGCTCATGTTAAGACTCATCCTTCGATTCTCTAGCATCACTCCCGCACTATTCCTAGGTAA	TAHVKTHPSIL*HHSRTIPR*	-1.106	0.001717	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26957	SFNCPFMPVKRTPLIERVIY			20	SLAY-screened peptide P5307			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTTCAATTGTCCGTTCATGCCGGTGAAGAGGACTCCGCTCATTGAGCGCGTTATCTATTAA	SFNCPFMPVKRTPLIERVIY*	-1.106	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26958	SDPCNSIYPYTPLVFFTVRT			20	SLAY-screened peptide P5308			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGACCCGTGTAACAGCATCTATCCGTATACCCCTCTTGTTTTCTTCACTGTTAGGACCTAA	SDPCNSIYPYTPLVFFTVRT*	-1.106	0.011697	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26959	NRDASYPADISAVSEYSGHS			20	SLAY-screened peptide P5309			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCGGGATGCGTCCTACCCCGCGGACATTTCGGCTGTTTCGGAGTACTCCGGGCACTCCTAA	NRDASYPADISAVSEYSGHS*	-1.106	0.00873	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26960	ASSIILDK			8	SLAY-screened peptide P5310			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTCTTCGATTATTTTGGATAAGTAGTATACGAGGACGTTTGCGACTTGCGTGGCGTCCTAA	ASSIILDK*YTRTFATCVAS*	-1.106	0.001997	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26961	NTSAWTPCDFATHSTLRPFP			20	SLAY-screened peptide P5311			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACACGAGTGCCTGGACTCCTTGCGATTTTGCTACGCACTCCACCCTTCGGCCGTTCCCCTAA	NTSAWTPCDFATHSTLRPFP*	-1.106	0.002782	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26962	GTSWHSWHPIELTYGHCHLY			20	SLAY-screened peptide P5312			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGACCAGCTGGCACTCTTGGCACCCCATTGAGTTGACGTATGGTCATTGTCACTTGTATTAA	GTSWHSWHPIELTYGHCHLY*	-1.106	0.001316	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26963	CNWPPVSLYHTPFLYAVHLD			20	SLAY-screened peptide P5313			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAATTGGCCGCCCGTTTCGCTGTATCATACCCCTTTTCTTTACGCTGTTCATCTGGATTAA	CNWPPVSLYHTPFLYAVHLD*	-1.106	0.0001	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26964	NRSTITSIHYMSSMPHPVIT			20	SLAY-screened peptide P5314			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCGGTCCACTATTACGAGCATCCATTACATGTCGTCTATGCCCCACCCGGTGATTACCTAA	NRSTITSIHYMSSMPHPVIT*	-1.106	0.013142	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26965	RYSIPNHLYVYLSNTSGRLR			20	SLAY-screened peptide P5315			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTATTCTATCCCTAACCATTTGTACGTTTACCTCTCTAATACTTCCGGTAGGCTTCGTTAA	RYSIPNHLYVYLSNTSGRLR*	-1.105	0.000183	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26966	GARYNSPHTWAPVNTLYAKN			20	SLAY-screened peptide P5316			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGGCTAGGTATAACTCTCCTCACACTTGGGCCCCTGTCAACACGCTTTATGCCAAGAACTAA	GARYNSPHTWAPVNTLYAKN*	-1.105	2.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26967	CLSRMPYFVYPRIFNYTLSTC			21	SLAY-screened peptide P5317			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTGTCCCGTATGCCTTATTTCGTTTATCCTCGTATTTTTAACTACACGTTGTCGACCTGC	CLSRMPYFVYPRIFNYTLSTC	-1.105	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26968	LYSVRTGTPQLTLMMPCDTY			20	SLAY-screened peptide P5318			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTATAGTGTGCGTACCGGCACCCCCCAGCTTACTCTTATGATGCCGTGCGACACGTACTAA	LYSVRTGTPQLTLMMPCDTY*	-1.104	0.047494	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26969	LSSSNASSPPCMWSQHMYFP			20	SLAY-screened peptide P5319			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCAGTTCCAGCAACGCTTCTTCGCCCCCCTGTATGTGGTCTCAGCACATGTACTTTCCTTAA	LSSSNASSPPCMWSQHMYFP*	-1.104	8.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26970	LWVCLAVRHQSIVTIAQTWS			20	SLAY-screened peptide P5320			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTGGGTTTGCCTTGCTGTGCGTCACCAGTCCATTGTTACGATCGCGCAGACCTGGTCTTAA	LWVCLAVRHQSIVTIAQTWS*	-1.104	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26971	WRRALELGLHLAILYPLPIP			20	SLAY-screened peptide P5321			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGAGGAGGGCCCTCGAGCTTGGCCTCCATTTGGCGATTCTTTACCCTTTGCCTATCCCCTAA	WRRALELGLHLAILYPLPIP*	-1.103	0.0383	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26972	TSPAVSINNHFLTINHMTAM			20	SLAY-screened peptide P5322			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAGCCCTGCCGTCAGCATTAATAATCACTTTCTCACTATCAACCATATGACCGCCATGTAA	TSPAVSINNHFLTINHMTAM*	-1.103	0.015287	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26973	DDKRSEPHFTSNISRALLLAN			21	SLAY-screened peptide P5323			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGACAAGAGGTCCGAGCCCCACTTTACCTCGAACATTAGCCGAGCGCTACTCCTAGCTAAC	DDKRSEPHFTSNISRALLLAN	-1.103	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26974	ACPPARSWRMLPNALISSRV			20	SLAY-screened peptide P5324			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTGTCCCCCCGCCCGCTCTTGGCGCATGCTCCCCAACGCCTTGATTTCCTCGAGGGTGTAA	ACPPARSWRMLPNALISSRV*	-1.103	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26975	PHQGYFHHHCHPPRYQDYSL			20	SLAY-screened peptide P5325			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCACCAGGGCTATTTCCATCATCACTGCCACCCGCCTCGCTACCAGGATTACTCTTTGTAA	PHQGYFHHHCHPPRYQDYSL*	-1.103	0.000646	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26976	GPFGFWLRPCHYHPLFSDHL			20	SLAY-screened peptide P5326			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTCCGTTCGGGTTCTGGCTTCGCCCGTGCCACTACCACCCTCTTTTTTCCGATCACCTGTAA	GPFGFWLRPCHYHPLFSDHL*	-1.103	0.026346	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26977	PTSAYLPHEGRWSLRSSCLIN			21	SLAY-screened peptide P5327			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACTTCTGCTTATCTCCCCCATGAGGGTCGGTGGTCTCTTCGTTCGAGTTGCCTCATTAAC	PTSAYLPHEGRWSLRSSCLIN	-1.102	2.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26978	TPHQGTCYTNYQIPNYQSAS			20	SLAY-screened peptide P5328			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCGCACCAGGGTACCTGTTACACTAATTACCAGATCCCTAACTATCAGAGCGCGTCTTAA	TPHQGTCYTNYQIPNYQSAS*	-1.102	0.045084	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26979	ARMMNLLYQYSTDWLMCTDF			20	SLAY-screened peptide P5329			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCGTATGATGAACCTGCTCTACCAGTATTCGACCGATTGGTTGATGTGCACTGACTTCTAA	ARMMNLLYQYSTDWLMCTDF*	-1.102	0.047413	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26980	TSYHDLRRSLQSRCARIRLAL			21	SLAY-screened peptide P5330			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTCTTACCACGATCTTAGGCGCTCCTTGCAGAGTCGGTGCGCTCGTATTCGGTTGGCATTA	TSYHDLRRSLQSRCARIRLAL	-1.102	0.016807	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26981	TSAPSPPIDLNSLCQTIALA			20	SLAY-screened peptide P5331			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGAGTGCCCCGAGCCCCCCTATCGATTTGAACAGCTTGTGCCAGACCATCGCCCTCGCTTAA	TSAPSPPIDLNSLCQTIALA*	-1.102	0.020753	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26982	FCPGNYRLLCSSCPTYAYAI			20	SLAY-screened peptide P5332			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTGTCCTGGTAATTATCGTTTGCTCTGTTCTTCGTGTCCCACGTATGCGTACGCCATTTAA	FCPGNYRLLCSSCPTYAYAI*	-1.102	0.010791	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26983	HTSRTLTSSSHRAVCLSSSS			20	SLAY-screened peptide P5333			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACGTCTAGGACCCTCACCTCCAGTAGTCACCGCGCTGTTTGCCTTTCCTCGTCTTCTTAA	HTSRTLTSSSHRAVCLSSSS*	-1.102	0.000689	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26984	PPTCAPGYLP			10	SLAY-screened peptide P5334			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCACCTGCGCCCCCGGTTACTTGCCCTAGACTCCCGCTGTTCCGCATACTGTTGGTTAA	PPTCAPGYLP*TPAVPHTVG*	-1.102	0.000781	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26985	PWLDHVPVTSEFCYCGCPEY			20	SLAY-screened peptide P5335			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGGTTGGATCATGTTCCCGTTACGTCTGAGTTCTGTTATTGCGGTTGTCCTGAGTACTAA	PWLDHVPVTSEFCYCGCPEY*	-1.101	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26986	QGPSLFRLLELFFSRVKEIK			20	SLAY-screened peptide P5336			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGGCCCGAGCCTTTTCCGCCTTCTTGAGCTGTTTTTCTCGAGGGTTAAGGAGATTAAGTAA	QGPSLFRLLELFFSRVKEIK*	-1.101	0.008371	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26987	YTCPTMPPYHLITLYSNRKL			20	SLAY-screened peptide P5337			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATACCTGTCCGACTATGCCTCCTTACCATCTTATCACTCTTTACTCCAATAGGAAGCTCTAA	YTCPTMPPYHLITLYSNRKL*	-1.101	0.025359	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26988	PTCNSPQHLVTTPRYLLIEY			20	SLAY-screened peptide P5338			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGACGTGCAACAGCCCTCAGCATTTGGTCACCACTCCTAGGTATCTTCTCATCGAGTATTAA	PTCNSPQHLVTTPRYLLIEY*	-1.101	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26989	HRTALLPNNDLTCLPNRITP			20	SLAY-screened peptide P5339			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCGGACGGCTCTTCTGCCTAACAATGATCTTACCTGCTTGCCGAACCGCATCACTCCGTAA	HRTALLPNNDLTCLPNRITP*	-1.1	0.000429	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26990	TASTSAFDVYTPSSPSIRES			20	SLAY-screened peptide P5340			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGCTTCCACTTCGGCCTTTGATGTTTACACTCCTAGTAGCCCCTCGATCCGCGAGTCTTAA	TASTSAFDVYTPSSPSIRES*	-1.1	0.004574	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26991	SVRPK			5	SLAY-screened peptide P5341			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGTTCGCCCGAAGTAGCGTATCCCTACTTATCCGGGGTGGTTTACTGCCATCTTTCGTTAA	SVRPK*RIPTYPGWFTAIFR*	-1.1	0.000627	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26992	SNCRPGQPQRMTLVYV			16	SLAY-screened peptide P5342			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCAACTGCCGCCCCGGCCAGCCGCAGCGGATGACCCTTGTGTACGTGTAGCGCCCCGCGTAA	SNCRPGQPQRMTLVYV*RPA*	-1.099	0.034273	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26993	SHRPGACSAILHFAYPHLIM			20	SLAY-screened peptide P5343			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCATCGCCCTGGTGCCTGTAGCGCTATCCTCCACTTTGCGTATCCTCATCTGATTATGTAA	SHRPGACSAILHFAYPHLIM*	-1.098	0.011321	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26994	PYLLMGWTSNDHDSSLRYDV			20	SLAY-screened peptide P5344			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTACCTGCTTATGGGGTGGACCAGTAACGATCATGATAGCTCTTTGAGGTACGATGTTTAA	PYLLMGWTSNDHDSSLRYDV*	-1.098	0.031443	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26995	LIDWGGIPVLCCNICTNLLL			20	SLAY-screened peptide P5345			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCATCGATTGGGGTGGTATTCCCGTTTTGTGCTGCAACATTTGCACGAACTTGCTGCTTTAA	LIDWGGIPVLCCNICTNLLL*	-1.098	0.001706	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26996	NRSCICANNYTSFLSFPSCF			20	SLAY-screened peptide P5346			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCGTTCTTGTATCTGCGCTAATAATTACACCTCGTTTCTGTCCTTTCCGTCCTGTTTTTAA	NRSCICANNYTSFLSFPSCF*	-1.098	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26997	LIRNLCRMTHHYFSPK			16	SLAY-screened peptide P5347			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGATCAGGAATCTTTGCCGTATGACCCACCACTACTTTAGTCCTAAGTAGTATGGTGTCTAA	LIRNLCRMTHHYFSPK*YGV*	-1.098	0.001709	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26998	PLLHLSPNMSEPTWTWRTVH			20	SLAY-screened peptide P5348			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTTCTTCATCTCAGTCCGAATATGAGCGAGCCGACGTGGACGTGGAGGACGGTCCACTAA	PLLHLSPNMSEPTWTWRTVH*	-1.098	0.001558	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP26999	ADVVGDRPASLRQLALTRSE			20	SLAY-screened peptide P5349			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGATGTGGTTGGCGATCGTCCTGCCTCCCTGCGGCAGCTTGCGCTCACGCGCAGTGAGTAA	ADVVGDRPASLRQLALTRSE*	-1.098	0.000808	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27000	DPITMSCRSSSVSWTNNDFD			20	SLAY-screened peptide P5350			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCCGATTACTATGAGCTGTCGTTCGAGCTCTGTGTCCTGGACCAATAATGATTTTGATTAA	DPITMSCRSSSVSWTNNDFD*	-1.097	0.004217	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27001	APDVRATLPPKRAIYQAQP			19	SLAY-screened peptide P5351			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCTGACGTTCGCGCGACCCTCCCGCCCAAGCGTGCCATCTACCAGGCTCAGCCTTAGTAA	APDVRATLPPKRAIYQAQP**	-1.097	0.000278	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27002	HHSPAPGTWHELALFHVAGS			20	SLAY-screened peptide P5352			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCACTCCCCCGCCCCGGGCACTTGGCACGAGCTGGCCTTGTTTCACGTTGCCGGCTCCTAA	HHSPAPGTWHELALFHVAGS*	-1.097	0.000594	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27003	FTCSLLLRLMCLTFPRFFVQ			20	SLAY-screened peptide P5353			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCACGTGTAGCTTGCTTCTCCGCCTCATGTGCCTTACGTTTCCCAGGTTTTTTGTTCAGTAA	FTCSLLLRLMCLTFPRFFVQ*	-1.097	0.000223	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27004	ASAFDMSNLCSHVPDLNNLI			20	SLAY-screened peptide P5354			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTCCGCTTTTGACATGTCTAATTTGTGTTCCCATGTCCCTGACCTTAATAACCTGATTTAA	ASAFDMSNLCSHVPDLNNLI*	-1.096	0.001606	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27005	SDSTRSHSRNLHWVRTTIEV			20	SLAY-screened peptide P5355			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGACAGCACGCGGTCCCATTCCCGGAATCTCCACTGGGTCCGCACGACCATTGAGGTGTAA	SDSTRSHSRNLHWVRTTIEV*	-1.096	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27006	LIRSRSTCPRGLWYGYTGYS			20	SLAY-screened peptide P5356			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTATTCGCTCCCGTAGTACTTGTCCTAGGGGTCTTTGGTACGGTTACACTGGCTACAGTTAA	LIRSRSTCPRGLWYGYTGYS*	-1.096	0.000125	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27007	CPSFNSSARHYFMLVRSMTPN			21	SLAY-screened peptide P5357			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCCAGCTTTAATTCTTCCGCCAGGCATTATTTTATGTTGGTGAGATCAATGACTCCTAAC	CPSFNSSARHYFMLVRSMTPN	-1.095	0.008245	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27008	LAPSIAISPLVGLPRRRRLAN			21	SLAY-screened peptide P5358			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGCGCCGTCAATAGCAATTTCGCCATTGGTAGGACTTCCACGTAGAAGGCGTTTGGCTAAC	LAPSIAISPLVGLPRRRRLAN	-1.095	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27009	PCTYSTHLLYEPLLLTLLQ			19	SLAY-screened peptide P5359			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGCACGTACAGCACCCACCTTCTCTATGAGCCCCTCCTCCTCACCTTGTTGCAGTAGTAA	PCTYSTHLLYEPLLLTLLQ**	-1.095	0.043058	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27010	RVPGILKAPALCSRPPPVMH			20	SLAY-screened peptide P5360			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGTGCCTGGCATTCTGAAGGCCCCTGCGTTGTGCTCTCGTCCTCCTCCTGTTATGCACTAA	RVPGILKAPALCSRPPPVMH*	-1.095	0.002342	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27011	YGLDCCHCWQSGLYIAMNFS			20	SLAY-screened peptide P5361			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACGGTCTCGATTGCTGTCATTGCTGGCAGTCCGGTCTGTACATTGCTATGAATTTCAGTTAA	YGLDCCHCWQSGLYIAMNFS*	-1.095	0.0032	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27012	VFYSDWNYPSLCGKFYPTSH			20	SLAY-screened peptide P5362			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTTTTACTCTGATTGGAACTATCCCAGCCTGTGCGGGAAGTTCTACCCGACGAGCCACTAA	VFYSDWNYPSLCGKFYPTSH*	-1.095	0.049147	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27013	AQPTPLAPKHCFPILMAMYN			20	SLAY-screened peptide P5363			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCAGCCTACTCCTCTTGCCCCCAAGCACTGTTTTCCTATCCTCATGGCTATGTACAACTAA	AQPTPLAPKHCFPILMAMYN*	-1.095	0.002081	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27014	PHCPVTRIAPVREGIFTPFL			20	SLAY-screened peptide P5364			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCACTGTCCTGTTACTCGCATCGCCCCCGTGCGGGAGGGCATCTTCACCCCGTTTTTGTAA	PHCPVTRIAPVREGIFTPFL*	-1.095	0.000155	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27015	CTCYFPAPSPLGLYSSPVIL			20	SLAY-screened peptide P5365			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTACTTGCTACTTTCCCGCTCCGTCCCCTTTGGGGCTTTATTCGAGCCCTGTGATCCTCTAA	CTCYFPAPSPLGLYSSPVIL*	-1.095	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27016	PIPSPSWITYPVYVLAYDAH			20	SLAY-screened peptide P5366			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATTCCTTCTCCTTCCTGGATTACTTACCCTGTCTATGTCCTCGCCTATGACGCGCATTAA	PIPSPSWITYPVYVLAYDAH*	-1.094	0.012858	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27017	HMCNPSRPYNSQPTLSLIVL			20	SLAY-screened peptide P5367			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATATGTGTAACCCTAGCCGGCCTTACAATAGCCAGCCCACGCTGAGCCTCATCGTTCTCTAA	HMCNPSRPYNSQPTLSLIVL*	-1.094	0.013771	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27018	GACHELLNHPTPSNLLVL			18	SLAY-screened peptide P5368			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTGCTTGTCACGAGTTGCTGAACCATCCGACTCCGAGCAATTTGCTTGTTTTGTAGGTCTAA	GACHELLNHPTPSNLLVL*V*	-1.094	0.036846	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27019	TDTIKSAIANTHASNDPSAI			20	SLAY-screened peptide P5369			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGATACCATCAAGTCCGCTATTGCTAATACTCATGCCTCCAATGACCCGTCCGCCATTTAA	TDTIKSAIANTHASNDPSAI*	-1.094	0.028984	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27020	WPRAPPYTTRRCTTPAGDYP			20	SLAY-screened peptide P5370			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCCGCGCGCTCCGCCCTATACTACTCGTCGTTGTACCACGCCTGCGGGGGACTACCCGTAA	WPRAPPYTTRRCTTPAGDYP*	-1.094	0.001174	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27021	FDTKRVACCAMRWSSGHDPT			20	SLAY-screened peptide P5371			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGACACGAAGAGGGTTGCGTGTTGCGCTATGCGCTGGTCGAGCGGCCATGACCCTACGTAA	FDTKRVACCAMRWSSGHDPT*	-1.094	0.006548	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27022	APNVRGLKTCRLSSSTPYLP			20	SLAY-screened peptide P5372			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCCAACGTGCGCGGCCTTAAGACTTGTCGGCTCTCGTCGTCCACGCCTTATCTCCCTTAA	APNVRGLKTCRLSSSTPYLP*	-1.094	0.00311	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27023	RLDRLFCDPCATS			13	SLAY-screened peptide P5373			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCTTGACCGGCTCTTCTGCGACCCTTGCGCCACGAGTTAGTGGACTCCTAGTATTTGCTAA	RLDRLFCDPCATS*WTPSIC*	-1.093	0.023582	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27024	VAICSFIWLLFFKLRRNIDE			20	SLAY-screened peptide P5374			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGCCATTTGCTCCTTTATTTGGTTGCTTTTCTTTAAGCTGAGGAGGAATATCGATGAGTAA	VAICSFIWLLFFKLRRNIDE*	-1.093	0.048144	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27025	NDFLCVVRAPFWAHCNLWNC			20	SLAY-screened peptide P5375			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGACTTCCTCTGCGTCGTGAGGGCTCCCTTTTGGGCCCATTGCAATCTTTGGAATTGTTAA	NDFLCVVRAPFWAHCNLWNC*	-1.093	0.048182	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27026	LGVNNTRIFTISIP			14	SLAY-screened peptide P5376			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGGTGTCAATAACACGAGGATATTTACAATAAGTATTCCCTGAGCGGGGTGGTGTCGTAAC	LGVNNTRIFTISIP*AGWCRN	-1.093	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27027	ARPNTSPFMFHGWPCWHT			18	SLAY-screened peptide P5377			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCGGCCCAATACGTCTCCCTTTATGTTTCATGGTTGGCCCTGCTGGCACACCTAGACTTAA	ARPNTSPFMFHGWPCWHT*T*	-1.093	0.005291	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27028	CCPVHSSFKTCLSFTSPSYTI			21	SLAY-screened peptide P5378			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTTGCCCTGTTCACAGCTCGTTCAAGACCTGCCTTTCGTTTACGAGTCCTTCTTATACCATC	CCPVHSSFKTCLSFTSPSYTI	-1.093	0.009342	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27029	MSLIRRILRARLSYYSTRSS			20	SLAY-screened peptide P5379			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGAGCCTTATCCGCAGGATCTTGAGGGCTCGCCTCTCTTACTACTCTACGCGTAGCAGTTAA	MSLIRRILRARLSYYSTRSS*	-1.093	0.04677	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27030	VDPDTFHLDPLPLTLHYKDC			20	SLAY-screened peptide P5380			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGACCCGGACACCTTCCATCTCGACCCCCTTCCGCTGACTCTTCACTATAAGGATTGTTAA	VDPDTFHLDPLPLTLHYKDC*	-1.093	0.010717	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27031	HRRAGFTYGNASMRRLFYQA			20	SLAY-screened peptide P5381			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCGTCGTGCCGGCTTCACTTACGGGAACGCCAGCATGCGTCGGCTCTTTTACCAGGCGTAA	HRRAGFTYGNASMRRLFYQA*	-1.092	0.041031	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27032	HITLGAKLNTSPPYVQHMG			19	SLAY-screened peptide P5382			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACATCACGTTGGGCGCCAAGTTGAATACCAGCCCCCCCTACGTGCAGCATATGGGTTAGTAA	HITLGAKLNTSPPYVQHMG**	-1.092	0.03234	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27033	TKTDSLATAIKDDTHSRASP			20	SLAY-screened peptide P5383			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAAGACTGACTCCTTGGCTACCGCTATCAAGGACGATACTCATAGCCGCGCGTCTCCCTAA	TKTDSLATAIKDDTHSRASP*	-1.092	0.014856	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27034	PPAGNSDSDPLCRPNYNIRT			20	SLAY-screened peptide P5384			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCTGCGGGGAATTCGGATTCTGATCCTCTGTGTCGGCCGAACTATAACATTCGTACCTAA	PPAGNSDSDPLCRPNYNIRT*	-1.092	0.00273	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27035	CLFLCVVSTNTITLHDHSNP			20	SLAY-screened peptide P5385			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTCTTTCTCTGTGTGGTGAGCACGAACACTATCACCCTTCATGATCACTCCAATCCTTAA	CLFLCVVSTNTITLHDHSNP*	-1.092	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27036	RCNFCPRVLKLNPSVNNLSG			20	SLAY-screened peptide P5386			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGCAATTTTTGCCCCCGTGTTCTCAAGCTTAACCCCAGCGTTAACAACCTCTCGGGCTAA	RCNFCPRVLKLNPSVNNLSG*	-1.092	0.018121	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27037	DYPGLIVFALAPFYILSQYD			20	SLAY-screened peptide P5387			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTACCCCGGTTTGATCGTGTTTGCCCTGGCGCCGTTTTACATCCTTTCCCAGTATGACTAA	DYPGLIVFALAPFYILSQYD*	-1.092	0.006633	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27038	RTRVGIPPFLYRTPSLRIHS			20	SLAY-screened peptide P5388			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCACCCGCGTTGGTATTCCTCCGTTCCTGTACCGTACCCCTTCCCTTCGGATTCATTCCTAA	RTRVGIPPFLYRTPSLRIHS*	-1.092	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27039	FWHGLGLCNVYTI			13	SLAY-screened peptide P5389			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTTGGCACGGCCTCGGTTTGTGCAACGTTTATACTATCTAGAAGTGGCCTATTGACGATTAA	FWHGLGLCNVYTI*KWPIDD*	-1.091	0.014948	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27040	SVAPCTTCFPPTTDIHHNTI			20	SLAY-screened peptide P5390			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGTCGCCCCCTGCACGACTTGCTTTCCCCCCACTACTGATATTCATCATAACACCATCTAA	SVAPCTTCFPPTTDIHHNTI*	-1.091	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27041	GATTARYRPRISNWHTTYYF			20	SLAY-screened peptide P5391			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCGCGACGACTGCTCGTTACCGCCCGCGGATTTCTAACTGGCATACTACTTATTATTTCTAA	GATTARYRPRISNWHTTYYF*	-1.091	0.046399	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27042	ENNPFIDIRPTILDRLNLAN			20	SLAY-screened peptide P5392			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGAATAATCCTTTTATTGATATCCGCCCTACCATCCTTGACCGCCTCAATCTTGCGAACTAA	ENNPFIDIRPTILDRLNLAN*	-1.09	0.020731	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27043	PGYAWDTYYQHCSHSWKTYM			20	SLAY-screened peptide P5393			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGGTATGCGTGGGACACCTATTATCAGCATTGTTCCCATTCTTGGAAGACTTATATGTAA	PGYAWDTYYQHCSHSWKTYM*	-1.09	0.041707	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27044	HNPYSALSRVSNITYDYTLL			20	SLAY-screened peptide P5394			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAATCCTTATTCTGCGCTGAGCCGCGTTTCTAACATTACTTACGACTACACGTTGCTGTAA	HNPYSALSRVSNITYDYTLL*	-1.09	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27045	SRPLATDLPSIDPSTTFGSN			20	SLAY-screened peptide P5395			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCGGCCTCTTGCCACGGACTTGCCCTCGATCGACCCTTCTACCACTTTCGGTAGTAACTAA	SRPLATDLPSIDPSTTFGSN*	-1.09	0.021791	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27046	SVRLK			5	SLAY-screened peptide P5396			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGTGCGCTTGAAGTAGCTTACCGCCGACTCCGGTGCTCATACGGACACCGAGTGTATTTAA	SVRLK*LTADSGAHTDTECI*	-1.09	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27047	STTTPPFNPVHSVSDGVCVA			20	SLAY-screened peptide P5397			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTACCACCACTCCCCCCTTTAACCCCGTTCATAGCGTCAGCGACGGTGTGTGCGTTGCTTAA	STTTPPFNPVHSVSDGVCVA*	-1.09	0.002428	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27048	WVPPYNCIPHPDFFYIHSYH			20	SLAY-screened peptide P5398			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGGTCCCTCCGTACAACTGCATTCCCCACCCCGACTTTTTTTACATTCACAGCTATCACTAA	WVPPYNCIPHPDFFYIHSYH*	-1.09	0.007627	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27049	YHYPFPNVVTIVYVDLYCNV			20	SLAY-screened peptide P5399			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCACTATCCTTTCCCGAATGTGGTCACTATTGTGTACGTCGATCTTTATTGTAACGTTTAA	YHYPFPNVVTIVYVDLYCNV*	-1.09	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27050	LPTYVPLIPGVLNTLYYCII			20	SLAY-screened peptide P5400			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCCTACTTACGTCCCGCTTATTCCTGGTGTCCTCAATACCTTGTACTACTGCATCATTTAA	LPTYVPLIPGVLNTLYYCII*	-1.09	0.000855	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27051	GSSNDNLGRRRLVFNNSRSN			20	SLAY-screened peptide P5401			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTCCAGTAACGACAACCTCGGCCGTCGTCGCCTGGTGTTTAACAACTCCCGTAGCAACTAA	GSSNDNLGRRRLVFNNSRSN*	-1.089	0.036582	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27052	TPTVTGRCCRIMHIEGHSLT			20	SLAY-screened peptide P5402			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCGACCGTCACTGGGCGCTGTTGTCGGATTATGCATATCGAGGGTCACTCTCTCACGTAA	TPTVTGRCCRIMHIEGHSLT*	-1.089	0.000713	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27053	VSSGVGNPNFSDAAGVPTYT			20	SLAY-screened peptide P5403			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTAGCTCGGGCGTTGGTAACCCCAATTTTAGCGATGCGGCTGGCGTTCCTACGTACACGTAA	VSSGVGNPNFSDAAGVPTYT*	-1.089	0.011956	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27054	HNTYCNTPYSYHSL			14	SLAY-screened peptide P5404			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAATACGTATTGCAACACTCCCTATAGCTACCACAGTCTTTAGGTCGACCGTCATCTTTAA	HNTYCNTPYSYHSL*VDRHL*	-1.088	0.029526	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27055	PGHSGSPMQYNTL			13	SLAY-screened peptide P5405			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGGTCACAGTGGGTCCCCCATGCAGTACAATACGCTGTAGCTGTTGCCTAGGATGCATTAA	PGHSGSPMQYNTL*LLPRMH*	-1.088	0.006138	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27056	RLDPHMENIFWSPHHMFHHN			20	SLAY-screened peptide P5406			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTTGGATCCTCATATGGAGAACATCTTCTGGTCCCCGCACCACATGTTTCATCATAATTAA	RLDPHMENIFWSPHHMFHHN*	-1.088	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27057	EATPFYRSVPTLYARCRAPP			20	SLAY-screened peptide P5407			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGCTACTCCTTTCTATCGCAGCGTCCCTACCCTGTACGCTCGGTGCCGTGCGCCTCCCTAA	EATPFYRSVPTLYARCRAPP*	-1.088	0.007218	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27058	WMNYNYTGYASWYC			14	SLAY-screened peptide P5408			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGATGAATTATAATTACACGGGCTACGCCAGCTGGTATTGCTAGTGTGCTCACAAGATTTAA	WMNYNYTGYASWYC*CAHKI*	-1.088	0.011703	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27059	FVESDDRIIYTLAPMTSIVV			20	SLAY-screened peptide P5409			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGTGGAGTCCGACGACCGCATTATCTACACCCTTGCCCCCATGACTTCCATCGTTGTCTAA	FVESDDRIIYTLAPMTSIVV*	-1.087	0.002474	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27060	LTSPPNSPYPPLFWRPCLIF			20	SLAY-screened peptide P5410			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGACTTCGCCCCCGAACAGTCCCTATCCGCCTCTCTTCTGGCGCCCGTGTTTGATTTTCTAA	LTSPPNSPYPPLFWRPCLIF*	-1.087	0.00015	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27061	SCTHPAACTIGGYPWN			16	SLAY-screened peptide P5411			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTGCACTCACCCGGCCGCCTGTACCATTGGTGGTTACCCTTGGAACTAGTGGCACGCCTAA	SCTHPAACTIGGYPWN*WHA*	-1.086	0.000388	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27062	RDYPKYRFLNEYHILDSLTC			20	SLAY-screened peptide P5412			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGATTATCCTAAGTATCGGTTCTTGAATGAGTACCATATTCTTGACTCGTTGACTTGTTAA	RDYPKYRFLNEYHILDSLTC*	-1.086	0.015544	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27063	VLSFLSKLLCPSVHRTTNNL			20	SLAY-screened peptide P5413			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCTTAGCTTCCTTTCTAAGCTGCTGTGCCCCTCTGTGCATCGCACTACTAACAACCTTTAA	VLSFLSKLLCPSVHRTTNNL*	-1.085	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27064	GCGCATMSCLSLLVDTPLWF			20	SLAY-screened peptide P5414			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTGCGGCTGTGCCACGATGTCTTGCCTCTCTCTGTTGGTTGATACTCCGCTCTGGTTTTAA	GCGCATMSCLSLLVDTPLWF*	-1.085	0.006726	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27065	CAGLLWGFRSGNSGTYWYM			19	SLAY-screened peptide P5415			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCCGGCCTGCTGTGGGGTTTTCGGTCCGGGAATTCGGGGACCTATTGGTATATGTAGTAA	CAGLLWGFRSGNSGTYWYM**	-1.085	0.013194	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27066	HHSNESRG			8	SLAY-screened peptide P5416			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCACAGTAACGAGAGTCGCGGTTAGATGATTATTAAGCCCGACAGCAGGGATCGTATGTAA	HHSNESRG*MIIKPDSRDRM*	-1.085	0.000176	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27067	TSCYHMGLL			9	SLAY-screened peptide P5417			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTCCTGTTACCACATGGGTCTCCTTTAACGATGCTGGCGTCCCGGAGGTCTATCATACGTA	TSCYHMGLL*RCWRPGGLSYV	-1.085	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27068	PENTYNHPEYRTVIYSYI			18	SLAY-screened peptide P5418			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGAGAACACCTATAACCACCCTGAGTATCGTACCGTCATCTACAGCTATATCTAGAACTAA	PENTYNHPEYRTVIYSYI*N*	-1.085	0.012091	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27069	NPVPFYSPHLTDPHWILITF			20	SLAY-screened peptide P5419			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATCCCGTGCCCTTTTATTCTCCCCACCTCACCGATCCGCATTGGATTTTGATCACGTTTTAA	NPVPFYSPHLTDPHWILITF*	-1.084	3.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27070	MYFNPSCYTIV			11	SLAY-screened peptide P5420			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTACTTCAACCCTAGCTGTTATACTATTGTGTAGCTCATTTTTAACATTAAGTCTCAGTAA	MYFNPSCYTIV*LIFNIKSQ*	-1.084	0.00283	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27071	CHYDRNRTATTRASYQVTPK			20	SLAY-screened peptide P5421			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCACTACGACCGTAATCGCACTGCCACCACGAGGGCGTCCTATCAGGTTACTCCCAAGTAA	CHYDRNRTATTRASYQVTPK*	-1.084	0.007894	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27072	NLNTHYLTWYLTTNTQH			17	SLAY-screened peptide P5422			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTTGAACACCCATTACCTCACCTGGTACCTGACGACTAATACGCAGCATTAGATCCTCTAA	NLNTHYLTWYLTTNTQH*IL*	-1.084	0.000861	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27073	PQPKSGPSARSSTMRAKRRR			20	SLAY-screened peptide P5423			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCAGCCGAAGTCCGGTCCTAGCGCTCGCTCCTCCACGATGCGCGCTAAGCGTCGTAGGTAA	PQPKSGPSARSSTMRAKRRR*	-1.083	2.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27074	NTSQVCSPRRASHSVVRDYVT			21	SLAY-screened peptide P5424			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACACGTCACAAGTGTGCTCACCGCGGCGCGCCTCACATTCCGTCGTACGCGACTACGTAACT	NTSQVCSPRRASHSVVRDYVT	-1.083	0.006851	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27075	QHPFVGPSIHTALWSVFIDP			20	SLAY-screened peptide P5425			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCACCCGTTCGTCGGGCCTAGTATTCACACCGCGCTCTGGAGCGTTTTTATCGACCCTTAA	QHPFVGPSIHTALWSVFIDP*	-1.083	0.022784	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27076	VLRRWLVRGVSCLCACPPPSN			21	SLAY-screened peptide P5426			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCTACGCCGATGGCTTGTAAGGGGAGTGTCTTGCCTGTGCGCTTGCCCACCCCCGTCTAAC	VLRRWLVRGVSCLCACPPPSN	-1.082	0.011328	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27077	PCDFLAVHATNKTSHHIFDY			20	SLAY-screened peptide P5427			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGTGATTTTCTTGCCGTCCACGCTACCAATAAGACCTCTCACCACATTTTTGATTACTAA	PCDFLAVHATNKTSHHIFDY*	-1.082	0.0313	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27078	RNDFTFAPTVCPAYSFVVLP			20	SLAY-screened peptide P5428			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAACGACTTTACTTTTGCTCCGACTGTGTGCCCCGCCTATTCGTTTGTTGTCTTGCCGTAA	RNDFTFAPTVCPAYSFVVLP*	-1.082	0.000689	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27079	HCLELSPNYSLIHFL			15	SLAY-screened peptide P5429			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGCCTTGAGCTCAGTCCCAATTATTCCCTTATTCATTTTCTTTAGAACTGGCGGACTTAA	HCLELSPNYSLIHFL*NWRT*	-1.082	0.043407	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27080	HCNNYFSHSYHYCTAFLYYV			20	SLAY-screened peptide P5430			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTGTAACAATTACTTTTCTCATTCTTATCACTACTGCACTGCCTTCTTGTACTACGTGTAA	HCNNYFSHSYHYCTAFLYYV*	-1.082	0.009018	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27081	TTPWPYDFRPCMLHYTHEAI			20	SLAY-screened peptide P5431			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTACCCCCTGGCCTTACGATTTCCGGCCTTGTATGCTTCACTACACGCACGAGGCTATTTAA	TTPWPYDFRPCMLHYTHEAI*	-1.082	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27082	QWTCNY			6	SLAY-screened peptide P5432			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTGGACTTGTAATTACTAGGACATTCGTACTGTGAGCAGCACTGGTCTCAAGAGTCCGTAA	QWTCNY*DIRTVSSTGLKSP*	-1.082	0.042206	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27083	KTNRYLPTMFNFRCKDDAVV			20	SLAY-screened peptide P5433			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGACGAACCGGTATCTCCCTACGATGTTCAACTTCCGGTGTAAGGATGATGCGGTCGTCTAA	KTNRYLPTMFNFRCKDDAVV*	-1.082	0.040967	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27084	FSFFWVSNNFYYTHPNTAFG			20	SLAY-screened peptide P5434			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTAGCTTTTTTTGGGTGAGCAACAATTTCTATTACACTCACCCTAATACTGCTTTTGGGTAA	FSFFWVSNNFYYTHPNTAFG*	-1.082	0.001943	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27085	SMKPWFAEEPLRCIHYIT			18	SLAY-screened peptide P5435			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGATGAAGCCTTGGTTTGCCGAGGAGCCCCTCCGCTGCATTCACTATATTACCTAGACTTAA	SMKPWFAEEPLRCIHYIT*T*	-1.081	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27086	QNTGTCPSARMFSPISPFNA			20	SLAY-screened peptide P5436			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGAATACTGGCACGTGCCCCAGTGCGCGCATGTTCAGTCCGATTTCCCCCTTTAATGCGTAA	QNTGTCPSARMFSPISPFNA*	-1.081	0.015422	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27087	VPSSATFSRATRPPVTTGSR			20	SLAY-screened peptide P5437			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCCCTCGAGTGCTACTTTCTCTAGGGCGACTCGCCCCCCGGTTACGACGGGCAGTCGCTAA	VPSSATFSRATRPPVTTGSR*	-1.081	0.011233	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27088	DSSNRVYSSYWRPYSMTLSI			20	SLAY-screened peptide P5438			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATAGTTCCAATCGTGTCTATTCGTCCTACTGGCGTCCCTACTCTATGACCCTGAGCATCTAA	DSSNRVYSSYWRPYSMTLSI*	-1.081	0.003315	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27089	HSLDSTNGCTH			11	SLAY-screened peptide P5439			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTCCCTTGATAGCACCAACGGTTGTACGCACTAGACTTATATTATCGACTTCTGTCTCTAA	HSLDSTNGCTH*TYIIDFCL*	-1.081	0.017778	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27090	TFSLKVFPAVLISTHINPRF			20	SLAY-screened peptide P5440			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTTCTCCCTCAAGGTTTTCCCGGCGGTTCTCATTTCGACGCATATCAACCCCCGGTTCTAA	TFSLKVFPAVLISTHINPRF*	-1.08	0.012251	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27091	SPTSVAYLPPVLYVRHSRSW			20	SLAY-screened peptide P5441			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCCCACCTCCGTTGCCTACCTTCCTCCTGTTCTGTACGTTCGTCACTCTCGTAGTTGGTAA	SPTSVAYLPPVLYVRHSRSW*	-1.08	0.039467	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27092	YCWNPLFYTVGCATPSWLFVN			21	SLAY-screened peptide P5442			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTGCTGGAACCCGCTCTTCTATACTGTCGGTTGTGCAACGCCCAGTTGGCTATTCGTTAAC	YCWNPLFYTVGCATPSWLFVN	-1.08	0.001087	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27093	GFRCTDTSQGGSLTTSFLLCN			21	SLAY-screened peptide P5443			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTTCCGTTGCACGGACACCTCGCAAGGAGGGTCCCTAACAACTTCCTTCCTCCTATGTAAC	GFRCTDTSQGGSLTTSFLLCN	-1.08	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27094	CTCCYMPSRHFKAFTTIRSV			20	SLAY-screened peptide P5444			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTACTTGCTGTTACATGCCCAGTAGGCACTTTAAGGCCTTCACCACTATTCGGAGTGTTTAA	CTCCYMPSRHFKAFTTIRSV*	-1.08	0.001935	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27095	PSLWGHWYWQPMILYSVA			18	SLAY-screened peptide P5445			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGAGCCTGTGGGGTCATTGGTACTGGCAGCCTATGATCTTGTACAGTGTTGCTTAGCATTAA	PSLWGHWYWQPMILYSVA*H*	-1.08	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27096	HPVLPADSDPERSQITCYIT			20	SLAY-screened peptide P5446			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCCTGTCCTTCCGGCCGATAGCGACCCTGAGCGGTCCCAGATCACCTGTTACATCACCTAA	HPVLPADSDPERSQITCYIT*	-1.079	0.027682	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27097	SLRASLHGNCLPILSSDRNA			20	SLAY-screened peptide P5447			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTTGCGTGCCTCGCTGCATGGGAACTGCCTTCCTATTCTCTCTAGTGACCGCAACGCGTAA	SLRASLHGNCLPILSSDRNA*	-1.079	0.006624	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27098	HNTCVAWLSYTRLE			14	SLAY-screened peptide P5448			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAATACCTGCGTCGCCTGGCTTTCCTATACTCGTCTTGAGTAGCTTACGGCGGTGGTTAAC	HNTCVAWLSYTRLE*LTAVVN	-1.079	0.012067	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27099	DLFRVSHY			8	SLAY-screened peptide P5449			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCTTTTTCGGGTTTCCCATTATTAGCACCCTTATACTGCGCCTCCGCGCCTTCGCTTTTAA	DLFRVSHY*HPYTAPPRLRF*	-1.079	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27100	IARNPARTLSRLRLSGPLLN			20	SLAY-screened peptide P5450			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGCCCGCAATCCCGCCCGCACGTTGTCTCGGTTACGGCTTAGCGGTCCTCTACTTAACTGA	IARNPARTLSRLRLSGPLLN*	-1.079	0.000389	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27101	PRSWFCNYFHPRLYRMRRRR			20	SLAY-screened peptide P5451			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGCTCGTGGTTCTGCAACTACTTTCATCCGAGGCTTTATCGTATGCGTAGGCGGCGTTAA	PRSWFCNYFHPRLYRMRRRR*	-1.079	0.000625	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27102	CMHLNPYNYFYFVRYDKNGR			20	SLAY-screened peptide P5452			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCATGCACCTTAACCCCTACAATTACTTTTATTTCGTTAGGTACGATAAGAATGGTCGTTAA	CMHLNPYNYFYFVRYDKNGR*	-1.079	0.001449	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27103	TLCPTVWDVI			10	SLAY-screened peptide P5453			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCTCTGTCCTACGGTTTGGGACGTTATCTAGTCTAAGTGGAATACTCGGGACATGGGCTAA	TLCPTVWDVI*SKWNTRDMG*	-1.078	2.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27104	HNPCFRALVFVLCVLMTPVK			20	SLAY-screened peptide P5454			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAACCCTTGTTTCCGGGCCCTTGTTTTTGTTCTTTGCGTCCTTATGACCCCCGTCAAGTAA	HNPCFRALVFVLCVLMTPVK*	-1.078	0.000673	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27105	SYIPFSCPWLYSDLSTSQFL			20	SLAY-screened peptide P5455			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTACATCCCGTTTAGCTGTCCTTGGCTCTACAGCGACCTGAGCACTTCCCAGTTTCTCTAA	SYIPFSCPWLYSDLSTSQFL*	-1.078	0.040435	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27106	SANNLIFHCPYQFLPFMPLL			20	SLAY-screened peptide P5456			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGCTAACAACCTCATTTTCCATTGTCCCTACCAGTTTCTGCCCTTCATGCCGCTCTTGTAA	SANNLIFHCPYQFLPFMPLL*	-1.077	0.039996	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27107	SGSFAPSCYSPHYNARKVHN			20	SLAY-screened peptide P5457			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGGTTCCTTTGCCCCGTCCTGTTACAGTCCCCATTACAACGCTCGGAAGGTTCATAACTAA	SGSFAPSCYSPHYNARKVHN*	-1.077	0.000406	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27108	AHFENCGSGWVYSPSNLYLI			20	SLAY-screened peptide P5458			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCATTTTGAGAATTGCGGTTCGGGTTGGGTTTACTCTCCTTCCAACCTCTACCTTATCTAA	AHFENCGSGWVYSPSNLYLI*	-1.077	0.021338	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27109	AISDYPNFFWSCYIL			15	SLAY-screened peptide P5459			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCATTTCCGATTACCCTAACTTTTTTTGGAGCTGTTACATTCTCTAGTTTTTTCGCTATTAA	AISDYPNFFWSCYIL*FFRY*	-1.077	1.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27110	GRGTVATCYNPTGYEYL			17	SLAY-screened peptide P5460			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCGCGGTACCGTCGCGACTTGTTATAACCCCACTGGGTATGAGTACCTGTAACTGAGTAAG	GRGTVATCYNPTGYEYL*LSK	-1.077	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27111	HSYSCQDPGSEFTKIHQWQA			20	SLAY-screened peptide P5461			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTCGTATTCCTGTCAGGACCCGGGTTCCGAGTTCACTAAGATCCACCAGTGGCAGGCCTAA	HSYSCQDPGSEFTKIHQWQA*	-1.077	0.001952	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27112	DPRVANRGRQRLMEHH			16	SLAY-screened peptide P5462			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCCGAGGGTCGCGAATCGCGGCCGCCAGCGCCTGATGGAGCATCACTAGGACACTAAGTAA	DPRVANRGRQRLMEHH*DTK*	-1.076	0.010508	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27113	CRAQGAPVVICRGSSCNIMT			20	SLAY-screened peptide P5463			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGGGCCCAGGGGGCTCCGGTCGTTATCTGCCGTGGCTCCAGCTGCAACATTATGACCTAA	CRAQGAPVVICRGSSCNIMT*	-1.076	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27114	PRADAFTFAGSGPKVVTIIS			20	SLAY-screened peptide P5464			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGTGCCGACGCGTTTACCTTTGCCGGGTCTGGTCCTAAGGTTGTTACGATTATTTCTTAA	PRADAFTFAGSGPKVVTIIS*	-1.076	0.009341	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27115	LPDDFYHNVLICVKRDVNYT			20	SLAY-screened peptide P5465			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCCTGATGATTTCTACCATAACGTCCTGATCTGCGTGAAGCGTGATGTTAACTACACTTAA	LPDDFYHNVLICVKRDVNYT*	-1.076	0.026804	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27116	PADGVDKAYWCGPVFCVWYL			20	SLAY-screened peptide P5466			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCTGACGGCGTCGACAAAGCTTACTGGTGTGGGCCCGTCTTTTGTGTGTGGTATTTGTAA	PADGVDKAYWCGPVFCVWYL*	-1.076	0.001026	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27117	QFGRVAIIISRGRGNPNIVG			20	SLAY-screened peptide P5467			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTTTGGTAGGGTTGCTATTATCATCTCTCGCGGCCGTGGCAATCCCAACATTGTGGGCTAA	QFGRVAIIISRGRGNPNIVG*	-1.075	0.000158	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27118	DDANSRHEPPHYYCAPHILL			20	SLAY-screened peptide P5468			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACGATGCCAATTCGCGCCATGAGCCTCCTCACTACTATTGTGCGCCTCACATTCTCCTTTAA	DDANSRHEPPHYYCAPHILL*	-1.075	0.000499	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27119	YLDNSNDKVSLSHSTNISNN			20	SLAY-screened peptide P5469			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCTCGATAATTCTAATGACAAGGTTTCGCTTTCGCATTCCACCAACATCAGTAACAACTAA	YLDNSNDKVSLSHSTNISNN*	-1.075	0.001001	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27120	ILRYFVNWLKAHTVRPVPYV			20	SLAY-screened peptide P5470			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCTTCGTTACTTCGTTAACTGGTTGAAGGCCCACACTGTTCGGCCCGTGCCGTATGTCTAA	ILRYFVNWLKAHTVRPVPYV*	-1.075	0.005062	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27121	NSPVAKFSPIYPSMLLA			17	SLAY-screened peptide P5471			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTCCCCCGTTGCTAAGTTCAGTCCTATCTACCCTAGCATGCTGCTTGCCTAGATGTGTTAA	NSPVAKFSPIYPSMLLA*MC*	-1.074	2.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27122	SRCLEMASGIIPFAFMNHNG			20	SLAY-screened peptide P5472			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCGTTGTCTGGAGATGGCCTCCGGCATCATTCCTTTTGCTTTTATGAATCACAACGGTTAA	SRCLEMASGIIPFAFMNHNG*	-1.074	0.043974	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27123	WFPCSWGNNHPYNYFRRSDL			20	SLAY-screened peptide P5473			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTTCCCGTGTTCCTGGGGTAATAATCACCCTTACAATTATTTTCGTCGTTCGGACCTGTAA	WFPCSWGNNHPYNYFRRSDL*	-1.074	0.023274	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27124	PPIDCKQLLPMWAYKLIKSN			20	SLAY-screened peptide P5474			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCCATTGACTGCAAGCAGCTCCTCCCTATGTGGGCCTATAAGTTGATCAAGAGCAATTAA	PPIDCKQLLPMWAYKLIKSN*	-1.074	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27125	LSAPSPSYEPLTFTPTCYPD			20	SLAY-screened peptide P5475			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCGGCCCCGTCGCCGTCGTATGAGCCCTTGACTTTTACTCCTACTTGTTACCCTGATTAA	LSAPSPSYEPLTFTPTCYPD*	-1.074	0.001019	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27126	SSMTPLWHAPLWCHLSQFIV			20	SLAY-screened peptide P5476			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTCTATGACCCCTCTCTGGCATGCCCCCTTGTGGTGCCACCTGAGCCAGTTTATTGTTTAA	SSMTPLWHAPLWCHLSQFIV*	-1.073	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27127	FRALRHLLNSCFNTDHQLRP			20	SLAY-screened peptide P5477			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCGGGCCCTTCGTCACTTGCTTAACAGCTGTTTCAATACTGACCACCAGCTTAGGCCCTAA	FRALRHLLNSCFNTDHQLRP*	-1.073	0.005856	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27128	AGFIHPYFPRLIVATFNDNY			20	SLAY-screened peptide P5478			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGGCTTTATCCATCCCTATTTTCCCAGGCTTATTGTCGCTACCTTTAACGACAATTACTAA	AGFIHPYFPRLIVATFNDNY*	-1.073	0.042935	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27129	PMRIKCIGDSTALFEKNKSC			20	SLAY-screened peptide P5479			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATGCGTATTAAGTGCATCGGGGATAGCACCGCGCTTTTTGAGAAGAATAAGTCTTGCTAA	PMRIKCIGDSTALFEKNKSC*	-1.073	0.000112	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27130	PAILIYRPWCLYVDLPWP			18	SLAY-screened peptide P5480			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGCTATCCTGATCTACCGCCCCTGGTGTCTGTACGTTGACTTGCCGTGGCCCTAGCTGTAA	PAILIYRPWCLYVDLPWP*L*	-1.073	0.007841	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27131	LHTYLLNSTRCWRQVSFFYA			20	SLAY-screened peptide P5481			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCACACTTATCTCCTCAATTCTACTCGGTGCTGGCGTCAGGTGTCTTTTTTCTATGCCTAA	LHTYLLNSTRCWRQVSFFYA*	-1.073	0.01233	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27132	TFTNKISFCTIITIRIVFTFN			21	SLAY-screened peptide P5482			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTTCACCAATAAGATATCTTTCTGCACTATCATCACGATACGCATAGTTTTCACCTTTAAC	TFTNKISFCTIITIRIVFTFN	-1.072	0.00523	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27133	PLARPIHFDTFQCYGSYSEP			20	SLAY-screened peptide P5483			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTCGCTCGGCCTATTCATTTTGATACGTTTCAGTGTTATGGCTCCTACTCTGAGCCCTAA	PLARPIHFDTFQCYGSYSEP*	-1.072	0.013613	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27134	CIVDMATSYSNHFRTWTM			18	SLAY-screened peptide P5484			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTATCGTTGACATGGCCACTAGCTACTCCAATCACTTTCGCACCTGGACTATGTAGACTTAA	CIVDMATSYSNHFRTWTM*T*	-1.072	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27135	PHLRTCNDLPILKPWRILSA			20	SLAY-screened peptide P5485			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCACCTTCGTACCTGCAATGATCTTCCCATCTTGAAGCCCTGGCGCATCCTTAGTGCCTAA	PHLRTCNDLPILKPWRILSA*	-1.072	0.002376	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27136	GYCLLPTFSLACLTLMLPMI			20	SLAY-screened peptide P5486			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTACTGCTTGCTTCCTACCTTTTCTCTGGCGTGTTTGACGCTGATGCTTCCTATGATCTAA	GYCLLPTFSLACLTLMLPMI*	-1.072	0.004808	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27137	HCVRLDDPGIPLWVCTAFPG			20	SLAY-screened peptide P5487			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTGCGTCAGGCTTGATGACCCCGGTATCCCCCTTTGGGTCTGCACCGCGTTCCCGGGTTAA	HCVRLDDPGIPLWVCTAFPG*	-1.072	0.001182	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27138	INPSISYDVISLLLLLNFFL			20	SLAY-screened peptide P5488			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAATCCCAGCATTAGTTACGATGTCATTAGTCTGCTCCTCCTGCTTAACTTTTTTCTTTAA	INPSISYDVISLLLLLNFFL*	-1.071	3.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27139	GHSYDSWRYGILPIQTLFFQ			20	SLAY-screened peptide P5489			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCCATAGTTATGATTCTTGGCGGTACGGTATTCTGCCCATCCAGACCCTGTTTTTTCAGTAA	GHSYDSWRYGILPIQTLFFQ*	-1.071	0.018559	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27140	SAHDSCPRNFYLSHMAPHRF			20	SLAY-screened peptide P5490			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGCTCACGATAGCTGCCCGCGCAACTTCTACTTGAGTCACATGGCTCCTCATCGCTTTTAA	SAHDSCPRNFYLSHMAPHRF*	-1.071	0.001688	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27141	SPWRCIRSCLHDIFFSHSHS			20	SLAY-screened peptide P5491			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCCTTGGAGGTGCATCAGGTCCTGCCTCCACGATATCTTTTTTTCCCATTCGCACTCCTAA	SPWRCIRSCLHDIFFSHSHS*	-1.07	0.005207	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27142	ANVPYSPIMFPGSSLRSSNF			20	SLAY-screened peptide P5492			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGAACGTCCCCTATTCGCCTATTATGTTTCCTGGCTCTTCTCTTCGTTCCTCCAATTTTTAA	ANVPYSPIMFPGSSLRSSNF*	-1.07	0.003726	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27143	QHTYQMTDRHNRGPPVILSH			20	SLAY-screened peptide P5493			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCACACTTACCAGATGACCGATCGGCATAACCGCGGCCCTCCGGTTATCCTGAGCCACTAA	QHTYQMTDRHNRGPPVILSH*	-1.07	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27144	HLPCYPYFFRTRSPQMYTPT			20	SLAY-screened peptide P5494			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCTTCCCTGTTACCCCTATTTCTTCCGGACGCGCAGCCCCCAGATGTATACTCCCACTTAA	HLPCYPYFFRTRSPQMYTPT*	-1.07	0.030165	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27145	ASPEDNVNASKSSRLIIIIN			20	SLAY-screened peptide P5495			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCAGTCCTGAGGACAATGTGAACGCTTCCAAGAGCTCTCGACTCATAATCATAATTAACTGA	ASPEDNVNASKSSRLIIIIN*	-1.07	0.037519	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27146	GFHRRSDSFTNMTSRLASSFN			21	SLAY-screened peptide P5496			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCTTCCATCGTCGTTCCGACAGTTTTACTAACATGACTAGTCGTCTGGCTTCCTCGTTTAAC	GFHRRSDSFTNMTSRLASSFN	-1.07	0.012038	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27147	NRTGNELRNFSLCNHHDWVL			20	SLAY-screened peptide P5497			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCGTACGGGTAACGAGCTGCGCAACTTCTCGCTCTGTAACCATCACGATTGGGTCTTGTAA	NRTGNELRNFSLCNHHDWVL*	-1.069	0.005206	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27148	CDLSLSSWTIAAN			13	SLAY-screened peptide P5498			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGATTTGTCCTTGTCCAGTTGGACGATTGCGGCTAACTAGTCCTACTTGATTAATACGTAA	CDLSLSSWTIAAN*SYLINT*	-1.069	0.003111	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27149	WQPPCWSFQSWNPVTSPYLP			20	SLAY-screened peptide P5499			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCAGCCGCCCTGTTGGTCTTTCCAGAGTTGGAACCCCGTTACGTCCCCCTATCTTCCTTAA	WQPPCWSFQSWNPVTSPYLP*	-1.069	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27150	SRRLPHWHDCSTFYLNDHLY			20	SLAY-screened peptide P5500			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCGTCGGTTGCCCCATTGGCACGACTGCAGTACTTTTTATCTCAACGATCATTTGTACTAA	SRRLPHWHDCSTFYLNDHLY*	-1.069	0.000361	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27151	KYVYTTNTTH			10	SLAY-screened peptide P5501			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGTACGTTTACACGACCAATACCACCCATTAGCATCACAGCACGCTTTATAGGTACCCGTAA	KYVYTTNTTH*HHSTLYRYP*	-1.069	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27152	PTTYCTLLFT			10	SLAY-screened peptide P5502			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACTACCTATTGCACGCTGCTCTTTACGTAGAGTTACCGGGATCTTAGCGCTAACTTGTAA	PTTYCTLLFT*SYRDLSANL*	-1.069	0.005397	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27153	PFSPSPTRLYAFHGFSRLTC			20	SLAY-screened peptide P5503			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTTTCGCCGTCGCCTACCCGGCTCTACGCTTTTCATGGCTTCTCTCGTCTCACCTGCTAA	PFSPSPTRLYAFHGFSRLTC*	-1.069	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27154	YFCTPIDGESKRISYHYLFH			20	SLAY-screened peptide P5504			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTTCTGCACTCCCATTGATGGGGAGTCTAAGAGGATTTCCTACCATTATTTGTTTCATTAA	YFCTPIDGESKRISYHYLFH*	-1.068	0.028752	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27155	AYPLHYDVHYNYPCSYTKFG			20	SLAY-screened peptide P5505			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTATCCTCTCCACTACGACGTTCATTACAACTATCCGTGCAGTTATACTAAGTTTGGGTAA	AYPLHYDVHYNYPCSYTKFG*	-1.068	0.0027	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27156	VTPFPVPPHLGPWTVPMRVF			20	SLAY-screened peptide P5506			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCACCCCGTTCCCGGTCCCTCCTCACCTCGGTCCTTGGACTGTGCCGATGCGTGTCTTTTAA	VTPFPVPPHLGPWTVPMRVF*	-1.068	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27157	CHGPPSMPPWFVLTYQRAND			20	SLAY-screened peptide P5507			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCATGGGCCCCCTTCCATGCCGCCTTGGTTTGTTCTCACCTATCAGCGGGCGAATGATTAA	CHGPPSMPPWFVLTYQRAND*	-1.068	0.002071	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27158	FTASLSLLSLNHSSPYLHVH			20	SLAY-screened peptide P5508			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCACGGCGTCGCTCTCCCTCCTCTCTCTGAATCATAGCTCGCCCTATCTTCACGTCCATTAA	FTASLSLLSLNHSSPYLHVH*	-1.068	0.012631	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27159	VSVLHFSWSRLTTLSITLIN			20	SLAY-screened peptide P5509			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCAGCGTCCTGCATTTCTCCTGGAGTCGCCTTACGACCCTCTCGATCACGCTGATCAATTAA	VSVLHFSWSRLTTLSITLIN*	-1.068	0.026879	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27160	RPSTSHPWLSTGLLWRTYLL			20	SLAY-screened peptide P5510			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGCCCTCCACGTCCCATCCCTGGCTCTCCACGGGTTTGTTGTGGCGTACGTATCTGCTGTAA	RPSTSHPWLSTGLLWRTYLL*	-1.067	0.015774	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27161	FTPCRPLCDLDIPARNRCPW			20	SLAY-screened peptide P5511			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCACCCCCTGCCGCCCGCTCTGTGATCTCGACATTCCGGCCCGCAACCGGTGTCCTTGGTAA	FTPCRPLCDLDIPARNRCPW*	-1.067	0.029556	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27162	YRSGHGVTAYTLGLSQVHSS			20	SLAY-screened peptide P5512			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCGTAGCGGTCACGGCGTGACCGCGTACACCTTGGGGCTTTCTCAGGTCCATAGTTCTTAA	YRSGHGVTAYTLGLSQVHSS*	-1.067	0.011261	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27163	PDPRLFFRKLPFDYSWTLLA			20	SLAY-screened peptide P5513			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGATCCTCGTCTGTTTTTCCGGAAGCTGCCGTTTGATTATTCTTGGACGCTGCTGGCTTAA	PDPRLFFRKLPFDYSWTLLA*	-1.067	0.001298	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27164	CTYHLSTTSRSIPMMLTSIA			20	SLAY-screened peptide P5514			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTACTTACCACTTGTCTACCACGAGTCGGTCCATCCCTATGATGCTCACGTCCATCGCCTAA	CTYHLSTTSRSIPMMLTSIA*	-1.067	0.040637	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27165	CKLFPSLL			8	SLAY-screened peptide P5515			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAAGCTTTTTCCTAGTCTGCTCTAGTCTCGTCAGCACCGTCGCCGTGTGAAGCCCCTGTAA	CKLFPSLL*SRQHRRRVKPL*	-1.067	0.003349	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27166	GTSTMDPANHLPHSINPLYP			20	SLAY-screened peptide P5516			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCACCTCGACGATGGACCCCGCTAACCATCTCCCTCACTCTATCAATCCTCTGTATCCCTAA	GTSTMDPANHLPHSINPLYP*	-1.067	0.008361	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27167	FSPGCLFLACITDCNSGLSG			20	SLAY-screened peptide P5517			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCTCCCCCGGGTGCCTTTTTCTGGCCTGCATTACGGATTGTAACAGTGGTTTGTCCGGTTAA	FSPGCLFLACITDCNSGLSG*	-1.066	8.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27168	NDAEKGSPVFHCPVYI			16	SLAY-screened peptide P5518			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATGATGCCGAGAAGGGTAGCCCCGTTTTTCATTGCCCTGTTTACATCTAGCACGCTCCCTAA	NDAEKGSPVFHCPVYI*HAP*	-1.066	5.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27169	ATLYYQPCYFPASSYFTEHR			20	SLAY-screened peptide P5519			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTACGCTCTACTATCAGCCTTGTTATTTTCCCGCTTCTAGTTACTTCACTGAGCATCGTTAA	ATLYYQPCYFPASSYFTEHR*	-1.066	0.035271	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27170	LTIAPLSGCWDSFHVKSYYN			20	SLAY-screened peptide P5520			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCACTATCGCGCCTTTGAGCGGTTGTTGGGACAGTTTTCACGTTAAGTCGTACTATAATTAA	LTIAPLSGCWDSFHVKSYYN*	-1.066	0.020983	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27171	RPN			3	SLAY-screened peptide P5521			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCCAACTAGCGTACTACCTCCAGTTATCTCAACTAGCTGGCGACCTGCCTTCAGCCGTAA	RPN*RTTSSYLN*LATCLQP*	-1.066	0.000199	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27172	CDSSVHMTIPNDSTPNMLLM			20	SLAY-screened peptide P5522			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGATTCGAGTGTGCACATGACGATTCCTAACGACTCGACTCCGAATATGCTCTTGATGTAA	CDSSVHMTIPNDSTPNMLLM*	-1.066	0.000251	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27173	HNQYGSSHS			9	SLAY-screened peptide P5523			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAATCAGTACGGTAGTTCTCATAGCTAGGCCCGCTCCGCCCGCGAGCCGCTCCCTCTTTAA	HNQYGSSHS*ARSAREPLPL*	-1.066	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27174	RTHTHATLNSDDSHLCVLINT			21	SLAY-screened peptide P5524			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTACTCACACCCACGCTACTTTGAACAGTGATGACTCCCACCTGTGTGTCTTGATCAACACT	RTHTHATLNSDDSHLCVLINT	-1.066	0.04411	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27175	SPTRESTQICLYTI			14	SLAY-screened peptide P5525			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCCTACGCGCGAGTCGACCCAGATTTGCCTTTACACCATCTAGCACGTCACGCATACCTAA	SPTRESTQICLYTI*HVTHT*	-1.066	0.001581	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27176	CTHFEHAWTHMSASTDTASL			20	SLAY-screened peptide P5526			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCACCCATTTTGAGCATGCCTGGACTCATATGTCGGCCAGCACGGATACCGCCAGCCTCTAA	CTHFEHAWTHMSASTDTASL*	-1.065	0.002789	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27177	AVHPSPDLIAHVVYETSDQS			20	SLAY-screened peptide P5527			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGTGCACCCCAGTCCCGACCTGATCGCGCATGTTGTCTATGAGACGTCCGACCAGAGTTAA	AVHPSPDLIAHVVYETSDQS*	-1.065	0.001802	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27178	SLRSNVMTNGIQLVHRTFNT			20	SLAY-screened peptide P5528			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCTGCGTTCCAACGTTATGACCAATGGTATTCAGCTTGTGCATCGTACTTTTAATACCTAA	SLRSNVMTNGIQLVHRTFNT*	-1.065	0.010053	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27179	NMPYCNIICDNQFILIRQRTN			21	SLAY-screened peptide P5529			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATATGCCCTACTGTAATATTATTTGCGACAATCAGTTCATCTTGATCCGTCAAAGAACTAAC	NMPYCNIICDNQFILIRQRTN	-1.065	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27180	TMWAIPSTLRRWLSHKKSDS			20	SLAY-screened peptide P5530			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGATGTGGGCTATTCCTTCCACTCTGAGGCGTTGGCTGTCTCATAAGAAGTCGGATTCCTAA	TMWAIPSTLRRWLSHKKSDS*	-1.064	0.022879	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27181	IAEVFINHDINYHRCLHNVL			20	SLAY-screened peptide P5531			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGCCGAGGTTTTCATCAACCACGACATTAACTATCATCGTTGCCTCCATAATGTTCTGTAA	IAEVFINHDINYHRCLHNVL*	-1.064	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27182	NLITSLSAQLYSRVPSSTIPN			21	SLAY-screened peptide P5532			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCTTATCACTTCTTTGTCTGCCCAGCTCTACAGTAGGGTTCCGAGCAGTACGATTCCTAAC	NLITSLSAQLYSRVPSSTIPN	-1.064	0.001378	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27183	TPLHFSFTGTHIASLILDYK			20	SLAY-screened peptide P5533			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCCCCTGCACTTCAGTTTTACCGGCACGCACATCGCCTCTTTGATTCTTGATTACAAGTAA	TPLHFSFTGTHIASLILDYK*	-1.064	0.014128	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27184	NLYCSRFIIVSICIIPPTRVN			21	SLAY-screened peptide P5534			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTTGTATTGTTCAAGGTTTATAATTGTATCTATTTGCATCATTCCCCCTACTAGAGTTAAC	NLYCSRFIIVSICIIPPTRVN	-1.064	0.010404	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27185	DQLTRSLPHPTFFHSNIMHN			20	SLAY-screened peptide P5535			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCAGCTCACGAGGTCGCTCCCGCATCCTACTTTCTTCCACTCGAACATTATGCACAATTAA	DQLTRSLPHPTFFHSNIMHN*	-1.064	0.00051	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27186	RLLAHLMSQNSSRPKDQLST			20	SLAY-screened peptide P5536			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCTTCTGGCCCACCTTATGTCCCAGAATAGCTCCCGTCCTAAGGATCAGCTCAGTACCTAA	RLLAHLMSQNSSRPKDQLST*	-1.063	0.012386	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27187	VRYNKSHVFPVRLMIRNHIN			20	SLAY-screened peptide P5537			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCGCTACAATAAGAGTCATGTGTTTCCTGTCCGTCTTATGATCAGGAATCACATTAATTAA	VRYNKSHVFPVRLMIRNHIN*	-1.063	0.011904	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27188	SPLPRNTHHIPWLINR			16	SLAY-screened peptide P5538			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCCGTTGCCTCGTAATACCCATCATATCCCTTGGCTGATTAACCGGTAGATCGACCTGTAA	SPLPRNTHHIPWLINR*IDL*	-1.063	0.000623	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27189	SLACLVTNSVFFRRYKSKSCN			21	SLAY-screened peptide P5539			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCTCGCTTGCCTTGTCACCAATAGCGTGTTTTTCCGGAGGTATAAGAGCAAGAGTTGTAAC	SLACLVTNSVFFRRYKSKSCN	-1.062	4.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27190	LLCNRHLVGCNSSDGITRHI			20	SLAY-screened peptide P5540			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCTGTGCAACCGTCACCTTGTGGGTTGCAACAGCAGTGACGGTATCACCCGGCATATTTAA	LLCNRHLVGCNSSDGITRHI*	-1.062	0.016676	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27191	IQCMSPVVVPLILFTILGCLN			21	SLAY-screened peptide P5541			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCAGTGCATGTCACCAGTTGTGGTGCCCCTTATACTTTTTACAATCCTAGGCTGCCTTAAC	IQCMSPVVVPLILFTILGCLN	-1.062	0.021457	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27192	NCTYLPI			7	SLAY-screened peptide P5542			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTGCACTTATCTGCCGATCTAGAGGCGCAGCTAGCTTGTCAACCTTGTGCCCTCCCGGTAA	NCTYLPI*RRS*LVNLVPSR*	-1.062	2.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27193	PLDQNRLPGHYRFCPIYKCK			20	SLAY-screened peptide P5543			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTTGACCAGAATCGCCTTCCCGGTCACTACCGGTTTTGCCCGATTTATAAGTGTAAGTAA	PLDQNRLPGHYRFCPIYKCK*	-1.062	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27194	PCRWLPSSNTRSVTSTSFAP			20	SLAY-screened peptide P5544			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGTCGTTGGCTCCCGTCCTCCAACACTAGGTCTGTTACCTCCACTAGTTTTGCCCCGTAA	PCRWLPSSNTRSVTSTSFAP*	-1.062	0.025396	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27195	RIIQRIYLSLLALIHSNFIPN			21	SLAY-screened peptide P5545			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATTATCCAGCGCATTTACTTGAGCCTCCTCGCCCTCATTCATAGTAATTTCATACCTAAC	RIIQRIYLSLLALIHSNFIPN	-1.061	0.022044	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27196	KWNRTILHVRNSRSSTESTG			20	SLAY-screened peptide P5546			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGTGGAATAGGACCATCCTCCATGTTCGCAATTCTAGGAGTAGTACTGAGTCCACTGGTTAA	KWNRTILHVRNSRSSTESTG*	-1.061	0.000155	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27197	HMTCRDTILTPSLV			14	SLAY-screened peptide P5547			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATATGACCTGTCGGGATACTATCCTGACCCCGTCTTTGGTCTAGCCCGTCCCCTATCGGTAA	HMTCRDTILTPSLV*PVPYR*	-1.061	0.000289	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27198	TGDVTAPRPNGFGLQYFIYI			20	SLAY-screened peptide P5548			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGGGGATGTTACCGCGCCCAGGCCTAATGGTTTCGGTCTTCAGTACTTTATTTACATTTAA	TGDVTAPRPNGFGLQYFIYI*	-1.061	0.031849	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27199	ELSCRTPVHCPLYPGLIHRC			20	SLAY-screened peptide P5549			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCTTTCCTGTCGCACCCCCGTGCATTGCCCCCTGTACCCGGGCCTCATCCACCGTTGCTAA	ELSCRTPVHCPLYPGLIHRC*	-1.061	0.009045	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27200	VCAPADLRTSSKTNYNSVPE			20	SLAY-screened peptide P5550			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTGCGCCCCTGCTGACCTTCGCACTTCTAGCAAGACCAACTACAACTCGGTGCCCGAGTAA	VCAPADLRTSSKTNYNSVPE*	-1.06	0.005938	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27201	RPTSSRLMLNCIPGSYNPNW			20	SLAY-screened peptide P5551			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCTACTAGCTCTCGCCTCATGCTCAATTGTATTCCCGGGTCCTACAATCCTAACTGGTAA	RPTSSRLMLNCIPGSYNPNW*	-1.06	0.004022	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27202	MTCVPRWGYHPIMGTPIYSR			20	SLAY-screened peptide P5552			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGACCTGCGTGCCCCGCTGGGGTTATCACCCTATCATGGGCACTCCCATTTATAGCCGTTAA	MTCVPRWGYHPIMGTPIYSR*	-1.06	0.003033	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27203	AAMGSNLQPEYLTPIRHPHH			20	SLAY-screened peptide P5553			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGCTATGGGGTCCAACCTTCAGCCCGAGTATCTCACGCCCATTCGGCACCCCCACCATTAA	AAMGSNLQPEYLTPIRHPHH*	-1.06	0.01817	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27204	WSCDMNRPISPLGYIPQYFH			20	SLAY-screened peptide P5554			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTCGTGTGACATGAACCGGCCTATCTCCCCGCTGGGCTATATCCCGCAGTACTTTCATTAA	WSCDMNRPISPLGYIPQYFH*	-1.06	0.009444	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27205	ATGGCDPLLYHTIWSRRIF			19	SLAY-screened peptide P5555			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACTGGTGGCTGTGACCCCCTCCTTTACCATACCATTTGGAGCAGGCGGATTTTCTAGTAA	ATGGCDPLLYHTIWSRRIF**	-1.06	0.002232	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27206	NHICQYRLSHRARSRKRMNR			20	SLAY-screened peptide P5556			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACCACATTTGCCAGTACCGCCTTTCGCACCGGGCGCGTAGCCGTAAGCGTATGAATCGGTAA	NHICQYRLSHRARSRKRMNR*	-1.06	0.019154	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27207	WNVRARLWRIALSVLHILRT			20	SLAY-screened peptide P5557			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGAATGTGCGTGCCCGCCTGTGGCGTATCGCCTTGTCCGTGCTCCACATCCTTCGTACCTAA	WNVRARLWRIALSVLHILRT*	-1.06	0.000303	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27208	PGNPLVIIHTFLSSSPPGTS			20	SLAY-screened peptide P5558			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGCAACCCCTTGGTCATCATCCATACCTTTCTGAGTAGCAGTCCCCCCGGTACTAGCTAA	PGNPLVIIHTFLSSSPPGTS*	-1.06	0.040428	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27209	CRLRPRLFRASGVRVRRGRPN			21	SLAY-screened peptide P5559			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGCTTGCGCCCGAGACTTTTTCGTGCATCCGGTGTACGCGTACGCCGTGGACGTCCTAAC	CRLRPRLFRASGVRVRRGRPN	-1.059	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27210	CVPVSHLG			8	SLAY-screened peptide P5560			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGTGCCGGTTAGCCATCTTGGGTAGCTCTTTATTGACGCTATCAACTTGCGGGACGTGTAA	CVPVSHLG*LFIDAINLRDV*	-1.059	0.006557	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27211	PVPPIHTSFPDRYTRYRSTY			20	SLAY-screened peptide P5561			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGTCCCCCCGATTCATACTAGCTTTCCGGATCGCTATACTCGGTACCGGTCCACCTATTAA	PVPPIHTSFPDRYTRYRSTY*	-1.058	0.00406	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27212	LPPCTVAPYNSLNSRSSGHG			20	SLAY-screened peptide P5562			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCCCCCTGTACGGTTGCCCCGTATAACTCGCTCAATTCGCGTAGTAGCGGTCACGGTTAA	LPPCTVAPYNSLNSRSSGHG*	-1.058	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27213	CAVMCPGLHNLAHYVYPPCF			20	SLAY-screened peptide P5563			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCGGTCATGTGTCCTGGTCTGCACAATCTGGCTCATTATGTGTATCCTCCGTGTTTCTAA	CAVMCPGLHNLAHYVYPPCF*	-1.058	0.017721	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27214	SLSSSPRPHSFVNSLGHSMC			20	SLAY-screened peptide P5564			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCTTAGCAGTAGCCCTCGCCCCCACTCTTTTGTTAACTCTCTTGGTCATTCCATGTGTTAA	SLSSSPRPHSFVNSLGHSMC*	-1.058	0.006355	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27215	HQRRTNPTTPRLAMHFSMRY			20	SLAY-screened peptide P5565			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCAGCGTCGCACTAATCCGACCACGCCCCGTCTGGCTATGCATTTCTCCATGCGCTACTAA	HQRRTNPTTPRLAMHFSMRY*	-1.058	0.00014	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27216	KQPVPSCIHKYSASLGADFL			20	SLAY-screened peptide P5566			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCAGCCTGTGCCCTCTTGTATCCATAAGTATTCGGCGTCGCTTGGCGCCGACTTTCTTTAA	KQPVPSCIHKYSASLGADFL*	-1.058	0.006489	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27217	DSLNQPLVRSNFMPHNQRS			19	SLAY-screened peptide P5567			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTCGCTCAATCAGCCCCTCGTTCGGTCCAACTTTATGCCCCATAACCAGCGCAGTTAGTAA	DSLNQPLVRSNFMPHNQRS**	-1.058	0.02709	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27218	IPASTPTNALFAHIRCLLRR			20	SLAY-screened peptide P5568			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTCCCGCCTCCACGCCCACCAACGCCCTCTTTGCCCATATCCGTTGCCTTTTGCGCCGCTAA	IPASTPTNALFAHIRCLLRR*	-1.058	0.010189	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27219	PVPKHFNRNLRVR			13	SLAY-screened peptide P5569			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGTTCCGAAGCATTTCAATCGTAACCTTCGTGTTAGGTAGGCCGGGCTTCTTAACCCGTAA	PVPKHFNRNLRVR*AGLLNP*	-1.057	3.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27220	RVCKAF			6	SLAY-screened peptide P5570			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGTGTGTAAGGCGTTCTAGATCATTAGTTCGTTCCTTCCTTGCAAGACCGTCATTGAGTAA	RVCKAF*IISSFLPCKTVIE*	-1.057	0.002301	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27221	LFSPYRWYYNYLHPTLSGAI			20	SLAY-screened peptide P5571			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTTTTCCCCCTATCGTTGGTACTATAACTATCTGCATCCCACCCTGAGTGGCGCGATCTAA	LFSPYRWYYNYLHPTLSGAI*	-1.057	0.045281	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27222	LILNLAPLYYTVFANLSSIN			20	SLAY-screened peptide P5572			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGATTCTCAATCTGGCCCCTTTGTACTACACTGTCTTCGCGAACTTGTCCAGTATTAACTAA	LILNLAPLYYTVFANLSSIN*	-1.057	0.014861	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27223	CALDESRTYRDNEYLVYQWY			20	SLAY-screened peptide P5573			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTGCGTTGGATGAGTCCCGTACCTATCGGGATAACGAGTATCTTGTGTACCAGTGGTATTAA	CALDESRTYRDNEYLVYQWY*	-1.057	0.0062	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27224	FPYSVLLYCVEQNIGTTMPTN			21	SLAY-screened peptide P5574			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCCTTACAGTGTTCTCCTGTATTGTGTCGAGCAGAACATTGGTACTACCATGCCAACTAAC	FPYSVLLYCVEQNIGTTMPTN	-1.057	0.028469	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27225	SSSYTSSKGPTPMCVIRVEQ			20	SLAY-screened peptide P5575			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCAGTTCTTATACCTCGAGTAAGGGTCCTACGCCTATGTGCGTGATCCGCGTTGAGCAGTAA	SSSYTSSKGPTPMCVIRVEQ*	-1.057	0.029188	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27226	TLNAIANCLMLIYFIFVMNL			20	SLAY-screened peptide P5576			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTCAATGCGATCGCCAATTGTCTGATGTTGATCTATTTTATTTTTGTTATGAACCTCTAA	TLNAIANCLMLIYFIFVMNL*	-1.057	0.016914	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27227	FRSLEALMPTNYYNDDTASA			20	SLAY-screened peptide P5577			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCGCTCTTTGGAGGCCCTTATGCCTACCAACTACTACAACGATGATACCGCGAGTGCCTAA	FRSLEALMPTNYYNDDTASA*	-1.057	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27228	CLSSMWKWCSIISAYNIAVV			20	SLAY-screened peptide P5578			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCTGTCCTCTATGTGGAAGTGGTGTTCGATCATTTCTGCCTACAACATCGCGGTTGTGTAA	CLSSMWKWCSIISAYNIAVV*	-1.057	0.000243	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27229	LHTLYYFLSRLYGFFFQVRK			20	SLAY-screened peptide P5579			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGCATACTCTGTATTACTTCTTGTCTCGCTTGTACGGCTTCTTCTTTCAGGTGCGGAAGTAA	LHTLYYFLSRLYGFFFQVRK*	-1.057	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27230	VSCAYICDNQYLWLFPLALL			20	SLAY-screened peptide P5580			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTCTTGCGCTTATATCTGCGACAACCAGTATCTGTGGCTCTTCCCCCTCGCTTTGCTCTAA	VSCAYICDNQYLWLFPLALL*	-1.057	0.002123	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27231	PYLLCVNRPVCFRVSAGALL			20	SLAY-screened peptide P5581			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTATCTCCTTTGCGTTAACCGTCCTGTTTGCTTCCGTGTCAGCGCCGGTGCTCTCCTCTAA	PYLLCVNRPVCFRVSAGALL*	-1.057	0.005458	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27232	TIPQRWRTFFTPCYCDPQFY			20	SLAY-screened peptide P5582			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTATCCCCCAGCGGTGGCGTACGTTTTTTACTCCTTGCTACTGTGATCCCCAGTTCTATTAA	TIPQRWRTFFTPCYCDPQFY*	-1.057	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27233	PTDPRALMEVAKVTRKSDDS			20	SLAY-screened peptide P5583			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTACCGACCCGCGCGCTTTGATGGAGGTGGCTAAGGTTACTAGGAAGAGCGACGATAGTTAA	PTDPRALMEVAKVTRKSDDS*	-1.056	1.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27234	HNYGCNLHTDWTDLARPEYL			20	SLAY-screened peptide P5584			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAATTACGGCTGCAACTTGCATACCGATTGGACGGATCTCGCCCGTCCTGAGTACTTGTAA	HNYGCNLHTDWTDLARPEYL*	-1.056	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27235	HCSTAPRFLMIMNPHYTSRI			20	SLAY-screened peptide P5585			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTGCTCTACCGCGCCTCGTTTCCTCATGATTATGAACCCTCATTACACTTCGCGGATTTAA	HCSTAPRFLMIMNPHYTSRI*	-1.056	0.005541	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27236	PLESMPEVSPPLCVPHPILL			20	SLAY-screened peptide P5586			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTGGAGTCTATGCCTGAGGTTTCCCCCCCGCTCTGTGTCCCTCATCCCATCCTGTTGTAA	PLESMPEVSPPLCVPHPILL*	-1.056	3.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27237	HVSDADHSSIRFCPIFSVTL			20	SLAY-screened peptide P5587			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGTTTCCGACGCTGACCATTCTTCCATTCGCTTTTGCCCTATCTTCTCGGTTACTCTCTAA	HVSDADHSSIRFCPIFSVTL*	-1.056	0.000374	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27238	ACPTDIKDRGTPLLIFLRGT			20	SLAY-screened peptide P5588			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTGCCCGACCGACATTAAGGACCGTGGCACTCCGCTCTTGATCTTTCTTCGTGGGACTTAA	ACPTDIKDRGTPLLIFLRGT*	-1.056	0.000749	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27239	PRYIPFCFVPLDVYSESKAS			20	SLAY-screened peptide P5589			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCGGTACATTCCTTTTTGCTTCGTCCCCTTGGATGTGTATTCTGAGAGTAAGGCTAGTTAA	PRYIPFCFVPLDVYSESKAS*	-1.056	0.047155	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27240	VFISCKPLATNHLVVFPSSA			20	SLAY-screened peptide P5590			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTTCATTTCTTGTAAGCCTCTTGCCACGAATCATCTTGTCGTGTTCCCCTCGTCGGCGTAA	VFISCKPLATNHLVVFPSSA*	-1.056	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27241	AHLAYRSACQPSFFLTSVDS			20	SLAY-screened peptide P5591			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCATCTTGCCTACCGTAGTGCGTGTCAGCCTAGTTTTTTCTTGACTAGTGTTGACTCGTAA	AHLAYRSACQPSFFLTSVDS*	-1.055	0.031027	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27242	QRSGSFLLTTYGTVRRNFHL			20	SLAY-screened peptide P5592			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCGCTCCGGGTCCTTCCTGTTGACTACGTACGGTACCGTGAGGCGGAACTTCCATCTCTAA	QRSGSFLLTTYGTVRRNFHL*	-1.055	0.003435	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27243	SLCLN			5	SLAY-screened peptide P5593			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCTGTGCCTCAATTAGGCTCGCGAGTCCGTGTACCAGGTGAATATGATTATCGGGAACTAA	SLCLN*ARESVYQVNMIIGN*	-1.055	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27244	IYDCCMRWKNLWHSLLCSPV			20	SLAY-screened peptide P5594			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTTACGACTGCTGTATGCGCTGGAAGAACCTTTGGCACTCGCTTTTGTGCTCTCCTGTTTAA	IYDCCMRWKNLWHSLLCSPV*	-1.055	7.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27245	FISPKVHIIYDVYKGDKNSS			20	SLAY-screened peptide P5595			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTATCTCGCCCAAGGTGCACATCATTTACGATGTGTATAAGGGTGATAAGAACTCCTCCTAA	FISPKVHIIYDVYKGDKNSS*	-1.055	0.000776	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27246	MQGCHSLEAMPTMTGSIISI			20	SLAY-screened peptide P5596			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCAGGGGTGTCATTCGCTGGAGGCCATGCCCACCATGACCGGCTCCATCATTTCTATTTAA	MQGCHSLEAMPTMTGSIISI*	-1.054	0.025849	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27247	CHDHAHSLLILGSNTHSFI			19	SLAY-screened peptide P5597			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCATGACCACGCCCACTCCCTTCTCATTCTGGGTAGTAATACGCATAGTTTTATTTAGTAA	CHDHAHSLLILGSNTHSFI**	-1.054	0.023417	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27248	PCEHAKLLYKIVRQYFPSF			19	SLAY-screened peptide P5598			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTGTGAGCATGCCAAGTTGCTGTACAAGATCGTCAGGCAGTATTTCCCCTCGTTTTAGTAA	PCEHAKLLYKIVRQYFPSF**	-1.054	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27249	IAAPIWICFDRCFSLRSATH			20	SLAY-screened peptide P5599			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGCGGCCCCCATTTGGATCTGTTTTGACCGTTGTTTTAGTCTTCGGTCTGCTACTCATTAA	IAAPIWICFDRCFSLRSATH*	-1.054	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27250	PPLCPPVSYMLITDRLGMIP			20	SLAY-screened peptide P5600			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCGCTCTGTCCGCCCGTCTCCTACATGCTGATCACTGATCGCCTCGGCATGATCCCTTAA	PPLCPPVSYMLITDRLGMIP*	-1.053	0.018134	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27251	RIRDTLSNTCNKILYMTGKI			20	SLAY-screened peptide P5601			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTATCCGGGATACTCTTTCTAATACTTGTAACAAGATTTTGTATATGACTGGTAAGATTTAA	RIRDTLSNTCNKILYMTGKI*	-1.053	0.019273	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27252	PFDWVNGGLSCQKHRLNPAL			20	SLAY-screened peptide P5602			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTTCGACTGGGTTAATGGCGGTCTCTCCTGCCAGAAGCACCGCCTTAATCCGGCTCTGTAA	PFDWVNGGLSCQKHRLNPAL*	-1.053	3.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27253	PVRTRPTGCSFPSAFGCGYC			20	SLAY-screened peptide P5603			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGTGCGCACTCGTCCGACTGGGTGTTCTTTTCCTTCTGCTTTCGGTTGTGGCTATTGTTAA	PVRTRPTGCSFPSAFGCGYC*	-1.053	0.018034	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27254	PCPRSLIFLT			10	SLAY-screened peptide P5604			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGTGCCCTCGGTCGTTGATTTTTCTTACCTAGTGCTGCAATACGCTGATCCGTTCTTCTTAA	PCPRSLIFLT*CCNTLIRSS*	-1.052	0.031445	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27255	TEDCDARKYMTYILMYHPNL			20	SLAY-screened peptide P5605			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGAGGATTGCGATGCGAGGAAGTACATGACTTATATCCTCATGTATCACCCGAATTTGTAA	TEDCDARKYMTYILMYHPNL*	-1.052	0.012715	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27256	CHPGLIYIPTMFQPDYNWAN			20	SLAY-screened peptide P5606			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCATCCCGGGCTTATCTATATCCCGACCATGTTTCAGCCCGATTACAACTGGGCGAATTAA	CHPGLIYIPTMFQPDYNWAN*	-1.052	0.00348	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27257	HNQALHTFTLLYEVVWHLGLT			21	SLAY-screened peptide P5607			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATAATCAGGCCCTGCACACTTTTACGTTATTATATGAAGTCGTCTGGCATCTAGGACTAACT	HNQALHTFTLLYEVVWHLGLT	-1.052	0.000713	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27258	WAPMLQSCASVHFFGDARPT			20	SLAY-screened peptide P5608			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGGCGCCCATGCTCCAGTCCTGTGCTTCCGTTCATTTTTTCGGTGACGCTCGTCCCACTTAA	WAPMLQSCASVHFFGDARPT*	-1.052	0.008072	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27259	AAAVSETITPLLRCCHTSCA			20	SLAY-screened peptide P5609			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCGGCGGTGAGCGAGACCATTACCCCTCTTCTCAGGTGCTGCCATACTAGTTGCGCCTAA	AAAVSETITPLLRCCHTSCA*	-1.052	0.049486	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27260	PALLKFFYIIASCMRAALRC			20	SLAY-screened peptide P5610			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCGCTCCTCAAGTTTTTCTATATCATTGCCAGTTGCATGCGTGCCGCGCTGCGTTGTTAA	PALLKFFYIIASCMRAALRC*	-1.051	0.000586	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27261	CLYLNNNRFTFYTPTFL			17	SLAY-screened peptide P5611			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCTTTACCTGAATAATAATCGTTTCACTTTTTACACTCCTACGTTTCTGTAGCAGATGTAA	CLYLNNNRFTFYTPTFL*QM*	-1.051	0.000227	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27262	RCTPDLTIFTHHFNAYTIALT			21	SLAY-screened peptide P5612			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGCACGCCGGATCTGACCATTTTCACTCACCACTTCAACGCCTATACAATAGCCTTAACT	RCTPDLTIFTHHFNAYTIALT	-1.051	0.014488	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27263	PIQWTLKALRHWYFTNRTC			19	SLAY-screened peptide P5613			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGATTCAGTGGACCCTTAAGGCCCTTAGGCACTGGTATTTTACCAACCGGACCTGTTAGTAA	PIQWTLKALRHWYFTNRTC**	-1.051	0.000266	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27264	TDHTTALPDATATCLYSAFT			20	SLAY-screened peptide P5614			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGACCACACCACTGCTCTGCCGGATGCTACTGCTACCTGCCTGTATTCTGCGTTCACGTAA	TDHTTALPDATATCLYSAFT*	-1.051	0.04233	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27265	PHGCEHNYIPLYPFFTGAFL			20	SLAY-screened peptide P5615			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCACGGCTGCGAGCACAATTACATCCCCCTTTACCCTTTTTTCACCGGTGCGTTCCTCTAA	PHGCEHNYIPLYPFFTGAFL*	-1.05	0.000285	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27266	SCLFASRASVPLHNMYFATI			20	SLAY-screened peptide P5616			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTGCCTCTTTGCCTCCCGCGCTTCTGTCCCTCTTCACAATATGTATTTCGCTACCATCTAA	SCLFASRASVPLHNMYFATI*	-1.05	0.01075	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27267	DNCWDPDAYRNSANYHILLF			20	SLAY-screened peptide P5617			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATAACTGTTGGGACCCGGACGCCTACCGTAATAGCGCTAATTATCATATTCTCCTTTTCTAA	DNCWDPDAYRNSANYHILLF*	-1.05	0.00107	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27268	VDPLTIQYKTDNATHASMET			20	SLAY-screened peptide P5618			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGACCCTCTTACGATCCAGTACAAGACCGACAATGCTACGCACGCCTCTATGGAGACCTAA	VDPLTIQYKTDNATHASMET*	-1.05	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27269	TVWQILKCIYLLHCYNLFFG			20	SLAY-screened peptide P5619			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTCTGGCAGATTCTTAAGTGCATTTACCTTCTGCATTGCTATAATTTGTTCTTTGGCTAA	TVWQILKCIYLLHCYNLFFG*	-1.049	0.0034	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27270	GFA			3	SLAY-screened peptide P5620			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTTTGCGTAGCTGCGCCTGGTCAACTGGCTTAAGCTTGCGAATGCGAAGCGCACCGGTTAA	GFA*LRLVNWLKLANAKRTG*	-1.049	0.024285	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27271	DCIKPQLPNLYNIVY			15	SLAY-screened peptide P5621			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTGTATTAAGCCCCAGCTGCCTAACCTCTACAACATTGTGTACTAGTGCCTTCTTGATTAA	DCIKPQLPNLYNIVY*CLLD*	-1.049	0.014529	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27272	EPYHPTLRYDGQHLPFTYCY			20	SLAY-screened peptide P5622			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCCGTACCACCCCACCCTTCGGTATGATGGCCAGCACTTGCCGTTCACTTATTGCTACTAA	EPYHPTLRYDGQHLPFTYCY*	-1.049	0.000179	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27273	TNIARTMHNYIRTTLDNNFIN			21	SLAY-screened peptide P5623			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAACATCGCGCGGACTATGCACAATTACATCCGCACTACTTTGGACAACAATTTCATTAAC	TNIARTMHNYIRTTLDNNFIN	-1.049	3.2e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27274	RKGYNHTLHFCILIRFEYYY			20	SLAY-screened peptide P5624			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAAGGGCTACAATCACACCCTGCACTTCTGTATCCTTATCCGGTTCGAGTACTACTACTAA	RKGYNHTLHFCILIRFEYYY*	-1.049	0.009608	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27275	PQDNYSAFHPYGHCMPNWSL			20	SLAY-screened peptide P5625			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCAGGATAATTACTCCGCTTTCCATCCGTATGGCCACTGCATGCCCAATTGGTCTCTGTAA	PQDNYSAFHPYGHCMPNWSL*	-1.049	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27276	SCRYMAPPIATDRNLTYFSM			20	SLAY-screened peptide P5626			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCTGCCGTTATATGGCTCCCCCCATTGCTACGGACCGTAACCTTACGTACTTTAGCATGTAA	SCRYMAPPIATDRNLTYFSM*	-1.049	0.012505	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27277	TCPNAQLPDEFLFV			14	SLAY-screened peptide P5627			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCTGTCCCAACGCTCAGCTTCCGGACGAATTCTTATTTGTATAGGCACTTGGTCAATTAACT	TCPNAQLPDEFLFV*ALGQLT	-1.048	0.001587	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27278	YSTQYGDGDLPVDRTESRAD			20	SLAY-screened peptide P5628			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTCTACGCAGTACGGTGACGGGGATTTGCCTGTTGACCGTACGGAGTCTCGGGCGGATTAA	YSTQYGDGDLPVDRTESRAD*	-1.048	0.044542	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27279	RTWSRDRDPHEPSSIFLIFL			20	SLAY-screened peptide P5629			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTACGTGGTCCCGCGACCGCGACCCCCACGAGCCTTCTAGTATCTTCCTGATCTTCCTCTAA	RTWSRDRDPHEPSSIFLIFL*	-1.048	0.035682	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27280	TLQGYSCVPRPNGRLHARND			20	SLAY-screened peptide P5630			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCTGCAGGGTTACTCCTGCGTGCCGCGCCCTAACGGTCGTCTCCATGCGCGCAACGATTAA	TLQGYSCVPRPNGRLHARND*	-1.048	0.026325	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27281	VHRATPCLSRPPGMLTQPF			19	SLAY-screened peptide P5631			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCATCGCGCCACGCCCTGCTTGAGCCGGCCGCCCGGCATGCTCACTCAGCCTTTCTAGTAA	VHRATPCLSRPPGMLTQPF**	-1.048	0.02764	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27282	HTVRPTVIVNCRNPSYELND			20	SLAY-screened peptide P5632			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACCGTCCGCCCGACCGTTATCGTTAATTGTCGTAATCCTAGTTACGAGCTCAATGATTAA	HTVRPTVIVNCRNPSYELND*	-1.047	4.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27283	PLLSLSVSNLYIVLYNFLKR			20	SLAY-screened peptide P5633			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCCTTTCTCTTTCCGTCTCTAATTTGTATATCGTCCTGTATAACTTCTTGAAGAGGTAA	PLLSLSVSNLYIVLYNFLKR*	-1.047	0.002109	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27284	PSASSAYHRYHSRSCLLFF			19	SLAY-screened peptide P5634			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTCGGCTTCCAGTGCCTACCACCGGTATCACAGCCGGAGTTGTTTGCTCTTTTTTTAGTAA	PSASSAYHRYHSRSCLLFF**	-1.047	0.000108	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27285	ACFSHLTLVINSDQPIYVAL			20	SLAY-screened peptide P5635			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTGTTTCTCGCATTTGACTCTCGTCATCAACAGTGATCAGCCTATCTACGTCGCGCTTTAA	ACFSHLTLVINSDQPIYVAL*	-1.047	0.000786	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27286	NNYNWPFILIRAPIPRARSIN			21	SLAY-screened peptide P5636			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACAACTATAACTGGCCGTTTATCTTAATACGCGCACCCATCCCCCGCGCTCGGTCAATTAAC	NNYNWPFILIRAPIPRARSIN	-1.046	0.024428	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27287	PVTLLFPFRRPCSHFDAMRP			20	SLAY-screened peptide P5637			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGTCACCCTGTTGTTCCCCTTCCGGAGGCCCTGTTCGCACTTCGATGCTATGCGTCCCTAA	PVTLLFPFRRPCSHFDAMRP*	-1.046	0.009643	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27288	LLMRVSRCLVVYTLISMFMS			20	SLAY-screened peptide P5638			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCTGATGCGTGTTAGCAGGTGTTTGGTTGTGTACACGCTGATCTCTATGTTTATGAGTTAA	LLMRVSRCLVVYTLISMFMS*	-1.046	0.000668	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27289	RSTAAGTRSSSPLTWWCCIH			20	SLAY-screened peptide P5639			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCAGTACCGCTGCGGGGACCCGGTCCTCCTCTCCTCTCACCTGGTGGTGTTGCATCCACTAA	RSTAAGTRSSSPLTWWCCIH*	-1.046	0.000477	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27290	SFYHPSYTPLIHFVDTYHAN			20	SLAY-screened peptide P5640			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTTCTATCACCCGTCTTACACCCCCCTTATCCATTTTGTGGACACGTATCACGCTAATTAA	SFYHPSYTPLIHFVDTYHAN*	-1.046	0.005849	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27291	VVHNLYRRLCLLRKSDSAY			19	SLAY-screened peptide P5641			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCGTGCACAACCTCTACCGCAGGCTGTGCCTCCTCAGGAAGTCTGATTCGGCCTACTAGTAA	VVHNLYRRLCLLRKSDSAY**	-1.046	0.000833	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27292	LLCARKCLRLFDPYCGMWEI			20	SLAY-screened peptide P5642			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTGCTCTGTGCCCGTAAGTGCCTCAGGCTTTTTGACCCCTATTGCGGCATGTGGGAGATCTAA	LLCARKCLRLFDPYCGMWEI*	-1.046	0.036464	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27293	PRYYDTVNMHCLPFYWSNSR			20	SLAY-screened peptide P5643			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGTTATTATGATACGGTTAACATGCACTGCCTGCCGTTTTACTGGAGCAACTCGCGGTAA	PRYYDTVNMHCLPFYWSNSR*	-1.045	9.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27294	LPTHAIITLTTATLECNSYV			20	SLAY-screened peptide P5644			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCTACCCATGCGATTATTACGCTGACGACTGCTACTTTGGAGTGCAATTCGTACGTCTAA	LPTHAIITLTTATLECNSYV*	-1.045	0.003988	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27295	VHRDINGLCIDTHDTISLII			20	SLAY-screened peptide P5645			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCACCGCGATATCAACGGCTTGTGTATTGACACGCATGATACGATTTCTTTGATTATCTAA	VHRDINGLCIDTHDTISLII*	-1.045	0.02626	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27296	CPAALLVRASPVLYFRPDDD			20	SLAY-screened peptide P5646			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCTGCCGCCCTTCTGGTGCGTGCCAGTCCCGTCCTGTACTTTAGGCCTGACGACGACTAA	CPAALLVRASPVLYFRPDDD*	-1.045	0.015901	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27297	LCCPTYSQKRSMCNHLLVEI			20	SLAY-screened peptide P5647			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTGCTGTCCCACGTACAGTCAGAAGCGCAGTATGTGCAATCACCTCCTTGTGGAGATTTAA	LCCPTYSQKRSMCNHLLVEI*	-1.044	0.032091	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27298	GMHYRHSRFPLRAGTCLHLV			20	SLAY-screened peptide P5648			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTATGCACTATAGGCATTCTCGGTTCCCGTTGCGTGCGGGCACTTGCCTCCACCTTGTGTAA	GMHYRHSRFPLRAGTCLHLV*	-1.044	0.035618	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27299	AGCQF			5	SLAY-screened peptide P5649			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGGGTGTCAGTTCTAGCCCATGTGGATTTGCCAGTTTTGTTTCCACGCGTTCTACACCTAA	AGCQF*PMWICQFCFHAFYT*	-1.044	0.007208	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27300	SEICKYSDLGNFLCLCRSYL			20	SLAY-screened peptide P5650			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGAGATCTGTAAGTACAGTGATCTTGGCAATTTTCTTTGCCTCTGTCGCTCCTATCTCTAA	SEICKYSDLGNFLCLCRSYL*	-1.044	0.000108	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27301	DPSTSMKCSPYDRILRLAKH			20	SLAY-screened peptide P5651			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCCGAGTACGTCTATGAAGTGCTCTCCCTACGACAGGATTTTGCGTCTCGCGAAGCACTAA	DPSTSMKCSPYDRILRLAKH*	-1.044	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27302	SDPHLHMFYPAPYLCPNASF			20	SLAY-screened peptide P5652			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGACCCTCATCTTCACATGTTCTATCCCGCGCCGTATCTGTGCCCTAATGCGAGCTTCTAA	SDPHLHMFYPAPYLCPNASF*	-1.044	0.000911	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27303	PRDCQWASMYDVT			13	SLAY-screened peptide P5653			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCGTGACTGTCAGTGGGCCTCTATGTACGATGTTACGTAGAAGTAACTAAGATGATTTAAC	PRDCQWASMYDVT*K*LR*FN	-1.044	0.028447	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27304	PRLQFGYSNRDYHAFCGKFR			20	SLAY-screened peptide P5654			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCGCTTGCAGTTCGGTTATTCGAACAGGGATTATCATGCGTTCTGCGGTAAGTTCCGTTAA	PRLQFGYSNRDYHAFCGKFR*	-1.044	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27305	LFSNWAPFRLLTRTPTAPTCN			21	SLAY-screened peptide P5655			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTTCTCTAACTGGGCTCCCTTCCGTCTCCTCACTCGGACCCCCACGGCGCCTACTTGTAAC	LFSNWAPFRLLTRTPTAPTCN	-1.044	0.00148	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27306	KQDSHNLPCNPLKHLYILTTN			21	SLAY-screened peptide P5656			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCAGGATTCTCACAATTTGCCGTGTAATCCCCTCAAGCACCTTTACATACTAACTACTAAC	KQDSHNLPCNPLKHLYILTTN	-1.044	0.021672	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27307	PDHDYDVTCTHMFSFPYQLV			20	SLAY-screened peptide P5657			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGACCACGACTACGACGTCACCTGTACCCATATGTTTTCGTTCCCTTATCAGCTGGTTTAA	PDHDYDVTCTHMFSFPYQLV*	-1.044	0.040381	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27308	PRAIRCHPSIYRIPYCPHWH			20	SLAY-screened peptide P5658			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGGGCCATTAGGTGCCACCCTTCGATTTATCGTATTCCGTACTGCCCTCACTGGCACTAA	PRAIRCHPSIYRIPYCPHWH*	-1.043	0.041945	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27309	AYYNHGRSSYFRYNDPCSGY			20	SLAY-screened peptide P5659			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTATTATAATCATGGGCGTAGCTCGTACTTCCGCTACAATGATCCCTGCAGTGGTTACTAA	AYYNHGRSSYFRYNDPCSGY*	-1.043	0.01901	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27310	HTVDHGPRAPFIIMLTLPSTN			21	SLAY-screened peptide P5660			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACCGTGGACCATGGGCCTAGAGCCCCCTTCATTATAATGTTAACTTTACCATCCACTAAC	HTVDHGPRAPFIIMLTLPSTN	-1.043	0.000844	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27311	LNGTYENNNYRTIIPYFHFC			20	SLAY-screened peptide P5661			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTAACGGCACCTACGAGAATAATAATTACCGGACCATCATTCCGTACTTTCACTTCTGCTAA	LNGTYENNNYRTIIPYFHFC*	-1.043	1.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27312	VSDACYWPHAYYHRNESCCI			20	SLAY-screened peptide P5662			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGTCTGACGCGTGCTATTGGCCCCATGCTTACTACCACCGCAATGAGAGTTGTTGTATTTAA	VSDACYWPHAYYHRNESCCI*	-1.042	0.000226	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27313	CPIITWGYSMLSP			13	SLAY-screened peptide P5663			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCGATTATTACTTGGGGTTACAGTATGTTGTCGCCCTAGGGGAGGTTGTCTATGTGCTAA	CPIITWGYSMLSP*GRLSMC*	-1.042	7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27314	RSCPGPMRSTPHLNYLNLMI			20	SLAY-screened peptide P5664			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTCGTGCCCCGGCCCGATGCGCTCTACCCCTCATCTGAATTACCTTAACTTGATGATCTAA	RSCPGPMRSTPHLNYLNLMI*	-1.042	0.026468	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27315	PYYTLYPGMVCHSYDMMIFP			20	SLAY-screened peptide P5665			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTACTATACGCTGTACCCCGGCATGGTTTGCCACAGTTACGACATGATGATTTTTCCTTAA	PYYTLYPGMVCHSYDMMIFP*	-1.042	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27316	PPTSIKCDGTDW			12	SLAY-screened peptide P5666			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCCACGAGTATCAAGTGCGACGGCACTGACTGGTAGACCAACTTCCCTTACGACAACTAA	PPTSIKCDGTDW*TNFPYDN*	-1.041	0.010852	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27317	SDYTPMRTYVCSVIP			15	SLAY-screened peptide P5667			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTGACTATACCCCGATGCGTACGTATGTTTGTTCGGTTATTCCCTAGATCACCTTCCTCTAA	SDYTPMRTYVCSVIP*ITFL*	-1.041	0.01303	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27318	NFARIRCWHYSSGMYGHSNY			20	SLAY-screened peptide P5668			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTTCGCCCGTATTCGTTGCTGGCATTATAGCAGCGGTATGTACGGTCATTCGAATTATTAA	NFARIRCWHYSSGMYGHSNY*	-1.041	0.041437	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27319	CDPTLPPIQLCYRCYPTLAQ			20	SLAY-screened peptide P5669			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGACCCCACGCTGCCTCCGATTCAGTTGTGTTACCGTTGCTATCCCACTTTGGCGCAGTAA	CDPTLPPIQLCYRCYPTLAQ*	-1.041	0.028605	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27320	TQLRPDRSPHSPHQSPTWSQ			20	SLAY-screened peptide P5670			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGCAGTTGCGGCCTGACCGCTCTCCTCATTCCCCTCACCAGAGCCCTACCTGGAGCCAGTAA	TQLRPDRSPHSPHQSPTWSQ*	-1.041	0.021321	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27321	WSSPDTRLFILPGVHKSVMF			20	SLAY-screened peptide P5671			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTCTTCCCCGGACACTAGGCTGTTTATCTTGCCCGGGGTTCATAAGAGTGTTATGTTTTAA	WSSPDTRLFILPGVHKSVMF*	-1.041	0.000392	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27322	RPCNHYMLHRSLTAVNSMIA			20	SLAY-screened peptide P5672			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGGCCCTGCAATCACTATATGCTCCATCGTTCGCTCACCGCTGTCAATTCTATGATTGCGTAA	RPCNHYMLHRSLTAVNSMIA*	-1.04	0.036127	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27323	DPLTKFIQLSLVNHHNDNAC			20	SLAY-screened peptide P5673			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCCCTTGACGAAGTTTATCCAGCTTAGTCTTGTGAATCATCACAACGACAATGCCTGTTAA	DPLTKFIQLSLVNHHNDNAC*	-1.04	0.008347	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27324	PSSDNAQCFYHHNHISSSFS			20	SLAY-screened peptide P5674			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCAGCTCTGACAATGCGCAGTGTTTCTACCACCATAATCATATCTCTTCTTCTTTCTCGTAA	PSSDNAQCFYHHNHISSSFS*	-1.04	0.005386	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27325	LCPSISDHMIAYSINPATFY			20	SLAY-screened peptide P5675			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTGCCCCTCCATTTCTGATCACATGATTGCTTATTCGATCAACCCTGCCACTTTTTACTAA	LCPSISDHMIAYSINPATFY*	-1.04	0.035621	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27326	LDPIYPGPVYLLLAYSRERR			20	SLAY-screened peptide P5676			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGACCCCATTTACCCGGGGCCCGTTTACTTGCTTCTCGCGTATTCTCGTGAGCGCCGCTAA	LDPIYPGPVYLLLAYSRERR*	-1.04	6.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27327	PELLGLPNSTEPYCFSLYRL			20	SLAY-screened peptide P5677			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGAGTTGCTTGGCCTTCCCAATAGCACTGAGCCCTATTGCTTTAGTCTGTATCGTCTCTAA	PELLGLPNSTEPYCFSLYRL*	-1.04	0.000176	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27328	APAVNFAPC			9	SLAY-screened peptide P5678			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCTGCCGTCAATTTTGCCCCTTGCTAAGGAGCCCTTGTCTCAACGGCTGTTCTTCCTAAC	APAVNFAPC*GALVSTAVLPN	-1.04	0.000376	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27329	YLPLLLPFTLIPSYHNCTDF			20	SLAY-screened peptide P5679			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTTGCCTCTGCTCCTCCCTTTCACTCTTATTCCCTCTTATCACAACTGCACGGATTTTTAA	YLPLLLPFTLIPSYHNCTDF*	-1.04	0.000425	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27330	DRVYASPNYYSQSSFQLYSL			20	SLAY-screened peptide P5680			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCGCGTTTATGCCAGTCCCAATTATTACAGCCAGTCGAGCTTTCAGCTGTACTCTTTGTAA	DRVYASPNYYSQSSFQLYSL*	-1.04	0.000397	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27331	APHDPDSLFPCLCYTNHRT			19	SLAY-screened peptide P5681			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCCTCATGACCCGGATTCGTTGTTCCCTTGCCTCTGTTACACTAACCACAGGACGTAGTAA	APHDPDSLFPCLCYTNHRT**	-1.039	0.005402	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27332	FPSTSGPVQWPFLQSRTPCL			20	SLAY-screened peptide P5682			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCCCTCTACTTCCGGGCCCGTCCAGTGGCCCTTCCTTCAGTCGCGGACCCCTTGTCTGTAA	FPSTSGPVQWPFLQSRTPCL*	-1.039	0.020039	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27333	NCRGALWPYIKFLYHNDNFI			20	SLAY-screened peptide P5683			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTGCCGCGGCGCTTTGTGGCCGTACATTAAGTTCCTTTATCACAATGATAACTTCATTTAA	NCRGALWPYIKFLYHNDNFI*	-1.039	0.034349	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27334	VTHNTFNICVTFYCRTINVS			20	SLAY-screened peptide P5684			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTACCCACAATACTTTTAATATTTGTGTCACGTTCTATTGTCGTACCATTAATGTGTCTTAA	VTHNTFNICVTFYCRTINVS*	-1.039	0.037793	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27335	RALMNIPDKSARCPSSPVFPN			21	SLAY-screened peptide P5685			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGCGTTGATGAACATCCCCGACAAGAGTGCACGGTGTCCGTCATCACCAGTATTTCCTAAC	RALMNIPDKSARCPSSPVFPN	-1.039	0.005691	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27336	VPVQLLG			7	SLAY-screened peptide P5686			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCCGGTGCAGCTTCTGGGCTAGTGTGAGTCTAAGGCCCGGCAGGACACCGAGAACTATTAA	VPVQLLG*CESKARQDTENY*	-1.039	0.005179	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27337	APGLRYTRPCCTARYIVYQV			20	SLAY-screened peptide P5687			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCCTGGTTTGCGCTATACTCGCCCTTGTTGCACCGCCCGCTACATTGTGTACCAGGTTTAA	APGLRYTRPCCTARYIVYQV*	-1.038	0.013367	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27338	TTTFSPFPYRISKILALAYT			20	SLAY-screened peptide P5688			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTACTACTTTTAGCCCGTTCCCCTACAGGATTTCTAAGATTCTGGCGCTTGCTTATACTTAA	TTTFSPFPYRISKILALAYT*	-1.038	0.024003	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27339	GTDSGFLPCHIPR			13	SLAY-screened peptide P5689			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGGACGGATAGCGGCTTCCTGCCTTGCCATATCCCGCGGTAGGTCACCTATAGCATGTTGTAA	GTDSGFLPCHIPR*VTYSML*	-1.037	1.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27340	AQPYHNLRCFPDNTHFDKSN			20	SLAY-screened peptide P5690			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCCAGCCTTATCACAATCTTCGTTGTTTTCCTGATAATACTCATTTCGACAAGTCTAATTAA	AQPYHNLRCFPDNTHFDKSN*	-1.037	0.00692	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27341	TNCPDLAYSIHNPLANLPGN			20	SLAY-screened peptide P5691			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCAATTGCCCGGATCTTGCTTACTCTATTCATAATCCCCTTGCGAATCTTCCGGGTAACTAA	TNCPDLAYSIHNPLANLPGN*	-1.037	0.001514	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27342	PFAATSASDQYAVNFQRYAH			20	SLAY-screened peptide P5692			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTTGCCGCCACTTCTGCTAGCGATCAGTATGCGGTCAACTTCCAGCGCTACGCGCACTAA	PFAATSASDQYAVNFQRYAH*	-1.037	0.003111	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27343	STAYSLLAPCDHIPRYIWTH			20	SLAY-screened peptide P5693			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTACCGCGTATAGTCTTCTCGCCCCCTGCGACCACATCCCCCGCTATATCTGGACTCATTAA	STAYSLLAPCDHIPRYIWTH*	-1.037	0.000723	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27344	CKTAMHPAGIVIICNEFSAM			20	SLAY-screened peptide P5694			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAAGACGGCGATGCATCCTGCGGGGATCGTTATTATCTGTAACGAGTTCTCTGCGATGTAA	CKTAMHPAGIVIICNEFSAM*	-1.037	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27345	DFPAFRWLNSLRLRLCTLSE			20	SLAY-screened peptide P5695			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACTTCCCCGCCTTCCGGTGGCTCAATTCCCTTCGCCTCCGGCTTTGCACGCTTTCCGAGTAA	DFPAFRWLNSLRLRLCTLSE*	-1.037	6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27346	PPCRTPYIPPRALNLELI			18	SLAY-screened peptide P5696			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCTGCCGCACCCCCTACATCCCTCCGCGTGCGCTTAATCTTGAGCTTATCTAGGTCTAA	PPCRTPYIPPRALNLELI*V*	-1.037	0.003049	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27347	KYACGYI			7	SLAY-screened peptide P5697			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGTACGCTTGTGGGTACATCTAGATGAATCATAACGCTAATCACCCCAATATTCGCTACTAA	KYACGYI*MNHNANHPNIRY*	-1.037	0.000132	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27348	RARHAPWRRRCICYRNPSPK			20	SLAY-screened peptide P5698			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCGCCCGGCACGCCCCCTGGCGGCGCCGCTGTATCTGCTATAGGAACCCGTCGCCCAAGTAA	RARHAPWRRRCICYRNPSPK*	-1.037	0.001386	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27349	ATSVPCSFPLYTASNYYITQ			20	SLAY-screened peptide P5699			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACTTCGGTGCCCTGTAGCTTTCCCTTGTATACGGCTTCGAATTATTACATTACGCAGTAA	ATSVPCSFPLYTASNYYITQ*	-1.036	0.005617	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27350	QHASSHHYRACR			12	SLAY-screened peptide P5700			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCATGCTTCCAGCCACCACTACAGGGCCTGCCGTTAGTTTTGCTCTCGGCTTACCGCTTAA	QHASSHHYRACR*FCSRLTA*	-1.036	0.000102	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27351	PPANRNSDLNTSNYCWCQYA			20	SLAY-screened peptide P5701			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCGCGAATCGTAACTCCGACCTTAACACCAGCAATTATTGTTGGTGTCAGTACGCTTAA	PPANRNSDLNTSNYCWCQYA*	-1.036	0.045244	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27352	ACPTLPWCPWITHAVGLYPI			20	SLAY-screened peptide P5702			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTGCCCGACCCTTCCGTGGTGCCCTTGGATCACCCACGCTGTCGGCTTGTACCCGATTTAA	ACPTLPWCPWITHAVGLYPI*	-1.036	0.011687	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27353	QPDRPSQTCTIRFPSSLVRL			20	SLAY-screened peptide P5703			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGCCGGACCGGCCTTCTCAGACTTGCACTATCAGGTTCCCGTCCTCTCTTGTCAGGCTTTAA	QPDRPSQTCTIRFPSSLVRL*	-1.036	0.014967	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27354	PHNGIDEFSKYDRYGDIHCL			20	SLAY-screened peptide P5704			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCACAACGGTATCGATGAGTTTAGCAAGTACGACAGGTACGGTGATATTCATTGCCTTTAA	PHNGIDEFSKYDRYGDIHCL*	-1.036	0.008246	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27355	PCTMAGRARHLELDQLPQQFV			21	SLAY-screened peptide P5705			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCTGCACGATGGCGGGTCGCGCCCGGCACTTGGAATTGGACCAACTGCCCCAGCAATTCGTC	PCTMAGRARHLELDQLPQQFV	-1.035	0.000144	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27356	DHRGTLLSDVKLGPEDRNPT			20	SLAY-screened peptide P5706			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCATAGGGGTACGCTTCTGAGCGATGTCAAGTTGGGGCCGGAGGATCGCAATCCTACGTAA	DHRGTLLSDVKLGPEDRNPT*	-1.035	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27357	RIITYWSALKNHFRTGSYPR			20	SLAY-screened peptide P5707			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATCATCACGTACTGGTCTGCGCTGAAGAACCACTTCAGGACGGGGAGTTATCCCAGGTAA	RIITYWSALKNHFRTGSYPR*	-1.035	0.005239	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27358	AGRYSSGRYHSRGGESVR			18	SLAY-screened peptide P5708			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGGCCGCTATTCTAGTGGCCGGTATCATTCTCGCGGCGGTGAGTCGGTGCGTTAGTTCTAA	AGRYSSGRYHSRGGESVR*F*	-1.035	0.007312	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27359	AISCDRIPFRRRTPIPQLRF			20	SLAY-screened peptide P5709			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGATTTCGTGTGATCGTATTCCCTTTCGGCGTCGCACTCCTATTCCCCAGCTTAGGTTCTAA	AISCDRIPFRRRTPIPQLRF*	-1.035	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27360	SAPSKYLRTLYNLVCDRAYT			20	SLAY-screened peptide P5710			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTGCTCCCTCCAAGTACCTGCGTACGTTGTATAATCTTGTTTGTGATAGGGCGTATACCTAA	SAPSKYLRTLYNLVCDRAYT*	-1.035	0.015532	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27361	MHSAHAPLFNCRHHLYDWSY			20	SLAY-screened peptide P5711			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCATAGTGCCCACGCCCCTCTCTTTAATTGCCGGCACCATTTGTACGACTGGAGCTACTAA	MHSAHAPLFNCRHHLYDWSY*	-1.035	0.001152	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27362	IYT			3	SLAY-screened peptide P5712			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCTACACCTAGGCGAGCCCCGTCTAGCTGAGTCACTGCCGGGTTACGAGCAAGGACACCTAA	IYT*ASPV*LSHCRVTSKDT*	-1.034	0.000912	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27363	SCTYYISRPFTFQPISNRRA			20	SLAY-screened peptide P5713			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTTGTACGTATTACATCAGTAGGCCTTTCACCTTTCAGCCGATTTCGAATCGTAGGGCTTAA	SCTYYISRPFTFQPISNRRA*	-1.034	0.001588	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27364	PTKLLPAIFNCDQNAFL			17	SLAY-screened peptide P5714			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCACGAAGCTTCTCCCTGCGATTTTTAATTGTGACCAGAACGCGTTTCTCTAGACGATTTAA	PTKLLPAIFNCDQNAFL*TI*	-1.034	2.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27365	PIASTCLPERASSSTMRTSG			20	SLAY-screened peptide P5715			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATCGCCTCGACGTGTTTGCCGGAGCGCGCTTCCTCCTCCACTATGAGGACCTCCGGTTAA	PIASTCLPERASSSTMRTSG*	-1.034	0.003207	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27366	PPYPFTASTGSS			12	SLAY-screened peptide P5716			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCTTACCCCTTTACCGCCAGTACGGGTTCCTCTTAGACCCATAAGCTCACTTCCAATTAA	PPYPFTASTGSS*THKLTSN*	-1.034	0.037974	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27367	LPLFSGTCMGPSPYYFNSLL			20	SLAY-screened peptide P5717			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCGCTGTTTTCCGGCACGTGCATGGGTCCGAGCCCGTACTACTTTAACAGTCTTCTGTAA	LPLFSGTCMGPSPYYFNSLL*	-1.033	0.007755	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27368	MYLYDNNFHYNCIRLSHSRA			20	SLAY-screened peptide P5718			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTATCTTTACGACAACAACTTTCATTATAACTGTATTCGCCTCTCTCATAGTCGTGCGTAA	MYLYDNNFHYNCIRLSHSRA*	-1.033	0.019663	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27369	PICAPYDTNANTVPYCILLF			20	SLAY-screened peptide P5719			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCATTTGCGCGCCGTACGACACTAATGCTAACACTGTCCCTTACTGTATTCTGCTGTTTTAA	PICAPYDTNANTVPYCILLF*	-1.033	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27370	TYPKPAYDHCEIFHDYHTHF			20	SLAY-screened peptide P5720			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTATCCTAAGCCCGCTTATGATCACTGTGAGATCTTTCACGACTACCACACTCATTTTTAA	TYPKPAYDHCEIFHDYHTHF*	-1.033	0.02322	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27371	PPVSQRAAATPNFFTAISSH			20	SLAY-screened peptide P5721			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCGGTTAGTCAGCGCGCGGCCGCCACCCCTAACTTTTTTACGGCTATTAGCTCCCATTAA	PPVSQRAAATPNFFTAISSH*	-1.033	4.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27372	LSHSFIFQVVHRPKTCYNNK			20	SLAY-screened peptide P5722			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCGCACAGTTTCATCTTCCAGGTTGTCCACCGTCCTAAGACGTGTTACAACAACAAGTAA	LSHSFIFQVVHRPKTCYNNK*	-1.033	0.00097	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27373	FLTVAPSWHLLVRPAVGLVSN			21	SLAY-screened peptide P5723			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCTTACCGTTGCGCCTTCGTGGCATTTATTAGTTAGACCCGCAGTAGGACTAGTTTCTAAC	FLTVAPSWHLLVRPAVGLVSN	-1.032	0.0023	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27374	DPDTSPFCRSVHTLDTSKPT			20	SLAY-screened peptide P5724			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACCCGGATACTTCTCCCTTTTGTCGGTCCGTTCATACCCTCGATACCTCTAAGCCTACGTAA	DPDTSPFCRSVHTLDTSKPT*	-1.032	0.039011	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27375	QIKCTCPSYGYWLWPHTMTH			20	SLAY-screened peptide P5725			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGATTAAGTGCACCTGCCCGAGCTATGGCTACTGGCTTTGGCCCCACACGATGACTCATTAA	QIKCTCPSYGYWLWPHTMTH*	-1.031	6.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27376	SKPCSRAFYPLYQAI			15	SLAY-screened peptide P5726			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGAAGCCTTGCTCGCGGGCCTTCTACCCTCTCTACCAGGCCATTTAGTTCCCCATCGCTTAA	SKPCSRAFYPLYQAI*FPIA*	-1.031	0.038429	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27377	VNAANPTGISYRPPLSST			18	SLAY-screened peptide P5727			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTAATGCGGCCAATCCGACTGGGATCTCCTATCGGCCTCCTCTTTCCTCTACCTAGATGTAA	VNAANPTGISYRPPLSST*M*	-1.031	0.001955	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27378	HIAASVGYFKFDYYNGITLQ			20	SLAY-screened peptide P5728			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACATCGCCGCCTCTGTTGGCTACTTTAAGTTTGATTATTATAATGGTATCACGCTCCAGTAA	HIAASVGYFKFDYYNGITLQ*	-1.031	0.034781	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27379	CLSLMCESPRYFCNWASKPP			20	SLAY-screened peptide P5729			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCTCTCTCTCATGTGCGAGAGCCCCCGTTACTTCTGTAACTGGGCCTCCAAGCCGCCTTAA	CLSLMCESPRYFCNWASKPP*	-1.031	0.036867	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27380	IILMLPSSYQEDARSCPTTR			20	SLAY-screened peptide P5730			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCATCCTTATGCTCCCTAGTAGTTACCAGGAGGATGCTAGGTCCTGTCCGACTACCAGGTAA	IILMLPSSYQEDARSCPTTR*	-1.03	0.009093	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27381	PLRFLAPLPIPMVSRRPRSRN			21	SLAY-screened peptide P5731			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCTGAGATTTTTAGCCCCACTCCCAATTCCCATGGTCAGTAGGCGTCCCCGGTCTCGTAAC	PLRFLAPLPIPMVSRRPRSRN	-1.03	0.006546	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27382	VPRTMYYVNTLHASNIKRRD			20	SLAY-screened peptide P5732			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCCTAGGACTATGTATTACGTCAACACTTTGCACGCTAGTAATATTAAGCGGCGTGATTAA	VPRTMYYVNTLHASNIKRRD*	-1.03	8.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27383	SHQAYVTLIDSRD			13	SLAY-screened peptide P5733			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCTCACCAGGCGTACGTGACCCTTATTGACTCCCGCGACTAGATTAAGCCCGATTTTACCTAA	SHQAYVTLIDSRD*IKPDFT*	-1.03	0.021032	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27384	YWFKRWNCMVISLI			14	SLAY-screened peptide P5734			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTGGTTCAAGCGGTGGAATTGTATGGTTATTAGCCTCATTTAGCCCTTCTGGGACCCCTAA	YWFKRWNCMVISLI*PFWDP*	-1.029	0.027573	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27385	PLTYAPFVVINSPWSCLHTY			20	SLAY-screened peptide P5735			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTGACCTATGCTCCTTTTGTGGTCATTAACTCGCCTTGGTCGTGTTTGCATACCTATTAA	PLTYAPFVVINSPWSCLHTY*	-1.028	0.039129	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27386	PGPCCCWDPLYRIPIANIA			19	SLAY-screened peptide P5736			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGGGCCTTGCTGTTGCTGGGACCCGCTTTACAGGATTCCTATTGCCAATATCGCCTAGTAA	PGPCCCWDPLYRIPIANIA**	-1.028	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27387	VCKLSDGVSDRYGRILLGYL			20	SLAY-screened peptide P5737			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTCTGCAAGCTTTCTGATGGTGTTTCCGACCGTTACGGCCGTATCCTGCTTGGCTACCTTTAA	VCKLSDGVSDRYGRILLGYL*	-1.028	0.012418	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27388	FLLFYLLRALITSCKSHLRL			20	SLAY-screened peptide P5738			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCTGTTGTTCTACCTGTTGAGGGCGCTCATCACGAGTTGTAAGTCCCACTTGCGCTTGTAA	FLLFYLLRALITSCKSHLRL*	-1.028	0.000129	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27389	VCAKYYPLFLFDNFQWGDKI			20	SLAY-screened peptide P5739			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTGCGCGAAGTACTATCCCCTTTTTCTCTTCGACAATTTCCAGTGGGGCGACAAGATTTAA	VCAKYYPLFLFDNFQWGDKI*	-1.028	0.005458	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27390	SHCNDAHVSPVNILHTCIIY			20	SLAY-screened peptide P5740			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCACTGCAACGACGCTCACGTGTCGCCCGTTAACATCCTGCATACCTGCATTATCTACTAA	SHCNDAHVSPVNILHTCIIY*	-1.028	0.004815	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27391	MTCLICLPFLLVSPSITRLGN			21	SLAY-screened peptide P5741			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGACCTGTCTAATTTGTCTACCCTTCCTCCTTGTGTCACCATCCATAACACGTTTGGGTAAC	MTCLICLPFLLVSPSITRLGN	-1.028	0.000745	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27392	EDNCLKRLYIPLHIVHFYT			19	SLAY-screened peptide P5742			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGACAATTGCTTGAAGCGCCTTTACATCCCTCTTCACATTGTTCACTTCTACACTTAGTAA	EDNCLKRLYIPLHIVHFYT**	-1.028	3.7e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27393	AAMDYVCEANLKVSIVTSC			19	SLAY-screened peptide P5743			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCGCTATGGACTATGTTTGTGAGGCCAACCTCAAGGTTAGCATTGTGACCAGTTGTTAGTAA	AAMDYVCEANLKVSIVTSC**	-1.028	0.029881	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27394	CSTDFTLWHPLTIHQTNLAM			20	SLAY-screened peptide P5744			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAGCACCGATTTTACGCTTTGGCACCCTCTTACTATTCACCAGACTAATTTGGCCATGTAA	CSTDFTLWHPLTIHQTNLAM*	-1.027	3.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27395	HSGPSSMGSFFSDFVHSRAP			20	SLAY-screened peptide P5745			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTCGGGTCCTTCGTCCATGGGCAGCTTTTTTAGTGATTTTGTGCACTCTCGCGCGCCCTAA	HSGPSSMGSFFSDFVHSRAP*	-1.027	0.038056	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27396	VGGPCGILVA			10	SLAY-screened peptide P5746			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGGTGGCCCGTGTGGGATACTCGTGGCATGATCATTAATTACCGCTCGTGTAAGCCGTAAC	VGGPCGILVA*SLITARVSRN	-1.027	0.001379	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27397	CHYNIGSDDTCHRLYEFKLL			20	SLAY-screened peptide P5747			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCATTATAATATCGGTTCTGACGATACTTGTCACCGTTTGTACGAGTTTAAGCTGCTGTAA	CHYNIGSDDTCHRLYEFKLL*	-1.027	0.004147	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27398	IDAEAHQNCFLMGMCHPCIL			20	SLAY-screened peptide P5748			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGACGCTGAGGCGCACCAGAATTGTTTTCTGATGGGCATGTGCCACCCGTGTATCCTTTAA	IDAEAHQNCFLMGMCHPCIL*	-1.026	9.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27399	GSASPLPAGPFHAPPFNFSS			20	SLAY-screened peptide P5749			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTCCGCCAGTCCTCTTCCTGCTGGCCCGTTCCATGCGCCCCCCTTTAATTTCTCTTCGTAA	GSASPLPAGPFHAPPFNFSS*	-1.026	0.028968	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27400	RVGETLTNFYNLLVSGCSQK			20	SLAY-screened peptide P5750			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGTCGGGGAGACCCTCACGAATTTCTATAATCTCCTGGTTTCCGGGTGCTCTCAGAAGTAA	RVGETLTNFYNLLVSGCSQK*	-1.026	0.000728	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27401	TALPRWYRMLRDMLLQTIPL			20	SLAY-screened peptide P5751			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGCGCTTCCGCGGTGGTACCGCATGCTGCGCGATATGCTCTTGCAGACCATTCCGCTGTAA	TALPRWYRMLRDMLLQTIPL*	-1.026	0.011123	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27402	HVGTAQHHPLSHTRDSNDTK			20	SLAY-screened peptide P5752			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGTTGGCACCGCGCAGCACCATCCGCTTTCTCACACTCGCGACAGCAACGACACTAAGTAA	HVGTAQHHPLSHTRDSNDTK*	-1.026	0.009804	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27403	CPIRAPSFVTLMTWDGGRLA			20	SLAY-screened peptide P5753			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCCTATCCGCGCCCCGTCGTTTGTTACGCTTATGACGTGGGACGGTGGCCGCCTCGCTTAA	CPIRAPSFVTLMTWDGGRLA*	-1.026	0.005458	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27404	PISPVFQRFYHTSPWPLNHS			20	SLAY-screened peptide P5754			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTATTTCGCCTGTGTTCCAGCGTTTTTACCACACTTCTCCGTGGCCGCTCAACCACTCCTAA	PISPVFQRFYHTSPWPLNHS*	-1.026	0.000274	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27405	PLIVNTAHGDNIHIIPSVLL			20	SLAY-screened peptide P5755			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTGATTGTTAATACTGCCCATGGGGATAACATCCATATTATCCCCAGTGTGTTGCTCTAA	PLIVNTAHGDNIHIIPSVLL*	-1.025	0.005153	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27406	TTYIYHLVLNSDLDMRCRNA			20	SLAY-screened peptide P5756			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCACCTACATCTATCACTTGGTCCTGAATTCGGACCTCGATATGCGTTGTCGCAATGCTTAA	TTYIYHLVLNSDLDMRCRNA*	-1.025	0.001867	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27407	LG			2	SLAY-screened peptide P5757			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCGGCTAGCCCTCCTCTCGCTTCGATTCCAACGAGGCGTACAGGGCCCATTTCCAGGCCTAA	LG*PSSRFDSNEAYRAHFQA*	-1.025	0.016345	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27408	IHV			3	SLAY-screened peptide P5758			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCCATGTCTAGCTTGTTTGCGATACGTACAGCGCCGCGCCCCATCGTACTCTTACTAACTGA	IHV*LVCDTYSAAPHRTLTN*	-1.025	0.026433	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27409	RCAIPHFSPSLPPWWCCYAT			20	SLAY-screened peptide P5759			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTTGCGCCATCCCCCATTTTTCTCCCTCTCTGCCGCCTTGGTGGTGCTGCTATGCTACCTAA	RCAIPHFSPSLPPWWCCYAT*	-1.025	0.034218	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27410	IALVRVSASQLTPFACAIAL			20	SLAY-screened peptide P5760			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGCCCTCGTGCGTGTCTCTGCTTCCCAGCTCACCCCGTTTGCTTGCGCCATCGCCCTCTAA	IALVRVSASQLTPFACAIAL*	-1.025	0.025487	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27411	LLAVNMSDPLTVLTYQSRPP			20	SLAY-screened peptide P5761			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCTCGCTGTTAACATGTCTGACCCCCTGACTGTTCTGACGTATCAGTCTCGGCCCCCCTAA	LLAVNMSDPLTVLTYQSRPP*	-1.025	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27412	PPALKRPKEYLVIEGACLAG			20	SLAY-screened peptide P5762			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCGGCGTTGAAGAGGCCGAAGGAGTACCTGGTTATTGAGGGGGCTTGCCTCGCTGGTTAA	PPALKRPKEYLVIEGACLAG*	-1.024	0.000689	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27413	PHLHAPTWVPCYSYDLAPHN			20	SLAY-screened peptide P5763			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCACCTGCACGCTCCTACGTGGGTCCCGTGCTATTCCTATGATCTTGCTCCCCACAATTAA	PHLHAPTWVPCYSYDLAPHN*	-1.024	6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27414	TAICTFAPHSIDRNKNARLHL			21	SLAY-screened peptide P5764			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTGCGATTTGTACCTTCGCTCCGCATTCCATTGACCGCAACAAGAATGCACGACTCCACTTA	TAICTFAPHSIDRNKNARLHL	-1.023	0.045148	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27415	YSPLRIYICKSLHPFMDPAS			20	SLAY-screened peptide P5765			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTCCCCTCTCCGCATCTATATTTGTAAGTCCCTTCACCCGTTTATGGACCCGGCGTCGTAA	YSPLRIYICKSLHPFMDPAS*	-1.023	0.000149	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27416	TATVTMPDKCSFSMTPGGISN			21	SLAY-screened peptide P5766			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGCTACCGTCACCATGCCCGATAAGTGTTCCTTTAGTATGACCCCGGGGGGTATAAGTAAC	TATVTMPDKCSFSMTPGGISN	-1.023	0.007474	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27417	RLVIHPSPSESIPKTRGLPP			20	SLAY-screened peptide P5767			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCTCGTGATTCATCCGAGCCCGAGCGAGAGCATTCCCAAGACCAGGGGTCTCCCTCCTTAA	RLVIHPSPSESIPKTRGLPP*	-1.023	0.003898	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27418	LFHNDLESTDPAICLRRYYG			20	SLAY-screened peptide P5768			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTTTCCATAATGACCTTGAGAGCACCGACCCGGCGATTTGTCTTCGCCGCTATTATGGTTAA	LFHNDLESTDPAICLRRYYG*	-1.023	0.042572	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27419	LPSAAKNVARQSTSSHYRCFV			21	SLAY-screened peptide P5769			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCCCCTCTGCGGCGAAGAATGTTGCCCGGCAGAGTACTTCCTCTCACTACCGCTGCTTTGTA	LPSAAKNVARQSTSSHYRCFV	-1.022	0.001905	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27420	LCHQFSNVLCTQLTMRYIMD			20	SLAY-screened peptide P5770			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTGTCACCAGTTTAGTAATGTCTTGTGCACTCAGCTTACTATGCGGTATATCATGGATTAA	LCHQFSNVLCTQLTMRYIMD*	-1.022	0.021789	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27421	SSPHRSCSSLPVILASNPTA			20	SLAY-screened peptide P5771			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTTCCCCCCATCGGTCGTGCTCCTCGCTGCCGGTCATCTTGGCCTCGAACCCGACCGCGTAA	SSPHRSCSSLPVILASNPTA*	-1.022	0.000276	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27422	WLTAGSNHLPFLYWLGGCYR			20	SLAY-screened peptide P5772			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCTCACTGCCGGTTCTAATCATCTGCCGTTCTTGTACTGGCTGGGCGGCTGCTACCGTTAA	WLTAGSNHLPFLYWLGGCYR*	-1.022	0.000307	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27423	CLWHYYSLFANVDDGIINSF			20	SLAY-screened peptide P5773			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTTGTGGCACTATTACTCCCTGTTCGCCAATGTCGATGATGGCATTATTAACAGTTTCTAA	CLWHYYSLFANVDDGIINSF*	-1.022	0.021297	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27424	GKWCTPLPALFVIDFKEMTK			20	SLAY-screened peptide P5774			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGCAAGTGGTGCACGCCGCTGCCCGCGTTGTTTGTGATTGATTTTAAGGAGATGACTAAGTAA	GKWCTPLPALFVIDFKEMTK*	-1.022	0.013173	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27425	CPRILSPYWLYSTLTSNAHK			20	SLAY-screened peptide P5775			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTCCCCGGATTCTTAGCCCTTATTGGCTCTACAGTACTCTCACGTCGAATGCGCACAAGTAA	CPRILSPYWLYSTLTSNAHK*	-1.022	0.045395	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27426	FSHNYARFKVCLMLPNLLSD			20	SLAY-screened peptide P5776			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCAGTCATAATTATGCCAGGTTTAAGGTCTGCCTTATGCTGCCCAACCTTCTGTCGGATTAA	FSHNYARFKVCLMLPNLLSD*	-1.022	0.004529	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27427	AFPIMPTYQRSFFTSYFYAI			20	SLAY-screened peptide P5777			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGTTCCCTATTATGCCTACGTATCAGCGCTCTTTTTTTACCTCTTATTTTTATGCTATTTAA	AFPIMPTYQRSFFTSYFYAI*	-1.021	0.006572	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27428	FAYALL			6	SLAY-screened peptide P5778			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCGCCTACGCCCTGCTTTAGAACCATCTGAAGAAGATTGTTTTTGGCTCTCACTCTTCCTAA	FAYALL*NHLKKIVFGSHSS*	-1.021	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27429	APALLTLSLSTVLLIIVWCLN			21	SLAY-screened peptide P5779			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCCCGCCTTACTTACACTTAGCCTATCGACCGTCCTCTTGATTATCGTGTGGTGTCTTAAC	APALLTLSLSTVLLIIVWCLN	-1.021	0.010422	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27430	HCLNGLRKTILYPTSLVTDL			20	SLAY-screened peptide P5780			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTGTCTTAACGGTTTGCGGAAGACGATTCTGTATCCCACTAGCCTTGTCACCGATTTGTAA	HCLNGLRKTILYPTSLVTDL*	-1.021	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27431	GRCTDPSSCIKNLVKCLWTK			20	SLAY-screened peptide P5781			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTAGGTGCACCGATCCTAGCTCGTGCATCAAGAATCTCGTTAAGTGTCTCTGGACGAAGTAA	GRCTDPSSCIKNLVKCLWTK*	-1.021	0.00089	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27432	FGGYRDYDHSQQSKRT			16	SLAY-screened peptide P5782			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTGGTGGTTATCGTGACTACGACCATTCTCAGCAGAGTAAGCGCACTTGACGCGCTTGTAAC	FGGYRDYDHSQQSKRT*RACN	-1.021	0.001194	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27433	RKFRRLYMSYPQYCPHYDDY			20	SLAY-screened peptide P5783			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAAGTTTCGGAGGCTGTACATGAGCTATCCCCAGTATTGCCCGCATTATGACGACTACTAA	RKFRRLYMSYPQYCPHYDDY*	-1.02	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27434	EHFEKTSNSPCFILSWPSIA			20	SLAY-screened peptide P5784			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGCACTTTGAGAAGACCTCCAACTCCCCCTGTTTCATTCTCAGTTGGCCGTCCATCGCGTAA	EHFEKTSNSPCFILSWPSIA*	-1.02	0.001016	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27435	TTPGCYLALFLRTLSNSHYN			20	SLAY-screened peptide P5785			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTACGCCCGGCTGTTATCTTGCCCTTTTTCTTCGCACGCTGAGTAATTCGCATTACAACTAA	TTPGCYLALFLRTLSNSHYN*	-1.02	0.008648	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27436	LTPHLSGDNSHAVTSKTWSI			20	SLAY-screened peptide P5786			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTACTCCGCATCTTAGCGGGGATAACAGCCACGCTGTTACGAGTAAGACCTGGAGTATTTAA	LTPHLSGDNSHAVTSKTWSI*	-1.02	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27437	PAPFIFRHWCDNTLY			15	SLAY-screened peptide P5787			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTGCGCCGTTCATCTTTCGTCACTGGTGCGATAATACCCTTTACTAGGCCGCTCTTACCTAA	PAPFIFRHWCDNTLY*AALT*	-1.02	0.001245	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27438	LEILPARNNVAPCYLLLVRT			20	SLAY-screened peptide P5788			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGAGATTCTGCCCGCTCGCAACAACGTCGCCCCTTGTTACCTCTTGCTCGTCCGTACTTAA	LEILPARNNVAPCYLLLVRT*	-1.02	0.00777	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27439	SNNSSHPLLGRRIYSYFSLG			20	SLAY-screened peptide P5789			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCAATAACTCCTCCCATCCTCTGCTCGGGCGCCGGATTTATTCCTATTTTTCCCTTGGTTAA	SNNSSHPLLGRRIYSYFSLG*	-1.02	0.009087	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27440	TPGGEHLHRPRGPPIRNEKM			20	SLAY-screened peptide P5790			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCCGGGTGGTGAGCATCTGCACAGGCCCCGGGGCCCCCCCATCCGTAACGAGAAGATGTAA	TPGGEHLHRPRGPPIRNEKM*	-1.019	1e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27441	PPLYNATAGRILPEALVRFV			20	SLAY-screened peptide P5791			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCCCCTCTACAACGCGACCGCCGGCCGTATTTTGCCTGAGGCCCTTGTTCGCTTCGTCTAA	PPLYNATAGRILPEALVRFV*	-1.019	0.002633	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27442	PGARAKDWCLYPTYPPNIAS			20	SLAY-screened peptide P5792			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGGTGCCCGCGCCAAGGACTGGTGCTTGTACCCCACCTACCCCCCGAACATCGCTAGTTAA	PGARAKDWCLYPTYPPNIAS*	-1.019	0.005727	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27443	VGRVPFSCKSIWPQLWVALFN			21	SLAY-screened peptide P5793			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTGGCCGCGTTCCTTTCTCTTGTAAGTCGATCTGGCCGCAGCTCTGGGTCGCACTCTTTAAC	VGRVPFSCKSIWPQLWVALFN	-1.018	0.01752	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27444	NSSNKDGYNHSRSYICHSYN			20	SLAY-screened peptide P5794			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAACTCCTCTAATAAGGATGGCTACAACCATAGCCGTTCCTACATCTGTCACAGCTATAACTAA	NSSNKDGYNHSRSYICHSYN*	-1.018	0.048354	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27445	TNPASYCSPVGWRLIRNVDA			20	SLAY-screened peptide P5795			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTAACCCGGCTAGTTATTGTTCTCCCGTCGGGTGGCGCCTGATCCGTAATGTTGATGCTTAA	TNPASYCSPVGWRLIRNVDA*	-1.018	0.000708	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27446	SLSILRDELVTRVT			14	SLAY-screened peptide P5796			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCCTTAGTATCCTTCGCGATGAATTGGTAACACGTGTGACCTGAGTACTATGGTTGGTTAAC	SLSILRDELVTRVT*VLWLVN	-1.018	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27447	TVQNNNTGLR			10	SLAY-screened peptide P5797			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCGTCCAGAATAACAATACTGGGCTGCGTTAGCTGAGCCGCTATTACAACCATAATCTCTAA	TVQNNNTGLR*LSRYYNHNL*	-1.018	0.004329	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27448	RIHTYATSLFPMHILTGFDT			20	SLAY-screened peptide P5798			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCATTCATACCTACGCTACTTCGTTGTTCCCGATGCATATCCTGACGGGTTTTGATACGTAA	RIHTYATSLFPMHILTGFDT*	-1.018	5e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27449	HTPLLPYVDYAHYFYERSPN			20	SLAY-screened peptide P5799			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACACGCCGCTGCTTCCCTATGTCGATTACGCTCACTATTTTTACGAGCGTAGTCCGAATTAA	HTPLLPYVDYAHYFYERSPN*	-1.017	0.001858	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27450	ATRSSGHLDLPFGSNVMLDL			20	SLAY-screened peptide P5800			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCACCAGGTCCAGCGGTCACCTCGATCTCCCCTTTGGCTCCAACGTTATGCTGGACCTTTAA	ATRSSGHLDLPFGSNVMLDL*	-1.017	0.007259	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27451	RGKRDLMQIRKKILTKMCDN			20	SLAY-screened peptide P5801			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTGGCAAGCGCGATCTGATGCAGATCCGGAAGAAGATTCTTACTAAGATGTGTGATAACTAA	RGKRDLMQIRKKILTKMCDN*	-1.017	0.042825	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27452	TQVCCNLVYCQYPDHYLHRL			20	SLAY-screened peptide P5802			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCAGGTCTGTTGTAATCTGGTTTATTGTCAGTACCCTGATCATTACCTGCATCGGCTGTAA	TQVCCNLVYCQYPDHYLHRL*	-1.017	0.005816	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27453	NLRPDAARDFCTCHFML			17	SLAY-screened peptide P5803			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTTGCGCCCGGATGCTGCGCGCGATTTCTGTACGTGCCACTTCATGCTCTAGCTCGCGTAA	NLRPDAARDFCTCHFML*LA*	-1.016	0.040802	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27454	LCGSTAHVRKYSTNMPIIHL			20	SLAY-screened peptide P5804			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTGCGGGAGTACTGCGCATGTGAGGAAGTACTCTACGAATATGCCCATTATTCATTTGTAA	LCGSTAHVRKYSTNMPIIHL*	-1.016	0.006575	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27455	RFPTDHSPCISFQLVKLLYI			20	SLAY-screened peptide P5805			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTTTCCTACCGACCATTCGCCTTGTATCTCCTTTCAGCTCGTTAAGCTTCTTTATATCTAA	RFPTDHSPCISFQLVKLLYI*	-1.016	0.007626	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27456	ADTLPGCTMTPCHAWLSYCI			20	SLAY-screened peptide P5806			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGGACACCCTCCCCGGCTGTACTATGACGCCCTGCCACGCCTGGCTCTCTTATTGTATTTAA	ADTLPGCTMTPCHAWLSYCI*	-1.016	0.007835	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27457	AYHRPCNESTYYSFPVSYVC			20	SLAY-screened peptide P5807			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTACCACCGGCCTTGCAACGAGTCGACGTATTACTCCTTCCCTGTCTCTTACGTTTGTTAA	AYHRPCNESTYYSFPVSYVC*	-1.016	0.007627	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27458	HLHIRWTARKGHTTSNRTRP			20	SLAY-screened peptide P5808			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATCTCCACATCCGGTGGACTGCCCGTAAGGGCCACACCACGTCGAATAGGACTCGCCCGTAA	HLHIRWTARKGHTTSNRTRP*	-1.016	0.000307	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27459	DPNHLARLLRWAKIRFLDRP			20	SLAY-screened peptide P5809			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATCCTAATCACCTCGCGCGTCTTCTGAGGTGGGCGAAGATTCGGTTCCTTGATAGGCCCTAA	DPNHLARLLRWAKIRFLDRP*	-1.016	0.005153	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27460	PHAKGSSDHHKTPNPSAAKR			20	SLAY-screened peptide P5810			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCATGCTAAGGGCTCCTCTGACCATCATAAGACGCCCAACCCCTCTGCTGCTAAGCGCTAA	PHAKGSSDHHKTPNPSAAKR*	-1.015	0.006701	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27461	IAHPLCPSRSSVAGFSASALT			21	SLAY-screened peptide P5811			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATTGCGCATCCACTTTGTCCATCTAGAAGCTCCGTCGCGGGTTTCAGCGCCTCCGCGCTAACT	IAHPLCPSRSSVAGFSASALT	-1.015	0.000456	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27462	VCCAALSCFYRLISATTTNTN			21	SLAY-screened peptide P5812			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTTGTTGCGCTGCCTTGTCGTGTTTTTACCGGCTGATTAGTGCTACGACTACCAACACTAAC	VCCAALSCFYRLISATTTNTN	-1.015	0.007101	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27463	HVLIFTLLRLSRRPILPN			18	SLAY-screened peptide P5813			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGTGCTCATTTTTACTTTACTAAGATTAAGTCGGCGGCCAATACTACCTAACTGAGTAAGT	HVLIFTLLRLSRRPILPN*VS	-1.015	0.005859	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27464	VQTSIFHLVTEHHLSIRFSD			20	SLAY-screened peptide P5814			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTGCAGACGAGCATTTTCCATTTGGTCACTGAGCATCATTTGTCTATCAGGTTCAGTGATTAA	VQTSIFHLVTEHHLSIRFSD*	-1.015	7e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27465	CARASPCPCDFFWSQDYFLT			20	SLAY-screened peptide P5815			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCGCCCGGGCTAGCCCCTGTCCGTGCGACTTTTTCTGGAGCCAGGATTACTTCCTGACCTAA	CARASPCPCDFFWSQDYFLT*	-1.015	0.041383	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27466	RYRLPIYFVSANRCVQSLLR			20	SLAY-screened peptide P5816			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTACCGCCTTCCGATCTATTTTGTCTCGGCGAATCGCTGCGTTCAGTCTCTTCTTCGGTAA	RYRLPIYFVSANRCVQSLLR*	-1.015	0.005666	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27467	GGPHNRLIPTDPSYLIIMLK			20	SLAY-screened peptide P5817			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTGGCCCTCACAACCGCCTTATTCCTACGGACCCGTCCTATTTGATCATTATGCTTAAGTAA	GGPHNRLIPTDPSYLIIMLK*	-1.015	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27468	DVTRTIYNSVTICKNPALPS			20	SLAY-screened peptide P5818			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATGTCACTCGTACGATTTATAATAGCGTCACCATTTGCAAGAACCCTGCGTTGCCCTCCTAA	DVTRTIYNSVTICKNPALPS*	-1.014	0.013553	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27469	CSNYPDRNLMSDMNLRQNSP			20	SLAY-screened peptide P5819			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAGTAACTATCCCGATCGTAATCTGATGTCGGATATGAACCTCCGCCAGAATAGTCCCTAA	CSNYPDRNLMSDMNLRQNSP*	-1.014	1.6e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27470	SWLCPNLPHADWRLNQMLIG			20	SLAY-screened peptide P5820			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGTGGCTTTGTCCCAACCTCCCCCACGCCGACTGGCGCCTCAATCAGATGCTGATTGGGTAA	SWLCPNLPHADWRLNQMLIG*	-1.014	0.007521	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27471	LPGKQPQPHHDNL			13	SLAY-screened peptide P5821			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTCCCGGTAAGCAGCCCCAGCCCCATCATGACAACCTCTAGTACTCCAAGCGGAACCGTTAA	LPGKQPQPHHDNL*YSKRNR*	-1.014	0.016037	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27472	TPYCAGLSRHRSRIFNYYT			19	SLAY-screened peptide P5822			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCCGTATTGTGCTGGCCTTTCCCGCCATAGGAGCCGCATTTTTAATTATTACACCTAGTAA	TPYCAGLSRHRSRIFNYYT**	-1.013	0.00958	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27473	YTNDYSCPNQAHPWNKDYII			20	SLAY-screened peptide P5823			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACACCAATGATTATAGCTGTCCGAACCAGGCCCATCCCTGGAATAAGGATTACATCATTTAA	YTNDYSCPNQAHPWNKDYII*	-1.013	0.029342	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27474	KHAESMLRSR			10	SLAY-screened peptide P5824			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCATGCCGAGTCCATGCTTAGGTCCAGGTGATCACGGACTCTGTTTCCACCCAGTTGTAAC	KHAESMLRSR*SRTLFPPSCN	-1.013	0.004393	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27475	AFTGGHPCFIHIISHEPYLL			20	SLAY-screened peptide P5825			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCCTTCACTGGTGGTCACCCCTGCTTTATCCACATTATTAGTCACGAGCCGTATTTGCTCTAA	AFTGGHPCFIHIISHEPYLL*	-1.013	0.000132	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27476	HTPFRRPFNSLCCVDKCSYT			20	SLAY-screened peptide P5826			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATACGCCCTTTCGTAGGCCCTTCAACTCTCTTTGTTGCGTCGACAAGTGTAGTTACACTTAA	HTPFRRPFNSLCCVDKCSYT*	-1.013	0.022387	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27477	SRSLYSIVRRLLLVTANFFG			20	SLAY-screened peptide P5827			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGCGTTCCCTCTATTCTATTGTTCGCCGTCTCCTTCTGGTCACGGCCAACTTTTTCGGCTAA	SRSLYSIVRRLLLVTANFFG*	-1.013	0.002146	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27478	LTFPEMCPCLTY			12	SLAY-screened peptide P5828			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCACGTTTCCTGAGATGTGCCCGTGCCTGACCTATTAGGTTCAGGTGAGCCCTACCCTCTAA	LTFPEMCPCLTY*VQVSPTL*	-1.013	0.003042	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27479	HADGSILRRCVDWPALTFISN			21	SLAY-screened peptide P5829			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATGCGGATGGTTCTATTCTGCGTCGTTGTGTGGACTGGCCGGCCCTTACGTTTATCTCTAAC	HADGSILRRCVDWPALTFISN	-1.013	4e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27480	LACVFLFTPKTRHDCNFSRF			20	SLAY-screened peptide P5830			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTTGCGTGTGTCTTTCTTTTTACCCCGAAGACCCGCCACGATTGTAATTTCTCTAGGTTTTAA	LACVFLFTPKTRHDCNFSRF*	-1.013	0.010636	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27481	RSDRPTSNFTFARPSCALHD			20	SLAY-screened peptide P5831			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTCGGATAGGCCCACCTCGAACTTCACGTTCGCCCGCCCCTCTTGCGCTCTGCACGATTAA	RSDRPTSNFTFARPSCALHD*	-1.013	0.00438	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27482	YLTPSWSIWI			10	SLAY-screened peptide P5832			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCTTACTCCTTCCTGGAGTATCTGGATTTAGCATATGCCCCGTAGTCTGGCTTAGGGTTAA	YLTPSWSIWI*HMPRSLA*G*	-1.013	0.04389	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27483	CTCPNRPRCYSRATTRVLIS			20	SLAY-screened peptide P5833			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTACCTGTCCTAATAGGCCCAGGTGTTACTCTCGGGCTACTACCCGTGTGCTTATTTCTTAA	CTCPNRPRCYSRATTRVLIS*	-1.013	0.001598	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27484	PVNTVPCLILNYADLFLARV			20	SLAY-screened peptide P5834			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGGTTAACACGGTTCCCTGTCTGATTCTGAACTATGCCGATCTGTTCCTCGCCAGGGTCTAA	PVNTVPCLILNYADLFLARV*	-1.012	0.000768	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27485	PNNAGLTRLLSFILLGIVLTN			21	SLAY-screened peptide P5835			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTAACAATGCCGGGTTAACCAGACTTCTTAGCTTTATACTGCTAGGAATTGTTTTGACTAAC	PNNAGLTRLLSFILLGIVLTN	-1.012	1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27486	CRPMTYNHPYYDVISEDNEV			20	SLAY-screened peptide P5836			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGTCCGATGACGTATAATCATCCGTACTATGACGTCATCTCTGAGGACAACGAGGTCTAA	CRPMTYNHPYYDVISEDNEV*	-1.012	2e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27487	RPPCYFITPLSHWTSGVPNI			20	SLAY-screened peptide P5837			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCCTCCCTGTTATTTCATTACGCCCCTTTCCCACTGGACTAGCGGCGTTCCCAACATTTAA	RPPCYFITPLSHWTSGVPNI*	-1.012	0.006316	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27488	SYRLDSQIITYRTNPFSDPP			20	SLAY-screened peptide P5838			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCTATCGCCTGGATAGTCAGATCATTACGTATAGGACTAATCCTTTCAGTGATCCCCCTTAA	SYRLDSQIITYRTNPFSDPP*	-1.011	0.000352	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27489	CYSLDAHNIAHANCWHICCV			20	SLAY-screened peptide P5839			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCTATTCTCTCGACGCCCACAACATTGCTCATGCGAATTGCTGGCATATCTGCTGTGTCTAA	CYSLDAHNIAHANCWHICCV*	-1.011	0.035197	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27490	HFWYTLPMFNRCIYCSSCYL			20	SLAY-screened peptide P5840			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACATTTTTGGTATACCCTGCCGATGTTTAACCGGTGTATCTACTGTTCGTCCTGCTACTTGTAA	HFWYTLPMFNRCIYCSSCYL*	-1.011	0.000145	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27491	SHDVNQWLPDTKIPTCIYYL			20	SLAY-screened peptide P5841			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGTCATGACGTCAATCAGTGGCTCCCTGACACGAAGATCCCTACTTGCATTTACTACCTTTAA	SHDVNQWLPDTKIPTCIYYL*	-1.011	0.029426	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27492	PLPHPCWIEHGNSSPLNWLI			20	SLAY-screened peptide P5842			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTTGCCGCACCCCTGTTGGATCGAGCACGGCAACAGCTCGCCTCTCAACTGGCTCATTTAA	PLPHPCWIEHGNSSPLNWLI*	-1.011	0.026332	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27493	CRCSPRSIRLISALTSALPRN			21	SLAY-screened peptide P5843			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGCCGTTGCAGCCCGCGCTCCATCCGCCTAATCTCGGCCCTGACTAGTGCCCTACCCCGTAAC	CRCSPRSIRLISALTSALPRN	-1.011	0.005859	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27494	PYSVTS			6	SLAY-screened peptide P5844			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTTACTCTGTCACTTCTTAGTGGTGTAACATTGCCAGTGCCACCCACCGCTGTATTTTTTAA	PYSVTS*WCNIASATHRCIF*	-1.011	0.007475	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27495	APRPRVRWPCYPLHLFCLSHS			21	SLAY-screened peptide P5845			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTCCCCGGCCCCGCGTCAGGTGGCCGTGCTACCCCCTGCACCTCTTTTGCCTTAGCCACTCT	APRPRVRWPCYPLHLFCLSHS	-1.011	8.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27496	VHVCPRNAVTPWRFTSMYGL			20	SLAY-screened peptide P5846			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGTTCACGTTTGCCCGCGGAACGCGGTCACGCCTTGGCGCTTTACCTCTATGTACGGCCTTTAA	VHVCPRNAVTPWRFTSMYGL*	-1.01	0.003466	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27497	LSLNFSAPQSFNCTLTSGTAN			21	SLAY-screened peptide P5847			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTGTCTCTCAATTTTAGTGCGCCTCAGAGCTTCAATTGTACTCTCACCTCTGGTACAGCTAAC	LSLNFSAPQSFNCTLTSGTAN	-1.01	2.8e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27498	GYYPIQCQVDPRFVQGLMIP			20	SLAY-screened peptide P5848			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGGTTATTACCCTATTCAGTGTCAGGTCGATCCCCGGTTTGTCCAGGGCCTTATGATCCCCTAA	GYYPIQCQVDPRFVQGLMIP*	-1.01	0.001013	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27499	SDYSISLCVSIICNKYLAVR			20	SLAY-screened peptide P5849			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGACTATTCGATCTCTTTGTGCGTCTCTATCATCTGTAATAAGTACCTTGCGGTGAGGTAA	SDYSISLCVSIICNKYLAVR*	-1.01	3e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27500	EDPAQRTLVPNIPACMGYVL			20	SLAY-screened peptide P5850			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGACCCTGCGCAGCGCACCTTGGTTCCTAATATCCCCGCCTGCATGGGTTACGTTCTCTAA	EDPAQRTLVPNIPACMGYVL*	-1.01	0.006373	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27501	PPADPYMHTITVCCFWSRCGN			21	SLAY-screened peptide P5851			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCTGCCGATCCGTACATGCATACTATTACGGTGTGTTGTTTTTGGAGCCGTTGTGGTAAC	PPADPYMHTITVCCFWSRCGN	-1.01	0.000156	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27502	CSTPYLNHRSGTSPITVQAL			20	SLAY-screened peptide P5852			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGTAGTACTCCTTATCTTAACCACCGTTCTGGTACCTCTCCCATCACGGTCCAGGCGCTGTAA	CSTPYLNHRSGTSPITVQAL*	-1.01	0.03907	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27503	HLLGYYRRFASYLLQFFV			18	SLAY-screened peptide P5853			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACCTCCTGGGTTACTATAGGCGCTTTGCTAGTTACTTGCTTCAGTTTTTTGTGTAGGTCTAA	HLLGYYRRFASYLLQFFV*V*	-1.009	0.007876	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27504	ACTASSDQQFI			11	SLAY-screened peptide P5854			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTTGTACGGCGTCGAGCGACCAGCAGTTTATTTAGATTCTGATTACGAATGTGCTTACTTAA	ACTASSDQQFI*ILITNVLT*	-1.009	0.00022	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27505	PPMDLDPHRLSYLGYTFFWP			20	SLAY-screened peptide P5855			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCGATGGACCTTGACCCCCACCGGCTGTCTTATCTCGGGTATACCTTTTTCTGGCCTTAA	PPMDLDPHRLSYLGYTFFWP*	-1.009	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27506	RVPTRVCPSLPHPEESS			17	SLAY-screened peptide P5856			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGGTTCCTACCCGGGTCTGTCCCTCTCTTCCCCATCCGGAGGAGTCGTCCTAGCACCTGTAA	RVPTRVCPSLPHPEESS*HL*	-1.009	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27507	RYSRYPFYLSPCESTHSTIL			20	SLAY-screened peptide P5857			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCTATTCGCGTTACCCGTTCTACCTCAGTCCGTGCGAGTCTACCCATTCTACCATCCTGTAA	RYSRYPFYLSPCESTHSTIL*	-1.009	0.003968	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27508	DCNLMSDAPYYHANSVYTIA			20	SLAY-screened peptide P5858			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTGTAATCTCATGTCCGACGCTCCTTATTACCATGCTAACTCCGTTTACACCATCGCGTAA	DCNLMSDAPYYHANSVYTIA*	-1.008	5.3e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27509	HSVKYQGQAMPMLNWGSSTR			20	SLAY-screened peptide P5859			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACAGCGTGAAGTACCAGGGCCAGGCCATGCCTATGTTGAACTGGGGTAGTAGCACGCGTTAA	HSVKYQGQAMPMLNWGSSTR*	-1.008	0.000608	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27510	MSFYVPTIHATPHHRNVV			18	SLAY-screened peptide P5860			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTCCTTTTATGTCCCCACGATCCATGCTACGCCGCATCATCGCAACGTGGTCTAGACTTAA	MSFYVPTIHATPHHRNVV*T*	-1.008	0.011549	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27511	LSRVQVATST			10	SLAY-screened peptide P5861			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACTCTCCAGGGTCCAGGTCGCCACCAGCACTTAGATGGTGCTTGCTAGCTATTTGCCCCATTAA	LSRVQVATST*MVLASYLPH*	-1.008	0.026863	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27512	KGSPDVGVVPRWRAVSNWTL			20	SLAY-screened peptide P5862			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGGGGAGTCCTGACGTGGGCGTTGTGCCTCGTTGGCGCGCCGTTTCTAATTGGACTCTGTAA	KGSPDVGVVPRWRAVSNWTL*	-1.008	0.002983	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27513	PPHLSVVVIFSEERRDFIAK			20	SLAY-screened peptide P5863			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCTCATCTTTCGGTAGTTGTCATTTTTAGCGAGGAGCGGCGGGACTTTATTGCGAAGTAA	PPHLSVVVIFSEERRDFIAK*	-1.008	0.021084	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27514	TQHFCSDCCFIPGLQYLDYH			20	SLAY-screened peptide P5864			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACCCAGCATTTTTGTTCTGACTGCTGCTTTATCCCGGGCCTTCAGTACTTGGATTACCACTAA	TQHFCSDCCFIPGLQYLDYH*	-1.008	0.020515	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27515	FTPCLYLPLCDCPFSFYSQFL			21	SLAY-screened peptide P5865			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCACTCCCTGCCTGTACCTACCCCTCTGCGATTGCCCTTTCTCATTTTACTCTCAGTTTTTA	FTPCLYLPLCDCPFSFYSQFL	-1.008	0.003173	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27516	QGSFYCTPYPDYIYSHIVTP			20	SLAY-screened peptide P5866			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGGGCTCTTTTTACTGTACGCCTTATCCGGATTACATCTACTCCCATATTGTTACGCCTTAA	QGSFYCTPYPDYIYSHIVTP*	-1.008	0.000425	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27517	RSCRYLFEPFMTSNESSTWI			20	SLAY-screened peptide P5867			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGGTCGTGTCGCTATCTTTTTGAGCCCTTTATGACTTCCAATGAGTCTAGCACTTGGATTTAA	RSCRYLFEPFMTSNESSTWI*	-1.007	0.000501	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27518	RKHGQDRCMAGTHTPFYVLL			20	SLAY-screened peptide P5868			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAAGCATGGCCAGGATCGGTGCATGGCTGGTACCCATACCCCGTTCTACGTCCTGCTCTAA	RKHGQDRCMAGTHTPFYVLL*	-1.007	0.042943	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27519	NLNHSSSSTIPSGCARFYIA			20	SLAY-screened peptide P5869			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATTTGAACCACTCCTCTAGCAGTACCATCCCCAGTGGCTGCGCCCGGTTCTATATTGCGTAA	NLNHSSSSTIPSGCARFYIA*	-1.007	4.5e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27520	RNTVGHAVGPRLQVSMAANR			20	SLAY-screened peptide P5870			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTAATACGGTGGGCCATGCCGTGGGCCCGCGCCTCCAGGTCTCTATGGCCGCCAACCGCTAA	RNTVGHAVGPRLQVSMAANR*	-1.007	8e-06	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27521	YWNLLSKGLYLACGLISAAY			20	SLAY-screened peptide P5871			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACTGGAATCTCCTGAGCAAGGGTCTGTATTTGGCTTGTGGGCTCATCTCTGCCGCTTACTAA	YWNLLSKGLYLACGLISAAY*	-1.007	0.012225	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27522	RRGAGLYTTTFGADPFHQND			20	SLAY-screened peptide P5872			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGGGGTGCTGGGCTGTACACGACCACGTTTGGCGCGGATCCCTTCCACCAGAACGACTAA	RRGAGLYTTTFGADPFHQND*	-1.007	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27523	WHVQCCFLDYYNSSQITHRI			20	SLAY-screened peptide P5873			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGCACGTCCAGTGTTGTTTCCTGGATTATTATAACAGTTCTCAGATTACGCACCGTATTTAA	WHVQCCFLDYYNSSQITHRI*	-1.007	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27524	NTSHCPVQYTSLNEGLVISL			20	SLAY-screened peptide P5874			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAATACCTCCCATTGCCCTGTGCAGTACACCAGTCTGAATGAGGGTCTGGTTATCTCCTTGTAA	NTSHCPVQYTSLNEGLVISL*	-1.006	0.033533	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27525	ARWTPGST			8	SLAY-screened peptide P5875			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCGCGTTGGACTCCGGGTAGCACCTAGATTTCCAATTCCGACCGCTGTTTTTACATGTCTTAA	ARWTPGST*ISNSDRCFYMS*	-1.006	0.00416	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27526	SAKRPIYISAFHSTNNWHPP			20	SLAY-screened peptide P5876			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCTAAGAGGCCTATCTATATCAGCGCCTTCCATAGTACTAACAACTGGCATCCTCCGTAA	SAKRPIYISAFHSTNNWHPP*	-1.006	0.0	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27527	PPLLILCCAAATPNHFRATR			20	SLAY-screened peptide P5877			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCTCCTCTTCTGATTCTGTGCTGTGCCGCCGCGACGCCTAACCACTTCCGTGCCACACGTTAA	PPLLILCCAAATPNHFRATR*	-1.006	0.003248	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27528	RPFRVTPNIPRPYPWPCRAD			20	SLAY-screened peptide P5878			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCCTTTCAGGGTTACGCCGAACATCCCCCGTCCCTATCCCTGGCCGTGTCGTGCCGATTAA	RPFRVTPNIPRPYPWPCRAD*	-1.006	0.001963	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27529	SECTPHAVTRRLATYNAHEY			20	SLAY-screened peptide P5879			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGAGTGCACGCCCCATGCGGTCACCCGCCGCCTCGCCACCTATAATGCTCACGAGTACTAA	SECTPHAVTRRLATYNAHEY*	-1.006	0.006953	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27530	PPCMLRVPYSWRSHYVENYY			20	SLAY-screened peptide P5880			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCGCCCTGCATGCTCCGTGTTCCCTACAGCTGGCGTAGTCATTACGTTGAGAATTATTATTAA	PPCMLRVPYSWRSHYVENYY*	-1.006	0.002164	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27531	HGEPYHCDTHNLPIATLDRF			20	SLAY-screened peptide P5881			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACGGTGAGCCCTATCATTGTGATACGCATAACCTGCCCATCGCTACGCTGGACCGTTTTTAA	HGEPYHCDTHNLPIATLDRF*	-1.006	1.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27532	DNSPSKTNHCETYTDESDQH			20	SLAY-screened peptide P5882			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGACAACTCCCCTAGTAAGACGAACCATTGTGAGACTTACACCGATGAGTCTGACCAGCACTAA	DNSPSKTNHCETYTDESDQH*	-1.005	0.046828	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27533	SDPFSSLRAVLGGCLSAYLY			20	SLAY-screened peptide P5883			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCGACCCCTTTTCGTCCTTGCGGGCTGTCCTGGGCGGTTGCCTCTCCGCGTACCTCTACTAA	SDPFSSLRAVLGGCLSAYLY*	-1.005	0.017527	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27534	QSACWATDLRVIPFPLIGHN			20	SLAY-screened peptide P5884			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACAGTCGGCGTGTTGGGCCACTGATCTGCGGGTCATCCCCTTCCCGTTGATCGGTCATAATTAA	QSACWATDLRVIPFPLIGHN*	-1.005	0.004516	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27535	EASTQSHTLNSQINCILL			18	SLAY-screened peptide P5885			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGAGGCTAGTACCCAGTCCCATACGCTCAATTCTCAGATTAATTGTATCCTTCTTTAGTTCTAA	EASTQSHTLNSQINCILL*F*	-1.004	0.031005	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27536	MAIHDCIRLNYLRNNLCSAL			20	SLAY-screened peptide P5886			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGGCTATTCATGATTGTATTCGTTTGAACTATCTCCGCAATAATCTCTGCAGCGCGCTCTAA	MAIHDCIRLNYLRNNLCSAL*	-1.004	0.020181	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27537	TGINDHAHSAFFGSNTRLTS			20	SLAY-screened peptide P5887			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACGGGCATTAACGACCATGCCCACTCGGCTTTTTTTGGCTCCAACACCCGGCTGACCTCCTAA	TGINDHAHSAFFGSNTRLTS*	-1.004	0.033961	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27538	MRVPSFPIPFGLHTNNLGNE			20	SLAY-screened peptide P5888			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGCGCGTGCCGAGTTTTCCCATTCCCTTCGGTTTGCATACCAACAACCTGGGCAATGAGTAA	MRVPSFPIPFGLHTNNLGNE*	-1.003	8.1e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27539	ADHSPTISIGRVSISVVVYY			20	SLAY-screened peptide P5889			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGCTGATCATAGCCCCACTATCTCTATTGGGCGTGTTAGTATCAGCGTTGTGGTTTACTATTAA	ADHSPTISIGRVSISVVVYY*	-1.003	0.006221	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27540	FLRSCVNNLFRDR			13	SLAY-screened peptide P5890			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTCCTCCGCTCGTGCGTTAATAACCTCTTTAGGGACAGGTAGTTTGCCTTCAGCCTCAGGTAA	FLRSCVNNLFRDR*FAFSLR*	-1.003	0.014679	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27541	MLDNRGGVPLPNSRGPYCTA			20	SLAY-screened peptide P5891			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGTTGGATAATCGTGGTGGCGTCCCGCTGCCGAACTCGAGGGGCCCTTATTGTACGGCGTAA	MLDNRGGVPLPNSRGPYCTA*	-1.003	0.000675	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27542	KHGIVGDLSMCISGNIS			17	SLAY-screened peptide P5892			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAAGCATGGTATTGTTGGTGACTTGAGTATGTGCATCAGTGGTAATATCAGCTAGCATTTTTAA	KHGIVGDLSMCISGNIS*HF*	-1.003	0.029899	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27543	PLSAAGLYEPYSSFPPFMCR			20	SLAY-screened peptide P5893			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCCTCAGCGCTGCGGGGCTTTATGAGCCCTACAGTTCGTTTCCTCCGTTTATGTGTCGTTAA	PLSAAGLYEPYSSFPPFMCR*	-1.002	3.4e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27544	TCKIGMHDILQHAILIMHSP			20	SLAY-screened peptide P5894			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTTGTAAGATTGGTATGCATGACATCCTCCAGCACGCTATTCTCATCATGCACTCCCCCTAA	TCKIGMHDILQHAILIMHSP*	-1.002	0.020893	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27545	IKFMETFNDSIFLINHNPRN			20	SLAY-screened peptide P5895			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATCAAGTTCATGGAGACCTTCAACGATTCCATCTTCCTTATTAATCACAACCCTCGGAACTAA	IKFMETFNDSIFLINHNPRN*	-1.002	0.012012	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27546	RQYPTMAAPHHRSNCASALP			20	SLAY-screened peptide P5896			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGTCAGTATCCCACCATGGCTGCCCCTCATCATCGGTCGAACTGCGCTTCCGCGCTTCCTTAA	RQYPTMAAPHHRSNCASALP*	-1.002	0.000542	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27547	YLTHPSIECLMHHYSFHNHT			20	SLAY-screened peptide P5897			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATATTTGACCCACCCCTCTATTGAGTGCTTGATGCATCACTATTCTTTTCATAATCACACCTAA	YLTHPSIECLMHHYSFHNHT*	-1.002	0.00156	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27548	SPRVRSIDCWLHNKYSLY			18	SLAY-screened peptide P5898			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCCCCCCGTGTCCGTAGTATCGACTGCTGGCTCCATAACAAGTACAGCCTTTACTAGTAACTG	SPRVRSIDCWLHNKYSLY**L	-1.002	0.039257	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27549	FLRLIGALPQCITRPLHNPP			20	SLAY-screened peptide P5899			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATTTCTCCGTCTCATCGGTGCGCTGCCTCAGTGCATCACGCGGCCCTTGCATAACCCTCCGTAA	FLRLIGALPQCITRPLHNPP*	-1.002	0.006971	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27550	SAVRNPHCALDYWAGSCYCY			20	SLAY-screened peptide P5900			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAAGCGCTGTCCGTAACCCCCACTGTGCCCTGGACTACTGGGCTGGCTCGTGTTATTGTTATTAA	SAVRNPHCALDYWAGSCYCY*	-1.002	0.043073	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27551	WCVPVFGFATYSIYCLPFSA			20	SLAY-screened peptide P5901			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATGGTGCGTTCCGGTGTTTGGGTTCGCGACTTACTCGATCTATTGTCTTCCTTTTTCGGCCTAA	WCVPVFGFATYSIYCLPFSA*	-1.001	0.02078	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27552	PDPVPWGLRFFQLPPCSRLD			20	SLAY-screened peptide P5902			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACCCGATCCTGTCCCTTGGGGGTTGCGCTTCTTCCAGCTCCCTCCGTGTTCTCGCCTTGACTAA	PDPVPWGLRFFQLPPCSRLD*	-1.001	0.01035	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27553	MIVPYSENYGYYLCCCALSA			20	SLAY-screened peptide P5903			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAATGATCGTGCCCTATAGTGAGAATTACGGCTATTATCTCTGTTGCTGTGCCCTTTCCGCGTAA	MIVPYSENYGYYLCCCALSA*	-1.001	0.015523	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27554	TRCTIMPYWDIYRSPTYIIL			20	SLAY-screened peptide P5904			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAACTCGTTGTACGATTATGCCCTACTGGGACATTTATCGTTCGCCCACCTACATCATTCTCTAA	TRCTIMPYWDIYRSPTYIIL*	-1.001	0.001136	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27555	SAVPLINFPNHNNSLIIFIH			20	SLAY-screened peptide P5905			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATCGGCGGTCCCGCTCATCAATTTTCCGAATCATAATAACTCGTTGATCATCTTTATTCATTAA	SAVPLINFPNHNNSLIIFIH*	-1.001	0.048531	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27556	RRPCYSSYYLFM			12	SLAY-screened peptide P5906			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACGCCGCCCTTGCTATAGCAGCTATTACCTCTTTATGTAGGGCCCGGCCAATTTCGCTTATTAA	RRPCYSSYYLFM*GPANFAY*	-1.001	5.9e-05	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27557	DYSPEKQYTPQNNTLNCLVV			20	SLAY-screened peptide P5907			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGAGATTATTCCCCGGAGAAGCAGTATACCCCTCAGAATAATACCCTGAATTGCTTGGTTGTTTAA	DYSPEKQYTPQNNTLNCLVV*	-1.001	0.018869	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27558	YHIVGYKVTYAKDNIIA			17	SLAY-screened peptide P5908			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGATACCACATTGTGGGTTATAAGGTTACGTATGCTAAGGATAACATTATCGCTTAGGCGTGTTAA	YHIVGYKVTYAKDNIIA*AC*	-1.001	0.031045	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP27559	HSFMPNCFL			9	SLAY-screened peptide P5909			Screening by SLAY	Anti-recombinant genetically engineered E. coli								Linear						29307492	Cell. 2018 Jan 25;172(3):618-628.e13. doi: 10.1016/j.cell.2017.12.009. Epub 2018 Jan 4.	Ashley T Tucker, Sean P Leonard, Cory D DuBois, Gregory A Knauf, Ashley L Cunningham, Claus O Wilke, M Stephen Trent, Bryan W Davies	Discovery of Next-Generation Antimicrobials through Bacterial Self-Screening of Surface-Displayed Peptide Libraries	Candidate AMP	GGAGGACACTCCTTTATGCCCAATTGCTTCCTGTAGTGCAACCGGGCTTAGGCGCGTCTTAAGTACTAA	HSFMPNCFL*CNRA*ARLKY*	-1.0	0.025977	"Batch screening of peptides using SLAY system can be achieved by first constructing a random library using random PCR primers that flank the peptide region (i), followed by collection of transformants, plasmid isolation, and subsequent transformation into a bacterial strain of interest. Next, the library is grown in culture and induced (ii). Peptides with antimicrobial activity (red) will drop out of the population (iii). Next-generation sequencing of the initial input at time zero and output (iv) at a pre-defined number of hours provides a read out of sequencing counts (v). From this information, top hits can be identified and tested. Further libraries can be constructed based on the identified top hits and the process can be repeated.
Log2 fold values indicate the degree to which the peptides were removed from the population. If the remaining antimicrobial peptides after sequencing show a log2 fold change of -1 or lower, indicating they were removed from the population over the time course. lfcMLE and Padj represent log2 FoldChange MLE and p values adjusted, respectively."
DRAMP28986	ⓍIKKⓍLKSAKKFVKAFK	XIKKXLKSAKKFVKAFK	GIGKFLHSAKKFGKAFVGEIMNS	17	peptide 2 (Derived from Mag2)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM	[Ref.33466998] All peptides with side-chain stapling showed negative maxima at around 208 and 222 nm, indicating that the peptides formed a stablized helical structure.	Nor found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria	"[Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC = 3.125 μM). 
##Gram-negative bacteria: Escherichia coli (MIC = 1.56 μM), Pseudomonas aeruginosa (MIC = 1.56 μM), 
multiple-drug resistant P.aeruginosa (MIC = 1.56 μM)"	[Ref.33466998] It has hemolysis against human red blood cells at 50 μM	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 1 and 5) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (1) and Ⓧ (5) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	33466998	Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.	Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu	Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence	Stapled AMP					
DRAMP28987	GIKKⓍLKSⓍKKFVKAFK	GIKKXLKSXKKFVKAFK	GIGKFLHSAKKFGKAFVGEIMNS	17	peptide 3 (Derived from Mag2)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM	[Ref.33466998] All peptides with side-chain stapling showed negative maxima at around 208 and 222 nm, indicating that the peptides formed a stablized helical structure.	Nor found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria	"[Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC = 3.125 μM). 
##Gram-negative bacteria: Escherichia coli (MIC = 1.56 μM), Pseudomonas aeruginosa (MIC = 3.125 μM), 
multiple-drug resistant P.aeruginosa (MIC = 0.78 μM)"	[Ref.33466998] It has hemolysis against human red blood cells at 6.25 μM	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 5 and 9) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (5) and Ⓧ (9) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	33466998	Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.	Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu	Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence	Stapled AMP					
DRAMP28988	GIKKFLKⓍAKKⓍVKAFK	GIKKFLKXAKKXVKAFK	GIGKFLHSAKKFGKAFVGEIMNS	17	peptide 4 (Derived from Mag2)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM	[Ref.33466998] All peptides with side-chain stapling showed negative maxima at around 208 and 222 nm, indicating that the peptides formed a stablized helical structure.	Nor found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria	"[Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC = 6.25 μM). 
##Gram-negative bacteria: Escherichia coli (MIC = 1.56 μM), Pseudomonas aeruginosa (MIC = 1.56 μM), 
multiple-drug resistant P.aeruginosa (MIC = 0.78 μM)"	[Ref.33466998] It has hemolysis against human red blood cells at 3.125 μM	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 8 and 12) in sequence indicates (S)-4-pentenyl alanine. ②Ⓧ (8) and Ⓧ (12) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	33466998	Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.	Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu	Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence	Stapled AMP					
DRAMP28989	GIKKFLKSⓍKKFⓍKAFK	GIKKFLKSXKKFXKAFK	GIGKFLHSAKKFGKAFVGEIMNS	17	peptide 5 (Derived from Mag2)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM	[Ref.33466998] All peptides with side-chain stapling showed negative maxima at around 208 and 222 nm, indicating that the peptides formed a stablized helical structure.	Nor found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria	"[Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC = 12.5 μM). 
##Gram-negative bacteria: Escherichia coli (MIC = 1.56 μM), Pseudomonas aeruginosa (MIC = 6.25 μM), 
multiple-drug resistant P.aeruginosa (MIC = 3.125 μM)"	[Ref.33466998] It has hemolysis against human red blood cells at 1.56 μM	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 9 and 13) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	33466998	Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.	Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu	Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence	Stapled AMP					
DRAMP28990	GIKKFLKSAKKⓍVKAⓍK	GIKKFLKSAKKXVKAXK	GIGKFLHSAKKFGKAFVGEIMNS	17	peptide 6 (Derived from Mag2)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM	[Ref.33466998] All peptides with side-chain stapling showed negative maxima at around 208 and 222 nm, indicating that the peptides formed a stablized helical structure.	Nor found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria	"[Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC = 3.125 μM). 
##Gram-negative bacteria: Escherichia coli (MIC = 1.56 μM), Pseudomonas aeruginosa (MIC = 1.56 μM), 
multiple-drug resistant P.aeruginosa (MIC = 0.78 μM)"	[Ref.33466998] It has hemolysis against human red blood cells at 3.125 μM	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 12 and 16) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (12) and Ⓧ (16) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	33466998	Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.	Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu	Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence	Stapled AMP					
DRAMP28991	GIKKⓏLKSAKKⓍVKAFK	GIKKZLKSAKKXVKAFK	GIGKFLHSAKKFGKAFVGEIMNS	17	peptide 7 (Derived from Mag2)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM	[Ref.33466998] All peptides with side-chain stapling showed negative maxima at around 208 and 222 nm, indicating that the peptides formed a stablized helical structure.	Nor found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria	"[Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC = 25 μM). 
##Gram-negative bacteria: Escherichia coli (MIC = 3.125 μM), Pseudomonas aeruginosa (MIC = 3.125 μM), 
multiple-drug resistant P.aeruginosa (MIC = 1.56 μM)"	[Ref.33466998] It has hemolysis against human red blood cells at 6.25 μM	Cyclic (Stapled)	Free	Amidation	①The Ⓩ (position: 5) in sequence indicates (R)-7-octenyl alanine. ②The Ⓧ (position: 12) in sequence indicates (S)-4-pentenyl alanine. ③ Ⓩ (5) and Ⓧ (12) are cross-linked by hydrocarbon stapling through a undec-4-enyl staple.	L	No cytotoxicity information found in the reference	33466998	Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.	Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu	Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence	Stapled AMP					
DRAMP28992	GIKKFLKⓏAKKFVKⓍFK	GIKKFLKZAKKFVKXFK	GIGKFLHSAKKFGKAFVGEIMNS	17	peptide 8 (Derived from Mag2)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM	[Ref.33466998] All peptides with side-chain stapling showed negative maxima at around 208 and 222 nm, indicating that the peptides formed a stablized helical structure.	Nor found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria	"[Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC > 50 μM). 
##Gram-negative bacteria: Escherichia coli (MIC = 6.25 μM), Pseudomonas aeruginosa (MIC > 50 μM), 
multiple-drug resistant P.aeruginosa (MIC = 3.125 μM)"	[Ref.33466998] It has hemolysis against human red blood cells at 0.19 μM	Cyclic (Stapled)	Free	Amidation	①The Ⓩ (position: 8) in sequence indicates (R)-7-octenyl alanine. ②The Ⓧ (position: 15) in sequence indicates (S)-4-pentenyl alanine. ③ Ⓩ (8) and Ⓧ (15) are cross-linked by hydrocarbon stapling through a undec-4-enyl staple.	L	No cytotoxicity information found in the reference	33466998	Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.	Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu	Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence	Stapled AMP					
DRAMP28993	GIKKFLKSⓏKKFVKAⓍK	GIKKFLKSZKKFVKAXK	GIGKFLHSAKKFGKAFVGEIMNS	17	peptide 9 (Derived from Mag2)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM	[Ref.33466998] All peptides with side-chain stapling showed negative maxima at around 208 and 222 nm, indicating that the peptides formed a stablized helical structure.	Nor found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria	"[Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC = 6.25 μM). 
##Gram-negative bacteria: Escherichia coli (MIC = 3.125 μM), Pseudomonas aeruginosa (MIC = 6.25 μM), 
multiple-drug resistant P.aeruginosa (MIC = 3.125 μM)"	[Ref.33466998] It has hemolysis against human red blood cells at 3.125 μM	Cyclic (Stapled)	Free	Amidation	①The Ⓩ (position: 9) in sequence indicates (R)-7-octenyl alanine. ②The Ⓧ (position: 16) in sequence indicates (S)-4-pentenyl alanine. ③ Ⓩ (9) and Ⓧ (16) are cross-linked by hydrocarbon stapling through a undec-4-enyl staple.	L	No cytotoxicity information found in the reference	33466998	Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.	Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu	Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence	Stapled AMP					
DRAMP28994	ⓍIKKⓍLKSAKKFVKAFK	XIKKXLKSAKKFVKAFK	GIGKFLHSAKKFGKAFVGEIMNS	17	peptide C6-2 (Derived from Mag2)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM	[Ref.33466998] Peptide C6-2 and C12-2 showed a curve similar to that of peptide 2	Nor found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria	"[Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC = 1.56 μM). 
##Gram-negative bacteria: Escherichia coli (MIC = 1.56 μM), Pseudomonas aeruginosa (MIC = 3.125 μM), 
multiple-drug resistant P.aeruginosa (MIC = 3.125 μM)"	[Ref.33466998] It has hemolysis against human red blood cells at 12.5 μM	Cyclic (Stapled)	Hexanoylation	Amidation	①The Ⓧ (position: 1 and 5) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (1) and Ⓧ (5) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	33466998	Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.	Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu	Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence	Stapled AMP					
DRAMP28995	ⓍIKKⓍLKSAKKFVKAFK	XIKKXLKSAKKFVKAFK	GIGKFLHSAKKFGKAFVGEIMNS	17	peptide C12-2 (Derived from Mag2)	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-helix in 20 mM PBS solution (pH = 7.4) with 1% SDS at peptide concentrations of 100 μM	[Ref.33466998] Peptide C6-2 and C12-2 showed a curve similar to that of peptide 2	Nor found	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria	"[Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC = 6.25 μM). 
##Gram-negative bacteria: Escherichia coli (MIC = 6.25 μM), Pseudomonas aeruginosa (MIC = 12.5 μM), 
multiple-drug resistant P.aeruginosa (MIC = 25 μM)"	[Ref.33466998] It has hemolysis against human red blood cells at 0.78 μM	Cyclic (Stapled)	Laurylation	Amidation	①The Ⓧ (position: 1 and 5) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (1) and Ⓧ (5) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	33466998	Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.	Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu	Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence	Stapled AMP					
DRAMP28996	ⓍIKKⓍLKSAKKFVKAFK	XIKKXLKSAKKFVKAFK	GIGKFLHSAKKFGKAFVGEIMNS	17	peptide C18-2 (Derived from Mag2)	No entry found		Synthetic construct	Non-Antimicrobial		Unknown	[Ref.33466998] The circular dichroism (CD) spectral analysis of C18-2 could not be performed because of its poor solubility in the aqueous buffer solution.	Nor found	Function: Antibacterial activity against bacteria is not significant under the concentration of 50 μM.	"[Ref.33466998] Gram-positive bacteria: Staphylococcus aureus (MIC > 50 μM). 
##Gram-negative bacteria: Escherichia coli (MIC > 50 μM), Pseudomonas aeruginosa (MIC > 50 μM), 
multiple-drug resistant P.aeruginosa (MIC > 50 μM)"	[Ref.33466998] It has hemolysis against human red blood cells at 0.78 μM	Cyclic (Stapled)	Stearylation	Amidation	①The Ⓧ (position: 1 and 5) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (1) and Ⓧ (5) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	33466998	Molecules. 2021 Jan 16;26(2):444. doi: 10.3390/molecules26020444.	Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu	Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence	Stapled AMP					
DRAMP28998	RⓍWWRⓍW	RXWWRXW	RWWWRWW	7	Stapled heptapeptide 2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+		Helicity = 61.3% in 10 mM sodium phosphate buffer (pH 7.4) at peptide concentrations of 100 μM	CD spectroscopy was used to characterize the secondary structure of five unstapled heptapeptides and their stapled counterparts in phosphate buffer, indicating a significant increase in peptide helical content upon the stapling, with helicity change from h = 14.1% - 33.7% (for unstapled peptides) to h = 58.9%-75.1% (for stapled peptides).	Not found	"Function: Antibacterial activity against Gram-positive bacteria.
The stapled peptide was much more active against bacteria than the unstapled one."	Gram-positive bacteria: Staphylococcus aureus ATCC25923 (MIC = 19 ± 4 μg/mL). Methicillin-resistant Staphylococcus aureus (MRSA) (MIC > 25 ± 6 μg/mL)	No hemolytic activity information found.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	PubMed ID is not available	Int J Pept Res Ther. 2019 Nov 14; 26(4):1711–1719. doi: 10.1007/s10989-019-09964-7.	Zhixia Chen, Xiuli Yu, Aiying Zhang, Fangfang Wang, Yankun Xing	De Novo Hydrocarbon-Stapling Design of Single-Turn α-Helical Antimicrobial Peptides	Stapled AMP					
DRAMP29000	WⓍKWWⓍK	WXKWWXK	WWKWWWK	7	Stapled heptapeptide 4	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+		Helicity = 69.8% in 10 mM sodium phosphate buffer (pH 7.4) at peptide concentrations of 100 μM	CD spectroscopy was used to characterize the secondary structure of five unstapled heptapeptides and their stapled counterparts in phosphate buffer, indicating a significant increase in peptide helical content upon the stapling, with helicity change from h = 14.1% - 33.7% (for unstapled peptides) to h = 58.9%-75.1% (for stapled peptides).	Not found	"Function: Antibacterial activity against Gram-positive bacteria.
The stapled peptide was much more active against bacteria than the unstapled one."	Gram-positive bacteria: Staphylococcus aureus ATCC25923 (MIC = 8.3 ± 1.8 μg/mL). Methicillin-resistant Staphylococcus aureus (MRSA) (MIC = 14 ± 3 μg/mL)	No hemolytic activity information found.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	PubMed ID is not available	Int J Pept Res Ther. 2019 Nov 14; 26(4):1711–1719. doi: 10.1007/s10989-019-09964-7.	Zhixia Chen, Xiuli Yu, Aiying Zhang, Fangfang Wang, Yankun Xing	De Novo Hydrocarbon-Stapling Design of Single-Turn α-Helical Antimicrobial Peptides	Stapled AMP					
DRAMP29002	LⓍLRLⓍR	LXLRLXR	LLLRLLR	7	Stapled heptapeptide 6	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+		Helicity = 75.1% in 10 mM sodium phosphate buffer (pH 7.4) at peptide concentrations of 100 μM	CD spectroscopy was used to characterize the secondary structure of five unstapled heptapeptides and their stapled counterparts in phosphate buffer, indicating a significant increase in peptide helical content upon the stapling, with helicity change from h = 14.1% - 33.7% (for unstapled peptides) to h = 58.9%-75.1% (for stapled peptides).	Not found	"Function: Antibacterial activity against Gram-positive bacteria.
The stapled peptide was much more active against bacteria than the unstapled one."	Gram-positive bacteria: Staphylococcus aureus ATCC25923 (MIC = 24 ± 4 μg/mL). Methicillin-resistant Staphylococcus aureus (MRSA) (MIC = 15 ± 3 μg/mL)	No hemolytic activity information found.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	PubMed ID is not available	Int J Pept Res Ther. 2019 Nov 14; 26(4):1711–1719. doi: 10.1007/s10989-019-09964-7.	Zhixia Chen, Xiuli Yu, Aiying Zhang, Fangfang Wang, Yankun Xing	De Novo Hydrocarbon-Stapling Design of Single-Turn α-Helical Antimicrobial Peptides	Stapled AMP					
DRAMP29004	WⓍRRWⓍR	WXRRWXR	WWRRWWR	7	Stapled heptapeptide 8	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+		Helicity = 58.9% in 10 mM sodium phosphate buffer (pH 7.4) at peptide concentrations of 100 μM	CD spectroscopy was used to characterize the secondary structure of five unstapled heptapeptides and their stapled counterparts in phosphate buffer, indicating a significant increase in peptide helical content upon the stapling, with helicity change from h = 14.1% - 33.7% (for unstapled peptides) to h = 58.9%-75.1% (for stapled peptides).	Not found	"Function: Antibacterial activity against Gram-positive bacteria.
The stapled peptide was much more active against bacteria than the unstapled one."	Gram-positive bacteria: Staphylococcus aureus ATCC25923 (MIC = 56 ± 12 μg/mL). Methicillin-resistant Staphylococcus aureus (MRSA) (MIC = 72 ± 18 μg/mL)	No hemolytic activity information found.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicate (S)-4-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	PubMed ID is not available	Int J Pept Res Ther. 2019 Nov 14; 26(4):1711–1719. doi: 10.1007/s10989-019-09964-7.	Zhixia Chen, Xiuli Yu, Aiying Zhang, Fangfang Wang, Yankun Xing	De Novo Hydrocarbon-Stapling Design of Single-Turn α-Helical Antimicrobial Peptides	Stapled AMP					
DRAMP29007	GⓍFAVⓍKKVASVIKGL	GXFAVXKKVASVIKGL	GLFAVIKKVASVIKGL	16	A4K14-citropin1.1-Sp1	No entry found		Synthetic construct	Antimicrobial, Anticancer		Helicity = 89.8% in 50% 2,2,2-trifluoroethanol (TFE) aqueous solution (0.1mg/mL)	[Ref.33363118] ①CD analysis indicates that the helicity of intial A4K14-citropin 1.1 was 61.5% and that of the stapled peptides ranged from 13.6 to 89.8%. ②Among them, A4K14-citropin 1.1-Sp1 and A4K14-citropin 1.1-Sp4 displayed the top 2 degrees of helicity (89.8 and 85.3%, respectively) in the aqueous solution and acquired 1.46- and 1.38-fold improvements compared to A4K14-citropin 1.1, respectively.	Not found	"Function: Antitumor activity against A549, HCT116 and HepG2 cancer cells.
The hydrolysis enzyme (α-chymotrypsin-mediated) degradation half-life of A4K14-citropin1.1-Sp1  is over 10 hours. A4K14-citropin1.1-Sp1 and A4K14-citropin1.1-Sp4 are much more stable than other A4K14-citropin1.1 stapling derivatives in Ref.33363118."	[Ref.33363118] Cancer cell lines: C4-2B (IC50 = 8.94 μM), A549 (IC50 = 12.48 μM), U87 (IC50 = 11.88 μM), MCF-7 (11.26 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	① The Ⓧ (position: 2 and 6) in sequence indicate (S)-2-(4-pentenyl)alanine. ② Ⓧ (2) and Ⓧ (6) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	33363118	Front Chem. 2020 Dec 10;8:616147. doi: 10.3389/fchem.2020.616147. eCollection 2020.	Nan Wang, Gang Xie, Chao Liu, Wei Cong, Shipeng He, Yinghua Li, Li Fan, Hong-Gang Hu 	Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides	Stapled AMP					
DRAMP29008	GLFAⓍIKKⓍASVIKGL	GLFAXIKKXASVIKGL	GLFAVIKKVASVIKGL	16	A4K14-citropin1.1-Sp2	No entry found		Synthetic construct	Antimicrobial, Anticancer		Helicity = 66.1% in 50% 2,2,2-trifluoroethanol (TFE) aqueous solution (0.1mg/mL)	[Ref.33363118] CD analysis indicates that the helicity of intial A4K14-citropin 1.1 was 61.5% and that of the stapled peptides ranged from 13.6 to 89.8%.	Not found	Function: Antitumor activity against A549, HCT116 and HepG2 cancer cells.	[Ref.33363118] Cancer cell lines: C4-2B (IC50 = 10.14 μM), A549 (IC50 = 12.55 μM), U87 (IC50 = 14.76 μM), MCF-7 (12.65 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	① The Ⓧ (position: 5 and 9) in sequence indicate (S)-2-(4-pentenyl)alanine. ② Ⓧ (5) and Ⓧ (9) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	33363118	Front Chem. 2020 Dec 10;8:616147. doi: 10.3389/fchem.2020.616147. eCollection 2020.	Nan Wang, Gang Xie, Chao Liu, Wei Cong, Shipeng He, Yinghua Li, Li Fan, Hong-Gang Hu 	Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides	Stapled AMP					
DRAMP29009	GLFAVⓍKKVⓍSVIKGL	GLFAVXKKVXSVIKGL	GLFAVIKKVASVIKGL	16	A4K14-citropin1.1-Sp3	No entry found		Synthetic construct	Antimicrobial, Anticancer		Helicity = 49.2% in 50% 2,2,2-trifluoroethanol (TFE) aqueous solution (0.1mg/mL)	[Ref.33363118] CD analysis indicates that the helicity of intial A4K14-citropin 1.1 was 61.5% and that of the stapled peptides ranged from 13.6 to 89.8%.	Not found	Function: Antitumor activity against A549, HCT116 and HepG2 cancer cells.	[Ref.33363118] Cancer cell lines: C4-2B (IC50 = 17.89 μM), A549 (IC50 = 12.11 μM), U87 (IC50 = 11.93 μM), MCF-7 (11.92 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	① The Ⓧ (position: 6 and 10) in sequence indicate (S)-2-(4-pentenyl)alanine. ② Ⓧ (6) and Ⓧ (10) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	33363118	Front Chem. 2020 Dec 10;8:616147. doi: 10.3389/fchem.2020.616147. eCollection 2020.	Nan Wang, Gang Xie, Chao Liu, Wei Cong, Shipeng He, Yinghua Li, Li Fan, Hong-Gang Hu 	Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides	Stapled AMP					
DRAMP29010	GLFAVIKKⓍASVⓍKGL	GLFAVIKKXASVXKGL	GLFAVIKKVASVIKGL	16	A4K14-citropin1.1-Sp4	No entry found		Synthetic construct	Antimicrobial, Anticancer		Helicity = 85.3% in 50% 2,2,2-trifluoroethanol (TFE) aqueous solution (0.1mg/mL)	[Ref.33363118] ①CD analysis indicates that the helicity of intial A4K14-citropin 1.1 was 61.5% and that of the stapled peptides ranged from 13.6 to 89.8%.②Among them, A4K14-citropin 1.1-Sp1 and A4K14-citropin 1.1-Sp4 displayed the top 2 degrees of helicity (89.8 and 85.3%, respectively) in the aqueous solution and acquired 1.46- and 1.38-fold improvements compared to A4K14-citropin 1.1, respectively.	Not found	"Function: Antitumor activity against A549, HCT116 and HepG2 cancer cells.
The hydrolysis enzyme (α-chymotrypsin-mediated) degradation half-life of A4K14-citropin1.1-Sp1  is over 10 hours. A4K14-citropin1.1-Sp1 and A4K14-citropin1.1-Sp4 are much more stable than other A4K14-citropin1.1 stapling derivatives in Ref.33363118."	[Ref.33363118] Cancer cell lines: C4-2B (IC50 = 8.90 μM), A549 (IC50 = 10.51 μM), U87 (IC50 = 7.277 μM), MCF-7 (10.49 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	① The Ⓧ (position: 9 and 13) in sequence indicate (S)-2-(4-pentenyl)alanine. ② Ⓧ (9) and Ⓧ (13) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	33363118	Front Chem. 2020 Dec 10;8:616147. doi: 10.3389/fchem.2020.616147. eCollection 2020.	Nan Wang, Gang Xie, Chao Liu, Wei Cong, Shipeng He, Yinghua Li, Li Fan, Hong-Gang Hu 	Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides	Stapled AMP					
DRAMP29011	GLFAVIKKVAⓍVIKⓍL	GLFAVIKKVAXVIKXL	GLFAVIKKVASVIKGL	16	A4K14-citropin1.1-Sp5	No entry found		Synthetic construct	Antimicrobial, Anticancer		Helicity = 49.4% in 50% 2,2,2-trifluoroethanol (TFE) aqueous solution (0.1mg/mL)	[Ref.33363118] CD analysis indicates that the helicity of intial A4K14-citropin 1.1 was 61.5% and that of the stapled peptides ranged from 13.6 to 89.8%.	Not found	Function: Antitumor activity against A549, HCT116 and HepG2 cancer cells.	[Ref.33363118] Cancer cell lines: C4-2B (IC50 = 11.90 μM), A549 (IC50 = 9.899 μM), U87 (IC50 = 8.229 μM), MCF-7 (12.42 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	① The Ⓧ (position: 11 and 15) in sequence indicate (S)-2-(4-pentenyl)alanine. ② Ⓧ (11) and Ⓧ (15) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	33363118	Front Chem. 2020 Dec 10;8:616147. doi: 10.3389/fchem.2020.616147. eCollection 2020.	Nan Wang, Gang Xie, Chao Liu, Wei Cong, Shipeng He, Yinghua Li, Li Fan, Hong-Gang Hu 	Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides	Stapled AMP					
DRAMP29012	GⓏFAVIKKⓍASVIKGL	GZFAVIKKXASVIKGL	GLFAVIKKVASVIKGL	16	A4K14-citropin1.1-Sp6	No entry found		Synthetic construct	Antimicrobial, Anticancer		Helicity = 34.9% in 50% 2,2,2-trifluoroethanol (TFE) aqueous solution (0.1mg/mL)	[Ref.33363118] CD analysis indicates that the helicity of intial A4K14-citropin 1.1 was 61.5% and that of the stapled peptides ranged from 13.6 to 89.8%.	Not found	Function: Antitumor activity against A549, HCT116 and HepG2 cancer cells.	[Ref.33363118] Cancer cell lines: C4-2B (IC50 = 35.84 μM), A549 (IC50 = 30.19 μM), U87 (IC50 = 34.49 μM), MCF-7 (23.78 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	① The Ⓩ (position: 2) in sequence indicates (R)-2-(7-octenyl)alanine. ② The Ⓧ (position: 9) in sequence indicates (S)-2-(4-pentenyl)alanine. ③ Ⓩ and Ⓧ are cross-linked by ring-closing metathesis through a undec-4-enyl staple.	L	No cytotoxicity information found in the reference	33363118	Front Chem. 2020 Dec 10;8:616147. doi: 10.3389/fchem.2020.616147. eCollection 2020.	Nan Wang, Gang Xie, Chao Liu, Wei Cong, Shipeng He, Yinghua Li, Li Fan, Hong-Gang Hu 	Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides	Stapled AMP					
DRAMP29013	GLFAVⓏKKVASVⓍKGL	GLFAVZKKVASVXKGL	GLFAVIKKVASVIKGL	16	A4K14-citropin1.1-Sp7	No entry found		Synthetic construct	Antimicrobial, Anticancer		Helicity = 13.6% in 50% 2,2,2-trifluoroethanol (TFE) aqueous solution (0.1mg/mL)	[Ref.33363118] CD analysis indicates that the helicity of intial A4K14-citropin 1.1 was 61.5% and that of the stapled peptides ranged from 13.6 to 89.8%.	Not found	Function: Antitumor activity against A549, HCT116 and HepG2 cancer cells.	[Ref.33363118] Cancer cell lines: C4-2B (IC50 = 10.23 μM), A549 (IC50 = 16.37 μM), U87 (IC50 = 14.72 μM), MCF-7 (12.1 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	① The Ⓩ (position: 6) in sequence indicates (R)-2-(7-octenyl)alanine. ② The Ⓧ (position: 13) in sequence indicates (S)-2-(4-pentenyl)alanine. ③ Ⓩ and Ⓧ are cross-linked by ring-closing metathesis through a undec-4-enyl staple.	L	No cytotoxicity information found in the reference	33363118	Front Chem. 2020 Dec 10;8:616147. doi: 10.3389/fchem.2020.616147. eCollection 2020.	Nan Wang, Gang Xie, Chao Liu, Wei Cong, Shipeng He, Yinghua Li, Li Fan, Hong-Gang Hu 	Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides	Stapled AMP					
DRAMP29015	IKLSPⓍTKDⓍLKKVLKGAIKGAIAVAKMV	IKLSPXTKDXLKKVLKGAIKGAIAVAKMV	IKLSPETKDNLKKVLKGAIKGAIAVAKMV	29	H-2	No entry found		Synthetic construct	Antimicrobial, Anticancer		α-helical (most likely)	No detailed structure description found.	Not found	"Function: Anticancer activity. 
Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays"	[Ref.30789695] Cancer cell lines: A549 (IC50 = 4.26 ± 0.52 μM), HCT116 (IC50 = 3.54 ± 0.72 μM), HepG2 (IC50 = 1.60 ± 0.24 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Free	Amidation	① The Ⓧ (position: 6 and 10) in sequence indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ② Ⓧ (6) and Ⓧ (10) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30789695	ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.	Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao	Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy	Stapled AMP					
DRAMP29016	IKLSPETKDNLKKVLKGⓍIKGⓍIAVAKMV	IKLSPETKDNLKKVLKGXIKGXIAVAKMV	IKLSPETKDNLKKVLKGAIKGAIAVAKMV	29	H-5	No entry found		Synthetic construct	Antimicrobial, Anticancer		α-helical (most likely)	No detailed structure description found.	Not found	"Function: Anticancer activity. 
Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays"	[Ref.30789695] Cancer cell lines: A549 (IC50 = 7.35 ± 0.22 μM), HCT116 (IC50 = 4.16 ± 0.21 μM), HepG2 (IC50 = 2.82 ± 0.23 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Free	Amidation	① The Ⓧ (position: 18 and 22) in sequence indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ② Ⓧ (18) and Ⓧ (22) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30789695	ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.	Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao	Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy	Stapled AMP					
DRAMP29017	IKLSPⓍTKDⓍLKKVLKGⓍIKGⓍIAVAKMV	IKLSPXTKDXLKKVLKGXIKGXIAVAKMV	IKLSPETKDNLKKVLKGAIKGAIAVAKMV	29	H-10	No entry found		Synthetic construct	Antimicrobial, Anticancer		α-helical (most likely)	No detailed structure description found.	Not found	"Function: Anticancer activity. 
Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays"	[Ref.30789695] Cancer cell lines: A549 (IC50 = 3.59 ± 0.12 μM), HCT116 (IC50 = 3.51 ± 0.37 μM), HepG2 (IC50 = 1.50 ± 0.21 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Free	Amidation	① The Ⓧ (position: 6, 10, 18 and 22) in sequence indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ② Ⓧ (6) and Ⓧ (10), Ⓧ (18) and Ⓧ (22) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple, respectively.	L	No cytotoxicity information found in the reference	30789695	ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.	Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao	Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy	Stapled AMP					
DRAMP29019	IKLSKⓍTKDⓍLKKVLKGAIKGAIAVAKMV	IKLSKXTKDXLKKVLKGAIKGAIAVAKMV	IKLSPETKDNLKKVLKGAIKGAIAVAKMV	29	H-15	No entry found		Synthetic construct	Antimicrobial, Anticancer		α-helical (most likely)	No detailed structure description found.	Not found	"Function: Anticancer activity. 
Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays"	[Ref.30789695] Cancer cell lines: A549 (IC50 = 2.26 ± 0.24 μM), HCT116 (IC50 = 2.95 ± 0.25 μM), HepG2 (IC50 = 2.20 ± 0.27 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Free	Amidation	① The Ⓧ (position: 6 and 10) in sequence indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ② Ⓧ (6) and Ⓧ (10) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30789695	ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.	Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao	Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy	Stapled AMP					
DRAMP29020	IKLSPⓍTKKⓍLKKVLKGAIKGAIAVAKMV	IKLSPXTKKXLKKVLKGAIKGAIAVAKMV	IKLSPETKDNLKKVLKGAIKGAIAVAKMV	29	H-16	No entry found		Synthetic construct	Antimicrobial, Anticancer		α-helical (most likely)	No detailed structure description found.	Not found	"Function: Anticancer activity. 
Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays"	[Ref.30789695] Cancer cell lines: A549 (IC50 = 1.85 ± 0.31 μM), HCT116 (IC50 = 2.65 ± 0.35 μM), HepG2 (IC50 = 3.14 ± 0.46 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Free	Amidation	① The Ⓧ (position: 6 and 10) in sequence indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ② Ⓧ (6) and Ⓧ (10) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30789695	ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.	Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao	Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy	Stapled AMP					
DRAMP29021	IKLSKⓍTKKⓍLKKVLKGAIKGAIAVAKMV	IKLSKXTKKXLKKVLKGAIKGAIAVAKMV	IKLSPETKDNLKKVLKGAIKGAIAVAKMV	29	H-17	No entry found		Synthetic construct	Antimicrobial, Anticancer		α-helical (most likely)	No detailed structure description found.	Not found	"Function: Anticancer activity. 
Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays"	[Ref.30789695] Cancer cell lines: A549 (IC50 = 0.96 ± 0.33 μM), HCT116 (IC50 = 1.49 ± 0.45 μM), HepG2 (IC50 = 1.64 ± 0.47 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Free	Amidation	① The Ⓧ (position: 6 and 10) in sequence indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ② Ⓧ (6) and Ⓧ (10) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30789695	ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.	Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao	Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy	Stapled AMP					
DRAMP29022	IKLSKKTKKNLKKVLKGⓍIKGⓍIAVAKMV	IKLSKKTKKNLKKVLKGXIKGXIAVAKMV	IKLSPETKDNLKKVLKGAIKGAIAVAKMV	29	H-18	No entry found		Synthetic construct	Antimicrobial, Anticancer		α-helical (most likely)	No detailed structure description found.	Not found	"Function: Anticancer activity. 
Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays"	[Ref.30789695] Cancer cell lines: A549 (IC50 = 0.89 ± 0.21 μM), HCT116 (IC50 = 1.11 ± 0.21 μM), HepG2 (IC50 = 1.61 ± 0.21 μM)	[Ref.30789695] It has -1.5%, 3.8%, 12.8%, 18.2%, 13.8%, 30.2%, 65.2%, 90.8% and 83.5% hemolysis against fresh rabbit blood cells at 0.010, 0.25, 0.5, 1.0, 2.0, 5.0, 10.0, 20.0 and 25.0 μM.	Cyclic (Stapled)	Free	Amidation	① The Ⓧ (position: 18 and 22) in sequence indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ② Ⓧ (18) and Ⓧ (22) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30789695	ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.	Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao	Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy	Stapled AMP					
DRAMP29023	IKLSKETKKNLKKVLKGⓍIKGⓍIAVAKMV	IKLSKETKKNLKKVLKGXIKGXIAVAKMV	IKLSPETKDNLKKVLKGAIKGAIAVAKMV	29	H-19	No entry found		Synthetic construct	Antimicrobial, Anticancer		α-helical (most likely)	No detailed structure description found.	Not found	"Function: Anticancer activity. 
Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays"	[Ref.30789695] Cancer cell lines: A549 (IC50 = 1.00 ± 0.36 μM), HCT116 (IC50 = 1.78 ± 0.35 μM), HepG2 (IC50 = 1.64 ± 0.36 μM)	[Ref.30789695] It has 1.8%, 11.5%, 22.2%, 20.5%, 16.2%, 39.5%, 69.2%, 94.8% and 102.5% hemolysis against fresh rabbit blood cells at 0.010, 0.25, 0.5, 1.0, 2.0, 5.0, 10.0, 20.0 and 25.0 μM.	Cyclic (Stapled)	Free	Amidation	① The Ⓧ (position: 18 and 22) in sequence indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ② Ⓧ (18) and Ⓧ (22) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple.	L	No cytotoxicity information found in the reference	30789695	ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.	Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao	Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy	Stapled AMP					
DRAMP29024	IKLSPⓍTKKⓍLKKVLKGⓍIKGⓍIAVAKMV	IKLSPXTKKXLKKVLKGXIKGXIAVAKMV	IKLSPETKDNLKKVLKGAIKGAIAVAKMV	29	H-20	No entry found		Synthetic construct	Antimicrobial, Anticancer		α-helical (most likely)	No detailed structure description found.	Not found	"Function: Anticancer activity. 
Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays"	[Ref.30789695] Cancer cell lines: A549 (IC50 = 2.10 ± 0.32 μM), HCT116 (IC50 = 2.63 ± 0.35 μM), HepG2 (IC50 = 4.93 ± 0.53 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Free	Amidation	① The Ⓧ (position: 6, 10, 18 and 22) in sequence indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ② Ⓧ (6) and Ⓧ (10), Ⓧ (18) and Ⓧ (22) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple, respectively.	L	No cytotoxicity information found in the reference	30789695	ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.	Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao	Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy	Stapled AMP					
DRAMP29025	IKLSKⓍTKKⓍLKKVLKGⓍIKGⓍIAVAKMV	IKLSKXTKKXLKKVLKGXIKGXIAVAKMV	IKLSPETKDNLKKVLKGAIKGAIAVAKMV	29	H-21	No entry found		Synthetic construct	Antimicrobial, Anticancer		α-helical (most likely)	No detailed structure description found.	Not found	"Function: Anticancer activity. 
Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays"	[Ref.30789695] Cancer cell lines: A549 (IC50 = 3.26 ± 0.36 μM), HCT116 (IC50 = 3.05 ± 0.21 μM), HepG2 (IC50 = 1.50 ± 0.28 μM)	No hemolytic activity information found.	Cyclic (Stapled)	Free	Amidation	① The Ⓧ (position: 6, 10, 18 and 22) in sequence indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ② Ⓧ (6) and Ⓧ (10), Ⓧ (18) and Ⓧ (22) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple, respectively.	L	No cytotoxicity information found in the reference	30789695	ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.	Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao	Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy	Stapled AMP					
DRAMP29026	IKLSKETKKNLKKVLKGⓍIKGⓍIAVAKMV	IKLSKETKKNLKKVLKGXIKGXIAVAKMV	IKLSPETKDNLKKVLKGAIKGAIAVAKMV	29	H-57	No entry found		Synthetic construct	Antimicrobial, Anticancer		α-helical (most likely)	No detailed structure description found.	Not found	"Function: Anticancer activity. 
Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays"	[Ref.30789695] Cancer cell lines: A549 (IC50 = 1.10 ± 0.31 μM), HCT116 (IC50 = 1.65 ± 0.28 μM), HepG2 (IC50 = 2.71 ± 0.05 μM)	[Ref.30789695] It has -1.2%, 1.8%, 2.8%, 5.5%, 14.5%, 25.8%, 42.8%, 68.8% and 58.2% hemolysis against fresh rabbit blood cells at 0.010, 0.25, 0.5, 1.0, 2.0, 5.0, 10.0, 20.0 and 25.0 μM.	Cyclic (Stapled)	Free	Amidation	① The S (position: 4), T (position: 7) and N (position: 10) in sequence are linked with N-acetylglucosamine (GlcNAc), respectively. ② The Ⓧ (position: 18 and 22) indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ③ Ⓧ (18) and Ⓧ (22) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple, respectively.	L	No cytotoxicity information found in the reference	30789695	ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.	Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao	Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy	Stapled AMP					
DRAMP29027	IKLSKKTKKNLKKVLKGⓍIKGⓍIAVAKMV	IKLSKKTKKNLKKVLKGXIKGXIAVAKMV	IKLSPETKDNLKKVLKGAIKGAIAVAKMV	29	H-58	No entry found		Synthetic construct	Antimicrobial, Anticancer		α-helical (most likely)	No detailed structure description found.	Not found	"Function: Anticancer activity. 
Ref.30789695 does not include results of antimicrobial, hemolysis and other biological assays"	[Ref.30789695] Cancer cell lines: A549 (IC50 = 0.62 ± 0.12 μM), HCT116 (IC50 = 1.29 ± 0.08 μM), HepG2 (IC50 = 2.13 ± 0.07 μM)	[Ref.30789695] It has 0.8%, -0.5%, 0.8%, 8.8%, 13.2%, 20.8%, 37.8%, 61.8% and 54.5% hemolysis against fresh rabbit blood cells at 0.010, 0.25, 0.5, 1.0, 2.0, 5.0, 10.0, 20.0 and 25.0 μM.	Cyclic (Stapled)	Free	Amidation	① The S (position: 4), T (position: 7) and N (position: 10) in sequence are linked with N-acetylglucosamine (GlcNAc), respectively. ② The Ⓧ (position: 18 and 22) indicate (S)-N-Fmoc-2-(4'-pentenyl)alanine. ③ Ⓧ (18) and Ⓧ (22) are cross-linked by ring-closing metathesis through an oct-4-enyl hydrocarbon staple, respectively.	L	No cytotoxicity information found in the reference	30789695	ACS Chem Biol. 2019 Mar 15;14(3):516-525. doi: 10.1021/acschembio.9b00046. Epub 2019 Mar 1.	Yulei Li, Yihan Zhang, Minghao Wu, Qi Chang, Honggang Hu, Xia Zhao	Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy	Stapled AMP					
DRAMP29032	KLLKKAGKLLKKⓏGKLLKKⓍG	KLLKKAGKLLKKZGKLLKKXG	KLLKKAGKLLKKAGKLLKKAG	21	Stripe-based foldamer peptide 4	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-Helix content = 39% in 20 mM phosphate buffered saline (PBS) solution (pH 7.4), with 1% sodium dodecyl sulfate	[Ref.33369262] As shown in Figure 2, peptides 2, 3, 4 and 5 showed negative maxima at around 208 and 222nm, which suggests that they formed stable α-helical structures, similar to Stripe.	Not found	"Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.
It is a helical foldmer peptide based on ""Stripe"" (an AMP manually designed) by introducing a hydrocarbon stapling. The peptide showed weaker activity than Stripe."	[Ref.33369262] Gram-positive bacteria: Staphylococcus aureus NBRC13276 (MIC = 25 μM) ;##Gram-negative bacteria: Escherichia coli DH5α (MIC = 6.25 μM), Pseudomonas aeruginosa NBRC13275 (MIC = 50 μM), multidrug-resistant Pseudomonas aeruginosa ATCCBAA-2111 (MDRP) (MIC = 25 μM)	[Ref.33369262] It exhibits hemolysis at 0.78 μM agasint human red blood cells.	Cyclic (Stapled)	Free	Free	①The Ⓩ (position: 13) in sequence denotes (R)-(7-octenyl)alanine. ②The Ⓧ (position: 20) in sequence denotes (S)-(4-pentenyl)alanine. ③ Ⓩ (13) and Ⓧ (20) are cross-linked by side-stapling through a undec-4-enyl staple.	L	No cytotoxicity information found in the reference	33369262	Chempluschem. 2020 Dec;85(12):2731-2736. doi: 10.1002/cplu.202000749.	Motoharu Hirano, Chihiro Saito, Chihiro Goto, Hidetomo Yokoo, Ryuji Kawano, Takashi Misawa, Yosuke Demizu	Rational Design of Helix-Stabilized Antimicrobial Peptide Foldamers Containing α,α-Disubstituted Amino Acids or Side-Chain Stapling	Stapled AMP					
DRAMP29033	KLLKKⓏGKLLKKⓍGKLLKKAG	KLLKKZGKLLKKXGKLLKKAG	KLLKKAGKLLKKAGKLLKKAG	21	Stripe-based foldamer peptide 5	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		α-Helix content = 34% in 20 mM phosphate buffered saline (PBS) solution (pH 7.4), with 1% sodium dodecyl sulfate	[Ref.33369262] As shown in Figure 2, peptides 2, 3, 4 and 5 showed negative maxima at around 208 and 222nm, which suggests that they formed stable α-helical structures, similar to Stripe.	Not found	"Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.
It is a helical foldmer peptide based on ""Stripe"" (an AMP manually designed) by introducing a hydrocarbon stapling. The peptide showed weaker activity than Stripe."	[Ref.33369262] Gram-positive bacteria: Staphylococcus aureus NBRC13276 (MIC = 25 μM) ;##Gram-negative bacteria: Escherichia coli DH5α (MIC = 6.25 μM), Pseudomonas aeruginosa NBRC13275 (MIC = 12.5 μM), multidrug-resistant Pseudomonas aeruginosa ATCCBAA-2111 (MDRP) (MIC = 25 μM)	[Ref.33369262] It exhibits hemolysis at 1.56 μM agasint human red blood cells.	Cyclic (Stapled)	Free	Free	①The Ⓩ (position: 6) in sequence denotes (R)-(7-octenyl)alanine. ②The Ⓧ (position: 13) in sequence denotes (S)-(4-pentenyl)alanine. ③ Ⓩ (6) and Ⓧ (13) are cross-linked by side-stapling through a undec-4-enyl staple.	L	No cytotoxicity information found in the reference	33369262	Chempluschem. 2020 Dec;85(12):2731-2736. doi: 10.1002/cplu.202000749.	Motoharu Hirano, Chihiro Saito, Chihiro Goto, Hidetomo Yokoo, Ryuji Kawano, Takashi Misawa, Yosuke Demizu	Rational Design of Helix-Stabilized Antimicrobial Peptide Foldamers Containing α,α-Disubstituted Amino Acids or Side-Chain Stapling	Stapled AMP					
DRAMP29235	TIEEQAKTⓍLDKⓍNHEAEDLFYQⓍSLAⓍWN	TIEEQAKTXLDKXNHEAEDLFYQXSLAXWN	TIEEQAKTFLDKFNHEAEDLFYQSSLASWN	30	NYBSP-1	No entry found		Synthetic construct	Antimicrobial, Antiviral(SARS-CoV-2)		94% α-helical content in 10 mM phosphate-buffered saline (PBS)	No other descriptive information about the structure found in the literature	Not found	Mechanism of action:The stapled peptide designed based on the ACE2 helix, which is expected to bind to SARS-CoV-2 and prevent the binding of the virus to the ACE2 receptor and disrupt the infection.	[Ref.33310780]Virus:##SARS-CoV-2:Inhibition of infection in HT1080/ACE2 cells(IC50=4.1 ± 0.26 μM);Inhibition of infection in A549/ACE2 cells(IC50=2.2 ± 0.14 μM);Inhibition of virus-induced cytopathic effect (CPE) in Vero E6 cells(IC100=17.2 μM);##VSV-G:Inhibition of infection in HT1080/ACE2 cells(IC50>27.5 μM);Inhibition of infection in A549/ACE2 cells(IC50>27.5 μM).	No hemolytic activity information found.	Cyclic (Stapled)	Acylation	Amidation	①The Ⓧ (position: 9,13,24 and 28) in sequence indicate S-2-(4'-pentenyl) alanine.② Ⓧ(9) and Ⓧ(13), Ⓧ(24) and Ⓧ(28) are cross-linked by hydrocarbon stapling.	L	[Ref.33310780]HT1080/ACE2 cells:CC50>27.5 μM(Less than 10% toxicity at this dose);A549/ACE2 cells:CC50>27.5 μM(Less than 10% toxicity at this dose).	33310780	mBio. 2020 Dec 11;11(6):e02451-20.	Curreli F, Victor SMB, Ahmed S, Drelich A, Tong X, Tseng CK, Hillyer CD, Debnath AK.	Stapled Peptides Based on Human Angiotensin-Converting Enzyme 2 (ACE2) Potently Inhibit SARS-CoV-2 Infection In Vitro.	Stapled AMP					
DRAMP29236	TIEEQⓏKTFLDKⓍNHEAEDLⓏYQSSLAⓍWN	TIEEQZKTFLDKXNHEAEDLZYQSSLAXWN	TIEEQAKTFLDKFNHEAEDLFYQSSLASWN	30	NYBSP-2	No entry found		Synthetic construct	Antimicrobial, Antiviral(SARS-CoV-2)		61% α-helical content in 10 mM phosphate-buffered saline (PBS)	No other descriptive information about the structure found in the literature	Not found	Mechanism of action:The stapled peptide designed based on the ACE2 helix, which is expected to bind to SARS-CoV-2 and prevent the binding of the virus to the ACE2 receptor and disrupt the infection.	[Ref.33310780]Virus:##SARS-CoV-2:Inhibition of infection in HT1080/ACE2 cells(IC50=2.9 ± 0.27 μM);Inhibition of infection in A549/ACE2 cells(IC50=2.68 ± 0.14 μM);Inhibition of virus-induced cytopathic effect (CPE) in Vero E6 cells(IC100=33.5 μM);##VSV-G:Inhibition of infection in HT1080/ACE2 cells(IC50>26.8 μM);Inhibition of infection in A549/ACE2 cells(IC50>26.8 μM).	No hemolytic activity information found.	Cyclic (Stapled)	Acylation	Amidation	①The Ⓧ(position: 13 and 28) in sequence indicate S-2-(4'-pentenyl) alanine.②The Ⓩ(position: 6 and 21) indicate 2-(7'-octenyl) alanine in the R configuration.③ Ⓩ(6) and Ⓧ(13), Ⓩ(21) and Ⓧ(28) are cross-linked by hydrocarbon stapling.	L	[Ref.33310780]HT1080/ACE2 cells:CC50>26.8 μM(Less than 10% toxicity at this dose);A549/ACE2 cells:CC50>26.8 μM(Less than 10% toxicity at this dose).	33310780	mBio. 2020 Dec 11;11(6):e02451-20.	Curreli F, Victor SMB, Ahmed S, Drelich A, Tong X, Tseng CK, Hillyer CD, Debnath AK.	Stapled Peptides Based on Human Angiotensin-Converting Enzyme 2 (ACE2) Potently Inhibit SARS-CoV-2 Infection In Vitro.	Stapled AMP					
DRAMP29237	TIEEQAKTⓍLDKⓍNHEAEDLⓏYQSSLAⓍWN	TIEEQAKTXLDKXNHEAEDLZYQSSLAXWN	TIEEQAKTFLDKFNHEAEDLFYQSSLASWN	30	NYBSP-3	No entry found		Synthetic construct	Antimicrobial, Antiviral(SARS-CoV-2)		50% α-helical content in 10 mM phosphate-buffered saline (PBS)	No other descriptive information about the structure found in the literature	Not found	Mechanism of action:The stapled peptide designed based on the ACE2 helix, which is expected to bind to SARS-CoV-2 and prevent the binding of the virus to the ACE2 receptor and disrupt the infection.	[Ref.33310780]Virus:##SARS-CoV-2:Inhibition of infection in HT1080/ACE2 cells(IC50=12.9 ± 0.35 μM);Inhibition of infection in A549/ACE2 cells(IC50~25 μM);##VSV-G:Inhibition of infection in HT1080/ACE2 cells(IC50>27.6 μM);Inhibition of infection in A549/ACE2 cells(IC50>27.6 μM).	No hemolytic activity information found.	Cyclic (Stapled)	Acylation	Amidation	①The Ⓧ (position: 9,13 and 28) in sequence indicate S-2-(4'-pentenyl) alanine.②The Ⓩ (position: 21) indicates 2-(7'-octenyl) alanine in the R configuration.③ Ⓧ(9) and Ⓧ(13), Ⓩ(21) and Ⓧ(28) are cross-linked by hydrocarbon stapling.	L	[Ref.33310780]HT1080/ACE2 cells:CC50>27.6 μM(Less than 10% toxicity at this dose);A549/ACE2 cells:CC50>27.6 μM(Less than 10% toxicity at this dose).	33310780	mBio. 2020 Dec 11;11(6):e02451-20.	Curreli F, Victor SMB, Ahmed S, Drelich A, Tong X, Tseng CK, Hillyer CD, Debnath AK.	Stapled Peptides Based on Human Angiotensin-Converting Enzyme 2 (ACE2) Potently Inhibit SARS-CoV-2 Infection In Vitro.	Stapled AMP					
DRAMP29238	TIEEQⓏKTFLDKⓍNHEAEDLFYQⓍSLAⓍWN	TIEEQZKTFLDKXNHEAEDLFYQXSLAXWN	TIEEQAKTFLDKFNHEAEDLFYQSSLASWN	30	NYBSP-4	No entry found		Synthetic construct	Antimicrobial, Antiviral(SARS-CoV-2)		80% α-helical content in 10 mM phosphate-buffered saline (PBS)	No other descriptive information about the structure found in the literature	Not found	Mechanism of action:Proteolytic stability of NYBSP-4 in human plasma(half-life (T1/2) of NYBSP-4 was >289 min).The stapled peptide designed based on the ACE2 helix, which is expected to bind to SARS-CoV-2 and prevent the binding of the virus to the ACE2 receptor and disrupt the infection.	[Ref.33310780]Virus:##SARS-CoV-2:Inhibition of infection in HT1080/ACE2 cells(IC50=1.97 ± 0.14 μM);Inhibition of infection in A549/ACE2 cells(IC50=2.8 ± 0.08 μM);Inhibition of virus-induced cytopathic effect (CPE) in Vero E6 cells(IC100=33 μM);##VSV-G:Inhibition of infection in HT1080/ACE2 cells(IC50>26.6 μM);Inhibition of infection in A549/ACE2 cells(IC50>26.6 μM).	No hemolytic activity information found.	Cyclic (Stapled)	Acylation	Amidation	①The Ⓧ (position: 13,24 and 28) in sequence indicate S-2-(4'-pentenyl) alanine.②The Ⓩ (position: 6) indicates 2-(7'-octenyl) alanine in the R configuration.③  Ⓩ(6) and Ⓧ(13), Ⓧ(24) and Ⓧ(28) are cross-linked by hydrocarbon stapling.	L	[Ref.33310780]HT1080/ACE2 cells:CC50>26.6 μM(Less than 10% toxicity at this dose);A549/ACE2 cells:CC50>26.6 μM(Less than 10% toxicity at this dose).	33310780	mBio. 2020 Dec 11;11(6):e02451-20.	Curreli F, Victor SMB, Ahmed S, Drelich A, Tong X, Tseng CK, Hillyer CD, Debnath AK.	Stapled Peptides Based on Human Angiotensin-Converting Enzyme 2 (ACE2) Potently Inhibit SARS-CoV-2 Infection In Vitro.	Stapled AMP					
DRAMP29239	IEEQAKTFLDKFNHEⓀEDLⒺYQSSLASWNYNTNIT	IEEQAKTFLDKFNHEKEDLEYQSSLASWNYNTNIT	IEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNIT	35	hACE2(21-55)A36K-F40E	No entry found		Synthetic construct	Antimicrobial, Antiviral(SARS-CoV-2)		①52% α-helical content in 10 mM PBS at pH 7.4 with 30% TFE at 298 K.②6-13% α-helical content in 10 mM PBS at pH 7.4 and 298 K.	Low helicity for stapled hACE2 peptides in absence of TFE, with predictors of helicity averaging to 6-13% helical content. In the presence of TFE, α-helical structures can be observed for various synthetic hACE2 peptides with predictors averaging from 11 to 52% helical content	Not found	Mechanism of action:The stapled peptide inhibit the RBD-hACE2 complex formation, and hACE2 α1-helix-based peptidomimetics could potentially prevent SARS-CoV-2 from entering the human cells through hACE2 and thus inhibit subsequent viral replication. 	[Ref.33651072]Virus:SARS-CoV-2:inhibition of SARS-CoV-2 Spike protein-hACE2 complex formation(IC50=3.6 μM).	No hemolytic activity information found.	Cyclic (Stapled)	Free	Free	Ⓚ (16) and Ⓔ (20) are corss-linked by lactam stapling.	L	No cytotoxicity information found in the reference(s) presented	33651072	Chem Commun (Camb). 2021 Apr 4;57(26):3283-3286.	Maas MN , Hintzen JCJ , Löffler PMG , Mecinović J . 	Targeting SARS-CoV-2 spike protein by stapled hACE2 peptides.	Stapled AMP					
DRAMP29240	IEEQAKTFLDKⓀNHEⒺEDLFYQSSLASWNYNTNIT	IEEQAKTFLDKKNHEEEDLFYQSSLASWNYNTNIT	IEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNIT	35	hACE2(21-55)F32K-A36E	No entry found		Synthetic construct	Antimicrobial, Antiviral(SARS-CoV-2)		①6-13% α-helical content in 10 mM PBS at pH 7.4 and 298 K.②11-52% α-helical content in 10 mM PBS at pH 7.4 with 30% TFE at 298 K.	Low helicity for stapled hACE2 peptides in absence of TFE, with predictors of helicity averaging to 6-13% helical content. In the presence of TFE, α-helical structures can be observed for various synthetic hACE2 peptides with predictors averaging from 11 to 52% helical content	Not found	Mechanism of action:The stapled peptide inhibit the RBD-hACE2 complex formation, and hACE2 α1-helix-based peptidomimetics could potentially prevent SARS-CoV-2 from entering the human cells through hACE2 and thus inhibit subsequent viral replication. 	[Ref.33651072]Virus:SARS-CoV-2:inhibition of SARS-CoV-2 Spike protein-hACE2 complex formation(IC50=28.4 μM).	No hemolytic activity information found.	Cyclic (Stapled)	Free	Free	Ⓚ (12) and Ⓔ (16) are corss-linked by lactam stapling.	L	No cytotoxicity information found in the reference(s) presented	33651072	Chem Commun (Camb). 2021 Apr 4;57(26):3283-3286.	Maas MN , Hintzen JCJ , Löffler PMG , Mecinović J . 	Targeting SARS-CoV-2 spike protein by stapled hACE2 peptides.	Stapled AMP					
DRAMP29241	IEEQAKTⓀLDKⒺNHEAEDLFYQSSLASWNYNTNIT	IEEQAKTKLDKENHEAEDLFYQSSLASWNYNTNIT	IEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNIT	35	hACE2(21-55)F28K-F32E	No entry found		Synthetic construct	Antimicrobial, Antiviral(SARS-CoV-2)		①6-13% α-helical content in 10 mM PBS at pH 7.4 and 298 K.②11-52% α-helical content in 10 mM PBS at pH 7.4 with 30% TFE at 298 K.	Low helicity for stapled hACE2 peptides in absence of TFE, with predictors of helicity averaging to 6-13% helical content. In the presence of TFE, α-helical structures can be observed for various synthetic hACE2 peptides with predictors averaging from 11 to 52% helical content	Not found	Mechanism of action:The stapled peptide inhibit the RBD-hACE2 complex formation, and hACE2 α1-helix-based peptidomimetics could potentially prevent SARS-CoV-2 from entering the human cells through hACE2 and thus inhibit subsequent viral replication. 	[Ref.33651072]Virus:SARS-CoV-2:inhibition of SARS-CoV-2 Spike protein-hACE2 complex formation(IC50=46.8 μM).	No hemolytic activity information found.	Cyclic (Stapled)	Free	Free	Ⓚ (8) and Ⓔ (12) are corss-linked by lactam stapling.	L	No cytotoxicity information found in the reference(s) presented	33651072	Chem Commun (Camb). 2021 Apr 4;57(26):3283-3286.	Maas MN , Hintzen JCJ , Löffler PMG , Mecinović J . 	Targeting SARS-CoV-2 spike protein by stapled hACE2 peptides.	Stapled AMP					
DRAMP29334	FFGSVLⓀLIPⓀIL	FFGSVLKLIPKIL	FFGSVLKLIPKIL	13	LS-Temporin-Pta	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=4 μg/mL), Staphylococcus aureus BNCC 186335(MIC=4 μg/mL), Staphylococcus aureus(MRSA)(MIC=4 μg/mL) ;##Gram-negative bacteria:P. aeruginosa(MIC>128 μg/mL), E. coli(MIC=64 μg/mL), Cephalosporin-resistant E. coli(MIC=128 μg/mL).	[Ref.34968054]MHC=32 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 7 and 11) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (7) and Ⓚ (11) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	"[Ref.34968054]Human embryonic kidney HEK293T cells: LC90=64	µg/ml."	34968054	J Med Chem. 2022 Jan 13;65(1):579-591. 	Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J. 	Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.	Stapled AMP					
DRAMP29335	GFKDLLKGAAⓀALVⓀTVLF	GFKDLLKGAAKALVKTVLF	GFKDLLKGAAKALVKTVLF	19	LS-Ascaphin-8 P1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=4 μg/mL), Staphylococcus aureus BNCC 186335(MIC=2 μg/mL),  Staphylococcus aureus(MRSA)(MIC=8 μg/mL) ;##Gram-negative bacteria:P. aeruginosa(MIC=128 μg/mL), E. coli(MIC=8 μg/mL), Cephalosporin-resistant E. coli(MIC=16 μg/mL).	[Ref.34968054]MHC=32 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 11 and 15) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (11) and Ⓚ (15) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	[Ref.34968054]Human embryonic kidney HEK293T cells: LC90=128 µg/ml.	34968054	J Med Chem. 2022 Jan 13;65(1):579-591. 	Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J. 	Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.	Stapled AMP					
DRAMP29336	GFKDLLⓀGAAⓀALVKTVLF	GFKDLLKGAAKALVKTVLF	GFKDLLKGAAKALVKTVLF	19	LS-Ascaphin-8 P2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=8μg/mL), Staphylococcus aureus BNCC 186335(MIC=2 μg/mL),  Staphylococcus aureus(MRSA)(MIC=8 μg/mL) ;##Gram-negative bacteria:P. aeruginosa(MIC=32 μg/mL), E. coli(MIC=16 μg/mL), Cephalosporin-resistant E. coli(MIC=32 μg/mL).	[Ref.34968054]MHC=32 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 7 and 11) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (7) and Ⓚ (11) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	[Ref.34968054]Human embryonic kidney HEK293T cells: LC90=128 µg/ml.	34968054	J Med Chem. 2022 Jan 13;65(1):579-591. 	Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J. 	Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.	Stapled AMP					
DRAMP29337	GFⓀDLLⓀGAAKALVKTVLF	GFKDLLKGAAKALVKTVLF	GFKDLLKGAAKALVKTVLF	19	LS-Ascaphin-8 P3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=16 μg/mL), Staphylococcus aureus BNCC 186335(MIC=4 μg/mL),  Staphylococcus aureus(MRSA)(MIC=16 μg/mL) ;##Gram-negative bacteria:P. aeruginosa(MIC=128 μg/mL), E. coli(MIC=16 μg/mL), Cephalosporin-resistant E. coli(MIC=32 μg/mL).	[Ref.34968054]MHC=32 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 3 and 7) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (3) and Ⓚ (7) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	[Ref.34968054]Human embryonic kidney HEK293T cells: LC50=100 µg/ml.	34968054	J Med Chem. 2022 Jan 13;65(1):579-591. 	Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J. 	Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.	Stapled AMP					
DRAMP29338	FFGKVLⓀLIRⓀIF	FFGKVLKLIRKIF	FFGKVLKLIRKIF	13	LS-DASamP1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=8μg/mL), Staphylococcus aureus BNCC 186335(MIC=16 μg/mL),  Staphylococcus aureus(MRSA)(MIC=32 μg/mL) ;##Gram-negative bacteria:P. aeruginosa(MIC>128 μg/mL), E. coli(MIC=128 μg/mL), Cephalosporin-resistant E. coli(MIC=128 μg/mL).	[Ref.34968054]MHC=16 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 7 and 11) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (7) and Ⓚ (11) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	[Ref.34968054]Human embryonic kidney HEK293T cells: LC50=128 µg/ml.	34968054	J Med Chem. 2022 Jan 13;65(1):579-591. 	Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J. 	Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.	Stapled AMP					
DRAMP29339	PKILNⓀILGⓀILRLAAAFK	PKILNKILGKILRLAAAFK	PKILNKILGKILRLAAAFK	19	LS-PaDBS1R1-1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=2 μg/mL), Staphylococcus aureus BNCC 186335(MIC=2 μg/mL),  Staphylococcus aureus(MRSA)(MIC=4 μg/mL) ;##Gram-negative bacteria:P. aeruginosa(MIC=16 μg/mL), E. coli(MIC=4 μg/mL), Cephalosporin-resistant E. coli(MIC=8 μg/mL).	[Ref.34968054]MHC=16 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 6 and 10) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (6) and Ⓚ (10) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	[Ref.34968054]Human embryonic kidney HEK293T cells: LC90=128 µg/ml.	34968054	J Med Chem. 2022 Jan 13;65(1):579-591. 	Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J. 	Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.	Stapled AMP					
DRAMP29340	PⓀILNⓀILGKILRLAAAFK	PKILNKILGKILRLAAAFK	PKILNKILGKILRLAAAFK	19	LS-PaDBS1R1-2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=4 μg/mL), Staphylococcus aureus BNCC 186335(MIC=4 μg/mL),  Staphylococcus aureus(MRSA)(MIC=4 μg/mL) ;##Gram-negative bacteria:P. aeruginosa(MIC=128 μg/mL), E. coli(MIC=16 μg/mL), Cephalosporin-resistant E. coli(MIC=16 μg/mL).	[Ref.34968054]MHC=16 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 2 and 6) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (2) and Ⓚ (6) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	[Ref.34968054]Human embryonic kidney HEK293T cells: LC50=100 µg/ml.	34968054	J Med Chem. 2022 Jan 13;65(1):579-591. 	Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J. 	Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.	Stapled AMP					
DRAMP29341	VKRFKⓀFFRⓀFKKFV	VKRFKKFFRKFKKFV	VKRFKKFFRKFKKFV	15	LS-Cathelicidin-BF-15-a1-1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=4μg/mL), Staphylococcus aureus BNCC 186335(MIC=2 μg/mL),  Staphylococcus aureus(MRSA)(MIC=2 μg/mL) ;##Gram-negative bacteria:P. aeruginosa(MIC=16 μg/mL), E. coli(MIC=2 μg/mL), Cephalosporin-resistant E. coli(MIC=8 μg/mL).	[Ref.34968054]MHC>128 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 6 and 10) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (6) and Ⓚ (10) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	"[Ref.34968054]Human embryonic kidney HEK293T cells: LC50=64	µg/ml."	34968054	J Med Chem. 2022 Jan 13;65(1):579-591. 	Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J. 	Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.	Stapled AMP					
DRAMP29342	VⓀRFKⓀFFRKFKKFV	VKRFKKFFRKFKKFV	VKRFKKFFRKFKKFV	15	LS-Cathelicidin-BF-15-a1-2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=16μg/mL), Staphylococcus aureus BNCC 186335(MIC=2 μg/mL),  Staphylococcus aureus(MRSA)(MIC=4 μg/mL) ;##Gram-negative bacteria:P. aeruginosa(MIC=8 μg/mL), E. coli(MIC=4 μg/mL), Cephalosporin-resistant E. coli(MIC=16 μg/mL).	[Ref.34968054]MHC>128 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 2 and 6) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (2) and Ⓚ (6) are cross-linked by a (E)-but-2-enyl spacer employing the N-alkylation reaction.	L	[Ref.34968054]Human embryonic kidney HEK293T cells: LC50=100 µg/ml.	34968054	J Med Chem. 2022 Jan 13;65(1):579-591. 	Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J. 	Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.	Stapled AMP					
DRAMP29343	VKRFKⓀFFRⓀFKKFV	VKRFKKFFRKFKKFV	VKRFKKFFRKFKKFV	15	LS-Cathelicidin-BF-15-a1-3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=4μg/mL), Staphylococcus aureus BNCC 186335(MIC=2 μg/mL),  Staphylococcus aureus(MRSA)(MIC=4 μg/mL) ;##Gram-negative bacteria:P. aeruginosa(MIC=8 μg/mL), E. coli(MIC=4 μg/mL), Cephalosporin-resistant E. coli(MIC=8 μg/mL).	[Ref.34968054]MHC=32 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 6 and 10) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (6) and Ⓚ (10) are cross-linked by 1,2-bismethylenebenzene via N-alkylation reaction.	L	[Ref.34968054]Human embryonic kidney HEK293T cells: LC90=128 µg/ml.	34968054	J Med Chem. 2022 Jan 13;65(1):579-591. 	Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J. 	Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.	Stapled AMP					
DRAMP29344	VⓀRFKⓀFFRKFKKFV	VKRFKKFFRKFKKFV	VKRFKKFFRKFKKFV	15	LS-Cathelicidin-BF-15-a1-4	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=16 μg/mL), Staphylococcus aureus BNCC 186335(MIC=4 μg/mL),  Staphylococcus aureus(MRSA)(MIC=8 μg/mL) ;##Gram-negative bacteria:P. aeruginosa(MIC=16 μg/mL), E. coli(MIC=8 μg/mL), Cephalosporin-resistant E. coli(MIC=16 μg/mL).	[Ref.34968054]MHC>128 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 2 and 6) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (2) and Ⓚ (6) are cross-linked by 1,2-bismethylenebenzene via N-alkylation reaction.	L	[Ref.34968054]Human embryonic kidney HEK293T cells: LC50=128 µg/ml.	34968054	J Med Chem. 2022 Jan 13;65(1):579-591. 	Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J. 	Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.	Stapled AMP					
DRAMP29345	VKRFKⓀFFRⓀFKKFV	VKRFKKFFRKFKKFV	VKRFKKFFRKFKKFV	15	LS-Cathelicidin-BF-15-a1-5	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=2 μg/mL), Staphylococcus aureus BNCC 186335(MIC=2 μg/mL),  Staphylococcus aureus(MRSA)(MIC=4 μg/mL) ;##Gram-negative bacteria:P. aeruginosa(MIC=16 μg/mL), E. coli(MIC=2 μg/mL), Cephalosporin-resistant E. coli(MIC=16 μg/mL).	[Ref.34968054]MHC>128 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 6 and 10) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (6) and Ⓚ (10) are cross-linked by a but-2-ynyl spacer employing the N-alkylation reaction.	L	[Ref.34968054]Human embryonic kidney HEK293T cells: LC90=128 µg/ml.	34968054	J Med Chem. 2022 Jan 13;65(1):579-591. 	Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J. 	Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.	Stapled AMP					
DRAMP29346	VⓀRFKⓀFFRKFKKFV	VKRFKKFFRKFKKFV	VKRFKKFFRKFKKFV	15	LS-Cathelicidin-BF-15-a1-6	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.34968054]Gram-positive bacteria:Listeria monocytogenes(MIC=8μg/mL), Staphylococcus aureus BNCC 186335(MIC=4 μg/mL),  Staphylococcus aureus(MRSA)(MIC=8 μg/mL) ;##Gram-negative bacteria:P. aeruginosa(MIC=16 μg/mL), E. coli(MIC=4 μg/mL), Cephalosporin-resistant E. coli(MIC=16 μg/mL).	[Ref.34968054]MHC>128 μg/mL. The MHC is the minimum peptide concentration that caused 10% hemolysis of hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓚ (position: 2 and 6) in sequence indicates Nε-o-Ns-Nα-Fmoc-lysine before stapling. ②Ⓚ (2) and Ⓚ (6) are cross-linked by a but-2-ynyl spacer employing the N-alkylation reaction.	L	[Ref.34968054]Human embryonic kidney HEK293T cells: LC50=100 µg/ml.	34968054	J Med Chem. 2022 Jan 13;65(1):579-591. 	Hu Y, Li H, Qu R, He T, Tang X, Chen W, Li L, Bai H, Li C, Wang W, Fu G, Luo G, Xia X, Zhang J. 	Lysine Stapling Screening Provides Stable and Low Toxic Cationic Antimicrobial Peptides Combating Multidrug-Resistant Bacteria In Vitro and In Vivo.	Stapled AMP					
DRAMP29347	FIIIKKSGGLFKKKAGAⓧKKKⓧIKK	FIIIKKSGGLFKKKAGAKKKKKIKK	FIHHIIGGLFSAGKAIHRLIRRRRR	25	TP4-1	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.36877022]Gram-positive bacteria: Staphylococcus aureus(MIC>25 μM; MBC>25 μM);##Gram-negative bacteria:Acinetobacter baumannii(MIC>25 μM; MBC>25 μM).	[Ref.36877022]It shows 15% hemolysis against human red blood cells at the concentration greater than 100 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 18 and 22) in sequence indicates Fmoc-(R)-2-(4-pentenyl) alanine. ②Ⓧ (18) and Ⓧ (22) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	36877022	Microbiol Spectr. 2023 Mar 6;11(2):e0385322.	Yeh JC, Hazam PK, Hsieh CY, Hsu PH, Lin WC, Chen YR, Li CC, Chen JY. 	Rational Design of Stapled Antimicrobial Peptides to Enhance Stability and In Vivo Potency against Polymicrobial Sepsis. 	Stapled AMP					
DRAMP29348	FIIIKKSGGLⓧKKKⓧGAⓧKKKⓧIKK	FIIIKKSGGLKKKKKGAKKKKKIKK	FIHHIIGGLFSAGKAIHRLIRRRRR	25	TP4-2	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.36877022]Gram-positive bacteria: Staphylococcus aureus(MIC>25 μM; MBC>25 μM);##Gram-negative bacteria:Acinetobacter baumannii(MIC=12.5 μM; MBC=12.5 μM).	[Ref.36877022]It shows 15% hemolysis against human red blood cells at the concentration greater than 100 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 11, 15, 18 and 22) in sequence indicates Fmoc-(R)-2-(4-pentenyl) alanine. ②Ⓧ (11) and Ⓧ (15), Ⓧ (18) and Ⓧ (22) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	36877022	Microbiol Spectr. 2023 Mar 6;11(2):e0385322.	Yeh JC, Hazam PK, Hsieh CY, Hsu PH, Lin WC, Chen YR, Li CC, Chen JY. 	Rational Design of Stapled Antimicrobial Peptides to Enhance Stability and In Vivo Potency against Polymicrobial Sepsis. 	Stapled AMP					
DRAMP29349	FIIⓧKKSⓧGLFKKKAGAⓧKKKⓧIKK	FIIKKKSKGLFKKKAGAKKKKKIKK	FIHHIIGGLFSAGKAIHRLIRRRRR	25	TP4-3	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	TP4-3 retained its activity against A. baumannii in the presence of 50% human serum(MIC=3.13 μM; MBC=3.13 μM).	[Ref.36877022]Gram-positive bacteria: Staphylococcus aureus(MIC>25 μM; MBC>25 μM);##Gram-negative bacteria:Acinetobacter baumannii(MIC=3.13 μM; MBC=3.13 μM).	[Ref.36877022]It shows 15% hemolysis against human red blood cells at the concentration greater than 100 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 4, 8, 18 and 22) in sequence indicates Fmoc-(R)-2-(4-pentenyl) alanine.②Ⓧ (4) and Ⓧ (8), Ⓧ (18) and Ⓧ (22) are cross-linked by hydrocarbon stapling respectively.	L	[Ref.36877022]TP4-3 showed <10% cell death at 3.13 μM for HaCaT cells, 3.13 μM for HK-2 cells, and 12.5 μM for RAW 264.7 macrophage cells. 	36877022	Microbiol Spectr. 2023 Mar 6;11(2):e0385322.	Yeh JC, Hazam PK, Hsieh CY, Hsu PH, Lin WC, Chen YR, Li CC, Chen JY. 	Rational Design of Stapled Antimicrobial Peptides to Enhance Stability and In Vivo Potency against Polymicrobial Sepsis. 	Stapled AMP					
DRAMP29350	FIIⓧKKSⓧGLⓧKKKⓧGAⓧKKKⓧIKK	FIIKKKSKGLKKKKKGAKKKKKIKK	FIHHIIGGLFSAGKAIHRLIRRRRR	25	TP4-4	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram+, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-positive and Gram-negative bacteria.	[Ref.36877022]Gram-positive bacteria: Staphylococcus aureus(MIC>25 μM; MBC>25 μM);##Gram-negative bacteria:Acinetobacter baumannii(MIC=12.5 μM; MBC=12.5 μM).	[Ref.36877022]It shows 15% hemolysis against human red blood cells at the concentration greater than 100 μM.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 4, 8, 11, 15, 18 and 22) in sequence indicates Fmoc-(R)-2-(4-pentenyl) alanine. ②Ⓧ (4) and Ⓧ (8), Ⓧ (11) and Ⓧ (15),  Ⓧ (18) and Ⓧ (22) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	36877022	Microbiol Spectr. 2023 Mar 6;11(2):e0385322.	Yeh JC, Hazam PK, Hsieh CY, Hsu PH, Lin WC, Chen YR, Li CC, Chen JY. 	Rational Design of Stapled Antimicrobial Peptides to Enhance Stability and In Vivo Potency against Polymicrobial Sepsis. 	Stapled AMP					
DRAMP29351	ⓧLFDⓧIKKIAESF	XLFDXIKKIAESF	GLFDIIKKIAESF	13	SAU-1	No entry found		Synthetic construct	Antimicrobial, Antifungal		60.2% α-helix in a solution of phosphate buffer and trifluoroethanol (7:3) and reached a concentration of 50 μM. 	No other descriptive information about the structure found in the literature	Not available	Function: Antifungal.	[Ref.34439006]Fungi: C. albicans SC5314(MIC>64 μg/mL), C. albicans 901(MIC=32 μg/mL),  C. albicans 904(MIC>128 μg/mL), C. tropicalis ATCC 20026 (MIC>128 μg/mL),  C. tropicalis 895 (MIC>128 μg/mL),  C. glabrata ATCC 1182 (MIC>128 μg/mL),  C. glabrata 896 (MIC>128 μg/mL),  C. auris 918 (MIC>128 μg/mL),  C. auris 919 (MIC>128 μg/mL),  C. krusei ATCC 2340 (MIC>128 μg/mL),  C. parapsilosis ATCC 22010(MIC=128 μg/mL).	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 1 and 5) in sequence indicates S5 (2-amino-2-methylhept-6-enoic acid). ②Ⓧ (1) and Ⓧ (5) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	34439006	Antibiotics (Basel). 2021 Aug 9;10(8):956. 	Zheng M, Wang R, Chen S, Zou Y, Yan L, Zhao L, Li X.	Design, Synthesis and Antifungal Activity of Stapled Aurein1.2 Peptides. 	Stapled AMP					
DRAMP29352	GⓧFDIⓧKKIAESF	GXFDIXKKIAESF	GLFDIIKKIAESF	13	SAU-2	No entry found		Synthetic construct	Antimicrobial, Antifungal		52.5% α-helix in a solution of phosphate buffer and trifluoroethanol (7:3) and reached a concentration of 50 μM. 	No other descriptive information about the structure found in the literature	Not available	Function: Antifungal.	[Ref.34439006]Fungi: C. albicans SC5314(MIC>64 μg/mL), C. albicans 901(MIC>128 μg/mL),  C. albicans 904(MIC>128 μg/mL), C. tropicalis ATCC 20026 (MIC>128 μg/mL),  C. tropicalis 895 (MIC=16 μg/mL),  C. glabrata ATCC 1182 (MIC>128 μg/mL),  C. glabrata 896 (MIC=128 μg/mL),  C. auris 918 (MIC>128 μg/mL),  C. auris 919 (MIC>128 μg/mL),  C. krusei ATCC 2340 (MIC>128 μg/mL),  C. parapsilosis ATCC 22010(MIC>128 μg/mL).	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates S5 (2-amino-2-methylhept-6-enoic acid). ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	34439006	Antibiotics (Basel). 2021 Aug 9;10(8):956. 	Zheng M, Wang R, Chen S, Zou Y, Yan L, Zhao L, Li X.	Design, Synthesis and Antifungal Activity of Stapled Aurein1.2 Peptides. 	Stapled AMP					
DRAMP29353	GLⓧDIIⓧKIAESF	GLXDIIXKIAESF	GLFDIIKKIAESF	13	SAU-3	No entry found		Synthetic construct	Antimicrobial, Antifungal		72.3% α-helix in a solution of phosphate buffer and trifluoroethanol (7:3) and reached a concentration of 50 μM. 	No other descriptive information about the structure found in the literature	Not available	Function: Antifungal.	[Ref.34439006]Fungi: C. albicans SC5314(MIC>64 μg/mL), C. albicans 901(MIC>128 μg/mL),  C. albicans 904(MIC>128 μg/mL), C. tropicalis ATCC 20026 (MIC>128 μg/mL),  C. tropicalis 895 (MIC>128 μg/mL),  C. glabrata ATCC 1182 (MIC>128 μg/mL),  C. glabrata 896 (MIC>128 μg/mL),  C. auris 918 (MIC>128 μg/mL),  C. auris 919 (MIC>128 μg/mL),  C. krusei ATCC 2340 (MIC>128 μg/mL),  C. parapsilosis ATCC 22010(MIC>128 μg/mL).	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 3 and 7) in sequence indicates S5 (2-amino-2-methylhept-6-enoic acid). ②Ⓧ (3) and Ⓧ (7) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	34439006	Antibiotics (Basel). 2021 Aug 9;10(8):956. 	Zheng M, Wang R, Chen S, Zou Y, Yan L, Zhao L, Li X.	Design, Synthesis and Antifungal Activity of Stapled Aurein1.2 Peptides. 	Stapled AMP					
DRAMP29354	GLFⓧIIKⓧIAESF	GLFXIIKXIAESF	GLFDIIKKIAESF	13	SAU-4	No entry found		Synthetic construct	Antimicrobial, Antifungal		73.5% α-helix in a solution of phosphate buffer and trifluoroethanol (7:3) and reached a concentration of 50 μM. 	No other descriptive information about the structure found in the literature	Not available	Function: Antifungal.	[Ref.34439006]Fungi: C. albicans SC5314(MIC>64 μg/mL), C. albicans 901(MIC>128 μg/mL),  C. albicans 904(MIC>128 μg/mL), C. tropicalis ATCC 20026 (MIC>128 μg/mL),  C. tropicalis 895 (MIC>128 μg/mL),  C. glabrata ATCC 1182 (MIC>128 μg/mL),  C. glabrata 896 (MIC>128 μg/mL),  C. auris 918 (MIC>128 μg/mL),  C. auris 919 (MIC>128 μg/mL),  C. krusei ATCC 2340 (MIC>128 μg/mL),  C. parapsilosis ATCC 22010(MIC>128 μg/mL).	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 4 and 8) in sequence indicates S5 (2-amino-2-methylhept-6-enoic acid). ②Ⓧ (4) and Ⓧ (8) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	34439006	Antibiotics (Basel). 2021 Aug 9;10(8):956. 	Zheng M, Wang R, Chen S, Zou Y, Yan L, Zhao L, Li X.	Design, Synthesis and Antifungal Activity of Stapled Aurein1.2 Peptides. 	Stapled AMP					
DRAMP29355	GLFDⓧIKKⓧAESF	GLFDXIKKXAESF	GLFDIIKKIAESF	13	SAU-5	No entry found		Synthetic construct	Antimicrobial, Antifungal		61.0% α-helix in a solution of phosphate buffer and trifluoroethanol (7:3) and reached a concentration of 50 μM. 	No other descriptive information about the structure found in the literature	Not available	Function: Antifungal.	[Ref.34439006]Fungi: C. albicans SC5314(MIC=16 μg/mL), C. albicans 901(MIC>128 μg/mL),  C. albicans 904(MIC>128 μg/mL), C. tropicalis ATCC 20026 (MIC>128 μg/mL),  C. tropicalis 895 (MIC=16 μg/mL),  C. glabrata ATCC 1182 (MIC>128 μg/mL),  C. glabrata 896 (MIC>128 μg/mL),  C. auris 918 (MIC>128 μg/mL),  C. auris 919 (MIC>128 μg/mL),  C. krusei ATCC 2340 (MIC>128 μg/mL),  C. parapsilosis ATCC 22010(MIC>128 μg/mL).	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 5 and 9) in sequence indicates S5 (2-amino-2-methylhept-6-enoic acid). ②Ⓧ (5) and Ⓧ (9) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	34439006	Antibiotics (Basel). 2021 Aug 9;10(8):956. 	Zheng M, Wang R, Chen S, Zou Y, Yan L, Zhao L, Li X.	Design, Synthesis and Antifungal Activity of Stapled Aurein1.3 Peptides.	Stapled AMP					
DRAMP29356	GLFDIⓧKKIⓧESF	GLFDIXKKIXESF	GLFDIIKKIAESF	13	SAU-6	No entry found		Synthetic construct	Antimicrobial, Antifungal		49.9% α-helix in a solution of phosphate buffer and trifluoroethanol (7:3) and reached a concentration of 50 μM. 	No other descriptive information about the structure found in the literature	Not available	Function: Antifungal.	[Ref.34439006]Fungi: C. albicans SC5314(MIC>64 μg/mL), C. albicans 901(MIC>128 μg/mL),  C. albicans 904(MIC>128 μg/mL), C. tropicalis ATCC 20026 (MIC>128 μg/mL),  C. tropicalis 895 (MIC>128 μg/mL),  C. glabrata ATCC 1182 (MIC>128 μg/mL),  C. glabrata 896 (MIC>128 μg/mL),  C. auris 918 (MIC>128 μg/mL),  C. auris 919 (MIC>128 μg/mL),  C. krusei ATCC 2340 (MIC>128 μg/mL),  C. parapsilosis ATCC 22010(MIC>128 μg/mL).	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 6 and 10) in sequence indicates S5 (2-amino-2-methylhept-6-enoic acid). ②Ⓧ (6) and Ⓧ (10) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	34439006	Antibiotics (Basel). 2021 Aug 9;10(8):956. 	Zheng M, Wang R, Chen S, Zou Y, Yan L, Zhao L, Li X.	Design, Synthesis and Antifungal Activity of Stapled Aurein1.2 Peptides. 	Stapled AMP					
DRAMP29357	GLFDIIⓧKIAⓧSF	GLFDIIXKIAXSF	GLFDIIKKIAESF	13	SAU-7	No entry found		Synthetic construct	Antimicrobial, Antifungal		65.0% α-helix in a solution of phosphate buffer and trifluoroethanol (7:3) and reached a concentration of 50 μM. 	No other descriptive information about the structure found in the literature	Not available	Function: Antifungal.	[Ref.34439006]Fungi: C. albicans SC5314(MIC>64 μg/mL), C. albicans 901(MIC>128 μg/mL),  C. albicans 904(MIC>128 μg/mL), C. tropicalis ATCC 20026 (MIC>128 μg/mL),  C. tropicalis 895 (MIC>128 μg/mL),  C. glabrata ATCC 1182 (MIC>128 μg/mL),  C. glabrata 896 (MIC>128 μg/mL),  C. auris 918 (MIC>128 μg/mL),  C. auris 919 (MIC>128 μg/mL),  C. krusei ATCC 2340 (MIC>128 μg/mL),  C. parapsilosis ATCC 22010(MIC>128 μg/mL).	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 7 and 11) in sequence indicates S5 (2-amino-2-methylhept-6-enoic acid). ②Ⓧ (7) and Ⓧ (11) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	34439006	Antibiotics (Basel). 2021 Aug 9;10(8):956. 	Zheng M, Wang R, Chen S, Zou Y, Yan L, Zhao L, Li X.	Design, Synthesis and Antifungal Activity of Stapled Aurein1.2 Peptides. 	Stapled AMP					
DRAMP29358	GLFDIIKⓧIAEⓧF	GLFDIIKXIAEXF	GLFDIIKKIAESF	13	SAU-8	No entry found		Synthetic construct	Antimicrobial, Antifungal		83.1% α-helix in a solution of phosphate buffer and trifluoroethanol (7:3) and reached a concentration of 50 μM. 	No other descriptive information about the structure found in the literature	Not available	Function: Antifungal.	[Ref.34439006]Fungi: C. albicans SC5314(MIC>64 μg/mL), C. albicans 901(MIC>128 μg/mL),  C. albicans 904(MIC>128 μg/mL), C. tropicalis ATCC 20026 (MIC>128 μg/mL),  C. tropicalis 895 (MIC>128 μg/mL),  C. glabrata ATCC 1182 (MIC>128 μg/mL),  C. glabrata 896 (MIC>128 μg/mL),  C. auris 918 (MIC>128 μg/mL),  C. auris 919 (MIC>128 μg/mL),  C. krusei ATCC 2340 (MIC>128 μg/mL),  C. parapsilosis ATCC 22010(MIC>128 μg/mL).	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 8 and 12) in sequence indicates S5 (2-amino-2-methylhept-6-enoic acid). ②Ⓧ (8) and Ⓧ (12) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	34439006	Antibiotics (Basel). 2021 Aug 9;10(8):956. 	Zheng M, Wang R, Chen S, Zou Y, Yan L, Zhao L, Li X.	Design, Synthesis and Antifungal Activity of Stapled Aurein1.2 Peptides. 	Stapled AMP					
DRAMP29359	GLFDIIKKⓧAESⓧ	GLFDIIKKXAESX	GLFDIIKKIAESF	13	SAU-9	No entry found		Synthetic construct	Antimicrobial, Antifungal		67.8% α-helix in a solution of phosphate buffer and trifluoroethanol (7:3) and reached a concentration of 50 μM. 	No other descriptive information about the structure found in the literature	Not available	Function: Antifungal.	[Ref.34439006]Fungi: C. albicans SC5314(MIC>64 μg/mL), C. albicans 901(MIC>128 μg/mL),  C. albicans 904(MIC>128 μg/mL), C. tropicalis ATCC 20026 (MIC>128 μg/mL),  C. tropicalis 895 (MIC=32 μg/mL),  C. glabrata ATCC 1182 (MIC>128 μg/mL),  C. glabrata 896 (MIC>128 μg/mL),  C. auris 918 (MIC>128 μg/mL),  C. auris 919 (MIC>128 μg/mL),  C. krusei ATCC 2340 (MIC>128 μg/mL),  C. parapsilosis ATCC 22010(MIC>128 μg/mL).	No hemolytic activity information found.	Cyclic (Stapled)	Acetylation	Amidation	①The Ⓧ (position: 9 and 13) in sequence indicates S5 (2-amino-2-methylhept-6-enoic acid). ②Ⓧ (9) and Ⓧ (13) are cross-linked by hydrocarbon stapling respectively.	L	No cytotoxicity information found in the reference	34439006	Antibiotics (Basel). 2021 Aug 9;10(8):956. 	Zheng M, Wang R, Chen S, Zou Y, Yan L, Zhao L, Li X.	Design, Synthesis and Antifungal Activity of Stapled Aurein1.4 Peptides.	Stapled AMP					
DRAMP29360	LⓧRRLⓧR	LXRRLXR	LARRLAR	7	stRRL	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		α-helical conformation in an aqueous environment(phosphate buffered saline, PBS), negative electric environment(sodium dodecyl sulfate, SDS), and hydrophobic environment(tri-fluoroethyl alcohol, TFE).	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-negative bacteria.	[Ref.37531494]Gram-negativebacteria:E. coli ATCC25922(MIC=8.00 μM), E. coli UB1005(MIC=4.00 μM), E. coli K99(MIC=8.00 μM), E. coli 987P(MIC= 4.00 μM), S. typhimurium ATCC14028(MIC=32.00 μM), P. aeruginosa PAO1(MIC=16.00 μM).	[Ref.37531494]MHC>256 μM. MHC is the minimum peptide concentration that caused >5% hemolysis of hRBCs. 	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates S-(2,4)-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling respectively.	L	[Ref.37531494]<10% cytotoxicity against human embryonic kidney cells (HEK293T) and intestinal porcine enterocyte cells (IPEC-J2) up to 128 μM. 71.50% cell viability against murine macrophage cells (RAW264.7) at 128 μM.	37531494	J Med Chem. 2023 Aug 24;66(16):11414-11427. 	Shao C, Jian Q, Li B, Zhu Y, Yu W, Li Z, Shan A.	Ultrashort All-Hydrocarbon Stapled α-Helix Amphiphile as a Potent and Stable Antimicrobial Compound. 	Stapled AMP					
DRAMP29361	LⓧRLLⓧR	LXRLLXR	LARLLAR	7	stRLL	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-negative bacteria.	[Ref.37531494]Gram-negativebacteria:E. coli ATCC25922(MIC=16.00 μM), E. coli UB1005(MIC=8.00 μM), E. coli K99(MIC=16.00 μM), E. coli 987P(MIC= 8.00 μM), S. typhimurium ATCC14028(MIC=32.00 μM), P. aeruginosa PAO1(MIC=32.00 μM).	[Ref.37531494]MHC=128 μM. MHC is the minimum peptide concentration that caused >5% hemolysis of hRBCs. 8.54% and 29.1% hemolysis at  128 and 256 μM against  hRBCs.	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates S-(2,4)-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling respectively.	L	[Ref.37531494]<10% cytotoxicity against human embryonic kidney cells (HEK293T) and intestinal porcine enterocyte cells (IPEC-J2) up to 128 μM. 21.78% cell viability against murine macrophage cells (RAW264.7) at 128 μM.	37531494	J Med Chem. 2023 Aug 24;66(16):11414-11427. 	Shao C, Jian Q, Li B, Zhu Y, Yu W, Li Z, Shan A.	Ultrashort All-Hydrocarbon Stapled α-Helix Amphiphile as a Potent and Stable Antimicrobial Compound. 	Stapled AMP					
DRAMP29362	LⓧLRLⓧR	LXLRLXR	LALRLAR	7	stLRL	No entry found		Synthetic construct	Antimicrobial, Antibacterial, Anti-Gram-		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Function: Antibacterial activity against Gram-negative bacteria.	[Ref.37531494]Gram-negativebacteria:E. coli ATCC25922(MIC=16.00 μM), E. coli UB1005(MIC=16.00 μM), E. coli K99(MIC=16.00 μM), E. coli 987P(MIC=8.00 μM), S. typhimurium ATCC14028(MIC=64.00 μM), P. aeruginosa PAO1(MIC=32.00 μM).	[Ref.37531494]MHC>256 μM. MHC is the minimum peptide concentration that caused >5% hemolysis of hRBCs. 	Cyclic (Stapled)	Free	Amidation	①The Ⓧ (position: 2 and 6) in sequence indicates S-(2,4)-pentenyl alanine. ②Ⓧ (2) and Ⓧ (6) are cross-linked by hydrocarbon stapling respectively.	L	[Ref.37531494]<10% cytotoxicity against human embryonic kidney cells (HEK293T) and intestinal porcine enterocyte cells (IPEC-J2) up to 128 μM. 55.18% cell viability against murine macrophage cells (RAW264.7) at 128 μM.	37531494	J Med Chem. 2023 Aug 24;66(16):11414-11427. 	Shao C, Jian Q, Li B, Zhu Y, Yu W, Li Z, Shan A.	Ultrashort All-Hydrocarbon Stapled α-Helix Amphiphile as a Potent and Stable Antimicrobial Compound. 	Stapled AMP					
DRAMP32456	GIMⓍSLMⓍKLAAHIAK	GIMXSLMXKLAAHIAK		16	HYL-18	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 (IC50=1.48±0.15 µmol/L); A549 (IC50=2.38±0.38 µmol/L); Hep-G2 (IC50=2.67±0.29 µmol/L)	Not available	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Human Splenic Fibroblasts (HSF): IC50=12.78±0.33 µg/mL; Endometrial epithelial cells(HEEC): IC50=2.66±0.34 µg/mL; HPDE6c7 Human Pancreatic Duct Epithelial Cell: IC50=3.45±0.20 µg/mL; Normal Human Hepatic Cell (Lo-2): IC50=2.91±0.07 µg/mL; NCM460: IC50=3.04±0.10 µg/mL; CCD-19Lu: IC50=4.76±0.03 µg/mL	Not available	Not available	Not available	Structural modification and antitumor activity of antimicrobial peptide HYL	Stapled AMP					
DRAMP32458	GIMSⓍLMKⓍLAAHIAK	GIMSXLMKXLAAHIAK		16	HYL-17	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 (IC50=1.54±0.32 µmol/L); A549 (IC50=5.56±0.41 µmol/L); Hep-G2 (IC50=9.70±0.50 µmol/L)	Not available	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ(5) and Ⓧ (9) are cross-linked by hydrocarbon stapling	L	Human Splenic Fibroblasts (HSF): IC50=12.78±0.33 µg/mL; Endometrial epithelial cells(HEEC): IC50=2.66±0.34 µg/mL; HPDE6c7 Human Pancreatic Duct Epithelial Cell: IC50=3.45±0.20 µg/mL; Normal Human Hepatic Cell (Lo-2): IC50=2.91±0.07 µg/mL; NCM460: IC50=3.04±0.10 µg/mL; CCD-19Lu: IC50=4.76±0.03 µg/mL	Not available	Not available	Not available	Structural modification and antitumor activity of antimicrobial peptide HYL	Stapled AMP					
DRAMP32462	GIMSSLMⓍKLAⓍHIAK	GIMSSLMXKLAXHIAK		16	HYL-16	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 (IC50=1.90±0.35 µmol/L); A549 (IC50=2.36±0.23 µmol/L); Hep-G2 (IC50=2.45±0.22 µmol/L)	Not available	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ(8) and Ⓧ (12) are cross-linked by hydrocarbon stapling	L	Human Splenic Fibroblasts (HSF): IC50=12.78±0.33 µg/mL; Endometrial epithelial cells(HEEC): IC50=2.66±0.34 µg/mL; HPDE6c7 Human Pancreatic Duct Epithelial Cell: IC50=3.45±0.20 µg/mL; Normal Human Hepatic Cell (Lo-2): IC50=2.91±0.07 µg/mL; NCM460: IC50=3.04±0.10 µg/mL; CCD-19Lu: IC50=4.76±0.03 µg/mL	Not available	Not available	Not available	Structural modification and antitumor activity of antimicrobial peptide HYL	Stapled AMP					
DRAMP32464	GIMSSLMKⓍLAAⓍIAK	GIMSSLMKXLAAXIAK		16	HYL-15	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 (IC50=2.52±0.42 µmol/L); A549 (IC50=3.17±0.33 µmol/L); Hep-G2 (IC50=3.38±0.26 µmol/L)	Not available	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ(9) and Ⓧ (13) are cross-linked by hydrocarbon stapling	L	Human Splenic Fibroblasts (HSF): IC50=12.78±0.33 µg/mL; Endometrial epithelial cells(HEEC): IC50=2.66±0.34 µg/mL; HPDE6c7 Human Pancreatic Duct Epithelial Cell: IC50=3.45±0.20 µg/mL; Normal Human Hepatic Cell (Lo-2): IC50=2.91±0.07 µg/mL; NCM460: IC50=3.04±0.10 µg/mL; CCD-19Lu: IC50=4.76±0.03 µg/mL	Not available	Not available	Not available	Structural modification and antitumor activity of antimicrobial peptide HYL	Stapled AMP					
DRAMP32467	GIMSSLMKKLⓍAHIⓍK	GIMSSLMKKLXAHIXK		16	HYL-14	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: A549 (IC50=1.36±0.35 µmol/L); HCT 116 (IC50=1.61±0.27 µmol/L); Hep-G2 (IC50=1.67±0.25 µmol/L)	Not available	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ(11) and Ⓧ (15) are cross-linked by hydrocarbon stapling	L	Human Splenic Fibroblasts (HSF): IC50=12.78±0.33 µg/mL; Endometrial epithelial cells(HEEC): IC50=2.66±0.34 µg/mL; HPDE6c7 Human Pancreatic Duct Epithelial Cell: IC50=3.45±0.20 µg/mL; Normal Human Hepatic Cell (Lo-2): IC50=2.91±0.07 µg/mL; NCM460: IC50=3.04±0.10 µg/mL; CCD-19Lu: IC50=4.76±0.03 µg/mL	Not available	Not available	Not available	Structural modification and antitumor activity of antimicrobial peptide HYL	Stapled AMP					
DRAMP32481	TAWYANFⓍKLLⓍ	TAWYANFXKLLX		12	DPMI-δ-F	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Not available	Not available	Stapled: Ⓧ(8) and Ⓧ (12) are cross-linked by hydrocarbon stapling	L	Not available	Not available	Not available	Not available	Not available	Stapled AMP					
DRAMP32482	TAWYⓍNFEⓍLLR	TAWYXNFEXLLR		12	DPMI-δ-D	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Not available	Not available	Stapled: Ⓧ(5) and Ⓧ (9) are cross-linked by hydrocarbon stapling	L	Not available	Not available	Not available	Not available	Not available	Stapled AMP					
DRAMP32483	TAWYⓍNFEKLLⓍ	TAWYXNFEKLLX		12	DPMI-δ-B	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Not available	Not available	Stapled: Ⓧ(5) and Ⓧ (12) are cross-linked by hydrocarbon stapling	L	Not available	Not available	Not available	Not available	Not available	Stapled AMP					
DRAMP32484	WYANFⓍKLLⓍ	WYANFXKLLX		10	DPMI-δ-E	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Not available	Not available	Stapled: Ⓧ(6) and Ⓧ (10) are cross-linked by hydrocarbon stapling	L	Not available	Not available	Not available	Not available	Not available	Stapled AMP					
DRAMP32485	WYⓍNFEⓍLLR	WYXNFEXLLR		10	DPMI-δ-C	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Not available	Not available	Stapled: Ⓧ(3) and Ⓧ (7) are cross-linked by hydrocarbon stapling	L	Not available	Not available	Not available	Not available	Not available	Stapled AMP					
DRAMP32486	WYⓍNFEKLLⓍ	WYXNFEKLLX		10	DPMI-δ-A	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Not available	Not available	Stapled: Ⓧ(3) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	Not available	Not available	Not available	Not available	Stapled AMP					
DRAMP33751	IKLSKETKKNLKKVLKGAIKGⓍIAVⓍKMV	IKLSKETKKNLKKVLKGAIKGXIAVXKMV	IKLSKETKKNLKKVLKGAIKGSIAVSKMV	29	H-19, Hymenochirin-1B [P5K,D9K; A18,A22 S5]	W8PRC4		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: A549 (IC50=1.00±0.36 μM); HepG2 (IC50=1.64±0.36 μM); HCT 116 (IC50=1.78±0.35  μM)	Not available	Cyclic (Stapled)	Free	Free	Stapled: Ⓧ(18) and Ⓧ (22) are cross-linked by hydrocarbon stapling	L	Not available	30789695	ACS Chem Biol. 2019 Mar 15;14(3):516-525. 	Li Y, Zhang Y, Wu M, Chang Q, Hu H, Zhao X.	Improving Selectivity, Proteolytic Stability, and Antitumor Activity of Hymenochirin-1B: A Novel Glycosylated Staple Strategy	Stapled AMP					
DRAMP34558	GLFAVⓍKKVASVⓍKGL	GLFAVXKKVASVXKGL	GLFAVCKKVASVCKG	16	AC-CCSP-5-Dp	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=12.1 μM); U87 (IC50=14.72 μM); A549 (IC50=16.37 μM); C4-2B (IC50=35.84 μM)	Not available	Cyclic (Stapled)	Acetylization	Amidation	Stapled: Ⓧ(6) and Ⓧ (13) are cross-linked by hydrocarbon stapling	L	Not available	37804993	Bioorg Med Chem Lett. 2023 Nov 15;96:129499.	Shen H, Zhang N, Kong X, Wang N, Hu HG, Cong W, Liu C. 	Benzyl stapled modification and anticancer activity of antimicrobial peptide A4K14-Citropin 1.1	Stapled AMP					
DRAMP34559	GLFAVⓍKKVASVⓍKGL	GLFAVXKKVASVXKGL	GLFAVCKKVASVCKG	16	AC-CCSP-5-Dp	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 (IC50=7.44µM); MCF-7 (IC50=7.47µM); U-87MG ATCC (IC50=9.2µM)	Not available	Cyclic (Stapled)	Acetylization	Amidation	Stapled: Ⓧ(6) and Ⓧ (13) are cross-linked by hydrocarbon stapling	L	Not available	37804993	Bioorg Med Chem Lett. 2023 Nov 15;96:129499.	Shen H, Zhang N, Kong X, Wang N, Hu HG, Cong W, Liu C. 	Benzyl stapled modification and anticancer activity of antimicrobial peptide A4K14-Citropin 1.1	Stapled AMP					
DRAMP34929	LSQETFSDⓍWKLLPEⓍ	LSQETFSDXWKLLPEX	LSQETFSDWKLLPE	16	SAH-p53-1	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Free	Free	Stapled: Ⓧ(9) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	22148351	J Am Chem Soc. 2012 Jan 11;134(1):103-7. 	Baek S, Kutchukian PS, Verdine GL, Huber R, Holak TA, Lee KW, Popowicz GM.	Structure of the stapled p53 peptide bound to Mdm2	Stapled AMP					
DRAMP34930	LSQEⓍFSDLWKⓍLPEN	LSQEXFSDLWKXLPEN	LSQEFSDLWKLPEN	16	SAH-p53-2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Free	Free	Stapled: Ⓧ(5) and Ⓧ (12) are cross-linked by hydrocarbon stapling	L	Not available	22148351	J Am Chem Soc. 2012 Jan 11;134(1):103-8. 	Baek S, Kutchukian PS, Verdine GL, Huber R, Holak TA, Lee KW, Popowicz GM.	Structure of the stapled p53 peptide bound to Mdm2	Stapled AMP					
DRAMP34931	LSQⓍTFSDLWKLLⓍEN	LSQXTFSDLWKLLXEN	LSQTFSDLWKLLEN	16	SAH-p53-3	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Free	Free	Stapled: Ⓧ(4) and Ⓧ (14) are cross-linked by hydrocarbon stapling	L	Not available	22148351	J Am Chem Soc. 2012 Jan 11;134(1):103-9. 	Baek S, Kutchukian PS, Verdine GL, Huber R, Holak TA, Lee KW, Popowicz GM.	Structure of the stapled p53 peptide bound to Mdm2	Stapled AMP					
DRAMP34932	LSQETFⓍDLWKLLⓍEN	LSQETFXDLWKLLXEN	LSQETFDLWKLLEN	16	SAH-p53-4	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Free	Free	Stapled: Ⓧ(7) and Ⓧ (14) are cross-linked by hydrocarbon stapling	L	Not available	22148351	J Am Chem Soc. 2012 Jan 11;134(1):103-10. 	Baek S, Kutchukian PS, Verdine GL, Huber R, Holak TA, Lee KW, Popowicz GM.	Structure of the stapled p53 peptide bound to Mdm2	Stapled AMP					
DRAMP34933	LSQETFⓍNLWKLLⓍQN	LSQETFXNLWKLLXQN	LSQETFNLWKLLQN	16	SAH-p53-5	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Free	Free	Stapled: Ⓧ(7) and Ⓧ (14) are cross-linked by hydrocarbon stapling	L	Not available	22148351	J Am Chem Soc. 2012 Jan 11;134(1):103-11. 	Baek S, Kutchukian PS, Verdine GL, Huber R, Holak TA, Lee KW, Popowicz GM.	Structure of the stapled p53 peptide bound to Mdm2	Stapled AMP					
DRAMP34934	LSQQTFⓍNLWRLLⓍQN	LSQQTFXNLWRLLXQN	LSQQTFNLWRLLQN	16	SAH-p53-6	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Free	Free	Stapled: Ⓧ(7) and Ⓧ (14) are cross-linked by hydrocarbon stapling	L	Not available	22148351	J Am Chem Soc. 2012 Jan 11;134(1):103-12. 	Baek S, Kutchukian PS, Verdine GL, Huber R, Holak TA, Lee KW, Popowicz GM.	Structure of the stapled p53 peptide bound to Mdm2	Stapled AMP					
DRAMP34935	QSQQTFⓍNLWKLLⓍQN	QSQQTFXNLWKLLXQN	QSQQTFNLWKLLQN	16	SAH-p53-7	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Free	Free	Stapled: Ⓧ(7) and Ⓧ (14) are cross-linked by hydrocarbon stapling	L	Not available	22148351	J Am Chem Soc. 2012 Jan 11;134(1):103-13. 	Baek S, Kutchukian PS, Verdine GL, Huber R, Holak TA, Lee KW, Popowicz GM.	Structure of the stapled p53 peptide bound to Mdm2	Stapled AMP					
DRAMP34936	QSQQTFⓍNLWRLLⓍQN	QSQQTFXNLWRLLXQN	QSQQTFNLWRLLQN	16	SAH-p53-8	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Free	Free	Stapled: Ⓧ(7) and Ⓧ (14) are cross-linked by hydrocarbon stapling	L	Not available	22148351	J Am Chem Soc. 2012 Jan 11;134(1):103-14. 	Baek S, Kutchukian PS, Verdine GL, Huber R, Holak TA, Lee KW, Popowicz GM.	Structure of the stapled p53 peptide bound to Mdm2	Stapled AMP					
DRAMP34937	QSQQTFⓍNLWRLLⓍQN	QSQQTFXNLWRLLXQN	QSQQTFNLWRLLQN	16	SAH-p53-8	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Free	Free	Stapled: Ⓧ(7) and Ⓧ (14) are cross-linked by hydrocarbon stapling	L	Not available	22148351	J Am Chem Soc. 2012 Jan 11;134(1):103-14. 	Baek S, Kutchukian PS, Verdine GL, Huber R, Holak TA, Lee KW, Popowicz GM.	Structure of the stapled p53 peptide bound to Mdm2	Stapled AMP					
DRAMP35008	BENPEⓍILDEHVⓍRVM	BENPEXILDEHVXRVM	BENPERILDEHVSRVM	16	fStAx-1	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	FITC	Amidation	Stapled: Ⓧ(6) and Ⓧ (13) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-8.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35009	BENPESILDⓍHVQⓍVM	BENPESILDXHVQXVM	BENPESILDSHVQSVM	16	fStAx-2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	FITC	Amidation	Stapled: Ⓧ(10) and Ⓧ (14) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-9.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35010	BENPEⓍILDⓍHVQRVM	BENPEXILDXHVQRVM	BENPESILDSHVQRVM	16	fStAx-3	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	FITC	Amidation	Stapled: Ⓧ(6) and Ⓧ (10) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-10.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35011	BPEⓍILDⓍHVQRVM	BPEXILDXHVQRVM	BPESILDSHVQRVM	14	fStAx-31	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	FITC	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-11.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35012	XPQⓍILDⓍHVRRVMR	XPQXILDXHVRRVMR	XPQSILDSHVRRVMR	15	fStAx-32	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	FITC	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-12.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35013	XPQⓍILDⓍHVRRVWR	XPQXILDXHVRRVWR	XPQSILDSHVRRVWR	15	fStAx-33	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	FITC	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-13.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35014	XRWPQⓍILDⓍHVRRVWR	XRWPQXILDXHVRRVWR	XRWPQSILDSHVRRVWR	17	fStAx-34	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	FITC	Amidation	Stapled: Ⓧ(6) and Ⓧ (10) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-14.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35015	XRRWPQⓍILDⓍHVRRVWR	XRRWPQXILDXHVRRVWR	XRRWPQSILDSHVRRVWR	18	fStAx-35	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	FITC	Amidation	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-15.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35016	XRRWPRⓍILDⓍHVRRVWR	XRRWPRXILDXHVRRVWR	XRRWPRSILDSHVRRVWR	18	fStAx-35R	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	FITC	Amidation	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-16.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35017	XRRWPQⓍILHⓍDVRRVWR	XRRWPQXILHXDVRRVWR	XRRWPQSILHSDVRRVWR	18	fStAx-40	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	FITC	Amidation	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-17.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35018	XRRWPQⓍILHⓍDVRRVAR	XRRWPQXILHXDVRRVAR	XRRWPQSILHSDVRRVAR	18	fStAx-41	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	FITC	Amidation	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-18.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35019	XRRWPRⓍILHⓍDVRRVAR	XRRWPRXILHXDVRRVAR	XRRWPRSILHSDVRRVAR	18	fStAx-41R	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	FITC		Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-19.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35020	RRWPQⓍILDⓍHVRRVWR	RRWPQXILDXHVRRVWR	RRWPQSILDSHVRRVWR	17	aStAx-35	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(6) and Ⓧ (10) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-20.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35021	RRWPRⓍILHⓍDVRRVAR	RRWPRXILHXDVRRVAR	XRRWPRSILHSDVRRVAR	17	aStAx-41R	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(6) and Ⓧ (10) are cross-linked by hydrocarbon stapling	L	Not available	23071338	 Proc Natl Acad Sci U S A. 2012 Oct 30;109(44):17942-21.	Grossmann TN, Yeh JT, Bowman BR, Chu Q, Moellering RE, Verdine GL.	Inhibition of oncogenic Wnt signaling through direct targeting of β-catenin	Stapled AMP					
DRAMP35127	QSQQTFⓍNLWRLLⓍQN	QSQQTFXNLWRLLXQN	QSQQTFRNLWRLLSQN	16	ATSP-1800	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(7) and Ⓧ (14) are cross-linked by hydrocarbon stapling	L	Not available	23946421	Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):E3445-54.	Chang YS, Graves B, Guerlavais V, Tovar C, Packman K, To KH, Olson KA, Kesavan K, Gangurde P, Mukherjee A, Baker T, Darlak K, Elkin C, Filipovic Z, Qureshi FZ, Cai H, Berry P, Feyfant E, Shi XE, Horstick J, Annis DA, Manning AM, Fotouhi N, Nash H, Vassilev LT, Sawyer TK.	Stapled α-helical peptide drug development: a potent dual inhibitor of MDM2 and MDMX for p53-dependent cancer therapy	Stapled AMP					
DRAMP35128	QSQQTFⓍNLWRLLⓍQN	QSQQTFXNLWRLLXQN	QSQQTFRNLWRLLSQN	16	ATSP-1800	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(7) and Ⓧ (14) are cross-linked by hydrocarbon stapling	L	Not available	23946421	Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):E3445-54.	Chang YS, Graves B, Guerlavais V, Tovar C, Packman K, To KH, Olson KA, Kesavan K, Gangurde P, Mukherjee A, Baker T, Darlak K, Elkin C, Filipovic Z, Qureshi FZ, Cai H, Berry P, Feyfant E, Shi XE, Horstick J, Annis DA, Manning AM, Fotouhi N, Nash H, Vassilev LT, Sawyer TK.	Stapled α-helical peptide drug development: a potent dual inhibitor of MDM2 and MDMX for p53-dependent cancer therapy	Stapled AMP					
DRAMP35131	LTFⓍHYWAQLⓍS	LTFXHYWAQLXS	LTFRHYWAQLSS	12	ATSP-3900	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	23946421	Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):E3445-56.	Chang YS, Graves B, Guerlavais V, Tovar C, Packman K, To KH, Olson KA, Kesavan K, Gangurde P, Mukherjee A, Baker T, Darlak K, Elkin C, Filipovic Z, Qureshi FZ, Cai H, Berry P, Feyfant E, Shi XE, Horstick J, Annis DA, Manning AM, Fotouhi N, Nash H, Vassilev LT, Sawyer TK.	Stapled α-helical peptide drug development: a potent dual inhibitor of MDM2 and MDMX for p53-dependent cancer therapy	Stapled AMP					
DRAMP35132	LTFⓍAYWAQLⓍS	LTFXAYWAQLXS	LTFRAYWAQLSS	12	ATSP-4641	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	23946421	Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):E3445-57.	Chang YS, Graves B, Guerlavais V, Tovar C, Packman K, To KH, Olson KA, Kesavan K, Gangurde P, Mukherjee A, Baker T, Darlak K, Elkin C, Filipovic Z, Qureshi FZ, Cai H, Berry P, Feyfant E, Shi XE, Horstick J, Annis DA, Manning AM, Fotouhi N, Nash H, Vassilev LT, Sawyer TK.	Stapled α-helical peptide drug development: a potent dual inhibitor of MDM2 and MDMX for p53-dependent cancer therapy	Stapled AMP					
DRAMP35133	LTFⓍEYWAQLⓍS	LTFXEYWAQLXS	LTFREYWAQLSS	12	ATSP-6935	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	23946421	Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):E3445-58.	Chang YS, Graves B, Guerlavais V, Tovar C, Packman K, To KH, Olson KA, Kesavan K, Gangurde P, Mukherjee A, Baker T, Darlak K, Elkin C, Filipovic Z, Qureshi FZ, Cai H, Berry P, Feyfant E, Shi XE, Horstick J, Annis DA, Manning AM, Fotouhi N, Nash H, Vassilev LT, Sawyer TK.	Stapled α-helical peptide drug development: a potent dual inhibitor of MDM2 and MDMX for p53-dependent cancer therapy	Stapled AMP					
DRAMP35134	LTFⓍEYWAQXⓍSAA	LTFXEYWAQXXSAA	LTFREYWAQXSSAA	14	ATSP-7041	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	23946421	Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):E3445-59.	Chang YS, Graves B, Guerlavais V, Tovar C, Packman K, To KH, Olson KA, Kesavan K, Gangurde P, Mukherjee A, Baker T, Darlak K, Elkin C, Filipovic Z, Qureshi FZ, Cai H, Berry P, Feyfant E, Shi XE, Horstick J, Annis DA, Manning AM, Fotouhi N, Nash H, Vassilev LT, Sawyer TK.	Stapled α-helical peptide drug development: a potent dual inhibitor of MDM2 and MDMX for p53-dependent cancer therapy	Stapled AMP					
DRAMP35135	LTAⓍEYWAQXⓍSAA	LTAXEYWAQXXSAA	LTAREYWAQXSSAA	14	ATSP-7342	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	23946421	Proc Natl Acad Sci U S A. 2013 Sep 3;110(36):E3445-60.	Chang YS, Graves B, Guerlavais V, Tovar C, Packman K, To KH, Olson KA, Kesavan K, Gangurde P, Mukherjee A, Baker T, Darlak K, Elkin C, Filipovic Z, Qureshi FZ, Cai H, Berry P, Feyfant E, Shi XE, Horstick J, Annis DA, Manning AM, Fotouhi N, Nash H, Vassilev LT, Sawyer TK.	Stapled α-helical peptide drug development: a potent dual inhibitor of MDM2 and MDMX for p53-dependent cancer therapy	Stapled AMP					
DRAMP35158	ISFⓍELLDYYⓍESGS	ISFXELLDYYXESGS	ISFRELLDYYSESGS	15	NYAD-36	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	24237936	Retrovirology. 2013 Nov 15;10:136.	Zhang H, Curreli F, Waheed AA, Mercredi PY, Mehta M, Bhargava P, Scacalossi D, Tong X, Lee S, Cooper A, Summers MF, Freed EO, Debnath AK. 	Not available	Stapled AMP					
DRAMP35159	ISFⓍELLDYYⓍED	ISFXELLDYYXED	ISFRELLDYYSED	13	NYAD-66	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	24237936	Retrovirology. 2013 Nov 15;10:137.	Zhang H, Curreli F, Waheed AA, Mercredi PY, Mehta M, Bhargava P, Scacalossi D, Tong X, Lee S, Cooper A, Summers MF, Freed EO, Debnath AK. 	Not available	Stapled AMP					
DRAMP35160	ISFⓍEWLQAYⓍEDE	ISFXEWLQAYXEDE	ISFREWLQAYSEDE	14	NYAD-67	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	24237936	Retrovirology. 2013 Nov 15;10:138.	Zhang H, Curreli F, Waheed AA, Mercredi PY, Mehta M, Bhargava P, Scacalossi D, Tong X, Lee S, Cooper A, Summers MF, Freed EO, Debnath AK. 	Not available	Stapled AMP					
DRAMP35549	DDEⓍEQFⓍYHLLXFNAV	DDEXEQFXYHLLXFNAV	DDESEQFSYHLLXFNAV	17	StRIP4	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-85. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35550	DDEⓍEQFⓍYHLXSFNAV	DDEXEQFXYHLXSFNAV	DDESEQFSYHLXSFNAV	17	StRIP5	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-86. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35551	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP6	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-87. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35552	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP6	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-87. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35553	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP6	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-87. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35554	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP6	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-87. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35555	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP6	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-87. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35556	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP7	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-88. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35557	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP7	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-88. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35558	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP7	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-88. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35559	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP7	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-88. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35560	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP7	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-88. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35561	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP8	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-89. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35562	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP8	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-89. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35563	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP8	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-89. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35564	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP8	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-89. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35565	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP8	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-89. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35566	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP9	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-90. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35567	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP9	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-90. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35568	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP9	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-90. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35569	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP9	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-90. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35570	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP9	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-90. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35571	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP10	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-91. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35572	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP10	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-91. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35573	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP10	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-91. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35574	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP10	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-91. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35575	DDEⓍEQFⓍYHLXSFNXV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLXSFNXV	17	StRIP10	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-91. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35576	DDEⓍEQFLYHⓍLSFNAV	DDEXEQFLYHXLSFNAV	DDEREQFLYHSLSFNAV	17	StRIP11	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-92. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35577	DDEⓍEQFⓍYHLⓍSFNⓍV	DDEXEQFXYHLXSFNXV	DDESEQFSYHLSSFNSV	17	StRIP12	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-93. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35578	DDEⓍEWFⓍYHLⓍSFNⓍV	DDEXEWFXYHLXSFNXV	DDESEWFSYHLSSFNSV	17	StRIP13	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-94. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35579	DDEⓍEWFⓍYHLⓍFFNⓍV	DDEXEWFXYHLXFFNXV	DDESEWFSYHLSFFNSV	17	StRIP14	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-95. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35580	DDEⓍQWFⓍYHLⓍFFNⓍV	DDEXQWFXYHLXFFNXV	DDESQWFSYHLSFFNSV	17	StRIP15	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-96. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35581	DNEⓍQWFⓍYHLⓍFFNⓍV	DNEXQWFXYHLXFFNXV	DNESQWFSYHLSFFNSV	17	StRIP16	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-97. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35582	NDEⓍQWFⓍYHLⓍFFNⓍV	NDEXQWFXYHLXFFNXV	NDESQWFSYHLSFFNSV	17	StRIP17	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-98. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35583	DDEⓍEFFⓍYHLLSFNAV	DDEXEFFXYHLLSFNAV	DDESEFFSYHLLSFNAV	17	StRIP3[Q905F]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-99. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35584	DDEⓍELFⓍYHLLSFNAV	DDEXELFXYHLLSFNAV	DDESELFSYHLLSFNAV	17	StRIP3[Q905L]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-100. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35585	DDEⓍEWFⓍYHLLSFNAV	DDEXEWFXYHLLSFNAV	DDESEWFSYHLLSFNAV	17	StRIP3[Q905W]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-101. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35586	DDEⓍEQFⓍYHLFFFNAV	DDEXEQFXYHLFFFNAV	DDESEQFSYHLFFFNAV	17	StRIP3[L911F,S912F]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-102. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35587	DDEⓍEQFⓍYHLFWFNAV	DDEXEQFXYHLFWFNAV	DDESEQFSYHLFWFNAV	17	StRIP3[L911F,S912W]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-103. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35588	DDEⓍEQFⓍYHLFYFNAV	DDEXEQFXYHLFYFNAV	DDESEQFSYHLFYFNAV	17	StRIP3[L911F,S912Y]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-104. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35589	DDQⓍEWFⓍYHLⓍFFNⓍV	DDQXEWFXYHLXFFNXV	DDQSEWFSYHLSFFNSV	17	StRIP14[E902Q]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-105. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35590	DNEⓍEWFⓍYHLⓍFFNⓍV	DNEXEWFXYHLXFFNXV	DNESEWFSYHLSFFNSV	17	StRIP14[D901N]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-106. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35591	NDEⓍEWFⓍYHLⓍFFNⓍV	NDEXEWFXYHLXFFNXV	NDESEWFSYHLSFFNSV	17	StRIP14[D900N]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-107. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35592	DNQⓍEWFⓍYHLⓍFFNⓍV	DNQXEWFXYHLXFFNXV	DNQSEWFSYHLSFFNSV	17	StRIP14[D901N,E902Q]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-108. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35593	NDQⓍEWFⓍYHLⓍFFNⓍV	NDQXEWFXYHLXFFNXV	NDQSEWFSYHLSFFNSV	17	StRIP14[D900N,E902Q]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-109. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35594	NNEⓍEWFⓍYHLⓍFFNⓍV	NNEXEWFXYHLXFFNXV	NNESEWFSYHLSFFNSV	17	StRIP14[D900N,D901N]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-110. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35595	NNQⓍEWFⓍYHLⓍFFNⓍV	NNQXEWFXYHLXFFNXV	NNQSEWFSYHLSFFNSV	17	StRIP14[D900N,D901N,E902Q]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-111. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35596	DDQⓍQWFⓍYHLⓍFFNⓍV	DDQXQWFXYHLXFFNXV	DDQSQWFSYHLSFFNSV	17	StRIP14[E902Q,E904Q]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-112. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35597	DNQⓍQWFⓍYHLⓍFFNⓍV	DNQXQWFXYHLXFFNXV	DNQSQWFSYHLSFFNSV	17	StRIP14[D901N,E902Q,E904Q]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-113. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35598	NDQⓍQWFⓍYHLⓍFFNⓍV	NDQXQWFXYHLXFFNXV	NDQSQWFSYHLSFFNSV	17	StRIP14[D900N,E902Q,E904Q]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-114. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35599	NNEⓍQWFⓍYHLⓍFFNⓍV	NNEXQWFXYHLXFFNXV	NNESQWFSYHLSFFNSV	17	StRIP14[D900N,D901N,E904Q]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-115. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35600	NNQⓍQWFⓍYHLⓍFFNⓍV	NNQXQWFXYHLXFFNXV	NNQSQWFSYHLSFFNSV	17	StRIP14[D900N,D901N,E902Q,E904Q]	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Binds Rab8a(GppNHp) and is able to inhibit a Rab8a PPI.		Not available	Cyclic (Stapled)	Fluorescein-O2OC-	Free	Stapled: Ⓧ(4) and Ⓧ (8), Ⓧ(12) and Ⓧ (16) are cross-linked by hydrocarbon stapling	L	Not available	27336832	ACS Chem Biol. 2016 Aug 19;11(8):2375-116. 	Cromm PM, Spiegel J, Küchler P, Dietrich L, Kriegesmann J, Wendt M, Goody RS, Waldmann H, Grossmann TN.	Protease-Resistant and Cell-Permeable Double-Stapled Peptides Targeting the Rab8a GTPase	Stapled AMP					
DRAMP35711	ALPISTVRⓍVALⓍRRL	ALPISTVRXVALXRRL	ALPISTVRSVALSRRL	16	P2shortA	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: A549 (50% Killing=10 μM); PC-9 (80% Killing=10 μM); NCI-H358 (98% Killing=10 μM)	Not available	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ(9) and Ⓧ (13) are cross-linked by hydrocarbon stapling	L	Not available	32104498	Theranostics. 2020 Jan 12;10(5):2008-2028. 	Bouclier C, Simon M, Laconde G, Pellerano M, Diot S, Lantuejoul S, Busser B, Vanwonterghem L, Vollaire J, Josserand V, Legrand B, Coll JL, Amblard M, Hurbin A, Morris MC. 	Stapled peptide targeting the CDK4/Cyclin D interface combined with Abemaciclib inhibits KRAS mutant lung cancer growth	Stapled AMP					
DRAMP35725	PQⓍILDQHVⓍRVMK	PQRXILDQHVXRVMK	PQRILDQHVSRVMK	15	SAHPA1	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(3) and Ⓧ (10) are cross-linked by hydrocarbon stapling	L	Not available	32550000	Cell Discov. 2020 Jun 9;6:35.	Liao H, Li X, Zhao L, Wang Y, Wang X, Wu Y, Zhou X, Fu W, Liu L, Hu HG, Chen YG. 	A PROTAC peptide induces durable β-catenin degradation and suppresses Wnt-dependent intestinal cancer	Stapled AMP					
DRAMP35726	PQⓍILDQHVⓍRVMKXALAPYIP	PQRXILDQHVXRVMKXALAPYIP	PQRILDQHVSRVMKXALAPYIP	23	SAHPA1-VHLL	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(3) and Ⓧ (10) are cross-linked by hydrocarbon stapling	L	Not available	32550000	Cell Discov. 2020 Jun 9;6:36.	Liao H, Li X, Zhao L, Wang Y, Wang X, Wu Y, Zhou X, Fu W, Liu L, Hu HG, Chen YG. 	A PROTAC peptide induces durable β-catenin degradation and suppresses Wnt-dependent intestinal cancer	Stapled AMP					
DRAMP35727	RRWPRⓍILDⓍHVRRVWR	RRWPRXILDXHVRRVWR	XRRWPRSILDSHVRRVWR	17	xStAx, aStAx-35R	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(6) and Ⓧ (10) are cross-linked by hydrocarbon stapling	L	Not available	32550000	Cell Discov. 2020 Jun 9;6:37.	Liao H, Li X, Zhao L, Wang Y, Wang X, Wu Y, Zhou X, Fu W, Liu L, Hu HG, Chen YG. 	A PROTAC peptide induces durable β-catenin degradation and suppresses Wnt-dependent intestinal cancer	Stapled AMP					
DRAMP35728	RRWPRⓍlLDⓍHVRRVWRXALAPYIP	RRWPRXlLDXHVRRVWRXALAPYIP	RRWPRSlLDSHVRRVWRXALAPYIP	25	xStAx-VHLL	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Inhibit wnt/β-catenin signaling pathway		Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(6) and Ⓧ (10) are cross-linked by hydrocarbon stapling	Mix	Not available	32550000	Cell Discov. 2020 Jun 9;6:38.	Liao H, Li X, Zhao L, Wang Y, Wang X, Wu Y, Zhou X, Fu W, Liu L, Hu HG, Chen YG. 	A PROTAC peptide induces durable β-catenin degradation and suppresses Wnt-dependent intestinal cancer	Stapled AMP					
DRAMP35749	GⓍFAVIKKⓍASVIKGL	GXFAVIKKXASVIKGL	GRFAVIKKSASVIKGL	16	A4K14-citropin1.1-Sp6	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=23.78 μM); A549 (IC50=30.19 μM); U87 (IC50=34.49 μM); C4-2B (IC50=35.84 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(2) and Ⓧ (9) are cross-linked by hydrocarbon stapling	L	Not available	33363118	 Front Chem. 2020 Dec 10;8:616147.	Wang N, Xie G, Liu C, Cong W, He S, Li Y, Fan L, Hu HG. 	Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides	Stapled AMP					
DRAMP35750	GLFAVⓍKKVASVⓍKGL	GLFAVXKKVASVXKGL	GLFAVRKKVASVSKGL	16	A4K14-citropin1.1-Sp7	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=12.1 μM); U87 (IC50=14.72 μM); A549 (IC50=16.37 μM); C4-2B (IC50=35.84 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(6) and Ⓧ (13) are cross-linked by hydrocarbon stapling	L	Not available	33363118	 Front Chem. 2020 Dec 10;8:616148.	Wang N, Xie G, Liu C, Cong W, He S, Li Y, Fan L, Hu HG. 	Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides	Stapled AMP					
DRAMP35751	GLFAVⓍKKVASVⓍKGL	GLFAVXKKVASVXKGL	GLFAVRKKVASVSKGL	16	A4K14-citropin1.1-Sp7	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 (IC50=7.44µM); MCF-7 (IC50=7.47µM); U-87MG ATCC (IC50=9.2µM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(6) and Ⓧ (13) are cross-linked by hydrocarbon stapling	L	Not available	33363118	 Front Chem. 2020 Dec 10;8:616148.	Wang N, Xie G, Liu C, Cong W, He S, Li Y, Fan L, Hu HG. 	Design, Synthesis, and Antitumor Activities Study of Stapled A4K14-Citropin 1.1 Peptides	Stapled AMP					
DRAMP35824	ILGKLLⓍTAAⓍLLSNL	ILGKLLXTAAXLLSNL	/	16	Alyteserin-2a-Sp1	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MDA-MB-231 (IC50=1.6μM); A549 (IC50=3.6±1.0 μM); MCF-7  (IC50=4.9±0.91 μM); U87 (IC50=9.8±0.11 μM)	Not available	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ are cross-linked by hydrocarbon stapling	L	Not available	34989078	J Pept Sci. 2022 Jul;28(7):e3401	Yu Z, Tang H, Cong W, Gao F, Li H, Hu H, Wang X, He S.	Hydrocarbon stapling modification of peptide alyteserin-2a: Discovery of novel stapled peptide antitumor agents	Stapled AMP					
DRAMP35825	ILⓍKLLⓍTAAGLLSNL	ILXKLLXTAAGLLSNL	/	16	Alyteserin-2a-Sp2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: A549 (IC50=10.9±2.7 μM); MDA-MB-231 (IC50=3.6μM); U87 (IC50=5.2±0.68 μM); MCF-7  (IC50=5.8±0.80 μM)	Not available	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ are cross-linked by hydrocarbon stapling	L	Not available	34989078	J Pept Sci. 2022 Jul;28(7):e3402	Yu Z, Tang H, Cong W, Gao F, Li H, Hu H, Wang X, He S.	Hydrocarbon stapling modification of peptide alyteserin-2a: Discovery of novel stapled peptide antitumor agents	Stapled AMP					
DRAMP35826	ILGKLLSTAⓍGLLⓍNL	ILGKLLSTAXGLLXNL	/	16	Alyteserin-2a-Sp3	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MDA-MB-231 (IC50=0.86μM); MCF-7  (IC50=0.99±0.21 μM); A549 (IC50=2.8±0.90 μM); U87 (IC50=3.9±0.010 μM)	Not available	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ are cross-linked by hydrocarbon stapling	L	Not available	34989078	J Pept Sci. 2022 Jul;28(7):e3403	Yu Z, Tang H, Cong W, Gao F, Li H, Hu H, Wang X, He S.	Hydrocarbon stapling modification of peptide alyteserin-2a: Discovery of novel stapled peptide antitumor agents	Stapled AMP					
DRAMP35827	ILGKLLⓍTAAGLLⓍNL	ILGKLLXTAAGLLXNL	/	16	Alyteserin-2a-Sp4	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: U87 (IC50=10.1±1.6 μM); A549 (IC50=11.6±2.2 μM); MCF-7  (IC50=8.5±1.4 μM); MDA-MB-231 (IC50=9.2μM)	Not available	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ(7) and Ⓧ (14) are cross-linked by hydrocarbon stapling	L	Not available	34989078	J Pept Sci. 2022 Jul;28(7):e3404	Yu Z, Tang H, Cong W, Gao F, Li H, Hu H, Wang X, He S.	Hydrocarbon stapling modification of peptide alyteserin-2a: Discovery of novel stapled peptide antitumor agents	Stapled AMP					
DRAMP35828	ILⓍKLLSTAⓍGLLSNL	ILXKLLSTAXGLLSNL	/	16	Alyteserin-2a-Sp5	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=5.2±0.91 μM); A549 (IC50=8.4±1.8 μM); U87 (IC50=9.5±2.0 μM)	Not available	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ(3) and Ⓧ (10) are cross-linked by hydrocarbon stapling	L	Not available	34989078	J Pept Sci. 2022 Jul;28(7):e3405	Yu Z, Tang H, Cong W, Gao F, Li H, Hu H, Wang X, He S.	Hydrocarbon stapling modification of peptide alyteserin-2a: Discovery of novel stapled peptide antitumor agents	Stapled AMP					
DRAMP35849	VⓍRFKⓍFFRKLKKLV	VXRFKXFFRKLKKLV	VSRFKSFFRKLKKLV	15	B1-L-1	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: U87 (IC50=12.24±1.23 μM); Huh-7 (IC50=13.87±1.25 μM); MCF-7  (IC50=15.89±0.92 μM)	HC50>80 μM	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ are cross-linked by hydrocarbon stapling	L	Not available	35464194	 Erratum in: Front Chem. 2023 Oct 27;11:1285116. 	Su Z, Liu C, Cong W, He S, Su L, Hu H.	Design, Synthesis, and Antitumor Activity Study of All-Hydrocarbon-Stapled B1-Leu Peptides	Stapled AMP					
DRAMP35850	VKRFⓍKFFⓍKLKKLV	VKRFXKFFXKLKKLV	VKRFSKFFSKLKKLV	15	B1-L-2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: Huh-7 (IC50=6.18±0.63 μM); MCF-7  (IC50=6.591±0.42 μM); U87 (IC50=8.86±1.43 μM)	HC50>80 μM	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ are cross-linked by hydrocarbon stapling	L	Not available	35464194	 Erratum in: Front Chem. 2023 Oct 27;11:1285117. 	Su Z, Liu C, Cong W, He S, Su L, Hu H.	Design, Synthesis, and Antitumor Activity Study of All-Hydrocarbon-Stapled B1-Leu Peptides	Stapled AMP					
DRAMP35851	VKRFKⓍFFRⓍLKKLV	VKRFKXFFRXLKKLV	VKRFKSFFRSLKKLV	15	B1-L-3	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: U87 (IC50=3.59±1.20 μM); MCF-7  (IC50=5.478±0.89 μM); Huh-7 (IC50=5.96±0.54 μM)	HC50>80 μM	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ are cross-linked by hydrocarbon stapling	L	Not available	35464194	 Erratum in: Front Chem. 2023 Oct 27;11:1285118. 	Su Z, Liu C, Cong W, He S, Su L, Hu H.	Design, Synthesis, and Antitumor Activity Study of All-Hydrocarbon-Stapled B1-Leu Peptides	Stapled AMP					
DRAMP35852	VKRFKKFFⓍKLKⓍLV	VKRFKKFFXKLKXLV	VKRFKKFFSKLKSLV	15	B1-L-4	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: Huh-7 (IC50=13.41±1.92 μM); MCF-7  (IC50=15.20±1.02 μM); U87 (IC50=17.91±3.23 μM)	HC50>80 μM	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ are cross-linked by hydrocarbon stapling	L	Not available	35464194	 Erratum in: Front Chem. 2023 Oct 27;11:1285119. 	Su Z, Liu C, Cong W, He S, Su L, Hu H.	Design, Synthesis, and Antitumor Activity Study of All-Hydrocarbon-Stapled B1-Leu Peptides	Stapled AMP					
DRAMP35853	VⓍRFKKFFⓍKLKKLV	VXRFKKFFXKLKKLV	VRRFKKFFSKLKKLV	15	B1-L-5	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: Huh-7 (IC50=13.84±1.53 μM); MCF-7  (IC50=13.99±0.73 μM); U87 (IC50=14.33±2.35 μM)	HC50>80 μM	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ(2) and Ⓧ (9) are cross-linked by hydrocarbon stapling	L	Not available	35464194	 Erratum in: Front Chem. 2023 Oct 27;11:1285120. 	Su Z, Liu C, Cong W, He S, Su L, Hu H.	Design, Synthesis, and Antitumor Activity Study of All-Hydrocarbon-Stapled B1-Leu Peptides	Stapled AMP					
DRAMP35854	VKRFⓍKFFRKLⓍKLV	VKRFXKFFRKLXKLV	VKRFRKFFRKLSKLV	15	B1-L-6	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: U87 (IC50=4.58±0.93 μM); MCF-7  (IC50=5.152±1.04 μM); Huh-7 (IC50=5.89±0.43 μM)	HC50>80 μM	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ(5) and Ⓧ (12) are cross-linked by hydrocarbon stapling	L	Not available	35464194	 Erratum in: Front Chem. 2023 Oct 27;11:1285121. 	Su Z, Liu C, Cong W, He S, Su L, Hu H.	Design, Synthesis, and Antitumor Activity Study of All-Hydrocarbon-Stapled B1-Leu Peptides	Stapled AMP					
DRAMP35855	VKRFKⓍFFRKLKⓍLV	VKRFKXFFRKLKXLV	VKRFKRFFRKLKSLV	15	B1-L-7	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: U87 (IC50=12.70±1.63 μM); MCF-7  (IC50=13.36±1.22 μM); Huh-7 (IC50=7.49±1.2 μM)	HC50>80 μM	Cyclic (Stapled)	Free	Amidation	Stapled: Ⓧ(6) and Ⓧ (13) are cross-linked by hydrocarbon stapling	L	Not available	35464194	 Erratum in: Front Chem. 2023 Oct 27;11:1285122. 	Su Z, Liu C, Cong W, He S, Su L, Hu H.	Design, Synthesis, and Antitumor Activity Study of All-Hydrocarbon-Stapled B1-Leu Peptides	Stapled AMP					
DRAMP35884	TSFⓍEYWⓍLLSXLAXYXP	TSFXEYWXLLSXLAXYXP	TSFSEYWSLLSXLAXYXP	18	SPMI-HIF1-1	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=82.1 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6684.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35885	TSFⓍEYWⓍLLSXLAXYXP	TSFXEYWXLLSXLAXYXP	TSFSEYWSLLSXLAXYXP	18	SPMI-HIF1-1	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=85.1 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6684.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35886	TSFⓍEYWⓍLLSXLAXYXP	TSFXEYWXLLSXLAXYXP	TSFSEYWSLLSXLAXYXP	18	SPMI-HIF1-1	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=57.7 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6684.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35887	TSFⓍEYWⓍLLSXLAXYXP	TSFXEYWXLLSXLAXYXP	TSFSEYWSLLSXLAXYXP	18	SPMI-HIF1-2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=82.1 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6685.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35888	TSFⓍEYWⓍLLSXLAXYXP	TSFXEYWXLLSXLAXYXP	TSFSEYWSLLSXLAXYXP	18	SPMI-HIF1-2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=85.1 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6685.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35889	TSFⓍEYWⓍLLSXLAXYXP	TSFXEYWXLLSXLAXYXP	TSFSEYWSLLSXLAXYXP	18	SPMI-HIF1-2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=57.7 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6685.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35890	TSFⓍEYWⓍLLSXLAXYXP	TSFXEYWXLLSXLAXYXP	TSFSEYWSLLSXLAXYXP	18	SPMI-HIF1-3	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=82.1 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6686.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35891	TSFⓍEYWⓍLLSXLAXYXP	TSFXEYWXLLSXLAXYXP	TSFSEYWSLLSXLAXYXP	18	SPMI-HIF1-3	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=85.1 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6686.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35892	TSFⓍEYWⓍLLSXLAXYXP	TSFXEYWXLLSXLAXYXP	TSFSEYWSLLSXLAXYXP	18	SPMI-HIF1-3	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=57.7 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6686.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35893	TSFⓍEYWALLⓍXLAXYXP	TSFXEYWALLXXLAXYXP	TSFREYWALLSXLAXYXP	18	SPMI-HIF2-1	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=6.7 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6687.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35894	TSFⓍEYWALLⓍXLAXYXP	TSFXEYWALLXXLAXYXP	TSFREYWALLSXLAXYXP	18	SPMI-HIF2-1	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=10.8 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6687.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35895	TSFⓍEYWALLⓍXLAXYXP	TSFXEYWALLXXLAXYXP	TSFREYWALLSXLAXYXP	18	SPMI-HIF2-1	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=15.2 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6687.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35896	TSFⓍEYWALLⓍXLAXYXP	TSFXEYWALLXXLAXYXP	TSFREYWALLSXLAXYXP	18	SPMI-HIF2-2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=6.7 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6688.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35897	TSFⓍEYWALLⓍXLAXYXP	TSFXEYWALLXXLAXYXP	TSFREYWALLSXLAXYXP	18	SPMI-HIF2-2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=10.8 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6688.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35898	TSFⓍEYWALLⓍXLAXYXP	TSFXEYWALLXXLAXYXP	TSFREYWALLSXLAXYXP	18	SPMI-HIF2-2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=15.2 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6688.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35899	TSFⓍEYWALLⓍXLAXYXP	TSFXEYWALLXXLAXYXP	TSFREYWALLSXLAXYXP	18	SPMI-HIF2-3	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=6.7 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6689.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35900	TSFⓍEYWALLⓍXLAXYXP	TSFXEYWALLXXLAXYXP	TSFREYWALLSXLAXYXP	18	SPMI-HIF2-3	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=10.8 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6689.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35901	TSFⓍEYWALLⓍXLAXYXP	TSFXEYWALLXXLAXYXP	TSFREYWALLSXLAXYXP	18	SPMI-HIF2-3	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=15.2 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6689.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35902	TSFⓍEYWⓍLLS	TSFXEYWXLLS	TSFSEYWSLLS	11	SPMI1	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53-/- (IC50>100 μM); HCT 116 p53+/+ (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6690.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35903	TSFⓍEYWALLⓍ	TSFXEYWALLX	TSFREYWALLS	11	SPMI2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: HCT 116 p53+/+ (IC50=28.4 μM); HCT 116 p53-/- (IC50>100 μM)	Not available	Cyclic (Stapled)	Acetylation	Amidation	Stapled: Ⓧ(4) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36185610	Theranostics. 2022 Sep 11;12(15):6665-6691.	Chen S, Li X, Li Y, Yuan X, Geng C, Gao S, Li J, Ma B, Wang Z, Lu W, Hu HG.	Design of stapled peptide-based PROTACs for MDM2/MDMX atypical degradation and tumor suppression	Stapled AMP					
DRAMP35912	TEⓍQTNⓍVP	TEXQTNXVP	TESQTNSVP	9	SHP1	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(3) and Ⓧ (7) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13883. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35913	FRRKAFⓍHWYⓍG	FRRKAFXHWYXG	FRRKAFSHWYSG	12	SHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=37.21 ± 2.66 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13884. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35914	VPKⓍVNAⓍIA	VPKXVNAXIA	VPKSVNASIA	10	SHP3	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13885. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35915	VPⓍLTQⓍMF	VPXLTQXMF	VPSLTQSMF	9	SHP4	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(3) and Ⓧ (7) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13886. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35916	NEAⓍYDIⓍFR	NEAXYDIXFR	NEASYDISFR	10	SHP5	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Not available	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(4) and Ⓧ (8) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13887. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35917	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	FSHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=3.71 ± 0.21 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13888. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35918	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	FSHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=18.69 ± 1.27 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13888. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35919	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	FSHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=33.79 ± 2.34 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13888. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35920	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	FSHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=52.33 ± 3.75 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13888. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35921	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	ClSHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=3.71 ± 0.21 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13889. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35922	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	ClSHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=18.69 ± 1.27 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13889. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35923	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	ClSHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=33.79 ± 2.34 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13889. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35924	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	ClSHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=52.33 ± 3.75 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13889. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35925	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	BrSHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=3.71 ± 0.21 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13890. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35926	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	BrSHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=18.69 ± 1.27 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13890. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35927	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	BrSHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=33.79 ± 2.34 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13890. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35928	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	BrSHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=52.33 ± 3.75 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13890. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35929	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	ISHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=3.71 ± 0.21 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13891. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35930	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	ISHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=18.69 ± 1.27 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13891. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35931	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	ISHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=33.79 ± 2.34 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13891. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP					
DRAMP35932	XRRKAFⓍHWYⓍG	XRRKAFXHWYXG	XRRKAFSHWYSG	12	ISHP2	Not available		Synthetic construct	Antimicrobial, Anticancer		No specific results about the strcture presented in the forms of tables, graphs or words	No other descriptive information about the structure found in the literature	Not available	Not available	Tumor cells: MCF-7  (IC50=52.33 ± 3.75 μM)	Not available	Cyclic (Stapled)	FITC, 5(6)-carboxy fluorescence	Free	Stapled: Ⓧ(7) and Ⓧ (11) are cross-linked by hydrocarbon stapling	L	Not available	36240440	J Med Chem. 2022 Oct 27;65(20):13866-13891. 	Adak A, Das G, Gupta V, Khan J, Mukherjee N, Mondal P, Roy R, Barman S, Gharai PK, Ghosh S. 	Evolution of Potential Antimitotic Stapled Peptides from Multiple Helical Peptide Stretches of the Tubulin Heterodimer Interface: Helix-Mimicking Stapled Peptide Tubulin Inhibitors	Stapled AMP